プライマーセット冷水病菌
Contents
1.2. Exp Date PP Loopamp 48 20C PMO0006 93 1 2 3 4 5 6 7 8 Notomi T et al Nucleic Acids Research 28 No 12 e63 2000 Nagamine K et al Clin Chem 47 No 9 1742 1743 2001 Mori Y et al Biochem Biophys Res Commun 289 No 1 150 154 2001 73 2000
3. 3 60 Positive Control CWD PC CWD 0 DW ee PCCWD 0 10 20 30 40 50 60 Positive Control CWD PC CWD 2 Loopamp LA 320C 2 gt 1 240 260nm 350 370nm Positive Control CWD PC CWD Distilled Water DW
4. 4 Loopamp DNA 1 1 Loopamp 20 gL 2 DNA Positive Control CWD PC CWD Distilled Water DW 5 L 25uL 8 2 1 Loopamp LA 320C RT 160C 0 5 65
5. 65 60 80 C 5 95 C 2 2 3 A NS Loopamp gt 1 Loopamp LA 320C RT 160C 2 Positive Control CWD PC CWD Distilled Water DW
6. 20C 20 Loopamp Loopamp LA 320C RT 160C
7. 2 3 Eiken GENOME SITE URL http loopamp eiken co jp 1 10 ll1 12 13 14 77aro ac ez777 psychrophilum
8. 23 2000 26 2003 41 67 71 2006 16 3 0120 308 421 cd 2 72 143 380039 A
9. 0 5 10guL 10 100guL 100 1 000L 8 Loopamp Loopamp LA 320C RT 160C Loopamp DNA 2xReaction Mix RM Polymerase Bst DNA Polymerase Distilled Water DW 2 DNA DNA DNA 2 8 80 DNA
10. 95C 5 2 000xg 30 900 6 6 s7 DNA 3 1 20C 2 Loopamp DNA 2xReaction Mix CRM amp st DNA Polymerase Distilled Water DW Primer Mix CWD CPM CWD gt 1test gt 10tests gt 2 x Reaction Mix RM 1 2 35 125 L Primer Mix CWD PM CWD 2 5 uL 25 uL Bst DNA Polymerase 1 0 uL 10 aL Distilled Water DW 4 0 wuL 40 gL 20 0 wuL 200 L 3 Distilled Water DW 4uL 1gL Loopamp Distilled Water DW 3L 20L Loopamp
11. 380039 A 2008 2 1 toopamp LAMP Loop medijatad lsothermal Amplicatlnn Primer Set for Flavobacterium psychrophilum LAMP Loop mediated lsothermal Amplification 1 2 6 4 primer 9 0 Loopamp DNA Flavobacterium psychrophilum PPICCpeptidyl prolyl cis trans isomerase C 1 Primer Mix CWD PM CWD 2 Positive Control CWD PC CWD 1 48 0 12 mL 1 tube 0 1 mL Xx 1 tube 1 0 5 mL 1 5 mL
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