Biacore Q 日本語取扱説明書
Contents
1. gt N2. Biacore Q Instrument Handbook 15 3 1 90 3 1 1 CM5 N g CM NHS N NHs COO co COO COO COO COO COO_ 0 COO COO COON CONHCH CH
3. pH 0 5 2 pH 50mM NaOH 30 BidcoreO Instrument Handbook
4. pH 30 1 BidcoreO Instrument Handbook _41 i Run gt Run Sensorgram FInwcell ancel Mu Nehuark PF File hame assayll Sawe as tube Results Fr Carncel 2 BidcoreO Instrument Handbook C NBIA Uses Sqve Command gt Inject Tniectinn 5tart Imlechinn Eahcel Injection in Fc 1 IINJE
5. Edit Add Report Point dd Report Point ehnsogram Fc 1 Time ls do E 3 en ld OK 0 Baseline Biacore Q Instrument Handbook 12 RU SOOOO SI UI 35IHI 3 25IHI 3 2 15 nn 10000 SH D Dn 5 100 150 Time Fc Time bsHesp Slope Baseline lesg ld 1 88 5 13963 1 0 01 Yes 7 0 paseline RelResp 0 RelResp
6. 5 2 Desorb 29 BIAdesorb solution 1 2 ml BIAdesorb solution 2 2 ml 11 mm Sensor Chip Maintenance 200 ml Biacore Q Instrument Handbook 5 8 Desorb Start Undock th Desorb Flace thermo rack F In rack base 2 right and g mdesorb snluhinn 1 Im positiorn HH g ml BIadesnrh snluhnn 2 Im position HgLE Insert 5ehsnr Chip nhEhancg Carncel Undock Sensor Chip Maintenance Continue 27 Desorb 3 Desorb The higsnrh pocedre is completed Humninn stabilization procedre Fiecommehded mimimum stabilization him db Llick Stop to stop the stabilization hrncEHure Desorb
7. Sensor chip Dock Dock Sensor chip Dock Undock Sensor chip Dock BidcoreO Instrument Handbook 4 1 2 2 Prime Dock Working Tools Prime Working IE x esorb and Saritize FT Fanece 5tandhy
8. Edit Delete Edit Insert File Edit Run INJEET R2A1 20 y How En ff INJECT R2B1 5 Command Oueue Command Stop Inject WASTE Injection Stopped 3 Hgmall Sample Dispense remaining sample to Biacore Q Instrument Handbook 3 2 lt gt
9. Multi sample Samples 1 BidcoreO Instrument Handbook 69 Samples Select Al 5taluls Tupe hp deskjet SE series HETE SB nm Heport sections Sample ahlg Calibration Lurye 1 Lalhralinn Lurwe 2 Calibration Lurwg 3 Calibration Lurwe 4 Calibration Lurye verade Trend Flot Help ah Report sections BidcoreO Instrument Handbook 2 Run Run Project Ji Hianr Lnntrnl software nn COM1 File Edit wiem Command Run Tools Windnw Help RUN Sensorgram lt 2 Run Froject
10. Threshnld yalua Evaqluation settings Sqmple unit No of significant digits Threshold value negative positive Next gt Fonrentratinn Analysis Conditioning Sehnsor chip Bunsensor chip conditioring Golution aig I5nmk iaIH lt No of inections 5 Corntact IIme EE Flow rate ln Imml tahillzatinh hme Flnw sustem Hun low smstem conditioring 5nlutinh hame bummy No of cucles Help lt Back ewt Llnse Sensor chip Run sensor chip conditioning BidcoreO Instrument Handbook Solution name 2 5 No of injections
11. Biacore 1 1 Run Start New Cycle Start New Cycle Ir Run Stop Sensorgrum bulk effect bulk effect Bulk effect BidcoreO Instrument Handbook 3 2 2 Wizard
12. 4 Stop BiacoreQ Instrument Handbook So _ 5 3 Desorb qnd Saqnitize 77 BIAdesorb solution 1 2 ml BIAdesorb solution 2 2 ml 11 mm BIAdisinfectant solution conc 1 5 ml 20 ml Maqintenance Kit 30 ml Sensor Chip Maintenance 200 ml Desorb and Sanitize Start Continue Undock Desorb and 5anitize 8 Flacg thermo rack F in rack base 2 rght nd mtdesorb snluhnn T in nnslhnn HgLB g ml Badesnrh snlulinn 2 In nnslhinn HgLB Insgrt Sensor hin aimtenarce La hc Undock Sensor Chip Maintenonce
13. 4 2 Command Undock th Undock x Cahncel Sensor chip 4 3 Biacore Q Control Software BidcoreO Instrument Handbook SN 5 Biacore Working Tools Tools gt Working Tools Biqcore Maintenance Kit Surface Prep Wash Working Tools 1 x Desorb and Sanitize Frime chutdown Cancel Surface Prep Wrash Hemoves adsorbed protelhs Total ruh hme ts about 29 minutes followed by a recommended stabilizatiorn hme of 4 hours Last Hun Time 13 Nov 02 20 05 5 1 Prime 7
14. Wizard Run Run Project Biacore Control software on COM1 File Edit Wiem Command Eun Tocls Windnw Help law Project law Folder nwEl I nlHET Projects unHer dEwelnhmghl ll saltinns in the termplates car be modified Help Ha Ep Close BIA Projects Project P21Project Development Project Next gt BidcoreO Instrument Handbook HIA Prnlecls ew Frnlecl hinh bining Mew Folder Fnlic acid Witamin B12 nrE li nlHET EIE Frnlecls uhder deyglnnmehnt all settings in he emplates Car be moditied Help SHck Ew Llnsg New Project iPrnjers NEw Project E x Hengral Description Froject ang Short descriptior Fassword for published project Enable hasswnrd Password Contim password Fassword for dawelnhment project Enahls password Password Lnnirrm password EI 5 nt0 wi Hglp Fnint aw
15. 20ug ml 10mM pH 6 100 Hll 10mM pH 5 100 Hl 10mM pH 42 100u Run gt Run Sensorgram eh Flowcell x LE arr OK Set Flom Flow Llymm File name assanll lt awe as lupe Results blr Carcel 2 C BIA Users IE BidcoreO Instrument Handbook 19 Saqve Command gt Inject I Injection njection in Fc 4 INJECT lt amhlg Fositior IBFai lumg 1 a Contact Time El Lnrsumhlinh dU nrg gt Start Injection pH
16. Prime Tools Working Tools HBS EP 10 mM HEPES 0 15 M NaCI 3 mM EDTA 0 005 Surfqctant P20 pH7 4 HBS P 10 mM HEPES 0 15 M NaCl 0 005 Surfactqnt P 20 pH7 4 HBS N 10 mM HEPES 0 15 M NaCl pH7 4 0 22 um Biacore Q Instrument Handbook 6 1 2 3 Command Rack Base Rack Base 7 OK BidcoreO Instrument Handbook 7
17. Pe OFF TEL 03 5331 9336 ED 9 00 17
18. lt Surface Prep Dock BidcoreO Instrument Handbook Dock Surfqce Prep Surface Prep Biacore i Control software on LOM1 File Edit Wiem Command Eun Tocls Windnw Help RUN Sensorgram 2 Run Project Run Project a Projects 5ElErE Project Select project Development Puhlished Hew Proect Fnlic acid New Folder MULE Mnwg Fnldgr BI Hi Delete uanhicahinn of WItarmm BE in toods nk al gt Llnsg
19. Min Vol ul Next gt Concentration Analysis Prepare Run Preparation for analysis 1 hake sure the correct sensor chip IS docked 2 Add reagents and samples to the followying positions REAG _B reacgernt rack position Position Min Vol ul Content 2 55 Duimimy 4 3500 SOmhd NaoH 5 3275 SHm NaoH THERMO_F right hand rack position Position Min Vol ul Content 1 175 sampleaA 1 1495 mouse IdG Snginl C1 14 5 mouse IgG HH781 ng Help Menu lt Back Next gt Llose Next gt Next gt Start gt Start gt Save im Development lt History Desktop hy Documents File name asa lt Mu Network F Save as type war template blw Cancgl Wizard Development C BIA Projects Project Concentration Analysis Development Biacore Q Instrument Handbook 59 Saye Wizard Results As x tT Development Uocum Mu Computer ke
20. P53 Next gt BidcoreO Instrument Handbook Concentration hnalysis Report Foinkts Heport poirnts Tirme Hefore Start of Window Baseline 1 Before Start of hain Iniect RS YBS RESh TS 3 fEF Erid nf haln Iniect RelResh Baseline plLlm Hglp Ei Delete lt Back Mut Llasg Add Concentration nnalysis Add Report Point Time Beforesstter Start of End of Inectior DE Hglaliwe to Mindnw Pla ro F Help Lanrl OK Report point Id Concentration hnalysis Calibration Farameters Cahbratiorn pornts Fos CnrnrErtratinn Replicatess pgrmll hrahin Eurye nalute hame mause glgcular weight T150000 gmol Fittirg Funchar 4 Farameter Equation Hun nrder As Entergd Hghnr
21. 1 1 1 Replicate 1 Concentration 10 8 6 4 2 1 0 10 8 6 4 2 54 Next gt Hum settings aw nn of samples Icluding replicates Ewaluation settings Sample wrt nk Ho of igritieant digits 3 rk Threshold walue Hep Menu lt lt Back New gt gse Run settings Max no of samples Evaqluation settings Sqmple unit No of significant digits Threshold vaqlue negative positive
22. BiacoreQ Instrument Handbook 6 System check 1 Desorb aqnd Sanitize BIAtest solution 1 ml Sensor Chip System Check HBS EP Biacore 200 ml Sensor Chip System Check Dock HBS EP Prime Rack base2 Thermo_F Tools Test tools Ts Tnnls nal ualtu cheEK 0 0 i st check nstem check awaluahinn ac Checks Hetechinn uhIt Ii cell ctnrg pimp uhchinn autnsarnnler herfnrmmahcE ar FL samnle Innh and walees Total run hme is hnut 1 haur HOTE This nrncedurg shnuld be run at SC wlth a Sustem Check Chip Last Huh Time HI8 Mau HH 1 System Check Start Gystem Check This nrnceHdurg tests the Hgtechinn urilt ard the tntal lnuld handlirg sustem Total ruh hm is about 1 hnur Riequired snlulinns rom Biacore ainternahce Ft Hategt snlutinn HB5 EF hulfer IA Lartiied This nrnceHure shnuld
23. Esclude sample Edt Wi ngle Sample Help gg Sample GrnuD Sqmple Edit Single Sample Sqample Sample Group Fnmrentrainn Analysis Edit sample Information Help EsInTE Laric n818 3n42 54 BB 78 Exclude sample Edit Concentration nitnl Biacore Q Instrument Handbook _67 i Concentration Analysis Results _ Hun Log Calibration Samples Trend Pl Notebook 1 19 21 2 3 Cycle Heport point Baseline Linear regression Close Trend Plot Report point Concentration Analysis Results
24. Min Vol pl Min Vol ul Biacore Q Instrument Handbook Sr Immabilization Prepare Run Preparation far immobilization 1 Place the Surface Prep uH in the Fright harnd Fack postion EE EMFE the Correct sensor hip EE docked 3 Feagents tn the followring postions THEFRG F lett harnd Fack position Position Min Vol tul Content 1 172 at RERG CU readent rack hnsrtinn Pnsrinn Miin nl tul Content 1 5 EDC Help gnu ack gt Llnsg Next gt Next gt start Start Biacore hin unu want to sawe CUTent wizard template awe wE 3 Save As Development C BIA Projects Project Immob
25. 3 Next gt BidcoreO Instrument Handbook iTmmnhilizatian Injertion 1 31 Inection tnne lniect fim and inject in and Int settings 5nluhinh hame 1 EDC Fractior 5n luhinh nam HS Frachinn BI Lnrtact tirme 7 rmirn Flow rate 0 petri lt ack IE Di Llnsg EDC NHS Injection Type Mix and inject Fraction EDC NHS 50 Contacttime 7 Flow rate 10 ul min Next gt Extra wash after injection with Immabilization Injection 2 t3 E _ Inlgchinn tnne lnect Mig and injgct nect setthings 5nlutinh ae B2 Herlyallwg Lnntacl hme a rmin Flow rate pele rran ww Ewtra wash after inectiorn il ater lt Hack 1 Close Solution name Contact time Flow rate Extra wash qfter injection with
26. Surface Prep 88 Surface Prep Undock BidcoreO Instrument Handbook 3 2 3 2 1 5 ng ml ug ml Regeneration
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28. Next gt 51 iLnnrenLratinn mnalysis Main Inject Inlgclinh tnng Iiect im and imjecl Ms slhn s Mig mr Frachiarn In uick mis hi airn ingct sglhn s Contact hme 3nn Flnw rate 10 ILmnl Help Hack ggl s Close Injection type Mix and inject BidcoreO Instrument Handbook Mix settings IgG 75 Next gt Concentration Analysis Regqeneratian x Fieqeneratiorn slhnns Golution mame 5Drmkd MaHH Contact hmg E Flow rate En lmml Exhra wash after imlgchinh tabilization mg after nectior l Help lt Hack gw gt Close Next gt Cancentration Analysis Report Poinks 4 Heport points Time
29. pH4 0 SA 10mM 50mM pH 8 5 1M NaCl 100ug ml Biacore Q Instrument Handbook 17 3 1 2 CM CM
30. Pre Inject BidcoreO Instrument Handbook Enhancement Concentration hnalysis Main Inject nection tnng tr lnject is and inject hd airn InIEct settings Corntact fime 3nn Flnw rate 10 li Help Back pl Close Injection type Inject Next gt iLnncenLratinn Analysis Regqeneration Fieqehneratonhn settings Golution name 5Dmkd MaHH Lnrntact hmg 5 s Flow rate lgn pl Ertra wash after iniection Stabilization hime after nectorn l Hack l Llose Next gt CF Concentratuag hnalysis Report Pnints Tirme Before tart nf WWindnwy 10 Betore tart nf lain Iniect ter End nf ain Inject RE aseline umi E Delete Hack Menl gt Llnsg
31. F OD HH 60 4 Z 2
32. Biacore Q Instrument Handbook 35 1 Immabilization Injection 3 31 rectiorn tnng lnject im amd inject nect Settings lutinh harme IEtnanoamme Corntact img E mm Flow rate ho pirrin Eztra wash after inection mh water lt Back Magt gt Llose Contact time 7 Flow rate 10 l min iTmmmnhilizatinn User Tnfnrmmatinn EE sgr drminstrator Flow cell 7 Sensor chip has been docke i OOhrs eats aOR aC oof Ta ntebook Hglp Hack Di Close Sensor chip has been docked Next gt Notebook Biacore Q Instrument Handbook EDC IHS Mi position min cabacity HE Hg darlwatiwg Etharinlarmine ITHEHMnF ssc Nane OOO 9 2 ols OO Oad ke sure that the correct rackls are installed in the autnsamnler when nnu start the ruh _ Hep _ Menu Hack ent Close
33. Projects Select Wizard a x Froject administration Projecth assaull1 Wersln dewelopment Froject Properties Publshed Yersians Select wizard This wizard guides nu Gurface PErfarmance Wizard hrnunh the steps in ssay Dewelopment Wizard iImmobilizing iand or he Eoncentration snalysis Wzard SENsOr Chip surface Bark ggt gt Llansg Published Versions Biacore Q Instrument Handbook 97 El Projects Publshed ersions _ lt asa 50 Fublsh New Version Help Fr Project Publish New Version a Projects Puhlish Mew WErsinn hiEr Project a em wersInh 1 Fublished bu Narin Effectwe date 5 D5 lt Heasnn for riginal wersioh He wersior Termplates to publish LL mebae elect Template aw Carncel Project Wizard mmobilization Surface Preparation Concentration Analysis Wizord Select Template Biacore Q Instrument Handbook 98 a Projects Select Template elect template to Use fo
34. Sample Id Amount g Volume ml Dilution Factor Dilution Next gt iLoncentration Analysis Rack Positions bB SOmhd ET mouse ld mouse Id mouse Id mouse ld mouse Id THEHM _F OOOOO OU hlake sure that the correct rack s are installed in the autosampler when wou start the run Help Menu lt Back Next gt Llose O O O wii BidcoreO Instrument Handbook Min Vol
35. Biacore Q Instrument Handbook 100 7 3 Project BIAuser Run Run Project Projects Select Project ssaul Frnlgclg for outline ug Dn urrshEclhic salhIh g Ca be modified Hack E Llose Project Next gt a Projects 5ElErF Wizard a Frnjgcl adminislrahinm Frnlect ap Wgrsln 1 clwg eh Frnject Frnnerlies Publshed Versins Select wizard This wizard guides nou through the steps In irmrmoabilizing li ard or the SENsSOr Chip surface Back Mawl gt Llnse Wizard Next gt a Projects Gelect Template EE Emishinn template Open termplate In this project lt No temnlates awalahlg Back ggt gt Llnsg New template Next gt Biacore Q Instrument Handbook 101 iTmmmnhilizatinn User Information Hghgral Llsgr nrin Flow cal Mnlghnnk Flow cell Next gt Notebook 5 1 Immabilization Rack Positions NHS
36. Run Run Project Ji Hianr Lnntrnl software nn COM1 File Edit wiem Command Run Tools Windnw Help RUN Sensorgram lt 2 Run Froject Projects Select Project Gelect project hiewalnnmant Published gw Project ew Fnldgr B Folic Be Mnwe Tn Fnldgr Protein ht itamin B12 Lielete Ihhihiinh assan Hglp HG Mawl gt Llnse Project Next gt P21Project iProjecLs Select Wizard EE Froject adminislrahnn Froject Protein a WIn deweloprment Froject Froperties Publshed Varsians Select mizard lmmabilizationwizard his wizard guides you Ehrough the steps in testing ssay Dewelopment wizard suface actity Eoncentration nalyss Wzard Help sak at s Clnse Surface Performance Wizard Next gt BidcoreO Instrument Handbook Projects Select Template _ Esishnn template Open termplate In this project Modified No templates awailable Help lt Back aw gt Clnse New template Next gt nectiorn seguerhnce Mair imjact Hun
37. Next gt BidcoreO Instrument Handbook nluhinri name Im olacirnelpH1T 5 lo of inections 5 Contact IImg len Flow ralg Ian lmml Stabilization hrng ln Flow sustem Fun Haw gwstem cnndilinnin olution an hiumm No of cucles Help lt Back est Clase Sensor chip Run sensor chip conditioning Solution name No ofinjections 2 5 Contact time Flow rate Stabilizationtime Flow system Run flow system condittoning 3 Solution name 0 No of cycles 2 10 Next gt BidcoreO Inst
38. FI Freconcehtration of mouse pH 4 gLHn 1EHnn Ti Lm Lm 14dnnn Responst 1 Run gt Stop sensorgrum pH NHS pH8 5 pH4 5 pH 5 pH pH pH5 BidcoreO Instrument Handbook 3 1 3 IFC Wizard IFIC Surface Prep
39. Stqrt Injection BidcoreO Instrument Handbook 10 RU lll 35HI 3UHH 25000 20000 R 15nnn 10000 In Sn 100 15n 200 Command Inject 1S000 10000 D i Run gt Stop Sensorgram BidcoreO Instrument Handbook 11 2 2 RU View Reference Line 40nnn 35nnn 3nnnn 25nnn 2nnnn 15nnn 10000 SI SH 100 150
40. Next gt BidcoreO Instrument Handbook iPFnjerts Select Wizard Frnlgcl aHminisalinn Frnlert Protein a WersIor deweloprment Froject Froperkies Publshed Varsions Gelect mizard Irmrnobilization izard This wizard gudes wou Gurface PFgrfnrmance Wizard through the steps Im sia Developrment Wizard Corhcentratoh measurermeht Enncentralinn tanalysis Wizard Includinn determination of a calibiration cure Ewisting template Oper termplate In this project lt Madiied ho termplates awallahlgs Help Hack Mggt gt LlInsg New template Next gt Concentration Analysis General Settings _ njectinon SBHLIETICB FF hdain inject Enhancemert I Regeneration ho lt _ Hep _ lt Back New gt ese 1 Main inject Regeneration Next gt
41. amp Rubber caps Type 2 20ml 16mm 4C BIAdesorb solution 1 Biacore Q Instrument Handbook 94 7 7 1 BiacoreQ Control Software 2 7 1 1 Windows 2000 Biqcore O 3 BIAqadministrator BIAdeveloper BIAuser Biqcore O PC Computer anogcmcnt SS Action View Tree Name UU escrption Computer Management Local Administrators Administrators hawe complete and u System Tools Backup Operators Eackup Operators can override secu Eyen Viewer 4 Guests Guests hawe the same access as Mme 3 System InFarmatian Power Users Power Users possess most administr PerFarma
42. Menu vr Llose Help Close Staqndby Run Tnnls Windo Help FF1 2 tart 5andhy 8 CE A Tools gt Stop Standby Standby Close 68 BidcoreO Instrument Handbook 83 4 4 1 2 1 Standby 4 4 Standby Tools gt Start Standby 5 ul min 4 96 96 Standby 80 ml Tools Stop Standby Tools gt Working Tools Prime
43. Continue 27 Desorb Biacore Q Instrument Handbook 87 Desorb and 5anitize EO x Flace the Badisintectarnt snlulinn i the hulfer cnmnartmert Pd insert the nlet tubes Irnm both pmps Carncel Desorb BIAdisinfectant solution Continue 34 Sanitize Diesnrb and Sanitize EE x Feplace the Eladisintectant snlulinn th a bottle cnntalnin at least TO ml dinhised and deqassed mater TIREL hin not stop the sustem at this point 7 Desorb and Sanitize The Saritize hrnceHure Is completed Humnhinn stabilization proacedure Fiecommehnded minirnurm stabilizatior tire 4 hours Sanitize 4 Stop BiacoreQ Instrument Handbook 5 4 Surfqace Prep Surfaqce Prep Wash 21 Surface Prep
44. Un 35IIn 3UHUH 25IIn F nnnn 1S000 10000 SD DD In D Sn 100 15n 200 Fc Time mbsHesp pe Baseline FelFiesp Hi 1 Sh 1396531 DO Tes DD baseline 1 131 5 EdD 1 2 25 Mo ia BAtest solution View gt Reference Line BidcoreO Instrument Handbook _13 Window 2 Delete Command Queue
45. Project Project Project Project Assay Development Wizard 5 1 Project 2 3 BIAadministrdtor 4 Assay Development Wizard 5 BIAuser Dock Undock Working Tools Toolbar Toolbar BIAqdministrator BIAdeveloper Biacore Control software on Cor1 Flle Edit Wiem Command Run Tools window Help BlAuser Blacore CQ Instrument Handbook 96 7 2 Project BIAadministrator BIAdeveloper Run Run Project Biacare OQ Control Share on LOMI Fe EK View Cowmmand Run Tools Window Help Projects 5elErF Project Select project Dewelopment Published HIA Prnlects ew Frnlecl law Fnlder Mnwe Tn Fnlder Delete Hacki gt gt Llansg Development Project Next gt
46. Lk4 50 1 LL L LLL d 50 2 lL 70 83 OR f f 83 RIF WTO El 83 EO 27 83 Sn RL i bibi 84 SC IT oN 84 SAFR TAL SOG 84 Biacore Q Instrument Handbook 2 GAON TL 86 4SUNGEE Pre TD ENRON TLR hi i 88 AT LCDS cd 90 iG 94 7 1 4 94 711 a zs EA lt lt lt k Ss mMm m AM 94 7 1 2 Project EKx JRW CIYKYEIIOAOOOOOO OOOCOCG 94 7 2 Project DN BlAadministrator BlAdeveloper 2 96 7 3 ENE Project DE BAUsSer ee 100 Biacore Q Instrument Handbook 1 1 1 1 1 1 1 gt gt gt 30
47. Run information Event log Biacore Q Instrument Handbook 79 iLoncentration Analysis Results A Cycle Resp Data Calc Quality Conc RU nM y Mc K 85 OK 9 5 350 8 O 9 59 355 1 9 51 Hun Log Lalibratian Samples Trend Plall Notebook 19 1 210 2 OK 17 8 19 1 189 1 OK 20 2 199 5 19 0 38 1 107 2 OK 39 1 38 1 10B OK 39 3 107 0 38 2 5B 2 54 3 OK 74 3 7B 2 53 8 OK 5 1 4 1 74 7 152 4 42 3 Ook 152 4 1 2 OK 41 7 OK 100 150 Concentration Help Menu LClose Calibration Cycle Conc ug ml Calibration Solution Resp RU Datq Quality Caqlc Conc CV CV Exclude x x
48. a Projects Select Project Select project Dewelnpmert Fublished em Proect gw Folder Tolic acid kinwe Tn Folder UMWet in B12 Lslete Wi Inhihitinn assan Help Hpk Magt LlInse Next gt P21Project PFnjerts 5elect Wizard EE x Frnjgct adrministration Froject Protein a WgrsInh dewelonpment Froect Froperties Publshed Wersions Select wizard Immnbillalian wizard This wizard guides nu Surface Performahce Wizard through the steps in a Development wizard COrncerntraton measurermehnt oncentration halpsis za including deterrmination of a calbration Cury Help Concentration Analysis Wizard Next gt BidcoreO Instrument Handbook Projects 5elErt Template Existing template Inper temnlate in this prolect Madified No templates awalable Help lt Back gt gt LlIasg New template Next gt i Concentration Analysis GeneraliSetbings nectinn sEBHLIEBHCB Main inject Enhancemernt I Regeneration hn lt Regeneration
49. Contact time Flow rate Stabilizationtime Flow system Run flow system condittoning 3 Solution name 0 No of cycles 2 10 Next gt iLoncentration Analysis User Information ieneral User id Administrator Flow cel 1 vr hax no of samples In this rurn E Notebook Help lt Back Next gt Llose Flow cell Max no of sqmples in this run Notebook Next gt BidcoreO Instrument Handbook Concentration Analysis samples Sample ahlg Ni Imnurit Dilution Replicates gj Factor Help Positioning lt Back Mewt gt Close Sqmple Table
50. Rack Base1 Rack Bqse2 Thermo_F Reqg B Reag C 3 96 Rack base1 Rack base2 Themo_F 96 Reqgent rack base Reag B Reag C Thermo_F lt 16mm Reag B 7mm 11mm 16mm Reag C 7mm Te Ne ee Ca es NO SW CC SR EL ON VEN EN NG GS As GNU EE DGG IGG CGC NE GA IN SNS es NC NE NN GZCA SI SSL EE oy RS i a 5 MAG OO OMNIGOD a el ne eal ea Rack base 2 Rack Base1 Rack Base2 f 2 r2f2 96 Reqg B
51. 50 isopropanol 10 formic acid in water solution1 4 ml 50 DMSO in water solution2 4 ml 16 mm Sensor Chip Maintenance 1 200 ml Dock Prime Rackbase1 Thermo_F Rackbase2 None Surface Prep Wash Start Gurface Prep Wash hinck the malntenarnce chip Im the Surftace Frep unlt and place lt in rack base 2 right ancel Surface Prep Maintenaqnce chip Dock Rackbagse2 Continue Biacore Q Instrument Handbook Gurface Prep Wash Flace thermo rack F Im rack base lglt arnd ml yash snlutinn in nnslhnn RT1D1 4 ml wash snlutinn 2 In hnslhnn R12 Laricl Start 21 Surface Prep Wash Gurface Prep Wash The Suface Frep ash nrnreHure is completed Fiermember to empty the waste heakgr Exit Surface Prep
52. Concentration hnalysis Repeat Calihi a WF epeat calibratiors a eyerWl sample cucles Usermay chnnsg more frE uent calibration One wial Different wials each time Help ee Carncel Repeot calibration Colibration dilutionw Calibration solution One vial Different vials each time Buffer Biacore Q Instrument Handbook 55 Hun selhn s an nn samples 21 Includinn replicates E walulalinr sen s Sample ul pgml nn of sI hlhicant digits 3 Ii rTil _ Hep en Eack Met Clnse Run settings Max no of sqmples
53. Linear rearessian Llase Trend Plot Report point Concentration Analysis Results Hun Log Calbralion Samples Trend Plot Notebook Notebook Help sg Notebook Menu Llase Biacore Q Instrument Handbook Concentration Analysis Results Run Lal Caibatonl sanoes Tedal Moetowl System information Eyent log Software Biacare Q Control Software version 3 0 Time Event Duration 185 09 42 Run start Instrument id 3003 18 09 42 Run preparation start 8 18 59 49 Run preparation end Fiun information 18 59 49 Calibration start 21 4 5H Calibration end 8 6 21 49 50 Analysis start Proiect Protein amp 22 38 30 Analysis end 22 38 30 Run end User id Administrator Wersior deyelopment Start time 203 1 23 18 09 42 2003411423 22 38 30 End time
54. rr1 2 rr2 rr3 3 rr4 ReogC rra1 rrb1 rrq10 rrb10 Rack base 1 BidcoreO Instrument Handbook 8 2 2 1 Biacore Maqintenance Kit BIAtest solution Run gt Run Sensorgram ir FInwcell LE arr IIK Carncel 1 100 l min OK BidcoreO Instrument Handbook RCC 5awe Results As esktn bP iw Documents 1 1 ave as Lu Resuls F blr 0 C NBIA Users Save Command Inject Injection Consumption
55. 10 l min 1 Immabilization Injection 36 3 rectiorn he re Injgct Mi ard inject Inlgct Gettings nlutinh nag Ethanolaming Lnrtacl IE 7 rmin Flow rate lmm Exhra wash after inection Help Hack awt Llnse Contacttime 7 Flow rate 10 l min Next gt BidcoreO Instrument Handbook a Immabilization Report Points tiwation 1 Before Start nf Baseline lmirnokailization Lewel 5 ter Er of 3Fd Iniection RelResh Baseline Eu Delete ark Mt gt Llnsg Baseline Immobilization Leve 2 Next gt 1 Immabilization User Information _ E Hearl serid Hmnistralnr Flaw cel Noteboonk Help Hack Close Nex
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57. 4 R1B3 S4 1 E Ric3 Help Continue Cancel 5 Continue BidcoreO Instrument Handbook 63 a Concentration Analysis Results 22 40 47 Run start Run prebaf on start EE RU prebar ton ET 3 5 GE hrgtinrn start IHBiHE Crtini rd II U2 nalysis stat BE Taly IS HHEGB Ru Erd Run Log System information Run information Event log BidcoreO Instrument Handbook 1 Resp Data Calc WW Quality Cont RU 8 CR 8 R est 4 11 1 7 11 4 QR Hr 11 2 HGH3 11 2 S10 Reshonse 15 1 1 15 1 147 15 5 157 152 151 2 OH 4 2 QR 1 313 mg ER 315 g 3 HH 3 1 5 2 1 2 Concentration 5 ER HE31 alhralinm curwe lt Escludg calibration point Help Mernu M Clo
58. IFC Run settings NHS EDC 3 Next gt BidcoreO Instrument Handbook 23 iTmnmnbilization Injection 1 Inlgchinn tnpe Irmct ix ard inject Miw and inlgct Settings nlulin hame HS Fractior BI 5nluhnh na EDE Frachnh Contact hime 7 ml Flnw rate In pdr Etra wash after iniectiors Help Back ggt Llnsa EDC NHS Injection Type Mix and Inject 50 Contacttime 7 Flow rate 10 l min Extra wosh qfter injection with Next gt iTmmmnhilizatinn InjErLtinn zf4 njeck him ard inject CH Gotion hame Protein Lnrntact IIme fr mnl Flow rate 1 lmiml Ezha wash after inectior Help Back ggt Llnsg Solution name Next gt Contact time 7min Flow rate
59. iLoncentratinn Analysis Rark Positions tiWe slellilril Fratain B1HinMi R1 C1 rg Prntain dL5inhMl R1 D1 eg Protein a 12 2Tni R1 E1 eg Protein Tfnhd Frotein B 1inhi Mlcn lt REAG_C lt THERMO_F lt OO OOOOOOGO 2 OOOOOOGO OOOOOOQO IGG COOCOGG oO OOOOOOGO sa OOSOSS S C Feg akg sure that the correct rackls are Installed in the aulnsamnler whern nnu start the run Help Manu Back Nasl Llose Min Vol pl Min Vol pl Next gt Concentration Analysis Prepare Run Preparation for analysis 1 hake sure the correct sensor chip Is docked 2 dd reaqernts and almhles tn the fnllnweiid postinns REAG Breadernt rack postion Position in Vnl tul Content 2 5 Lu 4 SD SH aoH SH aoH THERIM FririgHt hard rack hnsrinn
60. 5urFace Preh Wash Frimgs the liguld snstem hn Hshin numns FL amd autnsamnler wulh Hugr se Ihis nceHure after docking a new chip and when the hulfer Is Chahnged Total run hmg is about b minutes Last Huh Time eB Mau 11 1B Start PFI Xx Ths pracedure Hushes pumps IL and autosampler with buffer se this nrnceHure after docking a new chip ad when the butfer Is chahged Flace a bottle containing hulfer in the hntlle compartrment amd insert the Imlet tubes Irnm the hurnDs Cahcel Prime Start 1 Prime x Hgmialmih kirme mn OE 04 Frimin please wall BidcoreO Instrument Handbook 5 6 Prime eh Prime x The Frime nrncedure is completed Prime Exit Prime Prime
61. Before start nf Iniectinn fs mfter Endnf J aseline 1I Betore Star nf hain Iniect hsRBSh 3 er Erid nf ain Iniect RglRiesh Baseline HH Edit Delete lt Hack ant Llnsg P53 Next gt Concentration Analysis Calibration Farameters alhrahinn nnims Pns CnnrErtrtinn Replicatss InM MM Calibration curyg nalute rame Prateir nlgcular meinhE T4100 gmol Fittirng Farctior a Farameter Enuahinm Hurn oder Descending Seres Report point Hgshnnsg Help gnu Back Mewt gt Llasa BidcoreO Instrument Handbook 73 Caqlibration curve Analyte name Molecular weight Fitting function Run order Calibration solution Reporting point DID Calibration points Calibration solution Replicate 1
62. Exclude Include Include Biacore Q Instrument Handbook iLoncentration Analysis Results 3 Calc Calc Conc Amount nM nM 10 _ 109 MediuIm3 109 kediuIm3 118 MediuIm3 8 BI kediuIm3 Id dil 5SH SO 150 250 Exclude sample Edit gt Concentration Help Menu gt Sample Cycle Sample Id Amountg Volume ml Dil Fact Resp RU Data Quqlity Calc Conc Caqlc Amount CV CV Wizard Threshold value Thershold Positive Negative P64 XE Biacore Q Instrument Handbook _81 Cycle Heport point Baseline lt
63. Fc 2 1 5H Fc 3 Fc 3 13223n Fr 4d gg3 Fc 4 1 1 eae gg 15 JHU0 00 RUI TL i LI Lh FG pa titlew 57 SU RU Strley 7 0 RUIr igine Mix 1IRUI 11590H Mix RUI L1153 yerades TRUE 1171O Difference kk 7 Crilution factor 1 Hoiser Fc 1 U RU TP Fc lt f RU Fc 3 lt 0 6 RU je Fc 4 1 U RU Long term Slope RU s e Dn nn 20 05 RUIS Check Biacore Q Instrument Handbook Biaqcore Q Maintenance Kit BIAtest solution 65ml 15 w w sucrose in HBS EP buffer BIAnormqlization solution 30ml 40 glycerol BIAnormqlization solution 30ml 70 glycerol BIAdesorb solution 1 90 ml 0 5 w v sodium dodecyl sulphate SDSj BIAdesorb solution 2 90 ml 50mM glycine pH9 5 BIAdisinfectqnt solution conc 10ml Sodium hypochloritelconc HBS EP buffer 200 ml 0 01M HEPES 0 15M NaCL3mM EDTA 0 0059 v v SurfqctantP20 pH7 4j Sensor Chip maintenance 1 Sensor Chip System Check 1 Saniize
64. Llase Next gt BidcoreO Instrument Handbook Gurface Performance Prepare Rum dd reagErts tn the fullnwwind positions THERPMIO FrriHt harnd Fack hnsrinri1 Position Miin Vol ply Content a Biacore Cio nu want tn ave CUTehnt wizard lemplate 1 4 5awB av 3 nnt awe Flace Buffer in the Buffer compartment and insert the inlet tuBings trom hnth ELMES Flace ari Ity hnttle nder the waste outlet in the Huffer compartmert Hep_ Mew cese Start gt Save Wizard Results As _ Save in Deyelnpment History Desktop Mu Documenls Mu Computer File name regeneration lesl My Network F Sawye as type IFesus blr Cancel Save Biacore Q Instrument Handbook 49 gurFare Performance Results PP TTF uFaCE performahce Flow cell 1
65. ig position min cahacity 110 LI 11 Protein 110 Ethannlarming hy channes Wo make to the rack gettinng here ml not take effect un nu start he ruh Help gnu Back wBE Llansg BIAadministrator BIAdeveloper BIAuser Biacore Q Instrument Handbook H9 3 NN OU 6 DR 9
66. 0 Fun Log Calbralion Samplss Trend Plot Notebook Notebook Menu Llase Help Notebook HLnTrCETItrBHn nly ent Rn Lagl Cr ams Leerd Fhe Hasbest str rrurrentie Galle Emcmmg Conlio Sollynae er 0 Timm Eurmt mmrmmimm TE RU bart Le 1ELTFEH G RL EE a TE RU re har an Er Pun iormation EE tr iT ha mf msialhnT ED Cr or Brad 5 ED etre mt Proect Fmlemna 22 38 30 Mahi tnd MET ME eratorA ED Fr nd lmt res TTTT EY TE Ered rme TET TEEN Hm Ene me al Em BidcoreO Instrument Handbook Close Standby Hd Run Tnnls Windo Help 1 tart Standby mi Tools Stop Standby Standby Close Results Menu Save Changes IE k Calibration Farameters Print Wizard Results Print Wizard Template nb k Print Wizard Results FE Lanc Report type Single sqmple Sqmples
67. 2 BidcoreO Instrument Handbook 2 1 1 2 Start BIA Programs Biocore O Control Software BIACORE C Control Software on COM1T 0310_ 70054 Sensorgram bd File Edit View Command Hun Tools Window Help x 0 4 n Cycle Curve Sensorgram Fc 1 RU Cyanocobalamin 0 8 ng ml 18000 Tool bar Menu bar 17000 1E6000 15000 Sensorgram window 14000 13000 12000 100 200 300 400 SI BI MI SI II 1000 Time s Fc A amp APROG Time Wwindow AbsResp Slope Quality Baseline HelHHesp 1 Analyse 0 13431 2 0 01507 0 Baseline 1 Analyse 373 5 200 0 16673 9 0 88233 Ok No 3242 7 5SlopeA 1 1 Analyse 493 5 A400 0 16769 5 0 78408 Ok No 3338 4 Slope Report point table 1 Analyse 513 5 200 0 16862 4 0 73112 Ok No 3431 2 SlopeB 1 Analyse 784 5 5 U 13833 6 0 16030 Ok No A402 4 Hesponse StQtUS WIHOOW Eyent Log Time Info Timestamp 10 mar D0 11 26 00 LU Set Temperature 25 0 C
68. Event lo Wi nd OW I Temperalure 25 00 C _ 0 Rack 1 Left Mico 9 emnet Li Rack 2 HighH Micro Instrument Offline Rack H Reagent Heaa_B Sensorgram Fc 1 Time 917 5s Hesponse 13421 9 RU Flow Flow Cell Menu bar Tool bar Sensorgram window Report point table Event log window Sensorgram window x 4A Status window Biacore Q Instrument Handbook 53 1 2 1 2 1 Dock Dock Insgrt a new Ehs r chip Llick hinck when readr fter docking nu shnuld ru Frimg frnm WWrnrkimn Tools EI
69. IImgzml ss HII HIH1 5 HI i 5 D 5 D Fositiwe Fnsltiwe Fnsltiwe Fnsltiwe E nn 1 5 8 5 8 lengtig engtiwg lglg lengtiwg 5 94 5 88 5 8 latig latig latig lalyg 1 n3 1 n2 1 n2 Cycle Sample Id Amount g Volumel ml Dil Fact Resp RU Data Quality Calc Conc Thersould ug ml Positive Negative Calc Amount CV CV 5 BB 5 84 1nn HMI Contentration Llose Wizard Threshold value Thershold BidcoreO Instrument Handbook Sqmple Sample Id Amount Volume Dil Fact
70. My Network F Results blr 4 Save 22 40 47 Run start 22 40 47 Run preparation start Biacore Q Instrument Handbook Pouse standby 8 MgasurEmBht Ih rodgress The sustem will be paused after IEs End Run dn HT hin EET 5 Delete Fi nia Run order Frin WigT ETi LE COP Ent Menu Saqmples Add Delete Run Order Biacore Q Instrument Handbook 61 iLoncentrat
71. iin Vol tul Content 1 NHS a ES ED Mi Fussrtinn minifniir capacty 110 LI 4 110 Frotein a 111 Etharnalaimine 5 SHim lanH 3 SE thE ylalS wet CaPS A Insert the racks in the correct rack positions _ Hep _Menu Back Next gt Close BidcoreO Instrument Handbook Next gt Next gt start Start Biacore hin unu Wan to sawe CUTent mzard template Sawe wE 3 Save As Development C BIA Projects Project Immobilization Development Dont Save Gave Wizard Results hs Desktop Mn ocuments CT Mn LnmDuler Mip Mghwnrk FP Sawe as tube Results Fr anicl 2 Save Development C BIA Projects Project Immobilization Developmen
72. settings Mumher of test cucles ls Hun Documentatian Help lt Back ent Llose Injection sequence Regenerationino 1 Run settings Next gt 5 HfarE Performance Main Inject hai InIECt settings lulinh ag Ian In Lnrtact hmg ln Flow rate 1d pln Help Back LInsg Next gt Biacore Q Instrument Handbook Er5urFare Performance REnEnEraLtinn 1 EE Hgnereralin settings Solution hame nm lycinglnH1 Corntact Im ln Flow rate 4n prirn Esta wash after inection Gtabilization Im after inectior Help Hack Mggl 5 Llnsg Next gt Gurface Performance Conditioning 8 Sensor chip ly Hun sensor chip conditioning 5nlulinrt hame Im glucinelpH1 5 lt IIECH IE 5 Lnrtact hmg lm s Flow rate Im lymm Stabilizatiorn me In Flo sustem Run fow system cnhditinnina nluhnh name p umrnn ho o
73. 00 C Rack 1 Left THERM _C BOOT AE Riack 2 Right THER MO a Hack Ri Reagent Reag A File gt Close Run Tnnls Windo Help 1 Stat Standby a stop standby EE Tools Stop Standby Standby Close Biacore Q Instrument Handbook 29 3 1 4 Surface Prep Surface Prep BiacoreO IFC Surfqce Prep Surface Prep Acetonitrile 100 Dimethylfomamide DMF 80 30min Dimethylsulphoxide DMSO 100 30min Dioxane 50 30min E
74. 0H lt gt 1 BidcoreO Instrument Handbook EDCIN ethy N 3 dimethylaminopropylcarbodiimide hydrochroride NHS N hyd
75. BIA Projects Project P21Project BidcoreO Instrument Handbook Next gt Projects Select Wizard Frnlect adrmimlslralinn Frnlgct Lamn Bg Wersln Hewglnhmeht Frnlgct Prnnerhies Publshed Versians Select wizard mmobilization Wizard his wizard guides wou Suace SE Wizard through the steps in ssay Development Wizard Immnhllizinn ligarnd on the Corncentration nalnsls Wizard sensor chip surface lt Bark gt Llansg Immobilization Wizard Next gt role 5elErE Template Enisting template Open temnlate Im this project Madified ho termplates awailable Hack Mi Close New template Next gt Immabilization Eneral settings PgrFarm Innhllz hnh in Hum sellim s Humber of inections 3 r Run Documentation Help lt ark Megt Llnsg Perform immobilization in Surface Prep Run settings NHS EDC
76. GE Healthcare Biacore Biacore Q Instrument Handbook GE Imagination Qt work CSRS hi 1 TO 0 0 N 1I66 1 1 1 1 1 1 1 2 2 LFAOU HE hi 3 152 HX OO NN 3 1 2 2 Prime 4 30 2 ck lt 6 2 ee 8 2 0 EE ml YKYEYIIEOEO7O711I7f If 7 So 8 22 DE 11 1 2 14 SE Ne 15 0 1 1 PPPOE 15 3 12 4 17 3 1 3 IFC Wizard ae 20 24 Sace MeD i 29 3 2 2 40 3 2 1 40 3 2 2 Wizard eeeeeee 44 0 7 RE 50 3 3 1 Wizard
77. Help lehu gt Llnsg CV Results Close Staqndby Rumn Tnnls Windo Help start Standby gt Tools Stop Standby stqndby close BidcoreO Instrument Handbook 3 3 3 3 1 Wizard Wizard 1 2 gt Run Run Project Biacore Control software on LOM1 File Edit Wiem Comrmand Fun Tocls Windnw Help TT RUN Sensorgram ry Run Project Projects 5elErE Project Gelect project Development Published ew Folder ls dl Mnwg Tn Fnldgr ET a Inhihltin ssaW He Hark ggt Llnsm Project
78. Lancgl Project name Save Projects Select Project EE Select project Dewelopmert Published BIA Prnlecls gw Froject hinhi hlnig ew Folder EE Mnwe Tn Fnldgr Protein a Inhihiinn assay _ Hep Bark et gt Close BIA Projects BidcoreO Instrument Handbook Projects 5ElErE Wizard x Froect adrministratior Froect Protein WErslnh dewelnprmernt Froject Properties Publshed Wersions Select wizard mmobilization wizard This wizard guides you Gurface Performance Wizard through the steps Im ssay Deweloprment wizard Immnhllizing ligard on the Enheertraton aha ss WIgarH ssnr Chip surfac ac Megt Clnse Immobilization Wizard Next gt 5elErt TEmnnlate 1 Ewisting template Open ternplate i this project Modified Ho templates awallable Hglp Hack ggt Close New template Next gt Tnnnmobilizatinn General Settings x Ferform mmobilization In Surface Frep Hum settings Nurmber of inections 3 Hun Dacumsntalion Hglp lt Back ent Llngg Perform immobilization in
79. Position Miin Vnl tul Content 1 175 TE 1 14 5 InsE gtr C1 14 5 IinLlsg HGTTTdJTT Help gnu Back agt Llnse Next gt Next gt Start Start Biacore Q Instrument Handbook 77 avre Wizard Template ns aw Ii Cieweloprment EE Eb Filg hame assall lt Mu ahwnrk F ave as uh Wizard template blwy lt Cancel Development C BIA Projects Project Concentration Analysis Development Gare Wizard Results ns awe I Developmert File rame Mu Netwark P ave as upe Results Fbl aic Save Biacore Q Instrument Handbook Loncentration Analysis Results Sustem infarmalian Eyent log Software Biacare Q Control Software wersion 3 0 Time Event Duration lt 22 32 42 Run start Instrument id 3003 22 32 42 Run pre
80. cT lt 5 ample Fositior Ha kx olume 5H Corntact Time I 5 Cornsumptior l Ore 5 Start Injection 1ug ml 10ug ml RU 19000 188nn 18600 RS 15400 1 1820n 18000 17800 17Bnn 17400 SD Time 10 30 2 Command gt Inject 1 Biacore Q Instrument Handbook 43 Tue 30
81. f cucles lt Help ack ent Close Wizard Run sensor chip conditioning No of injection 0 Run flow system conditioning Next gt BidcoreO Instrument Handbook 47 a Gurface Performance User Information Hehngr serid Hmnistralnr Help lt Hack ent gt Llnsg Next gt Gurface Ferformance Rack Fositions Foamon nth Cm TD 1HmM glycinarpH1 5 IMlcHn lt REAG_B LL U1 OUOKOKAKOK Maka Sure that the correct rack g are installed in the autosampler when nu start the ruh _ Hep _ Menu lt Back Net gt
82. he run with a 5erisnr Chip Sustem check The nrncedurg starts Sustem Check hr rirmirg the li uld hardling snstem arr Biacore Q Instrument Handbook Continue Gystem Check Place Thermo rack F in rack base 2 right and Tm Btest soluton im position Fas Three empty ials In the positions RET RE ES Flacg a new buffer corntairer in the hulfer cormpartmernt an insert both imlgt tuhes into the container ahd place an Enhu hntlle under the waste outlet Laricil Start 1 System Check Gystem Check Tn gwalualg the Sustem Check click the Ewalyate httnm Evaluate 7 J HI QO NX 0 Biacore Q Instrument Handbook Biacore Q System Check arTg Created By Biacore Control nftwyarg WeTSlOHI 3 Instrlmeti HH3 Configuration IF FRun Date 2 7 Evaluation Date 5 Dw 2 TmanlysIS tM 2 TO File naine MMTITLED RESULTS FCr Reshnnse RUIr Lnnlh dd 1 Direct IIECT gg5h5 1 Clip Fc 1 Wl CID Fc amp Clip Fc Cp Fc 4 1 EB Refractometer testr 15 UIEFOSE RY Baselimne lewel RU Fc Tt gg5H5 Fc 1T 1337 FH Fr
83. ilization Development Dont Sqve ave Wizard Results hs Ee Mn Lnmh uler ail FlB name Sawe as ME Results Fr lt Carcel 2 BidcoreO Instrument Handbook Save Development C BIA Projects Project Immobilization Development 1 Immabilization Results EE x rmrnobilization Farfarmedim Gukace Frep Flow cell Nnresul arallahle Inr Surface Prep Help Hunhin Imrmobilization Time since start DD OD AS Wizard Ctrl Break BidcoreO Instrument Handbook 39 iTmmmmnbilizatign Results 0 rmrnobilizatior Farfarmedin Suface Frep Flow cell 1 Ho resullt ralahle for Surface Prep Empty the waste beaker if necessary Hun Surface Prep wash from the Working tools menu i Help gnu Llnse Close
84. inn Analysis Add Sample 0g Sample ahlg Fos sample Amount Dilution ReplicatEss Id Factor _ 1 1 1 heasurerment in progress TO Cah add unur samples after 1 3 Iiules FcEl Stqndby Next Continue Standby BidcoreO Instrument Handbook iConcentration Analysis Delete Sample Xl Sample ahle Continue Cancel Delete A This wall delete sample nn 1 STO fram the rur Cahnce OK Concentration Analysis Change Run Order IE EA Sample table Rack Sample Amount Dilution Position Id g Factor 9 69
85. nce Logs and Alerts 4 Replicator Supports File replication in a domain Hb Shared Folders er Es re PrevenedTron makifd ac 3 Device Manager BIAadministrator BIACORE Saftware Administrator Local Users and Groups 4 BIAdeyelaper BIACORE Method Deyelaper Users Eauser BIACORE Routine User aE Storage Disk Management Disk Defraqmenter Loaical Drives Remowable Storage Serwices an Applications 7 1 2 Project BIAadministrator BIAdeveloper Project Project BIAqdministrator BIAdeveloper development project BIAuser published project Projects Mew Project x Project name assay ahorLresgipharn 1 Fasswinrd nr nuhlished project Fasswnr Lnnlirm password EI l Fassword Coir password aw Carcel mm mi gm Hglp Frint Biacore Q Instrument Handbook 95 Development Project Project
86. paration start Fiun information User id administrator Froject Frotein Wersior dewelopment Start tme 2003 11 421 22 32 42 End time Help Pause Menu gt Running preparation Estimated total time left Unknawn End Hun P60 iLoncentration Analysis Results posssssssssssssssssssssses 1 FunLog Calibration Samples Trend Plot Notebook Secsssesesssscsosreer los System information Eyent log Software Biacore Control Software wersion 3 0 Time Event puration lt 18 09 42 Run start 18 09 42 Run preparation start 18 59 49 Run preparation end Fiun information 18 59 49 Calibration start Instrument id 3003 User id dministrator 21 49 50 Calibration end 21 49 50 Analysis start Proiect Protein A 22 38 30 Analysis end Version development 22 38 30 Run end Start time 2003 1 23 18 09 42 End time 20H3 1 23 22 38 3D Pauise Run Log system information
87. r wizard rmobilizatior Manng bo nn includg wizard when publishing EEE IgEHEH IgETgInT i Fi kodiied Protein a bl 1 Carncel Project Wizord OK iPrnjers Puhlish New Yersion 3 Hangral Project a em wersior 1 Fublished hr Narin Effectiwe date D5 lt Hgasnr for Hay 2005 HE WES a 2 Termplates to p 15 1E Wizard 23 ee Immnahiliratid 2 Dn Surfaca Pear FToday 5y22y20n5 a Concentration Analysis elect Template Hglp aw Earncel Effective date Save Biacore Q Instrument Handbook 99 a Biacore 8 Pe The hew wersioh will be used For rnuhne analyses with the published project TE pnLl SMTB WU warnt to publish a mew yrsI ft of this prolect Yes Projects Published ersions _ Assanll ET SO Version Status rive IPublishe Date Wizard Reason foT new Yersion Wi Fublsh New Version Hep Pmt _ ese Close Select Wizard Close
88. roxysuccinimide 1M ethqnolamine hydrochloride pH 8 5 EDC NHS 10 ml MilliQ 200ul 0 5 ml 7mm 20C 1 4C 200 ul 5 200ug ml 10mM pH 0 5 2 pH 18 20 pH 10mM pH3 5
89. rument Handbook 75 iLoncentration Analysis User Information eneral User id Administrator Flow cell ee gt Max no of samples In this rur 2 Notebook Help lt Back Next gt Close Flow cell Max no of sqmples in this run Notebook Next gt eh Concentration Analysis samples Pos Sample Amount Volume Dilutionn Replicates a ra i a TE 2 ediums 3 Hep Eositering lt Back New gt gee Sqmple Table Sample Id Amount 9 Volume ml DilutionFactor Dilution Next gt BidcoreO Instrument Handbook
90. se Calibration Cycle Conc ug ml Calibration Solution Resp RU Data Quqlity Calc Conc CV CV lt Exclude x x Exclude Include Include Biacore Q Instrument Handbook EE bt Results mple Amount Resp Data 8 uality shTElE Sal Sal sgmle 1 sgmle 1 samhle 1 salE 1 salE 1 Hl a P saIThle Esclude sample Help Bae Sample E72 Edit Enu Calt Threshold Calc DTE mount mm mgimb
91. t Wizard Ctrl Break BidcoreO Instrument Handbook 27 ers castrelsae a Immobilization Results Inmnhilzatinn Lewel d Stqndby Close BidcoreO Instrument Handbook Biacore Q Control Software on CUNM1 immob blr Sensorgram EE X kr File Edt View Command Run Toos Window Help Cycle 1 Curve Sensorgram Fc 2 lImmobilization 3BHI 34000 32000 3DO00 280DO0 2 Response 24000 22000 20000 18000 1B000 14000 n 200 400 BI il 1000 1200 1400 1600 1800 2000 Time S Fc PHHG Time bsHesn Slope Baseline HalHesn Id 2 immob 2456 5 17122 9 D0 Yes 0 Baseline 2 immob 1218 5 19852 4 23 30 No 2729 5 Activation 2 mmob 1827 5 19770 1 0 10 No 2647 2 Immobilization Leyel F Sensorgram Fc 2 Infg Time 1857 5s Hesponse 13767 3 RU Timestamp D4 01 21 12 3B 1D Set Temperature 25 0 C Flow 0 plfzmin Flow Cell Temperature 25
92. t gt Notebook BidcoreO Instrument Handbook 25 1 Immabilization Rark Positions Mig position min cahacity 110 pl 11H Frotein 110 Etharnlarming 5Hml Na H MICRO lt REAG_C lt MICRO lt 0000000 nn SO OCCIL CIAL CT OOOOOO OO OOOOOOGO LUJUAUACAKAK UUUOUDCDCDC A IC UI UL LUJCJCACAKAKA UCOM CCAC LDCAL COAT OCIC LEDCAL CIT CCOCONC COC MAC COOCOOCOCOCOCOC DDO DOD Mk sre that the correct rack s are installed in the aitosampler when wo start the un _ Hep _Menu lt Back Next Llase Min Vol pl Min Vol ul Tmmabilizaktion Prepare Run P reparation for immobilization 1 Make sure the correct sensor chip dncked 2 dd reagqents to the folloing postions RERG CU Treadent rack hnsrtinn Fosition
93. t poirt Hgsnnnsg Help enu lt Back Mewl gt Close BidcoreO Instrument Handbook Calibration curve Caqlibration curve Analyte name Molecular weight Fitting function Run order Calibration solution Reporting point DID Calibration points Coalibration solution Replicate 1 1 1 1 Replicgte 1 Concentration 10 8 6 4 2 1 0 10 8 6 4 2 Next gt Calibration parameters Menu Repeat calibration
94. thylenediamine 1M 30min Ethqnol 100 30min Methanol 100 30min 3 Methylqminopropylamine 1M 30min Tetrahydrofuran THF 100 VitaminB2 Surfqce Prep Dock Prime BidcoreO Instrument Handbook Surface Prep Dock Surface Prep Dock Surface Prep 296 Biacore Q Instrument Handbook 31 i
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