Home

Nikon E200 Manual

Contents

1. anevepyonoinon OTPEWTE O 2 M kpook n o ano ano va vep OTPEWTE O OKOUNIOTE Eva navi TOU Av Nik
2. open AHEAEIG Toy o nyiov nou On HEE HE OSION AUTO Greek 40 Safety Precautions ava Tn TIG KAI OXETIKEG va XEIPIOTE TE and onoia Eppnveia A B oAoy k g
3. XEIPIOTEITE 5 ZN caution gt 1 ano TOU n n apaipeon va
4. agaipeite don wpa 30 PPOVTIOTE va va EXEI IAKONEI 30 don wpa 30 e NTNTIKEG VEOTI va 6 En kiv uvo
5. e TOU e and e A EXEI and EENG O KATA TIG NEPIOXEG KATA PWTIONO e MEPIOXEG XOUV
6. va H and AC e am TO NpoBelTe Nikon and AC 68 41 Greek Safety Precautions NnrorrnonorzH 1 MpoopiZopevn To
7. OTEPEWHEVWV NETA I NEVOU TOU 2 H and TO EI ONOIMOTE Nikon 3 H oniogio nivakida
8. Dutch 24 Safety Precautions Betekenis van de symbolen op het instrument De symbolen op het instrument maken u attent dat u tijdens gebruik altijd de nodige voorzichtigheid in acht moet nemen Raadpleeg altijd de handleiding en lees de betreffende aanwijzingen voor u een onderdeel monteert of demonteert waarop het symbool is aangebracht Symbool Betekenis Let op Gevaar voor biologische besmetting Dit symboollabel dat op de brug is bevestigd maakt u attent op het volgende WAARSCHUWING Door contact tussen het preparaat en de microscoop kan gevaar voor biologische besmetting ontstaan e Raak het verontreinigde deel niet met blote handen aan om verspreiding van de biologische besmetting te voorkomen e Reinig het verontreinigde deel volgens de standaardprocedure van uw laboratorium A Pas op heet Dit symbool vindt u op de lensunit de lamp zit onder de lensunit en maakt u attent op het volgende e De lamp en de omgeving van de lamp inclusief de lensunit worden erg heet wanneer de lamp ingeschakeld is en blijven dat nog enige tijd nadat de lamp uitgeschakeld is e Gevaar voor brandwonden Raak de lamp en de omgeving ervan niet aan wanneer de lamp brandt of wanneer deze net uitgeschakeld is e Controleer of de lamp en de omgeving ervan voldoende afgekoeld is voordat u probeert de lamp te vervangen Let op Dit symbool vindt u op de wisselstroomsluiting en maakt u attent op het vol
9. Connecting the Power Cord Turn off the power switch of the microscope turn to O Connect one end socket of the supplied power cord to the AC inlet on the rear of the microscope Connect the other end plug to an AC line receptacle with the ground conductor earth conductor Make sure that the power cord is securely connected e Note that the microscope should be installed near the AC line receptacle and the AC line receptacle should be placed within your reach e Use the power cord provided The use of other cords may damage the instrument or cause a fire hazard e If using an extension cord use only a cord that includes a protective earth PE wire This completes the assembly of the microscope standard set 69 Chapter 6 Assembly 2 Assembly of Additional Components EM Assembly of Additional Components 1 Condenser The condenser is attached to the microscope before shipment When removing or replacing the condenser follow the procedure below 1 Lower the condenser holder to its full limit by rotating the condenser focus knob Condenser focus knob 2 Screw in the auxiliary lens to the bottom of the condenser Condenser holder 3 Condenser 4 Position the condenser with its nameplate facing front Tighten the condenser clamp Condenser clamp screw screw located to the left 3 Slide the condenser into the condenser holder 5 Raise the condenser holder to its full limit by
10. ano diodo AC OTI AM TIG H uikpockoniwv KAI nou e avaypa nevn gt Nikon e Av avaypap pevn ano AC diag gt TN 0 68 100 110 120 V H unopei AC 100 V 110 Vn 120 V nivakida TEXVIKWV
11. 220 230 240 V H unopei va AC 220 V 230 Vn 240 V 4 H nupkayi o 85 PE e 6V 20W PHILIPS 7388 OSRAM HLX64250 6V 30W PHILIPS 5761 e 250 V 1A 2x pivi 5x20 Greek 42 Safety Precautions N nrorrnonorHzH 5 O
12. Handle the lamp gently Shocks and vibrations will damage the lamp or reduce its service life When changing the lamp be sure that the contact is not damaged If the contact is damaged the lamp may not light up or may overheat Insert the lamp s contact pins fully into the socket holes If the pins are loose the lamp could come off or result in a contact failure which will cause overheating or smoke Also make sure that the field lens unit is securely attached Do not break the used lamps instead dispose of them as special industrial waste or according to the laws applicable to your municipal waste system 44 Notes on Handling the System 4 Refocusing When changing specimens using the refocusing mechanism gently lower the stage by hand taking care not to hit the field lens with the condenser holder p 60 5 Focus Knobs Do not turn the right and left focus knobs simultaneously in opposite directions Do not turn the coarse focus knob any further after the stage has been moved up or down to its limit These operations will damage the focusing mechanism The coarse focus knob has a protection device The knob turns freely for a while after it has reached its upper limit 6 Oil Immersion Observation p y Make sure not to hit the field lens Do not turn the knobs in opposite directions Use only a minimum quantity of oil If too much oil is applied surplus oil could flow out t
13. An Angabe der Eingangsspannung 4 Vorgeschriebenen Lampen Sicherungen und Netzkabel verwenden Verwenden Sie die vorgeschriebenen Lampen und Sicherungen und das mitgelieferte Netzkabel Bei Verwendung falscher Lampen Sicherungen und Netzkabel kann es zur Besch digung des Instruments oder Feuerentwicklung kommen Beziehen Sie sich hinsichtlich des Netzkabels auch auf Seite 85 Bei Verwendung eines Verl ngerungskabels nur Kabel mit Schutzerdung verwenden Vorgeschriebene Lampe Halogenlampe 6 V 20 W PHILIPS 7388 oder OSRAM HLX64250 oder Halogenlampe 6 V 30 W PHILIPS 5761 Vorgeschriebene Sicherung 250 V 1 A tr ge Sicherung 5x20 Miniatursicherungen x 2 German 10 Safety Precautions FR WARNUNG 5 Hitzeentwicklung der Lichtquelle Die Lampe wird im Betrieb sehr hei Die Kollektivlinseneinheit nicht entfernen w hrend die Lampe eingeschaltet ist und vor deren Ber hrung darauf achten dass diese fur mindestens 30 Minuten ausgeschaltet ist e Achten Sie bei Ersatz der Lampe darauf dass diese zum Anfassen ausreichend weit abgek hlt ist die Beleuchtung sollte f r mindestens 30 Minuten ausgeschaltet sein e Die Lampe nicht ber hren wenn diese eingeschaltet ist oder f r mindestens 30 Minuten ausgeschaltet ist da es sonst zu Verbrennungen kommen kann e Niemals Stoff Papier oder brennbare fl chtige Substanzen wie Benzin Petroleumbenzin Aceton Verd nner oder Alkohol in die N he einer heiBen
14. Replacing the Fuse I DIDI 74 Chapter 7 Optical Characteristics 4 ERR REE ER ERE nenn nun nun nen nun nun nenn 75 Contents Chapter 8 Troubleshooting Tables Chapter 9 Care and Maintenance Chapter 10 Technical Specifications 44 ann nun nun nun nennen nenn nun eneen Safety Precautions Safety Precautions Warning Caution symbols used in this manual OLI TIROLO LITI LIO LI TIT II CITI LO LO III III III III IO Although Nikon products are designed to provide you with the utmost safety during use incorrect usage or disregard of the instructions can cause personal injury or property damage and will lead to the forfeiture of all claims against warranty For your safety read the instruction manual carefully and thoroughly before using the instrument Do not discard this manual but keep it near the product for easy reference In this manual safety instructions are indicated with the symbols shown below Be sure to follow the instructions indicated with these symbols to ensure correct and safe operation Symbol Meaning ZN warnine Disregarding instructions marked with this symbol may lead to death or serious injury ZN caution De instructions marked with this symbol may lead to injury or property English 4 Safety Precautions Meaning of symbols used on the equipment The symbol appearing on the product indicates the n
15. Cambiare l impostazione della tensione in ingresso vedere a p 68 21 Italian Safety Precautions ZN avvertenza 1 Uso specifico dell apparecchio Il microscopio stato progettato soprattutto per l uso in osservazioni microscopiche di cellule e tessuti fissati su vetrini tramite l ausilio di illuminazione diascopica trasmessa ed episcopica riflessa Esso stato progettato per gli scopi principali della sperimentazione e dell osservazione negli ospedali o in altri laboratori di cellule e tessuti che rientrano nei campi della patologia e della citologia 2 Nonsmontare il microscopio Lo smontaggio pu causare malfunzionamenti e o folgorazioni e rende nulli tutti i reclami relativi alla garanzia Non smontare nessuna parte diversa da quelle descritte in questo manuale Se si riscontra un problema con il microscopio segnalarlo al rappresentante Nikon pi vicino 3 Controllare la tensione in ingresso La tensione in ingresso indicata sul retro del microscopio in due punti sulla targhetta con la sigla e sopra l ingresso in c a Controllare che le indicazioni della tensione in ingresso corrispondano alla tensione disponibile nella propria area geografica In caso contrario seguire una delle istruzioni indicate qui sotto L uso del microscopio con tensione in ingresso diversa dalle indicazioni causa sovracorrente e surriscaldamento che possono a loro volta provocare incendi o gravi danni al microscopio s
16. Pour un microscope plaque du fabricant indiquant ad 123 220 230 240 V m La tension est reglable 220 V 230 V ou 240 V CA 4 Emploi de l ampoule du fusible et du cordon secteur sp cifi s Utilisez l ampoule et le fusible sp cifi s Utilisez le cordon secteur fourni L emploi d une ampoule d un fusible ou d un cordon d alimentation incorrects peut endommager l instrument ou provoquer un incendie Rapportez vous aussi la page 85 pour le cordon secteur Si vous utilisez une rallonge utilisez seulement un cordon avec fil de mise la terre de protection PE Ampoule sp cifi e Ampoule halog ne 6 V 20 W PHILIPS 7388 ou OSRAM HLX64250 ou Ampoule halog ne 6 V 30 W PHILIPS 5761 e Fusible sp cifi 250 V 1 A type action retard e coupure basse fusible miniature 5x20 x2 French 14 Safety Precautions ZN AVERTISSEMENT 5 Chaleur mise par la source lumineuse La lampe devient tr s chaude pendant l emploi Ne retirez pas la lentille de champ quand la lampe est allum e et v rifiez que la lampe est teinte depuis 30 minutes avant de la toucher e Au remplacement de l ampoule v rifiez que la lampe est suffisamment refroidie pour la toucher la lumi re doit tre teinte depuis au moins 30 minutes e Ne touchez pas la lampe quand elle est allum e ou avant qu elle ne soit rest e teinte pendant 30 minutes sinon des br lures sont possibles e N approchez jamais du tissu
17. e AVVERTENZA Il contatto tra il campione e il microscopio pu causare pericoli biologici e Per evitare la contaminazione con rischio biologico non toccare la porzione contaminata con le mani nude e Decontaminare la parte contaminata in base alle procedure standard del proprio laboratorio A Attenzione al calore Questa etichetta simbolica attaccata accanto all unita lente di campo la lampada installata sotto all unit lente di campo serve per ricordare quanto segue e La lampada e le aree circostanti compresa l unit lente di campo diventano molto calde durante e immediatamente dopo l accensione e Rischio di scottature Non toccare la lampada o le aree circostanti durante e immediatamente dopo l accensione e Prima di tentare di sostituire la lampada verificare che la lampada e le aree circostanti si siano raffreddate a sufficienza Attenzione Questa etichetta simbolica attaccata accanto all ingresso in c a serve per ricordare quanto segue e Prima di accendere il microscopio controllare la tensione in ingresso La tensione in ingresso indicata sulla targhetta con la sigla e sopra l ingresso in c a e Se la tensione in ingresso indicata diversa dal livello di tensione locale non accendere il microscopio Invece eseguire le azioni seguenti Tensione diversa sulla targhetta con la sigla Contattare il rappresentante Nikon pi vicino Tensione diversa sopra l ingresso in c a
18. ksesi tartunnan ja varo ettet kosketa n ytett Mik li n yte joutuu kosketuksiin mikroskoopin kanssa dekontaminoi kontaminoitunut alue laboratorion normaalien menettelytapojen mukaisesti r NNvarotormeT 1 Kytke virtakytkin pois p lt ennen kuin kokoat mikroskoopin tai vaihdat lampun tai sulakkeen ja laita verkkojohto paikoilleen tai irrota se K nn virtakytkin pois p lt ennen kuin liit t tai irrotat verkkojohdon s hk iskun tai tulipalon est miseksi K nn virtakytkin pois p lt ja irrota verkkojohto myds ennen kuin kokoat mikroskoopin ja ennen kuin vaihdat lampun tai sulakkeen Virran pois kytkemiseksi aseta virtakytkin asentoon O 2 Pid mikroskooppi vapaana kosteudesta ja vieraista esineist Pid mikroskooppi vapaana kosteudesta est ksesi oikosulun joka voi johtaa ylikuumenemiseen tai muihin toimintah iri ihin Mik li vahingossa l ikyt t vett mikroskoopille k nn v litt m sti virtakytkin pois p lt k nn asentoon O ja irrota verkkojohto Pyyhi sitten vesi pois kuivalla liinalla Oikosulku voi tapahtua my s jos mikroskoopin sis lle j vieras esine Jos mikroskooppiin on joutunut vieras esine tai vett l k yt mikroskooppia ja ota yhteys l himp n Nikonin edustajaan 3 Objektip yd n teline Objektip yd n teline ty ntyy esiin k yt n aikana Ole varovainen ettet osu telineeseen k sivarrella tai muulla ruumiin osalla kun k sittelet mikroskoo
19. s 85 verkkojohdosta Jos k yt t jatkojohtoa k yt vain johtoa jossa on suojamaadoitusjohdin e M ritelty lamppu Halogeenilamppu 6V 20W PHILIPS 7388 tai OSRAM HLX64250 tai Halogeenilamppu 6V 30W PHILIPS 5761 e M ritelty sulake 250 V 1A tyyppi aikaviive alhainen virrankatkaisu 5x20 pienoissulaketta x 2 Finnish 38 Safety Precautions AN varorrus 5 Valol hteen aiheuttama kuumuus Lamppu kuumenee k yt n aikana l irrota kentt linssiyksikk kun lamppu on p ll ja varmista ett lamppu on ollut pois p lt 30 minuuttia ennen kuin kosket sit e Kun vaihdat lamppua varmista ett lamppu on riitt v n j htynyt jotta sit voi koskettaa valon pit olla pois p lt ainakin 30 minuuttia e l kosketa lamppua kun se on p ll tai ennen kuin se on ollut pois p lt 30 minuuttia sill se voi johtaa palovammoihin e l tuo kangasta paperia tai herk sti syttyv ainetta kuten bensiini ljy asetonia tinneri tai alkoholia kuumanlampun l helle sill se voi aiheuttaa tulipalon 6 Vaarallinen n yte T m mikroskooppi on tarkoitettu p asiassa mikroskoopilla teht vien solujen ja kudoksen havaintojen tekoon n ytelevyilt Kun k sittelet n ytett tarkista onko se vaarallinen K sittele vaarallisia n ytteit laboratorion normaalien menettelytapojen mukaisesti Mik li n yte on luonteeltaan tarttuvaa k yt kumihansikkaita v ltt
20. using the voltage selector P 68 83 Care and Maintenance Cleaning the Lenses e Dust is best removed using a soft brush or gauze e More persistent dirt such as fingerprints grease and oil may be removed with lens tissue or soft cotton gauze lightly moistened with absolute alcohol anhydrous ethyl alcohol or methyl alcohol do not use rubbing alcohol e To clean immersion oil off the oil immersion type objective use lens tissue soft cotton or gauze lightly moistened with petroleum benzine If petroleum benzine is not available use methyl alcohol In this case you need to wipe three or four times because the detergency of the methyl alcohol is somewhat weak e Absolute alcohol and petroleum benzine are quite inflammable Take great care when handling them and when turning the power switch on and off Be very careful with fire Cleaning the Microscope We recommend that you use a silicon cloth to clean the microscope e For persistent dirt dampen a piece of gauze with neutral detergent and wipe lightly e Using organic solvent could result in discoloration of the plastic parts EN Disinfecting the Microscope e We recommend that you use 70 medical alcohol for normal disinfection of the microscope e In case of contact of a sample to the microscope determine whether the sample is hazardous If the sample is hazardous follow the standard procedure of your laboratory e Using organic solven
21. Lampe bringen da dies zur Feuerentwicklung f hren k nnte 6 Gef hrliche Proben Dieses Mikroskop ist haupts chlich f r mikroskopische Beobachtungen von auf Objekttr gern fixierten Zellen und Gewebeproben gedacht Pr fen Sie beim Umgang mit den Proben ob diese gef hrlich sind Gef hrliche Proben entsprechend der Standardprozeduren in Ihrem Labor behandeln Wenn es sich bei der Probe um infekti ses Material handelt Gummihandschuhe tragen um Infektionen zu vermeiden und sorgf ltig darauf achten die Probe nicht zu ber hren Wenn Proben mit dem Mikroskop in Ber hrung kommen die kontaminierten Abschnitte entsprechend der Standardprozedur in Inrem Labor entseuchen vorsicut 1 Vor Zusammenbau Ersatz von Lampe oder Sicherung sowie AnschlieBen oder L sen des Netzkabels den Netzschalter ausschalten Vor AnschlieBen oder L sen des Netzkabels den Netzschalter ausschalten um elektrische Schl ge und Feuerentwicklung zu vermeiden Vor Zusammenbau des Mikroskops oder Ersatz von Lampe oder Sicherung ebenfalls den Netzschalter ausschalten und den Stecker aus der Steckdose ziehen Den Netzschalter in die O Stellung dr cken um den Strom auszuschalten 2 Das Mikroskop vor Feuchtigkeit und Fremdk rpern sch tzen Sch tzen Sie das Mikroskop vor Feuchtigkeit um Kurzschl sse zu vermeiden die durch Uberhitzung oder andere St rungen hervorgerufen werden k nnten Wenn Wasser auf dem Mikroskop versch ttet wird sofort
22. NES gt I th tive P objective especially 40x objective Clean the objectiver Aperture diaphragm and field diaphragm gt ly P opened or closed too far Close Or pen properly Image dark on one side Causes Corrective Measures Revolving nosepiece not in click stop gt a Revolve to click stop position position Specimen rises from stage surface gt Stabilize it using the holder Image shifts during focus Causes Corrective Measures Revolving nosepiece not in click stop de Revolve to click stop position position Specimen rises from stage surface Stabilize it using the holder Field diaphragm not centered if the microscope is equipped with a field diaphragm Center the field diaphragm P 57 Field lens unit not installed correctly Install correctly P 72 79 Chapter 8 Troubleshooting Tables 1 Optical Image tinged yellow Causes Corrective Measures Blue filter not used Use blue filter P 70 Adjust the voltage by rotating the brightness control dial 54 Image too bright Causes Corrective Measures Adjust the voltage by rotating the Lamp voltage teo high gt brightness control dial P 54 Insufficient brightness Causes Corrective Measures Adjust the voltage by rotating the Lamp Voltage top I0w brightness control dial P 54 Aperture diaphragm closed too far Open properly P 59 vv If the microscope is equippe
23. The objective s numerical aperture is indicated on the side of the objective 40x 0 65 magnifying power 40x numerical aperture 0 65 e To observe the diaphragm image remove the eyepiece and look down the open tube The eyepiece is fastened to the eyepiece tube Loosen the screw before removing the eyepiece e The figures on the condenser show the approximate positions of the aperture diaphragm lever corresponding to each objective magnification When the aperture diaphragm lever is moved to that position the size of the aperture diaphragm will be 70 80 of the objective s numerical aperture Each time you switch the objective align the aperture diaphragm lever to the same figure as the magnifying power of the objective to get a good image with sufficient contrast N Nikon Corresponding objective magnification 7 E Plon 3 40 x 0 65 Aperture diaphragm f 0 17 wo 0 65 lever 100X 40X 20X 10x 4x ui vum Eco Magnifying Power 40x Numerical Aperture 0 65 af 9 BX Aperture diaphragm image The appropriate size of the aperture diaphragm 59 Chapter 4 Microscopy Detailed Procedure The next procedure 10 is for the microscopes with field diaphragms If the microscope does not have a field diaphragm skip to procedure 11 1 Field Diaphragm Adjustment for microscopes with the field diaphragm The field diaphragm is used to control the specimen s illuminated area relative
24. all utente il massimo della sicurezza durante l uso l utilizzo non corretto o la mancata osservanza delle istruzioni possono causare ferite personali o danni materiali e rendono nulli tutti i reclami relativi alla garanzia Per la sicurezza personale prima di utilizzare l apparecchio leggere con attenzione e completamente il manuale di istruzioni Non gettare questo manuale ma tenerlo vicino al prodotto per comodit di riferimento In questo manuale le istruzioni per la sicurezza sono indicate tramite i simboli riportati qui sotto Si raccomanda di seguire le istruzioni indicate accanto a questi simboli per garantire un uso corretto e sicuro Simbolo Significato AN avvertenza La mancata osservanza delle istruzioni contrassegnate da questo simbolo pu portare alla morte o a gravi ferite La mancata osservanza delle istruzioni contrassegnate da questo simbolo pu portare a ZN arrenzrone i i FRE ferite o a danni materiali Italian 20 Safety Precautions Significato dei simboli utilizzati sull apparecchio I simboli che compaiono sull apparecchio indicano il bisogno di attenzione nei vari momenti dell uso Prima di manipolare qualsiasi parte sulla quale attaccato uno dei simboli consultare sempre il manuale di istruzioni e leggere le istruzioni relative Simbolo Significato A Attenzione Pericoli biologici Questa etichetta simbolica attaccata sul supporto serve per ricordare quanto segue
25. and the filter holder gt 6 Condenser Clamp Screw 16 Condenser focus knob e rn Nameplate Mme SAA 19 Coarse focus knob torgue adjustment ring 18 Coarse focus Knob 22 AC Inlet 17 Fine focus knob 21 Voltage selector Fuse holder 49 3 A Quick Microscopic Procedure O For microscopes without a field diaphragm Widen or narrow to i merge the O C J viewfields into y BE q E DO Here s the power switch N Turn on the lamp amp CC gt and adjust Viewfields lt interpupillary distance A 8 Es RS Adjust x m brightness too Y Adjust the Focus with 10x diopter objective O O M 40x NH Coverglass up GN TR Es 7 Focus with this SE i Match the bottom edge of the knob dS diopter ring with gt W the engraved base 0 line N Use this knob for focusing 90 Chapter 3 A Quick Microscopic Procedure Turn off the power Wait till the microscope cools down before storing D FAO SU Sa l Check the 7 magnifying power gt 2 Move the lever to the magnifying power of the objective Then focus ig with this ring Magnify the image Use your left and observe eye OX Repeat E O to O twice 5 And focus with this ring Use your right eye 10 X 51 Chapter 3 A Quick Microscopic Procedure For microscopes equipped with the field d
26. back and lower the condenser slightly Add a drop of oil on the front of the condenser from the long hole on the stage Bring the specimen back over the condenser and slowly raise the condenser Objective e Rotate the revolving nosepiece to move the PA c objective out of position Add a drop of oil to the specimen Slowly rotate the revolving nosepiece to bring the objective back into position O Eliminate Air Bubbles Make sure that air bubbles are not trapped during oil application Air bubbles degrade the image To see if any air bubbles are trapped in the oil remove one eyepiece and fully open the aperture diaphragm and field diaphragm if the microscope has the field diaphragm Look into the eyepiece tube and check the objective pupil a bright round part If you cannot see it well replace one of the eyepieces with the adapter and the centering telescope both optional and look through the eyepieces of the centering telescope while rotating the eyepiece part of the centering telescope Do any of the following to eliminate air bubbles e Rotate the revolving nosepiece to move the objective back and forth e Gently rotate the condenser focus knob to move the condenser up and down e Add another drop of oil e Wipe off the oil and apply again 62 Chapter 5 Miscellaneous Operations 1 Oil Immersion Observation O Handling of the Immersion Oil Use a minimum quantity of oil If too much oil is applied surpl
27. back in place after replacing the lamp Never turn on the lamp without the field lens unit e Do not touch the glass part of the lamp using your bare hands Wear gloves or use a cloth when handling the lamp to protect the surface from fingerprints Wipe off any fingerprints or stains using a clean cloth moistened with alcohol Fingerprints will etch into the hot surface of the lamp and reduce the brightness damage the lamp or reduce its service life e Handle the lamp gently Shocks and vibrations will damage the lamp or reduce its service life e Do not break the used lamps instead dispose of them as special industrial waste or according to the laws applicable to your municipal waste system 72 Chapter 6 Assembly 1 2 3 4 5 6 7 3 Replacement of Consumable Materials Turn off the power switch turn to O and unplug the power cord Wait about 30 minutes until the lamp and its surroundings are cool enough to touch Hold the field lens unit at the vertical grooves on both sides and pull it toward you to remove it Remove the old lamp Hold a new lamp wearing gloves or using a cloth Insert the lamp s contact pins fully into the socket holes straight and securely Put the field lens unit back in place Plug the power cord e Field lens unit 73 Chapter 6 Assembly 3 Replacement of Consumable Materials 2 Replacing the Fuse ZN warnine To avoid ele
28. can be used for winding the power cord around when the microscope is not in use To remove the hangers use a screwdriver Cord hangers 67 Assembly Read the Safety Precautions in this manual before assembling the microscope Be sure to follow the instructions written therein Also make sure that the power switch is off turned to O before assembly to prevent electrical shock Tools Required for Assembly Hexagonal wrench one hexagonal wrench is provided with the microscope flatblade screwdriver 1 Assembly of Standard Set 1 Input Voltage Check The input voltage is indicated in two places at the rear of the microscope on the nameplate and above the AC inlet Confirm that these input voltage indications correspond to the voltage provided in your region If not follow one of the instructions below The use of microscopes with the different input voltage indications will cause overcurrent and overheating which may result in fire or severe damage to the microscope e If the voltage indication on the nameplate differs Do not plug in the microscope Contact your nearest Nikon representative e If the voltage indication above the AC inlet differs Change the input voltage setting before turning on the power switch For the microscope with the nameplate showing 100 110 120 V The voltage can be set to AC 100 V 110 V or 120 V Input Voltage Indications e For the microscope with
29. e Dirt and dust degrade optical performance and are to be avoided e Vibrations in the environment will degrade the image Install the microscope in a location free of vibrations e Install the microscope on a solid table and keep the microscope level e Select a layout that allows easy detachment of the power cord from the AC inlet of this microscope in the event of emergency e This microscope emits a feeble electromagnetic wave Do not place a precision electronic device near the microscope as precision could be degraded Also avoid placing a radio or TV near the microscope as reception of sound and images may be hampered 2 Carrying the Microscope This microscope is a precision instrument Handle it gently Strong shocks and forcible operation will damage the instrument Shocks to the objectives especially could degrade image precision e When carrying the microscope hold it at its upper rear and lower front ends e Do not hold the focus knobs the eyepiece tube or the stage These parts could easily come off and could result in malfunctions 3 Handling the Lamps Do not touch the glass part of the lamp with bare hands Wear gloves or use a cloth when handling the lamp so as not to leave fingerprints on the surface Wipe off any fingerprints or stains using a clean cloth moistened with alcohol Fingerprints will etch into the hot surface of the lamp and reduce the brightness damage the lamp or reduce its service life
30. ensamblado del microscopio y antes del cambio de la l mpara o del fusible Para desconectar la energ a ponga la llave de energ a en posici n O 2 Mantenga el microscopio libre de humedad y materias extra as Mantenga el microscopio libre de humedad para prevenir el cortocircuito que puede causar el recalentamiento u otros desperfectos En el caso de salpicar el agua en el microscopio desconecte inmediatamente la llave de energ a fijando el interruptor en posici n O y desenchufe el cable de energ a Luego limpie el agua con pa o seco El cortocircuito puede producirse al penetrar una materia extra a dentro del microscopio En el caso de entrar el agua o materia extra a dentro del microscopio no utilice el microscopio y tome contacto con el representante Nikon m s pr ximo 3 Cremallera de la platina La cremallera de la platina sobresale durante el uso Cremallera Tome la precauci n de no golpear la cremallera con sus manos u otras partes de su cuerpo durante la manipulaci n del microscopio para evitar las lesiones con el borde de la cremallera 4 Disposici n del microscopio Para evitar el riesgo biol gico el microscopio deber disponerse como equipo contaminado de acuerdo con el procedimiento normal de su laboratorio 19 Spanish Safety Precautions Precauzioni per la sicurezza Simboli di Avvertenza Attenzione utilizzati in questo manuale ns Sebbene i prodotti Nikon siano progettati per garantire
31. field lens eyepiece specimen p gt Corrective Measures If the microscope is equipped with a field diaphragm correct positioning so that the field diaphragm image is focused on the specimen surface P 57 If the microscope is not equipped with a field diaphragm position the condenser slightly lower than the upper limit Open properly P 59 E I Clean the lens P 84 Poor image quality low resolution contrast too low or too high Causes Condenser position too low Cover glass too thick or thin Slide upside down y Corrective Measures If the microscope is equipped with a field diaphragm correct positioning so that the fie ld diaphragm image is focused on the specimen surface P 57 If the microscope is not equipped with a field diaphragm position the condenser slightly lower than the upper limit Use a cover glass of the specified thickness 0 17 mm Turn over the slide so that the cover glass faces up No cover glass attached to the slide No immersion oil used on the front lens of the oil immersion objective Nikon immersion oil is not used for oil immersion observation Air bubbles in immersion oil 78 gt gt gt gt EE Attach a cover glass 0 17 mm thick Apply Nikon immersion oil to the objective P 62 Use Nikon immersion oil P 62 Remove bubbles P 62 Chapter 8 Troubleshooting Tables 1 Optical Immersion oil found on dry type
32. la l mpara deber quedar apagada por lo menos 30 minutos e No toque la l mpara cuando est encendida o hasta que la l mpara haya quedado apagada por 30 minutos ya que de lo contrario puede causar quemaduras e Jam s deber acercarse la ropa papel o sustancias vol tiles inflamables como la gasolina bencina de petr leo acetona solvente o alcohol cerca de la l mpara caliente debido a que puede causar el incendio 6 Muestra peligrosa Este microscopio se utiliza principalmente para la observaci n microsc pica de c lulas y tejidos fijados en el portaobjetos Durante la manipulaci n de una muestra verifique si la muestra es peligrosa Las muestras peligrosas deben manipularse seg n los procedimientos normales de su laboratorio Cuando la muestra sea de una naturaleza infecciosa utilice los guantes de goma para evitar la infecci n y tome las precauciones para no tocar la muestra En el caso de producirse el contacto de la muestra con el microscopio descontamine la porci n contaminada seg n los procedimientos normales de su laboratorio N precauci n 1 Desconecte la energ a antes de ensamblar el microscopio reemplazar la l mpara o fusible y enchufar o desenchufar el cable de energ a Desconecte la llave de energ a antes de enchufar o desenchufar el cable de energ a para prevenir la descarga el ctrica o el incendio Desconecte tambi n la llave de energ a y luego desenchufe el cable de energ a antes del
33. manual sino guardarlo cerca del producto para que pueda consultarse en cualquier momento Las instrucciones de seguridad de este manual est n marcadas con los simbolos que se indican a continuaci n Se ruega respetar las instrucciones indicadas con estos simbolos para asegurar la operaci n correcta y segura Simbolo Significado Al no respetar las instrucciones indicadas con este simbolo puede causar la muerte o ZN avverteNcIA ia A lesiones serias PRECAUCI N Al no respetar las instrucciones indicadas con este simbolo puede causar lesiones o da os materiales Spanish 16 Safety Precautions Significado de los simbolos utilizados en este equipo Los simbolos que aparecen en el producto indican la necesidad de tomar las debidas precauciones en todo momento durante su uso Consulte siempre el manual de instrucciones y lea las indicaciones antes de manipular cualquier parte donde est n adheridos los simbolos Simbolo Significado A Precauci n contra el peligro biol gico El r tulo con este simbolo adherido en el soporte le recuerda lo siguiente e ADVERTENCIA El contacto entre la muestra y el microscopio puede constituir un riesgo biol gico e Para evitar la contaminaci n del peligro biol gico no toque las partes contaminadas con sus manos descubiertas e Descontamine la parte contaminada seg n los procedimientos normales de su laboratorio Precauci n contra el calo
34. ou espere no m nimo 30 minutos caso contr rio pode provocar queimaduras e Nunca aproxime pano papel ou subst ncias vol teis inflam veis como gasolina benzina acetona diluente ou lcool de uma l mpada quente porque pode provocar inc ndios 6 Amostra perigosa O presente microsc pio destina se principalmente observa o microsc pica da c lulas e tecido apostos no slide Ao manusear uma amostra verifique se a referida amostra perigosa Manuseie as amostras perigosas de acordo com o procedimento padr o do laborat rio Se a amostra tiver uma natureza infecciosa calce luvas de borracha para evitar infec es e tenha cuidado para n o tocar na amostra No caso de contacto de uma amostra com o microsc pio descontamine a parte contaminada de acordo com o procedimento padr o do laborat rio r N precau o 1 Desligue a energia antes de montar o microsc pio substituir a l mpada ou o fus vel e de ligar e desligar o cabo el ctrico Desligue o interruptor antes de ligar ou desligar o cabo el ctrico para impedir choques el ctricos ou inc ndios Desligue tamb m o interruptor e depois desligue o cabo el ctrico antes de montar o microsc pio e antes de substituir a l mpada ou o fus vel Para desligar a energia coloque o interruptor emo 2 Mantenha o microsc pio sem humidade e mat rias estranhas Mantenha o microsc pio sem humidade para impedir curto circuitos que podem provocar o sobreaquecimento
35. si maneggia il microscopio fare attenzione a non colpire l intelaiatura con le mani o con altre parti del corpo perch possibile ferirsi a causa di un bordo dell intelaiatura Intelaiatura 4 Smaltimento del microscopio Per evitare rischi biologici smaltire il microscopio come apparecchio contaminato in base alla procedura standard del proprio laboratorio 23 Italian Safety Precautions Veiligheidsmaatregelen In deze handleiding gebruikte symbolen Hoewel de producten van Nikon ontworpen zijn om u de hoogste mate van veiligheid tijdens gebruik te garanderen kan bij onjuist of oneigenlijk gebruik persoonlijk letsel of schade aan het apparaat ontstaan en in dergelijke gevallen zullen alle garantieclaims afgewezen worden Lees voor uw eigen veiligheid aandachtig de handleiding voordat u het instrument gaat gebruiken Bewaar deze handleiding zorgvuldig zodat u deze later in voorkomende gevallen opnieuw kunt nalezen De veiligheidsinstructies in deze handleiding zijn gemarkeerd met onderstaande symbolen Volg de onderstaande aanwijzingen nauwgezet op zodat u het instrument correct en veilig kunt gebruiken Symbool Betekenis NS waarscHuwiInG Waarschuwt voor een potentieel gevaarlijke situatie die de dood of ernstig persoonlijk letsel tot gevolg kan hebben Aver OP Waarschuwt voor een potentieel gevaarlijke situatie die ernstig persoonlijk letsel of schade aan het instrument tot gevolg kan hebben
36. to the microscope s viewfield If it is opened to a larger aperture than necessary extraneous light will enter the viewfield which may reduce the image contrast Every time you change the objective i Corresponding magnifications rotate the field diaphragm ring until objective the diaphragm just leaves the viewfield magnification tox 20X 10x 4x e The figures around the field diaphragm show the approximate positions for field diaphragm ring corresponding to each objective magnification when 10x eyepieces are used e For 100x objectives the field diaphragm cannot be closed enough Replacing a Specimen Using the Refocusing Mechanism Try focusing on the specimen with 40x or higher magnification objective You will find the specimen is brought very near to the objective It will be very difficult to change the specimen without moving the focus knob In a case like this use the refocusing mechanism for easy specimen replacement 1 Use one hand to gently press down the stage 2 While holding the stage at that position change the specimen Gradually release the stage so that it rises slowly The stage will return to the focal position Make sure not to hit the field lens The distance between the front of the objective and the specimen when the specimen is in focus is called the working distance of the objective For details see p 74 When lowering the stage take great care not to hit the field lens with t
37. 1 5 mm 63 2 um 2 8 um 30 mm 10x 100x 0 25 2 mm 10 1 um 1 1 um 7 mm 40x 400x 0 65 0 5 mm 1 2 um 0 4 um 0 65 mm 100x 1000x 1 25 0 2 mm 0 4 um 0 2 um 0 23 mm Microscope Terminology 1 Total Magnification The total magnification of a microscope is the individual magnifying power of the objective multiplied by that of the eyepiece 2 Numerical aperture N A The numerical aperture is an important factor in determining the efficiency of the condenser and objective It is represented by the formula N A nsina where n is the refractive index of the medium air immersion oil etc between the objectivelens and the specimen or condenser and is half of the maximum angle at which light enters or leaves the lens from or to a focused object point on the optical axis The larger the numerical aperture the brighter the image and the higher the resolution 3 Resolving Power The ability of an optical system to discriminate between two discrete objects separated by a minute distance The more minute the distance the higher the resolving power of the optical system In relation to the numerical aperture the resolving power is represented by the following formula 2x Resolving power where is the used wavelength of light The resolving power in the above table is indicated for A 0 55um 4 Working Distance W D The clearance between the front of the objective and the upper surface of
38. 5 over het netsnoer Gebruik wanneer een verlengsnoer wordt toegepast uitsluitend een verlengsnoer met randaarde Voorgeschreven lamp Halogeenlamp 6V 20W PHILIPS 7388 of OSRAM HLX64250 of Halogeenlamp 6V 30W PHILIPS 5761 Voorgeschreven zekering 250 V 1A trage zekering 5x20 minizekeringx2 Dutch 26 Safety Precautions use UWING Warmte van de lichtbron De lamp wordt heet tijdens gebruik Verwijder de lensunit nooit terwijl de lamp ingeschakeld is en raak de lamp pas aan nadat deze gedurende 30 minuten uitgeschakeld is e Zorg er bij het vervangen van de lamp voor dat deze genoeg afgekoeld is zodat u deze kunt aanraken wacht tenminste 30 minuten nadat de lamp uitgeschakeld werd e Raak om brandwonden te voorkomen de lamp niet aan wanneer deze ingeschakeld is of minder dan 30 minuten geleden uitgeschakeld werd e Plaats om brandgevaar te voorkomen geen textiel papier of licht ontvlambare producten zoals benzine aceton thinner of alcohol in de buurt van de ingeschakelde lamp 6 Gevaarlijke preparaten Deze microscoop is voornamelijk bedoeld voor gebruik in microscopische onderzoek van levende celmateriaal en weefsel Controleer bij het verwerken van een preparaat altijd of het gevaarlijk is of niet Behandel gevaarlijke preparaten volgens de standaardprocedure voor uw laboratorium Draag als het preparaat infectiegevaarlijk is altijd rubber handschoenen om infectie te voorkomen en raak het p
39. 5 x 20 Italian 22 Safety Precautions r ZN avvertenza 5 Calore proveniente dalla sorgente luminosa Durante l uso la lampada si riscalda Quando la lampada accesa non rimuovere la lente di campo e prima di toccare la lampada verificare che sia stata spenta almeno per 30 minuti e Quando si sostituisce la lampadina della lampada verificare che la lampada si sia raffreddata abbastanza da poter essere toccata la luce deve essere spenta almeno per 30 minuti e Non toccare la lampada mentre accesa o prima che sia stata spenta almeno per 30 minuti in caso contrario si pu incorrere in ustioni e Non avvicinare mai alla lampada calda vestiti carta o sostanze volatili infiammabili come benzina petrolio acetone diluente o alcool perch si potrebbe provocare incendi 6 Campioni pericolosi Questo microscopio riservato soprattutto all uso nell osservazione microscopica di cellule e tessuti fissati su vetrino Quando si maneggia un campione controllarlo per sapere se pericoloso Maneggiare i campioni pericolosi in conformit alla procedura standard del proprio laboratorio Se il campione di natura infettiva indossare guanti di gomma per evitare l infezione e fare attenzione a non toccare il campione stesso Nel caso di contatto con un campione posto sul microscopio decontaminare la porzione contaminata in conformit alla procedura standard del proprio laboratorio r artENZIONE 1 Spegner
40. Auxiliary lens 8 Blue filter rotating the condenser focus knob 6 Place the blue filter in the frame provided and NA insert them into the bottom of the condenser e 7 Focus the condenser so that light passing Frame through the condenser focus the image on the correct position of the specimen center of the optical path e Microscopes without the field diaphragm Rotate the condenser focus knob to raise the condenser to its full limit and then slightly lower it to the position where the diffuser image can no longer be seen in the viewfield e Microscope with the field diaphragm 7 1 Focus on the specimen with the 10x objective 7 2 Close the field diaphragm to its minimum aperture by rotating the field diaphragm ring 7 3 Focus the field diaphragm image on the specimen surface by rotating the condenser focus knob 7 4 Center the field diaphragm image in the viewfield of the eyepiece by manipulating the centering screws 7 5 Change to the 40x objective and focus on the specimen by rotating the fine focus knob 7 6 Focus the field diaphragm image on the specimen surface by rotating the condenser focus knob 70 Chapter 6 Assembly 2 Assembly of Additional Components 7 7 Center the field diaphragm image in the viewfield of the eyepiece by manipulating the centering screws Centering is easier to perform if the size of the field diaphragm image is adjusted so that it is slightly smaller Field gt F
41. C strgmindtaget e Hvis indgangssp ndingen der er vist er forskellig fra det lokale str mniveau m mikroskopet ikke t ndes G r i stedet f lgende Sp ndingen p navnepladen afviger Kontakt din n rmeste Nikon repr sentant Sp ndingen over AC str mindtaget afviger ndr indstillingen for sp ndingen se side 68 29 Danish Safety Precautions z aovarse 1 Hensigtsmeessig brug af udstyret Dette mikroskop er fremstillet hovedsagligt til brug for mikroskopisk observation af celler og stof fastgjort p sl den ved hj lp at diaskopi transmitteret og episkopi reflekteret belysning Som hovedform l er det designet til eksperimenter og observation p hospitaler og andre laboratorier af s danne celler og stof inden for patologiske og cytologiske omr der 2 M ikke adskilles Adskillelse kan medf re d rlig funktion og eller elektrisk st d og indeb rer oph velse af alle garantier Adskil ikke andre dele end dem der er beskrevet i denne vejledning Hvis du oplever problemer med mikroskopet kontaktes n rmeste Nikon repr sentant 3 Kontrol af indgangssp ndingen Su Indgangsspaendingen er angivet p to steder bag p mikroskopet p navnepladen og over AC strgmindgangen Kontroller at disse angivelser af indgangssp ndingen svarer til den sp nding der leveres i dit omr de Hvis ikke f lges instruktionerne nedenfor Brug af mikroskopet med forskellig angivelse af indgangss
42. Contact with skin Rinse your skin thoroughly with soap and water Contact with eye Rinse your eye thoroughly with water more than 15 minutes and see a doctor e Do not leave immersion oil in the sun ultraviolet rays can damage it 63 Chapter 5 Miscellaneous Operations 2 Adjusting the Torque of the Coarse Focus Knob BE Adjusting the Torque of the Coarse Focus Knob The tension torque of the coarse focus knob rotation can be adjusted To increase the tension turn the coarse focus knob torque adjustment ring counterclockwise The torque adjustment ring is located at the back of the coarse focus knob To decrease the tension turn the ring clockwise Do not decrease the tension too much If it is too Tightens loose the stage will fall under its own weight Loosens ES Upper Limit Bolt The upper limit bolt is used to prevent the specimen touching the objective when a 40x or larger power objective objectives with a small working distance is used Using the upper limit bolt the stage does not move up from a certain position Attach the bolt as follows 1 Bring an objective with 40x or larger power into the optical path Focus on the specimen Move the bolt from the left to the right hole 2 Lower the stage to slightly below the position set in step 1 3 Remove the field lens unit 4 A hexagonal socket head bolt is located in the left hole at the lower part of the focusing mechanism
43. M313E 04 2 cr2 Nikon ECLIPSE E200 Instructions Thank you for purchasing the Nikon products This instruction manual is written for the users of the Nikon s microscope ECLIPSE E200 and describes the basic operations of the microscope To ensure correct usage read this manual carefully before operating the instrument e It is prohibited to reproduce or transmit this manual in part or whole without Nikon s expressed permission e The contents of this manual are subject to change without notice e Although every effort has been made to ensure the accuracy of this manual if you note any points that are unclear or incorrect contact your nearest Nikon representative e Some of the products described in this manual may not be included in the set you have purchased e Be sure to read the instruction manual for any other products used in combination with the microscope Contents 2 Contents Safety Precautions ossis sisiane ana Sana a diene ONNEEN NN ad aN 4 la EEE 4 Germani ds eta dada ETET 8 TE SEEN ve a 12 Spanish A ne 16 Italian 20 D tch elie A VS ee 24 DELE 28 near ON 32 A E des ete PE PEPE PEPE Rte 36 AE 40 Notes on Handling the System ii 44 Chapter 1 Nom
44. Remove the bolt using a supplied hexagonal wrench and screw it into the right hole Note The upper limit bolt may not work well depending on the thickness of the specimen or the microscope itself The specimen may touch the 40x objective or the stage may be stopped by the limit before the specimen image is in focus Check that the bolt works before relying on it 64 Chapter 5 Miscellaneous Operations 4 Eye Level Raiser EEE Eye Level Raiser The eye level raiser optional adjusts the height of the eyepiece tube according to the eye level of the user Install the eye level raiser between the basic unit and the eyepiece tube One eye level raiser is 25 mm high You can use up to two raisers this raises the eyepiece tube by 50 mm Use a hexagonal wrench nominal size 2 for M4 set screws to install the raisers a Photomicrography and TV Microscopy The microscope ECLIPSE E200 can be used for photomicrography and TV microscopy For those observations trinocular eyepiece tube photomicrographic equipment TV vertical tube and C mount CCTV camera optional are available Read the manuals provided with these devices for installation and operating conditions Eyepiece tubes and relay lenses come in various types For details ask your nearest Nikon representative Trinocular Eyepiece Tube Push or pull the optical path selection lever to adjust the amount of light sent to the binocular part and the vertical t
45. a dry cloth Short circuiting can also result when foreign matter is trapped inside the microscope If foreign matter or water has entered the microscope do not use the microscope and contact your nearest Nikon representative 3 Stage Rack The rack for the stage protrudes during use Be careful not to hit the rack with your hand or other parts of your body when handling the microscope as you may get hurt by an edge of the rack 4 Disposal of the Microscope To avoid biohazard risk dispose of the microscope as the contaminated equipment according to the standard procedure for your laboratory 7 English Safety Precautions SicherheitsmaBnahmen In dieser Anleitung verwendete Warn MIES lO is Obwohl die Nikon Produkte dazu entworfen wurden Ihnen eine maximale Sicherheit beim Gebrauch zu bieten kann falscher Gebrauch oder Missachtung der Anleitungen zu Personen oder Sachsch den f hren und resultiert im Verlust aller Garantieanspr che Lesen Sie diese Anleitungen im Sinne Ihrer eigenen Sicherheit gr ndlich durch bevor Sie das Instrument einsetzen Diese Anleitung nicht wegwerfen sondern aus Sicherheitsgr nden stets in der N he des Produktes aufbewahren um bei Bedarf schnell darin nachschlagen zu k nnen In dieser Anleitung werden Sicherheitshinweise mit den folgenden Symbolen gekennzeichnet Achten Sie darauf die mit diesen Symbolen gekennzeichneten Anleitungen zu ber cksichtigen um einen ordnungsgem en und s
46. a ja heti k yt n j lkeen e Palovammojen vaara l kosketa lamppua tai ymp r ivi alueita k yt n aikana tai heti k yt n j lkeen e Varmista ett lamppu ja sit ymp r iv alue on j htynyt riitt v sti ennen kuin yrit t lampun vaihtoa Varotoimet T m symbolietiketti kiinnitettyn l helle vaihtovirtapistoketta muistuttaa sinua seuraavasta e Tarkista tuloj nnite ennen kuin laitat mikroskoopin p lle Tuloj nnite annetaan nimikilvess ja vaihtovirtapistokkeen yl puolella e Jos n ytetty tuloj nnite eroaa paikallisesta j nnitetasosta l laita mikroskooppia p lle Tee sen sijaan seuraavat toimenpiteet Eri j nnite nimikilvess Ota yhteys l himp n Nikonin edustajaan Eri j nnite vaihtovirtapistokkeen yl puolella Vaihda tuloj nnitteen asetusta katso sivu 68 37 Finnish Safety Precautions E AN varorrus 1 Laitteen k ytt tarkoitus T m mikroskooppi on tarkoitettu p asiassa mikroskoopilla teht vien solujen ja kudoksen havaintojen tekoon n ytelevyilt k ytt en diaskooppista valoa l hetetty valoa ja pintavalaisua heijastettua valoa Se on suunniteltu p asiassa k ytett v ksi koetoimintaan ja havainnointiin sairaaloissa tai muissa laboratorioissa sellaisten solujen ja kudosten tutkimiseen joita patologia ja sytologia tutkivat 2 Ei saa purkaa Purkaminen saattaa aiheuttaa toimintah iri n ja tai s hk iskun ja johtaa kaikkien ta
47. acordo com o procedimento padr o do laborat rio A Cuidado com as altas temperaturas Este s mbolo aposto perto da unidade de lente de campo a l mpada est instalada sob a unidade de lente de campo chama a aten o do seguinte e A l mpada e as suas reas circundantes incluindo a unidade de lente de campo ficam muito quentes durante e imediatamente depois da ilumina o e Risco de queimaduras N o toque na l mpada ou reas circundantes durante e imediatamente depois da ilumina o Certifique se de que a l mpada e as suas reas circundantes arrefeceram suficientemente antes de tentar substituir a l mpada Precau es Esta etiqueta de s mbolo aposta perto da entrada de corrente alterna chama a aten o para o seguinte Antes de ligar o microsc pio verifique a tens o de entrada A tens o de entrada encontra se na placa de tipo e acima da entrada de AC e Se a tens o de entrada for diferente do n vel de tens o local n o ligue o microsc pio Em vez disso fa a o seguinte Tens o diferente na placa de tipo Contacte o representante da Nikon mais pr ximo Tens o diferente em cima da entrada de AC Altere a defini o da tens o de entrada consulte a p g 68 33 Portuguese Safety Precautions ES Naviso 1 Utiliza o projectada do equipamento O presente microsc pio destina se principalmente observa o microsc pica da c lulas e tecido apostos no
48. ag low breaking type fuses are used Power Cord Use the power cord provided 46 Chapter 1 Nomenclature of Each Part 2 Eyepieces 3 Eyepiece Tube 4 Objectives 7 Lamp 47 Switches and Controls 1 Diopter Ring Adjust the diopter ring to compensate for the difference between your right and left eyesight p 57 2 Revolving nosepiece Can hold up to four objectives 3 Stage 4 Specimen holder Put your finger at the root or the tip tilt of the claw to open the claw p 55 71 5 Condenser aperture diaphragm lever Set the lever to match the magnifying power of the objective p 59 6 Condenser Clamp Screw 7 Auxiliary lens for the condenser Screw on to the bottom of the condenser 8 Blue filter and the filter holder 9 Longitudinal stage motion Y Axis knob 10 Lateral stage motion X Axis knob These knobs are located either to the right or the left of the stage 11 Field diaphragm ring Set the ring to match the magnifying power of the objective p 60 This ring is equipped only on the microscope with a field diaphragm 12 Field diaphragm centering screws Used to center the field diaphragm image p 57 These screws are equipped only on the microscope with a field diaphragm 13 Fine focus knob Used for focusing There is no coarse focus knob on the side with the stage motion knobs 48 14 Power switch When turned to power is
49. ar la llave de energ a Indicaciones de la tensi n de entrada Para el microscopio con la plaqueta de caracter sticas que indique 100 110 120V La tensi n puede ajustarse a 100V 110V o 120V CA Para el microscopio con la plaqueta de caracter sticas que indique 220 230 240V La tensi n puede ajustarse a 220V 230V o 240V CA 4 Use la l mpara fusible y cable de energ a especificados Use la l mpara y fusible especificados Use el cable de energ a especificado El uso de la l mpara fusible o cable de energ a incorrectos puede da ar el instrumento o causar el incendio Vea tambi n la p gina 85 sobre el cable de energ a En el caso de utilizar el cable de extensi n utilice solamente el cable que incluya el conductor a tierra de protecci n PE e L mpara especificada L mpara hal gena de 6V 20W PHILIPS 7388 u OSRAM HLX64250 o L mpara hal gena de 6V 30W PHILIPS 5761 Fusible especificado 250V 1A tipo de fusi n retardada con baja capacidad de interrupci n fusible en miniatura 5x20 x2 Spanish 18 Safety Precautions ZN Roverrencra 5 Calor de la fuente de iluminaci n La l mpara adquiere calor durante el uso No desmonte la unidad de la lente de campo cuando la l mpara est encendida y asegure que la l mpara haya quedado apagada durante 30 minutos antes de tocarla e Al cambiar la l mpara aseg rese de que la l mpara est lo suficientemente fr a para tocar
50. autre dysfonctionnement Si le microscope est clabouss d eau mettez imm diatement hors tension basculez l interrupteur sur O et d branchez le cordon secteur Puis liminez l eau avec un chiffon sec Un court circuit peut aussi survenir de mati res trang res pi g es dans le microscope Si des mati res trang res ou de l eau ont p n tr dans le microscope ne I utilisez pas et contactez l agent Nikon le plus proche 3 Pignon de la platine Le pignon de platine saillit pendant l emploi Prenez garde de ne pas heurter le pignon de la main ou avec une autre partie du corps pendant la manipulation du microscope car vous pourriez vous blesser sur son extr mit 4 Mise au rebut du microscope Pour viter tout risque biologique jetez le microscope en tant qu appareil contamin conform ment la proc dure standard de votre laboratoire 15 French Safety Precautions Precauciones de Seguridad Simbolos de Advertencia Precauci n de este manual Pese a que los productos Nikon est n dise ados para garantizar la m xima seguridad durante su uso el uso incorrecto o al no respetarse las precauciones puede causar lesiones personales o da os materiales Las averias y los da os debidos a estos actos pueden quedar excluidos del derecho a todos los reclamos contra la garantia Para su seguridad lea cuidadosa y enteramente el manual de instrucciones antes de usar el instrumento Se ruega no descartar este
51. ca de tipo com a indica o 100 110 120 V poss vel definir a tens o para AC 100 V 110 V ou 120 V Se o microsc pio apresentar uma placa de tipo com a indica o 220 230 240 V poss vel definir a tens o para AC 220 V 230 V ou 240 V Indicac es de tens o de entrada 4 Use a l mpada o fusivel e o cabo el ctrico especificados Utilize a l mpada e o fus vel especificados Utilize o cabo el ctrico fornecido Se utilizar uma l mpada fusivel ou cabo el ctrico incorrecto isso pode danificar o instrumento ou provocar um inc ndio Consulte igualmente a p g 85 sobre o cabo el ctrico Se utilizar uma extens o el ctrica utilize somente um cabo com um fio de terra de protecc o e L mpada especificada L mpada de halog neo de 6V 20W PHILIPS 7388 ou OSRAM HLX64250 ou l mpada de halog neo 6V 30W PHILIPS 5761 e Fus vel especificado 2 fusiveis miniatura de 250 V 1A de temporizac o e de quebra reduzida 5x20 Portuguese 34 Safety Precautions Naviso 5 Aquecimento da fonte el ctrica Durante o funcionamento a l mpada fica quente N o retire a unidade da lente de campo se a l mpada estiver ligada e espere no m nimo 30 minutos at poder tocar nela e Ao substituir a l mpada certifique se de que a l mpada arrefeceu o suficiente para poder tocar nela a luz deve estar desligada h pelo menos 30 minutos e N o toque na l mpada se estiver ligada
52. ccording to the selected objective If the microscope is equipped with a field diaphragm also adjust the size field diaphragm see the procedures 9 and 10 Aperture Diaphragm Adjustment Set the condenser aperture diaphragm lever to the same figure as the magnification of the objective in the optical path Corresponding objective magnification 100X 0X 20X 10x 4x Aperture diaphragm lever 58 Chapter 4 Microscopy Detailed Procedure Adjusting the Aperture Diaphragm The aperture size is increased or decreased by rotating the condenser aperture diaphragm lever If the aperture diaphragm is closed the brightness and resolution are decreased but the contrast and range of focus are increased If the aperture diaphragm is opened the brightness and resolution are increased but the contrast and range of focus are decreased Generally a good image of sufficient contrast can be achieved with the aperture diaphragm closed to approximately 70 80 of the objective s numerical aperture Since the image resolution will be degraded when the aperture diaphragm is closed too much do not close the aperture diaphragm to less than 60 of the objective s numerical aperture except when observing a specimen with low contrast such as a near transparent specimen The aperture diaphragm controls the numerical aperture of the illumination Do not use it to control brightness Use the brightness control dial to control the brightness e
53. ctrical shock or damage to the instrument turn off the power switch turn to O and unplug the power cord before replacing the fuse e Use the specified fuse Using a different fuse may damage the instrument or cause a fire hazard Specified Fuse 250V 1A time lag low breaking type 5x20 miniature fuse x2 ZN caution e Make sure that the contact of the fuse is not damaged before installing a new fuse If the contact is damaged a malfunction or overheating may result e Attach the fuse to the fuse holder securely If not the fuse may come loose or a contact failure may occur resulting in overheating or smoke e Put back the fuse holder securely to its original position 1 Turn off the power switch turn to O and unplug the power cord 2 Remove the fuse holder using a flatblade screwdriver 3 Remove the fuses and attach new fuses 4 Make sure that the voltage indication shown in the window of the fuse holder corresponds to the voltage provided in your region If not change the voltage setting by relocating the voltage selector For details see P 68 5 Put the fuse holder back in place Fuse Holder Voltage Indication 74 Optical Characteristics Combinations of 10x Field No 20 Eyepiece with E Plan Objectives Objective Total Numerical Real Depth of Resolving Working Magnification Magnification Aperture Viewfield Focus Power Distance 4x 40x 0
54. d with a field diaphragm correct positioning so that the field diaphragm image is Condenser position too low p focused on the specimen surface P 57 If the microscope is not equipped with a field diaphragm position the condenser slightly lower than the upper limit Using the voltage selector select the Incorrect input voltage voltage that corresponds to the voltage provided in your region P 68 Also see the causes and corrective measures for electrical problems 80 Chapter 8 Troubleshooting Tables 2 Mechanical Problems EE Mechanical Problems Image cannot be focused with high power objectives Causes Corrective Measures z Turn over the slide so that the cover Slide upside down glass faces up Use a cover glass of the specified Cover glass too thick gt ee 17 mmi High power objective contacts slide when changed over from low power Causes Corrective Measures Turn over the slide so that the cover Slide upside down gt glass faces up Use a cover glass of the specified Cover glass too thick gt 0 17 mm Diopter not adjusted correctly Adjust P 57 Difference in focal point too large when switching from one objective to another Causes Corrective Measures Diopter not adjusted correctly Adjust P 57 81 Chapter 8 Troubleshooting Tables 2 Mechanical Problems Specimen image jumps when specimen is moved by the stage Causes Corrective Meas
55. den Strom ausschalten den Netzschalter in die O Stellung bringen und den Stecker aus der Steckdose ziehen AnschlieBend das Wasser mit einem St ck trockenen Tuch abwischen Kurzschl sse k nnen auch durch in das Mikroskop eingedrungene Fremdk rper hervorgerufen werden Wenn Wasser oder Fremdk rper in das Mikroskop eingedrungen sind benutzen sie dieses nicht mehr und setzen sich mit Ihrer n chsten Nikon Vertretung in Verbindung 3 Zahnstange fiir den Objekttisch Beim Gebrauch tritt die Zahnstange fur den Objekttisch Uber diesen hinaus Achten Sie darauf beim Umgang mit dem Zahnstange Mikroskop nicht mit der Hand oder anderen K rperteilen dagegenzustoBen da Sie sich sonst an den Kanten der Zahnstange verletzen k nnten 4 Entsorgen des Mikroskops Entsorgen Sie das Mikroskop als kontaminiertes Ger t entsprechend der Standardprozeduren in Ihrem Labor um biologische Gefahren zu vermeiden 11 German Safety Precautions R gles de s curit Symboles Avertissement Attention utilis s dans ce manuel A Bien que les produits Nikon soient con us pour une s curit extr me pendant l emploi un emploi incorrect ou la n gligence des instructions peut provoquer des blessures ou d g ts mat riels ce qui se traduira par la perte par d ch ance de tout droit sous garantie Pour votre s curit lisez attentivement et enti rement ce mode d emploi avant l utilisation de l appareil Ne le jetez pas conserve
56. du papier ou des mat riaux volatils inflammables tels que l essence benzine ac tone diluant ou alcool de la lampe chaude sinon un incendie est possible 6 Echantillons dangereux Ce microscope est essentiellement destin l observation microscopique de cellules et tissus fix s sur des lames porte objet A la manipulation d un chantillon d terminez s il est dangereux Manipulez les chantillons dangereux conform ment la proc dure standard de votre laboratoire Si l chantillon est de type infectieux portez des gants en caoutchouc pour viter l infection et prenez garde de ne pas le toucher En cas de contact de l chantillon avec le microscope d contaminez la partie contamin e conform ment la proc dure standard de votre laboratoire AN ATTENTION 1 Mettez hors tension avant l assemblage du microscope le remplacement de l ampoule ou du fusible et le branchement d branchement du cordon secteur Mettez hors tension avant de brancher ou d brancher le cordon secteur pour viter toute d charge lectrique ou incendie Mettez aussi hors tension puis d branchez le cordon secteur avant d assembler le microscope et avant de remplacer l ampoule ou le fusible Pour mettre hors tension basculez l interrupteur sur O 2 Maintenez le microscope exempt d humidit et de mati res trang res Maintenez le microscope exempt d humidit pour viter tout court circuit pouvant r sulter en surchauffe ou un
57. e e If the voltage indication above the AC inlet differs gt Refer to p 68 and change the input voltage setting before turning on the power switch For the microscope with the nameplate showing 100 110 120 V The voltage can be set to AC 100 V 110 V or 120 V For the microscope with the nameplate showing 220 230 240 V The voltage can be set to AC 220 V 230 V or 240 V Input Voltage Indications 4 Use the Specified Lamp Fuse and Power Cord Use the specified lamp and fuse Use the power cord provided Using an incorrect lamp fuse or power cord may damage the instrument or cause a fire Also see p 85 on power cord If using an extension cord only use a cord that includes a protective earth PE wire e Specified Lamp Halogen lamp 6V 20W PHILIPS 7388 or OSRAM HLX64250 or Halogen lamp 6V 30W PHILIPS 5761 e Specified Fuse 250 V 1A time lag low breaking type 5x20 miniature fuse x2 English 6 Safety Precautions ZN warnine 5 Heat at the Light Source The lamp becomes hot during use Do not remove the field lens unit while the lamp is on and be sure the lamp has been off for 30 minutes before touching it e When changing the lamp bulb make sure that the lamp is cool enough to touch the light should be off at least 30 minutes e Do not touch the lamp while it is on or until the lamp has been off for 30 minutes as doing so could result in burns e Never bring cloth paper
58. e Turn and align the diopter ring on the left eyepiece in the same way Specimen Mounting Place specimen slide on the stage with the coverglass facing upward Open the claw of the specimen holder with your finger at the root or the tip tilt and fix the specimen slide with the claw Focus with the 10x Objective Rotate the revolving nosepiece to bring the 10x objective into the optical path The objective will click into place when rotated into position Bring the specimen image into focus by turning the coarse focus and then fine focus knob e Direction of stage movement relative to focus knob rotation is shown in the figure e There is no coarse focus knob on the side with the stage motion knobs There are both coarse and fine focus knobs on the opposite side of the stage motion knobs e Do not turn the right and left focus knobs simultaneously in the opposite directions Do not turn the coarse focus knob further after the stage has reached its lower or upper limit These operations could result in a malfunction 55 Match the bottom edge of the diopter ring with the engraved base line Chapter 4 Microscopy Detailed Procedure Focusing Turning the focus knobs recklessly is a long and hard way to focus on the image If you are using a high power objective you may even damage the specimen by pressing it against the objective Before breaking the coverglasses or damaging the objectives read the followi
59. e careful not to hit the objective with the specimen 1 1 Turning Off the Lamp Turning off the power switch turn to O switches off the lamp When storing the microscope e Unplug the power cord e Wait until the field lens unit is cool enough to touch e Return the binocular part to its lowest position e Cover the microscope with the vinyl dust cover Before covering the microscope make sure that the field lens unit is cool enough to touch e When carrying the microscope hold it at its upper rear and lower front ends 61 Miscellaneous Operations Oil Immersion Observation The Oil mark on the side of an objective indicates that it is an oil immersion type objective The oil immersion objective also has a black band around the barrel end An oil immersion objective is used with the immersion oil applied between the front of the objective and the coverglass For an oil immersion objective with a numerical aperture of 1 0 or more use of an oil immersion type condenser is required to take full advantage of its performance An oil immersion type condenser like an oil immersion type objective needs immersion oil to be applied between the front of the condenser and the coverglass The abbe condenser included in the bright viewfield set can be used for oil immersion observation The condenser has an oil receptacle around its front lens Example of Oil Immersion Condenser Move the specimen toward the
60. e l interruttore dell alimentazione prima di assemblare il microscopio sostituire la lampada o il fusibile e inserire il cavo di alimentazione nella presa di rete o disinserirlo da essa Prima di inserire il cavo di alimentazione nella presa di rete o di disinserirlo da essa spegnere l interruttore di alimentazione per prevenire folgorazioni o incendi Inoltre spegnere l interruttore dell alimentazione e poi disinserire il cavo di alimentazione prima di assemblare il microscopio e prima di sostituire la lampada o il fusibile Per spegnere l alimentazione impostare l interruttore dell alimentazione su O 2 Mantenere il microscopio libero da umidit e da oggetti estranei Mantenere il microscopio libero da umidit per prevenire cortocircuiti che potrebbero provocare surriscaldamento o altri malfunzionamenti Se si versa dell acqua sul microscopio spegnere immediatamente l interruttore dell alimentazione impostare l interruttore su O e disinserire il cavo di alimentazione dalla presa di rete Quindi togliere l acqua con un panno asciutto Si pu verificare un cortocircuito anche quando materiale estraneo rimane intrappolato all interno del microscopio Se nel microscopio penetrato materiale estraneo oppure entrata dell acqua non utilizzare l apparecchio e contattare subito il rappresentante Nikon pi vicino 3 Intelaiatura del piatto portaoggetti Durante l uso l intelaiatura del piatto portaoggetti sporge Quando
61. e will also be off centered in the viewfield especially when closed down Follow the steps below to match the centers of the viewfield and the field diaphragm image Close down the field diaphragm 1 Swing the 10x or 4x objective in the optical path Close the field diaphragm to its minimum aperture by rotating the field diaphragm ring Continued on the following page 57 Chapter 4 Microscopy Detailed Procedure 2 While looking into the eyepieces rotate Field diaphragm image the condenser focus knob to focus the field diaphragm image on the specimen surface Focus on the edge of the diaphragm image Then center Focus on the the imad 3 Rotate the field diaphragm centering field ge screws until the field diaphragm image diaphragm comes to the center of the viewfield image with this knob 4 Switch to the 40x objective and rotate the field diaphragm ring so that the field diaphragm image becomes about the 40x Z same size as the viewfield Open the field Y 5 Ifthe field diaphragm image is not diaphragm till centered use the centering screws the field in diaphragm agam image circumscribes the viewfield Then center the image Focus on the field diaphragm image with this knob Objective Selection Rotate the revolving nosepiece to the desired objective magnification The objective will click into place when rotated into position Adjust the aperture diaphragm lever a
62. earest Nikon representative Different voltage above the AC inlet Change the input voltage setting refer to P 68 5 English Safety Precautions E WARNING 1 Intended use of the equipment This microscope is intended mainly for use in microscopic observation of cells and tissue fixed on the slide using diascopic transmitted and episcopic reflected illumination It is designed for the main purposes of experimentation and observation in hospitals or other laboratories of such cells and tissue within the fields of pathology cytology 2 Do not disassemble Disassembly may cause malfunction and or electrical shock and will lead to the forfeiture of all claims against warranty Do not disassemble any part other than those described in this manual If you experience any problem with the microscope notify your nearest Nikon representative 3 Check the Input Voltage The input voltage is indicated in two places at the rear of the microscope on the nameplate and above the AC inlet Confirm that these input voltage indications correspond to the voltage provided in your region If not follow one of the instructions below The use of microscopes with the different input voltage indications will cause overcurrent and overheating which may result in fire or severe damage to the microscope e If the voltage indication on the nameplate differs gt Do not plug in the microscope Contact your nearest Nikon representativ
63. eed for caution at all times during use Always refer to the instruction manual and read the relevant instructions before manipulating any part to which the symbol has been affixed Symbol Meaning Caution Biohazard A This symbol label attached on the stand reminds you of the following e WARNING Contact of a sample to the microscope presents a biohazard risk e To avoid biohazard contamination do not touch the contaminated portion with your bare hands e Decontaminate the contaminated portion according to the standard procedure for your laboratory Caution for heat IN This symbol label attached near the field lens unit the lamp is set underneath the field lens unit reminds you of the following e Lamp and its surrounding areas including the field lens unit become very hot during and immediately after illumination e Risk of burns Do not touch the lamp or surrounding areas during and immediately after illumination e Make sure the lamp and its surrounding areas has cooled sufficiently before attempting to replace the lamp Caution This symbol label attached near the AC inlet reminds you of the following e Check the input voltage before turning on the microscope The input voltage is given on the nameplate and above the AC inlet e If the input voltage shown differs from the local voltage level do not turn on the microscope Do the following instead Different voltage on the nameplate Contact your n
64. enclature of Each Part annen sisi 46 Chapter 2 Switches and Controls nonnen eene verven eenn ER REE REE ELLLER ERE ERE 48 Chapter 3 A Quick Microscopic Procedure 50 Chapter 4 Microscopy Detailed Procedure 54 Chapter 5 Miscellaneous Operations aka aan annan 62 1 Oil Immersion Observation isisi 62 2 Adjusting the Torque of the Coarse Focus Knob ss 64 3 Upper LIMmit Bolt aa LL ee 64 4 Eye tevel Raiser 65 5 Photomicrography and TV MicroSCOpy eneeneneneeneneneeneneneenenenennenenennenenenn 65 6 ND Filter for the Objective an na naan nn nn nun ENES ES ESSEN aan aan 66 7 Cord H nger 67 Chapter 6 Assembly 68 1 Assembly of Standard 56 en 68 2 Assembly of Additional Components n ii 70 T Condense a ad vann wedde 70 2 Oo Eea LS 71 3 SpecimeniMold eti Aiden fe de rega seda M sten do da RE ank eenen eenden wend denk me eend 71 4 Eyepiece and Eyepiece Pointer sise 71 5 Other Accessories nn a SSN ae a 71 3 Replacement of Consumable Materials sise 72 1 Replacing the dan vand a vand anak ven ene va eue 72 2
65. ennungsgefahr Die Lampe und deren Umgebung nicht w hrend oder unmittelbar nach der Beleuchtung ber hren e Achten Sie darauf dass sich die Lampe und deren Umgebung ausreichend abgek hlt hat bevor Sie versuchen die Lampe ersetzen Vorsicht Dieser in der N he des Netzanschlusses angebrachte Symbolaufkleber macht Sie auf folgendes aufmerksam e Pr fen Sie vor Einschalten des Mikroskops die Eingangsspannung Die Eingangsspannung wird auf dem Typenschild und ber dem Netzanschluss angegeben e Das Mikroskop nicht einschalten wenn sich die Eingangsspannung von der lokalen Netzspannung unterscheidet Machen Sie statt dessen folgendes Bei Angabe einer anderen Spannung auf dem Typenschild Setzen Sie sich mit Ihrer n chsten Nikon Vertretung in Verbindung Andere Spannung ber dem Netzanschluss ndern Sie die Einstellung f r die Eingangsspannung siehe Seite 68 9 German Safety Precautions N warnuns 1 Verwendungszweck Dieses Mikroskop ist haupts chlich fur mikroskopische Beobachtungen von Zellen und fixierten Gewebeproben mit Hilfe diaskopischer durchscheinender und episkopischer reflektierter Beleuchtung konzipiert Es ist haupts chlich fur Experimente und Beobachtungen derartiger Zellen oder Gewebeproben in Krankenh usern oder anderen Laboratorien in den Bereichen Pathologie und Zytologie gedacht 2 Nicht auseinanderbauen Auseinanderbauen kann zu St rungen und oder elektrischen Sch
66. ere i tilskadekomst eller materiel skade Danish 28 Safety Precautions Betydning af symboler brugt p udstyret Symbolet der vises p instrumentet kr ver altid forsigtighed under brug Se altid i brugsanvisningen og l s de relevante instruktioner f r der manipuleres med dele der har et symbol p sat Symbol Betydning Forsigtig Biologisk risiko A Denne symboletikette der sidder pa standeren betyder f lgende e ADVARSEL Kontakt mellem pr ven og mikroskopet kan udg re en biologisk risiko For at undg kontaminering af biologisk risiko skal man ikke ber re den forurenede del med de bare h nder e Dekontaminer den kontaminerede del i overensstemmelse med standardprocedure i dit laboratorium Advarsel om varme IN Symboletiketten der sidder neer ved feltlinseenheden p ren er placeret under feltlinseenheden skal minde dig p folgende e P ren og dens omgivelser inklusive feltlinseenheden bliver meget varme under og lige efter brug e Risiko for forbr nding Ber r ikke p ren eller omgivelserne under og lige efter at lamperne har v ret i brug e Kontroller at p ren og dens omgivelser er tilstr kkeligt afk let f r man fors ger at udskifte p ren N Forsigtig Denne symboletikette der sidder t t ved stramindtaget skal minde dig om f lgende e Kontroller indgangssp ndingen f r mikroskopet t ndes Indgangsspaendingen er opgivet p navnepladen og over A
67. gende e Controleer de ingangsspanning voordat u de microscoop inschakelt De ingangsspanning is vermeld op het typeplaatje en boven de wisselspanningsaansluiting e Schakel de microscoop niet in als de ingangsspanning afwijkt van de netspanning ter plaatse Ga in dat geval als volgt te werk De netspanning komt niet overeen met de ingangsspanning op het typeplaatje Neem contact op met de dichtstbijzijnde Nikon vertegenwoordiging De netspanning wijkt af van de ingangsspanning die vermeld is boven de wisselspanningsaansluiting Wijzig de instelling voor de ingangsspanning zie pag 68 25 Dutch Safety Precautions 4 ZN waarsch UWING 1 Bedoeld gebruik van de apparatuur Deze microscoop is voornamelijk bedoeld voor gebruik in microscopische onderzoek van celmateriaal en weefsel tussen glasplaatjes is voorzien van directe en gereflecteerde verlichting Hij is ontworpen voor toepassing bij experimenten en waarnemingen in ziekenhuizen en andere laboratoria van cellen en weefsel binnen het werkveld van de pathologie en de cytologie 2 Demonteer het apparaat niet Wanneer het apparaat gedemonteerd wordt kunnen storingen en of gevaar voor elektrische schokken ontstaan en zullen alle garantieclaims als gevolg daarvan afgewezen worden Demonteer geen andere componenten anders dan die beschreven zijn in deze handleiding Neem wanneer u onregelmatigheden aan de microscoop bemerkt contact op met de dichtstbijzijnde Nikon leve
68. he pointer to one of the eyepieces Rotate and remove the viewfield ring from the eyepiece end Attach the eyepiece pointer to the eyepiece and then put back the viewfield ring Other Accessories For installation of other accessories such as the photomicrographic equipment see the manual provided for each product 71 Chapter 6 Assembly 3 Replacement of Consumable Materials En Replacement of Consumable Materials 1 Replacing the Lamp ZN warnine e To avoid electrical shock or damage to the instrument turn off the power switch turn to O and unplug the power cord before lamp replacement e Use the specified lamp Using a different kind of lamp may damage the instrument or cause a fire hazard Specified Lamp Halogen lamp 6V 20W PHILIPS 7388 or OSRAM HLX 64250 or Halogen lamp 6V 30W PHILIPS 5761 E ZN caution e The lamp is hot when in use To avoid burn injury turn off the microscope and wait at least 30 minutes until the lamp is cool enough to touch before attempting to change the lamp e Make sure that the contacts of the lamp and socket are not damaged before installing a new lamp If the contacts are damaged they may cause poor illumination or overheating Insert the lamp s contact pins fully into the socket holes If the pins are loose the lamp could come loose or result in a contact failure which will cause overheating or smoke Be sure to put the field lens unit
69. he condenser and the parts under the condenser 60 Chapter 4 Microscopy Detailed Procedure Using the Working Distance for Focusing Each objective has its working distance indicated on its side The working distance is the distance between the front of the objective and the specimen when the specimen image is in focus If you have difficulties in focusing with the standard procedure described on p 56 try one of the following methods using the working distance for focusing Method 1 While looking at the microscope from the side rotate the coarse focus knob to bring the specimen close to the objective When the distance between the specimen and the front of the objective becomes slightly smaller than the working distance take your hands off the coarse focus knob The specimen is now almost in focus Look into the eyepieces and rotate the fine focus knob in the direction that lowers the stage Raise the stage until Lower the stage this distance is with the fine slightly smaller than focus knob to the working distance find the focal point Method 2 Swing the 40x objective into the optical path While looking at the microscope from the side rotate the coarse focus knob until the specimen almost touches the objective about 0 5 mm apart from the front of the objective The specimen is now almost in focus Switch to the 10x objective look into the eyepieces and rotate the fine focus knob slightly to find the focal point B
70. iaphragm Widen or narrow to merge the viewfields into on U Turn on the lamp gt O and adjust Viewfields interpupillary distance Here s the power switch Adjust brightness too Focus with 10x objective Then focus with 10X this ring Match the bottom 5 Use this knob for edge of the diopter a NS focusing ring with the N x engraved base line gt Use your left eye ol ADS Repeat to O twice lt a Er Use your JAN right eye And focus with this ring Adjust the diopter KA Focus with this s knob 52 Chapter 3 A Quick Microscopic Procedure Check the magnifying Turn off the power power Wait till the microscope cools down before storing Move the lever to the magnifying power of the objective Magnify the image and observe Field diaphragm image 48 Focus on the field diaphragm image with this knob Then diaphragm till the field Close down the diaphragm field diaphragm Field diaphragm image image circumscribes the viewfield O N Then center the Center the field Focus on gt image 5 the field diaphragm diaphragm Q El image with this knob 53 Microscopy Detailed Procedure 1 Lamp Illumination Turn on the power switch turn to and the lamp will come on Turn the brightness control dial to adjust the brightness of the viewfield Turning the dial clockwi
71. icheren Gebrauch zu gew hrleisten Symbol Bedeutung SN warnuns Missachtung von mit diesem Symbol SEKEIZEIEHNELEN Anleitungen kann zu t dlichen Unf llen oder schweren Verletzungen f hren AN vorsicht Missachtung von mit diesem Symbol gekennzeichneten Anleitungen kann zu Verletzungen oder Sachsch den f hren German 8 Safety Precautions Bedeutung der auf dem Ger t verwendeten Symbole Die auf den Ger t erscheinenden Symbole weisen darauf hin dass bei dessen Gebrauch stets Vorsicht geboten ist Beziehen Sie sich immer erst auf die entsprechenden Abschnitte in der Bedienungsanleitung bevor Sie mit diesen Symbolen gekennzeichnete Teile manipulieren Symbol Bedeutung Vorsicht Biogef hrdung A Dieses am Stativ angebrachte Symbol weist Sie auf folgendes hin e WARNUNG Das mit Proben in Beruhrung kommende Mikroskop stellen eine Biogefahrdung dar e Die kontaminierten Abschnitte nicht mit den bloBen H nden ber hren um eine biogef hrliche Kontaminierung zu vermeiden e Entseuchen Sie die kontaminierten Abschnitte entsprechend der Standardprozedur in Ihrem Labor Vorsicht Hei Der in der N he der Kollektivlinseneinheit angebrachte Aufkleber die Lampe ist unterhalb der Kollektivlinseneinheit angebracht macht Sie auf folgendes aufmerksam e Die Lampe und deren Umgebung einschlie lich der Kollektivlinseneinheit werden w hrend und unmittelbar nach der Beleuchtung sehr hei Verbr
72. ield iaph KES than the viewfield i en LQ E ne lt E ers Objectives Objectives are attached to the microscope before shipment When replacing an objective remove the specimen from the stage and lower the stage Remove the objective holding it with both hands Be careful not to drop the objective Screw a new objective into the revolving nosepiece Set the objectives in such an order that the objective magnification increases as the revolving nosepiece is turned clockwise as viewed from the top of the microscope Specimen Holder The specimen holder is attached to the stage before shipment When removing the holder loosen the two hexagonal socket head bolts furthermost from the specimen using the hexagonal wrench provided Eyepiece and Eyepiece Pointer Eyepiece The 10x eyepieces are attached to the eyepiece O Eyepiece pointer tube before shipment When removing an eyepiece loosen the eyepiece set screws using the hexagonal wrench provided and then remove the SD Viewfield ring eyepiece When attaching an eyepiece push the eyepiece down to its full limit and tighten up the set screw Be careful not to tighten too hard D When changing to a 15x eyepiece optional be Eyepiece sure to change both the right and left eyepieces together The right and left eyepieces should be of a the same magnification Ss The eyepiece pointer optional serves as a reference for pointing out the specimen Attach t
73. ijdens gebruik steekt de preparaatbevestingsplaat uit Voorkom dat u bij het gebruik van de microscoop de preparaatbevestingsplaat aanstoot met uw hand of een ander lichaamsdeel omdat u zich daarbij zou kunnen verwonden Bevestingsplaat 4 Afvoeren van de microscoop Voer de microscoop om biologische besmetting te voorkomen af als verontreinigde apparatuur volgens de standaardprocedure van uw laboratorium 27 Dutch Safety Precautions Sikkerhedsforanstaltninger Advarsels og forsigtighedssymboler anvendt i denne manual Selv om Nikon produkter er fremstillet s ledes at de yder dig den maksimale sikkerhed under anvendelsen kan forkert anvendelse eller tilsides ttelse af instruktionerne for rsage personskade eller materiel skade og vil medf re fortabelse af alle garantirettigheder For din sikkerheds skyld b r du l se instruktionsmanualen omhyggeligt og grundigt f r instrumentet anvendes Smid ikke denne manual v k men opbevar den i n rheden af produktet s den nemt kan konsulteres I denne manual er sikkerhedsinstruktionerne angivet med de herunder viste symboler S rg for at overholde instruktionerne der er angivet med disse symboler for at garantere korrekt og sikker operation Symbol Betydning AN ADVARSEL Dessen ee af instruktioner der er angivet med dette symbol kan resultere i d d eller alvorlig tilskadekomst A rorsicrie Tse teles af der er angivet med dette symbol kan result
74. k st d eller brand Afbryd ogs str mmen og tag derefter stikket ud f r samling af mikroskopet og f r udskiftning af p rer og sikringer Sluk for str mmen s t str mafbryderen p O 2 Hold mikroskopet fri for fugt og fremmedlegemer Hold mikroskopet fri for fugt for at forhindre kortslutning der kan resultere i overophedning eller andre funktions del ggelser Hvis der st nkes vand p mikroskopet slukkes straks for strommes s t afbryderen p O og stikket tages ud kontakten Derefter t rres vandet af med en t r klud Kortslutning kan ogs v re resultatet n r fremmedlegemer sidder fast inden i mikroskopet Hvis der er kommet fremmedlegemer eller vand ind i mikroskopet bruges mikroskopet ikke og n rmeste Nikon repr sentant kontaktes 3 Oph ng til objektbord Oph nget til objektbordet stikker frem under brug V r forsigtig ikke at ramme oph nget med h nden eller andre dele af kroppen n r mikroskopet betjenes da du kan blive sl et af oph ngets kant 4 Bortskaffelse af mikroskopet For at undg biologisk risiko bortskaffes mikroskopet ligesom det kontaminerede udstyr i overensstemmelse med standard procedure i dit laboratorium 31 Danish Safety Precautions Precauc es de seguranca S mbolos de aviso precau o utilizados neste manual na Apesar dos produtos Nikon serem concebidos para lhe fornecer a maior seguran a durante a sua utilizac o a utilizac o incorrecta ou o n o cum
75. ke feltlinseenheden n r p ren er t ndt og v r sikker p at p ren har v ret slukket i 30 minutter f r den ber res e N r der skiftes p re skal man s rge for at p ren er tilstr kkeligt afk let til at ber re lyset skal v ret slukket i mindst 30 minutter e R r ikke p ren mens der er t ndt eller f r p ren har v ret slukket i 30 minutter da det kan f re til forbr ndinger e S t aldrig p ren i n rheden af stof papir eller brandbare luftige v sker s som benzin petroleum benzin acetone fortynder eller alkohol da det kan resultere i brand 6 Farlig pr ve Dette mikroskop er fremstillet hovedsagligt til brug for mikroskopisk observation af celler og stof fastgjort p sl den N r en pr ve h ndteres kontrolleres det om pr ven er farlig H ndter farlige pr ver i overensstemmelse med standardprocedure i dit laboratorium Hvis pr ven er af smittefarlig natur b res gummihandsker for at undg infektion og der udvises forsigtighed for ikke at ber re en eneste af pr verne I tilf lde af kontakt mellem en pr ve og mikroskopet dekontamineres den forurenede del i overensstemmelse med standardprocedure i dit laboratorium A rorsicrie 1 Sluk p kontakten for mikroskopet samles Udskiftning af p re eller sikringer og Tilslutning og afbrydelse af str mforsyningsledning Sluk for str mafbryderen f r str mforsyningsledningen s ttes i stikket eller tages ud for at forhindre elektris
76. kuuoikeuksien menetykseen l pura mit n muuta osaa kuin niit jotka on kuvattu t ss ohjekirjassa Mik li mikroskoopin kanssa esiintyy ongelmia ota yhteys l himp n Nikonin edustajaan 3 Tarkista tuloj nnite Tuloj nnite osoitetaan kahdessa paikassa mikroskoopin takana Nimikilvess ja vaihtovirtapistokkeen yl puolella Varmista ett n m tuloj nnitemerkinn t vastaavat alueesi k ytt m j nnitett Mik li ei noudata yht alla olevista ohjeista Mikroskoopin k ytt eri tuloj nnitemerkinn ll voi aiheuttaa ylivirtaa ja ylikuumenemista mik voi johtaa tulipaloon ja mikroskoopin pahaan vaurioitumiseen e Jos j nnitemerkint nimikilvess on eri gt l liit mikroskooppia verkkovirtaan Ota yhteys l himp r Nikonin edustajaan Jos j nnitemerkint vaihtovirtapistokkeen yl puolella on eri gt Katso sivulta 68 ja vaihda tuloj nniteasetus ennen kuin k nn t virtakytkimen p lle Tuloj nnitelukemat Mikroskoopille jonka nimikilpi osoittaa 100 110 120 V J nnite voidaan asettaa AC 100 V 110 V tai 120 V Mikroskoopille jonka nimikilpi osoittaa 220 230 240 V J nnite voidaan asettaa AC 220 V 230 V tai 240 V 4 K yt m ritelty lamppua sulaketta ja verkkojohtoa K yt m ritelty lamppua ja sulaketta K yt mukana tullutta verkkojohtoa V r n lampun sulakkeen tai verkkojohdon k ytt voi vahingoittaa instrumenttia tai aiheuttaa tulipalon Katso my s
77. l gen f hren und resultiert dar ber hinaus im Verfall aller Garantieanspr che Keine anderen als die in der vorliegenden Anleitung beschriebenen Teile auseinanderbauen Wenn Sie Schwierigkeiten mit dem Mikroskop haben wenden Sie sich bitte an Ihren n chsten Nikon H ndler 3 Eingangsspannung pr fen Die Eingangsspannung ist an zwei Stellen an der R ckseite des Mikroskops angegeben dem Typenschild und ber dem Netzanschluss Achten Sie darauf dass die angegebene Eingangsspannung der Spannung der regionalen Stromversorgung entspricht Wenn dies nicht der Fall ist befolgen Sie bitte die nachstehenden Anleitungen Der Gebrauch des Mikroskops mit falscher Eingangsspannung kann zu berstr men und berhitzung f hren was wiederum zu Feuerentwicklung oder auch einer Besch digung des Mikroskops f hren k nnte e Bei Angabe einer anderen Spannung auf dem Typenschild gt Das Mikroskop nicht anschlie en Setzen Sie sich mit der n chsten Nikon Vertretung in Verbindung Andere Spannung ber dem Netzanschluss gt Beziehen Sie sich auf Seite 68 betreffs nderung der Einstellung der Eingangsspannung bevor Sie den Strom einschalten Bei Mikroskopen bei denen das Typenschild 100 110 120 V anzeigt Die Spannung kann auf 100 V 110 V oder 120 V Wechselstrom eingestellt werden Bei Mikroskopen bei denen das Typenschild 220 230 240 V anzeigt Die Spannung kann auf 220 V 230 V oder 240 V Wechselstrom eingestellt werden
78. ng and find the correct way to focus on the specimen 1 Put the 10x or 4x objective in the optical path 2 Turn the coarse focus knob to raise the stage to its upper limit 3 Looking into the eyepieces slowly rotate the coarse focus knob to lower the stage When the specimen image appears stop rotating the knob 4 Rotate the fine focus knob and precisely focus on the image When you want to observe the image with a high power objective first focus on the image using a 10x or 4x objective Then change to a high power objective and rotate the fine focus knob for precise focusing then look into the eyepieces and lower the First raise the stage o 1 1 When rotating the coarse focus knob while looking into the eyepieces be sure to turn it only in the direction that lowers the stage 2 When raising the stage using the coarse focus knob take your eyes off the eyepieces and look at the gap between the upper surface of the specimen and the front of the objective from the side 3 First focus with a low power objective Then change to a high power objective 1 Since the working distances of 10x and 4x objectives are large p 75 these objectives do not touch the specimen even when the stage is raised to its upper limit provided that the slide and coverglasses of a standard thickness are used The standard thickness for slides is 1 2 mm and that for coverglass is 0 17 mm 56 Chapter 4 Microscop
79. o the stage and the condenser which could lead to degraded performance N ZN warnine handling them When using petroleum benzine or absolute alcohol to wipe off immersion oil or to clean the lenses follow the instructions provided by their manufacturers Absolute alcohol and petroleum benzine are inflammable Take great care when 7 Shipping Clamps The microscope is held tightly by the clamps during shipme before use For details see p 69 45 Nomenclature of Each Part The microscope is made up of the following components 1 2 3 4 5 6 7 8 9 Basic unit Eyepieces Screwed on to the eyepiece tube Eyepiece Tube This is a binocular eyepiece tube A trinocular eyepiece tube is available for photomicrography and TV microscopy Objectives Objectives with various magnifying powers are available Condenser Used for condensing light The condenser should be positioned slightly lower than its upper limit Adjust the aperture diaphragm lever according to the objective Field Lens Unit Draw out the field lens unit when changing lamp The microscope may have a field diaphragm A field diaphragm is used to control the illumination range and should be adjusted according to the objective Note that there are two types of microscopes the one with the field diaphragm and the one without Lamp Halogen lamp 6 V 20 W or 6 V 30 W is used Fuse Two 250 V 1A time l
80. on 3 H KATA AMO TOU va TPAUNATIOTEITE and 4 H KATA X PI AMO KATA va TPAUJIATIOTEITE and 43 Greek Notes on Handling the System JSN Notes on Handling the System 1 Installation This microscope is a precision instrument Using the microscope in an unfavorable environment could result in malfunctions or degraded performance Consider the following conditions when choosing the installation location e Observation conditions are better if light from windows and bright room light can be avoided e Install the microscope in a location with a room temperature of 09 to 40 C and with a maximum relative humidity of 85 High temperature and humidity are to be avoided because they promote mold growth and condensation which may damage the microscope
81. one Attach the provided blue filter to the bottom of the condenser and check the color tone of the specimen Uneven Viewfield Brightness When a 4x objective is used for the photomicrographic equipment or digital still camera the viewfield may look partially dark Make the brightness of the viewfield uniform using one of the following methods e Lower the condenser Slightly lower the condenser to take more space between the specimen and the condenser If the microscope is equipped with a field diaphragm the field diaphragm image may blur slightly e Use a phase contrast condenser and diffuser slider both optional Attach the phase contrast condenser and the diffuser to the microscope When the diffuser is inserted into the optical path the amount of light will drop to about 60 of its initial value Also you will not be able to see the field diaphragm in the viewfield MON ND Filter for the Objective When observing the specimen with 10x and 40x objectives in turns placing an ND3 filter specially designed for objectives optional to the rear end of the 10x objective will facilitate the observation With the ND filter attached you will have the same brightness and color tones for both 10x and 40x objectives without adjusting the brightness control dial 66 Chapter 5 Miscellaneous Operations 7 Cord Hangers RES Cord Hangers Push the cord hangers optional into the holes on the rear of the microscope The hangers
82. or flammable volatile substances such as gasoline petroleum benzine acetone thinner or alcohol near a hot lamp as a fire could result 6 Hazardous Sample This microscope is mainly for use in microscopic observation of cells and tissue fixed on the slide When handling a sample check to determine whether the sample is hazardous Handle hazardous samples according to the standard procedure for your laboratory If the sample is of an infectious nature wear rubber gloves to avoid infection and be careful not to touch a sample In the event of contact of a sample to the microscope decontaminate the contaminated portion according to the standard procedure for your laboratory z ZN caution 1 Turn Off Power Switch before Assembling the Microscope Replacing the Lamp or Fuse and Plugging in or Unplugging the Power Cord Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or fire Also turn off the power switch and then unplug the power cord before assembling the microscope and before changing the lamp or fuse To turn off the power turn the power switch to O 2 Keep the Microscope Free of Moisture and Foreign Matter Keep the microscope free of moisture to prevent short circuiting that could result in overheating or other malfunctions If water splashes on the microscope immediately turn off the power switch turn the switch to O and unplug the power cord Then wipe off the water with
83. ou outras anomalias Se o microsc pio receber salpicos de gua desligue imediatamente o interruptor coloque o interruptor em O e desligue o cabo el ctrico Depois limpe a gua com um pano seco Os curto circuitos podem ocorrer se houver mat rias estranhas presas no interior do microsc pio Se mat rias estranhas ou gua ingressarem no microsc pio n o o utilize e contacte o representante da Nikon mais pr ximo 3 Cavalete SL LA Cavalete Durante a utiliza o o cavalete sobressai Tenha cuidado para n o atingir o cavalete com a m o ou outras partes do corpo ao manusear o microsc pio porque pode ferir se por uma das arestas do cavalete 4 Descarte do microsc pio Para evitar o risco biol gico descarte se do microsc pio como equipamento contaminado de acordo com o procedimento padr o do laborat rio 35 Portuguese Safety Precautions Turvallisuusohjeet T ss k sikirjassa k ytetyt varoitus varotoimet symbolit nn Vaikka Nikon tuotteet on suunniteltu mahdollisimman turvallisiksi k ytt voi v r k ytt tai n iden ohjeiden huomioimattomuus johtaa loukkaantumiseen tai omaisuusvahinkoihin ja siit seuraa kaikkien takuuseen liittyvien oikeuksien menetys Turvallisuuden vuoksi lue k ytt ohjeet huolellisesti ja perusteellisesti ennen laitteen k ytt l h vit t t k sikirjaa vaan pid se tuotteen l heisyydess voidaksesi helposti tarkistaa ohjeita Ohjek sikirjassa t
84. p nding vil for rsage overstr m og overophedning hvilket kan resultere i ild eller alvorlig skade p mikroskopet e Hvis sp ndingsangivelsen p navnepladen afviger gt Tilslut ikke mikroskopet Kontakt din n rmeste Nikon repr sentant e Hvis sp ndingsangivelsen over el indgangen afviger gt G til side 68 og ndr indstillingen for indgangssp ndingen f r der t ndes for str mmen For mikroskopet hvis navneplade viser 100 110 120 V Sp ndingen kan indstilles til AC 100 V 110 V or 120 V For mikroskopet hvis navneplade viser 220 230 240 V Sp ndingen kan indstilles til AC 220 V 230 V or 240 V Angivelser for ICI indgangssp nding 4 Benyt de angivne p rer sikringer og stromforsyningsledninger Benyt de angivne p rer og sikringer Brug den medf lgende stramforsyningsledning Brug af forkerte p rer sikringer eller str mforsyningsledninger kan beskadige instrumentet eller resultere i brand Se ogs s 85 om str mforsyningsledninger Hvis der bruges forl ngerledning anvendes kun en ledning der har en beskyttende jordforbindelse PE Specificerede p rer Halogenp re 6V 20W PHILIPS 7388 eller OSRAM HLX64250 eller Halogenp re 6V 30W PHILIPS 5761 e Specificerede sikringer 250 V 1A forsinkelses lavt afbrydende type 5x20 miniature sikring x2 Danish 30 Safety Precautions r aovarse 5 Varme ved lyskilden Paeren bliver varm under brug Fjern ik
85. ppia sill voit loukata itsesi telineen kulmaan Teline 4 Mikroskoopin h vitt minen V ltt ksesi biologisen vaaran h vit mikroskooppi kuten kontaminoitunut laite laboratorion normaalien menettelytapojen mukaisesti 39 Finnish Safety Precautions M popuhatgic OTO NAPOV EVXEIPIONG RS oT Ta Nikon ano ano
86. primento das instruc es pode causar les es pessoais ou danos no equipamento invalidando quaisquer reclamag es da garantia Para sua seguranca leia atentamente o manual de instruc es antes de utilizar o instrumento N o deite fora este manual guarde o perto do produto para f cil consulta Neste manual as instruc es de seguranca est o indicadas pelos s mbolos abaixo indicados Siga as instruc es indicadas nestes s mbolos para assegurar um funcionamento correcto e seguro S mbolo Significado A AVISO O n o cumprimento das instru es assinaladas com este s mbolo pode causar morte ou les es graves ZN precau o O n o cumprimento das instru es assinaladas com este s mbolo pode causar les es ou danos no equipamento Portuguese 32 Safety Precautions Significado dos simbolos utilizados no equipamento O simbolo que aparece no produto indica a necessidade de ter sempre cuidado durante a utilizag o Consulte sempre o manual de instruc es e leia as instruc es pertinentes antes de usar qualquer parte a que o s mbolo foi aposto S mbolo Significado A Precaug o Risco biol gico Esta etiqueta de s mbolo aposta na base chama a atenc o para o seguinte e AVISO O contacto entre a amostra e o microsc pio pode constituir um risco biol gico e Para evitar a contaminac o do risco biol gico n o toque na parte contaminada com as m os nus Descontamine a parte contaminada de
87. r El r tulo con este s mbolo adherido cerca de la unidad de la lente de campo la l mpara est debajo de la unidad de la lente de campo le recuerda lo siguiente e La l mpara y sus alrededores incluyendo la unidad de la lente de campo se tornan muy calientes durante e inmediatamente despu s de la iluminaci n e Existe el riesgo de quemaduras No toque la l mpara ni sus alrededores durante e inmediatamente despu s de la iluminaci n e Aseg rese que la l mpara y sus alrededores se hayan enfriado lo suficiente antes de intentar el reemplazo de la l mpara Precauci n El r tulo con este s mbolo adherido cerca de la entrada de CA le recuerda lo siguiente Verifique la tensi n de entrada antes de conectar el microscopio La tensi n de entrada est indicada en la plaqueta de caracter sticas y sobre la entrada de CA e Si la tensi n de entrada indicada difiere del nivel de la tensi n local no conecte el microscopio En ese caso proceda como sigue Tensi n que difiere de la plaqueta de caracter sticas Tome contacto con el representante Nikon m s pr ximo Tensi n que difiere de la indicaci n de la entrada de CA Cambie el ajuste de la tensi n de entrada Vea la P g 68 17 Spanish Safety Precautions soverrencra 1 Objeto del uso del equipo Este microscopio tiene como uso principal la observaci n microsc pica de c lulas y tejidos fijados en el portaobjetos utili
88. rancier 3 Controleer de ingangsspanning De ingangsspanning is op twee plaatsen aan de achterzijde van de microscoop vermeld op het typeplaatje en boven de wisselspanningsaansluiting Controleer of deze ingangsspanning overeenkomen met de netspanning ter plaatse Raadpleeg de onderstaande instructies wanneer dat niet het geval is Door het gebruik van de microscoop met een afwijkende ingangsspanning zal het apparaat oververhit worden waardoor brand of ernstige schade aan de microscoop kan ontstaan Als de werkspanning op het typeplaatje afwijkt gt Steek de stekker van de microscoop niet in het stopcontact Neem contact op met de dichtstbijzijnde Nikon vertegenwoordiging Als de werkspanning boven de wisselspanningsaansluiting afwijkt gt Raadpleeg pag 68 en wijzig de ingangsspanninginstelling voordat u de netvoedingscahelaar inschakelt Voor de microscoop met op het typeplaatje de waarden 100 110 120 V De werkspanning kan worden ingesteld op AC 100 V 110 V of 120 V Voor de microscoop met op het typeplaatje de waarden 220 230 240 V De werkspanning kan worden ingesteld op AC 220 V 230 V of 240 V ce es N Nominale ingangsspanning 4 Gebruik de voorgeschreven lamp zekering en netsnoer Gebruik de voorgeschreven lamp en zekering Gebruik het meegeleverde netsnoer Door gebruik van een verkeerde lamp zekering of netsnoer kan het instrument beschadigen of brand ontstaan zie ook pag 8
89. reparaat zelf nooit aan Reinig wanneer het preparaat onverhoopt toch op de microscoop terechtkomt het verontreinigde deel volgens de standaardprocedure van uw laboratorium Aver oP 1 Schakel het apparaat uit voordat u de microscoop demonteert de lamp of de zekering gaat vervangen of de stekker van het netsnoer in het stopcontact steekt of daaruit verwijdert Schakel om elektrische schokken of brand te voorkomen altijd de microscoop uit voordat u de stekker uit het stopcontact haalt Schakel ook eerst de microscoop uit en haal de stekker uit het stopcontact voordat u de microscoop monteert of de lamp of de zekering gaat vervangen Zet de netvoedingsschakelaar in de stand O om het apparaat uit te schakelen 2 Zorg ervoor dat er geen vocht of voorwerpen in de microscoop terecht kunnen komen Voorkom dat vocht in de microscoop terecht kan komen waardoor kortsluiting zou kunnen ontstaan en oververhitting of andere storingen zouden kunnen ontstaan Schakel wanneer er onverhoopt vloeistof in het instrument terechtkomt onmiddellijk de netvoedingschakelaar in de stand O en haal de stekker uit het stopcontact Dep het oppervlak vervolgens op met een droge doek Kortsluiting kan ook ontstaan doordat er voorwerpen in de microscoop terechtkomen Gebruik de microscoop niet als er vaste stof of vloeistof in de microscoop terechtgekomen is en neem contact op met de dichtstbijzijnde Nikon vertegenwoordiging 3 Preparaatbevestingsplaat T
90. rse of operation please recheck the symptoms referring to the tables below before contacting your nearest Nikon representative Optical Darkness at the periphery no viewfield seen or uneven viewfield brightness Causes Corrective Measures Revolving nosepiece not in click stop position objective not centered in the optical path objective correctly into the optical path gt Revolve to click stop position swing the If the microscope is equipped with a field diaphragm correct positioning so that the field diaphragm image is Condenser position too low D focused on the specimen surface P 57 If the microscope is not equipped with a field diaphragm position the condenser slightly lower than the upper limit Condenser not installed correctly Install correctly P 70 microscope is equipped with a field Center the field diaphragm P 57 diaphragm Field diaphragm not centered if the Field diaphragm closed too far if the microscope is equipped with a field Open properly P 60 Install correctly P 72 gt diaphragm p Field lens unit not installed correctly Dirt or dust on the lens condenser objective field lens eyepiece gt Clean the lens P 84 specimen 77 Chapter 8 Troubleshooting Tables 1 Optical Dirt or dust in the viewfield Causes Condenser position too low Aperture diaphragm closed too far Dirt or dust on the lens condenser objective
91. s de la lentille de champ la lampe est plac e sous la lentille de champ vous rappelle ce qui suit e La lampe et ses environs lentille de champ y compris deviennent tr s chauds pendant et imm diatement apr s l illumination e Risque de br lures Ne touchez pas la lampe ou ses environs pendant et imm diatement apr s l illumination e V rifiez que la lampe et ses environs sont suffisamment refroidis avant d essayer de remplacer l ampoule Attention Cette tiquette de symbole plac e pr s de l entr e CA vous rappelle ce qui suit e V rifiez la tension d entr e avant de mettre le microscope sous tension La tension d entr e est indiqu e sur la plaque du fabricant et au dessus de l entr e CA e Si la tension d entr e indiqu e diff re du niveau de tension local ne mettez pas le microscope sous tension Proc dez comme suit la place Tension diff rente sur la plaque du fabricant Contactez l agent Nikon le plus proche Tension diff rente au dessus de l entr e CA Modifiez le r glage de tension d entr e voir la page 68 13 French Safety Precautions ZN AVERTISSEMENT 1 Emploi pr vu pour le produit Ce microscope est principalement pr vu pour l observation microscopique de cellules et tissus fix s sur une lame porte objet par illumination diascopique transmise et piscopique r fl chie Il est con u essentiellement pour l exp rimentation et l observation dans le
92. s h pitaux ou laboratoires de telles cellules et tissus dans les domaines de la pathologie et de la cytologie 2 Nele d montez pas Le d montage peut provoquer un dysfonctionnement et ou une d charge lectrique et conduira la perte par d ch ance de tout droit sous garantie Ne d montez aucune pi ce autre que celles indiqu es dans ce mode d emploi En cas de probleme avec le microscope adressez vous l agent Nikon le plus proche 3 V rifiez la tension d entr e La tension d entr e est indiqu e deux emplacements l arri re du microscope sur la plaque du fabricant et au dessus de l entr e CA Confirmez que ces indications de tension correspondent la tension fournie dans votre r gion Si ce n est pas le cas suivez les instructions ci dessous L emploi d un microscope indication de tension d entr e diff rente provoquera une surtension ou la surchauffe ce qui pourra entra ner un incendie ou des dommages graves pour le microscope Si l indication de tension sur la plaque du fabricant diff re gt Ne branchez pas le microscope Contactez l agent Nikon le plus proche Si l indication de tension au dessus de l entr e CA diff re gt Consultez la page 68 pour modifier le r glage de tension d entr e avant de mettre sous tension Pour un microscope plaque du fabricant indiquant 100 110 120 V Lua Indications de La tension est r glable 100 V 110 V ou 120 V CA Eh tension d entr e
93. se increases the brightness turning the dial counterclockwise decreases it 2 Interpupillary Distance Adjustment Adjust the distance between the eyepieces to merge the right and left viewfields into one This is an adjustment to match the distance between eyepieces with the distance between your eyes Merge the right and left viewfields into one Changing the Eye Level Turn the binocular part of your microscope 180 degrees and you will get the microscope with higher eye level If you feel uncomfortable in observing the image due to its size you may find When the interpupillary distance is 64 mm this convenient the eye level is raised about 30 mm There is one condition however that should be met Before returning the microscope in the cabinet put back the binocular part to its original low position Since left high the tip of the eyepiece will become the highest point of the microscope and may be damaged easily when bumped against the shelf In addition the microscope may be higher than the shelf The eye level can be raised even higher till 50 mm if an Eye level raiser optional is installed between the basic unit and the eyepiece tube See p 65 for details on the eye level raiser 54 Chapter 4 Microscopy Detailed Procedure Align the Diopter Ring with the Engraved Base Line Turn the diopter ring on the right eyepiece to align its bottom edge with the engraved base lin
94. should be connected to PE protective earth terminal e Approved according to EU EN standards 3 conductor grounding Type HO5VV F 3 m long maximum rated at 250 V AC minimum Room Temperature 0 to 40 C Relative Humidity 85 Max No condensation should form Altitude 2000 m Max Pollution Degree 2 Installation Category Overvoltage Category Category 2 For indoor use only 9 Storage and Transport Environmental Conditions Temperature 20 to 60 C Humidity max 90 RH non condensing 10 Conforming Standards The model for 100 110 and 120 V areas is a UL listed product The model for 220 230 and 240 V areas satisfies the EU IVD Directive In vitro diagnostic medical device directive GM approved product In vitro diagnostic medical device The model for 220 230 and 240 V areas satisfies the EU LV Directive Low Voltage Directive The model for 220 230 and 240 V areas satisfies the EU EMC Directive EN 61326 The model for 100 110 and 120 V areas is not covered by the FCC 86
95. slide utilizando ilumina o diasc pica transmitida e episc pica reflectida A sua principal finalidade a experimenta o e a observa o em hospitais ou outros laborat rios de tais c lulas e tecidos nos campos da patologia e citologia 2 N o desmontar A desmontagem pode provocar avarias e ou choques el ctricos resultando na anula o de todas as reclama es ao abrigo da respectiva garantia N o desmonte nenhuma pe a salvo as descritas no presente manual Se experimentar algum problema com o microsc pio participe o facto ao representante mais pr ximo da Nikon 3 Verifique a tens o de entrada A tens o de entrada est indicada em dois locais na parte posterior do microsc pio na placa de tipo e acima da entrada de AC Confirme se estas indica es da tens o de entrada correspondem tens o da regi o em que se encontra Caso contr rio siga uma das seguintes instru es A utiliza o de microsc pios com indica es de tens o de entrada diferentes provocar sobretens es e sobreaquecimentos resultando da inc ndios e danos graves aos referidos microsc pios e Se a indica o de tens o na placa de tipo for diferente gt N o ligue o microsc pio Contacte o representante da Nikon mais pr ximo e Se a indica o de tens o acima da entrada AC for diferente gt Consulte a p g 68 e altere a defini o da tens o de entrada antes de ligar o interruptor Se o microsc pio apresentar uma pla
96. t could result in discoloration of the plastic parts MEN When Not in Use When the microscope is not in use cover with the vinyl dust cover and store it in a dry place where mold is not likely to form e Make sure that the power switch is off turned to O and the lamp is cool enough to touch before covering with the vinyl dust cover We especially recommend that the objectives and eyepieces be kept in a container such as a desiccator with desiccant in it Periodical Inspections maintain the performance the microscope periodical inspections and maintenance are recommended e For details contact your nearest Nikon representative 84 1 2 3 4 5 6 7 Technical Specifications Model Name Dimension and Weight Optical System Focusing Mechanism Stage Revolving Nosepiece Electrical Specifications Lamp Rating Average lamp lifetime e Output Rating e Input Rating ECLIPSE E200 Microscope basic unit 227 W x382 D x415 H mm 10Kg CF infinity corrected optical system Second objective focal length f 200 mm Built in diascopic illumination system Simplified Kohler s illumination system Fine focus knob graduation Fine focus knob travel Coarse focus knob travel Stage vertical movable range 2um graduation 0 2 mm up or down revolution about 37 7 mm up or down revolution 1 5 mm upward and 25 mm do
97. tesso Se l indicazione della tensione sulla targhetta con la sigla diversa gt Non collegare il microscopio alla presa di rete Contattare il rappresentante Nikon pi vicino Se l indicazione della tensione sopra l ingresso in c a diversa gt Prima di accendere l interruttore dell alimentazione vedere a p 68 e cambiare l impostazione della tensione in ingresso Per il microscopio con la targhetta della sigla che indica 100 110 120 V possibile impostare la tensione a 100 o 120 Vin c a Per il microscopio con la targhetta della sigla che indica 220 230 240 V possibile impostare la tensione a 220 230 o 240 V in c a PE ao Indicazioni della tensione 5 in ingresso NS 4 Utilizzare la lampada il fusibile e il cavo di alimentazione specificati Utilizzare la lampada e il fusibile specificati Utilizzare il cavo di alimentazione in dotazione L uso di una lampada di un fusibile o di un cavo di alimentazione non corretti pu danneggiare lo strumento o provocare incendi Per quanto riguarda il cavo di alimentazione vedere anche a p 85 Se si utilizza una prolunga utilizzare solo un cavo che include un filo protettivo di messa a terra PE Lampada specificata Lampada alogena da 6 V 20 W 7388 PHILIPS o HLX64250 OSRAM o Lampada alogena da 6 V 30 W 5761 PHILIPS e Fusibile specificato 250 V 1 A tipo con ritardo temporale a basso grado di rottura 2 fusibili miniaturizzati da
98. the coverglass when a specimen image is sharply focused Generally the higher the magnifying power of the objective the shorter the working distance 75 Chapter 7 Optical Characteristics 5 6 7 2 Microscope Terminology Field Number of the Eyepiece The diameter of the opening of the fieldstop inside the eyepiece measured in mm When an eyepiece has an indication of 10x 20 it means that the magnification is 10x and the field number is 20 for that eyepiece Real Viewfield The diameter in mm of the field of view observable through the eyepiece Real viewfield field number of eyepiece magnification of objective Depth of Focus The depth thickness of the specimen image in focus extending above and below the focused image plane The larger the N A of the objective the shallower the depth of focus Depth of focus um x1000 n 2 x N A 7xMx Above is an approximation assuming the resolving power of an eye as 2 minutes is the wavelength of light used The depth of focus in the table on P 75 is indicated for 0 55um n is the refractive index of a medium between the objective lens and the specimen or condenser n 1 when the medium is air and n about 1 5 when the medium is oil M is the total magnification the individual magnifying power of the objective x that of the eyepiece 76 Troubleshooting Tables If difficulties should be encountered in the cou
99. the nameplate showing 220 230 240 V The voltage can be set to AC 220 V 230 V or 240 V e Changing the Voltage Setting 1 Turn off the power switch turn to O and unplug the power cord 2 Remove the fuse holder using the flatblade screwdriver Voltage Selector Go Sm o 3 Remove two fuses and the voltage selector fe from the fuse holder AN 4 Attach the voltage selector to the fuse Fuse Holder Ce holder so that the indication of the voltage 7 Window provided in your region appears in the window of the fuse holder 5 Put the fuses and the fuse holder back in place 68 Chapter 6 Assembly 1 Assembly of Standard Set Removal of Shipping Clamps The stage top plate and focusing mechanism are clamped for protection against vibration and shocks during transportation Remove these shipping clamps with the hexagonal wrench provided e Stage Top Plate The top plate of the stage is retained in the Y axis direction by a plate fastened with two bolts Remove the bolts and the plate Focusing Mechanism Remove the field lens unit to expose a plate retaining the vertical movement of the focusing mechanism The plate is fastened with 3 bolts Remove the bolts and the plate Installing the Eyepiece Tube Loosen the eyepiece tube clamp screw by hand and place the eyepiece tube on the circular dovetail mount Tighten up the eyepiece tube clamp screw by hand Eyepiece Tube Clamp Screw
100. turned on and the lamp lights When turned to O power is turned off and the lamp goes off 15 Brightness control dial When turned clockwise the lamp voltage increases and the viewfield becomes brighter When turned counterclockwise the lamp voltage decreases and the viewfield becomes darker 16 Condenser focus knob Use this knob when focusing the field diaphragm image on the specimen p 57 The condenser focus knob is located on the opposite side of the stage motion knobs 17 Fine focus knob 18 Coarse focus Knob There are both coarse and fine focus knobs on the opposite side of the stage motion knobs 19 Coarse focus knob torque adjustment ring Used to adjust the tension torque of the coarse focus knob p 64 20 Nameplate Indicates the input voltage 21 Voltage selector Fuse holder Use this selector to match the input voltage of the microscope to the voltage provided in your region p 68 22 AC Inlet Plug the power cord into this inlet Make sure that the power switch is off turned to O before plugging the cord in Chapter 2 Switches and Controls 3 Stage 4 Specimen holder 12 Field diaphragm centering screws 5 Condenser aperture diaphragm 14 Power switch 15 Brightness control dial diaphragm 9 Longitudinal stage Zz motion Y Axis knob al ee 10 Lateral stage Sr motion X Axis knob 13 Fine focus knob 8 Blue filter
101. ube The eyepiece tubes for the ECLIPSE E400 and E600 can be also used for the E200 Distribution of Light for Trinocular Eyepiece Tube Example Vertical Tube Adapters When installing the photomicrographic equipment and TV camera on the vertical tube of the trinocular eyepiece tube vertical tube adapters are required in between For details ask your nearest Nikon representative Typical combinations are shown below e Photomicrographic equipment Use the TV vertical tube and the photomicrographic vertical tube adapter C mount CCTV camera Use the TV vertical tube and the direct C mount adapter The relay magnification is 1x e Digital still camera Use the TV vertical tube and the direct C mount adapter Use the relay lens for the C mount adapter as necessary 65 Chapter 5 Miscellaneous Operations 6 ND Filter for the Objective O TV Relay Lenses For the C mount CCTV camera 0 6x 0 45x and 0 35x relay lenses are available Select the magnification of the relay lens according to the size of the photographic element When a 3CCD TV camera is used with some relay lenses the colors of the video image may blur For details ask your nearest Nikon representative O Color Tone The color tone of the light from the lamp varies with the position of the brightness control dial If the dial is turned clockwise and the voltage is increased the light has a bluish tone If the voltage is reduced the light has a reddish t
102. ures Specimen holder not securely fastened i y gt Fasten securely P 71 to the stage Binocular images not integrated Causes Corrective Measures Interpupillary distance not adjusted Adjust P 54 correctly Diopter not adjusted correctly gt Adjust P 57 Excessive eye fatigue Causes Corrective Measures Inte illary distance not adjusted rpupillary di Ju Adjust P 54 correctly Diopter not adjusted correctly Adjust P 57 Adjust brightness using the control dial Inadequate brightness or illumination P 54 vv 82 Chapter 8 Troubleshooting Tables 3 Electrical Problems Electrical Problems Lamp does not light when switched on Causes Corrective Measures No electrical power Check power cord connection P 69 Lamp bulb not inserted Insert correctly P 72 Lamp bulb burnt out Replace bulb P 72 Incorrect lamp used Use the specified lamp P 72 gt gt Fuse blown out Replace fuse P 73 Flickering or unstable lamp brightness Causes Corrective Measures Lamp bulb about to fail p Replace bulb P 72 Power cord not correctly connected p Connect correctly Bulb not correctly inserted into socket gt Insert correctly P 72 Sudden lamp failure Causes Corrective Measures Incorrect lamp used gt Use the specified lamp P 72 Select the voltage that corresponds to Incorrect input voltage aly the voltage provided in your region
103. urvallisuusohjeet on osoitettu alla olevilla symboleilla Noudata n iden symbolien osoittamia ohjeita varmistaaksesi oikean ja turvallisen k yt n Symboli Merkitys A varorrus T ll symbolilla merkittyjen ohjeiden huomiotta j tt minen voi johtaa kuolemaan tai vakavaan loukkaantumiseen AN VAROTOIMET T ll symbolilla merkittyjen ohjeiden huomiotta j tt minen voi johtaa loukkaantumiseen tai omaisuusvahinkoon Finnish 36 Safety Precautions Laitteistossa k ytettyjen symbolien merkitys Tuotteessa n kyv t symbolit ilmaisevat tarvetta varotoimiin k yt n kaikissa vaiheissa Tarkista aina k sikirjasta ja lue asiaankuuluvat ohjeet ennen kuin k sittelet osaa johon symbolit on oheistettu Symboli Merkitys A Varoitus Biologinen vaaratekij T m symbolietiketti kiinnitettyn telineeseen muistuttaa sinua seuraavasta e VAROITUS N ytteen ja mikroskoopin kontakti voi aiheuttaa biologisen vaaratekij n e V ltt ksesi biologisesti vaarallisen kontaminaation l kosketa kontaminoitunutta aluetta paljain k sin e Dekontaminoi kontaminoitunut osa laboratorion normaalien menettelytapojen mukaisesti A Varoitus kuumuudesta T m symboli kiinnitettyn l helle kentt linssiyksikk lamppu on sijoitettu kentt linssiyksik n alle muistuttaa sinua seuraavasta e Lamppu ja sit ymp r iv alue mukaan lukien kentt linssiyksikk kuumenee hyvin kuumaksi k yt n aikan
104. us oil could flow out onto the stage and the condenser and degrade performance After completing oil immersion observation be sure to clean the objective condenser and any other parts that may be stained by oil Any oil residue left on the lenses of oil immersion type objectives or adhesion of oils on the front lens of dry type objectives will degrade image quality Use petroleum benzine to wipe off oil and finish with absolute alcohol ethyl or methyl alcohol If petroleum benzine is not available use methyl alcohol instead In that case wipe off the oil several times generally 3 or 4 times as the detergency of methyl alcohol is weaker than petroleum benzine ZN warnine When handling petroleum benzine and absolute alcohol be sure to follow the instructions provided by the manufacturers Since they are highly flammable take great care when handling them O Cautions on Handling the Immersion Oil e Close the container cap tightly after use Make sure that the cap is closed tight after refilling the container Check the cap periodically to make sure it has not come loose allowing oil to leak out e Do not press the container hard Oil could splash out e If you find an oil drips around the container wipe them off e Avoid contact of immersion oil with eyes or skin In the event of contact with eyes or skin take one of the following measures although Nikon immersion oil does not contain any toxic ingredients 0
105. wnward from the focal plane Stroke X axis 77 mm Y axis 53 mm 4 hole fixed type Halogen lamp 6V 20W PHILIPS 7388 or OSRAM HLX 64250 or Halogen lamp 6V 30W PHILIPS 5761 Halogen lamp 6V 20W 100hrs Halogen lamp 6V 30W 100hrs 6V 5A max Model for 100 110 and 120 V Areas e Input voltage Select from 100 V 110 V or 120 V AC by relocating the fuse holer in the AC inlet e Frequency 50 60 Hz e Voltage fluctuation 10 e Rated current 0 8 A max e Fuse rating 250 V 1A time lag low breaking type 5x20 miniature fuse x2 e Power Cord Use only the following power supply cord Using the wrong power cord could result in danger or fire The protection Class I equipment should be connected to PE protective earth terminal e UL listed detachable power cord set 3 conductor grounding type SVT No 18 AWG 3 m long maximum rated at 125 V AC minimum 85 Chapter 10 Technical Specifications Model for 220 230 240 V Areas e Input voltage e Frequency e Voltage fluctuation e Rated current e Fuse rating Power Cord Protection Class Class I 8 Operating Environment Select from 220 V 230 V or 240 V AC by relocating the fuse holder in the AC inlet 50 60 Hz 10 0 4 A max 250 V 1A time lag low breaking type 5x20 miniature fuse x2 Use only the following power supply cord Using the wrong power cord could result in danger or fire The protection Class I equipment
106. y Detailed Procedure 6 Eyepiece Diopter Adjustments D 9 Adjust the diopter ring on the eyepieces according to the difference between your left and right See 40x eyesight This adjustment enables the user to take full advantage of the high quality objectives Focus with including their parfocality this knob 1 Swing the 40x objective in the optical gt path Rotate the coarse and then fine focus knobs to bring the specimen in focus 2 Switch back to the 10x or 4x objective While looking into the right eyepiece And focus with with your right eye focus on the this ring specimen by rotating the right diopter e TI N ring and not using the focus knob 4 AN 3 While looking into the left eyepiece with A A your left eye focus on the specimen by Use your rotating the left diopter ring and not left eye 10 x N using the focus knob A 4 Repeat the steps to O The next procedure is for microscopes equipped Q Then focus with field diaphragm If the microscope does 6 with this ring not have a field diaphragm confirm the position E JA of the condenser and skip to procedure 8 The A gt condenser should be a little lower than its Use your 10 X AE highest position right eye Sa 7 Field Diaphragm Centering and Focusing for microscopes with the field diaphragm 10x The field diaphragm is used to limit the specimen s illuminated area to the size of the viewfield If S off centered its imag
107. z le proximit du produit pour toute r f rence ult rieure Dans ce mode d emploi les instructions concernant la s curit sont indiqu es par les symboles ci dessous Suivez bien les instructions marqu es par ces symboles pour assurer un fonctionnement correct et s r Symbole Signification ZN AVERTISSEMENT La n gligence des instructions marqu es de ce symbole peut entra ner le d c s ou des blessures graves LN attention La n gligence des instructions marqu es de ce symbole peut entra ner des blessures ou des d g ts mat riels French 12 Safety Precautions Signification des symboles utilis s pour cet appareil Le symbole apparaissant sur le produit indique le besoin de pr cautions tout moment pendant Vutilisation Reportez vous toujours au mode d emploi et lisez les instructions pertinentes avant de manipuler toute partie laquelle le symbole est attach Symbole Signification Attention Risque biologique Cette tiquette de symbole attach e sur le socle vous rappelle ce qui suit e AVERTISSEMENT Le contact d un chantillon et du microscope peut pr senter un risque biologique e Pour viter la contamination biologique ne touchez pas la partie contamin e les mains nues D contaminez la partie contamin e conform ment la proc dure standard appliqu e dans votre laboratoire Attention la chaleur Cette tiquette de symbole plac e pr
108. zando la iluminaci n diasc pica transmitida y episc pica reflejada Est dise ado para el objeto principal de experimentaci n y observaci n de c lulas y tejidos del rea de la patolog a y citolog a en los hospitales u otros laboratorios 2 No lo desarme El desarme puede causar desperfectos y o descargas el ctricas y quedar excluido del derecho a todos los reclamos contra la garant a No desarme ninguna parte fuera de lo que se describe en este manual En el caso de encontrar alg n problema en su microscopio se ruega notificar al representante de Nikon m s pr ximo 3 Verifique la tensi n de entrada La tensi n de entrada est indicada en dos lugares de la parte trasera del microscopio en la plaqueta de caracter sticas y sobre la entrada de CA Confirme que estas indicaciones de la tensi n de entrada correspondan a la tensi n suministrada en su regi n En el caso contrario siga una de las siguientes instrucciones El uso del microscopio con tensiones de entrada diferentes a las indicadas puede producir la sobrecorriente y el sobrecalentamiento que pueden causar el incendio o da os severos al microscopio e Cuando difiere la tensi n de la indicaci n de la plaqueta de caracter sticas 3 No conecte el microscopio Tome contacto con el representante Nikon m s pr ximo e Cuando difiere la tensi n de la indicaci n de tensi n sobr gt Vea la P g 68 y cambie el ajuste de la tensi n de entrada antes de conect

Download Pdf Manuals

Related Search

Related Contents

  Graco 7938 Stroller User Manual  WalkTop Owner`s Manual-CoMbinED copy  Acco 60035 flat panel desk mount  HOJA DE DATOS DE SEGURIDAD  Palo Handbuch, Basiswissen für PAlo OLAP Server  Bulletin FG400-01EN FG400 Series Arbitrary  CA Manual (portugese) 77155  Edelbrock 17931 Automobile Parts User Manual  User Manual  

Copyright © All rights reserved. Failed to retrieve file