AVA300 nmr (1D)user guide 20051211
Contents
1. hold left button to drag from peak left side to right side peak will be integrated Then click up right Ei to save 33 click will display DA Print Ctri P plot F x Options C Print active window prnt Print with layout start Plot Editor plot C Print with layout plot directly autoplot Required parameters LAYOUT josz comp Apply Use plot limits Fill data set list Sree lite eine ine ay ee from Zour defaut portfolio from Plot Editor Rese Actions as saved in Plot Editcr from portfolio saved in data set R Lance Help 34 LAYOUT for 1H choice 1D_H X WP or for 13C or 31P_ choice 1D_X XWP click OK into the xwinplot panel 35 change the spectrum range integration parameter etc make mouse arrow to the spectrum or parameter click left button then the spectrum parameter corner had 8 green spot at this moment click right button will show Edit set the plot spectrum range integrate preak level 1D 2D Edit set spectrum and integrate level 36 click amp to print out your spectrum 1 change sweep range before zg type sw to change the value 3 When you copy file to process by Topspin you need to create some sub folder lt dir gt data lt user gt nmr lt your dataset name gt then put the file to lt your dataset name gt spooler type qu display queue time edqu edit queue time create file Move to end of top Open file Hol2. AVA300 nmr 1D user guide 20130305 L 2 Note The magnetic object do not be put over the yellow line of magnet Do not push magnet A Login name 1 log on type user nmr1 2 click TOPSPIN 2 1 3 click open the BSMS panel B Put the sample 4 clean the tube and spinner inside and outside 5 put tube in spinner the tube height don t over 1 8 cm 6 click BSMS panel Main SAMPLE wait to change to green and hear the air voice 7 put the tube in magnet click Main SAMPLE to close the air C nmr 1 Create a new file 8 type ede don t use special character such as lore tee ri UT hh Don t use or at first word cclin kllu small letter Experiment Select the standard file 1 D_1H_qnp 1D_13C_dh_qnp 1D_19F_dh_qnp 1D_31P_dh_qnp ID_DEPT45_gqnp 1D_DEPT90_qnp 1D_DEPT135_qnp for av3 400 nmr st 1D_1H_bbfo st 1D 13C _ dh bbfo st 1D 31P_dh bbfo st 1D 19F_ dh_ bbfo st 2D COSY bbfo st 2D HMBC bbfo st 2D NOESY_ bbfo st 2D_TOCSY_ bbfo TITLE Set title Note or title file path D DATA USER NMR EXPNO 9 type ii 10 type rsh shim 1 or rshs qnp probe for av3 400 nmr 10 type rshs 11 click T or type lockdisp display lock panel 12 type lock Enter choice CDCL3 DMSO T OK p s If you can t lock sample click Main LOCK 13 click BSMS panel the Main SAMPLE check rate click Sample amp Level SAMPLE SPIN ratel meas
3. d left click withdraw then move up and down to change peak height as a switch Hz ppm unit eg copy Peak height 2 paste i Peak height 2 Phase mode Peak height 8 A reference mode it Peak een 8 E baseline correction mode T left click withdraw to move up and down change peak height peak picking mode Set peak height thi Integration mode Set range of spectrum Overlape spectrum Full spectrum te D move right Increase range of spectrum Hold left click withdraw to move right and left Reduce range of spectrum 7 Le e Spectrum move left Hold left click withdraw then move up and LS ee ke oe move to end of left Set edge of spectrum 5 Spectrum move to end of right Go back last range of spectrum m
4. ter for 13C or 31P_ type Ib Enter 3 Enter type ef Enter Phase auto phase correction 27 manual phase correction 28 27 type apk to auto phase correction 28 manual phase correction move mouse to click A gt into phase panel the window upper leftside has yellow color Ay move mouse to ol hold mouse to move up side down move mouse tol 1 _hold mouse to move up side down till the phase is correct Then click up right a to save Baseline Correction 29 type abs n Set Standard Peak 30 click oe into standard peak panel the window upper left corner has yellow color 4 p move the mouse to solvent peak click left button key in the chemical shift Set Minimum Intensity 31 click sh into intensity panel the window upper left corner has yellow color il when p is green move mouse to spectrum hold left button to drag make the square cover the peak then will show the peak picking or click then move the mouse to square edge hold left button to drag it will change the square area you also can make the many small square to cover the peak Then click EJ to save Integration 32 click I Into integrated panel the window upper left corner has yellow color I move Plot p s mouse to spectrum hold left button to drag from peak left side to right side to expand spectrum Then click U to let p change to green color when you move mouse to spectrum then
5. ure for av3 400 nmr 13 you don t need to click 2 Shimming 14 click Main SHIM click to change the level for every step as the crude or fine tune click Main VALUE till lock level line is uppermost Repeat to click Z7 Z if lock level line over the range of window use BSMS panel change Main LOCK or make lock level line is at upper 4 line for av3 400 nmr 14 type topshim 3 Acquisition 15 type getprosol 16 type rga type rg 17 type ns set the multiple of 4 18 type zg start run the experiment tr transfer part of dara for H type ft for 13C or 31P type lb 3 type ef 19 wait for zg finished or type halt to stop experiment Run C or P the same sample Repeat 8 9 15 19 for av3 400 nmr Repeat 8 9 type atma 15 19 D logout 20 type halt make sure the nmr experiment is already stop 21 click BSMS panel the upper Main LOCK gt Main SAMPLE x a check Sample amp Level SAMPLE SPIN 0 yxx b check lock level line display as sine wave for av3 400 nmr 21 click BSMS panel the upper Main LOCK 22 click Main SAMPLE take nmr tube out click Main SAMPLE 23 click your data file top right corner turn off your data file 24 click top right corner turn off Topspin interface you don t need to log off PC E Processing 25 click View Browser Pnael on off to find your own data 26 for 1H type ft En
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