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TRILUTION LH User's Guide

1. l _ To select multiple Methods in a sequential order hold SHIFT any arrow key simultaneously To select Methods in a random order hold CTRL and select multiple Methods with the mouse TRILUTION LH Software Version 2 0 User s Guide Method Builder b In the Select a path field complete the following 1 Click The Browse For Folder window displays 2 On the Browse For Folder window select a folder and then click OK 3 Click OK On completion of the Export operation the Method is saved in the specified folder with a LHME extension The TRILUTION information dialog box displays provided to rename or overwrite the Method or to skip the export operation for that Method only BJ Runon Q Export operation amp compketed Si fore rvormation The TRILUTION information dialog box c On the TRILUTION information dialog box complete the following To view the log information of the Export operation click DETAILS e Click OK TRILUTION LH Software Version 2 0 User s Guide If a Method with the same name is found in the export path an option is 3 9 spouja bunsodxy Importing Methods Method Builder Importing Methods 1 Onthe Method Builder click IMPORT The Select Method Exported Files window displays Select Method Exported Files Look in E Working My Network Fir name Example Method hme Places Fie
2. Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The speed at which the Result Volume moves into the probe For a range of 10 mL min Source Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Result Volume is drawn Source Zone Tray The well in the Source Zone from which the Result Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray An additional volume to the Result Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Result Volume Tray The quantity of the air gap aspirated before the Result Volume 20 uL Source Air Gap Tray The speed at which the Result Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Aliquot Properties Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide A 17 yonbily Aliquot Tasks Liquid Handling Aliquot Properties Continued A Property Name Brief Descr
3. Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source Volume is delivered When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Dispense Properties Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide A 59 su ds q Dispense Tasks Liquid Handling Property Name Liquid Level Following Dispense Advanced Rinsing Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option A Default Value Liquid Level Detection When selected the probe will stop when the instru
4. Application Manual Control 9 Multiple Probe 215 The Multiple Probe 215 Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons Multiple Probe 215 Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone to 1 which probe A will move Z Offset The distance up whena positive number 2mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom Multiple Probe 215 Manual Control Table Page 1 of 3 9 64 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control Multiple Probe 215 Manual Control Continued Property Name Brief Description Default Value Use X Enter anumber to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm l Click MOVE Z value Output Contacts 24V A filled box indicates that the circuit is Toggle the contacts open closed off 1 closed on An empty box indicates that on by selecting and clearing the 2 the circuit is open off boxes 3 4 Input Contacts A A filled box indicates that the circuit is Monitor status
5. Valve Position Rotates the valve to the selected Inject Click SWITCH position Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position ASPEC XL4 Manual Control Table Page 3 of 3 9 42 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control GX 271 ASPEC without Pump The GX 271 ASPEC without Pump Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons GX 271 ASPEC without Pump Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number 2mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom GX 271 ASPEC without Pump Manual Control Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 43 MOPpUIM qe O4 U0D jenuew Manual Control Tab Window Application Manual Control 9 GX 271 ASPEC without Pump Manual Control Continued Property Name Brief Description Default Value Use X Enter a nu
6. a id Brief Description Default Value Use Valve The number of ports in the VALVEMATE valve 2 Position Rotates the valve to the selected position 1 Click SWITCH Button HOME VALVE Rotates the valve to the position selected in the Configuration Sy Sy VALVEMATE II Manual Control 9 74 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control Manual Instrument Control For manual control to occur TRILUTION LH needs the Configuration and Bed Layout information set in the Method Use the following procedures to set which Method information will be used for manual control 1 Open an Application Select the Manual Control tab Select a Method from the drop down list of Methods in the open Application 4 Click Go The instruments will initialize TRILUTION LH Software Version 2 0 User s Guide 9 75 J01 U05 JUaWINAYSU enuey Working with Variables Variables accept run time values to perform actions TRILUTION LH Software Version 2 0 User s Guide 10 10 1 Variables Working with Variables 10 Variables A Variable is anamed container that accepts values for the properties specified at the Task level or Method level Variables can be defined for the following 10 2 Commands Loops Expressions Conditional Operators Unconditional Operators Tasks The New Variable Dialog TRILUTION LH Software Version 2 0 User s Guide Working with Variables
7. Click PRIME to start or STOP PRIME to end Prime Flow Rate 10 mL min The speed at which the reservoir fluid moves into and out of the syringe Buttons HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 402 Pump Single Manual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 9 23 MOPpUIM qe J01 Uu0 enuey Manual Control Tab Window Manual Control 9 Default Value Use Used when replacing a syringe Application 402 Pump Single Manual Control Continued Property Name Brief Description LOWER SYRINGE Descends the syringe s piston operating rod 25 of a full stroke as the syringe pump aspirates from the reservoir The valve will switch to the outlet position RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 402 Pump Single Manual Control Table Page 2 of 2 9 24 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 406 Pump Dual The 406 Pump Dual Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons Property Name Active Syringes 406 Pump Dual Manual Control Brief Description The syringes that will be used Default Value Left Syringe Right Syr
8. Menu Description Cuts the Command for deleting or pasting in a new CUT location in the workspace in an operator or expression or in another Task Copies the Command to duplicate in a new location in the COPY workspace in an operator or expression or in another Task pace Places a cut or copied Command in the workspace in an operator or expression or in another Task DELETE Removes the Task from the workspace LINE BREAK A toggle for moving the selected element after the selected element to the next line below the selected element ______ ns th mmand proper for the sel SRGRERTIES Opens the Command property page for the selected Command TRILUTION LH Software Version 2 0 User s Guide Task Builder Task Right click on a Task icon to display the following menu options Menu Description Cuts the Task for deleting or pasting in a new location in CUT the workspace in an operator or expression or in another Task Copies the Task to duplicate in a new location in the COPY workspace in an operator or expression or in another Task Sete Places a cut or copied Task in the workspace in an operator or expression or in another Task DELETE Removes the Task from the workspace LINEBREAK A toggle for moving Tasks after the selected Task to the next line below the selected Task PROPERTIES Opens the task property page for the selected Task OPEN TASK Opens the Task TRILUTION LH Soft
9. Deliver with Tips amp This task dispenses liquid through the probe Tweaks Tasks Table Page 1 of 2 E 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks E Tweaks Tasks Move To This task moves the probe to the top home specified XY Coordinates or specified Z Coordinate Move To Zone This Task moves the probe to a specified height from bottom in a specified well in a specified zone SS Aspirate This Task aspirates liquid from the tray SS Deliver This Task dispenses liquid through the probe Tweaks Tasks Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide E 3 Aspirate Tasks Tweaks Aspirate This task aspirates liquid from the tray Properties Finstruments j ON p Source i Probe Source Volume fut p Source Flow Rate ml jmina 5 Eguilibeation Time min Property Name Source Volume Aspirate Properties Table Page 1 of 2 For more information about the properties on each tab see Aspirate Properties on page E 4 Aspirate Instruments on page E 5 Aspirate Sequence of Steps on page E 6 Aspirate Properties E 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Aspirate Properties Continued Property Name Brief Description Default Value Source Flow The speed at which the Source Volume moves into the probe For a range of 5
10. GX 271 Liquid Handler Properties Table Page 1 of 4 125 mm Probe 175 mm Probe TRILUTION LH Software Version 2 0 User s Guide 4 51 uoleinbyuos e Nurze Creating a Configuration Method Builder Configuration GX 271 Liquid Handler Properties Continued Property Name Brief Description Default Value Range Z Clamp Height mm This number corresponds to where 175 Calculated based on the mounting clamp is on the Z Clamp Height and Probe vertical ruler on the installed Z arm Type Range provided on screen Z Safe Height mm The Z height to which the 172 Calculated based on instrument s probe moves before Z Clamp Height and Probe moving in the X or Y direction Type Range provided on screen Transfer Tubing mL The size of the tubing being used 5 0 0 5 1 1 5 0 10 5 30 50 from pump to probe Other Tubing mL When selected the non standard 0 0 99 size of the tubing being used from pump to probe GX Direct Inject Valve Indicate the size of the valve Not Installed Not Installed installed 1 8 1 16 Waste Indicate the solvent valve port to be Port 1 Port 1 Port 2 Port 3 Port 4 used for waste GX 271 Liquid Handler Properties Table Page 2 of 4 Port 5 Port 6 4 52 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 271 Liquid Handler Properties Continued Property Name Brief Description Default Value
11. is entered or down when a negative number is entered from the Tube Bottom GX 271 Liquid Handler without Pump Manual Control Table Page 1 of 3 9 50 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 GX 271 Liquid Handler without Pump Manual Control Continued Property Name Brief Description Default Value Use X Enter a number to move to a position to 0 0 mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm Click MOVE Z value Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the the circuit is open off boxes 24V Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the the circuit is open off boxes Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the circuit is open GX 271 Liquid Handler without Pump Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 51 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual
12. Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Transfer Outside Rinse Table Page 2 of 4 0 mm B 152 TRILUTION LH Software Version 2 0 User s Gui
13. GSIOC ID A unique numerical address that must be 0 0 31 known to the instrument and to the software that allows the software to communicate to the instrument 444 QuadDilutor Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 33 uoleinbyuo e Nurze Creating a Configuration Method Builder Configuration 4 506C System Interface The 506C System Interface Properties Window Properties 506C System Interface Properties Default Property Name Brief Description Value Range Contacts Output 1 Optionally type a description for the contact This description will be used to N A N A Output 2 identify the contact in a Task Output 3 Output 4 Output 5 Output 6 506C System Interface Properties Table Page 1 of 2 4 34 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 506C System Interface Properties Continued ee Default Property Name Brief Description Value Range Input A Optionally type a description for the contact This description will be used to N A N A Input B identify the contact in a Task Input C Input D Analog Input A Analog Input B Analog Input C Analog Input D General Instrument Name The name that will be used to identify this instrument in a Task Contact N A GSIOC ID A unique numerical address that must be known to the instrument and tothe 63 0 63 software that
14. LH Software Overview Lock The Lock option allows you to manually lock out the system All open unsaved Tasks or Methods will remain open Only that user or a member of the default Admin group can logon after a lockout S Contlirm Lock click On the Lock button the TRILUTION LH Applic to lock aton The Confirm Lock window TRILUTION LH Software Version 2 0 User s Guide 1 33 SNU W H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 To lock TRILUTION LH operations 1 On the main menu click the Lock button The Confirm Lock window is displayed 2 On the Confirm Lock window click Lock The Unlock TRILUTION LH window is displayed Unlock TRILLION LH The Unlock TRILUTION LH window To unlock TRILUTION LH operations On the Unlock TRILUTION LH window in the Password field type the password 1 34 TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Closing TRILUTION LH To close TRILUTION LH Click amp on the main menu TRILUTION LH Software Version 2 0 User s Guide 1 35 H7 NOILNTIYL Buso Task Builder 2 Tasks are combined Commands and Operators used to perform an action on a specific instrument and serve as one of the building blocks that make up a Method The drag and drop feature provides the ability to create Tasks easily by dragging the Commands Tasks Variables Operators and Expressions from the side palette and d
15. Property Name Brief Description Default Value Source Select Reservoir or Tray Reservoir Reservoir Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Reservoir The quantity of a sample O uL Source Volume Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn 1 Source Well Tray Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray The quantity of a sample O uL Source Volume Tray An additional volume to the Solution Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume SS Add Properties Table Page 1 of 3 B 6 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Add Properties Continued Property Name Brief Description Default Value Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rat
16. TRILUTION LH Software Overview TRILUTION LH Menus TRILUTION LH has two main menus Liquid Handling and Administrative Tools Click the Main Menu icon in the lower right corner of any builder to bring forward the main menu A description of these menus and submenus are as follows Liquid Handling Menu EMT TY MEV TE TTT ee TRILUTION LH hese einen LIOUIG HANDLING SOFTWARE 4 Application Chud Handling Method Builder Custom Task Bulider UUNes Reports About 00 QIW DA Gaw pe iimis t Use the options in this menu to create organize and run your application TRILUTION LH Software Version 2 0 User s Guide 1 11 SNU W H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 1 12 Menu Described on Application page 1 12 Method Builder page 1 12 Custom Task Builder page 1 12 Utilities page 1 13 Reports page 1 18 About page 1 18 Application This menu allows you to create a Sample List and run an Application Method Builder The Method Builder provides the ability to specify method conditions for controlling instruments Custom Task Builder The Custom Task Builder provides the ability to create modify delete export and import tasks A task is an action to be performed on a specific instrument and serves as a building block for a Method Tasks are a combination of Commands and Operators TRILUTION LH Software
17. The graphic below depicts the use of permissions for a more detailed workflow How User Permissions Integrate with an Extended TRILUTION LH Workflow Custom Task Builder Method Builder TRILUTION LH Software Version 2 0 User s Guide sdno19 pue siasp GSIOC Configuration Editor J The GSIOC Configuration Editor enables you to modify COM serial communications port and baud rate information Or you can use this editor if incorrect information appears in the GSIOC Utility window TRILUTION LH Software Version 2 0 User s Guide J 1 GSIOC Configuration Editor GSIOC Configuration Editor GSIOC Configuration Editor 1 Locate the GSIOC Configuration Editor GSCONFIG EXE using Windows Explorer or the shortcut at Start Programs Gilson Applications utilities GSIOC Configuration Editor During installation this editor was stored to C GILSON UTIL unless the installation path was changed Start the editor The GSIOC Configuration Editor window appears xj r Pol Setings GSIUC Server In the Port box indicate the computer s serial communications port COM port to which the Gilson interface instrument such as the liquid handler or 506C System Interface is connected Click 19200 or 9600 to select the baud The baud is the rate of data transmission between the computer and the Gilson instrument Click OK to save the changes A message box appears indicating that the computer must be restarted
18. 2 18 If a Task s or any Task s placed within a Task with the same name is found an option is provided to rename use the existing file or skip the Import operation for that Task c On the TRILUTION information dialog box complete the following To view import operation log information click DETAILS e Click OK TRILUTION LH Software Version 2 0 User s Guide Task Builder 2 Deleting Tasks 1 On the Tasks palette do one of the following Select a Task and right click to view the submenu On the submenu click DELETE Open a Task and on the action bar click DELETE A delete confirmation dialog box displays Gilson supplied tasks cannot be deleted TRILUTION Are you sure you want to delete the Task New l Delete All Version Yes The Delete Task dialog box 2 Onthe delete confirmation dialog box choose whether to delete only the current version or all versions of the Task The default is to delete only the current version of the Task To delete all versions of the Task select the Delete All Version check box 3 Click YES The Task is deleted TRILUTION LH Software Version 2 0 User s Guide sse Huljajag Closing the Task Builder Task Builder 2 Closing the Task Builder Close Open Tasks To close the Task that is open in the workspace click CLOSE Close All Open Tasks To close all open Tasks but leave the Task Builder open click CLOSE ALL Close the Task Builder To
19. GX 271 ASPEC without Pump Properties Property Name Brief Description Default Value Range Setup Probe Type mm If the stop pin was inserted in S1 select 125 mm Probe 56 mm Probe 56 mm Probe 125 mm Probe If the stop pin was inserted in S2 select 17S MA RTODE 125 mm Probe If no stop pin was used select 175 mm Probe Z Clamp Height mm This number corresponds to where the 185 Calculated based on mounting clamp is on the vertical ruler Z Clamp Height and on the installed Z arm Probe Type Range provided on screen Z Safe Height mm The Z height to which the instrument s 185 Calculated based on probe moves before moving in the X or Z Clamp Height and Y direction Probe Type Range provided on screen GX Direct Inject Valve Indicate the size of the valve installed Not Installed Not Installed 1 8 1 16 GX 271 ASPEC without Pump Properties Table Page 1 of 3 4 48 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 271 ASPEC without Pump Properties Continued Property Name Brief Description Default Value Range Contacts Output 1 Optionally type a description for the N A N A Output 2 contact This description will be used to 24V Output 1 identify the contact in a Task a Additionally indicate whether the contact should open close or remain in the same state on error The default is No Change for Outputs 1 and 2 and O
20. ID matmonoes Inanmonop The Multiple Probe 215 Properties Window Properties Multiple Probe 215 Properties Property Name Brief Description Default Value Range Setup Multiple Probe 215 Liquid Handler Z Arm Type mm This is the height of the Z arm installed onthe 125 mm Z Arm 125 mm Z Arm liquid handler 175 mm Z Arm Z Clamp Height mm This number corresponds to where the 125 0 175 A r the vertical ruler on Range provided on screen Multiple Probe 215 Properties Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 75 uoleinbiyuo gt e Nurze Creating a Configuration Method Builder Property Name Z Safe Height mm Configuration Multiple Probe 215 Properties Continued Brief Description The Z height at which the instrument s probe moves to before moving in the X or Y direction Default Value 122 Range Calculated based on Z Clamp Height and Z Arm Type Range provided on screen Configuration The number of probes and syringes that will be used when selected as the Liquid Handler in a Task 8 Probe Configuration 4 Probe Configuration 8 Probe Configuration Setup Syringe Pump ID A unique identifier to address syringes onthe instrument A B C D E F G H A B C D E F G H Syringe Name A unique name to address syringes on the instrument A B C D E F G H A B C D E F G H Size uL
21. Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source Volume is delivered 1 Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 SS Dispense Random to Random Properties Table Page 1 of 2 B 90 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dispense Random to Random Properties Continued Property Name Brief Description Default Value Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well i
22. TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Derivatize Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at th
23. TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 Shake Off FALSE a Move Z to top Tray Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into Source Well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected mM A U N gt Move Z to top Transfer Port Aspirate Source Air Gap at Air Gap Flow Rate Move probe to well in Source Zone Lower probe to Source Height from Tube Bottom Set Transfer Port to On Aspirate Source Volume Extra Volume at Source Flow Rate U A U N gt TRILUTION LH Software Version 2 0 User s Guide uonpuo Condition Tasks SPE 6 Set Transfer Port to Off 7 Move Z to top DISPENSE Tray 1 Move probe to DEC Well in DEC Zone 2 Lower probe to Result Height from Tube Top 3 Dispense Source Volume at Result Flow Rate 4 Wait Equilibration Time 5 Move Z to top 6 If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume delivered 7 If SPE 215 Shake Off TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 Shake Off FALSE a Move Z to top 8 Move probe to well in Inside Rinse Zone 9 Lower probe to Inside Rinse Height from Tube Bottom 10 Dispense Extra V
24. The Variable window displays information for the variable Deleting variables 1 Click the Variable List icon E 2 Select a variable from the variable list and do one of the following Right click on the variable and choose DELETE from the submenu Click DELETE Press the DELETE key on the keyboard The selected Variable is deleted TRILUTION LH Software Version 2 0 User s Guide Generating Reports 11 The Reports menu is used to generate reports You can view and print reports directly or export them to rich text format rtf Adobe PDF pdf Microsoft Word doc or Microsoft Excel xls file types for further study and formatting TRILUTION LH Software Version 2 0 User s Guide 11 1 The Reports Window Generating Reports 11 2 11 The Reports Window To open a Report window On the Liquid Handling Menu click REPORTS The Reports window is displayed Ed Reports J The Reports Window TRILUTION LH Software Version 2 0 User s Guide Generating Reports 11 Reports Overview You can generate the following types of reports C 2 This report comprises three parts a brief summation of the task information name version description modified date and created date variables defined for the task Variables and the un timed sequential steps Steps Method This report comprises six parts a brief summation of the method information name version description cre
25. s Guide B 139 S qOld SUIY SS SS Rinse Probes Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Rinse Probes Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the Outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from its current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the pr
26. s Guide 2 3 sapjing yseL Task Builder Task Builder 2 4 Operators Palette The Operators palette lists the Operators Variables and Expressions Commands Palette The Commands palette lists the available Commands Workspace The workspace is used to build new or modify existing tasks The left panel provides the sequence The right panel contains the details for a selected operator or expression Commands are represented by the following icon E Tasks are represented by the following icon E Toolbar Provides quick access to some options available in the Task window To activate a tool click on its icon Undo Redo The Undo J and Redo buttons provide the ability to Undo and Redo the last action within the Task TRILUTION LH Software Version 2 0 User s Guide Task Builder Variable List Lists the available variables for the Task Click the J button to view the list of variables used in the Task Click NEW VARIABLE to add additional variables or highlight an existing variable and click MODIFY For more information about variables see Chapter 10 Working with Variables Back The Back button E provides the ability to return focus to the left panel in the workspace TRILUTION LH Software Version 2 0 User s Guide 2 5 sapjing seL Task Builder Task Builder 2 6 Right click menu Command Right click on a Command icon to display the following menu options
27. s Guide A 85 XIW Mix Tasks Liquid Handling 8 Wait Equilibration Time 9 Repeat 3 8 until Number of Mixes is complete 10 Perform a Touch Off X direction if selected and move Z to Z Safe Height 11 Move probe to well in Inside Rinse Zone 12 Lower probe into well to Inside Rinse Height 13 Dispense Air Gap at Flow Rate 14 Move Z to Z Safe Height MIX WITH AIR CON DON A WU N gt 9 Move Z to Z Safe Height Aspirate Air Gap at Flow Rate Move probe to Mix Well in Mix Zone Move Z to Z Safe Height Aspirate Mix Volume at Flow Rate Lower probe into well to Result Height Dispense Mix Volume at Flow Rate Wait Equilibration Time Repeat 4 8 until Number of Mixes is complete 10 Perform a Touch Off X direction if selected and move Z to Z Safe Height 11 Move probe to well in Inside Rinse Zone 12 Lower probe into well to Inside Rinse Height TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A 13 Dispense Air Gap at Flow Rate 14 Move Z to Z Safe Height INSIDE RINSE Move Z to Z Safe Height Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume delivered Move Z to Z Safe Height N OW A WU N gt OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into wel
28. 0 uL when the Outside Volume is 0 Dispense with Tips Advanced Rinsing Table Page 1 of 2 C 36 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Dispense with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Dispense with Tips Advanced Rinsing Table Page 2 of 2 Dispense with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Dispense with Tips Instruments TRILUTION LH Software Version 2 0 User s Guide C 37 sdi y m asusadsig Dispense with Tips Ta sks Disposable Tips Dispense with Tips Sequence of Steps The following is an overview for the seque
29. B closed An empty box indicates that the C circuit is open D Pump Prime Flow Rate The speed at which the reservoir fluid 10 mL min CiiciacniMEResiareOreror rined moves into and out of the syringes Multiple Probe 215 Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 65 MOpUIM qe J01 Uu0 enuey Manual Control Tab Window Application Manual Control Multiple Probe 215 Manual Control Continued Property Name Brief Description Default Value Use Buttons HOME XYZ Moves probes to the home position MOVE TO TOP Positions the probes at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probes to the home position HOME SYRINGE Homes the dilutor and sends the pistons to the upper position with the valves in the probe position LOWER SYRINGE Descends the syringes piston operating rods 25 of a full stroke as the syringe pump aspirates from the reservoir The valves will switch to the outlet position RAISE SYRINGE Homes the dilutor and sends the pistons to the upper position with the valves in the probe position Multiple Probe 215 Manual Control Table Page 3 of 3 Used when replacing a syringe 9 66 TRILUTION LH Software Version 2 0 User s Guide Application Quad Z 215 Manual Control The Quad Z 215 Manual Control Window W
30. Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Dispense Random to Random Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Dispense Random to Random Advanced Rinsing Continued A Property Name Brief Description Default Value Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low 1 setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is
31. Contact Error This error results when a contact is activated The valid mathematical operator is and the valid values are OPEN or CLOSED TRILUTION LH Software Version 2 0 User s Guide 7 5 Buljpuepy 10113 JUaWINAYSU dN Hulz a5 Setting Up Instrument Error Handling Method Builder Error Handling Error Responses Refer to the following table for information about how TRILUTION LH responds to an error Error Responses Error handling Method identified Resume Run Response Yes Yes Error noted in log Error Method execution noted in log Control Method terminates Error handling method executes Application Run resumes starting with the next sample in the Sample List Run Complete notification displays with errors see log for details No Yes Error noted in log Control Method terminates Application Run resumes starting with the next sample in the Sample List Run Complete notification displays with errors see log for details 7 6 TRILUTION LH Software Version 2 0 User s Guide Method Builder Error Handling Error Responses Continued Error handling Method identified Resume Run Response Yes No Error noted in log Run terminates Error handling method executes Error Method execution noted in log Run Complete notification displays with errors see log for details No No Error noted in log Run terminates Run Complete notifi
32. ID Syringe Name Size ul Reservoir Name gt A A 1000 Reservoir B B 1000 Reservoir The 402 Pump Dual Properties Window Properties 402 Pump Dual Properties Boas Default Property Name Brief Description Value Range Setup Syringes to Use The syringes that will be used when this A B A B instrument is selected as the Pump ina Task ID A unique identifier to address syringes on A B A B the instrument 402 Pump Dual Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 21 uoleinbyuo e Nurze Creating a Configuration Method Builder Configuration 402 Pump Dual Properties Continued Default Property Name Brief Description Value Range Syringe Name A unique name to address syringes on the A B N A instrument Size uL The capacity of each installed syringe Fora 1000 100 250 500 1000 5000 10000 25000 range of suggested flow rates for this syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid Reservoir N A that is flowing through the associated syringe It is important for sample tracking General Instrument Name The name that will be used to identify this Pump N A instrument in a Task GSIOC ID A unique numerical address that must be 0 0 31 known to the instrument and to the software that allows the software to communicate to the instrument 402 Pump Dual Properties Table Pa
33. N OO OW fH W OU N WO UW BRWN gt sks Liquid Handling Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume delivered Move Z to Z Safe Height TSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Dilute This task aspirates the specified volume of liquid source from the tray and another liquid diluent from the reservoir tray or transfer ports and then dispenses the combined volume to the wells of the result zone For more information about the properties on each tab see Dilute Properties on page A 46 Dilute Advanced Rinsing on page A 49 Dilute Instruments on page A 51 Dilute Sequence of Steps on page A 52 TRILUTION LH Software Version 2 0 User s Guide anid Dilute Tasks Liquid Handling Dilute Properties A Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Sourc
34. TRILUTION LH Software Version 2 0 User s Guide K 23 AINN DOISD GSIOC Utility GSIOC Utility K 24 Send an Immediate Command Basic Mode 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to 2 Type or select a Command 3 Click Cimes Advanced Mode Using the button 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to 2 Type a Command and Comment The comment is optional 3 Click Cms Using Execute from the Edit menu 1 Highlight the command to be sent in the command list 2 Choose Selection from the Edit menu and select Execute Or type Ctrl E Using the command list Double click on the command line of the command you want to send TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility Send a Buffered Command Basic Mode 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to 2 Type or select a Command 3 Click mea Advanced Mode Using the button 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to 2 Type a Command and Comment The comment is optional 3 Click sured Using Execute from the Edit menu 1 Highlight the command to be sent in the command list 2 Choose Selection from the Edit menu and select Execute Or type Ctrl E Using the command list Double click on the command li
35. Z Arm Type mm This is the height of the Z arm installed on the 123 mm Z Arm 56 mm Z Arm liquid handler 123 mm Z Arm 183 mm ZArm Z Safe Height mm The Z height to which the instrument s probe 215 Calculated based on moves before moving in the X or Y direction 223 Sample Changer Properties Table Page 1 of 2 Z Clamp Height and Z Arm Type Range provided on screen TRILUTION LH Software Version 2 0 User s Guide 4 19 uoleinbyuos e Guljeai gt Creating a Configuration Method Builder Configuration 223 Sample Changer Properties Continued Property Name Brief Description Default Value Range Contacts 24V Output Optionally type a description for the contact This N A N A Output 1 description will be used to identify the contactina Output 2 Task Output 3 Output 4 Input A Optionally type a description for the contact This N A N A Input B description will be used to identify the contactina Input C Task Input D General Instrument Name The name that will be used to identify this instrument in a Task Liquid Handler N A GSIOC ID A unique numerical address that must be known to the instrument and to the software that allows the software to communicate with the instrument 223 Sample Changer Properties Table Page 2 of 2 10 10 19 4 20 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 402 Pump Dual
36. e Click OK TRILUTION LH Software Version 2 0 User s Guide Task Builder Importing Tasks 1 Inthe Task Builder click IMPORT The Select Task Exported Files window displays Select Task Exported Files Look in E Working J e 0g m Ti Add then T Ory El Agutte Z Eject Tips hte mi Aspeate weh Tips hta T Ekee hte El Aspeate tte Zi Evaporate te a Cherry Pick wath Tips he BDiractionste thte TI Cherry Pek hte T GSTOC hte Ef Condhion the Zl inject hte Ti Delver wth Tips tte SBI Ar Push hte T Delver hte T IT Aspirate bte Zi Derivatize he EIT Eject Tipse Ei vbse he GAN Laud Level Detection hte T Dinense fandom ta Random with Tips hto LIT Load Tips the Ei Deperne Random to Rendon Pte an Mix he E Depense wth Tios hte SII Rinse Probes Alkguothte T Dipensn hta T IT Rinse Probes hte lt File name Upen Fies ol type Task Exported Files kto Cancel The Select Task Exported Files window 2 From the Select Task Exported Files window complete the following a Browse for and select the Task Exported file s To select multiple Tasks in a sequential order hold SHIFT any arrow key simultaneously To select Tasks in a random order hold CTRL and select multiple Tasks with the mouse b Click OPEN On completion of the Import operation the TRILUTION information box displays TRILUTION LH Software Version 2 0 User s Guide sse bulzaoduy Importing Tasks Task Builder
37. for a selected operator or expression Toolbar Provides quick access to some options available in the Method window To activate a tool click on its icon Undo Redo The Undo J and Redo buttons provide the ability to Undo and Redo the last action within the Method TRILUTION LH Software Version 2 0 User s Guide Method Builder Method Variable List Lists the available variables for the Method Click the button to view the list of variables used in the Method Click NEW VARIABLE to add additional variables or highlight an existing variable and click MODIFY Back The Back button M provides the ability to return focus to the left panel in the workspace Right click menu Task Right click on a Task icon to display the following menu options Menu Description Cuts the Task for deleting or pasting in a new location in CUT the workspace in an operator or expression or in another Method Copies the Task to duplicate in a new location in the COPY workspace in an operator or expression or in another Method TRILUTION LH Software Version 2 0 User s Guide 6 5 MOPUIM Gel pour Method Tab Window Method Builder Method 6 6 Menu Description Places a cut or copied Task in the workspace in an operator PASTE l or expression or in another Method DELETE Removes the Task from the workspace LINE BREAK A toggle for moving Tasks after the selected Task to the
38. next line below the selected Task PROPERTIES Opens the task property page for the selected Task OPEN TASK Opens the Task in the Task Builder Workspace Right click in the workspace to display the following menu options Menu Description Places a cut or copied Task in the workspace in an PASTE operator or expression or in another Method VIEW EXTENDED Hides the right panel in the workspace VIEW NORMAL Shows the right panel in the workspace TRILUTION LH Software Version 2 0 User s Guide Method Builder Method 6 Creating a Method Create a Method by adding new Tasks Variables Operators and Expressions to the workspace The drag and drop feature allows easy creation of a Method You can drag and drop Tasks Variables Expressions and Operators into the workspace Saved Methods are listed in the Methods palette A Task is positioned anywhere in the Method Builder workspace depending on where the Task is dropped To create a Method Do one or both of the following one or many times e From the Tasks palette drag a Task and then drop it in the workspace The task property page for that Task appears Review the values for each task property and modify if necessary e From the Operators palette drag an expression variable or operator and then drop it in the workspace TRILUTION LH Software Version 2 0 User s Guide 6 7 poy w e burjeal gt Variables Method Builder Meth
39. or Pulse the specified contact VALVEMATE 1 Switch valve to specified position TRILUTION LH Software Version 2 0 User s Guide F 19 UOIUSOd AJLA 39S Wait Tasks Auxiliary F Wait This Task waits a specified length of time or initiates a wait in the Method until a contact state change is detected on the specified input contact For more information about the properties on each tab see Properties Bitestruments i _ _ Wait Properties on page F 20 teow Wait Instruments on page F 21 G gt Time kA Contect Time Wait Sequence of Steps on page F 22 Wee Time min foi Wait Properties Property Name Brief Description Default Value Wait For Select Time or Contact Time Wait Properties Table Page 1 of 2 F 20 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary F Wait Properties Continued Property Name Brief Description Default Value Time Time the program waits before starting the next task 0 01 min Wait Time Contact The input contact being monitored for a state change Valid range is A E A Contact Name Wait Properties Table Page 2 of 2 Wait Instruments Instrument The instrument type that the command will affect Property Name Brief Description Default Value Wait Instruments TRILUTION LH Software Version 2 0 User s Guide F 21 uem Wait Tasks Auxiliary Wait Sequence of Steps
40. rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe No rinse is performed O uL when the Outside Volume is 0 Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Prime Pump with Tips Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide C 63 sdi yyim duing Wld Prime Pump with Tips Tasks Disposable Tips Prime Pump with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip wil
41. s Guide UOd puo gt Condition Tasks SPE INSIDE RINSE mM A U N gt Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate Move Z to top OUTSIDE RINSE U A U N gt Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Height from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Move Z top GAS AIR PURGE SPE 215 ONLY U A U N gt Move Z to top Set Voltage On and Output Contact 1 to Close Wait 0 02 min Set Voltage Off and Output Contact 1 to Open Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Dry This Task dries the DECs by pushing pressurized gas or air from the syringes through the DECs with the mobile rack positioned over the drain For more information about the properties on each tab see Dry Properties on page D 20 Dry Advanced Rinsing on page D 22 Dry Instruments on page D 23 Dry Sequence of Steps on page D 23 TRILUTION LH Software Version 2 0 User s Guide D 19 Aig Dry Tasks SPE D Dry Properties Property Name Brief Description Default Value DEC Zone The zone to which the Reservoir Volume is delivered DEC Well The well
42. the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Result Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone SS Aliquot Advanced Inside Rinse Table Page 1 of 3 2mm TRILUTION LH Software Version 2 0 User s Guide B 23 yonbily SS SS Aliquot Tasks SS Liquid Handling SS Aliquot Advanced Inside Rinse Continued Property Name Brief Description Default Value Inside Rinse Desired position of the solvent selection valve on the GX Solvent Sys
43. 0 User s Guide D 27 Kaq Elute Tasks SPE D Elute This task aspirates the specified volume of liquid source from the reservoir tray or transfer ports and then dispenses the volume to the wells of the DEC zone with the mobile rack over the collect position Properties Advanced ttining flimstruments SS For more information about the properties on each tab see Flute oo Elute Properties on page D 29 Elute Advanced Rinsing on page D 33 Elute Instruments on page D 35 Elute Sequence of Steps on page D 36 D 28 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Elute Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into the probe For a range of 3 mL min Source Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts a
44. 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm Click MOVE Z value Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the the circuit is open off boxes 24V Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the the circuit is open off boxes Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the circuit is open GX 271 Liquid Handler Manual Control Table Page 2 of 4 TRILUTION LH Software Version 2 0 User s Guide 9 47 MOpUIM qe J01 Uu0 enuey Manual Control Tab Window Application Manual Control 9 GX 271 Liquid Handler Manual Control Continued Property Name Brief Description Default Value Use Toggle the solenoids off on by selecting and clearing the boxes Solenoids 1 A filled box indicates that the solenoid is 2 on An empty box indicates that the solenoid is off Pump Valve Position Select the solvent selection valve 2 position to use when priming Click PRIME Prime Flow Rate The speed at which the solvent moves 5 mL min out of the probe Injection Valve Indicate whether a GX Direct Inje
45. 1 A filled box indicates that the solenoid is 2 on An empty box indicates that the solenoid is off Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position GX 274 Liquid Handler without Pump Manual Control Table Page 3 of 3 Toggle the solenoids off on by selecting and clearing the boxes 9 58 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control GX 281 Liquid Handler The GX 281 Liquid Handler Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons GX 281 Liquid Handler Manual Control Property Name Brief Description Default Value Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number 2 mm is entered or down when a negative number is entered from the Tube Bottom GX 281 Liquid Handler Manual Control Table Page 1 of 4 Use Click MOVE TO WELL TRILUTION LH Software Version 2 0 User s Guide 9 59 MOPpUIM qe O4 U0D jenuew Manual Control Tab Window Application Manual Control 9 GX 281 Liquid Handler M
46. 13 Dispense Air Gap at Air Gap Flow Rate TRILUTION LH Software Version 2 0 User s Guide B 123 XIW SS SS Mix Tasks SS Liquid Handling MIX WITH AIR CON WD ON A U N gt 9 Move Z to Z Safe Height Aspirate Air Gap at Flow Rate Move probe to Mix Well in Mix Zone Move Z to Z Safe Height Aspirate Mix Volume at Flow Rate Lower probe into well to Result Height Dispense Mix Volume at Flow Rate Solvent Valve at Solvent Valve Position selected Wait Equilibration Time Repeat 4 8 until Number of Mixes completes 10 Perform a Touch Off X direction if selected and move Z to Z Safe Height 11 Move probe to Inside Rinse Well in Inside Rinse Zone 12 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 13 Dispense Air Gap at Flow Rate INSIDE RINSE 1 2 3 4 B 124 Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Switch solvent valve to Inside Rinse Solvent Valve Position TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B 5 Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse
47. 2 0 User s Guide 4 79 uoleinbyuos e Gules Creating a Configuration Method Builder Configuration Quad Z 215 Properties Continued Property Name Brief Description Default Value Range Input A Optionally type a description for the contact N A N A Input B This description will be used to identify the Input C contact in a Task Input D General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task GSIOC ID A unique numerical address that must be 22 20 29 known to the instrument and to the software that allows the software to communicate with the instrument Quad Z 215 Properties Table Page 3 of 3 4 80 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration SPE 215 Size pL A Properties Property Name Setup SPE 215 1000 1000 1000 1000 1000 1000 1000 1000 The SPE 215 Properties Window Reservoir Name Reservoir Reservoir Reservoir Reservoir Reservoir Reservoir Reservoir Reservoir SPE 215 Properties Brief Description Default Value Range Z Clamp Height mm This number corresponds to where the mounting clamp is on the vertical ruler on the installed Z arm SPE 215 Properties Table Page 1 of 3 178 0 178 Range provided on screen TRILUTION LH Software Version 2 0 User s Guide 4 81 uoleinbyuos e Nurze Creating a Configuration Metho
48. 2 0 User s Guide Method Builder Configuration GX 274 ASPEC without Pump The GX 274 ASPEC without Pump Properties Window Properties GX 274 ASPEC without Pump Properties Property Name Brief Description Default Value Range Setup Probe Type mm If the stop pin was inserted in S1 select 56 mm 125 mm Probe 56 mm Probe Probe If the stop pin was inserted in S2 select 125 mm Probe If no stop pin was used select 175 mm Probe GX 274 ASPEC without Pump Properties Table Page 1 of 3 125 mm Probe 175 mm Probe TRILUTION LH Software Version 2 0 User s Guide 4 59 uoljeinbyuo gt e Gules Creating a Configuration Method Builder Configuration GX 274 ASPEC without Pump Properties Continued Property Name Brief Description Default Value Range Z Clamp Height mm This number corresponds to where the mounting 185 Calculated based clamp is on the vertical ruler on the installed on Z Clamp Height Z arm and Probe Type Range provided on screen Z Safe Height mm The Z height at which the instrument s probe 185 Calculated based moves to before moving in the X or Y direction on Z Clamp Height and Probe Type Range provided on screen Contacts Output 1 Optionally type a description for the contact This N A N A Output 2 description will be used to identify the contactina 24V Output 1 Task eo ues Additionally indicate whether the contact should open close or remain in the
49. 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and intothe 10 mL min outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 SS Dilute Outside Rinse Table Page 1 of 4 B 68 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Dilute Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from
50. 4 B 54 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Derivatize Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Derivatize Outside Rinse Table Page 4 of 4 SS Derivatize Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Derivatize Instruments B 55 TRILUTION LH Software Version 2 0 User s Guide 9ZILALIAG SS SS Derivatize Tasks SS Liquid Handling B SS Derivatize Sequence of Steps The following is an overview for the sequence of steps Aspirate on page B 56 Dispense on page B 57 Mix on page B 58 Inside Rinse on page B 59 Oa BP WN Outside Rinse on page B 59 ASPIRATE Reservoir Move Z to Z Safe Height Move probe to Solution Well in Solution Zone Aspirate Solution
51. 4 93 Rate Transfer Port The speed at which the Diluent Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source and Diluent Volumes are delivered Result Well The well in the Result Zone to which the Source and Diluent Volumes are 1 delivered When using a multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source and Diluent Volumes move out of the probe Fora 5 mL min range of suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Dilute Properties Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Liquid Level Following Dilute Advanced Rinsing Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height Diluent Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option A Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liqui
52. 4 of 4 SS Dispense Random to Random Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Dispense Random to Random Instruments TRILUTION LH Software Version 2 0 User s Guide B 97 wopuey 0 wopuey su ds q SS SS Dispense Random to Random Tasks SS Liquid Handling B SS Dispense Random to Random Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 98 2 Dispense on page B 98 3 Inside Rinse on page B 99 4 Outside Rinse on page B 99 ASPIRATE Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Nun A U N gt DISPENSE Move probe to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Nn BWN B 98 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B 7 If Source Volume is great
53. 69 uoleinbyuo e Nurze Creating a Configuration Method Builder Configuration Property Name Adjust Minipuls 3 Properties Continued Brief Description A percentage value to adjust flow rate for the Minipuls Flow rate cannot exceed the maximum allowed for the tubing selected Default Value 0 Range 100 100 Transfer Tubing mL Other Tubing mL Tubing that is being used on the Minipuls in this Configuration When selected the non standard size of the tubing being used on the Minipuls 5 0 0 2 0 4 1 0 2 8 3 0 5 0 10 0 0 99 Pump Head Type The pump head installed on the Minipuls Standard Standard High Flow Number of Channels The number of channels that will be used when this instrument is selected as the Pump in a Task 1 2 4 8 Channels The channels that will be used when this instrument is selected as the Pump in a Task A B C D E F G H ID A unique identifier to address the channel on the instrument A B C D E F G H Channel Name A unique name to address the channel on the instrument Minipuls 3 Properties Table Page 2 of 3 N A 4 70 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Minipuls 3 Properties Continued Property Name Brief Description Default Value Range Tubing ID mm The capacity of the tubing for each None See Tubing Options an
54. 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select the Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 50 uL Source Air Gap a range of suggested flow rate values see Flow Rates on page 4 93 Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 6 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Wash Properties Table Page 2 of 5 TRILUTION LH Software Version 2 0 User s Guide D 103 gt V gt Wash Tasks SPE D Wash Properties Continued Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate DEC Zone The zone to which the Reservoir Volume is delivered DEC Well The well in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a va
55. 93 Result Zone The zone to which the Source Volume is delivered Starting Well The well in the Result Zone to which to start dispensing the source volume Enter 1 x where x is the name defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 SS Cherry Pick Properties Table Page 1 of 2 B 34 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Cherry Pick Properties Continued Property Name Brief Description Default Value Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction SS Cherry Pick Properties Table Page 2 of 2 SS Cherry Pick Advanced Inside Rinse Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height and Result Heigh
56. A O 0000o o0 o0 0o SOUES OE 4 Rinse Station XL Left TRILUTION LH Software Version 2 0 User s Guide G 9 suo1 e gt 07 asuly Rinse Locations Rinse Locations Rinse Station XL Short Left Rack TRILUTION Label Inside Rinse Drain XL 0mm rinse depth Left Well Outside Rinse XL Shallow 10 mm rinse depth Shallow Rinse Center Well Outside Deep Rinse Location Right Well Outside Deep Rinse Right Well Rinse XL Flow 45 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Station XL Tall Left Rack TRILUTION Label Inside Rinse Drain XL Left Well 0 mm rinse depth Outside Shallow Rinse Location Center Well Outside Rinse XL Shallow Shallow Rinse Center Well 10 mm rinse depth _ Outside Deep Rinse Location Right Well Outside Deep Rinse XL Deep Rinse Right Well 85 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide suol e907 asuly Rinse Locations Rinse Locations SOLES 0F Rinse Station XL Right Footprint Rinse Station XL Right k Rinse Station XL Short Right Rack TRILUTION Label Inside Rinse Drain XL 0 mm rinse depth Right Wells Outside Rinse XL Shallow 10 mm rinse depth Shallow Rinse Center Well Outside Deep Rinse Locations Left Wells Ou
57. Air Gap at Air Gap Flow Rate Lower probe into well to Solution Height Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height On A WN gt Tray 1 Move Z to Z Safe Height 2 Move probe to Source Well in Source Zone 3 Aspirate Source Air Gap at Air Gap Flow Rate 4 Lower probe into well to Source Height B 56 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Move probe to Solution Well in Solution Zone oOo N O WM Aspirate Solution Air Gap at Air Gap Flow Rate 9 Lower probe into well to Solution Height 10 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 11 Move Z to Z Safe Height DISPENSE Move probe to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position BR WN gt Dispense Source Volume Solution Air Gap Solution Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Dispense Extra Volume Source Air Gap at Result Flow Rate 0 Move Z to Z Safe Height O ON A WI TRILU
58. Brief Description Default Value Source Select Reservoir or Tray Reservoir Reservoir Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Reservoir The quantity of a sample O uL Source Volume Tray The zone from which the Source Volume is drawn Source Zone Tray Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray The well in the Source Zone from which the Source Volume is drawn 1 Source Well Tray The quantity of a sample O uL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume SS Derivatize Properties Table Page 1 of 3 B 46 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Derivatize Properties Continued Property Name Brief Description Default Value Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray
59. Calculated based on before moving in the X or Y direction Z Clamp Height and Z Arm Type Range provided on screen 222 XL Liquid Handler Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 17 uoleinbyuos e Nurze Method Builder Configuration 4 222 XL Liquid Handler Properties Continued Property Name Brief Description Default Value Range Creating a Configuration Contacts Relay Output 1 Optionally type a description for the contact This N A N A Relay Output 2 description will be used to identify the contact in a Relay Output 3 Task Output 4 Output 5 Output 6 Output 7 Output 8 Analog Input Optionally type a description for the contact This N A N A Input 1 description will be used to identify the contact in a Input 2 Task Input 3 Input 4 General Instrument Name The name that will be used to identify this instrument Liquid Handler N A in a Task GSIOC ID A unique numerical address that must be known to 10 0 31 the instrument and to the software that allows the software to communicate with the instrument 222 XL Liquid Handler Properties Table Page 2 of 2 4 18 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 223 Sample Changer The 223 Sample Changer Properties Window Properties 223 Sample Changer Properties Property Name Brief Description Default Value Range Setup
60. Control 9 GX 271 Liquid Handler without Pump Manual Control Continued Property Name Brief Description Default Value Use Solenoids 1 2 A filled box indicates that the solenoid is on An empty box indicates that the solenoid is off Toggle the solenoids off on by selecting and clearing the boxes Injection Valve Indicate whether a GX Direct Inject Valve is installed Valve Position Rotates the valve to the selected Click SWITCH position Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position GX 271 Liquid Handler without Pump Manual Control Table Page 3 of 3 9 52 TRILUTION LH Software Version 2 0 User s Guide Applicatio GX 274 ASPEC n Manual Control without Pump laterna 19 0 The GX 274 ASPEC without Pump Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons GX 274 ASPEC without Pump Manual Control Froperty Brief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone to 1 which probe A will move Z Offset The distance up when a positive number 2 mm C
61. Control Table Page 1 of 3 9 56 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 GX 274 Liquid Handler without Pump Manual Control Continued ore Brief Description Default Value Use Name X Enter anumber to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter anumber to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0mm l Click MOVE Z value Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off on 2 closed on An empty box indicates that by selecting and clearing the boxes the circuit is open off 24V Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off on 2 closed on An empty box indicates that by selecting and clearing the boxes the circuit is open off Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the circuit is open GX 274 Liquid Handler without Pump Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 57 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control GX 274 Liquid Handler without Pump Manual Control Continued Property Brief Description Default Value Use Name Solenoids
62. Created By Atina Vanables Name Te Vahva Show Le P arest Aute lacremess Eper Tew Te low me FR pad Herdar romred Ugs Herder THe ras Probe Equblratien Tre TOE tom TE FASE Probe Source TEFAF Teue rat Denke Cuma Flow Rate FLOW RATE Soin Te Fase Probe Vokri vuM ea mwa FAX Bare psa Dro THE FAs Steps Command Aspire Inetrimert Mane Rep ISTINE Synchronize wa lt ON Lt insna Liquid Handler DISTRUNDT Syringe Pick Syringe SYSTIR Valve Position e PROGE LE VALVE Zanple Volse Probe Voiuse ul VOLIE Emra Volume oul vac Flow mute Probe Source Flow fate sL ain PLOW RATE Use LU False THUE ALSE Command Wait Smchronise n d aor Wait Tise Prote Equilibration Time sin TINE gt Alarm False THUE PALZE 3 Label Operator Declare Label As End of Task TRE tem TRRUTION LH Software Popii TRILUTION LH Software Version 2 0 User s Guide MopuIM syod y yL The Reports Window Generating Reports 11 6 11 Application Report This report comprises two parts a brief summation of the application information application name and version created by created date and description and sample list details including values for variables Run Report This report comprises three parts a brief summation of the application run information application name application version run name run date run by and run mode sample list details including values for variables captu
63. Creating a New Variable 1 From the Operators palette drag and drop the Variable operator into the Workspace The Variable List window displays 2 Inthe Variable List window click NEW VARIABLE The Variable dialog appears 3 Inthe Variable dialog do the following a In the Variable Name field type a unique Variable name b Select a Variable Type from the drop down provided c Select or type the default value for the Variable Type d Select Show In Parent to display the Variable in the Method Builder if creating a Task variable or in the Sample List if creating a Method variable e Select Auto Increment in Sample List to advance one well in each iteration that uses the variable This option only functions for Variable Type Well f Optionally add values to the Value List by typing the value and then clicking ADD Values entered in this list display as drop down values in the Task or Method in which the variable is used 4 Doone of the following e Click NEW to add additional variables e Click OK to exit the Variable window TRILUTION LH Software Version 2 0 User s Guide 10 10 3 sajqeie Variables Working with Variables 10 4 10 Viewing and or modifying variable properties 1 Click the Variable List icon E 2 Select a variable from the variable list and do one of the following Double click the variable Right click on the variable and choose MopIFY from the submenu Click MODIFY
64. D 90 Load and Collect Sequence of Steps on page D 91 TRILUTION LH Software Version 2 0 User s Guide D 83 29 0 gt pue peo Load and Collect Tasks SPE D Load and Collect Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into and out of the probe For a 10 mL min Source Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 25 uL Source Air Gap Load and Collect Properties Table Page 1 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load and Collect Properties Continued Property Name Brief Description Default Value Tray The speed
65. Dialog whereas Archive removes it Both allow the data to be restored 2 Select a folder and then click SAVE On completion of the Archive operation the Run is saved in the specified folder with a LHRE extension The TRILUTION information dialog box is displayed 3 On the TRILUTION information dialog box do the following e To view the log information of the Archive operation click DETAILS e Click OK TRILUTION LH Software Version 2 0 User s Guide Application Restoring a Run You can restore a run that has been exported or archived To restore a run 1 Onthe Run Results window click RESTORE 2 Browse for and select the Run Archived Files To select multiple runs in a sequential order hold SHIFT any arrow key simultaneously To select runs in a random order hold CTRL and select multiple runs with the mouse 3 Click OPEN On completion of the Import operation the TRILUTION information box is displayed If a Task or any Task placed within a Task with the same name is found an option is provided to import the Task as a new version import all Tasks as new version skip the import of all Tasks or rename the Task 4 On the TRILUTION information dialog box do the following To view the log information of the Import operation click DETAILS e Click OK TRILUTION LH Software Version 2 0 User s Guide 8 25 MopulM s Nsay UNy Application Manual Control 9 In addition to timed runs TRILUTION
66. Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source Volume is delivered 1 Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction SS Transfer Properties Table Page 2 of 2 B 147 TRILUTION LH Software Version 2 0 User s Guide JBJSUPAL SS SS Transfer Tasks SS Liquid Handling Property Name Liquid Level Following SS Transfer Advanced Inside Rinse Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid
67. F Auxiliary Auxiliary Tasks Gilson Task Name and Description GSIOC This task sends a GSIOC command to the specified instrument or unit ID Prompt This task displays a prompt dialog with the selected message Run Executable This task invokes an external application MMM i Scan Bar Codes This task scans the racks on the locator plate of the GX 281 Liquid Handler for the purpose of ensuring that the rack hardware setup matches the software rack Bed Layout setup Auxiliary Tasks Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide F 3 Tasks Auxiliary F Auxiliary Tasks Continued Set Electrical Contact This task opens and powers off closes and powers on or pulses the specified output contact on the specified instrument Set Valve Position This task opens and powers off closes and powers on or pulses the specified contact solenoid on the specified instrument or changes the valve position on the VALVEMATE Wait This Task waits a specified length of time or initiates a wait in the Method until a contact state change is detected on the specified input contact Auxiliary Tasks Table Page 2 of 2 Se F 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary GSIOC This task sends a GSIOC command to the specified instrument or unit ID For more information about the properties on each tab see s GSIOC
68. Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate 5 Dispense Inside Volume at Inside Rinse Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N WO UW A WN TRILUTION LH Software Version 2 0 User s Guide A 31 Id Kaay Derivatize Tasks Liquid Handling A Derivatize This task performs an add aspirates the specified volume of liquid source from the reservoir tray or transfer ports and another liquid solution from the tray and then dispenses the combined volume to the wells of the result zone and then mixes with liquid or air Properties Advanced niosing Bastrments S FOr more information about the properties on each tab see Derivatize oo Derivatize Properties on page A 33 Derivatize Advanced Rinsing on page A 37 Derivatize Instruments on page A 39 Derivatize Sequence of Steps on page A 40 A 32 TRILUTION LH Software Version 2 0 User s Guide Tasks Liqui
69. Guide C 57 sdi L yum XIW Mix with Tips Tasks Disposable Tips 9 If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 10 Move probe to well in Inside Rinse Zone 11 Lower probe into well to Inside Rinse Height 12 Dispense Air Gap at Flow Rate 13 Move Z to top EJECT TIPS OPTIONAL If Eject Tips After Task TRUE 1 Move Z to top 2 Move probe to Tip Waste Zone 3 Eject tips by moving Z to Z Clamp Height INSIDE RINSE OPTIONAL 1 Move Z to top 2 Move probe to well in Inside Rinse Zone C 58 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips 3 4 5 6 Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Flow Rate Dispense Inside Volume at Inside Flow Rate Repeat steps 4 5 until Inside Volume delivered OUTSIDE RINSE OPTIONAL Nn A U N gt Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate Repeat steps 4 5 until Outside Volume delivered RINSE TOUCH OFF Side Move Z to top Move probe to well in Inside Rinse Zone Move probe to Side Rinse Z Offset Move probe 1 5 mm in the Y
70. In the USERS tab select a User and click DELETE A delete confirmation dialog box displays 2 Click YES The selected User is deleted To view Group Permissions The Users and Groups tabs both provide the ability to view Group permissions View Group Permissions using the Users tab 1 Onthe Users and Groups window select a User from the Users tab The assigned Group name s and description s for the User display in the right frame 2 Click MODIFY The Modify User window displays TRILUTION LH Software Version 2 0 User s Guide 1 31 snual H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 3 Inthe Select the Group section click VIEW PERMISSIONS a When multiple Groups are selected TRILUTION LH displays a combination of all Group permissions b When one Group is selected TRILUTION LH displays permissions for that specific Group 4 On the View Group Access Permissions window view the following a In the Group Name field the Group Name b lt In the Group Description field optional a brief description of the Group c In the Permissions table the permissions assigned to the group for the builders d Click OK Click CANCEL in the Modify User window to avoid saving Group changes to the User Log Off This option ends the current user s session and allows a different user to log on to TRILUTION LH 1 32 TRILUTION LH Software Version 2 0 User s Guide TRILUTION
71. JaysuelL Transfer with Tips Tasks Disposable Tips Transfer with Tips Tips Continued Property Name Brief Description Default Value Eject Tips After Select and all tips will be ejected before the rinses Active Task Clear and the tips will remain loaded Prewet Tip Select this option to wet the tip before aspirating the Source Volume 1st Iteration Cleared only Prewet Volume The quantity of liquid source aspirated and dispensed to prewet the tip when 10 uL Prewet Tip has been selected Touch Off Mode Used when Touch Off was selected on the Properties tab select Side or Surface Surface touch off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Surface number is entered from an auto calculated position to which the tip will move Z Offset when performing a surface Touch Off Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Touch Off The distance right when a positive number is entered or left when a negative 0 mm Mode Side number is entered from the edge of the tube that the tip will move when X Offset performing a side Touch Off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch O
72. LH also provides manual control over system components Manual control is useful for preparing a system for a run Note Before running your system in manual mode be sure that the instruments in the system are turned on and the appropriate connections are made as described in each instrument s user s guide Manual control is accessed from the Application window TRILUTION LH Software Version 2 0 User s Guide 9 1 Manual Control Tab Window Application Manual Control 9 2 Manual Control Tab Window The Manual Control window within the Application is a graphical user interface used to control the instruments within the configuration Manual Control Properties by Instrument Every instrument has a set of manual moves that differ for each instrument Instrument Name For List of Properties 215 Liquid Handler See page 9 4 215 Liquid Handler without Pump See page 9 7 215 SW Liquid Handler See page 9 9 221 XL Liquid Handler See page 9 11 222 XL Liquid Handler See page 9 14 223 Sample Changer See page 9 17 402 Pump Dual See page 9 19 402 Pump Dual with Tee See page 9 21 402 Pump Single See page 9 23 406 Pump Dual See page 9 25 406 Pump Single See page 9 27 444 QuadDilutor See page 9 28 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control Instrument Name 506C System Interface For List of Proper
73. Liquid Handling Property Name Outside Rinse Zone SS Dispense Random to Random Advanced Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Dispense Random to Random Advanced Outside Rinse Table Page 1 of 4 B 94 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Dispense Random to Random Advanced Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom
74. Menus TRILUTION LH Software Overview 1 A Group with associated Users cannot be deleted until the Users are removed from the Group The Admin group cannot be deleted View Group Permissions using the Groups tab 1 Onthe Users and Groups window select a Group from the Groups tab Click MODIFY The Modify Group window displays On the Modify Group window view the following a In the Group Name field the Group Name b In the Group Description field optional a brief description of the Group c In the Permissions table the rights assigned to the group for the builders 4 Click CANCEL to avoid saving any unwanted changes 1 26 TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Users Users and Groups The Users tab Users are assigned to Groups based on their defined roles For Example e Operator e Administrator e Scientist The Users tab lists all the available Users You can create modify and delete Users Users can be assigned to one or more available groups TRILUTION LH Software Version 2 0 User s Guide snuaW H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview To create a new User 1 1 28 On the Users and Groups window click USERS tab The default Users Administrator and Analyst display with their Group name in the right window Click CREATE The Create User window appears Create User The Cr
75. Method This section takes you through the procedures for adding steps to a Method TRILUTION LH Software Version 2 0 User s Guide Method Tab Window Method Builder Method 6 Method Tab Window The Method Tab window is a graphical interface used to set the steps ina Method Dj Method Suiider Untithed Mathods J Vaude Expresson If rxilf The Method Builder Method Tab Window 6 2 TRILUTION LH Software Version 2 0 User s Guide Method Builder Method It has the following sections Section Described on Methods Palette page 6 3 Operators Palette page 6 3 Tasks Palette page 6 4 Workspace page 6 4 Methods Palette Lists the Methods created Operators Palette Lists the operators variables and expressions TRILUTION LH Software Version 2 0 User s Guide 6 3 MOPUIM Gel pour Method Tab Window Method Builder Method 6 4 Tasks Palette Lists the available Tasks by function For a detailed description of the Tasks task properties and a sequence of steps see any or all of the following appendices Appendix A Tasks Liquid Handling Appendix B Tasks SS Liquid Handling Appendix C Tasks Disposable Tips Appendix D Tasks SPE Appendix E Tasks Tweaks Appendix F Tasks Auxiliary Workspace Lists the Operators Variables Expressions and Tasks used in the Method The left panel provides the sequence The right panel contains the details
76. Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top 7 Move probe to well in Inside Rinse Zone 8 Lower probe to Inside Rinse Height from Tube Bottom 9 Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top D 112 If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume not aspirated and TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D AIR PUSH Solenoid 1 Move probe to well in Inside Rinse Zone 2 Aspirate 250 uL at 5 mL min 3 Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Oo ON WD MN RA Syringe 1 Move probe to DEC Well in DEC Zone 2 Move Z to top TRILUTION LH Software Version 2 0 User s Guide D 113
77. Properties on page F 6 GSIOC Sequence of Steps on page F 7 TRILUTION LH Software Version 2 0 User s Guide F 5 DOISD GSIOC Tasks Auxiliary GSIOC Properties Property Name Brief Description Default Value GSIOC Mode The GSIOC Modes GSIOC with ID or GSIOC GSIOC GSIOC with ID command sends a GSIOC command to an instrument at a specified unit ID GSIOC sends a GSIOC command to a specific instrument GSIOC with ID The unit ID of the instrument that the command will affect 1 Unit ID GSIOC The instrument that the command will affect Instrument Name Command Type Select Buffered or Immediate command type Immediate Command The string that sends an instruction to the Instrument String GSIOC Properties F 6 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary GSIOC Sequence of Steps 1 Send Command of Command Type Immediate or Buffered to the Instrument TRILUTION LH Software Version 2 0 User s Guide F 7 DOISD Prompt Tasks Auxiliary F Prompt This task displays a prompt dialog with the selected message For more information about the properties on each tab see i Properties _ _ Prompt Properties on page F 8 Prompt Sequence of Steps on page F 9 Promot l Promotforinout Message Prompt Properties Property Name Description Default Value Prompt Prompt Prompt displays message Prompt for Input dis
78. RESULTS IMPORT EXPORT EMERGENCY STOP NEW OPEN SAVE This option begins the Application run This option causes the run to pause task execution To continue the task execution click RESUME The run will continue as programmed This option begins a graphical representation of the run without moving the instruments This option opens the Run Results window From this window results can be viewed exported archived restored or refreshed Imports a LHAE file and lists the Application in the Applications palette Exports the Application to a specified location as a LHAE file Stop the run Create a new Application Open an existing Application Save an existing Application to the same name TRILUTION LH Software Version 2 0 User s Guide Application Action Button Description SAVE AS Save anew Application or save an existing Application to anew name Optionally type a description DELETE Deletes the open Application or all versions of the Application Info Window Lists run time events and the time they occurred TRILUTION LH Software Version 2 0 User s Guide MOpul UO ed1 ddy Setting Up a Sample List Application 8 Setting Up a Sample List The Sample List is an editable grid that specifies how many samples will be run information about those samples and qualifies any variables in the associated Methods Before you set up a Sample List create the Methods To increment the
79. Rate 5 Dispense Inside Volume at Inside Rinse Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OW A U N gt A 106 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Transfer This task aspirates the specified volume of liquid source from the reservoir tray or transfer ports and then dispenses the volume to the wells of the result zone Properties Advanced ttimsing fPimtrwwents FOr more information about the properties on each tab see c a Transfer Properties on page A 108 Transfer Advanced Rinsing on page A 111 Transfer Instruments on page A 113 Transfer Sequence of Steps on page A 113 TRILUTION LH Software Version 2 0 User s Guide A 107 saysuell Transfer Tasks Liquid Handling Transfer Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample O uL Source Volume Reservoir The speed at which the Source Volume moves into the probe For a range of 10 mL min So
80. Refreshes the Sample List after variables are deleted KO Right click Menu Sample List Select a row then right click and the following options are displayed Menu Description ADD ROW Adds the first row to a new list or adds a row to the bottom of the list ADD MULTIPLE ROWS Adds a user specified number of rows to the bottom of the list INSERT ROW Adds a row between two existing rows TRILUTION LH Software Version 2 0 User s Guide Application Menu Description CLEAR ROW Clears a row of all values but keeps the row in the Sample List DELETE ROW Removes a row from the Sample List OPEN METHOD Opens the Method in the selected row in the Method Builder PASTE ROW SPECIAL Pastes a user specified number of copies of the selected row after the selected row Icon Description ib Run step in Skip step Ul Pause step TRILUTION LH Software Version 2 0 User s Guide 8 7 MopuIiMm uonoddy Application Window Application 8 8 Run Name Type a name for the run 39 characters maximum that will be used to identify the run in the Results window and in reports Defaults to the current date and time at the start of the run Bed Layout Select a Bed Layout from the drop down list to filter Methods to choose for the Sample List in the Application Bed Layout View When creating a Sample List you can view the Bed Layout select multiple wells and have TRILUTION LH generate steps in t
81. Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into and out of the probe For a 6 mL min Source Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Wash Properties Table Page 1 of 5 D 102 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Wash Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into and out of the 6 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4
82. Result Zone to which the Source Volume is delivered 1 Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction SS Dispense Properties Table Page 2 of 2 B 78 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Liquid Level Following SS Dispense Advanced Inside Rinse Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level Detection
83. SS Inject Advanced Outside Rinse Table Page 3 of 4 B 108 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Inject Advanced Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Inject Advanced Outside Rinse Table Page 4 of 4 SS Inject Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Injector The injector or injection module that the task will affect SS Inject Instruments B 109 TRILUTION LH Software Version 2 0 User s Guide p ful ss SS Inject Tasks SS Liquid Handling B SS Inject Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 110 2 Dispense on page B 110 3 Inside Rinse on page B 111 4 Outside Rinse on page B 111 ASPIRATE Move Z to
84. Safe Height DISPENSE 1 Move probe to Result Well in Result Zone 2 Lower probe into well to Result Height TRILUTION LH Software Version 2 0 User s Guide B 155 Tasks SS Liquid Handling B 156 Switch solvent valve to Solvent Valve Position Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height If Source from Tray and Source Volume is greater than Transfer Tubing volume repeat Aspirate Air Gap and Extra Volume not aspirated and Dispense until Source Volume is delivered Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 10 Dispense Source Air Gap Extra Volume at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 4 Switch solvent valve to Inside Rinse Solvent Valve Position Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume Move Z to Z Safe Height TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling OUTSIDE RINSE 1 2 Move probe to first well in Outside Rinse Zone Lower pr
85. Software Version 2 0 User s Guide B 141 Saqojd asuly SS SS Rinse Probes Tasks SS Liquid Handling SS Rinse Probes Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Rinse Probes Outside Rinse Table Page 4 of 4 SS Rinse Probes Instruments Default Value Property Name Liquid Handler Brief Description The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Rinse Probes Instruments B 142 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B SS Rinse Probes Sequence of Steps The following is an overview for the sequence of steps 1 Inside Rinse on page B 143 2 Outside Rinse on page B 143 INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to Inside Rinse Well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 4 S
86. Solution Zone from which the Solution Volume is drawn When 1 using a multiple probe instrument type x where x is a value defined in the Sample List Solution The quantity of solution OuL Volume Solution Air Gap The quantity of the air gap aspirated before the Solution Volume 20 uL Solution Flow The speed at which the Solution Volume moves into the probe For a range of 5 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Solution Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source and Solution Volumes are delivered Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered When using a multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source and Solution Volumes are move out of the probe 5 mL min For a range of suggested flow rate values see Flow Rates on page 4 93 Derivatize Properties Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide A 35 aZNeALAG Derivatize Tasks Liquid Handling Derivatize Properties Continued A Property Name Brief Description Default Value Mix with Air Select box to mix with air Mix with Liquid Select box to mix with liquid Number of Number of times the task should repeat the c
87. Source Volume OuL Diluent Volume Result Zone The zone to which the Source and Diluent Volume are delivered Result Well The well in the Result Zone to which the Source and Diluent Volumes are 1 delivered SS Dilute Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide B 63 23n I0 SS SS Dilute Tasks SS Liquid Handling SS Dilute Properties Continued Property Name Brief Description Default Value Result Flow Rate The speed at which the Source and Diluent Volumes move out of the probe Fora 5 mL min range of suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction SS Dilute Properties Table Page 3 of 3 SS Dilute Advanced Inside Rinse Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height Diluent Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height Diluent Heigh
88. Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 7 If Source Volume is greater than tip volume repeat aspirate and dispense until Source Volume is delivered nn A WN Tray if Extra Volume gt 0 1 Move probe to Result Well in Result Zone 2 Lower probe into well to Result Height 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected 4 Wait Equilibration Time TRILUTION LH Software Version 2 0 User s Guide C 41 sdi y m asuadsig Dispense with Tips Tasks Disposable Tips 5 If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 6 If Source Volume is greater than tip volume repeat aspirate Air Gap and Extra Volume are not aspirated and dispense until Source Volume is delivered 7 Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height 9 Dispense Extra Volume Source Air Gap at Result Flow Rate EJECT TIPS OPTIONAL If Eject Tips After Task TRUE 1 Move Z to top 2 Move probe to Tip Waste Zone 3 Eject tips by moving Z to Z Clamp Height C 42 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips INSIDE RINSE OPTIONAL Onn Bp WN gt Move Z to top Move probe to well in Inside Rinse Zone Lower probe
89. Syringe Time the program waits after a syringe air push before moving out of the well 0 1 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Fractionate Properties Table Page 4 of 5 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Fractionate Properties Continued Property Name Brief Description Default Value Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 1 min Equilibration Time Fractionate Properties Table Page 5 of 5 Fractionate Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated This Following selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to the height at which the li
90. System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Dispense Advanced Outside Rinse Table Page 4 of 4 SS Dispense Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Dispense Instruments B 85 TRILUTION LH Software Version 2 0 User s Guide su ds d SS SS Dispense Tasks SS Liquid Handling B SS Dispense Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 86 2 Dispense on page B 86 3 Inside Rinse on page B 87 4 Outside Rinse on page B 87 ASPIRATE Reservoir 1 Move Z to Z Safe Height Tray Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 1 2 3 4 Lower probe into well to Source Height 5 6 Move Z to Z Safe Height DISPENSE 1 Move probe to Result Well in Result Zone 2 Lower probe into well to Result Height B 86 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Solvent Valve at Solvent
91. TRILUTION LH Software Version 2 0 User s Guide E 25 19A11 A SS SS Deliver Tasks Tweaks SS Deliver Sequence of Steps 1 Dispense Result Volume at Result Flow Rate Solvent Valve at Solvent Valve Position selected 2 Wait Equilibration Time E 26 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Refer to the table on the next page for information about which instruments can use each Auxiliary task TRILUTION LH Software Version 2 0 User s Guide F 1 LL Il ALWINSATWA ALVINSATVA SL 4dS SLZ Z pend SLZ aqord BIA INW sindiuiw 4a pueH pinbr L8Z X5 dund noyymM AajpueH pnb HZ7 XD dund NOY D3AdSW PLZ XD duing noy m Ja pueH pinbry LZ7 XD dajpueH pnb L27 X9 dund NoYyWM D3AdSW LZZ XD 11X DAdSV V1X DAdSV 1X D4dSV jnpow uol gt rafuU 6L8 A9 49AUO0 ZEZ SY 0 JOISD 909 aej19 U Waysks 90S J0IN LGPEND vvt X X X X X X X X Instrument ajbuis dund 90t jeng dwnd 907 ajbuis dund Z0v y m jeng dund Z0r jeng dwnd ZO 1 buey ajdwes Ezz gt x lt 4ajpueH pnb 1X ZZZ x lt 4ajpueH pnb 1X LZZ x 4ajpueH pinb MS SLZ x lt dung moy m AajpueH pnb SLZ x lt gt 4ajpueH pinbr SLZ x lt 2 3 g T o G S z Ye 5 Z jg 8 S lt alas 3 I c Pr W BS vi Cia X n a E ysel TRILUTION LH Software Version 2 0 User s Guide F 2 Tasks Auxiliary
92. Table Page 1 of 2 C 82 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Transfer with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Transfer with Tips Advanced Rinsing Table Page 2 of 2 Transfer with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Transfer with Tips Instruments TRILUTION LH Software Version 2 0 User s Guide C 83 sdi YUM JaysuelL Transfer with Tips Ta Tra sks Disposable Tips nsfer with Tips Sequence of Steps The following is an overview for the sequence of steps N OU A U N gt LO 1 2 AS Load Tips Optional on pa
93. The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Load and Collect Advanced Rinsing Table Page 3 of 3 Load and Collect Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump Load and Collect The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Instruments TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Load and Collect Sequence of Steps The following is an overview for the sequence of steps N OW A U N gt Move Mobile Rack on page D 91 Aspirate on page D 91 Dispense on page D 93 Air Push on page D 95 Inside Rinse on page D 97 Outside Rinse on page D 97 Gas Air Purge on page D 97 MOVE MOBILE RACK 1 Move Z to top 2 Move mobile rack DEC Zone over the collect position ASPIRATE Reservoir 1 Move Z to top 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to DEC Well in DEC Zone 4 Lower probe to Result Height from Tube Top 5 Dispense Source Volume from Reservoir at Result Flow Rate 6 Wait Equilibration Time 7 Move Zto top TRILUTION LH Software Version 2 0 User s Guide D 91 33 05 pue peo Load and Collect Tasks SPE D If Source V
94. The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Solution Zone The zone from which the Solution Volume is drawn Solution Well The well in the Solution Zone from which the Solution Volume is drawn 1 Solution The quantity of a solution OuL Volume Solution Air Gap The quantity of the air gap aspirated before the Solution Volume 20 uL Solution Flow The speed at which the Solution Volume moves into the probe For a range of 5 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Solution Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source and Solution Volumes are delivered SS Derivatize Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide B 47 9ZILALIBG SS SS Derivatize Tasks SS Liquid Handling SS Derivatize Properties Continued Property Name Brief Description Default Value Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered Result Flow Rate The speed at which the Source and Solution Volumes move out of the probe For 5 mL min a range of suggested flow rate values see Flow Rates on page 4 93 Mix with Air Select box to mix with air Mix with Liquid Se
95. Time 1 Wait for Wait Time to elapse Contact 1 Wait for specified input contact to change state F 22 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse locations are assigned to zones in the Bed Layout Builder Inside and outside rinse zones are then chosen in the Task This appendix describes the recommended rinse positions for the 215 Family on page G 2 221 XL Liquid Handler on page G 6 222 XL Liquid Handler and ASPEC XL on page G 9 223 Sample Changer on page G 16 ASPEC XL4 on page G 14 GX 271 Liquid Handler and GX 271 ASPEC on page G 21 GX 274 Liquid Handler and GX 274 ASPEC on page G 24 GX 281 Liquid Handler on page G 28 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Locations Bed Layout Trays Templates 215 SW 215 for Code 517 Rack 215 for Five Code 200 Series Racks 215 for Seven XL Racks Multiple Probe 215 Quad Z 215 Quad Z 215 with Tips SPE 215 Rinse 215 Footprint 215 Family G 2 Rinse 215 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Drain 215 Body Rinse 215 Insert for single probe 215 Rinse 215 Deep Short Insert Rinse 215 Deep Tall Insert Rinse 215 Flow Through Short Insert Rinse 215 Flow Through Tall Insert Rinse 215 Shallow Short Insert Rinse 215 Shallow Tall Insert Inside Rinse Location Left Well Outside or Flow Through Rinse Location Center Well TRILUTION LH Software Ver
96. Tips Optional on page C 56 Mix with Liquid on page C 56 Mix with Air on page C 57 Eject Tips Optional on page C 58 Inside Rinse Optional on page C 58 Outside Rinse Optional on page C 59 N OM A U N gt Rinse Touch Off on page C 59 LOAD TIPS OPTIONAL 1 Move Z to top 2 IfLoad Tips before Task TRUE load tips MIX WITH LIQUID Aspirate Air Gap at Air Gap Flow Rate Move to Mix Well in Mix Zone Lower probe into well to Source Height Aspirate Mix Volume at Flow Rate using Liquid Level Following if selected Lower probe into well to Result Height Dispense Mix Volume at Flow Rate using Liquid Level Following if selected N OU BWN Wait Equilibration Time C 56 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips 8 Repeat steps 3 7 until Number of Mixes is complete 9 Touch Off Optional a If Touch Off TRUE 1 Move probe to edge 2 Move probe to center 3 Move Z to top b if Touch Off FALSE 1 Move Z to top 10 Move probe to well in Inside Rinse Zone 11 Lower probe into well to Inside Rinse Height 12 Dispense Air Gap at Air Gap Flow Rate MIX WITH AIR Aspirate Air Gap at Flow Rate Move probe to Mix Well in Mix Zone Move Z to top Aspirate Mix Volume in Flow Rate Lower probe into well to Result Height Dispense Mix Volume in Flow Rate Wait Equilibration Time Repeat steps 3 7 until Number of Mixes completes CON WO A WU N gt TRILUTION LH Software Version 2 0 User s
97. Top that the tip will move when performing a Z Offset side Rinse Touch Off Dispense with Tips Tips Table Page 2 of 3 C 34 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Dispense with Tips Tips Continued Property Name Brief Description Default Value Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Dispense with Tips Tips Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide C 35 sdi y m asusdsig Dispense with Tips Tasks Disposable Tips Dispense with Tips Advanced Rinsing Property
98. User s Guide F 11 jqe n gt x3 uny Scan Bar Codes Tasks Auxiliary F Scan Bar Codes This task scans the racks on the locator plate of the GX 281 Liquid Handler for the purpose of ensuring that the rack hardware setup matches the software rack Bed Layout setup For more information about the properties on each tab see Properties i Scan Bar Codes Properties on page F 12 m a Scan Bar Codes Sequence of Steps on page F 13 Scan Bar Codes Properties Instrument The instrument that the command will affect Property Name Brief Description Default Value Scan Bar Codes Properties F 12 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Scan Bar Codes Sequence of Steps 1 Scan bar codes of all racks defined in the bed layout TRILUTION LH Software Version 2 0 User s Guide F 13 sapo gt jeg UBS Set Electrical Contact Tasks Auxiliary F Set Electrical Contact This task opens and powers off closes and powers on or pulses the specified output contact on the specified instrument For more information about the properties on each tab see Set Electrical Contact Properties on page F 15 Set Electrical Contact Instruments on page F 15 Set Electrical Contact Sequence of Steps on page F 16 F 14 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Set Electrical Contact Properties Propert
99. Value ASPEC XL4 The transfer port on the ASPEC XL4 to prime A Transfer Port ASPEC XL4 The zone from which the Volume is drawn Zone Prime Transfer Ports Properties Table Page 1 of 3 A 98 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Prime Transfer Ports Properties Continued A Property Name Brief Description Default Value ASPEC XL4 The quantity of liquid aspirated for the prime OuL Volume ASPEC XL4 The speed at which the Volume moves into the probe For a range of suggested 10 mL min Aspirate Flow flow rate values see Flow Rates on page 4 93 Rate ASPEC XL4 The speed at which the Volume moves out of the probe For a range of suggested 10 mL min Dispense Flow flow rate values see Flow Rates on page 4 93 Rate ASPEC XL4 The quantity of the air gap aspirated before the Volume 20 uL Air Gap ASPEC XL4 The speed at which the Air Gap moves into the probe 0 3 mL min Air Gap Flow Rate GX 274 The transfer port on the GX 274 to prime A Transfer Port GX 274 The zone from which the Volume is drawn Zone GX 274 The quantity of liquid aspirated for the prime OuL Volume Prime Transfer Ports Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide A 99 S 10d JajsueAL SWI Prime Transfer Ports Tasks Liquid Handling Prime Transfer Ports Properties Continued A Property Name Brief Description
100. Value Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Rinse the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Rinse Probes Inside Rinse Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide B 137 Saqojd asuly SS SS Rinse Probes Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Rinse Probes Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negati
101. Valve Position selected 4 Wait Equilibration Time 5 Perform a Touch Off X direction if selected and move Z to Z Safe Height 6 If Source from Tray and Source Volume is greater than Transfer Tubing volume repeat Aspirate Air Gap and Extra Volume not aspirated and Dispense until Source Volume is delivered 7 Move probe to Inside Rinse Well in Inside Rinse Zone 0 0 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset O Dispense Source Air Gap Extra Volume at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to Inside Rinse Well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 4 Switch solvent valve to Inside Rinse Solvent Valve Position 5 Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE 1 Move probe to first well in Outside Rinse Zone 2 Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset 3 Switch Peristaltic Rinse Pump on at Rinse Pump Speed TRILUTION LH Software Version 2 0 User s Guide B 87 su ds d SS SS Dispense Tasks SS Liquid Handling B 4 Switch solvent valve to Rinse Well 1 Solvent Valve Position 5 Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valv
102. Version 2 0 User s Guide TRILUTION LH Software Overview Utilities Utilities accesses another menu with options for setting units viewing run results purging and recovering items creating custom Bed Layouts registering TRILUTION LH and enabling ERM Menu Described On Unit Settings page 1 14 Run Results page 1 14 Purge and Recover page 1 15 Bed Layout and Utilities page 1 15 Register page 1 16 ERM page 1 16 Back Click the Back button a to return to the previous menu TRILUTION LH Software Version 2 0 User s Guide 1 13 Ssnu W H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 14 Unit Settings Members of the default Admin group can use Unit Settings to set the units of measurement for the Commands and Tasks and elsewhere in the software Only Users assigned to the Admin group have Unit Setting capabilities Unit Types are listed in the following table Unit Type XYZ Movement Speed of Movement Units of Measurement mm cm cm sec mm hr mm min mm sec Volume mL nL uL Flow Rate mL hr mL min mL sec uL min uL sec Time hrs min sec Pressure psi MPa Bar Temperature C F K WaveLength nm Mass mg Ug Run Results Use the Run Results utility window to review application run and or simulation run results The Utilities Run Results window provides the ability to view export archive restore and refresh the applica
103. WN 9 Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min TRILUTION LH Software Version 2 0 User s Guide 3 n J Elute Tasks SPE INSIDE RINSE mM A U N gt Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate Move Z to top OUTSIDE RINSE U A U N gt Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Height from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Move Z top GAS AIR PURGE If SPE 215 TRUE U A U N gt Move Z to top Set Voltage On and Output Contact 1 to Close Wait 0 02 min Set Voltage Off and Output Contact 1 to Open Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Evaporate This task evaporates liquids in the collect wells by passing pressurized gas or air from the syringes into the wells with the mobile rack positioned over the drain For more information about the properties on each tab see Evaporate Properties on page D 44 Evaporate Advanced Rinsing on page D 46 Eva
104. Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Syringe 1 2 Move probe to DEC Well in DEC Zone Move Z to top TRILUTION LH Software Version 2 0 User s Guide peoy Load Tasks SPE CON WN HR W 9 10 11 12 Val CON AO MN BP WN c 9 Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top Dispense Air Push Volume at Dispense Flow Rate Wait Equilibration Time Move Z to top performing shake off SPE 215 Repeat steps 4 9 until Air Push Volume is dispensed Move probe to Inside Rinse Wells at Inside Rinse Zone Dispense Air Gap at Dispense Flow Rate ve Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe
105. Well in Result Zone Lower probe into well to Result Height Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time U A U N Perform a Touch Off X direction if selected and move Z to Z Safe Height e If Source Volume is greater than syringe capacity repeat aspirate Air Gap and Extra Volume are not aspirated and dispense until Source Volume is delivered TRILUTION LH Software Version 2 0 User s Guide A 71 wopuey 0 Wopuey asuadsig Dispense Random to Random Tasks Liquid Handling 6 Move probe to well in Inside Rinse Zone 7 Lower probe into well to Inside Rinse Height 8 Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate 5 Dispense Inside Volume at Inside Rinse Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OU A U N gt TRILUTION LH Software Version 2 0 User s Guide Task
106. When Z 0 the bottom of the element is placed at the same level as the top of the template 5 Optionally select a value from the Rotation drop down Default is 0 Rotation values 90 180 and 270 Click ADD Confirm that the element has been added in the Preview 7 Click CLOSE to return to the Template TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities H Adding Multiple Footprints This option allows you to add an array of identical footprints to a template 1 2 3 4 Right click on a template and select ADD ELEMENTS Select the footprint that you want to add as an array Select the Multiple check box to enable the necessary fields Enter the X Y and Z Relative positions for the center of the element in column 1 row 1 The position is calculated with respect to the center of the template which may or may not be 0 0 0 depending on where the origin was set When X 0 and Y 0 the footprint in column 1 row 1 is centered over the center of the template Specify the number of columns and the number of rows Specify the distance between wells within a column Y Offset and the distance between wells within a row X Offset Optionally select a value from the Rotation drop down Default is 0 Rotation values 90 180 and 270 Click ADD Confirm that the elements were added in the Preview Click CLOSE to return to the Template TRILUTION LH Software Version 2 0
107. XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Oo ON WD MN B Syringe Move probe to DEC Well in DEC Zone Move Z to top Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top N OW A U N gt Dispense Air Push Volume at Dispense Flow Rate D 16 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 8 Wait Equilibration Time 9 Move Zto top performing shake off SPE 215 10 Repeat steps 4 9 until Air Push Volume is dispensed 11 Move probe to Inside Rinse Wells at Inside Rinse Zone 12 Dispense Air Gap at Dispense Flow Rate Valve Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top CON AO WM BP WN 9 Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min TRILUTION LH Software Version 2 0 User
108. Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Add Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide PPV SS SS Add Tasks SS Liquid Handling SS Add Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse S
109. Z to Z Z Safe Height b Move probe to Result Well in Result Zone same as in Dispense c Aspirate Mix Air Gap at Mixing Flow Rate d Lower probe into well to Result Height e Aspirate Mixing Volume at Mixing Flow Rate using Liquid Level Following if selected f Lower probe into well to Result Height g Dispense Mixing Volume at Mixing Flow Rate using Liquid Level Following if selected h Wait Equilibration Time A 42 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling i Repeat steps d h until Number of Mixes complete j Perform a Touch Off X direction if selected and move Z to Z Safe Height 2 Drain a Move probe to well in Inside Rinse Zone b Lower probe into well to Inside Rinse Height c Dispense Mix Air Gap at Mixing Flow Rate d Move Z to Z Safe Height MIX WITH AIR 1 Aspirate a Move Z to Z Safe Height b Move probe to Result Well in Result Zone same as in Dispense c Aspirate Mix Air Gap at Mixing Flow Rate d Move Z to Z Safe Height e Aspirate Mixing Volume at Mixing Flow Rate f Lower probe into well to Result Height g Dispense Mixing Volume at Mixing Flow Rate h Wait Equilibration Time i Repeat steps d h until Number of Mixes complete j Perform a Touch Off X direction if selected and move Z to Z Safe Height INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone TRILUTION LH Software Version 2 0 User s Guide Z ea q Derivatize Ta
110. a new Application PASTE APPLICATION Paste a copied Application in the current folder Folder Right click on a folder in the Applications palette and the following options are displayed Menu Description NEW FOLDER Displays the New Folder dialog for creating a new folder in the current folder RENAME FOLDER Rename the current folder DELETE FOLDER Deletes the folder if empty TRILUTION LH Software Version 2 0 User s Guide Application Menu Description NEW APPLICATION Create a new Application PASTE APPLICATION Paste a copied Application in the current folder Application Right click on an Application and the following options are displayed Menu Description NEW APPLICATION Create a new Application OPEN APPLICATION Open an existing Application DELETE APPLICATION Deletes the selected Application or all versions of the Application COPY APPLICATION Copies the Application for pasting in another folder TRILUTION LH Software Version 2 0 User s Guide 8 5 MOpul UO ed1 ddy Application Window Application 8 6 Sample List The Sample List lists the steps to execute during a run Choose available functions using the icons in the Sample List workspace icon Description F Imports Sample List TSL files F Exports the Sample List to a selected location as a TSL file l g Set initial volumes for any or all wells in each Bed Layout in the Application E
111. acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered Result Flow Rate The speed at which the Solution Volume moves into the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Dispense Random to Random with Tips Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide C 19 sdi yyIM WopueyY 0 wopuey ssuadsig Dispense Random to Random with Tips Tasks Disposable Tips Dispense Random to Random with Tips Properties Continued Property Name Brief Description Default Value Equilibration Time the program waits after each aspirate and dispense before moving out of 0 min Time the well Touch Off Select Touch Off to activate the Side or Surface Touch Off on the Tips tab Cleared Dispense Random to Random with Tips Properties Table Page 2 of 2 Dis
112. and tracking audit trails electronic signatures and method versioning of records as well as permitting customized user access levels within the software This menu accessible only to members of the default Admin group provides options for electronic record management ERM Features This menu is accessed from the ERM menu by a member of the default Admin group ERM is enabled from this menu by selecting the Enable ERM Features check box TRILUTION LH defaults with this box cleared ERM features disabled TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview TRILUTION LH provides for the option to enable signature points each time a component is saved Enable this option by first selecting to Enable ERM Features and then select the Enable Signature Points check box The Enable Signature Points defaults cleared no signatures required when saving Audit Trail This menu is accessed from the ERM menu by a member of the default Admin group The Audit Trail dialog offers three options Audit Trail Event Log and E Signature Audit Trail records the details of changes made in any of the builders prior to a save Double click the event in the Audit Trail to display the details of the changes or click PREVIEW to view the information in a report format Event Log records the click of any action button in all builders E Signature records the details including comments entered of each e signature p
113. and how to generate the Sample List Use the Application window to start and monitor the progress of a run To perform manual control use the Manual Control as described in Appendix 9 Application Manual Control TRILUTION LH Software Version 2 0 User s Guide 8 1 Application 8 Application Window Access the Application window by e clicking APPLICATION from the Liquid Handling menu e clicking RUN in the Method Builder Application Window The Application Window 8 2 TRILUTION LH Software Version 2 0 User s Guide Application The Application Window has the following sections Section Described on Title Bar page 8 3 Applications Palette page 8 3 Sample List page 8 6 Bed Layout View page 8 8 Simulation page 8 11 Run Indicator page 8 11 Pressure Indicator page 8 11 Action Buttons Application page 8 12 Info Window page 8 13 Title Bar The Title Bar at the top displays the names of the running Application and the logged on User Applications Palette Lists the Applications created TRILUTION LH Software Version 2 0 User s Guide 8 3 MOpul UO ed1 ddy Application Window Application 8 4 Right click Menu Application Applications palette Right click in the Applications palette and the following options are displayed Menu Description NEW FOLDER Displays the New Folder dialog for creating a new top level folder NEW APPLICATION Create
114. as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Dispense Random to Random Advanced Outside Rinse Table Page 2 of 4 Omm TRILUTION LH Software Version 2 0 User s Guide B 95 wopuey 0 wopuey su ds q SS SS Dispense Random to Random Tasks SS Liquid Handling SS Dispense Random to Random Advanced Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired posit
115. as it is aspirated This Following selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tubes This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Load and Collect Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Load and Collect Advanced Rinsing Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when delivering to the DEC Zone Air Push Height The distance up when
116. as it is aspirated This Following selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tubes This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Condition Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide D 9 UO puo gt Condition Tasks SPE D Condition Advanced Rinsing Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when delivering to the DEC Zone Air Push Height The distance up when a po
117. before any changes become effective TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility K The GSIOC Utility allows you to issue commands to Gilson GSIOC instruments Your Gilson authorized representative may ask you to use this utility to verify that an instrument is connected correctly to the computer For communication to occur the Gilson instrument must be connected via an RS 232 connection to the computer or connected via a GSIOC connection to a Gilson interface instrument that is connected to the computer TRILUTION LH Software Version 2 0 User s Guide K 1 GSIOC Utility GSIOC Utility K 2 Starting the GSIOC Utility 1 Locate the GSIOC Utility GSUTIL32 EXE using Windows Explorer or the shortcut at START gt ALL PROGRAMS gt GILSON APPLICATIONS gt UTILITIES gt GSIOC UTILITY During installation this utility was stored to C GILSON UTIL unless the installation path was changed 2 Start the utility The GSIOC Utility window appears i Gilson GSIOC Utility it Mode Help GSIOC Server 1 03 Port COM1 Unit ID v IRQ N A l Immediate l Buffered Baud 19200 Command 4 Response Reviewing the Port and Baud Information In the GSIOC Utility window review the COM port and baud information If any information is incorrect or missing close the GSIOC Utility and use the GSIOC Configuration Editor to update the information Refer to Appendix J GSIOC Configuration Editor TRILUT
118. calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Dispense Random to Random with Tips Tips Table Page 3 of 3 Dispense Random to Random with Tips Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Active Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Source Height The distance up when a positive number is entered or down when a negative 2 mm number is entered from a defined reference point Tube Bottom unless Liquid Level Following is selected when aspirating the Source Volume Dispense Random to Random with Tips Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Dispense Random to Random with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Following is sele
119. error The default is No Change for Pee Outputs and Open for 24V Outputs 24V Output 4 Input A Optionally type a description for the N A N A Input B contact This description will be used to Input C identify the contact in a Task Input D GX 281 Liquid Handler Properties Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide 4 67 uoljeinbiyuo e Gules Creating a Configuration Method Builder Configuration GX 281 Liquid Handler Properties Continued General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task GSIOC ID A unique numerical address that must be 25 20 29 known to the instrument and to the software that allows the software to communicate to the instrument GX 281 Liquid Handler Properties Table Page 4 of 4 4 68 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Minipuls 3 ID ChannelName Tubing ID mm Max Flow Rate ml min Reservoir Name gt A A None Reservoir The Minipuls 3 Properties Window Properties Minipuls 3 Properties Property Name Brief Description Default Value Range Setup Aspiration Direction Specifies which way the pump head on Counter Clockwise the Minipuls is turning when it is Clockwise Counter Clockwise aspirating Minipuls 3 Properties Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 4
120. ew e buimai Saving a Method Method Builder 3 6 Saving a Method 1 Click SAVE The Save Method window appears gi Save Method The Save Method window 2 On the Save Method window do the following a Inthe Name field type a unique Method name b In the Configuration Name field type a Configuration Name Optionally select the Set as Default check box to set this Configuration as the default for all new Methods c In the Bed Layout Name field type a Bed Layout Name If the Configuration was set as default this will be the default Bed Layout associated with that Configuration TRILUTION LH Software Version 2 0 User s Guide Method Builder d In the Error Name field type a name for the error handling parameters Optionally select the Set as Default check box to set these parameters as the default for all new Methods e In the Description field optional type a brief description of the Method TRILUTION LH Software Version 2 0 User s Guide 3 7 pou ay e Burnes Exporting Methods Method Builder 3 8 Exporting Methods 1 On the Method Builder click EXPORT The Select items to Export window displays all the available Methods EJ Select items lo Export Select the items to be en ans kc is Et Example Method The Select items to Export window 2 Inthe Select items to Export window complete the following a In the Select the items to be exported field select the Method s
121. fills the outside rinse wells with liquid from the pump reservoir Properties Fimstruments Prime Rinse Station Rinsing Parameters Rinse Touch Off Mode Side Surface A Ovtside Rinse Zone tk Well Outside Volume ut Outuide Flow Rate mifimin s 10 Ovtsiderinserteght mm is None For more information about the properties on each tab see Prime Rinse Station Properties on page C 68 Prime Rinse Station Instruments on page C 70 _ Prime Rinse Station Sequence of Steps on page C 70 ee oK Camel Help a i Prime Rinse Station Properties Property Name Brief Description Default Value Outside Rinse The zone to which the Outside Volume is delivered Outside Rinse Zone Well Enter the well number in the zone 1 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Prime Rinse Station Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Prime Rinse Station Properties Continued Property Name Brief Description Default Value Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube B
122. for subsequent result wells when a variable is used for Result Well Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Source Volume at the Result Flow Rate using Liquid Level Following if selected B 42 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height e If Source Volume is greater than Transfer Tubing volume repeat aspirate Air Gap and Extra Volume are not aspirated and dispense until source volume delivered 7 Move Z to Z Safe Height 8 Move probe to Inside Rinse Well in Inside Rinse Zone 9 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 10 Dispense Extra Volume Source Air Gap at the Result Flow Rate 11 Move Z to Z Safe Height INSIDE RINSE Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Switch solvent valve to Inside Rinse Solvent Valve Position mM A U N gt Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE 1 Move probe to first well in Outside Rinse Zone 2 Lower probe into well to Outside Rinse Z Option and Outside Rinse Z O
123. from Reservoir at Result Flow Rate 6 Wait Equilibration Time D 36 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 7 Move Z to top If Source Volume is greater than Syringe Volume repeat steps 2 7 until Source Volume delivered 8 If SPE 215 Shake Off TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 Shake Off FALSE a Move Z to top Tray Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into Source Well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected mM A U N gt Move Z to top Transfer Port Aspirate Source Air Gap at Air Gap Flow Rate Move probe to well in Source Zone Lower probe to Source Height from Tube Bottom Set Transfer Port to On Aspirate Source Volume Extra Volume at Source Flow Rate U A U N gt TRILUTION LH Software Version 2 0 User s Guide 3 n J Elute Tasks SPE 6 7 Set Transfer Port to Off Move Z to top DISPENSE Tray 7 8 9 Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time Move Z to top e If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume delivered If SPE 215 Shake Off TR
124. in Source Zone 3 Lower probe to Source Height from Tube Bottom 4 Aspirate Source Volume Extra Volume at Source Flow Rate 5 Move Z to top DISPENSE Tray 1 Move probe to DEC Well in DEC Zone 2 Lower probe to Result Height from Tube Top 3 Dispense Source Volume at Result Flow Rate TRILUTION LH Software Version 2 0 User s Guide 9 euU01 e114 Fractionate Tasks SPE D Wait Equilibration Time Move Z to top Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Dispense Extra Volume Air Gap at Result Flow Rate Oo CON WD MN RA Move Z to top Transfer Port Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time MOM A WN gt Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume delivered Move probe to Inside Rinse Zone in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Dispense Extra Volume Air Gap at Result Flow Rate O CO N OV Move Z to top D 62 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE AIR PUSH Solenoid 1 2 3 Oo ON WD MN RA Move probe to well in Inside Rinse Zone Aspirate 250 uL at 5 mL min Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min S
125. in the Sample List If a variable is not 1 specified all four probes will go to well 1 Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Cherry Pick with Tips Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Cherry Pick with Tips Properties Continued Property Name Brief Description Default Value Equilibration Time the program waits after dispense before moving out of the well 0 min Time Touch Off Select Touch Off to activate the Side or Surface Touch Off on the Tips tab Cleared Cherry Pick with Tips Properties Table Page 2 of 2 Cherry Pick with Tips Tips Property Name Brief Description Default Value Tip Waste Zone The zone assigned to the tip chute at which the tips are ejected Load Tips Before Select and tips will be loaded at the beginning of the task Selected Task Clear and tips will not be loaded If tips were previously loaded by another task clear this option Eject Tips After Select and all tips will be ejected before the rinses Selected Task Clear and the tips will remain loaded Prewet Tip Select this option to wet the tip before aspirating the Source Volume 1st Iteration Cleared only Prewet Volume The quantity of liquid source aspirated and dispensed to prewet the tip when 10 u
126. into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Flow Rate Dispense Inside Volume at Inside Flow Rate Repeat steps 4 5 until Inside Volume delivered OUTSIDE RINSE OPTIONAL nun A U N gt Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate Repeat steps 4 5 until Outside Volume delivered RINSE TOUCH OFF Side Move Z to top Move probe to well in Inside Rinse Zone Move probe to Side Rinse Z Offset Move probe 1 5 mm in the Y direction forward Move probe to Side Rinse Y Offset TRILUTION LH Software Version 2 0 User s Guide C 43 sdi y m asuadsig Dispense with Tips Tasks Disposable Tips 6 7 Move probe to the center of the well Move Z to top Surface 1 2 3 4 Move Z to top Move probe to well in Outside Rinse Zone Move probe to Surface Rinse Z Offset Move Z to top None 1 C 44 Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Eject Tips This task ejects tips to the tip waste zone _ For more information about the properties on each tab see Eject lips Eject Tips Properties on page C 45 Tip Disposal wp Tip Waste Zone Sowce Zone gt Eject Tips Instruments on page C 46 Eject Tips Sequence of Steps on page C 4
127. is selected the Audit log is enabled When Enable Signature Points is selected a signature will be required by every user for every save These signatures will be tracked in the E Signature log For information on how to set up users and groups See Users and Groups in Chapter1 TRILUTION LH Software Version 2 0 User s Guide Electronic Record Management ERM Features Users and Groups During the TRILUTION LH installation by default two groups are created Admin and Analyst Administrator is the default user in the Admin group The Admin group is assigned all rights Analyst is the default user in the Analyst group The Analyst group can create modify view and run applications in TRILUTION LH Create Modify Delete View Import Export Run Analyst Create Modify View Run Default Permissions for Default Groups TRILUTION LH Software Version 2 0 User s Guide sainjea WY JUaWebeuew psoray 21U0172 43 Users and Groups Electronic Record Management ERM Features l The graphic below depicts which permissions are required for a basic workflow The permissions are listed first followed by the builder in which those permissions must be granted followed by the action How User Permissions Integrate with a Basic TRILUTION LH Workflow i E 3 m u Q E Application l 4 TRILUTION LH Software Version 2 0 User s Guide Electronic Record Management ERM Features
128. is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone SS Dispense Advanced Inside Rinse Table Page 1 of 3 2mm TRILUTION LH Software Version 2 0 User s Guide su ds d SS SS Dispense Tasks SS Liquid Handling SS Dispense Advanced Inside Rinse Continued Property Name Brief Description Default Value Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse V
129. list with the same Unit ID and command Using the button 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to Type a Command and Comment The comment is optional Click the G button next to the Immediate button The new command will be inserted at the bottom of the command list Note The command will not be inserted if there is already a command line in the list with the same Unit ID and command TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility K Insert a Buffered Command There are two ways to insert a buffered command in Advanced mode Using the Edit menu 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to Type a Command and Comment The comment is optional Select Insert Immediate from the Edit menu The new command will be inserted at the bottom of the command list Note The command will not be inserted if there is already a command line in the list with the same Unit ID and command Using the button 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to Command Type Type a Command and Comment The comment is optional Click the button next to the Buffered button The new command will be inserted at the bottom of the command list Note The command will not be inserted if there is already a command line in the list with the same Unit ID and command
130. moving in the X or Y direction ASPEC XLi Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 45 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration Property Name Contacts ASPEC XLi Properties Continued Brief Description Default Value Range Relay Output 1 Relay Output 2 Relay Output 3 Output 4 Output 5 Output 6 Output 7 Output 8 Optionally type a description for the contact This description will be used to identify the contact in a Task N A N A Analog Input Input 1 Input 2 Input 3 Input 4 Optionally type a description for the contact This description will be used to identify the contact in a Task N A N A General Instrument Name The name that will be used to identify this instrument in a Task Liquid Handler N A GSIOC ID A unique numerical address that must be known to the instrument and to the software that allows the software to communicate to the instrument ASPEC XLi Properties Table Page 2 of 2 10 0 31 4 46 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 271 ASPEC without Pump Not Installed The GX 271 ASPEC without Pump Properties Window TRILUTION LH Software Version 2 0 User s Guide s ae 4 47 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration Properties
131. negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Rinse Tip Holders Properties Table Page 2 of 2 Rinse Tip Holders Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Rinse Tip Holders Instruments TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Rinse Tip Holders Sequence of Steps The following is an overview for the sequence of steps 1 Inside Rinse Optional on page C 75 2 Outside Rinse Optional on page C 75 3 Rinse Touch Off on page C 76 INSIDE RINSE OPTIONAL 1 Move Z to top 2 Move probe to well in Inside Rin
132. of valid immediate commands for each instrument in its user s guide Buffered button Sends a buffered command to the Unit ID selected Buffered commands send instructions to an instrument These commands are executed one at a time Each command is a string of no more than 40 characters You can find a list of valid buffered commands for each instrument in its user s guide Command field Where the command to be sent is specified For more information on sending commands see Send an Immediate Command on page K 24 or Send a Buffered Command on page K 25 TRILUTION LH Software Version 2 0 User s Guide K 5 AINN DOISD GSIOC Utility GSIOC Utility K 6 Response field Returns a response to an immediate or buffered command The response to a successfully completed buffered command is ok Refer to the user s guide for the Gilson instrument for a description of the valid response to immediate commands The response to an unsuccessfully completed immediate or buffered command is error TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility Basic Mode Menus Mode Menu There are three options in the Mode menu Scan Advanced and Ghost Scan The Scan option on the Mode menu allows you to scan for GSIOC instruments After a scan the Unit ID list box displays the unit IDs and the version of the connected instruments If any connected instruments are missing from the list ensure that t
133. on screen Z Safe Height mm The Z height to which the instrument s 207 Calculated based probe moves before moving in the X or Y on Z Clamp Height direction and Probe Type Range provided on screen Transfer Tubing mL The size of the tubing being used from 30 1 1 3 5 10 5 30 50 pump to probe Other Tubing mL When selected the non standard size of 0 0 99 the tubing being used from pump to probe Left GX Direct Inject Valve Indicate the size of the valve installed inthe Not Installed Not Installed left position 1 8 1 16 Right GX Direct Inject Valve Indicate the size of the valve installed in the Not Installed Not Installed right position 1 8 1 16 GX 281 Liquid Handler Properties Table Page 2 of 4 4 66 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 281 Liquid Handler Properties Continued Property Name Brief Description Default Value Range Waste Indicate the solvent valve port to be used Port 1 Port 1 Port 2 Port 3 for waste Port 4 Port 5 Port 6 Port 1 Optionally type a description of the solvent N A N A Port 2 valve port Port 3 Port 4 Port 5 Port 6 Contacts Output 1 Optionally type a description for the N A N A Output 2 contact This description will be used to Output 3 identify the contact in a Task aS 3 i Additionally indicate whether the contact JAN oR 5 should open close or remain in the same JAN ARP 3 state on
134. point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Add Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide PPV SS SS Add Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Add Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Add Outside Rinse Table Page 1 of 4 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Add Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse
135. positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Prime Pump Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 129 duing Wld SS SS Prime Pump Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Prime Pump Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Prime Pump Outside Rinse Table Page 1 of 4 B 130 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Prime Pump Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level
136. properties right click on the element and select PROPERTIES 2 Select the Advanced check box to view the attributes for the element The relative coordinates display The coordinates are relative to the center of the holder TRILUTION LH Software Version 2 0 User s Guide s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities H Moving Single Elements 1 Right click on the element and select Properties 2 Change the appropriate position coordinates Deleting Elements You can only delete user defined elements 1 Open the Rack Elements dialog right click on an element then select ADD ELEMENTS 2 Click on the type of custom element that you want to delete a footprint well or tip 3 Select the specific element you want to delete from the list Click DELETE H 18 TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Deleting a Rack 1 Click DELETE RACK The Delete Rack window appears Delete Hack 3M Empore NAME ME 500 mL Battle 700 mL Bottle AFR Appled Seperatan ustOMix A et tt The Delete Rack Window 2 Select the rack to be deleted and click DELETE If more than one version of the rack exists TRILUTION LH will confirm that you want to delete all versions of the rack TRILUTION LH Software Version 2 0 User s Guide s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities H Exporting Racks and Templates Click EXPOR
137. quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source Volume is delivered When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Dispense Random to Random Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide A 67 wopuey 0 Wwopuey su ds q Dispense Random to Random Tasks Liquid Handling Dispense Random to Random Properties Continued A Property Name Brief Description Default Value Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Dispense Random to Random Properties Table Page 2 of 2 Dispense Random to Random Advanced Rinsing Property Name
138. s Guide TRILUTION LH Software Overview To open Users and Groups 1 On the TRILUTION menu click ADMINISTRATIVE TOOLS 2 Onthe Administrative Tools menu select USERS AND GROUPS option The Users and Groups window displays Users and Groupes The Users and Groups window has two frames e The left frame provides two sections a Users Create and modify Users b Groups Create and modify Groups TRILUTION LH Software Version 2 0 User s Guide 1 21 snuaW H7 NOLLATYL TRILUTION LH Menus TRILUTION LH Software Overview 1 22 1 The right frame displays the information based on the Tab selected a On selecting the Users tab the Group name and Group description display b On selecting the Groups tab the User Name and User Description display Only those Users within the Admin group can create modify or delete Users and Groups TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Groups Users and Groups The Groups tab A Group combines Users with common roles Each role has assigned permissions The Groups tab lists all the available Groups You can create modify and delete Groups On selecting a Group from the group tab the Users belonging to that Group display in the right frame To create a Group 1 On the Users and Groups window click GRoupPs tab The name of the default Groups Admin and Analyst display in the left f
139. see Cherry Pick with Tips Properties on page C 6 Cherry Pick with Tips Tips on page C 7 Cherry Pick with Tips Advanced Rinsing on page C 9 Cherry Pick with Tips Instruments on page C 11 Cherry Pick with Tips Sequence of Steps on page C 12 TRILUTION LH Software Version 2 0 User s Guide C 5 SAIL YUM 491d A1134 Cherry Pick with Tips Tasks Disposable Tips Cherry Pick with Tips Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn 1 Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source Volume is delivered Starting Well Type x where x is a variable value defined
140. speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inject Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Inject Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Inject Advanced Rinsing Table Page 3 of 3 Inject Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal d
141. support for international locale settings TRILUTION LH Software Version 2 0 User s Guide 1 1 Unpacking TRILUTION LH TRILUTION LH Software Overview 1 Unpacking TRILUTION LH Unpack TRILUTION LH upon receipt even if the software will not be used immediately Many carriers must receive concealed damage claims within seven days of delivery TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview 1 Customer Service Gilson Inc and its worldwide network of authorized representatives provide customers with the following types of assistance sales technical support applications and instrument repair If you need assistance please contact your Gilson authorized representative Specific contact information can be found on the Gilson website at www gilson com Before Calling Us Gilson authorized representatives will be able to serve you more efficiently if you have the following information Serial number and model number of the Gilson instruments involved e Installation procedures that were used e List of concise symptoms e List of operating procedures and conditions you were using when the problems arose e List of other instruments connected to the system and a description of those connections List of other electrical connections in the room TRILUTION LH Software Version 2 0 User s Guide 1 3 S JawWO sN gt DIIAIO Training TRILUTION LH Software Overview 1 Tr
142. surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone SS Transfer Advanced Inside Rinse Table Page 1 of 3 2mm B 148 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Transfer Advanced Inside Rinse Continued Property Name Brief Description Default Value Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The positi
143. that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Fractionate Instruments D 59 TRILUTION LH Software Version 2 0 User s Guide 9 euU01 Ie14 Fractionate Tasks SPE D Fractionate Sequence of Steps The following is an overview for the sequence of steps Move Mobile Rack on page D 60 Aspirate on page D 60 Dispense on page D 61 Air Push on page D 63 Inside Rinse on page D 65 Outside Rinse on page D 65 N OW A U N gt Gas Air Purge on page D 65 MOVE MOBILE RACK 1 Move Z to top 2 Move mobile rack DEC Zone over the specified collect zone ASPIRATE Reservoir 1 Move Z to top 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to DEC Well in DEC Zone 4 Lower probe to Result Height from Tube Top 5 Dispense Source Volume from Reservoir at Result Flow Rate D 60 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 6 Wait Equilibration Time 7 Move Z to top If Source Volume is greater than Syringe Volume repeat steps 2 7 until Source Volume delivered Tray Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone 1 2 3 Lower probe into Source Well to Source Height 4 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 5 Move Z to top Transfer Port 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well
144. the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tubes This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Wash Advanced Rinsing Table Page 1 of 3 D 106 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Wash Advanced Rinsing Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when delivering to the DEC Zone Air Push Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to ri
145. the VALVEMATE from the drop down list b In the Position field click the arrow and then select the VALVEMATE valve position from the drop down list that corresponds to the first reservoir solution c Inthe Pump Name field type a name for the first VPS This is a good place to reference the first reservoir solution This name will be used to identify the VPS in a Task Repeat step 3 for additional reservoirs TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Quad Z 215 with Two 402 Pump Duals For asystem using multiple syringe sizes with a Quad Z 215 complete the following 1 Drag and drop a Quad Z 215 into the workspace and set the properties Drag and drop two 402 Pump Duals into the workspace and set the properties You will need to set a unique GSIOC ID for each Drag and drop a Virtual Pumping System into the workspace a Click on a Syringe Name and then click the up or down arrow to associate the syringe with a probe b Inthe Pump Name field type a name for the VPS This name will be used to identify the VPS in a Task Single Probe Multiple Syringe Configuration 1 Drag and drop a single probe liquid handler into the workspace and set the properties Drag and drop a VALVEMATE into the workspace and set the properties Your VALVEMATE should be plumbed and ready to go at this point Drag and drop the pumps into the workspace and set the properties You will need to set a u
146. the center of the template c To zoom out on the Template click once or repeatedly 5 Click OK Click SAVE AS TRILUTION LH Software Version 2 0 User s Guide H 3 s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities H 7 Enter the template name description and short description in the Save As Template window 8 Click OK Origin Length 590 mm e Origin X Origin 0 Y Origin 0 Z Origin 0 Origin X Origin 0 Y Origin 0 Z Origin 0 TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Length 590 mm Width 330 mm Move X 285 and Y 165 0 0 0 Origin 285 165 0 Absolute X Y Z Origin Default Settings Applied TRILUTION LH Software Version 2 0 User s Guide s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities Adding Footprints A footprint is an area that accepts racks Gilson supplies footprints or create custom footprints Gilson supplied footprints cannot be modified or deleted Adding Single Footprints 1 Click on the template it highlights in red 2 Right click and select ADD ELEMENTS 3 Select a footprint 4 Enter the X Y and Z Relative positions The position is calculated with respect to the center of the template which may or may not be 0 0 0 depending on where the origin was set When X 0 and Y 0 the center of the element is situated over the center of the template
147. the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Dilute Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide B 69 7N d SS SS Dilute Tasks SS Liquid Handling SS Dilute Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Positi
148. the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Prime Pump Advanced Rinsing Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide A 95 duing Wld Prime Pump Tasks Liquid Handling Property Name Liquid Handler Prime Pump Instruments Brief Description The liquid handler that the task will affect A Default Value Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Prime Pump Instruments Prime Pump Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 96 2 Inside Rinse on page A 97 3 Outside Rinse on page A 97 ASPIRATE 1 Move Z to Z Safe Height 2 Aspirate Prime Volume from Reservoir at Source Flow Rate 3 Move probe to Result Well in Result Zone 4 Lower probe into well to Result Height A 96 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling 5 6 Dispense Prime Volume at Result Flow Rate Repeat steps 2 5 until Prime Volume has been delivered INSIDE RINSE N OW A WU N gt Move Z to Z Safe Height Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspi
149. then dispense the volume to the wells of the DEC zone with the mobile rack positioned over the drain Properties Advanced Rtining flinstruments l For more information about the properties on each tab see Load 00 Load Properties on page D 68 Load Advanced Rinsing on page D 72 Load Instruments on page D 74 Load Sequence of Steps on page D 75 TRILUTION LH Software Version 2 0 User s Guide D 67 peoy Load Tasks SPE D Load Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into and out of the probe For a 3 mL min Source Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volu
150. this course participants will be able to e Control a Gilson liquid handler from TRILUTION LH e Create a bed layout representing the positions of samples mixing vials reagents standards etc e Create automated methods for dilution microplate replication communication with a non Gilson device etc e Understand the importance of the system configuration in building methods and controlling systems and running applications with one or more configuration Incorporate variables for method flexibility e Simulate an application to check a method for errors e View and understand log files Advanced TRILUTION LH Training TRTRILUTION2 2 days This advanced training course builds on the information presented in the introductory training course TRTRILUTION1 Participants spend two days creating custom tasks custom racks and running advanced exercises and applications specific to their needs Following training participants will be considered advanced users with a thorough understanding of TRILUTION LH operation TRILUTION LH Software Version 2 0 User s Guide 1 5 Buiuieay Training TRILUTION LH Software Overview 1 Course Objectives After attending this course participants will be able to e Customize racks within TRILUTION LH e Interface TRILUTION LH with other software through custom tasks and or contact closure e Understand and utilize operators to add flexibility to tasks and methods variables expres
151. to Valve Volume at 10 mL min INSIDE RINSE Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate mM A WN gt Move Z to top OUTSIDE RINSE Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Height from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate mM A U N gt Move Z top GAS AIR PURGE If SPE 215 TRUE 1 Move Z to top 2 Set Voltage On and Output Contact 1 to Close 3 Wait 0 02 min TRILUTION LH Software Version 2 0 User s Guide D 81 peoy D 82 Tasks SPE 4 Set Voltage Off and Output Contact 1 to Open 5 Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load and Collect If the contaminants bind to the column and the analytes of interest pass through the column into the collect zone use this task to aspirate the specified volume of liquid source from the reservoir tray or transfer ports and then dispense the volume to the wells of the DEC zone with the mobile rack over the collect position For more information about the properties on each tab see Load and Collect Properties on page D 84 Load and Collect Advanced Rinsing on page D 88 Load and Collect Instruments on page
152. to Z Safe Height 11 Move probe to Inside Rinse Well in Inside Rinse Zone 12 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 13 Dispense Mix Air Gap Volume at Mixing Flow Rate INSIDE RINSE Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Switch solvent valve to Inside Rinse Solvent Valve Position mB WN gt Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE 1 Move probe to first well in Outside Rinse Zone 2 Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset 3 Switch Peristaltic Rinse Pump on at Rinse Pump Speed 4 Switch solvent valve to Rinse Well 1 Solvent Valve Position TRILUTION LH Software Version 2 0 User s Guide B 59 9ZILALIBG SS SS Derivatize Tasks SS Liquid Handling B 5 Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume 6 Switch Peristaltic Rinse Pump off 7 Move Z to Z Safe Height 8 Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station B
153. to the 29 20 29 instrument and to the software that allows the software to communicate with the instrument 819 Injection Module Properties TRILUTION LH Software Version 2 0 User s Guide 4 39 uoleinbyuo e buljeal gt Creating a Configuration Method Builder Configuration ASPEC XL The ASPEC XL Properties Window Properties ASPEC XL Properties Property Name Brief Description Default Value Range Setup Z Safe Height mm The Z height at which the instrument s probe moves to before 0 183 to 0 moving in the X or Y direction ASPEC XL Properties Table Page 1 of 2 4 40 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Property Name Contacts ASPEC XL Properties Continued Brief Description Default Value Range Relay Output 1 Relay Output 2 Relay Output 3 Output 4 Output 5 Output 6 Output 7 Output 8 Optionally type a description for the contact This description will be used to identify the contact in a Task N A N A Analog Input Input 1 Input 2 Input 3 Input 4 Optionally type a description for the contact This description will be used to identify the contact in a Task N A N A General Instrument Name The name that will be used to identify this instrument in a Task Liquid Handler N A GSIOC ID A unique numerical address that must be known to the instrument and to the softw
154. uoleinbyuos e Guljeai gt Creating a Configuration Method Builder Configuration 221 XL Liquid Handler Properties Continued Property Name Brief Description Default Value Range Contacts Relay Output 1 Optionally type a description for the contact N A N A Relay Output 2 This description will be used to identify the Relay Output 3 contact in a Task Output 4 Output 5 Output 6 Output 7 Output 8 Analog Input Optionally type a description for the contact N A N A Input 1 This description will be used to identify the Input 2 contact in a Task Input 3 Input 4 General Instrument Name The name that will be used to identify this instrument in a Task Liquid Handler N A GSIOC ID A unique numerical address that must be known to the instrument and to the software that allows the software to communicate with the instrument 221 XL Liquid Handler Properties Table Page 2 of 2 10 0 31 4 16 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 4 222 XL Liquid Handler The 222 XL Liquid Handler Properties Window Properties 222 XL Liquid Handler Properties Property Name Brief Description Default Value Range Setup Z Arm Type mm This is the height of the Z arm installed on the liquid 123 mm Z Arm 56 mm Z Arm handler 123 mm Z Arm 183 mm Z Arm N Z Safe Height mm The Z height to which the instrument s probe moves
155. used Prime Volume The quantity of the prime OuL Result Zone The zone to which the Prime Volume is delivered Inside Rinse Result Well The well in the Result Zone to which the Prime Volume is delivered 1 Result Flow Rate The speed at which the Prime Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 SS Prime Pump Properties SS Prime Pump Advanced Inside Rinse Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the Tube Bottom when dispensing to the Result Zone Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone SS Prime Pump Advanced Inside Rinse Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide B 127 duing Wld SS SS Prime Pump Tasks SS Liquid Handling SS Prime Pump Advanced Inside Rinse Continued Property Name Brief Description Default Value Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Ins
156. will be installed C 48 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Mix with Tips This task aspirates air or liquid and then dispenses it into a well a specified number of times For more information about the properties on each tab see Mix with Tips Properties on page C 50 Mix with Tips Tips on page C 52 Mix with Tips Advanced Rinsing on page C 54 Mix with Tips Instruments on page C 55 Mix with Tips Sequence of Steps on page C 56 TRILUTION LH Software Version 2 0 User s Guide C 49 sd L YIM XIW Mix with Tips Tasks Disposable Tips Mix with Tips Properties Property Name Brief Description Default Value Mix Zone The zone in which the Mixing Volume is mixed Mix Well The well in the Mix Zone in which the mix is performed Type x where x is a 1 variable value defined in the Sample List If a variable is not specified all four probes will go to well 1 Number of Type the number of times the Task should repeat the commands related to 1 Mixes mixing Mix with Air Mix Select Mix with Air or Mix with Liquid Mix with with Liquid Liquid Mix with Liquid The quantity of liquid used as part of a mixing process OuL Mix Volume Mix with Liquid The quantity of an air gap 20 uL Air Gap Mix with Liquid The speed at which the Mixing Volume moves into the probe For a range of 5 mL min Flow Rate suggested flow rate values see Flow Rates on p
157. 0 01 on j Cancel help Set Valve Position Sequence of Steps on page F 19 Set Valve Position Properties For more information about the properties on each tab see Property Name Brief Description Default Value Valve Type Select Solenoid or VALVEMATE Solenoid Solenoid The instrument type that the command will affect Instrument Set Valve Position Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide F 17 UOIUSOd AJLA 39S Set Valve Position Tasks Auxiliary Set Valve Position Properties Continued Property Name Brief Description Default Value Solenoid The output contact on the instrument that controls the Solenoid Valve Contact 1 Contact range 1 10 Solenoid Closes and powers on the specified output contact Closed On Solenoid Opens and powers off the specified output contact Open Off Solenoid Pulses the specified output contact Default Pulse Solenoid The time the program will wait between output state toggle Displays when Pulse 0 01 min Pulse Duration is selected for Contact Status VALVEMATE The VALVEMATE on which to set the valve position Instrument Name VALVEMATE Valve position 1 Valve Position Set Valve Position Properties Table Page 2 of 2 F 18 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Set Valve Position Sequence of Steps Solenoid 1 Close Open
158. 0 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 10 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of probe For a range of 5 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 01 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Load and Collect Properties Table Page 4 of 5 TRILUTION LH Software Version 2 0 User s Guide D 87 33 05 pue peo Load and Collect Tasks SPE D Load and Collect Properties Continued Property Name Brief Description Default Value Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 01 min Equilibration Time Load and Collect Properties Table Page 5 of 5 Load and Collect Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down
159. 1 Export a Report 1 On the Reports window click the Export button The Export Report window is displayed Select a destination folder so that it appears in the Save in box 3 Typea file name into the File name box 4 Select the file type from the Save as type box by clicking on the down arrow and then selecting the format 5 Click SAVE to save the document A message box will indicate when the export has completed TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Gilson supplies Liquid Handling tasks for use on all liquid handlers with the following exceptions GX 271 Liquid Handler with Solvent System Use SS Liquid Handling tasks see Appendix B Tasks SS Liquid Handling GX 281 Liquid Handler with Solvent System Use SS Liquid Handling tasks see Appendix B Tasks SS Liquid Handling Quad Z 215 with Disposable Tips Use Disposable Tips tasks see Appendix C Tasks Disposable Tips The Inject task can only be used on single probe liquid handlers that use an injection module The Prime 402 Dual with Tee task can only be used when the 402 Dual with Tee is the pump The Prime Transfer Ports task can only be used with the ASPEC XL4 the GX 274 ASPEC without Pump and the GX 274 Liquid Handler without Pump TRILUTION LH Software Version 2 0 User s Guide A 1 Tasks Liquid Handling Liquid Handling Liquid Handling Tasks Gilson Task Name and Description Add This t
160. 1 Rinse Stations TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Drain Rinse Station GX Extra Tall Rack Use Z Clamp Height mm Rinse Station Part Number Inside Rinse 210 26034556 Use Drain Rinse Station GX Tall Rack Z Clamp Height mm Rinse Station Part Number Inside Rinse 175 26034555 Use Drain Rinse Station GX Short Rack Z Clamp Height mm Rinse Station Part Number Drain Rinse Station for Inside Rinse of Probe Inside Rinse 125 26034554 TRILUTION LH Software Version 2 0 User s Guide G 29 suol e907 asuly Rinse Locations Rinse Locations Rinse Station GX Extra Tall Rack Z Clamp ae Height mm Rinse Station Part Number Outside Rinse 210 static or flowing 26034553 Rinse Station GX Tall Rack Z Clamp Height mm Rinse Station Part Number Outside Rinse 175 static or flowing 26034551 Rinse Station GX Short Rack Rinse Station for Rinse Station for Outside Rinse of Probe Use Z Clamp Outside Rinse of Flowing Height mm Rinse Station Part Number Probe Static Outside Rinse 125 static or flowing 26034552 G 30 TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Access the Bed Layout and Utilities by choosing Liquid Handling Utilities Bed Layout and Utilities TRILUTION LH Software Version
161. 2 0 User s Guide Bed Layout and Utilities Bed Layout and Utilities H Custom Templates Design your template with footprints You can select pre defined footprints or define a footprint of your own Information stored concerning the template includes physical dimensions and the origin where 0 0 0 is Keywords e Template The bottom required layer of a Bed Layout Only footprints can be added to templates e Origin Defined by the user the location of the coordinates X 0 Y 0 Z 0 e Footprint A placeholder or area that accepts racks e Rack A rack requires a footprint before it can be added to a template Examples of racks are Racks Rinse Stations Injection Port Bars Tip Boxes and Microplates H 2 TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Creating a Template 1 Click NEW TEMPLATE The New Template window appears Select Compatible Instruments 0215 Liquid Honedor 0215 Liquid Handler without 215 see 0215 SW Liquid Handler 0221 x Liquid Handler 0222 4 qud Handler 1223 Sample Changer The New Template Window In the New Template window enter the template dimensions 3 Select one or more compatible instrument s by checking the box Optionally click gt and then do the following a To zoom the Template toggle the Auto Scale selection off b To zoom in on the Template click once or repeatedly increments by 0 5 TRILUTION LH will zoom in to
162. 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 31 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration 4 Property Name Setup Brief Description 444 QuadDilutor ID Syringe Name Size pL Reservoir Name A 1000 Reservoir 1000 Reservoir 1000 Reservoir 1000 Reservoir The 444 QuadDilutor Properties Window Properties 444 QuadDilutor Properties Default Value Range Syringes to Use The syringes that will be used when this A B C D A B C D instrument is selected as the Pump ina Task ID A unique identifier to address syringeson A B C D A B C D the instrument 444 QuadDilutor Properties Table Page 1 of 2 4 32 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 444 QuadDilutor Properties Continued Default Property Name Brief Description Value Range Syringe Name A unique name to address syringes on the A B C D N A instrument Size uL The capacity of each installed syringe Fora 1000 100 250 500 1000 5000 10000 25000 range of suggested flow rates for this syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid Reservoi N A that is flowing through the associated r syringe It is important for sample tracking General Instrument Name The name that will be used to identify this Pump N A instrument in a Task
163. 3 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min INSIDE RINSE Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate mM A WN gt Move Z to top OUTSIDE RINSE Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Height from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate mM A U N gt Move Z top GAS AIR PURGE If SPE 215 TRUE 1 Move Z to top 2 Set Voltage On and Output Contact 1 to Close 3 Wait 0 02 min TRILUTION LH Software Version 2 0 User s Guide D 97 33 0 gt pue peo Load and Collect Tasks SPE 4 Set Voltage Off and Output Contact 1 to Open 5 Move Z to top D 98 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Move Mobile Rack This task initializes the mobile rack or moves over the drain or the collect Properties Binstruments Move Mobile Rack Collect DECZOse Name Saroe zoe For more information about the properties on each tab see D Move Mobile Rack Properties on page D 99 Collet Drain Deitialize Mobile Rack Move Mobile Rack Instruments on page D 100 Move Mob
164. 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select a Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow Rate a range of suggested flow rate values see Flow Rates on page 4 93 Derivatize Properties Table Page 2 of 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Derivatize Properties Continued A Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Solution Zone The zone from which the Solution Volume is drawn Solution Well The well in the
165. 6 OK Cancel J heip Eject Tips Properties Property Name Brief Description Default Value Tip Waste Zone The zone assigned to the tip chute at which the tips are ejected Eject Tips Properties TRILUTION LH Software Version 2 0 User s Guide C 45 sdij fJ Eject Tips Tasks Disposable Tips Eject Tips Instruments Property Name Brief Description Liquid Handler The liquid handler that the task will affect Default Value Pump The pump or VPS that the task will affect Eject Tips Instruments Eject Tips Sequence of Steps 1 Move Z to top 2 Move probe to well in Tip Waste Zone 3 Eject tips by moving Z to Z Clamp Height C 46 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Load Tips This task loads tips Properties instruments For more information about the properties on each tab see vad 60 p ON Load Tips Instruments on page C 47 Tips fe Load Tips Sequence of Steps on page C 48 Mm V on Cancel heip Load Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Load Tips Instruments TRILUTION LH Software Version 2 0 User s Guide C 47 sdi peo Load Tips Tasks Disposable Tips Load Tips Sequence of Steps 1 Move Zto top 2 If Load Tips before Task TRUE load tips Note If tips are already loaded no tips
166. 60 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dilute This task dispenses the specified volumes of liquid source from the tray and another liquid diluent from the reservoir or tray to the wells of the result zone For more information about the properties on each tab see SS Dilute Properties on page B 62 SS Dilute Advanced Inside Rinse on page B 64 SS Dilute Outside Rinse on page B 68 SS Dilute Instruments on page B 71 SS Dilute Sequence of Steps on page B 72 TRILUTION LH Software Version 2 0 User s Guide B 61 23n I0 SS SS Dilute Tasks SS Liquid Handling SS Dilute Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn 1 Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at
167. 74 Liquid Handler without Pump Properties Continued Property Name Brief Description Default Value Range Solenoid 1 Optionally type a description for the solenoid N A N A Solenoid 2 This description will be used to identify the solenoid in a Task General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task Instrument Serial Number The serial number of the instrument N A N A GX 274 Liquid Handler without Pump Properties Table Page 3 of 3 4 64 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 281 Liquid Handler The GX 281 Liquid Handler Properties Window Port ETT Not Instal Not Instal Properties GX 281 Liquid Handler Properties Property Name Brief Description Default Value Range Setup Probe Type mm This is the Z travel height 210 mm Probe 125 mm Probe 175 GX 281 Liquid Handler Properties Table Page 1 of 4 mm Probe 210 mm Probe TRILUTION LH Software Version 2 0 User s Guide 4 65 uoleinbyuo e Guljeal gt Creating a Configuration Method Builder Configuration GX 281 Liquid Handler Properties Continued Property Name Brief Description Default Value Range Z Clamp Height mm This number corresponds to where the 210 Calculated based mounting clamp is on the vertical ruler on on Z Clamp Height the installed Z arm and Probe Type Range provided
168. 97 5 26034556 33 Drain Rinse Station GX Tall Rack Use Tube Top mm Rinse Station Tube Bottom mm Part Number Inside Rinse 162 4 26034555 33 Drain Rinse Station GX Short Rack Use Tube Top mm Rinse Station Tube Bottom mm Part Number Inside Rinse 114 21 26034554 33 Drain Rinse Station GX Short 56 mm Probe Rack Use Tube Top mm Rinse Station Tube Bottom mm Part Number Inside Rinse 114 21 26034554 67 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Station for Outside Rinse of Probe Static Rinse Station for Outside Rinse of Probe Flowing TRILUTION LH Software Version 2 0 User s Guide Rinse Station GX Extra Tall Rack Tube Top mm Rinse Station use Tube Bottom mm Part Number Outside Rinse 197 5 26034553 static or flowing 33 Rinse Station GX Tall Rack Use Tube Top mm Tube Bottom mm Rinse Station Part Number Outside Rinse static or flowing 162 4 33 26034551 Rinse Station GX Short Rack Use Tube Top mm Tube Bottom mm Rinse Station Part Number Outside Rinse static or flowing 114 21 33 26034552 Rinse Station GX Short 56 mm Probe Rack Use Tube Top mm Tube Bottom mm Rinse Station Part Number Outside Rinse static or flowing 114 21 67 26034552 suo1 e gt 07 asuly Rinse Locations Rinse Locations B
169. A Output 1 This description will be used to identify the Output 2 contact in a Task Output 3 Output 4 Input A Optionally type a description for the contact N A N A Input B This description will be used to identify the Input C contact in a Task Input D General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task GSIOC ID A unique numerical address that must be 22 20 29 known to the instrument and to the software that allows the software to communicate with the instrument SPE 215 Properties Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide Creating a Configuration Method Builder Configuration VALVEMATE The VALVEMATE Properties Window Properties VALVEMATE Properties Property Name Brief Description Default Value Range Setup Valve Positions The number of ports in the VALVEMATE valve 2 2 4 6 8 Initial Valve Position The valve position after initialization at the beginning of a run 1 1 2 VALVEMATE Properties Table Page 1 of 2 4 84 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration VALVEMATE Properties Continued 4 General Instrument Name The name that will be used to identify this instrument in a Task Valve N A GSIOC ID A unique numerical address that must be known to the instrument 35 30 39 and to the software that allows the software to communic
170. Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Injection Zone The zone to which the Source Volume is dispensed Injection Well The well in the Injection Zone to which the Source Volume is delivered When using a multiple probe instrument type x where x is a value defined in the Sample List Inject Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Inject Properties Continued A Property Name Brief Description Default Value Injection Flow The speed at which the Source Volume moves out of the probe and into the 3 mL min Rate injection port Equilibration Time the program waits after dispensing to the injection port before moving out 0 min Time of the well Inject Properties Table Page 2 of 2 Inject Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height and Inject Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference
171. Air Gap at Result Flow Rate 10 Move Z to top TRILUTION LH Software Version 2 0 User s Guide peoy Load Tasks SPE Transfer Port Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time mM A WN gt Move Z to top Dispense until Source Volume delivered 6 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top 7 Move probe to well in Inside Rinse Zone 8 Lower probe to Inside Rinse Height from Tube Bottom 9 Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume not aspirated and TRILUTION LH Software Version 2 0 User s Guide Tasks SPE AIR PUSH Solenoid 1 2 3 Oo ON WD MN RA Move probe to well in Inside Rinse Zone Aspirate 250 uL at 5 mL min Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10
172. Available Instruments window select an instrument then drag and drop it in the Workspace If an instrument with the same GSIOC ID is found an option is provided to add the instrument with a new GSIOC ID TRILUTION LH Software Version 2 0 User s Guide 4 5 uoljeinbiyuo gt e Nurze Method Builder Configuration 4 Instrument Properties Every instrument has a set of properties defined in the Configuration that differ for each instrument Instrument Name For List of Properties Creating a Configuration 4 6 215 Liquid Handler See page 4 8 215 Liquid Handler without Pump See page 4 11 215 SW Liquid Handler See page 4 13 221 XL Liquid Handler See page 4 15 222 XL Liquid Handler See page 4 17 223 Sample Changer See page 4 19 402 Pump Dual See page 4 21 402 Pump Dual with Tee See page 4 23 402 Pump Single See page 4 26 406 Pump Dual See page 4 28 406 Pump Single See page 4 30 444 QuadDilutor See page 4 32 506C System Interface See page 4 34 606 GSIOC to RS 232 Converter See page 4 36 819 Injection Module See page 4 39 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Instrument Name For List of Properties ASPEC XL See page 4 40 ASPEC XL4 See page 4 42 ASPEC XLi See page 4 45 GX 271 ASPEC without Pump See page 4 47 GX 271 Liquid Han
173. Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Syringe 1 2 Move probe to DEC Well in DEC Zone Move Z to top TRILUTION LH Software Version 2 0 User s Guide 33 0 gt pue peo Load and Collect Tasks SPE CON WDM HR W 9 10 11 12 Val CON AO MN BP WN c 9 Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top Dispense Air Push Volume at Dispense Flow Rate Wait Equilibration Time Move Z to top performing shake off SPE 215 Repeat steps 4 9 until Air Push Volume is dispensed Move probe to Inside Rinse Wells at Inside Rinse Zone Dispense Air Gap at Dispense Flow Rate ve Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D 12 Wait Equilibration Time 1
174. D 5 Condition Advanced Rinsing on page D 9 Condition Instruments on page D 11 Condition Sequence of Steps on page D 12 D 4 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Condition Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into the probe For a range of 6 mL min Source Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Condition Properties Table Page 1 of 5 TRILUTION LH Software Version 2 0 User s Guide D 5 UO puo gt Condition Tasks SPE D Condition Properties Continued Property Name Brief Description Default Value Tray Th
175. Default Value GX 274 The speed at which the Volume moves into the probe For a range of suggested 10 mL min Aspirate Flow flow rate values see Flow Rates on page 4 93 Rate GX 274 The speed at which the Volume moves out of the probe For a range of suggested 10 mL min Dispense Flow flow rate values see Flow Rates on page 4 93 Rate GX 274 The quantity of the air gap aspirated before the Volume 20 uL Air Gap GX 274 The speed at which the Air Gap moves into the probe 0 3 mL min Air Gap Flow Rate Prime Transfer Ports Properties Table Page 3 of 3 Prime Transfer Ports Rinsing Property Name Brief Description Default Value Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe Prime Transfer Ports Rinsing Table Page 1 of 2 250 uL A 100 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Prime Transfer Ports Rinsing Continued A Property Name Brief Description Default Value Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outsi
176. GSIOC ID A unique numerical address that must be known to the instrument and to the software that allows the software to communicate with the instrument 215 Liquid Handler Properties Table Page 3 of 3 22 20 29 4 10 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 215 Liquid Handler without Pump The 215 Liquid Handler without Pump Properties Window Properties 215 Liquid Handler without Pump Properties Property Name Brief Description Default Value Range Setup Z Arm Type mm This is the height of the Z arm installed on the 125 mm Z Arm 125mm Z Arm liquid handler 175 mm Z Arm Z Clamp Height mm This number corresponds to where the mounting 125 0 175 Le is on the vertical ruler on the installed Range provided i on screen 215 Liquid Handler without Pump Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 11 uo eInp zuo e Nurze Creating a Configuration Method Builder Configuration 215 Liquid Handler without Pump Properties Continued Property Name Brief Description Default Value Range Z Safe Height mm The Z height to which the instrument s probe 122 Calculated based on moves before moving in the X or Y direction Z Clamp Height and Z Arm Type Range provided on screen Contacts 24V Output Optionally type a description for the contact This N A N A Output 1 description
177. ID selected The Comment field is not used Selecting the Buffered button will not add the command to the command list For more information see Send a Buffered Command on page K 25 TRILUTION LH Software Version 2 0 User s Guide AINN DOISD GSIOC Utility GSIOC Utility Response field Returns a response to an immediate or buffered command The response will become gray after 15 seconds if no response is registered The response to a successfully completed buffered command is ok Refer to the user s guide for the Gilson instrument for a description of the valid response to the immediate commands The response to an unsuccessfully completed immediate or buffered command is error To send a command line Double click on the Unit Type Command Response or Comment field to send the immediate or buffered command for that specific command line TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility Command line column headings Repeat A green check mark in the Repeat column means that an immediate command will automatically repeat when the Auto Repeat option is checked Double click on the Repeat field to select or deselect the Auto Repeat for that command line A yellow check mark 7 in the Repeat column means that a buffered command will automatically repeat when the Auto Repeat option is checked Double click on the Repeat field to select or deselect the Auto Repeat option for t
178. ION LH Software Version 2 0 User s Guide GSIOC Utility Listing GSIOC Instruments Using the GSIOC Utility you can determine the instruments currently connected to the computer In the Mode menu select Scan The Unit ID list box displays the unit IDs and the version of the connected instruments If any connected instruments are missing from the list ensure that the proper RS 232 or GSIOC connection exists between the computer and the instruments and that the instruments do not have duplicate unit ID numbers TRILUTION LH Software Version 2 0 User s Guide K 3 AINN DOISD GSIOC Utility GSIOC Utility K 4 Basic Mode In the Basic mode immediate and buffered GSIOC commands can be sent to specific Gilson instruments Review the Port IRQ and Baud information in this window If any information is incorrect or missing close the GSIOC Utility and use the GSIOC Configuration Editor to update the information There are two drop down menus in the Basic mode of the Gilson GSIOC Utility Mode Menu and Help Menu TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility K Basic Mode Buttons and Features Immediate button Sends an immediate command to the Unit ID selected Immediate commands request status information from an instrument These commands are executed immediately temporarily interrupting any command in progress Each command is a string of no more than 40 characters You can find a list
179. L Prewet Tip has been selected Touch Off Mode Used when Touch Off was selected on the Properties tab select Side or Surface Surface touch off Cherry Pick with Tips Tips Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide C 7 sd YIM 991d A1149 Cherry Pick with Tips Tasks Disposable Tips Cherry Pick with Tips Tips Continued Property Name Brief Description Default Value Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Surface number is entered from an auto calculated position to which the tip will move Z Offset when performing a surface Touch Off Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Touch Off The distance right when a positive number is entered or left when a negative 0 mm Mode Side number is entered from the edge of the tube that the tip will move when X Offset performing a side Touch Off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch Off Select Side Surface or None no rinse touch off performed None Mode Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative num
180. LUTION LH Software Version 2 0 User s Guide Application Importing a Sample List 1 On the Application window click the Sample List Import IF button The Select Sample List Export Files window is displayed Select Sample List Export Files 2 x Look in E Working z ec My Recent Documents E Desktop 2 My Documents bo n My Computer My Network File name JExample ts Places Files of type Sample List File tsl gt Cancel A The Select Sample List Export Files window 2 On the Select Sample List Export Files window do the following a Browse for and select the Sample List file b Click OPEN Make certain that your Sample List matches your Application same Configuration and Bed Layout TRILUTION LH Software Version 2 0 User s Guide s17 a dwes e Hur rodwj Run Results Window Application Run Results Window Results are accessed by clicking RUN RESULTS in the Utilities menu in the Liquid Handling menu or by clicking RESULTS in the Application window Run Results Run Results Window To sort the run results click on a column header To sort in reverse order click the column header again TRILUTION LH Software Version 2 0 User s Guide Application 8 The following action buttons are located in the lower left corner of the D Run Results window 5 J M Action Button Description c e VIEW LOG Opens the log file detailing information
181. Liquid Level Detection is used A low 1 setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Result Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Aliquot Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A A
182. List Result Flow Rate The speed at which the reservoir solvent moves into the syringe and the probe 5 mL min and into the Result Wells For a range of suggested flow rate values see Flow Rates on page 4 93 Number of Enter the number of times to repeat aspirating and dispensing 2 Cycles Prime 402 Dual with Tee Properties Prime 402 Dual with Tee Advanced Rinsing Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2 mm number is entered from the tube bottom when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Prime 402 Dual with Tee Advanced Rinsing Table Page 1 of 2 Outside Rinse TRILUTION LH Software Version 2 0 User s Guide L YUM Jend ZOp awd Prime 402 Dual with Tee Tasks Liquid Handling Prime 402 Dual with Tee Advanced Rinsing Continued A Property Name Brief Description Default Value Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse
183. MOVE X Y Y Enter a number to move to a position forward of the home 0 0 mm position 215 Liquid Handler Manual Control Table Page 1 of 3 9 4 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 215 Liquid Handler Manual Control Continued Property Name Brief Description Default Value Use Z Enter a number to move to an absolute Z value Click MOVE Z Output Contacts 24V A filled box indicates that the circuit is closed on An empty Toggle the contacts 1 box indicates that the circuit is open off open closed off 2 on by selecting 3 and clearing the 4 boxes Input Contacts A A filled box indicates that the circuit is closed An empty box Monitor status B indicates that the circuit is open C D Pump Prime Flow Rate The speed at which the reservoir fluid moves into and out of 10 mL min Click PRIME to start the syringe Or STOP PRIME to end Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration 215 Liquid Handler Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 5 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Manual Control 9 Default Value Use Used when replacing a syringe Application 215 Liquid Handler Manual Control Continu
184. Mix with Liquid Mix with with Liquid Liquid Mix with Liquid The quantity of liquid used as part of a mixing process OuL Mix Volume Mix with Liquid The quantity of air gap aspirated before the Mix Volume 20 uL Air Gap Mix with Liquid The speed at which the Mix Volume moves into and out of the probe Fora range 5 mL min Flow Rate of suggested flow rate values see Flow Rates on page 4 93 Mix with Liquid The speed at which the Air Gap moves into the probe For a range of suggested 0 3 mL min Air Gap Flow flow rate values see Flow Rates on page 4 93 Rate Mix with Liquid Time the program waits after each dispense before moving out of the well 0 min Equilibration Time Mix Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide A 81 XIW Mix Tasks Liquid Handling Mix Properties Continued A Property Name Brief Description Default Value Mix with Liquid Select Touch Off to move to the edge of the well after dispensing in the X Touch Off direction Mix with Air The quantity of air used as part of a mixing process O uL Mix Volume Mix with Air The quantity of air gap aspirated before the Mix Volume 20 uL Air Gap Mix with Air The speed at which the Mix Volume moves into and out of the probe For a range 5 mL min Flow Rate of suggested flow rate values see Flow Rates on page 4 93 Mix with Air Time the program waits after each dispense before moving out of th
185. Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 7 If Source Volume is greater than tip volume repeat aspirate and dispense until Source Volume is delivered nn A U N gt if Extra Volume gt 0 1 Move probe to Result Well in Result Zone 2 Lower probe into well to Result Height 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected 4 Wait Equilibration Time TRILUTION LH Software Version 2 0 User s Guide C 87 sdi YUM JajsuelL Transfer with Tips Tasks Disposable Tips 5 If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 6 If Source Volume is greater than tip volume repeat aspirate Air Gap and Extra Volume are not aspirated and dispense until Source Volume is delivered 7 Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height 9 Dispense Extra Volume Source Air Gap at Result Flow Rate EJECT TIPS OPTIONAL If Eject Tips After Task TRUE 1 Move Z to top 2 Move probe to Tip Waste Zone 3 Eject tips by moving Z to Z Clamp Height C 88 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips INSIDE RINSE OPTIONAL Onn Bp WN gt Move Z to top Move
186. Name Brief Description Default Value Use Click PRIME to start or STOP PRIME to end Prime Flow Rate 10 mL min The speed at which the reservoir fluid moves into and out of the syringes Buttons HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 402 Pump Dual with Tee Manual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 9 21 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Manual Control 9 Default Value Use Used when replacing a syringe Application 402 Pump Dual with Tee Manual Control Continued Property Name Brief Description LOWER SYRINGE Descends the syringes piston operating rods 25 of a full stroke as the syringe pump aspirates from the reservoir The valve will switch to the outlet position RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 402 Pump Dual with Tee Manual Control Table Page 2 of 2 9 22 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 402 Pump Single 402 Pump Singe Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 402 Pump Single Manual Control Property Name Brief Description Default Value Use
187. Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Active Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Source Height The distance up when a positive number is entered or down when a negative 2 mm number is entered from a defined reference point Tube Bottom unless Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No rinse is performed O uL when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe No rinse is performed
188. Number Active Text Inactive Text Color T Color Active Zone j Inactive Zone Color Color T OK Cancel Active Sample The Zone Properties Window Active Text Color The color that is used for the numbers of the wells when the zone is active Inactive Text Color The color that is used for the numbers of the wells when the zone is inactive TRILUTION LH Software Version 2 0 User s Guide Method Builder Bed Layout Active Zone Color The color used for the wells in the zone when it is active As new zones are added the Active Zone Color automatically increments The zone becomes active when it is selected from the Zone drop down menu Only one zone can be active at a time Refer to the Sample in the dialog to see the Active Text Color on the Active Zone Color e Inactive Zone Color The color used for the wells in the zone when it is inactive The zone becomes inactive when another zone is selected from the Zone drop down menu Multiple zones can be inactive at one time 3 Click OK Modifying Zones To modify a zone 1 Double click Zone Name from the Zone Management list 2 Make changes to the name starting number and or colors 3 Click OK Deleting Zones To delete a zone select the Zone Name in the Zone Management list and then click DELETE TRILUTION LH Software Version 2 0 User s Guide 5 5 MOPUIM qe 3NoAe7 pag Bed Layout Tab Window Method Builder Bed Layout 5 5 6 Number
189. OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into and out ofthe 3 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Fractionate Properties Table Page 2 of 5 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Fractionate Properties Continued Property Name Brief Description Default Value DEC Zone The zone to which the Source Volume is delivered DEC Well The well in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Collection Zone The zone where the analytes of interest will be collected Collection Well The well where the analyte of interest will be collected When using a multiple 1 probe instrument type x where x is a value defined in the Sample List
190. PE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Syringe 1 2 Move probe to DEC Well in DEC Zone Move Z to top TRILUTION LH Software Version 2 0 User s Guide 9 euU01 e114 Fractionate Tasks SPE CON WDM HR W 9 Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top Dispense Air Push Volume at Dispense Flow Rate Wait Equilibration Time Move Z to top performing shake off SPE 215 10 Repeat steps 4 9 until Air Push Volume is dispensed 11 Move probe to Inside Rinse Wells at Inside Rinse Zone 12 Dispense Air Gap at Dispense Flow Rate Valve CON AO MN BP WN c 9 Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move
191. Powers all motors and moves the probe to the home position HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position ASPEC XL4 Manual Control Table Page 3 of 4 9 38 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control ASPEC XL4 Manual Control Continued Property Name Brief Description LOWER SYRINGE Descends the syringe s piston operating rod 25 of a full stroke as the syringe pump aspirates from the reservoir The valve will switch to the outlet position Default Value Use RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position ASPEC XL4 Manual Control Table Page 4 of 4 Used when replacing a syringe TRILUTION LH Software Version 2 0 User s Guide 9 39 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control Property ASPEC XLi ASPEC XLi Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons ASPEC XL4 Manual Control Mae Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up whena positive number 2mm Click MOVE TO WELL is entered or down when a negative number is entered fr
192. Property Name Brief Description Value Range Setup ID A unique identifier to address syringes on the A A instrument 402 Pump Dual with Tee Properties Table Page 1 of 2 4 24 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 402 Pump Dual with Tee Properties Continued ae Default Property Name Brief Description Value Range Syringe Name A unique name to address syringes on the A N A instrument Size uL The capacity of the left syringe A For a range of 10000 100 250 500 1000 5000 suggested flow rates for this syringe size see Flow 10000 25000 Rates on page 4 93 Reservoir Name A unique name used to identify the liquid thatis Reservoi N A flowing through the associated syringe It is r important for sample tracking Right Syringe Size uL The capacity of the right syringe B For a range of 500 100 250 500 1000 5000 suggested flow rates for this syringe size see Flow 10000 25000 Rates on page 4 93 General Instrument Name The name that will be used to identify this Pump N A instrument in a Task GSIOC ID A unique numerical address that must be known 0 0 31 to the instrument and to the software that allows the software to communicate to the instrument 402 Pump Dual with Tee Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 25 uoleinbiyuo e Guljeal gt Creating a Configuration Method Builde
193. Quad Z 215 Properties Window Properties Quad Z 215 Properties Property Name Brief Description Default Value Range Setup Z Clamp Height mm This number corresponds to where the 175 0 190 mounting clamp is on the vertical ruler on the Range provided installed Z arm Bn TEA Quad Z 215 Properties Table Page 1 of 3 4 78 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Quad Z 215 Properties Continued Property Name Brief Description Default Value Range Z Safe Height mm The Z height at which the instrument s probe 172 Calculated based on moves to before moving in the X or Y direction Z Clamp Height and For Quad Z 215 with Tips TRILUTION LH considers Z S Range the installed tip when determining the Z Safe P A ERE Height Probes to Use The probes that will be used when this A B C D A B C D instrument is selected as the Pump in a Task ID A unique identifier to address probes on the A B C D A B C D instrument Tip Type The tips installed on the liquid handler None DL10 Tip Holder Type A When a tip type is selected a tip zone is o a B automatically created in the Bed Layout l pe Contacts 24V Output Optionally type a description for the contact N A N A Output 1 This description will be used to identify the Output 2 contact in a Task Output 3 Output 4 Quad Z 215 Properties Table Page 2 of 3 TRILUTION LH Software Version
194. RILUTION LH Software Version 2 0 User s Guide B 27 onbilY SS SS Aliquot Tasks SS Liquid Handling SS Aliquot Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Aliquot Outside Rinse Table Page 3 of 4 B 28 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Aliquot Outside Rinse Con
195. Range Port 1 Optionally type a description of the N A N A Port 2 solvent valve port Port 3 Port 4 Port 5 Port 6 Contacts Output 1 Optionally type a description for N A N A Output 2 the contact This description will be 24V Output 1 used to identify the contact in a 24V Output 2 Task Additionally indicate whether the contact should open close or remain in the same state on error The default is No Change for Outputs 1 and 2 and Open for 24V Outputs 1 and 2 Input A Optionally type a description for N A N A Input B the contact This description will be used to identify the contact in a Task GX 271 Liquid Handler Properties Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide 4 53 uoleinbyuos e Gules Creating a Configuration Method Builder Configuration GX 271 Liquid Handler Properties Continued Property Name Brief Description Default Value Range Solenoid 1 Optionally type a description for N A N A Solenoid 2 the solenoid This description will be used to identify the solenoid in a Task General Instrument Name The name that will be used to Liquid Handler N A identify this instrument in a Task Instrument Serial Number The serial number of the N A N A instrument Pump Serial Number The serial number of the solvent N A N A system Injection Module Serial Number The serial number of the injection N A N A module GX 271 Liquid Handler Prop
196. Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 3 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 1 min Time Reset Mobile The reset mobile rack moves the mobile rack over the drain Rack Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Fractionate Properties Table Page 3 of 5 TRILUTION LH Software Version 2 0 User s Guide D 55 9 euU01 3e14 Fractionate Tasks SPE D Fractionate Properties Continued Property Name Brief Description Default Value Solenoid Time the program waits after a solenoid air push before moving out of the well 0 1 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 20 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 6 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of the probe Fora range of 6 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate
197. Rinse Table Page 1 of 4 B 106 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Inject Advanced Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the O
198. Software Version 2 0 User s Guide 10 Working with Variables 11 Generating Reports Variables The Reports Window Creating a New Variable Reports Overview Task Report Application Report Run Report Method Report Sample Tracking Report View a Report Viewing Toolbar Export a Report TRILUTION LH Software Version 2 0 User s Guide A Tasks Liquid Handling B Tasks SS Liquid Handling Aliquot SS Aliquot Cherry Pick SS Cherry Pick Derivatize SS Derivatize Dilute SS Dilute Dispense SS Dispense Dispense Random to Random SS Dispense Random to Random SS Prime Pump Prime Pump SS Rinse Probes Prime Transfer Ports SS Transfer Rinse Probes Transfer TRILUTION LH Software Version 2 0 User s Guide C Tasks Disposable Tips D Tasks SPE Cherry Pick with Tips Dispense Random to Random with Tips Dispense with Tips Evaporate Eject Tips Fractionate Load Tips Mix with Tips Prime Pump with Tips Prime Rinse Station Rinse Tip Holders Transfer with Tips TRILUTION LH Software Version 2 0 User s Guide E Tasks Tweaks F Tasks Auxiliary Aspirate Aspirate with Tips Deliver Run Executable Deliver with Tips Scan Bar Codes Move To Set Electrical Contact Move To Zone Set Valve Position SS Aspirate SS Deliver TRILUTION LH Software Version 2 0 User s Guide G Rinse Locations H Bed Layout and Utilities 215 Family Custom Templates 221 XL Liquid Handler Creating a Template Deleting a Template 222 XL Liqu
199. Solution Volumes are delivered Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered When using a multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source and Solution Volumes move out of the probe For 5 mL min a range of suggested flow rate values see Flow Rates on page 4 93 Add Properties Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Add Properties Continued A Property Name Brief Description Default Value Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Add Properties Table Page 4 of 4 Add Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height Solution Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height Solution Height and Result Heigh
200. T Select the Rack List or Template List option button Select a Rack or Template Click L The Browse For Folder window is displayed On the Browse For Folder window select a folder to which the Rack or Template will be exported and then click OK U A WN gt 6 Click oK On completion of the export operation the Rack or Template is saved in the specified folder with a LHR Rack or LHT Template extension The TRILUTION information dialog box is displayed a On the TRILUTION information dialog box do the following 1 To view the log information of the export operation click DETAILS 2 Click OK H 20 TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Importing Racks and Templates 1 Click IMPORT The Select Rack Template Files window is displayed 2 From the Select Rack Template Files window do the following a Browse for and select the Rack or Template Exported Files To select multiple Racks or Templates in a sequential order hold SHIFT any arrow key simultaneously To select Racks or Templates in a random order hold CTRL and select multiple Racks or Templates with the mouse b Click OPEN On completion of the Import operation the TRILUTION information box is displayed c On the TRILUTION information dialog box do the following 1 To view the log information of the import operation click DETAILS 2 Click OK TRILUTION LH Software Version 2 0 Use
201. TION LH Software Version 2 0 User s Guide B 57 9ZILALIBG SS SS Derivatize Tasks SS Liquid Handling MIX Liquid 1 Move probe to Result Well in Result Zone same as in DISPENSE 2 Aspirate Mix Air Gap Volume at Mixing Flow Rate 3 Lower probe into well to Result Height 4 Aspirate Mixing Volume at Mixing Flow Rate using Liquid Level Following if selected 5 Lower probe into well to Result Height 6 Switch solvent valve to Solvent Valve Position 7 Dispense Mixing Volume at Mixing Flow Rate using Liquid Level Following if selected 8 Wait Equilibration time 9 Repeat steps 3 8 until Number of Mixes completes 10 Perform a Touch Off X direction if selected and move Z to Z Safe Height 11 Move probe to Inside Rinse Well in Inside Rinse Zone 12 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 13 Dispense Mix Air Gap Volume at Mixing Flow Rate Air Nun A U Nc B 58 Move probe to Result Well in Result Zone same as in Dispense Aspirate Mix Air Gap Volume at Mixing Flow Rate Move Z to Z Safe Height Aspirate Mixing Volume at Mixing Flow Rate Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B 7 Dispense Mixing volume at Mixing Flow Rate 8 Wait Equilibration Time 9 Repeat steps 3 8 until Number of Mixes completes 10 Perform a Touch Off X direction if selected and move Z
202. TION LH will remember your user name Change Password A member of the default Admin group provides the user name and the default password To change the Password 1 On the main menu click ADMINISTRATIVE TOOLS 2 Onthe Administrative Tools menu select the CHANGE PASSWORD option The Change Password window displays TRILUTION LH Software Version 2 0 User s Guide 1 19 snual H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 1 20 3 On the Change Password window do the following a In the Current User Name field verify your user name b In the Current Password field type your old password c In the New Password field type a new password d In the Confirm Password field type the new password again e Click OK The password is changed Ji Password is case sensitive and has a 20 character limit Users and Groups A User is an end user of the product Every User is assigned to a Group based on the roles A Group includes Users with common rights The Users and Groups menu allows you to create modify delete and view Users and Groups During the TRILUTION LH installation by default two groups are created Admin and Analyst Administrator is the default user in the Admin group The Admin group is assigned all the rights Analyst is the default user in the Analyst group The Analyst group can view create and modify in TRILUTION LH TRILUTION LH Software Version 2 0 User
203. TRILUTION LH v2 0 User s Guide TRILUTION LH v2 0 User s Guide Gilson Inc World Headquarters 3000 Parmenter Street P O Box 620027 Middleton WI 53562 0027 USA Telephone 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 www gilson com sales gilson com service gilson com training gilson com 2006 Gilson Inc All rights reserved Table of Contents About the User s Guide 1 TRILUTION LH Software Overview Unpacking TRILUTION LH Glossary Customer Service Before Calling Us Training Quick Start Tutorial Introductory TRILUTION LH Training 4TRTRILUTION1 2 days Advanced TRILUTION LH Training TRTRILUTION2 2 days Launching TRILUTION LH Windows 2000 Windows XP TRILUTION LH Menus Liquid Handling Menu Administrative Tools TRILUTION LH Software Version 2 0 User s Guide 2 Task Builder 3 Method Builder Task Builder Method Builder Tasks Palette Action Buttons Operators Palette Configuration Tab Commands Palette Bed Layout Tab Workspace Method Tab Action Buttons Error Handling Tab Creating a Task Viewing a Method Viewing a Task Saving a Method Modifying a Task Exporting Methods Saving a Task Importing Methods Exporting Tasks Deleting Methods Importing Tasks Closing the Method Builder Deleting Tasks Closing the Task Builder Close Open Tasks Close All Open Tasks Close the Task Builder TRILUTION LH Software Version 2 0 User s Guide 4 Method Bu
204. Tasks SPE CON WN HR W 9 Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top Dispense Air Push Volume at Dispense Flow Rate Wait Equilibration Time Move Z to top performing shake off SPE 215 10 Repeat steps 4 9 until Air Push Volume is dispensed 11 Move probe to Inside Rinse Wells at Inside Rinse Zone 12 Dispense Air Gap at Dispense Flow Rate Valve CON AO MN BP WN 9 Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe TRILUTION LH Software Version 2 0 User s Guide a ye1odeny Evaporate Tasks SPE 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min INSIDE RINSE Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate mM A WN gt Move Z to top OUTSIDE RINSE Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Heig
205. The capacity of each installed syringe Fora 1000 500 1000 2500 5000 range of suggested flow rates for this syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid Reservoir N A that is flowing through the associated syringe It is important for sample tracking Multiple Probe 215 Properties Table Page 2 of 3 4 76 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Multiple Probe 215 Properties Continued Property Name Brief Description Default Value Range uoleinbyuo e buljeal gt D Contacts 24V Output Optionally type a description for the contact N A N A Output 1 This description will be used to identify the Output 2 contact in a Task Output 3 Output 4 Input A Optionally type a description for the contact N A N A Input B This description will be used to identify the Input C contact in a Task Input D General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task GSIOC ID A unique numerical address that must be 22 20 29 known to the instrument and to the software that allows the software to communicate with the instrument Multiple Probe 215 Properties Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide Creating a Configuration Method Builder Configuration Quad Z 215 TipType None None None None The
206. The following is an overview for the sequence of steps 1 Rinse Touch Off on page C 70 RINSE TOUCH OFF Side Move Z to top Move probe to well in Inside Rinse Zone Move probe to Side Rinse Z Offset Move probe 1 5 mm in the Y direction forward Move probe to Side Rinse Y Offset Move probe to the center of the well N OAU A WU N gt Move Z to top C 70 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Surface 1 Move Z to top 2 Move probe to well in Outside Rinse Zone 3 Move probe to Surface Rinse Z Offset 4 Move Ztotop None 1 Move Z to top TRILUTION LH Software Version 2 0 User s Guide UOI L S BSUIY BUI Rinse Tip Holders Tasks Disposable Tips C Rinse Tip Holders This task rinses the inside and outside of the tip holders For more information about the properties on each tab see Rinse Tip Holders Properties on page C 73 Rinse Tip Holders Instruments on page C 74 Rinse Tip Holders Sequence of Steps on page C 75 C 72 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Property Name Inside Rinse Zone Rinse Tip Holders Properties Brief Description The zone to which the Inside Volume is delivered Default Value Inside Rinse Outside Rinse Zone The zone to which the Outside Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No
207. The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Dispense Advanced Rinsing Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide su ds q Dispense Tasks Liquid Handling A Dispense Instruments Property Name Description Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that
208. UE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 Shake Off FALSE a Move Z to top Move probe to well in Inside Rinse Zone Lower probe unto wells at Inside Rinse Height from Tube Bottom Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top D 38 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Transfer Port mM A U N gt 7 8 9 Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume not aspirated and Dispense until Source Volume delivered If SPE 215 Shake Off TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 Shake Off FALSE a Move Z to top Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top AIR PUSH Solenoid 1 2 Move probe to well in Inside Rinse Zone Aspirate 250 uL at 5 mL min TRILUTION LH Software Version 2 0 User s Guide 3 n J Elute Tasks SPE D 3 Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Volt
209. User s Guide E 15 Sdi YUM AaAljaq Move To Tasks Tweaks Move To This task moves the probe to the top home specified XY Coordinates or specified Z Coordinate For more information about the properties on each tab see Properties Binstruments l le Move To Properties on page E 16 Mow To Move To Instruments on page E 17 aad Too Home XY Coordinetes I 2Coordenste Move To Sequence of Steps on page E 18 Move To Properties Property Name Brief Description Default Value Move To Select one of the locations to move to Top Home XY Coordinates or Z Top Coordinates XY Coordinate X coordinate to move to 0 mm X Coordinate Move To Properties Table Page 1 of 2 E 16 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Move To Properties Continued Property Name Brief Description Default Value XY Coordinate Y coordinate to move to 0 mm Y Coordinate Z Coordinate Z coordinate to move to 0 mm Z Coordinate Move To Properties Table Page 2 of 2 Move To Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Move To Instruments TRILUTION LH Software Version 2 0 User s Guide E 17 OL AOW Move To Tasks Tweaks Move To Sequence of Steps If Top TRUE e Move Z to top If Home TRUE e Move probe to Home If XY Coordinates TRUE e Move
210. User s Guide H 7 s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities H Creating a Footprint 1 Right click the template and select Add Elements The Template Elements dialog appears In the Template Elements dialog click NEW 3 Enter a Footprint Name for the footprint 4 Select the Shape Rectangle or Circle a For a rectangle enter the Length and Width b Fora circle enter the Diameter 5 Click OK OO new Footprint rootorit Name The New Footprint Window H 8 TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Deleting a Template 1 Click DELETE TEMPLATE The Delete Template window displays Delete lemplate Mama Verson Desonption jiki 7 Position for Autom 215 SW 215 for Code 517 Rack 215 for Five Code 200 5 215 for Seven XL Racks 223 for Antlers Code 223 for Antlers Code 223 for Four Code 20 5 223 for Four Code 20 5 223 for Four Deep Micro 223 for Four Shallow Mic ASPEC XL ASPEC XL4 Narrow Racks ACNES Vi A Ahri D eke The Delete Template Window 2 Select the template to be deleted and click DELETE If more than one version of the template exists TRILUTION LH will confirm that you want to delete all versions of the template TRILUTION LH Software Version 2 0 User s Guide s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities Custom Racks Create a rack by specifying the dimensions for the ra
211. User s Guide Application 8 Exporting a Run To export a Run 1 On the Run Results window do one of the following e Select the run name and then right click and select EXPORT RESULT Select the run name and then click EXPORT To select multiple run results in a sequential order hold SHIFT any arrow key simultaneously To select run results in a random order hold CTRL and select multiple run results with the mouse 2 Select a folder and click SAVE On completion of the Export operation the Run is saved in the specified folder with a LHRE extension The TRILUTION information dialog box is displayed 3 On the TRILUTION information dialog box do the following To view the log information of the Export operation click DETAILS e Click OK TRILUTION LH Software Version 2 0 User s Guide 8 23 MopulM s Nsay UNy Run Results Window Application 8 24 Archiving a Run You will have to archive run data before the Application can be deleted To archive a Run 1 On the Run Results window select the run name and then do one of the following a Click ARCHIVE b Right click and select ARCHIVE RESULT To select multiple run results in a sequential order hold SHIFT any arrow key simultaneously To select run results in a random order hold CTRL and select multiple run results with the mouse The difference between Export and Archive is that Export leaves data in the Application Run Result
212. Value Tip Waste Zone The zone assigned to the tip chute at which the tips are ejected Load Tips Before Select and tips will be loaded at the beginning of the task Active Task Clear and tips will not be loaded If tips were previously loaded by another task clear this option Eject Tips After Select and all tips will be ejected before the rinses Active Task Clear and the tips will remain loaded Prewet Tip Select this option to wet the tip before aspirating the Source Volume 1st Iteration Cleared only Prewet Volume The quantity of liquid source aspirated and dispensed to prewet the tip when 10 uL Prewet Tip has been selected Touch Off Mode Used when Touch Off was selected on the Properties tab select Side or Surface Surface touch off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Surface number is entered from an auto calculated position to which the tip will move Z Offset when performing a surface Touch Off Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Mix with Tips Tips Table Page 1 of 2 C 52 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Mix with Tips Tips Continued Property Name Brief Description Default Value Touch Off The distance right when a positive number is e
213. Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Move probe to Diluent Well in Diluent Zone CON OD WM Aspirate Diluent Air Gap at Air Gap Flow Rate 9 Lower probe into well to Diluent Height 10 Aspirate Diluent Volume at Diluent Flow Rate using Liquid Level Following if selected 11 Move Z to Z Safe Height Transfer Port Move Z to Z Safe Height Move probe to Source Well in Source Zone Aspirate Source Air Gap at Air Gap Flow Rate Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Move probe into well in Diluent Zone CON OW PWN c Aspirate Diluent Air Gap at Air Gap Flow Rate 9 Lower probe into well to Diluent Height 10 Set Transfer Port to On 11 Aspirate Diluent Volume at Diluent Flow Rate TRILUTION LH Software Version 2 0 User s Guide 93niid Dilute Tasks Liquid Handling A 12 Set Transfer Port to Off 13 Move Z to Z Safe Height DISPENSE Reservoir 1 Move probe to Result Well in Result Zone Lower probe into well to Result Height Dispense to Source Volume Source Air Gap Diluent Volume from Reservoir at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Tray or Transfer port Move probe to Result Well in Result Zone Lower probe i
214. Volume for the well Maximum Volume must be a number other than zero 0 Click OK Creating a Target Nn A U Nc 7 Right click on a rack and then select ADD ELEMENTS Click Well and then click NEW or EDIT Right click in the Targets grid and select ADD Enter a Target Name for the target Select a Target Type from the drop down list Enter the X Y and Z Relative positions for the target The position is calculated with respect to the center of the well Click OK to exit the New Target dialog Modifying a Target Right click on a rack and then select ADD ELEMENTS Select the Well that contains the Target and then click EDIT The Well Properties dialog is displayed Right click on the Target and select EDIT Change the Target Name for the target if desired TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities 5 Change the relative X Y and Z positions for the target if desired The position is calculated with respect to the center of the well 6 Click OK to exit the Target Properties dialog Deleting a Target 1 Right click on a rack and then select ADD ELEMENTS 2 Select the Well that contains the Target and then click EDIT The Well Properties dialog is displayed 3 Right click on the Target and select DELETE 4 Click OK to exit the Target Properties dialog Editing a Custom Element You can perform the following editing actions Viewing Elements 1 To view the element
215. Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Transfer Advanced Inside Rinse Table Page 3 of 3 Omm B 150 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Transfer Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the outside rinse position in the rinse station SS Transfer Outside Rinse Table Page 1 of 4 10 mL min TRILUTION LH Software Version 2 0 User s Guide B 151 JBJSUCAL SS SS Transfer Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Transfer Outside Rinse Continued
216. Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position MB WN gt Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off 7 Move Z to Z Safe Height Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station TRILUTION LH Software Version 2 0 User s Guide B 125 XIW SS SS Prime Pump Tasks SS Liquid Handling B SS Prime Pump This task primes the transfer tubing with reservoir solvent in the result zone For more information about the properties on each tab see SS Prime Pump Properties on page B 127 SS Prime Pump Advanced Inside Rinse on page B 127 SS Prime Pump Outside Rinse on page B 130 SS Prime Pump Instruments on page B 133 SS Prime Pump Sequence of Steps on page B 134 B 126 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Prime Pump Properties Property Name Brief Description Default Value Source Source for Prime Pump is Reservoir Reservoir Solvent Valve Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Position position designated for waste cannot be
217. Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Switch Injection Valve Position to LOAD N WO UW A U N gt DISPENSE Move probe to Injection Well in Injection Zone Lower probe into well to Inject Height from Tube Bottom Switch solvent valve to Solvent Valve Position Dispense Source Volume at Injection Flow Rate U A U N gt Wait Equilibration Time B 110 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B Switch Injection Valve Position to INJECT Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset ON O 0 Dispense Source Air Gap Extra Volume at Result Flow Rate INSIDE RINSE Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Switch solvent valve to Inside Rinse Solvent Valve Position MB WN gt Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE Move probe to first
218. Zone and mixing Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Derivatize Advanced Inside Rinse Table Page 2 of 3 B 50 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Derivatize Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negati
219. a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate Load and Collect values see Flow Rates on page 4 93 Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 33 05 pue peo Load and Collect Tasks SPE D Load and Collect Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse
220. acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Injection Zone The zone to which the Source Volume is dispensed Injection Well The well in the Injection Zone to which the Source Volume is delivered 1 Injection Valve The Injection Valve to use for the task Select 2 for the Left Direct Inject and 3 for 2 ID the Right Direct Inject SS Inject Properties Table Page 1 of 2 B 102 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Inject Properties Continued Property Name Brief Description Default Value Injection Flow The speed at which the Source Volume moves out of the probe and into the 3 mL min Rate injection port Equilibration Time the program waits after each dispense before moving out of the well 0 min Time SS Inject Properties Table Page 2 of 2 SS Inject Advanced Inside Rinse Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as i
221. age 4 93 Mix with Liquid The speed at which the Air Gap Volume moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Mix with Tips Properties Table Page 1 of 2 C 50 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Mix with Tips Properties Continued Property Name Brief Description Default Value Mix with Liquid Time the program waits after each aspirate and dispense before moving out of 0 min Equilibration the well Time Mix with Liquid Select Touch Off to activate the Side or Surface Touch Off within the Tips tab Touch Off Mix with Air The quantity of air used as part of a mixing process OuL Mix Volume Mix with Air The quantity of an air gap 20 uL Air Gap Mix with Air The speed at which the Mix Volume moves into and out of the probe For a range 5 mL min Flow Rate of suggested flow rate values see Flow Rates on page 4 93 Mix with Air Time the program waits after each aspirate and dispense before moving out of 0 min Equilibration the well Time Mix with Air Select Touch Off to activate the Side or Surface Touch Off within the Tips tab Touch Off Mix with Tips Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide C 51 sdi L yum XIW Mix with Tips Tasks Disposable Tips Mix with Tips Tips Property Name Brief Description Default
222. age 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into and out of the 3 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Fractionate Properties Table Page 1 of 5 TRILUTION LH Software Version 2 0 User s Guide D 53 9 euU01 Ie14 Fractionate Tasks SPE D Fractionate Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Air Gap moves into and out of the probe For a 1 mL min Air Gap Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select the Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample
223. age Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Oo ON WD MN B amp B Syringe Move probe to DEC Well in DEC Zone Move Z to top Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top N OW A U N gt Dispense Air Push Volume at Dispense Flow Rate D 40 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 8 Wait Equilibration Time 9 Move Zto top performing shake off SPE 215 10 Repeat steps 4 9 until Air Push Volume is dispensed 11 Move probe to Inside Rinse Wells at Inside Rinse Zone 12 Dispense Air Gap at Dispense Flow Rate Valve Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top CON AO WM BP
224. age for that instrument TRILUTION LH Software Version 2 0 User s Guide 4 3 MOPUIM qe UO eANHIyUuO gt D Configuration Tab Window Method Builder Configuration 4 4 4 Communication Used only for navigation Click on the instrument name to view the property page for that instrument Injector Used only for navigation Click on the instrument name to view the property page for that instrument Instrument Types Lists the available instrument groups Available Instruments Lists the instruments of the selected instrument group or those identified during the scan Workspace Area used to create or view a Configuration Properties Area used to view and or edit instrument properties TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 4 Creating a Configuration To create a Configuration 1 Doone of the following e From the Instrument Types select an instrument group Liquid Handlers Pumps Accessories Communication Injector or All Instruments Available instruments for the selected Instrument Type are listed in the Available Instruments window e Click SCAN TRILUTION LH searches for Gilson instruments that are powered ON and are connected to the Gilson Serial Input Output Channel GSIOC Results of the scan and any Ethernet instruments are displayed in the Available Instruments window e Click BROWSE Select an existing Configuration and click OK 2 From the
225. aining Training courses are offered regularly at Gilson or a customized training course can be brought to your facility In addition complimentary training through use of Gilson s Quick Start Tutorial provides you with immediate access to how to movies The following TRILUTION LH training has been developed to specifically meet different experience levels Quick Start Tutorial Let Gilson s Quick Start Tutorial assist you with learning and using TRILUTION LH Software Your purchase of a TRILUTION LH license includes the Quick Start Tutorial on CD ROM which automatically loads the tutorial s main menu Six TRILUTION LH Software tutorials and an introductory tutorial are available for you to learn how to program TRILUTION LH Software for a Gilson liquid handling application The tutorials can be launched in any order by clicking on each selection from the main menu Introductory TRILUTION LH Training TRTRILUTION1 2 days Two days of classroom training with TRILUTION LH provides participants with the basic tools needed to operate and understand their Gilson liquid handling system The majority of training time is spent performing applications using the supplied tasks within the TRILUTION database This training course is a complete and necessary introduction for the novice system and software user TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview 1 Course Objectives After attending
226. al Control Continued Property Name Brief Description Default Value Use X Enter anumber to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm l Click MOVE Z value Output Contacts 24V A filled box indicates that the circuit is Toggle the contacts open closed off 1 closed on An empty box indicates that on by selecting and clearing the 2 the circuit is open off boxes 3 4 Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the C circuit is open D Pump Prime Flow Rate The speed at which the reservoir fluid 10 mL min CiiciacniMEResiareOreror rined moves into and out of the syringe SPE 215 Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 71 MOpUIM qe J01 Uu0 enuey Manual Control Tab Window Application Manual Control SPE 215 Manual Control Continued Property Name Brief Description Default Value Use Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position HOME SYRINGE Homes the d
227. allows the software to communicate to the instrument 506C System Interface Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 35 uoljeinbyuo e Hurzeadly Creating a Configuration Method Builder Configuration 606 GSIOC to RS 232 Converter Hardware C Software C Both None The 606 GSIOC to RS 232 Converter Properties Window Properties 606 GSIOC to RS 232 Converter Properties Property Name Brief Description Default Value Range Setup Channel A Baud Rate The speed at which information is sent 19200 75 110 150 300 600 1200 1800 2000 2400 4800 9600 19200 38400 Parity The number of 1s in the group of bits Even Even Odd None M 1 S 0 606 GSIOC to RS 232 Converter Properties Table Page 1 of 3 4 36 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 606 GSIOC to RS 232 Converter Properties Continued Property Name Brief Description Default Value Range Bits per Character The number of binary digits that constitutes a 7 5 6 7 8 character Stop Bits The number of bits to be transmitted after each 1 1 2 character Handshake The device with which information is being None Hardware Software Both transmitted or exchanged None Setup Channel B Baud Rate The speed at which information is sent 19200 75 110 150 300 600 1200 1800 2000 2400 4800 9600 19200 38400 Parity Th
228. alues see Flow Rates on page 4 93 Elute Properties Table Page 2 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Elute Properties Continued Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate DEC Zone The zone to which the Reservoir Volume is delivered DEC Well The well in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 3 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 1 min Time SPE 215 Shake The SPE 215 Shake Off unseals the seal foot from the mobile rack Off Reset Mobile The reset mobile rack moves the mobile rack over the drain Rack Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Elute Properties Table Page 3 of 5 TRILUTION LH Software V
229. anual Control Continued Property Name Brief Description Default Value Use X Enter a number to move to a position to 0 0 mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm Click MOVE Z value Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the 3 the circuit is open off boxes 4 24V Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the 3 the circuit is open off boxes 4 GX 281 Liquid Handler Manual Control Table Page 2 of 4 9 60 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control GX 281 Liquid Handler Manual Control Continued Property Name Brief Description Default Value Use Input Contacts A A filled box indicates that the circuit is B closed An empty box indicates that the C circuit is open D Pump Monitor status Valve Position Rotates the solvent selection valve to the selected position Prime Flow Rate The speed at which the solvent moves out of the probe Injection Valve Indicate which GX Direct Inject Valve is installed if any Val
230. are that allows the software to communicate to the instrument ASPEC XL Properties Table Page 2 of 2 10 0 31 TRILUTION LH Software Version 2 0 User s Guide 4 41 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration 4 ASPEC XL4 Syringe Name Size pL Reservoir Name A 10000 Reservoir B 10000 Reservoir c 10000 Reservoir D 10000 Reservoir The ASPEC XL4 Properties Window Properties ASPEC XL4 Properties Property Name Brief Description Default Value Range Setup Z Safe Height mm The Z height at which the instrument s 0 183 to O probe moves to before moving in the X or Y direction Probes to Use The probes and syringes that will be A B C D A B C D used when this instrument is selected as the Liquid Handler and Pumpin a Task ASPEC XL4 Properties Table Page 1 of 3 4 42 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration ASPEC XL4 Properties Continued Property Name Brief Description Default Value Range ID A unique identifier to address syringes A B C D A B C D on the instrument Syringe Name A unique name to address syringes on A B C D N A the instrument Size uL The capacity of each installed syringe 10000 500 1000 5000 10000 25000 For a range of suggested flow rates for this syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the Res
231. arning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons Quad Z 215 Manual Control Ais n Brief Description Default Value Use Liquid Handler Active Probes The probes that will be used A B C D Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number 2 mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom Quad Z 215 Manual Control Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 67 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control Quad Z 215 Manual Control Continued ore Brief Description Default Value Use Name X Enter a number to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm l Click MOVE Z value Output Contacts 24V A filled box indicates that the circuit is Toggle the contacts open closed off 1 closed on An empty box indicates that on by selecting and clearing the boxes 2 the circuit is open off 3 4 Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the C c
232. ask aspirates the specified volume of liquid source from the reservoir tray or transfer ports and another liquid solution from the tray and then dispenses the combined volume to the wells of the result zone Aliquot This task aspirates the liquid source from the reservoir tray or transfer ports and then dispenses that liquid equally to each of the wells of the result zone This task should be used in Batch mode only Cherry Pick This task transfers liquid source from wells in the source zone to a sequence of wells beginning with the starting well in the result zone Derivatize This task performs an add aspirates the specified volume of liquid source from the reservoir tray or transfer ports and another liquid solution from the tray and then dispenses the combined volume to the wells of the result zone and then mixes with liquid or air Liquid Handling Tasks Table Page 1 of 3 A 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Liquid Handling Tasks Continued Dilute This task aspirates the specified volume of liquid source from the tray and another liquid diluent from the reservoir tray or transfer ports and then dispenses the combined volume to the wells of the result zone Dispense This task aspirates the specified volume of liquid source from the reservoir tray or transfer ports and then dispenses the volume to the wells of the
233. at Dispense Flow Rate Valve CON AO MN BP WN 9 Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe TRILUTION LH Software Version 2 0 User s Guide Aig Dry Tasks SPE 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min INSIDE RINSE Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate mM A WN gt Move Z to top OUTSIDE RINSE Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Height from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate mM A U N Move Z top GAS AIR PURGE If SPE 215 TRUE 1 Move Z to top 2 Set Voltage On and Output Contact 1 to Close 3 Wait 0 02 min D 26 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 4 Set Voltage Off and Output Contact 1 to Open 5 Move Z to top TRILUTION LH Software Version 2
234. at which the Source Volume and Extra Volume move into and out of the 5 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into and out of the probe For a 0 3 mL min Air Gap Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select the Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 25 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into and out of the 5 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Load and Collect Properties Table Page 2 of 5 TRILUTION LH Software Version 2 0 User s Guide D 85 33 0 gt pue peo Load and Collect Tasks SPE D Load and Collect Properties Continued Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into and out of
235. ate to the instrument VALVEMATE Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 85 uoleinbyuo e Nurze Creating a Configuration Method Builder Configuration 4 VALVEMATE II The VALVEMATE II Properties Window Properties VALVEMATE II Properties Property Name Brief Description Default Value Range Setup Valve Positions The number of ports in the VALVEMATE valve 2 2 4 6 8 10 Initial Valve Position The valve position when homed 1 1 2 VALVEMATE II Properties Table Page 1 of 2 4 86 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration VALVEMATE II Properties Continued General Instrument Name The name that will be used to identify this Valve N A instrument in a Task GSIOC ID A unique numerical address that must be known 35 30 39 to the instrument and to the software that allows the software to communicate to the instrument VALVEMATE II Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 87 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration Virtual Pumping System The Virtual Pumping System VPS is a group of instruments that are combined to perform as a pumping system A VPS consists of probes from a liquid handler and syringes Optionally a VALVEMATE may be included to provide additional fluid paths The Con
236. ated Maximum Flow Rates Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Tubing Options and Associated Maximum Flow Rates Tubing Maximum Flow Rate mL min Fluoroelastomer 4 mm 33 92 Silicone 0 6 mm 1 58 Silicone 1 mm 4 13 Silicone 2 mm 14 94 Silicone 2 8 mm 21 73 Polypropylene 0 5 mm 1 48 Polypropylene 1 mm 4 74 Polypropylene 2 mm 15 52 High Flow PVC 2 06 mm 24 7 PVC 2 29 mm 30 08 PVC 2 79 mm 42 55 PVC 3 16 mm 49 59 PVC 4 0 mm 80 94 PVC 5 0 mm 96 PVC 6 0 mm 157 89 PVC 8 0 mm 209 61 Tubing Options and Associated Maximum Flow Rates Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 73 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration Tubing Options and Associated Maximum Flow Rates Tubing Maximum Flow Rate mL min Fluoroelastomer 2 0 mm 23 9 Fluoroelastomer 3 0 mm 48 34 Fluoroelastomer 4mm 74 19 Fluoroelastomer 6 mm 153 85 Silicone 2 mm 25 72 Silicone 3 mm 45 45 Silicone 4 mm 68 77 Silicone 5 mm 106 19 Silicone 6 0 mm 150 94 Silicone 7 0 211 45 Polypropylene 2 mm 27 01 Polypropylene 3 2 mm 55 05 Polypropylene 6 4 169 61 Tubing Options and Associated Maximum Flow Rates Table Page 3 of 3 4 74 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Multiple Probe 215
237. ated date and created by configuration information bed layout information racks used variables steps and error handling Application This report comprises two parts a brief summation of the application information application name and version created by created date and description and sample list details including values for variables This report comprises three parts a brief summation of the application run information application name application version run name run date run by and run mode sample list details including values for variables captured at the end of the run and log information Sample Tracking This report comprises a brief summation of the application run information application name and version run name run date run by and run mode and the aspirate and dispense actions for each sample TRILUTION LH Software Version 2 0 User s Guide 11 3 MOPpUIM syod y yL The Reports Window Generating Reports 11 4 11 Task Report This report comprises three parts a brief summation of the task information name version description modified date and created date variables defined for the task Variables and the un timed sequential steps Steps Refer to the example on the next page TRILUTION LH Software Version 2 0 User s Guide Generating Reports Task Report Task Name Asprate Version i Description Created Dane TI GO VORIS AM
238. ation Time oO Ww Perform a Touch Off X direction if selected and move Z to Z Safe Height TRILUTION LH Software Version 2 0 User s Guide A 63 su ds q Dispense Tasks Liquid Handling Tray or Transfer Port Move probe to Result Well in Result Zone Lower probe into well to Result Height Wait Equilibration Time 1 2 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected 4 5 Perform a Touch Off X direction if selected and move Z to Z Safe Height dispense until Source Volume is delivered OV Move probe to well in Inside Rinse Zone N Lower probe into well to Inside Rinse Height oe Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate 5 Dispense Inside Volume at Inside Rinse Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe into well in Outside Rinse Zone A 64 If Source Volume is greater than syringe capacity repeat aspirate Air Gap and Extra Volume are not aspirated and TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Fl
239. be will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low 1 setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Cherry Pick Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide A 27 Id Kay Cherry Pick Tasks Liquid Handling Cherry Pick Advanced Rinsing Continued A Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at
240. ber is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Cherry Pick with Tips Tips Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Cherry Pick with Tips Tips Continued Property Name Brief Description Default Value Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Cherry Pick with Tips Tips Table Page 3 of 3 Cherry Pick with Tips Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Active Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List wh
241. ber is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Dispense Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 81 su ds d SS SS Dispense Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Dispense Advanced Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Dispense Advanced Outside Rinse Table Page 1 of 4 B 82 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Dispense Advanced Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid leve
242. between the probe and the valve Volume of an airgap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 01 min Equilibration Time Dry Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide D 21 Aig gt e O Tasks SPE D Dry Advanced Rinsing Property Name Brief Description Default Value Air Push Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the prob
243. can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Solution Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Solution Volume Derivatize Advanced Rinsing Table Page 1 of 3 2mm TRILUTION LH Software Version 2 0 User s Guide aZNeALIAG Derivatize Tasks Liquid Handling Derivatize Advanced Rinsing Continued A Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone and mixing Inside Rinse The zone to which the Inside Volume is delivered In
244. cation displays with errors see log for details TRILUTION LH Software Version 2 0 User s Guide 7 7 Buljpuepy 10113 JUaWINAYSU dN Bus Modifying Instrument Error Handling 7 8 Method Builder Error Handling 7 Modifying Instrument Error Handling You can modify instrument error handling by doing the following Setting up error handling for additional instruments in the Configuration e Setting up error handling with additional criteria for the same instrument e Deleting instruments and or parameters from the monitored list of criteria e Modifying the instruments and or parameters in the monitored list of criteria When you right click on a row in the table of monitored criteria a menu is displayed with the option to delete the criteria To make a modification select the row make the desired changes and then click MODIFY Clear Default Error Name When a Method is saved the Error Handler is named An option is provided to set that Error Handler as a default for future new Methods Click CLEAR DEFAULT to remove an Error Handler as default TRILUTION LH Software Version 2 0 User s Guide Application 8 In the Application window you specify the list of steps called a Sample List to execute during a run A step in the Sample List identifies a sample to be processed and the Method that includes the steps to process that sample This section describes the components of the Application window
245. ce Well to Source Height 4 5 Move Z to top Transfer Port 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well in Source Zone 3 Lower probe to Source Height from Tube Bottom 4 Set Transfer Port to On 5 Aspirate Source Volume Extra Volume at Source Flow Rate 6 Set Transfer Port to Off 7 Move Z to top D 110 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE DISPENSE Tray Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time mM A WN gt Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume delivered 6 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top 7 Move probe to well in Inside Rinse Zone 8 Lower probe to Inside Rinse Height from Tube Bottom 9 Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top TRILUTION LH Software Version 2 0 User s Guide D 111 ysem Wash Tasks SPE Transfer Port Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time mM A U N gt Move Z to top Dispense until Source Volume delivered 6 IfSPE215 TRUE perform Shake Off a
246. ce point Tube Bottom when delivering to the Injection Zone Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Inject Advanced Inside Rinse Table Page 2 of 3 B 104 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Inject Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to t
247. ce up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Mix Advanced Rinsing Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Mix Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Mix Instruments Mix Sequence of Steps The following is an overview for the sequence of steps A U N gt MIX WITH LIQUID N OAU A WU N gt Mix with Liquid on page A 85 Mix with Air on page A 86 Inside Rinse on page A 87 Outside Rinse on page A 87 Move Z to Z Safe Height Aspirate Air Gap at Air Gap Flow Rate Move probe to Mix Well in Mix Zone Lower probe into well to Source Height Aspirate Mix Volume at Flow Rate using Liquid Level Following if selected Lower probe into well to Result Height Dispense Mix Volume at Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User
248. ce up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Dilute Advanced Rinsing Table Page 3 of 3 Dilute Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Dilute Instruments TRILUTION LH Software Version 2 0 User s Guide A 51 anid Dilute Tasks Liquid Handling A Dilute Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 52 2 Dispense on page A 54 3 Inside Rinse on page A 55 4 Outside Rinse on page A 55 ASPIRATE Reservoir 1 Move Z to Z Safe Height 2 Aspirate Diluent Volume from Reservoir at Diluent Flow Rate 3 Move probe to Source Well in Source Zone 4 Aspirate Source Air Gap at Air Gap Flow Rate 5 Lower probe into well to Source Height 6 Aspirate Source Volume at Source Flow Rate using Liquid Level Following if selected 7 Move Z to Z Safe Height Tray 1 Move Z to Z Safe Height 2 Move probe to Source Well in Source Zone 3 Aspirate Source Air Gap at Air Gap Flow Rate 4 Lower probe into well to Source Height A 52 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Aspirate Source Volume Extra
249. chapters are sequenced based on the TRILUTION LH functionality The following table lists the material covered in each chapter Chapter Name TRILUTION LH Software Overview Provides a TRILUTION LH overview Task Builder Provides information on how to build a Task Method Builder Provides information about the Method Builder Method Builder Configuration Provides information on how to select instruments and set their properties to build a Configuration as part of a Method Method Builder Bed Layout Provides information on how to design a Bed Layout as part of a Method Method Builder Method Provides information about how to build a Method Method Builder Error Handling Provides information about how to set up error handling as part of a Method Application Provides information on how to build an Application Application Manual Control Provides information about how to send manual commands to an instrument in a Configuration Working with Variables Provides information on how to use Variables Operators and build Expressions Generating Reports Provides information about viewing and exporting reports Chapter Name Tasks Liquid Handling Provides a list and description of the Liquid Handling Tasks Tasks SS Liquid Handling Provides a list and description of the SS Liquid Handling Tasks Tasks Disposable Tips Provides a list and description o
250. ck and the wells or elements to be used on the rack Either create custom wells or elements or use the pre defined wells and elements provided by Gilson Wells and elements can be added individually or as an array Information stored concerning the rack includes its physical dimensions the type of wells that it holds and their location and the templates on which the rack can be placed Keywords e Element A rack element is a footprint or a well e Footprint Area that accepts racks e Wells Wells specifically custom wells can only go in racks e Rack A rack requires a footprint before it can be added to a template Examples of racks are Racks Rinse Stations Injection Port Bars Tip Boxes and Microplates e Template The template is the bottom a required layer of a Bed Layout Only footprints can be added to templates e Handle A position designated in a rack which makes the rack mobile Zones cannot be assigned to a handle e Target A position defined within a well Zones can be assigned to a target TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Creating a Rack 1 Click NEW RACK button the New Rack dialog appears BJ New Rack 2 Inthe New Rack window set the rack shape Rectangle or Circle TRILUTION LH Software Version 2 0 User s Guide s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities H 3 Enter the dimensions a For a rectangular
251. close the Task Builder click E on the upper right corner of the Task Builder 2 20 TRILUTION LH Software Version 2 0 User s Guide Method Builder To begin creating a Method select METHOD BUILDER from the Liquid Handling menu TRILUTION LH Software Version 2 0 User s Guide 3 1 Method Builder Method Builder 3 2 Method Builder Using the options in the Method Builder you specify the Configuration a Bed Layout and method conditions for controlling instruments Action Buttons The following action buttons are located in the lower left corner of the Method Builder Action Button Description NEW Create a new Method OPEN Open an existing Method SAVE Save an existing Method to the same name Optionally modify the Configuration Bed Layout Name or Error Name and specify the Configuration or Error Name as default SAVE AS Save a new Method or save an existing Method to a new name Optionally type a description or modify the Configuration Bed Layout Name or Error Name and specify the Configuration or Error Name as default MODIFY Modify the description for the Method TRILUTION LH Software Version 2 0 User s Guide Method Builder Action Button Description DELETE Deletes the open Method or all versions of the Method EXPORT Exports the Method to a specified location as a LHME file IMPORT REFRESH Imports a LHME file and lists the Method in the Methods palett
252. collect zone Load If analytes of interest bind to the column use this task to aspirate a specified volume of liquid source from the reservoir tray or transfer ports and then dispense the volume to the wells of the DEC zone with the mobile rack positioned over the drain Load and Collect If the contaminants bind to the column and the analytes of interest pass through the column into the collect zone use this task to aspirate the specified volume of liquid source from the reservoir tray or transfer ports and then dispense the volume to the wells of the DEC zone with the mobile rack over the collect position Move Mobile Rack This task initializes the mobile rack or moves over the drain or the collect Wash This task washes the DEC by aspirating a specified volume of liquid source from the reservoir tray or transfer ports and then dispensing the volume to the wells of the DEC zone with the mobile rack positioned over the drain SPE Tasks Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide D 3 Condition Tasks SPE D Condition This task prepares the DEC to accept a prepared sample by aspirating then dispensing liquid source from the reservoir tray or transfer ports with the mobile rack positioned over the drain Properties Advanced ttinsing fimstruments l FOr more information about the properties on each tab see Condition ool Condition Properties on page
253. contents in a column click in the first cell and drag down through the cells to increment The cursor displays as a plus sign briefly when the value can be incremented To copy the contents of one cell to others in the same column click in the cell with contents to be copied and then hold CTRL and drag down through the cells to which the contents should be copied The cursor displays as a plus sign briefly when the value can be copied To copy the contents of one row to another select the row to be copied and then when the cursor changes drag and drop the row at the destination 8 14 TRILUTION LH Software Version 2 0 User s Guide Application Not all columns and rows allow copying and incrementing Refer to the following table Increment Column COPY Contents Eee Copy Contents of One Cell of Row Column Method NO NO YES Name Mode YES COPIES YES Sample YES COPIES YES Description TRILUTION LH Software Version 2 0 User s Guide 8 15 SI ajdwes e dn bulyjas Setting Up a Sample List Application 8 16 Default Columns When anew Sample List is created columns for Method Name Mode and Sample Description appear by default Columns can be shown or hidden Right click on a column name and then select the column name from the menu Doing so toggles the column to hide Repeat to toggle the column to show Columns can be re sized by dragging the column border in the column hea
254. ct Valve None is installed Valve Position Click SWITCH Rotates the injection valve to the selected position Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors GX 271 Liquid Handler Manual Control Table Page 3 of 4 9 48 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 GX 271 Liquid Handler Manual Control Continued Default Value Use Brief Description Property Name LOCK ARM Powers all motors and moves the probe to the home position HOME PUMP Homes the solvent system GX 271 Liquid Handler Manual Control Table Page 4 of 4 TRILUTION LH Software Version 2 0 User s Guide 9 49 MOPpUIM qe JO4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 9 GX 271 Liquid Handler without Pump The GX 271 Liquid Handler without Pump Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons GX 271 Liquid Handler without Pump Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number 2mm Click MOVE TO WELL
255. cted Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Solution Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Solution Volume SS Derivatize Advanced Inside Rinse Table Page 1 of 3 2mm TRILUTION LH Software Version 2 0 User s Guide B 49 9ZILALIAG SS SS Derivatize Tasks SS Liquid Handling SS Derivatize Advanced Inside Rinse Continued Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result
256. cted when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No rinse is performed 0 uL when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe No rinse is performed 0 uL when the Outside Volume is 0 Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Dispense Random to Random with Tips Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide C 23 sdij YIM WopueY 0 wopuey asuadsig Dispense Random to Random with Tips Tasks Disposable Tips Dispense Random to Random with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for ri
257. ction if selected and move Z to Z Safe Height Tray 1 2 3 4 Move probe to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Source Volume Diluent Air Gap Diluent Volume at Result Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User s Guide B 73 23Nn I0 SS SS Dilute Tasks SS Liquid Handling B Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height 5 6 7 Move probe to Inside Rinse Well in Inside Rinse Zone 8 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 9 Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to Inside Rinse Well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 4 Switch solvent valve to Inside Rinse Solvent Valve Position 5 Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Positio
258. d Max Flow Rate mL min channel Associated Maximum Flow Rates on page 4 72 Reservoir Name A unique name used to identify the Reservoir N A liquid that is flowing through the Minipuls It is important for sample tracking Instrument Name The name that will be used to identify Minipuls 3 N A this instrument in a Task Reservoir Name The name that will be used to identify Reservoir N A the liquid that is flowing through the associated syringe It is important for sample tracking General Pump Name The name that will be used to identify Pump this pump in a Task GSIOC ID A unique numerical address that must 30 0 63 be known to the instrument and to the software that allows the software to communicate to the instrument Minipuls 3 Properties Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 71 uoleinbyuo e Gbuljeal gt Creating a Configuration Method Builder Configuration 4 72 4 Tubing Options and Associated Maximum Flow Rates Tubing Maximum Flow Rate mL min Standard PVC 0 25 mm 0 41 PVC 0 38 mm 0 69 PVC 0 51 mm 1 42 PVC 0 76 mm 2 15 PVC 1 02 mm 4 06 PVC 1 3 mm 6 14 PVC 1 52 mm 9 09 PVC 1 65 mm 10 46 PVC 2 06 mm 15 1 PVC 2 29 mm 18 79 PVC 2 79 mm 22 17 PVC 3 16 mm 27 52 Fluoroelastomer 0 5 mm 1 77 Fluoroelastomer 1 mm 3 87 Fluoroelastomer 2 mm 12 54 Fluoroelastomer 3 mm 28 25 Tubing Options and Associ
259. d surface This selection changes the reference point for the Source Height Diluent Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Diluent Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Diluent Volume Dilute Advanced Rinsing Table Page 1 of 3 2mm TRILUTION LH Software Version 2 0 User s Guide s3niid 7 E om Tasks Liquid Handling Dilute Advanced Rinsing Continued A Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm nu
260. d Builder Property Name Z Safe Height mm Configuration SPE 215 Properties Continued Brief Description The Z height at which the instrument s probe moves to before moving in the X or Y direction Default Value 178 Range Calculated based on Z Clamp Height and Z Arm Type Range provided on screen Configuration The number of probes and syringes that will be used when selected as the Liquid Handler in a Task 8 Probe Configuration 4 Probe Configuration 8 Probe Configuration Setup Syringe Pump ID A unique identifier to address syringes on the instrument A B C D E F G H A B C D E F G H Syringe Name A unique name to address syringes on the instrument A B C D E F G H A B C D E F G H Size uL The capacity of each installed syringe Fora 1000 500 1000 2500 5000 range of suggested flow rates for this syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid Reservoir N A that is flowing through the associated syringe It is important for sample tracking SPE 215 Properties Table Page 2 of 3 4 82 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration SPE 215 Properties Continued Property Name Brief Description Default Value Range uoleinbyuo e Nurze Contacts 24V Output Optionally type a description for the contact N A N
261. d Handling Derivatize Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample O uL Source Volume Reservoir The speed at which the Source Volume moves into the probe For a range of 10 mL min Source Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where xis a value defined in the Sample List Tray The quantity of a sample O uL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Derivatize Properties Table Page 1 of 4 A 33 TRILUTION LH Software Version 2 0 User s Guide aZNeALAG Derivatize Tasks Liquid Handling Derivatize Properties Continued A Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page
262. d Handling B INSIDE RINSE mM A WN gt 6 Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Switch solvent valve to Inside Rinse Solvent Valve Position Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume Move Z to Z Safe Height OUTSIDE RINSE mM A WN gt B 32 Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off Move Z to Z Safe Height Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B SS Cherry Pick This task transfers liquid source from wells in the source zone to a sequence of wells beginning with the starting well in the result zone For more information about the properties on each tab
263. d Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated This Following selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tubes This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Load Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load Advanced Rinsing Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 18 mm number is en
264. d moves the probe to the home position 215 SW Liquid Handler Manual Control Table Page 2 of 2 9 10 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 221 XL Liquid Handler The 221 XL Liquid Handler Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 221 XL Liquid Handler Manual Control peer Brief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Click MOVE TO WELL Z Offset The distance up when a positive number is entered ordown 2mm when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right of the home 0 0 mm position Click MOVE X Y Y Enter a number to move to a position forward of the home 0 0 mm position Z Enter a number to move to an absolute Z value 0 0 mm Click MOVE Z 221 XL Liquid Handler Manual Control Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 11 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 9 221 XL Liquid Handler Manual Control Continued Name Relay Outputs 1 A filled box indicates that the circuit is closed on An empty Toggle the contacts 2 box indicates that the circuit is open off op
265. d to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Mix Advanced Inside Rinse Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide B 117 XIW SS SS Mix Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Mix Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value D
266. d volume of liquid source from the reservoir tray or transfer ports and then dispenses the volume to the wells of the result zone Liquid Handling Tasks Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Add This task aspirates the specified volume of liquid source from the reservoir tray or transfer ports and another liquid solution from the tray and then dispenses the combined volume to the wells of the result zone Properties PAdwanend Rinsing Pinsin S For more information about the properties on each tab see o Add Properties on page A 6 Add Advanced Rinsing on page A 9 Add Instruments on page A 11 Add Sequence of Steps on page A 12 TRILUTION LH Software Version 2 0 User s Guide A 5 PPV Add Tasks Liquid Handling Add Properties A Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into the probe For a range of 10 mL min Source Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity o
267. de Tasks SS Liquid Handling SS Transfer Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Transfer Outside Rinse Table Page 3 of 4 B 153 TRILUTION LH Software Version 2 0 User s Guide JBJSUCAL SS SS Transfer Tasks SS Liquid Handling SS Transfer Outside Rinse Continued Property Name Brief Desc
268. de of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Prime Transfer Ports Rinsing Table Page 2 of 2 Prime Transfer Ports Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Prime Transfer Ports Instruments TRILUTION LH Software Version 2 0 User s Guide A 101 S 10d JajSueAL BW Prime Transfer Ports Tasks Liquid Handling Prime Transfer Ports Sequence of Steps The following is an overview for the sequence of steps 1 2 3 Aspirate Dispense on page A 102 Inside Rinse on page A 103 Outside Rinse on page A 103 ASPIRATE DISPENSE CON AO WM BP WN 9 Move Z to Z Safe Height As
269. defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Aliquot Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 25 yonbijy SS SS Aliquot Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Aliquot Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Aliquot Outside Rinse Table Page 1 of 4 B 26 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside R
270. der Shown columns and selected widths are saved when the Application is saved Descriptions of all columns follow Method Name This column is required Select a Method from the drop down list of Methods that use the selected Bed Layout This is the Method that will be used when the selected sample s are run TRILUTION LH Software Version 2 0 User s Guide Application Mode This column is required Right click the cell to choose the mode There are two choices Batch and Sequential that are described below The default is Sequential Batch In Batch B mode all samples are processed in order using the first Task in the Method Then all samples are processed in order using the second Task in the Method and so on Sequential In Sequential S mode each sample is fully processed using all Tasks in the Method before continuing to the next sample Sample Description This column is optional Click in the cell and type a name that describes the Sample default is Sample Description Additional Columns Other columns appear depending on the variables set in your Method TRILUTION LH Software Version 2 0 User s Guide 8 17 SI ajdwes e dn bulyjas Exporting a Sample List Application Exporting a Sample List 1 On the Application window click the Sample List Export IF button 2 Select a folder and then click SAVE The Sample List is saved in the specified folder with a TSL extension TRI
271. dilutor select the liquid handler Cherry Pick Instruments TRILUTION LH Software Version 2 0 User s Guide A 29 Id Kay Cherry Pick Tasks Liquid Handling A Cherry Pick Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 30 2 Dispense on page A 30 3 Inside Rinse on page A 31 4 Outside Rinse on page A 31 ASPIRATE 1 Move Z to Z Safe Height 2 Aspirate Source Air Gap at Air Gap Flow Rate 3 Move probe to Source Well in Source Zone 4 Lower probe into well to Source Height 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 6 Move Z to Z Safe Height DISPENSE 1 Move probe to Starting Well in Result Zone Note Well is automatically incremented for subsequent Result Wells when a variable is used for Result Well Lower probe into well to Result Height Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height ma A U N A 30 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A 6 If Source Volume is greater than syringe capacity repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume is delivered 7 Move probe to well in Inside Rinse Zone 8 Lower probe into well to Inside Rinse Height 9 Dispense Extra Volume Source Air Gap at Result
272. direction forward Move probe to Side Rinse Y Offset Move probe to the center of the well Move Z to top TRILUTION LH Software Version 2 0 User s Guide C 59 sdi L yum XIW Mix with Tips Ta sks Disposable Tips Surface 1 2 3 4 Move Z to top Move probe to well in Outside Rinse Zone Move probe to Surface Rinse Z Offset Move Z to top None 1 C 60 Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Prime Pump with Tips This task primes the pump in the specified zone It aspirates the specified volume or the syringe capacity from the reservoir and then dispenses to the wells in the result zone Properties Advanced Rtiming festruments For more information about the properties on each tab see Prime Pump with Tips eo Prime Pump with Tips Properties on page C 62 Prime Pump with Tips Advanced Rinsing on page C 62 Prime Pump with Tips Instruments on page C 64 Prime Pump with Tips Sequence of Steps on page C 65 TRILUTION LH Software Version 2 0 User s Guide C 61 sdi yyim dwing awd Prime Pump with Tips Tasks Disposable Tips Prime Pump with Tips Properties Property Name Brief Description Default Value Source Source for the Prime Pump is reservoir Reservoir Prime Volume The quantity of a sample OuL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 10
273. dler See page 4 51 GX 271 Liquid Handler without Pump See page 4 55 GX 274 ASPEC without Pump See page 4 59 GX 274 Liquid Handler without Pump See page 4 62 GX 281 Liquid Handler See page 4 65 Minipuls 3 See page 4 69 Multiple Probe 215 See page 4 75 Quad Z 215 See page 4 78 SPE 215 See page 4 81 VALVEMATE See page 4 84 VALVEMATE II See page 4 86 Virtual Pumping System See page 4 88 TRILUTION LH Software Version 2 0 User s Guide 4 7 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration 4 215 Liquid Handler 77th ID Syringe Name Size uL Reservoir Name gt A A 1000 Reservoir The 215 Liquid Handler Properties Window Properties 215 Liquid Handler Properties Property Name Brief Description Default Value Range Setup 215 Liquid Handler Z Arm Type mm This is the height of the Z arm installed 125 mm Z Arm 125 mm Z Arm 175 mm Z Arm on the liquid handler Z Clamp Height mm This number corresponds to where the 125 0 175 mounting clamp is on the vertical ruler on the installed Z arm 215 Liquid Handler Properties Table Page 1 of 3 Range provided on screen 4 8 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 215 Liquid Handler Properties Continued Property Name Brief Description Default Value Range Z Safe Heig
274. dler without Pump Manual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 9 7 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control 9 215 Liquid Handler without Pump Manual Control Continued oe Brief Description Default Value Use Name Output Contacts 24V A filled box indicates that the circuit is closed on An empty Toggle the contacts 1 box indicates that the circuit is open off open closed off 2 on by selecting 3 and clearing the 4 boxes Input Contacts A A filled box indicates that the circuit is closed An empty box Monitor status B indicates that the circuit is open C D Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position 215 Liquid Handler without Pump Manual Control Table Page 2 of 2 9 8 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 215 SW Liquid Handler rests eon eOvoeoen Th e 215 SW Liquid Handler Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 215 SW Liquid Handler Manual Control Property B
275. dler without Pump Properties Continued Property Name Brief Description Default Value Range uoleinbyuo e Gules Contacts Output 1 Optionally type a description forthe N A N A Output 2 contact This description will be used 24V Output 1 to identify the contact in a Task a Additionally indicate whether the contact should open close or remain in the same state on error The default is No Change for Outputs 1 and 2 and Open for 24V Outputs 1 and 2 Input A Optionally type a description forthe N A N A Input B contact This description will be used to identify the contact in a Task Solenoid 1 Optionally type a description forthe N A N A Solenoid 2 solenoid This description will be used to identify the solenoid ina Task General Instrument Name The name that will be used to Liquid Handler N A identify this instrument in a Task GX 271 Liquid Handler without Pump Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Creating a Configuration Method Builder Configuration GX 271 Liquid Handler without Pump Properties Continued Property Name Brief Description Default Value Range Instrument Serial Number The serial number of the instrument N A N A Injection Module Serial Number The serial number of the injection N A N A module GX 271 Liquid Handler without Pump Properties Table Page 3 of 3 4 58 TRILUTION LH Software Version
276. dling SS Aliquot Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Result Volume is delivered Result Well The well in the Result Zone to which the Result Volume is delivered 1 Result Volume The quantity of liquid delivered to the Result Well in the Result Zone O uL Result Flow Rate The speed at which the Result Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction SS Aliquot Properties Table Page 2 of 2 B 22 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Liquid Level Following SS Aliquot Advanced Inside Rinse Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Default Value Liquid Level Detection When selected
277. dling A 4 5 6 Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Transfer Port CON OWN RA UW N Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to well in Source Zone Lower probe into well to Source Height Set Transfer Port to On Aspirate Source Volume Extra Volume at Source Flow Rate Set Transfer Port to Off Move Z to Z Safe Height DISPENSE Reservoir 1 2 3 4 oO U Move probe to Result Well in Result Zone Lower probe into well to Result Height Aspirate Source Volume from Reservoir at Source Flow Rate Dispense Source Volume at Result Flow Rate using Liquid Level Following is selected If Source Volume is greater than syringe capacity repeat steps 3 4 until Source Volume is delivered Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height A 114 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Tray or Transfer Port Move probe to Result Well in Result Zone Lower probe into well to Result Height 1 2 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following is selected 4 Wait Equilibration Time 5 Perform a Touch Off X direction if selected and move Z to Z Safe Height If Source Volume is greater than syringe capacity repeat aspirate and dispense Air Gap Extra V
278. e 1000 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Dry Advanced Rinsing TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Property Name Liquid Handler Dry Instruments Brief Description The liquid handler that the Task will affect D Default Value Pump Dry Instruments The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Dry Sequence of Steps The following is an overview for the sequence of steps mM A WN gt MOVE MOBILE RACK 1 Move Zto top 2 Move mobile rack DEC Zone over drain Move Mobile Rack on page D 23 Air Push on page D 24 Inside Rinse on page D 26 Outside Rinse on page D 26 Gas Air Purge on page D 26 TRILUTION LH Software Version 2 0 User s Guide D 23 Aig D
279. e Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Solution Zone The zone from which the Solution Volume is drawn Solution Well The well in the Solution Zone from which the Solution Volume is drawn 1 Solution The quantity of solution OuL Volume Solution AirGap The quantity of the air gap aspirated before the Solution Volume 20 uL Solution Flow The speed at which the Solution Volume moves into the probe For a range of 5 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Solution Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source and Solution Volumes are delivered SS Add Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide B 7 PPV SS SS Add Tasks SS Liquid Handling SS Add Properties Continued Property Name Brief Description Default Value Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered Result Flow Rate The speed at which the Source and Solution Volumes move out of the probe For 5 mL min a range of suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch O
280. e 44 Aluminum 2 Reitan Code 00 Retan Cade 0 Senes 2 K Rectan The Rack Elements Window 3 Enter the X Y and Z Relative positions The position is calculated with respect to the center of the rack When X 0 and Y 0 the center of the element is positioned over the center of the rack When Z 0 the bottom of the element is placed at the same level as the top of the rack level with the point of contact between the rack and its holder tray or antler 4 Optionally select a value from the Rotation drop down Default is 0 Rotation values 90 180 and 270 TRILUTION LH Software Version 2 0 User s Guide H 13 s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities 5 Click ADD Confirm that the element was added in the Preview 6 Click CLOSE to return to the Rack Adding multiple elements This option allows you to add an array of identical elements to a rack 1 Right click on a rack and then select ADD ELEMENTS 2 Select the element that you want to add as an array 3 Select the Multiple check box to enable the necessary fields 4 Enter the X Y and Z Relative positions for the center of the element in column 1 row 1 The position is calculated with respect to the rack center When X 0 and Y 0 the element in column 1 row 1 is centered over the center of the rack When Z 0 the bottom of the element is placed at the same level as the top of the rack level with
281. e B 26 SS Aliquot Instruments on page B 29 SS Aliquot Sequence of Steps on page B 30 B 20 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Aliquot Properties Property Name Brief Description Default Value Source Select Reservoir or Tray Reservoir Reservoir Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray The zone from which the Result Volume is drawn Source Zone Tray The well in the Source Zone from which the Result Volume is drawn 1 Source Well Tray Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray An additional volume to the Result Volume aspirated It ensures that the actual OuL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Result Volume Tray The quantity of the air gap aspirated before the Result Volume 20 uL Source Air Gap Tray The speed at which the Result Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate SS Aliquot Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide B 21 yonbijy ss SS Aliquot Tasks SS Liquid Han
282. e Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Add Properties Table Page 2 of 4 TRILUTION LH Software Version 2 0 User s Guide A 7 ppv Add Tasks Liquid Handling Add Properties Continued A Property Name Brief Description Default Value Solution Zone The zone from which the Solution Volume is drawn Solution Well The well in the Solution Zone from which the Solution Volume is drawn When 1 using a multiple probe instrument type x where x is a value defined in the Sample List Solution The quantity of solution OuL Volume Solution Air Gap The quantity of the air gap aspirated before the Solution Volume 20 uL Solution Flow The speed at which the Solution Volume moves into the probe For a range of 5 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Solution Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source and
283. e The zone in which the mix is performed Mix Well The well in the Mix Zone in which the Mix is performed 1 Solvent Valve Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Position position designated for waste cannot be used Number of Number of times the Task should repeat the commands related to mixing 1 Mixes Mix with Air Mix Select Mix with Air or Mix with Liquid Mix with with Liquid Liquid Mix with Liquid The quantity of liquid used as part of a mixing process OuL Mix Volume Mix with Liquid The quantity of an air gap aspirated before the Mix Volume 20 uL Air Gap Mix with Liquid The speed at which the Mix Volume moves into and out of the probe Fora range 5 mL min Flow Rate of suggested flow rate values see Flow Rates on page 4 93 Mix with Liquid The speed at which the Air Gap Volume moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Mix with Liquid Time the program waits after each dispense before moving out of the well 0 min Equilibration Time SS Mix Properties Table Page 1 of 2 B 114 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Mix Properties Continued Property Name Brief Description Default Value Mix with Liquid Select Touch Off to move to the edge of the well after dispensing in the X Touch Off direction Mi
284. e Version 2 0 User s Guide Tasks Liquid Handling Prime Pump This task primes the pump in the specified zone It aspirates the specified volume or the syringe capacity from the reservoir and then dispenses to the wells in the result zone Properties Advanced Rtimsing Pestruments For more information about the properties on each tab see 6 Prime Pump Prime Pump Properties on page A 94 Prime Pump Advanced Rinsing on page A 94 Prime Pump Instruments on page A 96 Prime Pump Sequence of Steps on page A 96 TRILUTION LH Software Version 2 0 User s Guide A 93 duing awd Prime Pump Tasks Liquid Handling Prime Pump Properties A Property Name Brief Description Default Value Source Source for the Prime Pump is reservoir Reservoir Prime Volume The quantity of the liquid used for priming OuL Source Flow The speed at which the Prime Volume moves into the syringe For a range of 10 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Prime Volume is delivered Inside Rinse Result Well The well in the Result Zone to which the Prime Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Prime Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Prime Pump Prop
285. e Zone from which the Source Volume is drawn When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual OuL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Diluent Select Reservoir Tray or Transfer Port Tray Tray The zone from which the Diluent Volume is drawn Diluent Zone Tray The well in the Diluent Zone from which the Diluent Volume is drawn When using 1 Diluent Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of liquid used to dilute the Source Volume OuL Diluent Volume Dilute Properties Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Dilute Properties Continued A Property Name Brief Descr
286. e and replaces it in the Application Allows you to reload the Tasks palette with any new Tasks created It basically allows you to update the builder with the latest Tasks It also allows you to update any changes to the unit settings Refresh after deleting and before adding new instruments RUN Launches the Application Configuration Tab For details about this tab and how to create a Configuration see Chapter 4 Method Builder Configuration TRILUTION LH Software Version 2 0 User s Guide 3 3 Japjing pouaW Method Builder Method Builder 3 Bed Layout Tab For details about this tab and how to create a Bed Layout see Chapter 5 Method Builder Bed Layout Method Tab For details about this tab and how to add steps to a Method see Chapter 6 Method Builder Method Error Handling Tab For details about this tab see Chapter 7 Method Builder Error Handling 3 4 TRILUTION LH Software Version 2 0 User s Guide Method Builder Viewing a Method 1 On the Method Builder do one of the following a On the Methods palette do one of the following Double click a Method Right click on a Method and then on the submenu displayed click OPEN METHOD b Click OPEN The Open Method window displays The Open Method Window 2 Onthe Open Method window select a Method and click OK The Method displays in the workspace TRILUTION LH Software Version 2 0 User s Guide 3 5 pou
287. e is 0 Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Cherry Pick with Tips Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Cherry Pick with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Cherry Pick with Tips Advanced Rinsing Table Page 3 of 3 Cherry Pick with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Cherry Pick with Tips Instruments C 11 TRILUTION LH Software Version 2 0 User s Guide sd YIM 991d A1149 Cherry Pick with Tips Tasks Disposable Tips C Cherry Pick with Tips Sequence of Steps The following is an overview for the sequence of steps L
288. e number of 1s in the group of bits Even Even Odd None M 1 S 0 Bits per Character The number of binary digits that constitutes a 7 5 6 7 8 character Stop Bits The number of bits to be transmitted after each 1 1 2 character Handshake The device with which information is being None Hardware Software Both transmitted or exchanged 606 GSIOC to RS 232 Converter Properties Table Page 2 of 3 None TRILUTION LH Software Version 2 0 User s Guide 4 37 uoleinbiyuo e Gules Creating a Configuration Method Builder Configuration 606 GSIOC to RS 232 Converter Properties Continued Property Name Brief Description Default Value Range General Instrument Name The name that will be used to identify this Communication N A instrument in a Task GSIOC ID A unique numerical address that must be known 61 0 63 to the instrument and to the software that allows the software to communicate to the instrument 606 GSIOC to RS 232 Converter Properties Table Page 3 of 3 4 38 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 819 Injection Module 819 Injection Module Properties Window Properties 819 Injection Module Properties Property Name Brief Description Default Value Range Injection Module Name The name that will be used to identify this instrument in a Task Injector N A GSIOC ID A unique numerical address that must be known
289. e outside rinse position in the rinse station SS Derivatize Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide B 53 z ean d SS SS Derivatize Tasks SS Liquid Handling SS Derivatize Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Derivatize Outside Rinse Table Page 3 of
290. e position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off 7 Move Z to Z Safe Height Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station B 88 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dispense Random to Random This task transfers liquid source from wells in the source zone to random wells in the result zone TRILUTION LH Software Version 2 0 User s Guide For more information about the properties on each tab see SS Dispense Random to Random Properties on page B 90 SS Dispense Random to Random Advanced Inside Rinse on page B 91 SS Dispense Random to Random Advanced Outside Rinse on page B 94 SS Dispense Random to Random Instruments on page B 97 SS Dispense Random to Random Sequence of Steps on page B 98 B 89 wopuey 0 Wopuey asuadsiq SS SS Dispense Random to Random Tasks SS Liquid Handling SS Dispense Random to Random Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn 1 Solvent Valve Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Position position designated for waste cannot be used
291. e probe using liquid from the pump reservoir Properties Binstruments Inside Volume ul Inside Flow Rate mijeiny fio Ovtaide Volume ut 500 Outzide Flow Rate mi fmin r 110 Invide Rinse Height mm P OutsideRinsetteght mm 19 l al 3 Rinsing Parameters A Inside Rinse Zone p m i ti Qutzide Rinse Zone For more information about the properties on each tab see Rinse Probes Properties on page A 104 Rinse Probes Instruments on page A 105 Rinse Probes Sequence of Steps on page A 106 Rinse Probes Properties Property Name Brief Description Default Value Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse The zone to which the Outside Rinse Volume is delivered Outside Rinse Zone Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Rinse Probes Properties Table Page 1 of 2 A 104 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Rinse Probes Properties Continued A Property Name Brief Description Default Value Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the pr
292. e pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Wash Instruments D 108 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Wash Sequence of Steps The following is an overview for the sequence of steps N OW A U N gt Move Mobile Rack on page D 109 Aspirate on page D 109 Dispense on page D 111 Air Push on page D 113 Inside Rinse on page D 115 Outside Rinse on page D 115 Gas Air Purge on page D 115 MOVE MOBILE RACK 1 Move Z to top 2 Move mobile rack DEC Zone over drain ASPIRATE Reservoir 1 Move Z to top 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to DEC Well in DEC Zone 4 Lower probe to Result Height from Tube Top 5 Dispense Source Volume from Reservoir at Result Flow Rate 6 Wait Equilibration Time 7 Move Zto top TRILUTION LH Software Version 2 0 User s Guide D 109 ysem Wash Tasks SPE D If Source Volume is greater than Syringe Volume repeat steps 2 7 until Source Volume delivered 8 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top Tray Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 1 2 3 Lower probe into Sour
293. e speed at which the Source Volume and Extra Volume move into the probe For 6 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select the Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 6 mL min Source Flow Rate a range of suggested flow rate values see Flow Rates on page 4 93 Condition Properties Table Page 2 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Condition Properties Continued Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate val
294. e tray TRILUTION LH Software Version 2 0 User s Guide For more information about the properties on each tab see SS Aspirate Properties on page E 22 SS Aspirate Instruments on page E 22 SS Aspirate Sequence of Steps on page E 23 E 21 aze11dsy SS SS Aspirate Tasks Tweaks SS Aspirate Properties Property Name Brief Description Default Value Source Volume The quantity of a sample OuL Source Flow The speed at which the Source Volume and Extra Source Volume move into the 5 mL min Rate The speed at which the Source and Diluent Volumes move out of the probe Fora range of suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after the aspirate 0 min Time SS Aspirate Properties SS Aspirate Instruments Default Value Property Name Liquid Handler Brief Description The liquid handler that the Task will affect Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler SS Aspirate Instruments E 22 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks SS Aspirate Sequence of Steps 1 Aspirate Source Volume at Source Flow Rate 2 Wait Equilibration Time TRILUTION LH Software Version 2 0 User s Guide E 23 a e1ldsy SS SS Deliver Tasks Tweaks SS Deliver This Task dispenses liquid through the probe For more in
295. e well 0 min Equilibration Time Mix with Air Select Touch Off to move to the edge of the well after dispensing in the X Touch Off direction Mix Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Liquid Level Following Mix Advanced Rinsing Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option A Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom u
296. each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Transfer Properties Table Page 3 of 3 A 110 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Liquid Level Following Transfer Advanced Rinsing Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height Solution Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option A Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height Solution Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negat
297. ears This window displays the version of the Gilson GSIOC Utility the GSIOC32 DLL and the GSIOC Server or Driver and the Port IRQ and Baud set by the GSIOC Configuration Editor TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility K Commands Immediate Command Immediate commands request status information from an instrument These commands are executed immediately temporarily interrupting any command in progress Each command is a string of no more than 40 characters You can find a list of valid immediate commands for each instrument in its user s guide Buffered Command Buffered commands send instructions to an instrument These commands are executed one at a time Each command is a string of no more than 40 characters You can find a list of valid buffered commands for each instrument in its user s guide TRILUTION LH Software Version 2 0 User s Guide K 21 AINN DOISD GSIOC Utility GSIOC Utility K 22 Command Type Buffered Insert an Immediate Command There are two ways to insert an immediate command in Advanced mode Using the Edit menu 1 From the Unit ID drop down box select the unit ID of the instrument to send the command to Type a Command and Comment The comment is optional Select Insert Immediate from the Edit menu The new command will be inserted at the bottom of the command list Note The command will not be inserted if there is already a command line in the
298. eate New User window On the Create User window do the following a In the User Name field type a User name b In the User Description field optional type a brief description of the User c In the Password field type your password TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview d Inthe Confirm Password field type your password for confirmation User name should be unique e In the Select the Group section select the Group s the User is assigned to 4 Click OK TRILUTION LH Software Version 2 0 User s Guide 1 29 snual H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 To modify User properties 1 On the Users tab select a User The assigned Group name s and description s for that User display in the right frame 2 Click MODIFY The Modify User window displays The Modify User window 3 In the Modify User window you can do the following a In the User Description field optional change the description b In the Password field type a new password c In the Confirm Password field type the new password for confirmation 1 30 TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview 1 d Inthe Select the Group section select the check box to add the User to a group or clear the check box to remove the User from a Group e Click OK A User Name cannot be modified To delete a User 1
299. ected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User s Guide C 25 sdi y IM Wopuey 0 wopuey ssuadsig Dispense Random to Random with Tips Tasks Disposable Tips C b If Prewet Tip FALSE 1 Skip to step 5 5 Aspirate Source Volume at Source Flow Rate using Liquid Level Following if selected 6 Move Z to top if Extra Volume gt 0 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move to Source Well in Source Zone 3 Lower probe into well to Source Height 4 Prewet Tips only 1st Iteration a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if selected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected b If Prewet Tip FALSE 1 Skip to step 5 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 6 Move Z to top DISPENSE if Extra Volume 0 1 Move probe to Starting Well in Result Zone 2 Lower probe into well to Result Height 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected C 26 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips 4 Dispense Source Air Gap at Result Flow Rate 5 Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to ce
300. ected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Dispense Random to Random Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 93 wopuey 0 wopuey su ds q SS SS Dispense Random to Random Tasks SS
301. ed Property Name Brief Description UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position LOWER SYRINGE Descends the syringe s piston operating rod 25 of a full stroke as the syringe pump aspirates from the reservoir The valve will switch to the outlet position RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 215 Liquid Handler Manual Control Table Page 3 of 3 9 6 TRILUTION LH Software Version 2 0 User s Guide Applicatio n Manual Control 215 Liquid Handler without Pump a080cOe0D The 215 Liquid Handler without Pump Manual Control Window 215 Liquid Handler without Pump Manual Control Proper sated ices Brief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Click MOVE TO WELL Z Offset The distance up when a positive number is entered or down 2 mm when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right of the home 0 0 mm position Click MOVE X Y Y Enter a number to move to a position forward of the home 0 0 mm position Z Enter a number to move to an absolute Z value 0 0 mm Click MOVE Z 215 Liquid Han
302. ed Layout Tray Template GX 274 20 Series Racks GX 274 200 Series Racks G 24 GX 274 Liquid Handler and GX 274 ASPEC GX Rinse Solvent Station Footprint GX Rinse Solvent Station GX Rinse Solvent Station TRILUTION LH Software Version 2 0 User s Guide Rinse Locations GX Rinse Solvent Riser Rack GX Rinse Solvent Riser GX Rinse Solvent Riser Flow Through Rinse Locations Top Wells Rinse Station GX Rack pa Inside Rinse Locations Bottom Wells TRILUTION LH Software Version 2 0 User s Guide G 25 suol e907 asuly Rinse Locations Rinse Locations Flow Through Rinse Locations Top Wells Rinse Station GX 56mm Probes Inside Rinse Locations Bottom Wells G 26 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Solvent GX Rack Flow Through Rinse Locations Inside Rinse Locations Solvent Transfer Ports Top Wells Second Row of Wells from Top Bottom Two Rows of Wells Rinse Solvent GX 56mm Probes Rack Inside Rinse Locations Solvent Transfer Ports Top Wells Second Row of Wells from Top Bottom Two Rows of Wells Flow Through Rinse Locations TRILUTION LH Software Version 2 0 User s Guide G 27 suol e907 asuly Rinse Locations Rinse Locations Bed Layout Tray Template GX 281 G 28 GX 281 Liquid Handler GX Rinse Station Footprint ae tig GX 28
303. ed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 1 min Equilibration Time Elute Properties Table Page 5 of 5 Elute Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated This Following selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tubes This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Elute Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide D 33 any Elute Tasks SPE D Elute Advanced Rinsing Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unles
304. ed when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Cherry Pick Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide B 39 Id Y SS SS Cherry Pick Tasks SS Liquid Handling SS Cherry Pick Outside Ri
305. efault Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Add Instruments TRILUTION LH Software Version 2 0 User s Guide PPV SS SS Add Tasks SS Liquid Handling B SS Add Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 16 2 Dispense on page B 17 3 Inside Rinse on page B 18 4 Outside Rinse on page B 18 ASPIRATE Reservoir 1 Move Z to Z Safe Height 2 Move probe to Solution Well in Solution Zone 3 Aspirate Solution Air Gap at Air Gap Flow Rate 4 Lower probe into well to Solution Height 5 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 6 Move Z to Z Safe Height Tray Move Z to Z Safe Height Move probe to Source Well in Source Zone 1 2 3 Aspirate Source Air Gap at Air Gap Flow Rate 4 Lower probe into well to Source Height 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected B 16 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling CON OF 9 Move Z to Z Safe Height Move probe to Solution Well in Solution Zone Aspirate Solution Air Gap at Air Gap Flow Rate Lower probe into well to Solution Height 10 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Follo
306. efault Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Mix Advanced Inside Rinse Table Page 3 of 3 Omm B 118 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Mix Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the outside rinse position in the rinse station SS Mix Outside Rinse Table Page 1 of 4 10 mL min TRILUTION LH Software Version 2 0 User s Guide B 119 XIW SS SS Mix Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Mix Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Place
307. el Following if selected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User s Guide C 39 sdi y m asuadsig Dispense with Tips Tasks Disposable Tips C b If Prewet Tip FALSE 1 Skip to step 5 6 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 7 Move Z to top DISPENSE Reservoir Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected 2 If Source Volume is greater than tip volume repeat Aspirate step 4 and Dispense step 1 until Source Volume is dispensed 3 Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top C 40 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Tray if Extra Volume 0 Move probe to Result Well in Result Zone Lower probe into well to Result Height Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Dispense Source Air Gap at Result Flow Rate Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If
308. en closed off 3 on by selecting and clearing the boxes Output Contacts 4 A filled box indicates that the circuit is closed on An empty Toggle the contacts 5 box indicates that the circuit is open off open closed off 6 on by selecting and 7 clearing the boxes 8 Input Contacts 1 A filled box indicates that the circuit is closed An empty box Monitor status 2 indicates that the circuit is open 3 Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration 221 XL Liquid Handler Manual Control Table Page 2 of 3 9 12 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 221 XL Liquid Handler Manual Control Continued Property Brief Description Wane ief Descriptio UNLOCK ARM Unpowers the X Y and Z motors Default Value Use LOCK ARM Powers all motors and moves the probe to the home position 221 XL Liquid Handler Manual Control Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 222 XL Liquid Handler The 222 XL Liquid Handler Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 222 XL Liquid Handler Manual Contr
309. en using this option Source Height The distance up when a positive number is entered or down when a negative 2 mm number is entered from a defined reference point Tube Bottom unless Liquid Level Following is selected when aspirating the Source Volume Cherry Pick with Tips Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide C 9 sd YIM 991d A1149 Cherry Pick with Tips Tasks Disposable Tips Cherry Pick with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No rinse is performed 0 uL when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe No rinse is performed 0 uL when the Outside Volum
310. er than Transfer Tubing volume repeat Aspirate Air Gap and Extra Volume not aspirated and Dispense until Source Volume delivered 8 Move probe to Inside Rinse Well in Inside Rinse Zone 9 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 10 Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset Switch solvent valve to Inside Rinse Solvent Valve Position mM A U N gt Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position OM A WN Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume TRILUTION LH Software Version 2 0 User s Guide B 99 wopuey 0 wopuey su ds q SS SS Dispense Random to Random Tasks SS Liquid Handling B 6 Switch Peri
311. eries racks SPE tasks can be used on the following instruments e 215 Liquid Handler 215 Liquid Handler without Pump e 215 SW Liquid Handler e Multiple Probe 215 Liquid Handler e Quad Z 215 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D SPE SPE Tasks Gilson Task Name and Description Condition This task prepares the DEC to accept a prepared sample by aspirating then dispensing liquid source from the reservoir tray or transfer ports with the mobile rack positioned over the drain Dry This Task dries the DECs by pushing pressurized gas or air from the syringes through the DECs with the mobile rack positioned over the drain Elute This task aspirates the specified volume of liquid source from the reservoir tray or transfer ports and then dispenses the volume to the wells of the DEC zone with the mobile rack over the collect position Evaporate This task evaporates liquids in the collect wells by passing pressurized gas or air from the syringes into the wells with the mobile rack positioned over the drain SPE Tasks Table Page 1 of 2 D 2 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D SPE Tasks Continued Fractionate If multiple analytes of interest will be collected use this task to aspirate the specified volume from the reservoir tray or transfer ports and then dispense it to the DEC with the mobile rack positioned over the specified
312. ersion 2 0 User s Guide D 31 ain y Elute Tasks SPE D Elute Properties Continued Property Name Brief Description Default Value Solenoid Time the program waits after a solenoid air push before moving out of the well 0 1 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 20 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 6 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of the probe Fora range of 6 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 1 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Elute Properties Table Page 4 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Elute Properties Continued Property Name Brief Description Default Value Valve The length of time the air is dispens
313. erties Prime Pump Advanced Rinsing Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the tube bottom when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Prime Pump Advanced Rinsing Table Page 1 of 2 Outside Rinse TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Prime Pump Advanced Rinsing Continued A Property Name Brief Description Default Value Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and intothe 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing
314. erties Table Page 4 of 4 4 54 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 271 Liquid Handler without Pump Not Installed The GX 271 Liquid Handler without Pump Properties Window TRILUTION LH Software Version 2 0 User s Guide 4 55 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration Properties GX 271 Liquid Handler without Pump Properties Property Name Brief Description Default Value Range Setup Probe Type mm If the stop pin was inserted in S1 175 mm Probe 56 mm Probe select 56 mm Probe 125 mm Probe If the stop pin was inserted in S2 173 mm Prone select 125 mm Probe If no stop pin was used select 175 mm Probe Z Clamp Height mm This number corresponds to where 175 Calculated based on the mounting clamp is on the Z Clamp Height and Probe vertical ruler on the installed Z arm Type Range provided on screen Z Safe Height mm The Z height to which the 172 Calculated based on instrument s probe moves before Z Clamp Height and Probe moving in the X or Y direction Type Range provided on screen GX Direct Inject Valve Indicate the size of the valve Not Installed Not Installed installed 1 8 1 16 GX 271 Liquid Handler without Pump Properties Table Page 1 of 3 4 56 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 271 Liquid Han
315. ervoir N A liquid that is flowing through the associated syringe It is important for sample tracking Contacts Relay Output 1 Optionally type a description for the N A N A Relay Output 2 Relay Output 3 Output 4 Output 5 Output 6 Output 7 Output 8 contact This description will be used to identify the contact in a Task ASPEC XL4 Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 43 uoleinbyuos e Gules Creating a Configuration Method Builder Configuration ASPEC XL4 Properties Continued Property Name Brief Description Default Value Range Analog Input Optionally type a description for the N A N A Input 1 contact This description will be used to Input 2 identify the contact in a Task Input 3 Input 4 General Instrument Name The name that will be used to identify Liquid Handler N A this instrument in a Task GSIOC ID A unique numerical address that must 10 0 31 be known to the instrument and to the software that allows the software to communicate to the instrument ASPEC XL4 Properties Table Page 3 of 3 4 44 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration ASPEC XLi ASPEC XLi Properties Window Properties ASPEC XLi Properties Property Name Brief Description Default Value Range Setup Z Safe Height mm The Z height to which the instrument s probe moves before 0 183 to 0
316. es Continued Property Name Brief Description Default Value Source Flow The speed at which the Source Volume moves into the probe For a range of 5 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after the aspirate 0 min Time Aspirate with Tips Properties Table Page 2 of 2 Aspirate with Tips Tips Property Name Brief Description Default Value Prewet Tip Select this option to wet the tip prior to aspirating the Source Volume Prewet Volume Volume aspirated and dispensed to prewet the tip when Prewet Tip has been 10 uL selected Aspirate with Tips Tips Aspirate with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Aspirate with Tips Instruments E 8 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Aspirate with Tips Sequence of Steps 1 If Wet Tip TRUE a Aspirate Prewet Volume at Source Flow Rate b Dispense Prewet Volume at Source Flow Rate If Wet Tip FALSE a Skip to step 2 2 Aspirate Source Volume at Source Flow Rate 3 Wait Equilibration Time TRILUTION LH Software Version 2 0 User s Guide E 9 sdi YUM ajzesidsy Deliver Tasks Tweaks Deliver This task dispenses
317. es do one of the following e To save changes to the existing Task click SAVE i Gilson supplied tasks cannot be saved using the supplied name 2 12 TRILUTION LH Software Version 2 0 User s Guide Task Builder e To save changes to a new Task and save with a different name click SAVE AS The Save Task As window displays e On the Save Task As window do the following 1 In the Name field type a new Task name 2 In the Description field optional type a brief description about the Task Special characters and punctuation are not permitted in names or descriptions 3 Use the browse button to assign a custom icon 4 Click OK TRILUTION LH Software Version 2 0 User s Guide ysey e Bulsyipow Saving a Task Task Builder 2 Saving a Task 1 Click SAVE The Save Task window appears Arini trator Adcniustrator Achomstrator Administrator Administrator Achnintitratoe Administrator Administrator The Save Task window 2 On the Save Task window do the following a In the Name field type a unique Task name b In the Description field optional type a brief description of the Task c In the Custom Icon field optional use the browse button to assign a custom icon TRILUTION LH Software Version 2 0 User s Guide Task Builder Exporting Tasks 1 On the Task Builder click EXPORT The Select items to Export window displays EJ Select items to Export Select the ite
318. escription Default Value Range Contacts 24V Output Optionally type a description for the contact This N A N A Output 1 description will be used to identify the contactina Output 2 Task Output 3 Output 4 Input A Optionally type a description for the contact This N A N A Input B description will be used to identify the contactina Input C Task Input D General Instrument Name The name that will be used to identify this instrument in a Task Liquid Handler N A GSIOC ID A unique numerical address that must be known to the instrument and to the software that allows the software to communicate with the instrument 215 SW Liquid Handler Properties Table Page 2 of 2 22 20 29 4 14 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 221 XL Liquid Handler The 221 XL Liquid Handler Properties Window Properties 221 XL Liquid Handler Properties Property Name Brief Description Default Value Range Setup Z Arm Type mm This is the height of the Z arm installed onthe 123 mm Z Arm 56 mm Z Arm 123 mm Z Arm liquid handler Z Safe Height mm The Z height to which the instrument s probe 2 Calculated based on Z Clamp moves before moving in the X or Y direction 221 XL Liquid Handler Properties Table Page 1 of 2 Height and Z Arm Type Range provided on screen TRILUTION LH Software Version 2 0 User s Guide 4 15
319. eservoir fluid moves into and out of the syringe Buttons HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position LOWER SYRINGE Descends the syringe s piston operating rod 25 of a full stroke Used when replacing a syringe RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 406 Pump Single Manual Control TRILUTION LH Software Version 2 0 User s Guide 9 27 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Property Name Active Syringes Manual Control 9 444 QuadDilutor The 444 QuadDilutor Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 444 QuadDilutor Manual Control Brief Description Default Value Use The syringes that will be used A B C D Click PRIME to start or STOP PRIME Prime Flow Rate The speed at which the reservoir fluid moves 10 mL min into and out of the syringes to end Buttons HOME SYRINGE Homes the dilutor and sends the piston s to the upper position with the valve s in the probe position 444 QuadDilutor Manual Control Table Page 1 of 2 9 28 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 444 QuadDilutor Manual Control Contin
320. esult Zone Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone SS Dilute Advanced Inside Rinse Table Page 2 of 4 TRILUTION LH Software Version 2 0 User s Guide B 65 7N Ad SS SS Dilute Tasks SS Liquid Handling SS Dilute Advanced Inside Rinse Continued Property Name Brief Description Default Value Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Dilute Advanced Inside Rinse Table Page 3 of 4 B 66 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Dilute Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Place
321. ethod Builder 3 12 3 On completion of the Import operation the TRILUTION information box displays B Runon Q Import operation is corr more nformation The TRILUTION information dialog box c On the TRILUTION information dialog box complete the following e To view the log information of the Import operation click DETAILS e Click OK TRILUTION LH Software Version 2 0 User s Guide Method Builder Deleting Methods 1 On the Methods palette do one of the following Select a Method and then right click to view the submenu On the submenu click DELETE e Open a Method and on the action bar click DELETE A Delete confirmation dialog box displays IRILU TION Are you Method BB Delete 4 ersions The Delete confirmation dialog box On the Delete confirmation dialog box do one of the following e Click yes The Method is deleted Select the Delete All Versions check box and then click Yes All versions of the Method are deleted TRILUTION LH Software Version 2 0 User s Guide spoy aw buynajaq Closing the Method Builder Method Builder 3 Closing the Method Builder On the Method Builder do one of the following Click on the lower right corner of the Method Builder to exit and return to the TRILUTION menu Click E on the upper right corner of the Method Builder to close the builder 3 14 TRILUTION LH Software Version 2 0 User s Guide Method Builder Co
322. f 3 TRILUTION LH Software Version 2 0 User s Guide C 33 sdi y m asuadsig Dispense with Tips Tasks Disposable Tips Dispense with Tips Tips Continued Property Name Brief Description Default Value Eject Tips After Select and all tips will be ejected before the rinses Active Task Clear and the tips will remain loaded Prewet Tip Select this option to wet the tip before aspirating the Source Volume 1st Iteration Cleared only Prewet Volume The quantity of liquid source aspirated and dispensed to prewet the tip when 10 uL Prewet Tip has been selected Touch Off Mode Used when Touch Off was selected on the Properties tab select Side or Surface Surface touch off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Surface number is entered from an auto calculated position to which the tip will move Z Offset when performing a surface Touch Off Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Touch Off The distance right when a positive number is entered or left when a negative 0 mm Mode Side number is entered from the edge of the tube that the tip will move when X Offset performing a side Touch Off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Side number is entered from the Tube
323. f a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Add Properties Table Page 1 of 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Add Properties Continued A Property Name Brief Description Default Value Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone designated for the transfer ports from which the Source Volume is Source Zone drawn Transfer Port The transfer port in the Source Zone from which the Source Volume is drawn A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and th
324. f the Disposable Tips Tasks Tasks SPE Provides a list and description of the SPE Tasks Tasks Tweaks Provides a list and description of the Tweaks Tasks Tasks Auxiliary Provides a list and description of the Auxiliary Tasks Rinse Locations Provides a description of rinse positions Bed Layout and Utilities Provides information about building custom racks and templates for use in Bed Layouts Electronic Record Management ERM Features Provides information about the features available in TRILUTION LH to assist in attaining 21 CFR Part 11 compliance through implementation of Electronic Record Management ERM GSIOC Configuration Editor Provides information about how the GSIOC Configuration Editor enables you to modify COM serial communications port and baud rate information GSIOC Utility Provides information about how to use the GSIOC Utility to issue Commands to Gilson GSIOC instruments Glossary Terminology Definition Application A file with a set of Methods to execute a set of actions to obtain the experiment results Application Run The act of executing an Application to get desired results Bed Layout A file that specifies the location of solutions on the bed and rinse stations Command A Control call representing a single action Configuration A group of selected instruments Element A base record that includes foot
325. ff Transfer with Tips Tips Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Transfer with Tips Tips Continued Property Name Brief Description Default Value Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Transfer with Tips Tips Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide C 81 sdi YUM JajsuerlL Transfer with Tips Tasks Disposable Tips Transfer with Tips Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will
326. ff Select Touch Off to move to the edge of the well after dispensing in the X direction SS Add Properties Table Page 3 of 3 B 8 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Liquid Level Following SS Add Advanced Inside Rinse Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height Solution Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height Solution Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following i
327. ffset 3 Switch Peristaltic Rinse Pump on at Rinse Pump Speed TRILUTION LH Software Version 2 0 User s Guide B 43 Id D SS SS Cherry Pick Tasks SS Liquid Handling B 4 Switch solvent valve to Rinse Well 1 Solvent Valve Position 5 Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off 7 Move Zto Z Safe Height Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station B 44 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B SS Derivatize This task dispenses the specified volumes of liquid source from the reservoir or tray and solution from the tray to the wells of the result zone and then mixes with liquid or air Properties Advanced inide Rinse Outside tise ftmstruments For more information about the properties on each tab see o SS Derivatize Properties on page B 46 SS Derivatize Advanced Inside Rinse on page B 49 SS Derivatize Outside Rinse on page B 52 SS Derivatize Instruments on page B 55 SS Derivatize Sequence of Steps on page B 56 TRILUTION LH Software Version 2 0 User s Guide B 45 9ZILALIIG SS SS Derivatize Tasks SS Liquid Handling SS Derivatize Properties Property Name
328. figuration can include one or more VPS Syringe VALYEMATE 4 Pump B Pump A Pump 4 B Pump 4 alve None None None The Virtual Pumping System Properties Window 4 88 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Properties Virtual Pumping System hes Brief Description Default Value Setup Probe The probes in the Configuration using the VPS Syringe The syringes in the Configuration using the VPS VALVEMATE The VALVEMATE that the VPS will use if any Position The valve position A H on the VALVEMATE Required when a VALVEMATE is selected General Pump Name The name that will be used to identify this instrument in a Task Pump Virtual Pumping System TRILUTION LH Software Version 2 0 User s Guide 4 89 uoleinbyuo e Burze Creating a Configuration Method Builder Configuration 4 90 Using a VPS To Use Multiple Reservoir Solutions For a system using multiple reservoir solutions complete the following 1 Drag and drop a single probe liquid handler with installed single syringe pump into the workspace for example 215 Liquid Handler or Micro 215 and set the properties Drag and drop a VALVEMATE into the workspace and set the properties Your VALVEMATE should be plumbed and ready to go at this point Drag and drop a Virtual Pumping System into the workspace a In the VALVEMATE field click the arrow and then select
329. flow rates refer to Tubing Options and Associated Maximum Flow Rates on page 4 72 GX Analytical Solvent System The suggested minimum flow rate is 0 001 mL min The suggested maximum flow rate is 5 mL min GX Prep Solvent System The suggested minimum flow rate is 0 001 mL min The suggested maximum flow rate is 50 mL min 4 94 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Modifying a Configuration Modify a Configuration by doing one or more of the following e Adding new instruments to an existing Configuration e Deleting instruments from an existing Configuration e Modifying the instrument properties of the instruments in a Configuration When you right click on an instrument in the Workspace a menu displays with the option to DELETE the instrument You can delete selected instruments in the workspace by pressing the DELETE key Clear Default When a Method is saved the Configuration is named An option is provided to set that Configuration and its associated Bed Layout as a default for future new Methods Click CLEAR DEFAULT to remove a Configuration and its associated Bed Layout as default TRILUTION LH Software Version 2 0 User s Guide 4 95 uoieinbiyuo gt e bulfjipo Method Builder Bed Layout A Bed Layout serves as one of the building blocks of a Method and consists of the following Template Footprints Rack s Well s TRILUTION LH Software Versi
330. follow the liquid down as it is aspirated and up Active Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Source Height The distance up when a positive number is entered or down when a negative 2 mm number is entered from a defined reference point Tube Bottom unless Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No rinse is performed O uL when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe No rinse is performed 0 uL when the Outside Volume is 0 Transfer with Tips Advanced Rinsing
331. formation about the properties on each tab see Properties Binstruments i p _ SS Deliver Properties on page E 24 lias t SS Deliver Instruments on page E 25 Solvent Valve Position 3 x s Resuttvolume ay f SS Deliver Sequence of Steps on page E 26 Result Raw Rare mi fein i Eguilibration Timema fo OK Cancel aip SS Deliver Properties Property Name Brief Description Default Value Solvent Valve Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Position position designated for waste cannot be used Result Volume The quantity of liquid delivered O uL SS Deliver Properties Table Page 1 of 2 E 24 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Property Name Result Flow Rate SS Deliver Properties Continued Brief Description The speed at which the Result Volume moves out of the probe For a range of suggested flow rate values see Flow Rates on page 4 93 Default Value 5 mL min Equilibration Time Time the program waits after the dispense SS Deliver Properties Table Page 2 of 2 0 min Property Name Liquid Handler SS Deliver Instruments Brief Description The liquid handler that the Task will affect Default Value Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler SS Deliver Instruments
332. from the Info Window during the run in Microsoft Notepad EXPORT Exports a copy of the Results to a specified location as a LHRE file To select multiple run results in a sequential order hold SHIFT any arrow key simultaneously To select run results in a random order hold CTRL and select multiple run results with the mouse ARCHIVE Moves the Results to a specified location as a LLHRE file To select multiple run results in a sequential order hold SHIFT any arrow key simultaneously To select run results in a random order hold CTRL and select multiple run results with the mouse RESTORE Imports an exported or archived LHRE file and lists the Result in the Run Results window REFRESH Reloads the window with any new or imported run results TRILUTION LH Software Version 2 0 User s Guide 8 21 Run Results Window Application Result Double click the run name to show the result Right click Menu Run Results Menu VIEW LOG RENAME RUN EXPORT RESULT ARCHIVE RESULT RESTORE RESULT 8 22 Description Opens the log file detailing information from the Info Window during the run in Microsoft Notepad Assign a different name to a run Exports a copy of the Results to a specified location as a LHRE file Moves the Results to a specified location as a LHRE file Imports a LHRE file and lists the Result in the Run Results window TRILUTION LH Software Version 2 0
333. g if selected 6 Move Z to top DISPENSE if Extra Volume 0 1 Move probe to Starting Well in Result Zone 2 Lower probe into well to Result Height 3 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User s Guide sd YIM 991d A1149 Cherry Pick with Tips Tasks Disposable Tips C 4 Dispense Source Air Gap at Result Flow Rate 5 Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 7 If Source Volume is greater than tip volume repeat aspirate and dispense until Source Volume is delivered if Extra Volume gt 0 Move probe to Starting Well in Result Zone Lower probe into well to Result Height Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well U A U N C 14 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 6 If Source Volume is greater than tip volume repeat aspirate Air Gap and Extra V
334. g On Window TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Both Operating Systems In the TRILUTION log on window do the following e Inthe User Name field type your user name Inthe Password field type your password Password is case sensitive and has a 20 character limit e Click LOG ON The Liquid Handling menu displays MTT EVENT TTT eo T N S TRILUTION LH SOFTWARE i LIQUID HANDLING Application Mathod Builder Custom Task Bullder Utilittes s CKU Handling TRILUTION LH Software Version 2 0 User s Guide 1 9 H7 NOILNTIYL BuIypuNeT Help TRILUTION LH Software Overview 1 Help On line help is included with TRILUTION LH The on line help describes commands and dialogs displayed in the software and describes the procedures needed to perform tasks Access the on line help using the following e Windows 2000 Click START and then select PROGRAMS gt GILSON APPLICATIONS gt TRILUTION LH gt TRILUTION LH HELP Windows XP Click START and then select ALL PROGRAMS gt GILSON APPLICATIONS gt TRILUTION LH gt TRILUTION LH HELP e Place your mouse cursor over a button within TRILUTION LH to view the pop up description for that button e Click D to display help about the dialog box e Click to display help about the window Click to display help about the menu 1 10 TRILUTION LH Software Version 2 0 User s Guide
335. g Table Page 3 of 3 Derivatize Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Derivatize Instruments A 39 TRILUTION LH Software Version 2 0 User s Guide aZNeALAG Derivatize Tasks Liquid Handling A Derivatize Sequence of Steps The following is an overview for the sequence of steps Aspirate on page A 40 Dispense on page A 42 Mix with Liquid on page A 42 Mix with Air on page A 43 Inside Rinse on page A 43 Nn A U N gt Outside Rinse on page A 44 ASPIRATE Reservoir Move Z to Z Safe Height Aspirate Source Volume from Reservoir at Source Flow Rate Move probe to Solution Well in Solution Zone Aspirate Solution Air Gap at Air Gap Flow Rate Lower probe into well to Solution Height Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height N OAU A U N gt Tray 1 Move Z to Z Safe Height 2 Move probe to Source Well in Source Zone A 40 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling CON WN HR W 9 Aspirate Source Air Gap at Air Gap Flow Rate Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Mo
336. g on the Repeat field and selecting the Auto Repeat check box Starting from the top commandline this mode will refresh one repeating command line every 1 10th of a second For example if there are ten command lines with Repeat selected each of these command lines will be initiated every second Auto Repeat mode is useful for monitoring the instrument using immediate commands and is designated in the Repeat field with a green check mark Auto Repeat mode is not intended to be used as a programming tool with buffered commands Repeating buffered commands will be executed ata fixed time interval regardless if a command has finished For this reason the check mark in the buffered command line is yellow Auto Repeat mode can be selected from the Mode menu or by selecting the check box next to Auto Repeat Auto Repeat is deselected by default with the Advanced mode is first opened When Gilson GSIOC Utility Advanced is saved or closed the status of Auto Repeat is saved TRILUTION LH Software Version 2 0 User s Guide AINN DOISD GSIOC Utility GSIOC Utility K 20 Ghost The Ghost option in the Mode menu makes the GSIOC Utility window transparent and always on top This allows you to view another window behind the Utility The Utility will remain fully functional in the Ghost mode Help Menu Help Topics When selected the Help window for the Gilson GSIOC Utility appears About When selected the About window app
337. ge 2 of 2 4 22 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 402 Pump Dual with Tee The 402 Dual with Tee is a uniquely designed syringe pump that allows for two different syringe sizes to function as one syringe pump For the syringe pump to function properly the following will be true The left syringe capacity is greater than the right syringe capacity The following describes how the syringes work together ASPIRATE TRAY The valve must be in the probe position The right syringe will be used if the requested volume is less than 95 of the right syringe capacity Held volume is considered The left syringe will be used if the requested volume is 95 or greater than the right syringe capacity ASPIRATE RESERVOIR The valve must be in the reservoir position The left syringe will be used TRILUTION LH Software Version 2 0 User s Guide 4 23 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration 4 DISPENSE e The right syringe will be used if the requested volume is less than 95 of the right syringe capacity Held volume is considered e The left syringe will be used if the requested volume is 95 or greater than the right syringe capacity ID Syringe Name Size pL Reservoir Name i gt A A 10000 Reservoir f The 402 Pump Dual with Tee Properties Window Properties 402 Pump Dual with Tee Properties are Default
338. ge 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 29 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration 406 Pump Single hel iH ID Syringe Name Size pL Reservoir Name gt A A 1000 Reservoir The 406 Pump Single Properties Window Properties 406 Pump Single Properties ee Default Property Name Brief Description Value Range Setup ID A unique identifier to address syringe on the A A instrument Syringe Name A unique name to address syringe on the A N A instrument 406 Pump Single Properties Table Page 1 of 2 4 30 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 406 Pump Single Properties Continued se a Default Property Name Brief Description Value Range Size uL The capacity of each installed syringe For a range 10000 100 250 500 1000 5000 of suggested flow rates for this syringe size see 10000 25000 Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid thatis Reservoi N A flowing through the associated syringe It is r important for sample tracking General Instrument Name The name that will be used to identify this Pump N A instrument in a Task Pump Serial Number The serial number of the pump The serial number N A N A is located on the rear panel and near the bottom of the instrument 406 Pump Single Properties Table Page
339. ge C 84 Aspirate on page C 84 Dispense on page C 86 Eject Tips Optional on page C 88 Inside Rinse Optional on page C 89 Outside Rinse Optional on page C 89 Rinse Touch Off on page C 89 AD TIPS OPTIONAL Move Z to top If Load Tips before Task TRUE load tips PIRATE Reservoir 1 2 3 4 C 84 Move Z to top Move probe to Result Well in Result Zone Lower probe into well to Result Height Aspirate Source Volume from Reservoir at Source Flow Rate TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Tray if Extra Volume 0 1 2 3 4 Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Prewet Tips only 1st Iteration a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if selected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected b If Prewet Tip FALSE 1 Skip to step 5 Aspirate Source Volume at Source Flow Rate using Liquid Level Following if selected Move Z to top Tray if Extra Volume gt 0 U A U N Move Z to top Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Prewet Tips only 1st Iteration a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if selected 2 Dispense Prewet Volume a
340. ght from Tube Bottom 9 Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top TRILUTION LH Software Version 2 0 User s Guide D 93 33 0 gt pue peo Load and Collect Tasks SPE Transfer Port Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time mM A WN gt Move Z to top Dispense until Source Volume delivered 6 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top 7 Move probe to well in Inside Rinse Zone 8 Lower probe to Inside Rinse Height from Tube Bottom 9 Dispense Extra Volume Air Gap at Result Flow Rate 10 Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume not aspirated and TRILUTION LH Software Version 2 0 User s Guide Tasks SPE AIR PUSH Solenoid 1 2 3 Oo ON WD MN RA Move probe to well in Inside Rinse Zone Aspirate 250 uL at 5 mL min Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output
341. hat command line Unit This is the Unit ID for the instrument in the command line Type Immediate Command B Buffered Command Command This is the GSIOC command for the command line TRILUTION LH Software Version 2 0 User s Guide AINN DOISD GSIOC Utility GSIOC Utility Response This is the GSIOC response when the command line is initiated with a double click on the Unit Type Command Response or Comment field The response will become gray after 15 seconds if no response is registered Comment This is an optional comment that can be added to the command line Advanced Mode Menus File Menu There are four options in the File menu Open Save Save As and Exit Open The Open option in the File menu allows you to open previously created command lists GSUTIL32 files The extension for a GSUTIL32 file is GSU TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility Save The save option in the File menu allows you to save the Advanced commands to GSUTIL32 GSU The GSUTIL32 GSU file is automatically created in the location where GSUTIL32 EXE is stored The Advanced commands will automatically be saved to GSUTIL32 GSU if the Gilson GSIOC Utility is exited without saving The GSUTIL32 GSU is automatically opened when the Advanced mode is selected from the Mode menu Save As The Save As option on the File menu allows you to save the Gilson GSIOC Utility commands that are cu
342. he Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position GX 271 ASPEC without Pump Manual Control Table Page 3 of 3 Toggle the solenoids off on by selecting and clearing the boxes Click SWITCH TRILUTION LH Software Version 2 0 User s Guide 9 45 MOPpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 9 GX 271 Liquid Handler The GX 271 Liquid Handler Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons GX 271 Liquid Handler Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number 2mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom GX 271 Liquid Handler Manual Control Table Page 1 of 4 9 46 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 GX 271 Liquid Handler Manual Control Continued Property Name Brief Description Default Value Use X Enter a number to move to a position to 0 0 mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0
343. he Sample List 1 2 3 4 Open the Application window Place the cursor in the first cell for which you will be selecting a well Click to select the Bed Layout View tab Select a Zone from the drop down list Available wells appear highlighted in the active zone color Choose multiple well locations as described following TRILUTION LH Software Version 2 0 User s Guide Application 8 To select random wells within one zone a Press the CTRL key and select each well To b Click SEQUENTIAL SINGLE SEQUENTIAL MULTIPLE Or BATCH 9 1 If SEQUENTIAL SINGLE all wells selected will be added as one row o in the Sample List and the mode will be set to Sequential For more information on sequential processing see Sequential on page 8 17 2 If SEQUENTIAL MULTIPLE each well selected will be added as its own row in the Sample List and the mode will be set to Sequential For more information on sequential processing see Sequential on page 8 17 3 If BATCH all wells selected will be added as one row in the Sample List and the mode will be set to Batch For more information on batch processing see Batch on page 8 17 TRILUTION LH Software Version 2 0 User s Guide 8 9 Application Window Application 8 To select a range of wells within one zone a Lasso the range of wells b Click SEQUENTIAL SINGLE SEQUENTIAL MULTIPLE Or BATCH 1 If SEQUENTIAL SINGLE all wells selected will be added as one row in the Samp
344. he Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Inject Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 105 p ful ss SS Inject Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Inject Advanced Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Inject Advanced Outside
345. he circuit is closed An B empty box indicates that the circuit is open 506C System Interface Manual Control Table Page 2 of 2 Default Use Value Monitor status TRILUTION LH Software Version 2 0 User s Guide 9 31 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control 9 819 Injection Module a a 819 Injection Module Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 819 Injection Module Manual Control Brief Description Default Value Use Rotates the valve to the selected position Click SWITCH 819 Injection Module Manual Control Property Name Valve Position 9 32 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control ASPEC XL The ASPEC XL Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons ASPEC XL Manual Control Propert l PEPE pery Brief Description Default Value Use Name Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number is 2 mm Click MOVE TO WELL entered or down when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right 0 0 mm of the home posit
346. he proper RS 232 or GSIOC connection exists between the computer and the instruments and that the instruments do not have duplicate Unit ID numbers Advanced The advanced option in the Mode menu allows you to switch between the Basic and Advanced modes A check mark means that the GSIOC Utility is in Advanced mode Ghost The Ghost option in the Mode menu makes the GSIOC Utility window transparent and always on top This allows you to view another window behind the Utility The Utility will remain fully functional in the Ghost mode TRILUTION LH Software Version 2 0 User s Guide Unit ID Scan Auto Repeat Ghost K 7 AINN DOISD GSIOC Utility GSIOC Utility K 8 Help Menu Help Topics When selected the Help window for the Gilson GSIOC Utility appears About When selected the About window appears This window displays the version of the Gilson GSIOC Utility the GSIOC32 DLL and the GSIOC Server or Driver and the Port IRQ and Baud set by the GSIOC Configuration Editor TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility K Advanced Mode In the Advanced mode immediate and buffered GSIOC commands can be sent to specific Gilson instruments In this mode immediate and buffered commands with comments can be saved to a command list to be used as needed The command lines can be repeated automatically to monitor the status of the instrument AINN DOISD Tt Gilson GSIOC Utilit
347. hich support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Mix Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Mix Zone SS Mix Advanced Inside Rinse Table Page 1 of 3 2mm B 116 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Mix Advanced Inside Rinse Continued Property Name Brief Description Default Value Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid use
348. ht Rinse Station 223 Right Rinse Station 223 Short Right Rack TRILUTION Label Inside Rinse Drain XL 0mm rinse depth Right Well Outside Rinse XL Shallow 10 mm rinse depth Shallow Rinse Center Well Outside Deep Rinse Locations Left Well Outside Deep Rinse Left Wells Rinse XL Flow 45 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide suol e907 asuly Rinse Locations Rinse Locations Rinse Station 223 Tall Right Rack TRILUTION Label Inside Rinse Drain XL 0mm rinse depth Right Wells Outside Rinse XL Shallow 10 mm rinse depth Shallow Rinse Center Wells Outside Shallow Rinse Locations Center Well Outside Deep Rinse Left Wells Rinse XL Deep 85 mm wash cylinder G 20 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations GX 271 Liquid Handler and GX 271 ASPEC Bed Layout Tray Template GX 271 20 Series Racks GX 271 20 Series Racks with Direct Inject GX 271 200 Series Racks GX 271 200 Series Racks with Direct Inject GX Rinse Station Footprint GX Rinse Stations TRILUTION LH Software Version 2 0 User s Guide G 21 suol e907 asuly Rinse Locations Rinse Locations Drain Rinse Station for Inside Rinse of Probe Drain Rinse Station GX Extra Tall Rack Use Tube Top mm Rinse Station Tube Bottom mm Part Number Inside Rinse 1
349. ht mm The Z height to which the instrument s 122 Calculated based on Z Clamp probe moves before moving in the X or Y Height and Z Arm Type Range direction provided on screen Setup Syringe Pump ID A unique identifier to address syringeson A A the instrument Syringe Name A unique name to address syringes on A N A the instrument Size uL The capacity of each installed syringe For 1000 100 250 500 1000 5000 10000 a range of suggested flow rates for this 25000 syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid Reservoir N A that is flowing through the associated syringe It is important for sample tracking 215 Liquid Handler Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 9 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration 215 Liquid Handler Properties Continued Property Name Brief Description Default Value Range Contacts 24V Output Optionally type a description for the N A N A Output 1 contact This description will be used to Output 2 identify the contact in a Task Output 3 Output 4 Input A Optionally type a description for the N A N A Input B contact This description will be used to Input C identify the contact in a Task Input D General Instrument Name The name that will be used to identify this instrument in a Task Liquid Handler N A
350. ht from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate mM A U N Move Z top GAS AIR PURGE If SPE 215 TRUE 1 Move Z to top 2 Set Voltage On and Output Contact 1 to Close 3 Wait 0 02 min TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 4 Set Voltage Off and Output Contact 1 to Open 5 Move Z to top TRILUTION LH Software Version 2 0 User s Guide a ye1odeny Fractionate Tasks SPE D Fractionate If multiple analytes of interest will be collected use this task to aspirate the specified volume from the reservoir tray or transfer ports and then dispense it to the DEC with the mobile rack positioned over the specified collect zone For more information about the properties on each tab see Fractionate Properties on page D 53 Fractionate Advanced Rinsing on page D 57 Fractionate Instruments on page D 59 Fractionate Sequence of Steps on page D 60 D 52 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Fractionate Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample OuL Source Volume Reservoir The speed at which the Source Volume moves into and out of the probe For a 3 mL min Source Flow range of suggested flow rate values see Flow Rates on p
351. ht from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate mM A U N gt Move Z top GAS AIR PURGE If SPE 215 TRUE 1 Move Z to top 2 Set Voltage On and Output Contact 1 to Close 3 Wait 0 02 min TRILUTION LH Software Version 2 0 User s Guide D 115 ysem Tasks SPE D 4 Set Voltage Off and Output Contact 1 to Open 3 5 Move Z to top D 116 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Gilson supplies Tweaks tasks for use on the following instruments Task Liquid Handler Aspirate All except GX 271 GX 281 Liquid Handler with Solvent System and Quad Z 215 with Disposable Tips Aspirate with Tips Quad Z 215 with Disposable Tips Deliver All except GX 271 GX 281 Liquid Handler with Solvent System and Quad Z 215 with Disposable Tips Deliver with Tips Quad Z 215 with Disposable Tips Move To Move To Zone All All SS Aspirate GX 271 GX 281 Liquid Handler with Solvent System SS Deliver GX 271 GX 281 Liquid Handler with Solvent System TRILUTION LH Software Version 2 0 User s Guide E 1 Tasks Tweaks Tweaks Tweaks Tasks Gilson Task Name and Description Aspirate i This task aspirates liquid from the tray Aspirate with Tips This task aspirates liquid from the tray Deliver This task dispenses liquid through the probe
352. id Handler and ASPEC XL Custom Racks ASPEC XL4 Creating a Rack 223 Sample Changer Adding Elements GX 271 Liquid Handler and Creating a New Element GX 271 ASPEC Editing a Custom Element Viewing Elements Deleting Elements Deleting a Rack GX 274 Liquid Handler and GX 274 ASPEC GX 281 Liquid Handler Exporting Racks and Templates Importing Racks and Templates Closing Bed Layout and Utilities TRILUTION LH Software Version 2 0 User s Guide Electronic Record Management ERM GSIOC Configuration Editor Features GSIOC Configuration Editor ERM Administration Users and Groups TRILUTION LH Software Version 2 0 User s Guide K GSIOC Utility Starting the GSIOC Utility Reviewing the Port and Baud Information Listing GSIOC Instruments Basic Mode Basic Mode Buttons and Features Basic Mode Menus Advanced Mode Advanced Mode Buttons and Features Advanced Mode Menus Commands Immediate Command Buffered Command Insert an Immediate Command Insert a Buffered Command Send an Immediate Command Send a Buffered Command TRILUTION LH Software Version 2 0 User s Guide About the User s Guide This TRILUTION LH Software User s Guide is for the users of Gilson Liquid Handling instruments It provides instructions for TRILUTION LH The information contained in this document is intended for the laboratory operators or individuals doing research activities How to Use TRILUTION LH User s Guide The
353. ide D 69 peoy Load Tasks SPE D Load Properties Continued Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into and out of the probe For a 1 mL min Air Gap Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate DEC Zone The zone to which the Reservoir Volume is delivered DEC Well The well in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of probe For a range of 3 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 1 min Time SPE 215 Shake The SPE 215 Shake Off unseals the seal foot from the mobile rack Off Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Load Properties Table Page 3 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load Properties Continued Property Name Brief Description Default Value Solenoid Time the program waits after a s
354. ide Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Prime Pump Advanced Inside Rinse Table Page 2 of 3 B 128 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Prime Pump Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a
355. ies on each tab see Ay et Cherry Pick Properties on page A 26 Cherry Pick Advanced Rinsing on page A 27 Cherry Pick Instruments on page A 29 Cherry Pick Sequence of Steps on page A 30 TRILUTION LH Software Version 2 0 User s Guide A 25 Id Kay Cherry Pick Tasks Liquid Handling Cherry Pick Properties A Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source Volume is delivered Starting Well The well in the Result Z
356. ight Move probe to well in Inside Rinse Zone 1 2 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate 5 Dispense Inside Volume at Inside Rinse Flow Rate A 14 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OW A WU N gt TRILUTION LH Software Version 2 0 User s Guide ppv Aliquot Tasks Liquid Handling A Aliquot This task aspirates the liquid source from the reservoir tray or transfer ports and then dispenses that liquid equally to each of the wells of the result zone This task should be used in Batch mode only Properties Advanced ftiming fltastruments For more information about the properties on each tab see a Aliquot 60 Aliquot Properties on page A 17 Aliquot Advanced Rinsing on page A 19 Aliquot Instruments on page A 21 Aliquot Sequence of Steps on page A 22 A 16 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Aliquot Properties
357. ilder Configuration 5 Method Builder Bed Layout Configuration Tab Window Bed Layout Tab Window Methods Palette Methods Palette Configured Instruments Configured Instruments Instrument Types Zone Management Available Instruments Numbering Pattern Workspace Workspace Properties Creating a Bed Layout Creating a Configuration Instrument Properties Flow Rates Modifying a Bed Layout Deleting a Bed Layout Modifying a Configuration Clear Default TRILUTION LH Software Version 2 0 User s Guide 6 Method Builder Method 7 Method Builder Error Handling Method Tab Window Error Handling Tab Window Methods Palette Operators Palette Tasks Palette Workspace Setting Up Instrument Error Handling Parameter Descriptions Error Responses Modifying Instrument Error Handling Clear Default Error Name Creating a Method Variables TRILUTION LH Software Version 2 0 User s Guide 8 Application 9 Application Manual Control Application Window Manual Control Tab Window Title Bar Manual Control Properties Applications Palette by Instrument Sample List Run Name Bed Layout Bed Layout View Simulation Run Indicator Pressure Indicator Action Buttons Application Info Window Manual Instrument Control Setting Up a Sample List Default Columns Additional Columns Exporting a Sample List Importing a Sample List Run Results Window Result Exporting a Run Archiving a Run Restoring a Run TRILUTION LH
358. ile Rack Sequence of Steps on page D 100 OK Cancel Help Move Mobile Rack Properties Property Name Brief Description Default Value Collect Drain Select where to move mobile rack Collect Drain or Initialize Mobile Rack Collect Initialize Mobile Rack DEC Zone Name The zone name Move Mobile Rack Properties D 99 TRILUTION LH Software Version 2 0 User s Guide yey a IqoW AOW Move Mobile Rack Tasks SPE D Move Mobile Rack Instruments Property Name Default Value Liquid Handler Move Mobile Rack Instruments Move Mobile Rack Sequence of Steps 1 Move Z to top 2 Move mobile rack Collect Zone over drain or the collect 3 Move Z to top D 100 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Wash This task washes the DEC by aspirating a specified volume of liquid source from the reservoir tray or transfer ports and then dispensing the volume to the wells of the DEC zone with the mobile rack positioned over the drain For more information about the properties on each tab see Wash Properties on page D 102 Wash Advanced Rinsing on page D 106 Wash Instruments on page D 108 Wash Sequence of Steps on page D 109 TRILUTION LH Software Version 2 0 User s Guide D 101 ysem Wash Tasks SPE D Wash Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port
359. ilutor select the liquid handler Injector The injector or injection module that the task will affect Inject Instruments A 77 TRILUTION LH Software Version 2 0 User s Guide Inject Tasks Liquid Handling A Inject Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 78 2 Dispense on page A 78 3 Inside Rinse on page A 79 4 Outside Rinse on page A 79 ASPIRATE Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Set injection valve position to Load N WO UW A UN gt DISPENSE Move probe to Injection Well in Injection Zone Lower probe into well to Inject Height Dispense Source Volume at Injection Flow Rate Wait Equilibration Time Set injection valve position to Inject Move Z to Z Safe Height Nun A U N gt A 78 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling 7 Move probe to well in Inside Rinse Zone 8 Lower probe into well to Inside Rinse Height 9 Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate 5 Dispe
360. ilutor and sends the pistons to the upper position with the valves in the probe position LOWER SYRINGE Descends the syringes piston operating rods 25 of a full stroke as the syringe pump aspirates from the reservoir The valves will switch to the outlet position RAISE SYRINGE Homes the dilutor and sends the pistons to the upper position with the valves in the probe position SPE 215 Manual Control Table Page 3 of 3 Used when replacing a syringe 9 72 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control VALVEMATE The VALVEMATE Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons Property VALVEMATE Manual Control Nae Brief Description Default Value Use Valve The number of ports in the VALVEMATE valve 2 Position Rotates the valve to the selected position 1 Click SWITCH Button HOME VALVE Rotates the valve to home position 1 VALVEMATE Manual Control TRILUTION LH Software Version 2 0 User s Guide MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control 9 VALVEMATE II The VALVEMATE II Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons VALVEMATE II Manual Control
361. ime Volume at Result Flow Rate Move Z to top INSIDE RINSE OPTIONAL 1 2 3 4 5 6 Move Z to top Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Flow Rate Dispense Inside Volume at Inside Flow Rate Repeat steps 4 5 until Inside Volume delivered TRILUTION LH Software Version 2 0 User s Guide C 65 sdi yyim dung dawg Prime Pump with Tips Tasks Disposable Tips C OUTSIDE RINSE OPTIONAL Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate OAU A U N gt Repeat steps 4 5 until Outside Volume delivered RINSE TOUCH OFF Side 1 Move Z to top 2 Move probe to well in Inside Rinse Zone 3 Move probe to Side Rinse Z Offset 4 Move probe 1 5 mm in the Y direction forward 5 Move probe to Side Rinse Y Offset 6 Move probe to the center of the well 7 Move Z to top Surface Move Z to top Move probe to well in Outside Rinse Zone Move probe to Surface Rinse Z Offset AA U N gt Move Z to top C 66 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips None 1 Move Zto top TRILUTION LH Software Version 2 0 User s Guide C 67 sdi yyim dung dawg Prime Rinse Station Tasks Disposable Tips C Prime Rinse Station This task
362. in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List SPE 215 The SPE 215 Shake Off unseals the seal foot from the mobile rack Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Solenoid Time the program waits after a solenoid air push before moving out of the well 0 01 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 20 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 10 mL min Aspirate Flow Rate suggested flow rate values see Flow Rates on page 4 93 Dry Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Dry Properties Continued Property Name Brief Description Default Value Syringe The speed at which the Air Push Volume moves out of probe For a range of 10 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 01 min Equilibration Time Valve Transfer tubing volume
363. ing Pattern Numbering Pattern allows you to select how blocks of wells are numbered Ij 0 0 0 The Numbering Pattern To select a pattern for numbering the wells do the following 1 Click on the desired pattern 2 Click on the starting corner desired for the pattern the well is highlighted red Workspace Forms the area to create or view a Bed Layout TRILUTION LH Software Version 2 0 User s Guide Method Builder Bed Layout Creating a Bed Layout 1 Create a Configuration Refer to Creating a Configuration on page 4 5 if necessary 2 Doone of the following To use the displayed template continue to Step 3 Click TEMPLATES Select a Bed Layout template and click OK The new template displays Click BROWSE Select an existing or imported Bed Layout and click OK The selected template displays 3 To adda Rack do the following a Double click a footprint b From the Select Rack window select a Rack name c Click OK The window displays rack on the template 4 Select a zone Refer to Zone Management on page 5 3 if necessary 5 Select anumbering pattern Refer to Numbering Pattern on page 5 6 if necessary TRILUTION LH Software Version 2 0 User s Guide 5 7 3noKe7 pag e GuljeasD Creating a Bed Layout Method Builder Bed Layout 5 8 6 To add wells to a zone do any of the following a Hold SHIFT and then click on individual wells to number them in the order selected b Lasso b
364. inge Prime Flow Rate The speed at which the reservoir fluid moves into and out of the syringes 10 mL min Use Click PRIME to start or STOP PRIME to end Buttons HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 406 Pump Dual Manual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 9 25 MOpUIM qe O4 U0D jenuew Manual Control Tab Window Application Manual Control 9 Property Name LOWER SYRINGE 406 Pump Dual Manual Control Continued Brief Description Default Value Use Used when replacing a syringe Descends the active syringe s piston operating rod 25 of a full stroke RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 406 Pump Dual Manual Control Table Page 2 of 2 9 26 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 406 Pump Single The 406 Pump Single Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 406 Pump Single Manual Control Default Value Use 10 mL min Click PRIME to start or STOP PRIME to end Property Name Brief Description Prime Flow Rate The speed at which the r
365. inse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Dispense Random to Random Advanced Rinsing Table Page 3 of 3 Dispense Random to Random Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Dispense Random to Random Instruments TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Dispense Random to Random Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 71 2 Dispense on page A 71 3 Inside Rinse on page A 72 4 Outside Rinse on page A 72 ASPIRATE Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height On A U N gt DISPENSE Move probe to Result
366. inse Z Option SS Aliquot Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the outside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered for the Outside Rinse Z Offset the probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Aliquot Outside Rinse Table Page 2 of 4 0 mm T
367. ion Click MOVE X Y Y Enter a number to move to a position forward of 0 0 mm the home position Z Enter a number to move to an absolute Z value 0 0 mm Click MOVE Z ASPEC XL Manual Control Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 33 MOpUIM qe J01 Uu0 enuey Manual Control Tab Window Application Manual Control 9 ASPEC XL Manual Control Continued ore Brief Description Default Value Use Name Relay Outputs 1 A filled box indicates that the circuit is closed on Toggle the contacts open closed 2 An empty box indicates that the circuit is open off on by selecting and clearing 3 off the boxes Output Contacts 4 A filled box indicates that the circuit is closed on Toggle the contacts open closed 5 An empty box indicates that the circuit is open off on by selecting and clearing 6 off the boxes 7 8 Input Contacts 1 A filled box indicates that the circuit is closed An Monitor status 2 empty box indicates that the circuit is open 3 Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration ASPEC XL Manual Control Table Page 2 of 3 9 34 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 Property Name UNLOCK ARM ASPEC XL Manual Control Continued Brief Description Default Va
368. ion The syringes that will be used Default Value Left Syringe Right Syringe Prime Flow Rate The speed at which the reservoir fluid moves into and out of the syringes 10 mL min Use Click PRIME to start or STOP PRIME to end Buttons HOME SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 402 Pump Dual Manual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide MOpUIM qe O4 U0D jenuew Manual Control Tab Window Application Manual Control 9 402 Pump Dual Manual Control Continued Brief Description Default Value Use Property Name LOWER SYRINGE Descends the active syringe s piston operating rod 25 of a full stroke as the syringe pump aspirates from the reservoir The valve will switch to the outlet position Used when replacing a syringe RAISE SYRINGE Homes the dilutor and sends the piston to the upper position with the valve in the probe position 402 Pump Dual Manual Control Table Page 2 of 2 9 20 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 402 Pump Dual with Tee 402 Pump Dual with Tee Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 402 Pump Dual with Tee Manual Control Property
369. ion of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Dispense Random to Random Advanced Outside Rinse Table Page 3 of 4 B 96 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dispense Random to Random Advanced Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Dispense Random to Random Advanced Outside Rinse Table Page
370. ion valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Cherry Pick Outside Rinse Table Page 4 of 4 SS Cherry Pick Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Cherry Pick Outside Rinse B 41 TRILUTION LH Software Version 2 0 User s Guide Id D SS SS Cherry Pick Tasks SS Liquid Handling B SS Cherry Pick Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 42 2 Dispense on page B 42 3 Inside Rinse on page B 43 4 Outside Rinse on page B 43 ASPIRATE Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move to Source Well in Source Zone Lower probe into well to Source Height Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Nun A U N gt DISPENSE 1 Move probe to starting well in Result Zone Note Well is automatically incremented
371. iption Default Value Transfer Port The zone from which the Result Volume is drawn Source Zone Transfer Port Select a Transfer Port to activate for the task A Transfer Port Transfer Port An additional volume to the Result Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Result Volume Transfer Port The quantity of the air gap aspirated before the Result Volume 20 uL Source Air Gap Transfer Port The speed at which the Result Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Result Volume is delivered Result Well The well in the Result Zone to which the Result Volume is delivered When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Result Volume The quantity of liquid delivered to the Result Well in the Result Zone O uL Aliquot Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Aliquot Properties Continued Property Name Brief Description Default Value Resul
372. iption Default Value Tray The quantity of the air gap aspirated before the Diluent Volume 20 uL Diluent Air Gap Tray The speed at which the Diluent Volume moves into the probe For a range of 5 mL min Diluent Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Diluent Air Gap Volume moves into the probe For a range 0 3 mL min Air Gap Flow of suggested flow rate values see Flow Rates on page 4 93 Rate Reservoir The quantity of liquid used to dilute the Source Volume 0 Diluent Volume Reservoir The speed at which the Diluent Volume moves into of the probe For a range of 10 mL min Diluent Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Diluent Volume is drawn Diluent Zone Transfer Port Select a Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of liquid used to dilute the Source Volume OuL Diluent Volume Dilute Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide A 47 ain id Dilute Tasks Liquid Handling Dilute Properties Continued A Property Name Brief Description Default Value Transfer Port The quantity of the air gap aspirated before the Diluent Volume 20 uL Diluent Air Gap Transfer Port The speed at which the Diluent Volume moves into the probe For a range of 5 mL min Diluent Flow suggested flow rate values see Flow Rates on page
373. ircuit is open D Probe Spacing Enter a value between 9 0 and 18 0 Buttons Quad Z 215 Man ual Control Table Page 2 of 3 9 68 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 Quad Z 215 Manual Control Continued oar Brief Description Default Value Use Name HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position Quad Z 215 Manual Control Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 69 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 9 SPE 215 The SPE 215 Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons SPE 215 Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up whena positive number 2mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom SPE 215 Manual Control Table Page 1 of 3 9 70 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control SPE 215 Manu
374. ith Tips Properties on page C 78 Transfer with Tips Tips on page C 79 Transfer with Tips Advanced Rinsing on page C 82 Transfer with Tips Instruments on page C 83 Transfer with Tips Sequence of Steps on page C 84 TRILUTION LH Software Version 2 0 User s Guide C 77 sdi YUM JajsuesL Transfer with Tips Tasks Disposable Tips Transfer with Tips Properties Property Name Brief Description Default Value Source Select Reservoir or Tray Tray Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn 1 Source Well Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Reservoir The quantity of a sample OuL Source Volume Transfer with Tips P
375. ition Reservoir The quantity of a sample O uL Source Volume Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn 1 Source Well Tray Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray The quantity of a sample O uL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap SS Dispense Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide B 77 su ds d SS SS Dispense Tasks SS Liquid Handling SS Dispense Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the
376. ive number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Transfer Advanced Rinsing Table Page 1 of 2 2mm TRILUTION LH Software Version 2 0 User s Guide A 111 JaJSULAL Transfer Tasks Liquid Handling Property Name Inside Rinse Zone Transfer Advanced Rinsing Continued Brief Description The zone to which the Inside Volume is delivered A Default Value Inside Rinse Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and intothe 10 mL min Rate outside rinse position in the rinse sta
377. l move when performing a side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Prime Pump with Tips Advanced Rinsing Table Page 3 of 3 Prime Pump with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Prime Pump with Tips TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Prime Pump with Tips Sequence of Steps The following is an overview for the sequence of steps 1 2 3 4 Aspirate on page C 65 Inside Rinse Optional on page C 65 Outside Rinse Optional on page C 66 Rinse Touch Off on page C 66 ASPIRATE On A WN Move Z to top Aspirate the Prime Volume at Source Flow Rate Move probe to Result Well in Result Zone Lower probe into well to Result Height Dispense Pr
378. l to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OO UW A U N gt TRILUTION LH Software Version 2 0 User s Guide A 87 XIW Prime 402 Dual with Tee Tasks Liquid Handling A Prime 402 Dual with Tee This task primes the 402 Pump Dual with Tee in the specified zone It aspirates the full syringe capacity for each syringe and dispenses it to the wells in the result zone the specified number of cycles asi Panacan liiam For more information about the properties on each tab see Prime 40 Dual with T o Prime 402 Dual with Tee Properties on page A 89 Prime 402 Dual with Tee Advanced Rinsing on page A 89 Prime 402 Dual with Tee Instruments on page A 91 Prime 402 Dual with Tee Sequence of Steps on page A 91 A 88 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Prime 402 Dual with Tee Properties A Property Name Brief Description Default Value Source Source for the Prime 402 Dual Pump with Tee is Reservoir Reservoir Result Zone The zone to which the Syringe Volume is delivered Inside Rinse Result Well The well in the Result Zone to which the Syringe Volume is delivered When using 1 a multiple probe instrument type x where xis a value defined in the Sample
379. l using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Dispense Advanced Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide B 83 su ds d SS SS Dispense Tasks SS Liquid Handling SS Dispense Advanced Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rin
380. lder Configuration 4 406 Pump Dual D w B iw kef sl HH 1 ID Syringe Name Size uL Reservoir Name gt A A 1000 Reservoir B B 1000 Reservoir The 406 Pump Dual Properties Window Properties 406 Pump Dual Properties Default Property Name Brief Description Value Range Setup Syringes to Use The syringes that will be used when this A B A B instrument is selected as the Pump in a Task ID A unique identifier to address syringes on the A B A B instrument 406 Pump Dual Properties Table Page 1 of 2 4 28 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 406 Pump Dual Properties Continued eno Default Property Name Brief Description Value Range Syringe Name A unique name to address syringes on the A B N A instrument Size uL The capacity of the installed syringe Fora rangeof 10000 100 250 500 1000 5000 suggested flow rates for this syringe size see Flow 10000 25000 Rates on page 4 93 Reservoir Name A unique name used to identify the liquid thatis Reservoi N A flowing through the associated syringe It is r important for sample tracking General Instrument Name The name that will be used to identify this Pump N A instrument in a Task Pump Serial Number The serial number of the pump The serial number N A N A is located on the rear panel and near the bottom of the instrument 406 Pump Dual Properties Table Pa
381. le List and the mode will be set to Sequential For more information on sequential processing see Sequential on page 8 17 If SEQUENTIAL MULTIPLE each well selected will be added as its own row in the Sample List and the mode will be set to Sequential For more information on sequential processing see Sequential on page 8 17 3 If BATCH all wells selected will be added as one row in the Sample List and the mode will be set to Batch For more information on batch processing see Batch on page 8 17 8 10 TRILUTION LH Software Version 2 0 User s Guide Application 8 Simulation A graphical representation of a Run is referred to as a Simulation To simulate a Run 1 Create or Open an Application Click to select the Simulation tab In the Set Simulation Speed field select a speed from the drop down list Use the Scale Factor options to zoom if desired 5 Click SIMULATE Run Indicator The Run Indicator is a graphical representation of the progress of the run Pressure Indicator The Pressure Indicator is used to monitor the pressure of the 406 Pump Single and or 406 Pump Dual during the run TRILUTION LH Software Version 2 0 User s Guide 8 11 MOpul UO ed1 ddy Application Window Application 8 12 Action Buttons Application The following action buttons are located in the lower left corner of the Application window Action Button Description RUN PAUSE RESUME SIMULATE
382. le click on the name of the Task e Right click on a task and click OPEN from the submenu e Click OPEN The Open Task window displays a Clear the Show Latest Versions check box to view all versions of all Tasks that have been created on the computer and have not been deleted b Select the Task c Click OK The Task displays in the workspace Multiple Tasks can be open in the same builder Individual tabs display at the top of the window for each open Task Click on the individual tab to view the Task within the workspace TRILUTION LH Software Version 2 0 User s Guide yseL e DuimaN Modifying a Task Task Builder 2 Modifying a Task You can modify a Task by doing the following e Adding Tasks Commands Variables Operators and Expressions to the workspace e Deleting Tasks Commands Variables Operators and Expressions from the workspace e Modifying the properties of the Tasks Commands Variables Operators and Expressions in the workspace e Changing the description or custom icon When you right click on a Task Command Variable Operator or Expression in the workspace a menu displays with the following options CUT COPY PASTE DELETE LINE BREAK VIEW Or PROPERTIES Right click on Tasks also provides an OPEN TASK option To paste right click anywhere in the workspace and select PASTE from the menu Modifying Tasks 1 To open a Task see Viewing a Task on page 2 11 2 To make chang
383. le the contacts open closed 2 closed on An empty box indicates that off on by selecting and clearing 3 the circuit is open off the boxes Output Contacts 4 A filled box indicates that the circuit is Toggle the contacts open closed 5 closed on An empty box indicates that off on by selecting and clearing 6 the circuit is open off the boxes 7 8 Input Contacts 1 A filled box indicates that the circuit is Monitor status 2 closed An empty box indicates that the 3 circuit is open ASPEC XL4 Manual Control Table Page 2 of 4 TRILUTION LH Software Version 2 0 User s Guide 9 37 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control ASPEC XL4 Manual Control Continued Property Name Brief Description Default Value Use Pump Active Syringes The syringes that will be used A B C D Click PRIME to start or STOP PRIME to Prime Flow Rate The speed at which the reservoir fluid 10 mL min and moves into and out of the syringes Solvent Station Active Solvent Port The solvent port that will be used A Click PRIME to start or STOP PRIME to Prime Flow Rate The speed at which the reservoir fluid 10 mL min moves into and out of the solvent port end Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM
384. lect box to mix with liquid Number of Number of times the task should repeat the commands related to mixing 1 Mixes Mix Air Gap The quantity of the air gap aspirated before the Mixing Volume 20 uL Mixing Volume The quantity of air or liquid used as part of a mixing process O uL Mixing Flow The speed at which the mixing volume moves into the probe For a range of 10 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction SS Derivatize Properties Table Page 3 of 3 B 48 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Liquid Level Following SS Derivatize Advanced Inside Rinse Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height Solution Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height Solution Height and Result Height to the height at which the liquid was dete
385. lick MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom GX 274 ASPEC without Pump Manual Control Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 53 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control GX 274 ASPEC without Pump Manual Control Continued ore Brief Description Default Value Use Name X Enter anumber to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter anumber to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0mm l Click MOVE Z value Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off on 2 closed on An empty box indicates that by selecting and clearing the boxes the circuit is open off 24V Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off on 2 closed on An empty box indicates that by selecting and clearing the boxes the circuit is open off Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the circuit is open GX 274 ASPEC without Pump Manual Control Table Page 2 of 3 9 54 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control GX 274 ASPEC withou
386. liquid through the probe E 10 For more information about the properties on each tab see Deliver Properties on page E 11 Deliver Instruments on page E 11 Deliver Sequence of Steps on page E 12 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Deliver Properties Property Name Brief Description Default Value Result Volume The quantity of liquid delivered O uL Result Flow Rate The speed at which the Result Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after the dispense 0 min Time Deliver Properties Deliver Instruments Default Value Property Name Liquid Handler Brief Description The liquid handler that the Task will affect Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Deliver Instruments TRILUTION LH Software Version 2 0 User s Guide E 11 JIAIEQG Deliver Tasks Tweaks Deliver Sequence of Steps 1 Dispense Result Volume at Result Flow Rate 2 Wait Equilibration Time E 12 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks E Deliver with Tips This task dispenses liquid through the probe Properties instruments For more information about the properties on each tab see Deliver with Tips p Deliver with Tips Prope
387. liquot Advanced Rinsing Continued Property Name Brief Description Default Value Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Aliquot Advanced Rinsing Table Page 3 of 3 Aliquot Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Aliquot Instruments A 21 TRILUTION LH Software Version 2 0 User s Guide yonbily Aliquot Tasks Liquid Handling Aliquot Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 22 2 Dispense on page A 23 3 Inside Rinse on page A 24 4 Outside Rinse on page A 24 ASPIRATE Reservoir 1 Move Z to Z Safe Height 2 Aspirate aliquot volume Resul
388. locks of wells to number them according to the numbering pattern selected c When using multiple layer racks hide one layer to assign zone s to another To do this select highlight the element then right click and select HIDE from the menu Select SHOW ALL to unhide the elements If the liquid handler is a Quad Z 215 Liquid Handler with Disposable Tips be sure to use the tip zones TRILUTION LH created for tips 7 Inthe workspace do the following a To zoom the Bed Layout toggle the Auto Scale selection off b To zoom in on the Bed Layout click once or repeatedly maximum scale factor 100 increments by 5 TRILUTION LH will zoom in to the center of the cross hairs Move the cross hairs by scrolling the window c To zoom out on the Bed Layout click once or repeatedly d To zoom to a specific scale factor enter the number in the Scale Factor field TRILUTION LH Software Version 2 0 User s Guide Method Builder Bed Layout Modifying a Bed Layout You can modify a Bed Layout by doing the following Add new racks wells or zones to the template Delete racks from the workspace Delete zones Change the numbering pattern Replace racks in the workspace Add or clear wells from zones When you right click on a rack or well in the workspace a menu is provided with options to delete the rack or clear the well from the active zone or from all zones To modify a Bed Layout 1 Do any of the follo
389. lue Use Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position ASPEC XL Manual Control Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 35 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control ASPEC XL4 The ASPEC XL4 Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons ASPEC XL4 Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number 2mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom ASPEC XL4 Manual Control Table Page 1 of 4 9 36 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control ASPEC XL4 Manual Control Continued Property Name Brief Description Default Value Use X Enter a number to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0mm l Click MOVE Z value Relay Outputs 1 A filled box indicates that the circuit is Togg
390. lue defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 3 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 1 min Time SPE 215 Shake The SPE 215 Shake Off unseals the seal foot from the mobile rack Off Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Wash Properties Table Page 3 of 5 D 104 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Wash Properties Continued Property Name Brief Description Default Value Solenoid Time the program waits after a solenoid air push before moving out of the well 0 1 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 20 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 6 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of the probe Fora range of 6 mL min Dispense Flow suggested flow rate
391. mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Result Zone The zone to which the Source Volume are delivered Inside Rinse Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Prime Pump with Tips Properties Prime Pump with Tips Advanced Rinsing Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Prime Pump with Tips Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Property Name Outside Rinse Zone Prime Pump with Tips Advanced Rinsing Continued Brief Description The zone to which the Outside Volume is delivered Default Value Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No rinse is performed 0 uL when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min
392. mL min Rate suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after the aspirate 0 min Time Aspirate Properties Table Page 2 of 2 Aspirate Instruments Default Value Property Name Liquid Handler Brief Description The liquid handler that the Task will affect Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Aspirate Instruments TRILUTION LH Software Version 2 0 User s Guide E 5 a esdsy Aspirate Tasks Tweaks Aspirate Sequence of Steps 1 Aspirate Source Volume at Source Flow Rate 2 Wait Equilibration Time E 6 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks E Aspirate with Tips This task aspirates liquid from the tray Properties J Tips Enstruments For more information about the properties on each tab see Aspirate with Tip Aspirate with Tips Properties on page E 7 Aspirate with Tips Tips on page E 8 source volume tut fo Aspirate with Tips Instruments on page E 8 Source Flow Rate mi fminy 5 Equilitration Time min f Aspirate with Tips Sequence of Steps on page E 9 Property Name Source Volume Aspirate with Tips Properties Table Page 1 of 2 sdi y m pe dsy TRILUTION LH Software Version 2 0 User s Guide E 7 Aspirate with Tips Tasks Tweaks Aspirate with Tips Properti
393. mber is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and intothe 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Dilute Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Dilute Advanced Rinsing Continued A Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distan
394. mber is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Dispense Random to Random Advanced Inside Rinse Table Page 2 of 3 B 92 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Dispense Random to Random Advanced Inside Rinse Continued Brief Description One of six defined reference points sel
395. mber to move to a position to 0 0 mm the right of the home position Click MOVE X Y Y Enter a number to move to a position 0 0 mm forward of the home position Z Enter a number to move to an absolute Z 0 0 mm Click MOVE Z value Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the the circuit is open off boxes 24V Output Contacts 1 A filled box indicates that the circuit is Toggle the contacts open closed off 2 closed on An empty box indicates that on by selecting and clearing the the circuit is open off boxes Input Contacts A A filled box indicates that the circuit is Monitor status B closed An empty box indicates that the circuit is open GX 271 ASPEC without Pump Manual Control Table Page 2 of 3 9 44 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control GX 271 ASPEC without Pump Manual Control Continued Property Name Brief Description Default Value Use Solenoids 1 A filled box indicates that the solenoid is 2 on An empty box indicates that the solenoid is off Injection Valve Indicate whether a GX Direct Inject Valve is installed Valve Position Rotates the valve to the selected position Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in t
396. me 50 uL Source Air Gap Load Properties Table Page 1 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into and out of the 3 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into and out of the probe For a 1 mL min Air Gap Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select the Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into and out of the 3 mL min Source Flow probe For a range of suggested flow rate values see Flow Rates on page 4 93 Rate Load Properties Table Page 2 of 5 TRILUTION LH Software Version 2 0 User s Gu
397. ment detects the liquid surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Source Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Dispense Advanced Rinsing Table Page 1 of 2 2mm TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Inside Rinse Zone Dispense Advanced Rinsing Continued Brief Description The zone to which the Inside Volume is delivered A Default Value Inside Rinse Outside Rinse Zone
398. ms to be exported Aspirate with Tips Cherry Pick Cherry Pick with Tips Condition Deliver Deliver with Tips Derivatize Dilute Dispense RDienanen Dandam ta Dandam ARR RRP RP RP RP RRP Re Select a path wie Cancel The Select items to Export window TRILUTION LH Software Version 2 0 User s Guide 2 15 sse Bulz1odxy Exporting Tasks Task Builder 2 16 In the Select items to Export window complete the following a In the Select the items to be exported field select the Task s To select multiple Tasks in a sequential order hold SHIFT any arrow key simultaneously To select Tasks in a random order hold CTRL and select multiple Tasks with the mouse To select all Tasks click To clear all selected Tasks click To invert the selection of Tasks click a b In the Select a path field complete the following 1 Click The Browse For Folder window displays 2 On the Browse For Folder window select a folder and click OK Click OK On completion of the Export operation the Task is saved in the specified folder with a LHTE extension The TRILUTION information dialog box displays 4 gt If a Task s with the same name is found in the Export path an option is provided to rename or overwrite the Task or to skip the Export operation for that Task a On the TRILUTION information dialog box do the following e To view the Export operation log information click DETAILS
399. n mM A U N gt Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume B 74 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling 6 Switch Peristaltic Rinse Pump off 7 Move Z to Z Safe Height 8 Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station TRILUTION LH Software Version 2 0 User s Guide 23Nn I0 SS SS Dispense Tasks SS Liquid Handling SS Dispense This task dispenses the specified volume of liquid source from the reservoir or tray to the wells of the result zone Properties TAS PERC RTE B 76 For more information about the properties on each tab see SS Dispense Properties on page B 77 SS Dispense Advanced Inside Rinse on page B 79 SS Dispense Advanced Outside Rinse on page B 82 SS Dispense Instruments on page B 85 SS Dispense Sequence of Steps on page B 86 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dispense Properties Property Name Brief Description Default Value Source Select Reservoir or Tray Reservoir Reservoir Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Pos
400. n Result Zone Lower probe to Result Height from Tube Bottom Aspirate syringe volume from Reservoir with the left syringe Dispense syringe volume with the left syringe Repeat steps 4 5 for the Number of Cycles syringe from Reservoir TRILUTION LH Software Version 2 0 User s Guide Aspirate the volume needed to prime the right syringe right syringe volume x Number of Cycles using the left L YUM jena ZOp awd Prime 402 Dual with Tee Tasks Liquid Handling A 8 Dispense the right syringe volume using the left syringe while simultaneously aspirating using the right syringe 9 Dispense the right syringe column using the right syringe 10 Repeat steps 8 9 for the Number of Cycles Repeat step 7 if necessary INSIDE RINSE Move Z to Z Safe Height Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume delivered Move Z to Z Safe Height N OW A U N gt OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OAU A U N gt A 92 TRILUTION LH Softwar
401. n XL Short Left Rack TRILUTION Label Inside Rinse Location Left Well Inside Rinse Left Well Drain XL 0mm rinse depth Outside Shallow Rinse Location Center Well Outside Shallow Rinse Center Well Rinse XL Shallow 10 mm rinse depth Outside Deep Rinse Location Right Well Outside Deep Rinse Right Well Rinse XL Flow 45 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide suol e907 asulYy Rinse Locations Rinse Locations Rinse Station XL Tall Left Rack TRILUTION Label Inside Rinse Left Well Drain XL 0mm rinse depth Outside Shallow Rinse Location Center Well Outside Shallow Rinse Center Well Rinse XL Shallow 10mm rinse depth n Outside Deep Rinse Location Right Well Outside Deep Rinse Right Well Rinse XL Deep 85 mm wash cylinder G 8 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations 222 XL Liquid Handler and ASPEC XL Bed Layout Trays Templates ASPEC XL Large XL Small Bar Large XL Code 30 Series Large XL Code 20 and 60 Series Heightener Kit Large XL Code 20 and 60 Series Port Bar Large XL Footprint Port Bar Large XL 2 Injection Ports Rack Port Bar Large XL 2 Injection Ports Only Rack Port Large All Transfer Rack Port Bar Large XL Antler Code 0 Series Rack Rinse Station XL Left Footprint a O
402. n the X direction SS Dispense Random to Random Properties Table Page 2 of 2 SS Dispense Random to Random Advanced Inside Rinse Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive SS Dispense Random to Random Advanced Inside Rinse Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide B 91 wopuey 0 wopuey su ds q SS SS Dispense Random to Random Tasks SS Liquid Handling SS Dispense Random to Random Advanced Inside Rinse Continued Property Name Brief Description Default Value Source Height The distance up when a positive nu
403. nce of steps 1 Load Tips Optional on page C 38 2 Aspirate on page C 38 3 Dispense on page C 40 4 Eject Tips Optional on page C 42 5 Inside Rinse Optional on page C 43 6 Outside Rinse Optional on page C 43 7 Rinse Touch Off on page C 43 LOAD TIPS OPTIONAL 1 Move Z to top 2 If Load Tips before Task TRUE load tips ASPIRATE Reservoir 1 Move Z to top 2 Move probe to Result Well in Result Zone 3 Lower probe into well to Result Height 4 Aspirate Source Volume from Reservoir at Source Flow Rate C 38 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Tray if Extra Volume 0 1 2 3 4 Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Prewet Tips only 1st Iteration a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if selected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected b If Prewet Tip FALSE 1 Skip to step 5 Aspirate Source Volume at Source Flow Rate using Liquid Level Following if selected Move Z to top Tray if Extra Volume gt 0 U A U N Move Z to top Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height Prewet Tips only 1st Iteration a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Lev
404. ne Properties Advanced ttinsing Pimstrwwents FOr more information about the properties on each tab see 6 Dispense os Dispense Properties on page A 57 Dispense Advanced Rinsing on page A 60 Dispense Instruments on page A 62 Dispense Sequence of Steps on page A 62 A 56 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Dispense Properties Property Name Brief Description Default Value Source Select Reservoir Tray or Transfer Port Reservoir Reservoir The quantity of a sample O uL Source Volume Reservoir The speed at which the Source Volume moves into the probe For a range of 10 mL min Source Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where xis a value defined in the Sample List Tray The quantity of a sample O uL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Dispense Properties Table Page 1 of 3 TRILUTION LH Software Versi
405. ne of the command you want to send TRILUTION LH Software Version 2 0 User s Guide K 25 AINN DOISD
406. nfiguration Al A Configuration consists of a group of uniquely named instruments The Configuration is part of a Method The drag and drop feature enables easy creation of Configurations by dragging an instrument and dropping it into the Workspace TRILUTION LH Software Version 2 0 User s Guide 4 1 Configuration Tab Window Method Builder Configuration 4 2 Configuration Tab Window The Configuration window is a graphical interface used to create a Configuration feeatable gad Hissdir ASE ASEC RA ASET GLUI ASPEC waa Pary GNT Lee Maar The Method Builder Configuration Tab Window TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration It has the following sections Section Described on Methods Palette page 4 3 Configured Instruments page 4 3 Instrument Types page 4 4 Available Instruments page 4 4 Workspace page 4 4 Properties page 4 4 Methods Palette This palette lists the Methods created Configured Instruments This palette is used to provide information and also for navigation Information provided is dependent on the instrument type Liquid Handlers Lists Bed Layout Can navigate to the Bed Layout tab by clicking the Bed Layout name Pumps Used only for navigation Click on the instrument name to view the property page for that instrument Accessories Used only for navigation Click on the instrument name to view the property p
407. ng to the Result Zone 2mm Inside Rinse Zone The zone to which the Inside Volume is delivered Inside Rinse Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Add Advanced Rinsing Table Page 2 of 3 A 10 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Add Advanced Rinsing Continued A Property Name Brief Description Default Value Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom fo
408. nique GSIOC ID for each TRILUTION LH Software Version 2 0 User s Guide 4 91 uoljeinbiyuo gt e Nurze Creating a Configuration Method Builder Configuration 4 4 Drag and drop a Virtual Pumping System into the workspace a Click on a Syringe Name and then click the up or down arrow to associate the syringe with a probe b In the VALVEMATE field click the arrow and then select the VALVEMATE from the drop down list c Inthe Position field click the arrow and then select the VALVEMATE valve position from the drop down list that corresponds to the syringe d Inthe Pump Name field type a name for the VPS This name will be used to identify the VPS in a Task 5 Repeat step 4 for each additional syringe 4 92 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration Flow Rates Syringe Pumps The following table provides suggested flow rate values by instrument and syringe size Instrument 402 eee GX 271 274 215 Se ae ASPEC XL4 ASPEC Multiple Probe Liquid Handler ar 404 Syringe Pump 406 Single 215 444 QuadDilutor oual Min Max Min Max Min Max Min Max Syringe Size pL i mL min mL min mL min mL min SPE 215 Min Max mL min TRILUTION LH Software Version 2 0 User s Guide 4 93 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration 4 Minipuls 3 For Minipuls 3
409. nless Liquid Level Detection or Liquid Level Following is selected when aspirating the Mix Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Mix Zone Mix Advanced Rinsing Table Page 1 of 2 2mm TRILUTION LH Software Version 2 0 User s Guide XIW Mix Tasks Liquid Handling Property Name Inside Rinse Zone Mix Advanced Rinsing Continued Brief Description The zone to which the Inside Volume is delivered A Default Value Inside Rinse Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distan
410. no stop pin was used select 175 mm Probe GX 274 Liquid Handler without Pump Properties Table Page 1 of 3 175 mm Probe 4 62 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 4 GX 274 Liquid Handler without Pump Properties Continued uoleinbyuos e Gules Property Name Brief Description Default Value Range Z Clamp Height mm This number corresponds to where the mounting 175 Calculated based clamp is on the vertical ruler on the installed on Z Clamp Height Z arm and Probe Type Range provided on screen Z Safe Height mm The Z height at which the instrument s probe 172 Calculated based moves to before moving in the X or Y direction on Z Clamp Height and Probe Type Range provided on screen Contacts Output 1 Optionally type a description for the contact This N A N A Output 2 description will be used to identify the contactina 24V Output 1 Task eo ues Additionally indicate whether the contact should open close or remain in the same state on error The default is No Change for Outputs 1 and 2 and Open for 24V Outputs 1 and 2 Input A Optionally type a description for the contact This N A N A Input B description will be used to identify the contactina Task GX 274 Liquid Handler without Pump Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 63 Creating a Configuration Method Builder Configuration GX 2
411. nse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Cherry Pick Outside Rinse Table Page 3 of 4 B 40 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Cherry Pick Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent select
412. nse Inside Volume at Inside Rinse Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OM A WU N gt TRILUTION LH Software Version 2 0 User s Guide pofu Mix Tasks Liquid Handling A Mix This task aspirates air or liquid and then dispenses it into a well a specified number of times Properties Adwanced Rtining f tmstruments For more information about the properties on each tab see Mix X Mix Properties on page A 81 Mix Advanced Rinsing on page A 83 Mix Instruments on page A 85 Mix Sequence of Steps on page A 85 A 80 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Mix Properties Property Name Brief Description Default Value Mix Zone The zone in which the mix is performed Mix Well The well in the Mix Zone in which the mix is performed When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Number of Number of times the task should repeat the commands related to mixing 1 Mixes Mix with Air Mix Select Mix with Air or
413. nse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Cherry Pick Advanced Inside Rinse Table Page 2 of 3 B 36 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Cherry Pick Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined b
414. nse the inside of the probe 500 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 1000 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Fractionate Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Fractionate Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Fractionate Advanced Rinsing Table Page 3 of 3 Fractionate Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump The pump or VPS
415. nse the inside of the probe 500 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 1000 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Wash Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide D 107 gt V gt Wash Tasks SPE D Wash Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mL min Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mL min Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Wash Advanced Rinsing Table Page 3 of 3 Wash Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump Th
416. nsfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide A 109 JaJSULAL Transfer Tasks Liquid Handling Transfer Properties Continued A Property Name Brief Description Default Value Transfer Port The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source Volume is delivered When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after
417. nside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfer Tubing volume 6 Move Z to Z Safe Height OUTSIDE RINSE Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position mM A U N gt Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume B 18 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling 6 Switch Peristaltic Rinse Pump off 7 Move Z to Z Safe Height 8 Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station TRILUTION LH Software Version 2 0 User s Guide PPV SS SS Aliquot Tasks SS Liquid Handling SS Aliquot This task dispenses the liquid source from the reservoir or tray equally to each of the wells of the result zone This task should be used in Batch mode only Properties Advanced Imide Rime Outside Rinse l tmstruments FOr more information about the properties on each tab see 6 SS Aliquot SS Aliquot Properties on page B 21 SS Aliquot Advanced Inside Rinse on page B 23 SS Aliquot Outside Rinse on pag
418. nsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Active Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Source Height The distance up when a positive number is entered or down when a negative 2 mm number is entered from a defined reference point Tube Bottom unless Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe No rinse is performed when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 10 mL min Outside Volume The quantity of liquid used to rinse the outside of the
419. nsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Dispense Random to Random with Tips Advanced Rinsing Table Page 3 of 3 Dispense Random to Random with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Dispense Random to Random with Tips Instruments TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Dispense Random to Random with Tips Sequence of Steps The following is an overview for the sequence of steps Load Tips Optional on page C 25 Aspirate on page C 25 Dispense on page C 26 Eject Tips Optional on page C 28 Inside Rinse Optional on page C 28 Outside Rinse Optional on page C 29 N OAU A U N gt Rinse Touch Off on page C 29 LOAD TIPS OPTIONAL 1 Move Z to top 2 IfLoad Tips before Task TRUE load tips ASPIRATE if Extra Volume 0 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well in Source Zone 3 Lower probe into well to Source Height 4 Prewet Tips Optional a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if sel
420. nter of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 7 If Source Volume is greater than tip volume repeat aspirate and dispense until Source Volume is delivered if Extra Volume gt 0 Move probe to Starting Well in Result Zone Lower probe into well to Result Height Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well U BR U N TRILUTION LH Software Version 2 0 User s Guide C 27 sdij 4 m WopueY 0 wopuey su ds q Dispense Random to Random with Tips Tasks Disposable Tips 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top 6 If Source Volume is greater than tip volume repeat aspirate Air Gap and Extra Volume are not aspirated and dispense until Source Volume is delivered 7 Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height 9 Dispense Extra Volume Source Air Gap at Result Flow Rate EJECT TIPS OPTIONAL If Eject Tips After Task TRUE 1 Move Z to top 2 Move probe to Tip Waste Zone 3 Eject tips by moving Z to Z Clamp Height INSIDE RINSE OPTIONAL 1 Move Z to top 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 A
421. ntered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Load Advanced Rinsing Table Page 3 of 3 Load Instruments Property Name Brief Description Default Value Pump Load Instruments The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Load Sequence of Steps The following is an overview for the sequence of steps N OW A U N gt Move Mobile Rack on page D 75 Aspirate on page D 75 Dispense on page D 77 Air Push on page D 79 Inside Rinse on page D 81 Outside Rinse on page D 81 Gas Air Purge on page D 81 MOVE MOBILE RACK 1 Move Z to top 2 Move mobile rack DEC Zone over drain ASPIRATE Reservoir 1 Move Z to top 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to DEC Well in DEC Zone 4 Lower probe to Result Height from Tube Top 5 Dispense Source Volume from Reservoir at Result Flow Rate 6 Wait Equilibration Time TRILUTION LH Software Version 2 0 User s Guide peoy Load Tasks SPE D 7 Move Z to top If Source Volume is greater than Syringe Volume repeat s
422. ntered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Condition Advanced Rinsing Table Page 3 of 3 Condition Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Condition Instruments TRILUTION LH Software Version 2 0 User s Guide D 11 UO puo gt Condition Tasks SPE D Condition Sequence of Steps The following is an overview for the sequence of steps Move Mobile Rack on page D 12 Aspirate on page D 12 Dispense on page D 14 Air Push on page D 15 Inside Rinse on page D 18 Outside Rinse on page D 18 Gas Air Purge SPE 215 Only on page D 18 N OW A WU N gt MOVE MOBILE RACK 1 Move Z to top 2 Move mobile rack DEC Zone over the drain ASPIRATE Reservoir 1 Move Z to top 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to DEC Well in DEC Zone 4 Lower probe to Result Height from Tube Top 5 Dispense Source Volume from Reservoir at Result Flow Rate 6 Wait Equilibration Time D 12 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Move Z to top If Source Volume is greater than Syringe Volume repeat steps 2 7 until Source Volume delivered 9 If SPE 215 Shake Off
423. ntered or left when a negative 0 mm Mode Side number is entered from the edge of the tube that the tip will move when X Offset performing a side Touch Off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Mix with Tips Tips Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide C 53 sdi L yum XIW Mix with Tips Tasks Disposable Tips Mix with Tips Advanced Ri
424. nto well to Result Height Dispense Source Volume Diluent Air Gap Diluent Volume at Result Flow Rate using Liquid Level Following if selected 4 Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Move probe to well in Inside Rinse Zone A 54 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling 7 8 Lower probe into well to Inside Rinse Height Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE N OW A WU N gt Move Z to Z Safe Height Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume delivered Move Z to Z Safe Height OUTSIDE RINSE N OO UW A U N gt Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height TRILUTION LH Software Version 2 0 User s Guide n q Dispense Tasks Liquid Handling A Dispense This task aspirates the specified volume of liquid source from the reservoir tray or transfer ports and then dispenses the volume to the wells of the result zo
425. oad Tips Optional on page C 12 Aspirate on page C 12 Dispense on page C 13 Eject Tips Optional on page C 15 Inside Rinse Optional on page C 15 Outside Rinse Optional on page C 16 N WOW A UN gt Rinse Touch Off on page C 16 LOAD TIPS OPTIONAL 1 Move Z to top 2 IfLoad Tips before Task TRUE load tips ASPIRATE if Extra Volume 0 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well in Source Zone 3 Lower probe into well to Source Height 4 Prewet Tips Optional a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if selected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected C 12 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips b If Prewet Tip FALSE 1 Skip to step 5 5 Aspirate Source Volume at Source Flow Rate using Liquid Level Following if selected 6 Move Z to top if Extra Volume gt 0 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move to Source Well in Source Zone 3 Lower probe into well to Source Height 4 Prewet Tips only 1st Iteration a If Prewet Tip TRUE 1 Aspirate Prewet Volume at Source Flow Rate using Liquid Level Following if selected 2 Dispense Prewet Volume at Result Flow Rate using Liquid Level Following if selected b If Prewet Tip FALSE 1 Skip to step 5 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Followin
426. obe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Rinse Probes Properties Table Page 2 of 2 Rinse Probes Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Rinse Probes Instruments TRILUTION LH Software Version 2 0 User s Guide A 105 saqojg asuly Rinse Probes Tasks Liquid Handling A Rinse Probes Sequence of Steps The following is an overview for the sequence of steps 1 Inside Rinse on page A 106 2 Outside Rinse on page A 106 INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow
427. obe at the outside rinse position in the rinse station SS Rinse Probes Outside Rinse Table Page 2 of 4 0 mm B 140 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Rinse Probes Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Rinse Probes Outside Rinse Table Page 3 of 4 TRILUTION LH
428. obe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off Move Z to Z Safe Height Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station TRILUTION LH Software Version 2 0 User s Guide B 157 Tasks Disposable Tips Gilson supplies Disposable Tips tasks for use on the Quad Z 215 with Disposable Tips TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Disposable Tips Disposable Tips Tasks Gilson Task Name and Description Cherry Pick with Tips This task transfers liquid source from wells in the source zone to a sequence of wells beginning with the starting well in the result zone Dispense Random to Random with Tips M This task transfers liquid source from wells in the source zone to random wells in the result zone Dispense with Tips This task aspirates the specified volume of liquid source from the reservoir or tray and then dispenses the volume to the wells of the result zone Eject Tips This task ejects tips to the tip waste z
429. obe to the home position 222 XL Liquid Handler Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 15 MOPpUIM qe O4 U0D jenuew Manual Control Tab Window Application Manual Control 222 XL Liquid Handler Manual Control Continued Property Brief Description Wane ief Descriptio MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration Default Value Use UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position 222 XL Liquid Handler Manual Control Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 223 Sample Changer Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 223 Sample Changer Manual Control eee Brief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number is entered or 2mm Click MOVE TO WELL down when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right of the 0 0 mm home position Click MOVE X Y Y Enter anumber to move to a position forward of the 0 0 mm home position 223 Sample Changer Man
430. od Variables To make your Method more flexible you can indicate a variable name instead of entering a value for a property in a Task A variable name begins with and can be any combination of letters and numbers and can contain some special characters Valid variable names would be FlowRate and Sample_Vol1 When setting a Sample List for a run assign values to any undefined variables used by the Method If the run is started and a value has not been assigned to a variable the following message will be displayed B Runon Q 6 8 TRILUTION LH Software Version 2 0 User s Guide Method Builder Method To see what variable names have been set for the Method and to indicate a default for each variable do the following 1 Inthe Method Builder Method tab window click the Variable List tool E 2 When the dialog box appears select the variable and then do any of the following Oi Variable List Flow Rate Zone Saw riilivity Nurnber SourceVolume Volume e To set a default value for the variable 1 Click MoDIFY or right click and then select MODIFY 2 Type a value in the Default Value box To remove an unused variable name click DELETE or right click and then select DELETE 3 Click OK when finished TRILUTION LH Software Version 2 0 User s Guide 6 9 sajqeie Method Builder Error Handling 7 TRILUTION LH can automatically respond to the occurrence of defined errors during a r
431. of 10 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of the probe For a range of 10 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 01 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 01 min Equilibration Time Evaporate Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide D 45 a ye10odeny Evaporate Tasks SPE D Evaporate Advanced Rinsing Property Name Brief Description Default Value Air Push Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delive
432. oftware Version 2 0 User s Guide Tasks Disposable Tips 3 Move probe to Surface Rinse Z Offset 4 Move Ztotop None 1 Move Z to top TRILUTION LH Software Version 2 0 User s Guide sd YIM 991d A1149 Dispense Random to Random with Tips Tasks Disposable Tips C Dispense Random to Random with Tips This task transfers liquid source from wells in the source zone to random wells in the result zone Properties tes l Advanced Rinsing Ptnstruments For more information about the properties on each tab see Dispense Random to Random with Tips oe Dispense Random to Random with Tips Properties on page C 19 Dispense Random to Random with Tips Tips on page C 20 Dispense Random to Random with Tips Advanced Rinsing on page C 22 Dispense Random to Random with Tips Instruments on page C 24 Dispense Random to Random with Tips Sequence of Steps on page C 25 C 18 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Dispense Random to Random with Tips Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn 1 Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and
433. ol eee Brief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number is entered or 2mm Click MOVE TO WELL down when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right of the 0 0 mm home position Click MOVE X Y Y Enter a number to move to a position forward of the home 0 0 mm position 222 XL Liquid Handler Manual Control Table Page 1 of 3 9 14 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 9 222 XL Liquid Handler Manual Control Continued ore Brief Description Default Value Use Name Z Enter a number to move to an absolute Z value 0 0 mm Click MOVE Z Relay Outputs 1 A filled box indicates that the circuit is closed on An Toggle the contacts 2 empty box indicates that the circuit is open off open closed off on by 3 selecting and clearing the boxes Output Contacts 4 A filled box indicates that the circuit is closed on An Toggle the contacts 5 empty box indicates that the circuit is open off open closed off on by 6 selecting and clearing 7 the boxes 8 Input Contacts 1 A filled box indicates that the circuit is closed An empty Monitor status 2 box indicates that the circuit is open 3 Buttons HOME XYZ Moves pr
434. olenoid air push before moving out of the well 0 1 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 20 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 6 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of the probe Fora range of 6 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 1 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Load Properties Table Page 4 of 5 TRILUTION LH Software Version 2 0 User s Guide D 71 peoy Load Tasks SPE D Load Properties Continued Property Name Brief Description Default Value Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 1 min Equilibration Time Load Properties Table Page 5 of 5 Load Advance
435. olume Air Gap at Result Flow Rate 11 Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Transfer Port nn A U N gt 8 9 Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume not aspirated and Dispense until Source Volume delivered If SPE 215 Shake Off TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom 10 Dispense Extra Volume Air Gap at Result Flow Rate 11 Move Z to top AIR PUSH Solenoid 1 2 Move probe to well in Inside Rinse Zone Aspirate 250 uL at 5 mL min TRILUTION LH Software Version 2 0 User s Guide uonpuo Condition Tasks SPE D 3 Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC
436. olume are not aspirated until Source Volume is delivered OV Move probe to well in Inside Rinse Zone N Lower probe into well to Inside Rinse Height 00 Dispense Extra Volume Source Air Gap at Result Flow Rate INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate 5 Dispense Inside Volume at Inside Rinse Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered 7 Move Z to Z Safe Height OUTSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe into well in Outside Rinse Zone TRILUTION LH Software Version 2 0 User s Guide A 115 JaJSULAL Transfer Tasks Liquid Handling Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OO OW fH W A 116 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B Gilson supplies SS Liquid Handling tasks for use on the following instruments GX 271 Liquid Handler with Solvent System and GX 281 Liquid Handler with Solvent System TRILUTION LH Software Version 2 0 User s Guide B 1 Tasks SS Liquid Handling B SS Liquid Handling SS Liquid Handling Tasks Gilson Task Name and Descri
437. olume are not aspirated and dispense until Source Volume is delivered 7 Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height 9 Dispense Extra Volume Source Air Gap at Result Flow Rate EJECT TIPS OPTIONAL If Eject Tips After Task TRUE 1 Move Z to top 2 Move probe to Tip Waste Zone 3 Eject tips by moving Z to Z Clamp Height INSIDE RINSE OPTIONAL 1 Move Z to top 2 Move probe to well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Flow Rate TRILUTION LH Software Version 2 0 User s Guide sd YIM 991d A1149 Cherry Pick with Tips Tasks Disposable Tips 5 Dispense Inside Volume at Inside Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered OUTSIDE RINSE OPTIONAL Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate Onn A U N gt Repeat steps 4 5 until Outside Volume delivered RINSE TOUCH OFF Side 1 Move Z to top 2 Move probe to well in Inside Rinse Zone 3 Move probe to Side Rinse Z Offset 4 Move probe 1 5 mm in the Y direction forward 5 Move probe to Side Rinse Y Offset 6 Move probe to the center of the well 7 Move Z to top Surface 1 Move Z to top 2 Move probe to well in Outside Rinse Zone C 16 TRILUTION LH S
438. olume is greater than Syringe Volume repeat steps 2 7 until Source Volume delivered 8 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top Tray 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well in Source Zone 3 Lower probe into Source Well to Source Height 4 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 5 Move Z to top Transfer Port 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well in Source Zone 3 Lower probe to Source Height from Tube Bottom 4 Set Transfer Port to On 5 Aspirate Source Volume Extra Volume at Source Flow Rate 6 Set Transfer Port to Off 7 Move Z to top D 92 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE DISPENSE Tray Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top Dispense Source Volume at Result Flow Rate Wait Equilibration Time mM A WN gt Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume delivered 6 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top 7 Move probe to well in Inside Rinse Zone 8 Lower probe to Inside Rinse Hei
439. olume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Dispense Advanced Inside Rinse Table Page 2 of 3 B 80 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Dispense Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive num
440. om the Tube Bottom ASPEC XL4 Manual Control Table Page 1 of 3 9 40 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control ASPEC XL4 Manual Control Continued ore Brief Description Default Value Use Name X Enter a number to move to a position to 0 0mm the right of the home position Click MOVE X Y Y Enter anumber to move to a position 0 0mm forward of the home position Z Enter a number to move to an absolute Z 0 0mm Click MOVE Z value Relay Outputs 1 A filled box indicates that the circuit is Toggle the contacts open closed off on 2 closed on An empty box indicates that by selecting and clearing the boxes 3 the circuit is open off Output Contacts 4 A filled box indicates that the circuit is Toggle the contacts open closed off on 5 closed on An empty box indicates that by selecting and clearing the boxes 6 the circuit is open off 7 8 ASPEC XL4 Manual Control Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 41 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control ASPEC XL4 Manual Control Continued Default Value Use ore Brief Description Name Input Contacts 1 A filled box indicates that the circuit is 2 closed An empty box indicates that the 3 circuit is open Injection Valve Monitor status
441. ommands related to mixing 1 Mixes Mix Air Gap The quantity of the air gap aspirated before the Mixing Volume 20 uL Mixing Volume The quantity of air or liquid used as part of a mixing process OuL Mixing Flow The speed at which a volume of air or liquid moves into a probe For a range of 10 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Derivatize Properties Table Page 4 of 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Liquid Level Following Derivatize Advanced Rinsing Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height Solution Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option A Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height Solution Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option
442. on 2 0 User s Guide 5 1 Method Builder Bed Layout 5 Bed Layout Tab Window The Bed Layout window is a graphical interface used to create a Bed Layout Bed Layout Tab Window aa 7 300 a i H i SISE The Method Builder Bed Layout Tab Window 5 2 TRILUTION LH Software Version 2 0 User s Guide Method Builder Bed Layout It has the following sections Section Described on Methods Palette page 5 3 Configured Instruments page 5 3 Zone Management page 5 3 Numbering Pattern page 5 6 Workspace page 5 6 Methods Palette This palette lists the methods created Configured Instruments Displays the name of the liquid handler in the Configuration and its associated bed layout Zone Management Use Zone Management to create modify delete and select zones for inactive and active wells TRILUTION LH Software Version 2 0 User s Guide 5 3 MOPUIM qe 3NoAe7 pag Bed Layout Tab Window Method Builder Bed Layout 5 4 Adding Zones 1 Click ADD ZONE Enter the name and starting number e Name When creating multiple zones each zone must have a unique name When naming zones for transfer ports name them Solvent A Solvent B etc Starting Number Identifies the first number that will be used when numbering wells in the zone 2 Use the browse buttons J to select a color or use the default colors offered Zone Properties E Name Starting
443. on 2 0 User s Guide A 57 su ds q Dispense Tasks Liquid Handling Dispense Properties Continued A Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select a Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow Rate a range of suggested flow rate values see Flow Rates on page 4 93 Dispense Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Dispense Properties Continued A
444. on designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Transfer Advanced Inside Rinse Table Page 2 of 3 B 149 TRILUTION LH Software Version 2 0 User s Guide JBJSUCAL SS SS Transfer Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Transfer Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse
445. on valve to LOAD dispenses the volume to the wells of the injection zone and then switches the injection valve to INJECT SS Mix This task aspirates air or liquid and then dispenses it into a well a specified number of times SS Liquid Handling Tasks Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide B 3 Tasks SS Liquid Handling B SS Liquid Handling Tasks Continued SS Prime Pump This task primes the transfer tubing with reservoir solvent in the result zone SS Rinse Probes _ This task rinses the inside and outside of the probe using liquid from the pump reservoir SS Transfer This task dispenses the specified volume of liquid source from the reservoir or tray to the wells of the result zone SS Liquid Handling Tasks Table Page 3 of 3 B 4 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B SS Add This task dispenses the specified volumes of liquid source from the reservoir or tray and solution from the tray to the wells of the result zone For more information about the properties on each tab see SS Add Properties on page B 6 SS Add Advanced Inside Rinse on page B 9 SS Add Outside Rinse on page B 12 SS Add Instruments on page B 15 SS Add Sequence of Steps on page B 16 TRILUTION LH Software Version 2 0 User s Guide B 5 PPV SS SS Add Tasks SS Liquid Handling SS Add Properties
446. on waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Dilute Outside Rinse Table Page 3 of 4 B 70 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dilute Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Dilute Outside Rinse Table Page 4 of 4 SS Dilute Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Dilute Outside Rinse B 71 TRILUTION LH Software Version 2 0 User s Guide 7N Ad SS SS Dilute Tasks SS Liquid Handling B SS Dilute Sequence of Steps The following is an overview for the seq
447. one Disposable Tips Tasks Table Page 1 of 3 C 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Disposable Tips Tasks Continued Load Tips IS This task loads tips Mix with Tips This task aspirates air or liquid and then dispenses it into a well a specified number of times Prime Pump with Tips This task primes the pump in the specified zone It aspirates the specified volume or the syringe capacity from the reservoir and then dispenses to the wells in the result zone Prime Rinse Station This task fills the outside rinse wells with liquid from the pump reservoir Disposable Tips Tasks Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide C 3 Tasks Disposable Tips C Disposable Tips Tasks Continued Rinse Tip Holders This task rinses the inside and outside of the tip holders Transfer with Tips This task aspirates the specified volume of liquid source from the reservoir or tray and then dispenses the volume to the wells of the result zone Disposable Tips Tasks Table Page 3 of 3 C 4 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Cherry Pick with Tips This task transfers liquid source from wells in the source zone to a sequence of wells beginning with the starting well in the result zone For more information about the properties on each tab
448. one to which to start dispensing the source volume When 1 using a multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Cherry Pick Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Cherry Pick Properties Continued Property Name Brief Description Default Value Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Cherry Pick Properties Table Page 2 of 2 Cherry Pick Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the pro
449. ottom for rinsing the probe at the outside rinse position in the rinse station Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto calculated position to which the tip will move Surface when performing a surface Rinse Touch Off Z Offset Auto calculated position is at the liquid level which is determined using the initial volume from the Sample List and the well dimensions from the Bed Layout Prime Rinse Station Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide C 69 UOI L S BSUIY BUI Prime Rinse Station Tasks Disposable Tips C Prime Rinse Station Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Prime Rinse Station Instruments Prime Rinse Station Sequence of Steps
450. ow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height N OO OW fH W TRILUTION LH Software Version 2 0 User s Guide asuadsig Dispense Random to Random Tasks Liquid Handling A Dispense Random to Random This task transfers liquid source from wells in the source zone to random wells in the result zone For more information about the properties on each tab see Dispense Random to Random Properties on page A 67 Dispense Random to Random Advanced Rinsing on page A 68 Dispense Random to Random Instruments on page A 70 Dispense Random to Random Sequence of Steps on page A 71 A 66 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Dispense Random to Random Properties A Property Name Brief Description Default Value Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where x is a value defined in the Sample List Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The
451. ow Rate inside rinse position in the rinse station SS Add Advanced Inside Rinse Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Add Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference
452. pen for 24V Outputs 1 and 2 Input A Optionally type a description for the N A N A Input B contact This description will be used to identify the contact in a Task Solenoid 1 Optionally type a description for the N A N A Solenoid 2 solenoid This description will be used to identify the solenoid in a Task General Instrument Name The name that will be used to identify Liquid Handler N A this instrument in a Task GX 271 ASPEC without Pump Properties Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 49 uoleinbiyuo e Gules Creating a Configuration Method Builder Configuration GX 271 ASPEC without Pump Properties Continued Property Name Brief Description Default Value Range Instrument Serial Number The serial number of the instrument N A N A Injection Module Serial Number The serial number of the injection N A N A module GX 271 ASPEC without Pump Properties Table Page 3 of 3 4 50 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 271 Liquid Handler The GX 271 Liquid Handler Properties Window Properties GX 271 Liquid Handler Properties Property Name Brief Description Default Value Range Setup Probe Type mm If the stop pin was inserted in S1 175 mm Probe 56 mm Probe select 56 mm Probe If the stop pin was inserted in S2 select 125 mm Probe If no stop pin was used select 175 mm Probe
453. pense Random to Random with Tips Tips Property Name Brief Description Default Value Tip Waste Zone The zone assigned to the tip chute at which the tips are ejected Load Tips Before Select and tips will be loaded at the beginning of the task Active Task Clear and tips will not be loaded If tips were previously loaded by another task clear this option Eject Tips After Select and all tips will be ejected before the rinses Active Task Clear and the tips will remain loaded Prewet Tip Select this option to wet the tip before aspirating the Source Volume 1st Iteration Cleared only Prewet Volume The quantity of liquid source aspirated and dispensed to prewet the tip when 10 uL Prewet Tip has been selected Touch Off Mode Used when Touch Off was selected on the Properties tab select Side or Surface Surface touch off Dispense Random to Random with Tips Tips Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Dispense Random to Random with Tips Tips Continued Property Name Brief Description Default Value Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Surface number is entered from an auto calculated position to which the tip will move Z Offset when performing a surface Touch Off Auto calculated position is at the liquid level which is determined using the initial volume from the Sam
454. pirate Air Gap at Air Gap Flow Rate Move Z to Z Safe Height Move probe into well in Zone Lower probe to Tube Bottom 2 mm Set Transfer Port to On Aspirate Volume at Aspirate Flow Rate Set Transfer Port to Off Move Z to Z Safe Height 10 Move probe to well in Inside Rinse Zone 11 Dispense Volume at Dispense Flow Rate e IfVolume is greater than syringe capacity repeat steps 3 11 until volume is delivered A 102 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling 12 Dispense Air Gap at Dispense Flow Rate 13 Move Z to Z Safe Height INSIDE RINSE N OW A WU N gt Move Z to Z Safe Height Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume delivered Move Z to Z Safe Height OUTSIDE RINSE N OO UW A U N gt Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height TRILUTION LH Software Version 2 0 User s Guide A 103 S Od JajsueAL BWI Rinse Probes Tasks Liquid Handling Rinse Probes This task rinses the inside and outside of th
455. plays message and allows user to Prompt for Input input a valve Message Enter the text to display in the message box Prompt Properties F 8 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Prompt Sequence of Steps 1 Display dialog box with Message text TRILUTION LH Software Version 2 0 User s Guide dwolg F 9 Run Executable Tasks Auxiliary F Run Executable This task invokes an external application wees p For more information about the properties on each tab see B Run Executable Properties on page F 10 lexe Run Executable Sequence of Steps on page F 11 Pett and Names Command Line Parameters Syechrontze O oK Cancel heip Run Executable Properties Property Name Brief Description Default Value Path and Name File path of the executable to run Command Line Optional The information sent to the program located at the Path and Name Parameters Synchronize Synchronize selected True waits for the external program to close before False continuing task execution Synchronize unselected False launches the external program and then immediately resumes task execution Run Executable Properties F 10 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Run Executable Sequence of Steps 1 Launch external program sending Command Line Parameters if entered TRILUTION LH Software Version 2 0
456. ple List and the well dimensions from the Bed Layout Touch Off The distance right when a positive number is entered or left when a negative 0 mm Mode Side number is entered from the edge of the tube that the tip will move when X Offset performing a side Touch Off Touch Off The distance up when a positive number is entered or down when a negative 0 mm Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side negative number is entered from a position that is 1 5 mm forward from the Y Offset center of the inside rinse well that the tip will move when performing a side Rinse Touch Off Dispense Random to Random with Tips Tips Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide C 21 sdij YIM Wopuey 0 wopuey ssuadsig Dispense Random to Random with Tips Tasks Disposable Tips Dispense Random to Random with Tips Tips Continued Property Name Brief Description Default Value Rinse Touch The distance up when a positive number is entered or down when a negative 0 mm Off Mode Side number is entered from the Tube Top that the tip will move when performing a Z Offset side Rinse Touch Off Rinse Touch The distance up when a positive number is entered or down when a negative 1mm Off Mode number is entered from an auto
457. point for the Source Height and Inject Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low 1 setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Inject Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide A 75 Inject Tasks Liquid Handling Inject Advanced Rinsing Continued A Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Inject Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the tube bottom when dispensing to the Injection Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The
458. porate Instruments on page D 47 Evaporate Sequence of Steps on page D 47 TRILUTION LH Software Version 2 0 User s Guide D 43 a eiodeny Evaporate Tasks SPE D Evaporate Properties Property Name Brief Description Default Value DEC Zone The zone to which the Reservoir Volume is delivered Collect Zone The zone where the evaporation will occur Collect Well The well where the evaporation will occur When using a multiple probe 1 instrument type x where x is a value defined in the Sample List SPE 215 The SPE 215 Shake Off unseals the seal foot from the mobile rack Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Solenoid Time the program waits after a solenoid air push before moving out of the well 0 01 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 20 uL Air Gap Evaporate Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Evaporate Properties Continued Property Name Brief Description Default Value Syringe The speed at which the Air Push Volume moves into the probe For a range
459. prints vials and targets Footprint A placeholder for a Rack Handle A position designated in a rack which makes the rack mobile Zones cannot be assigned to a handle Terminology Definition Method A files with a set of Tasks and a Configuration that performs actions when used in an Application Run Origin Defined by the user the location of the coordinates X 0 Y 0 Z 0 Property A Parameter Rack A Rack requires a Footprint before it can be added to a Template Examples of racks are Racks Rinse Stations Injection Port Bars Tip Boxes and Microplates Target A position defined within a well Zones can be assigned to a target Task A file with a set of Commands and Operators to perform a sequence of actions Tray A placeholder with footprints to hold the Racks Well Wells specifically custom wells can only go in Racks TRILUTION LH Software Overview 1 TRILUTION LH Software is software for controlling automated liquid handling instruments TRILUTION LH innovative features include Simple drag and drop method creation Multiple configurations and bed layouts to provide the ultimate in laboratory and application flexibility Integrated customization to create racks and tasks Graphical application simulation to ensure the functionality and reliability of the application prior to use with precious samples Easy integration with third party applications and hardware Automatic
460. probe No rinse is performed when the Outside Volume is 0 Mix with Tips Advanced Rinsing Table Page 1 of 2 0 uL C 54 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Mix with Tips Advanced Rinsing Continued Property Name Brief Description Default Value Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Mix with Tips Advanced Rinsing Table Page 2 of 2 Mix with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect Mix with Tips Instruments TRILUTION LH Software Version 2 0 User s Guide C 55 sdi L yum XIW Mix with Tips Tasks Disposable Tips C Mix with Tips Sequence of Steps The following is an overview for the sequence of steps Load
461. probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min INSIDE RINSE Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate mM A WN gt Move Z to top OUTSIDE RINSE Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Height from Tube Bottom Aspirate from Reservoir Outside Rinse Volume at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate mM A U N gt Move Z top GAS AIR PURGE If SPE 215 TRUE 1 Move Z to top 2 Set Voltage On and Output Contact 1 to Close 3 Wait 0 02 min TRILUTION LH Software Version 2 0 User s Guide D 65 9 euU01 e114 Fractionate Tasks SPE 4 Set Voltage Off and Output Contact 1 to Open 5 Move Z to top D 66 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load If analytes of interest bind to the column use this task to aspirate a specified volume of liquid source from the reservoir tray or transfer ports and
462. probe to X Coordinate or Y Coordinate If Z Coordinate TRUE e Move probe to Z Coordinate Option E 18 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Move To Zone This Task moves the probe to a specified height from bottom in a specified well in a specified zone Properties Binstruments For more information about the properties on each tab see Move To Zone Properties on page E 19 Move To Zone 7 Move To Zone Instruments on page E 20 Zone Source Zone i St no ejeje Wells 1 Height Height from bottom fmm Move To Zone Sequence of Steps on page E 20 Move To Zone Properties Property Name Brief Description Default Value Zone The zone to move to Well Enter the well number in the zone When using a multiple probe instrument type 1 x where x is a value defined in the Sample List Height Enter the height in the zone 2 Move To Zone Properties E 19 TRILUTION LH Software Version 2 0 User s Guide 9UOZ OL SAO Move To Zone Tasks Tweaks Move To Zone Instruments Property Name Default Value Liquid Handler Move To Zone Instruments Move To Zone Sequence of Steps 1 Move Z to top 2 Move probe to well in Zone 3 Lower probe into well to Height from Tube Bottom eee eceeicee E 20 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks SS Aspirate This Task aspirates liquid from th
463. probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Flow Rate Dispense Inside Volume at Inside Flow Rate Repeat steps 4 5 until Inside Volume delivered OUTSIDE RINSE OPTIONAL nun A U N gt Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate Repeat steps 4 5 until Outside Volume delivered RINSE TOUCH OFF Side Move Z to top Move probe to well in Inside Rinse Zone Move probe to Side Rinse Z Offset Move probe 1 5 mm in the Y direction forward Move probe to Side Rinse Y Offset TRILUTION LH Software Version 2 0 User s Guide C 89 sdi YUM JajsuelL Transfer with Tips Tasks Disposable Tips 6 7 Move probe to the center of the well Move Z to top Surface 1 2 3 4 Move Z to top Move probe to well in Outside Rinse Zone Move probe to Surface Rinse Z Offset Move Z to top None 1 C 90 Move Z to top TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Gilson supplies SPE tasks for use on the following instruments e ASPEC XL4 e GX 271 ASPEC GX 274 ASPEC without Pump e ASPEC XL XLi e SPE215 The Fractionate task cannot be used on the SPE 215 Default values in all tasks not optimized for the SPE 215 Additionally when using Code 300 s
464. ption SS Add ua This task dispenses the specified volumes of liquid source from the reservoir or tray and solution from the tray to the wells of the result zone SS Aliquot This task dispenses the liquid source from the reservoir or tray equally to each of the wells of the result zone This task should be used in Batch mode only SS Cherry Pick This task transfers liquid source from wells in the source zone to a sequence of wells beginning with the starting well in the result zone SS Derivatize This task dispenses the specified volumes of liquid source from the reservoir or tray and solution from the tray to the wells of the result zone and then mixes with liquid or air SS Liquid Handling Tasks Table Page 1 of 3 B 2 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B SS Liquid Handling Tasks Continued SS Dilute This task dispenses the specified volumes of liquid source from the tray and another liquid diluent from the reservoir or tray to the wells of the result zone SS Dispense This task dispenses the specified volume of liquid source from the reservoir or tray to the wells of the result zone SS Dispense Random to Random This task transfers liquid source from wells in the source zone to random wells in the result zone SS Inject This task aspirates the specified volume of liquid source switches the injecti
465. quid was detected Note If no liquid is detected the probe will go to the bottom of the tubes This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Fractionate Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide D 57 9 euU01 214 Fractionate Tasks SPE D Fractionate Advanced Rinsing Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when delivering to the DEC Zone Air Push Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to ri
466. r Configuration 402 Pump Single ID Syringe Name Size pL Reservoir Name U A A 1000 Reservoir ca The 402 Pump Single Properties Window Properties 402 Pump Single Properties as Default Property Name Brief Description Value Range Setup ID A unique identifier to address syringe onthe A A instrument Syringe Name A unique name to address syringe on the A N A instrument 402 Pump Single Properties Table Page 1 of 2 4 26 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration 402 Pump Single Properties Continued RE Default Property Name Brief Description Value Range Size uL The capacity of the left syringe A For a 1000 100 250 500 1000 5000 10000 25000 range of suggested flow rates for this syringe size see Flow Rates on page 4 93 Reservoir Name A unique name used to identify the liquid Reservoi N A that is flowing through the associated r syringe It is important for sample tracking General Instrument Name The name that will be used to identify this Pump N A instrument in a Task GSIOC ID A unique numerical address that must be 0 0 31 known to the instrument and to the software that allows the software to communicate to the instrument 402 Pump Single Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide 4 27 uoleinbiyuo e Gules Creating a Configuration Method Bui
467. r s Guide H 21 s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities H Closing Bed Layout and Utilities Do one of the following Click Z on the lower right corner to exit and return to the TRILUTION menu e Click E on the upper right corner to close the utility H 22 TRILUTION LH Software Version 2 0 User s Guide Electronic Record Management ERM Features The implementation of an Electronic Record Management ERM system can be complex TRILUTION LH has the ability to assist in attaining 21 CFR Part 11 compliance through the implementation of Electronic Record Management The ERM Features allow electronic records security and tracking audit trails electronic signatures and method versioning of records as well as permitting customized user access levels within the software This section describes how to set up and use these features For information about activating the ERM features see ERM on page 1 16 TRILUTION LH Software Version 2 0 User s Guide Electronic Record Management ERM Features Electronic Record Management ERM Features I ERM Administration ERM Features are accessed from the LIQUID HANDLING gt UTILITIES gt ERM gt ERM FEATURES menu by a user assigned to the default Admin group In this dialog box choose whether or not to enable electronic record management ERM features Check boxes default cleared thus ERM defaults disabled When Enable ERM Features
468. r Transfer Port return to Source Zone to aspirate more source if necessary If source from Reservoir aspirate more source from Reservoir if necessary Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height TRILUTION LH Software Version 2 0 User s Guide A 23 yonbily Aliquot Tasks Liquid Handling 9 Dispense Extra Volume Source Air Gap at Result Flow Rate 10 Move Z to Z Safe Height INSIDE RINSE Move Z to Z Safe Height Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Aspirate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume is delivered Move Z to Z Safe Height N OM A WU N gt OUTSIDE RINSE Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume is delivered Move Z to Z Safe Height N WO UW BWN A 24 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Cherry Pick This task transfers liquid source from wells in the source zone to a sequence of wells beginning with the starting well in the result zone Properties Vadwancedimisiog Rtetruments For more information about the propert
469. r Tubing volume 6 Move Z to Z Safe Height B 134 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B OUTSIDE RINSE mM A U N gt Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off Move Z to Z Safe Height Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station TRILUTION LH Software Version 2 0 User s Guide B 135 duing Wld SS SS Rinse Probes Tasks SS Liquid Handling SS Rinse Probes This task rinses the inside and outside of the probe using liquid from the pump reservoir For more information about the properties on each tab see SS Rinse Probes Inside Rinse on page B 137 SS Rinse Probes Outside Rinse on page B 139 SS Rinse Probes Instruments on page B 142 SS Rinse Probes Sequence of Steps on page B 143 B 136 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Rinse Probes Inside Rinse Property Name Brief Description Default
470. r rinsing the probe at the outside rinse position in the rinse station Add Advanced Rinsing Table Page 3 of 3 Add Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Add Instruments TRILUTION LH Software Version 2 0 User s Guide A 11 ppv Add Tasks Liquid Handling A Add Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 12 2 Dispense on page A 14 3 Inside Rinse on page A 14 4 Outside Rinse on page A 15 ASPIRATE Reservoir 1 Move Z to Z Safe Height 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to Solution Well in Solution Zone 4 Aspirate Solution Air Gap at Air Gap Flow Rate 5 Lower probe into well to Solution Height 6 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 7 Move Z to Z Safe Height Tray 1 Move Z to Z Safe Height 2 Move probe to Source Well in Source Zone 3 Aspirate Source Air Gap at Air Gap Flow Rate 4 Lower probe into well to Source Height A 12 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Move probe to Solution Well in Sol
471. rack define X width Y length and the height b For a circular rack define the diameter and the height The rack height is measured with respect to the point of contact between the rack and its holder For a rack placed on a template Height is measured with respect to the rack bottom For a rack placed on antlers Height is measured with respect to the underside of the rack tabs A height of 0 means that the top of the rack is level with the plane of the support This reference is important when a footprint or well is added to a custom rack the height Z is calculated with respect to this plane Also the probe height in a custom rack is calculated with respect to this plane You must be very careful when designing a custom rack that can be placed on antlers and on a tray as the probe height in tasks will be calculated with respect to this coordinate Enter the coordinates for the rack Origin The default is 0 0 0 which is the center of the rack Select the footprint s where the rack can be placed You can select several footprints 6 Click OK Adding Elements Add a single element or an array of elements TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities H Adding a single element 1 Right click on a rack and then select ADD ELEMENTS 2 Select an element Footprint Well Tip or Handle Rectan Rectan Pectin Rectan Racan Rectan Rectan Rectan Cad
472. rame 2 Click CREATE to create a new Group The Create Group window displays TRILUTION LH Software Version 2 0 User s Guide 1 23 snuaW H7 NOLLATYL TRILUTION LH Menus TRILUTION LH Software Overview 1 1 24 Bj create Group s Permissions Cwato Modify Doto Vew Ingot Epot w E e fF v F F 7 7 7 Cd od d v 7 Vv v The Create Group window 3 In the Create Group window do the following a In the Group Name field type a Group Name b In the Group Description field optional type a brief Group description c In the Permissions frame select the permissions for the various builders d Click OK The new Group displays under the Groups tab To modify a Group 1 On the Users and Groups window select a Group from the Groups tab The Users belonging to that Group display in the right frame and the MODIFY button is enabled 2 Click MoDIFY The Modify Group window displays TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Modily Group Permissions aa aaa aauag The Modify Group window 3 In the Modify Group window make the required changes and click OK To delete a Group 1 Inthe Groups tab select a Group and click the DELETE button A delete confirmation dialog box displays BJ Runon 2 Click YES The selected Group is deleted TRILUTION LH Software Version 2 0 User s Guide snuaW H7 NOILNTIYL TRILUTION LH
473. rate Inside Volume from Reservoir at Inside Rinse Flow Rate Dispense Inside Volume at Inside Rinse Flow Rate Repeat steps 4 5 until Inside Volume delivered Move Z to Z Safe Height OUTSIDE RINSE N OO UW A U N gt Move Z to Z Safe Height Move probe into well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Rinse Volume from Reservoir at Outside Rinse Flow Rate Dispense Outside Rinse Volume at Outside Rinse Flow Rate Repeat steps 4 5 until Outside Rinse Volume delivered Move Z to Z Safe Height TRILUTION LH Software Version 2 0 User s Guide dund Wld Prime Transfer Ports Tasks Liquid Handling A Prime Transfer Ports This task primes the transfer ports on ASPEC XL4 or GX 274 It aspirates the specified volume or the syringe capacity from the transfer port and then dispenses to the wells of the Inside Rinse Zone _ For more information about the properties on each tab see La Properties Rinsing Finstruments fiand Prime Transfer Ports Properties on page A 98 AS Prime Transfer Ports Rinsing on page A 100 ASPEC O GRIM Prime Transfer Ports Instruments on page A 101 Transfer Port a Prime Transfer Ports Sequence of Steps on page A 102 Volume ul b Aspirate Flow Rate mi min 10 Dispense Flow Rate mL min 10 Air Geo ul 20 A Gan FlawRate mijmink w Prime Transfer Ports Properties Property Name Brief Description Default
474. red Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Evaporate Advanced Rinsing TRILUTION LH Software Version 2 0 User s Guide Tasks SPE Property Name Liquid Handler Evaporate Instruments Brief Description The liquid handler that the Task will affect D Default Value Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor selec
475. red to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Prime Pump Outside Rinse Table Page 3 of 4 B 132 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Prime Pump Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solven
476. red at the end of the run and log information Method Report This report comprises six parts a brief summation of the method information name version description created date and created by configuration information bed layout information racks used variables steps and error handling Sample Tracking Report This report comprises a brief summation of the application run information application name and version run name run date run by and run mode and the aspirate and dispense actions for each sample TRILUTION LH Software Version 2 0 User s Guide Generating Reports 11 View a Report In the Reports window select the button that corresponds to the report to be viewed Select the parameters for the report from the drop down menu s Viewing Toolbar The toolbar provides quick access to the viewing features of the Reports window D The Export button exports the report to a file type 5 The Print button prints the report J The Refresh button refreshes the report Me a EGS The Page buttons allow the user to move between the pages of the report 2 The Go To Page option allows the user to select a specific page in the report to view Fi The Find Text option allows the user to search for a word in sub reports z The Zoom option allows the user to zoom in on or out on the report TRILUTION LH Software Version 2 0 User s Guide 11 7 yod y e M IA Export a Report Generating Reports 11 8 1
477. result zone Dispense Random to Random This task transfers liquid source from wells in the source zone to random wells in the result zone Inject This task aspirates the specified volume of liquid source switches the injection valve to LOAD dispenses the volume to the wells of the injection zone and then switches the injection valve to INJECT Mix This task aspirates air or liquid and then dispenses it into a well a specified number of times Liquid Handling Tasks Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide A 3 Tasks Liquid Handling A Liquid Handling Tasks Continued Prime 402 Dual with Tee This task primes the 402 Pump Dual with Tee in the specified zone It aspirates the full syringe capacity for each syringe and dispenses it to the wells in the result zone the specified number of cycles Prime Pump This task primes the pump in the specified zone It aspirates the specified volume or the syringe capacity from the reservoir and then dispenses to the wells in the result zone Prime Transfer Ports of This task primes the transfer ports on ASPEC XL4 or GX 274 It aspirates the specified volume or the syringe capacity from the transfer port and then dispenses to the wells of the Inside Rinse Zone Rinse Probes _ This task rinses the inside and outside of the probe using liquid from the pump reservoir Transfer This task aspirates the specifie
478. rief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Click MOVE TO WELL Z Offset The distance up when a positive number is entered ordown 2mm when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right of the home 0 0 mm position Click MOVE X Y Y Enter a number to move to a position forward of the home 0 0 mm position 215 SW Liquid Handler Manual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 9 9 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 215 SW Liquid Handler Manual Control Continued ore Brief Description Default Value Use Name Z Enter a number to move to an absolute Z value 0 0 mm Click MOVE Z Output Contacts 24V A filled box indicates that the circuit is closed on An empty Toggle the contacts 1 box indicates that the circuit is open off open closed off 2 on by selecting and 3 clearing the boxes 4 Input Contacts A A filled box indicates that the circuit is closed An empty box Monitor status B indicates that the circuit is open C D Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors an
479. rief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Elute Advanced Rinsing Table Page 3 of 3 Elute Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Elute Instruments TRILUTION LH Software Version 2 0 User s Guide D 35 any Elute Tasks SPE D Elute Sequence of Steps The following is an overview for the sequence of steps Move Mobile Rack on page D 36 Aspirate on page D 36 Dispense on page D 38 Air Push on page D 39 Inside Rinse on page D 42 Outside Rinse on page D 42 N OW A U N gt Gas Air Purge on page D 42 MOVE MOBILE RACK 1 Move Z to top 2 Move mobile rack DEC Zone over the collect position ASPIRATE Reservoir 1 Move Z to top 2 Aspirate Source Volume from Reservoir at Source Flow Rate 3 Move probe to DEC Well in DEC Zone 4 Lower probe to Result Height from Tube Top 5 Dispense Source Volume
480. rinse is performed O uL when the Inside Volume is 0 Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe No rinse is performed 0 uL when the Outside Volume is 0 Outside Flow The speed at which the Outside Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 8mm Height number is entered from the Tube Bottom for rinsing the probe at the outside rinse position in the rinse station Rinse Tip Holders Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide s1 pjoH diy asuly Rinse Tip Holders Tasks Disposable Tips Rinse Tip Holders Properties Continued Property Name Brief Description Default Value Rinse Touch The distance forward when a positive number is entered or backward whena 0 mm Off Mode Side
481. rior to a save Database Import Export Use the Database Import Export utility to make a backup copy of the database TRILUTION LH Software Version 2 0 User s Guide snual H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview Reports This menu provides the ability to generate the following report types Task Method Sample Tracking and Application For a detailed description of the reports see Reports Overview on page 11 3 About This menu displays the license information Administrative Tools 1 18 TRILUTION LH SOFTWARE CUI Handling ww Administrative Tools mm AMM TR TTT TTT 66 j ADMINISTRATIVE OOLS User Settings Change Password Users and Groups QI 208 Gwe pu rmi TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview 1 Use the Administrative Tools menu to create Users and Groups and Change Passwords The Administrative Tools menu options are e User Settings Choose whether or not TRILUTION LH should remember your user name when logging on See User Settings on page 1 19 Change Password Change passwords See Change Password on page 1 19 e Users and Groups Create and modify Users and Groups See Users and Groups on page 1 20 User Settings To have TRILUTION LH remember your user name every time you log on access the USER SETTINGS option in the Administrative Tools menu TRILUTION LH default is checked so TRILU
482. ription Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Transfer Outside Rinse Table Page 4 of 4 SS Transfer Instruments Default Value Property Name Liquid Handler Brief Description The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Transfer Instruments B 154 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Transfer Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 155 2 Dispense on page B 155 3 Inside Rinse on page B 156 4 Outside Rinse on page B 157 ASPIRATE Reservoir 1 Move Z to Z Safe Height Tray Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate 1 2 3 Move probe to Source Well in Source Zone 4 Lower probe into well to Source Height 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 6 Move Z to Z
483. roperties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Transfer with Tips Properties Continued Property Name Brief Description Default Value Reservoir The speed at which the Source Volume and Extra Volume move into the probe For 10 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each aspirate and dispense before moving out of 0 min Time the well Touch Off Select Touch Off to activate the Side or Surface Touch Off on the Tips tab Cleared Transfer with Tips Properties Table Page 2 of 2 Transfer with Tips Tips Property Name Brief Description Default Value Tip Waste Zone The zone assigned to the tip chute at which the tips are ejected Load Tips Before Select and tips will be loaded at the beginning of the task Active Task Clear and tips will not be loaded If tips were previously loaded by another task clear this option Transfer with Tips Tips Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide C 79 sdi YUM
484. ropping them into the workspace A Task is positioned anywhere in the Task Builder workspace depending on where the Task is dropped Tasks can be exported or imported across locations The new Tasks saved are listed in the Tasks palette in the Custom folder Multiple Tasks can be opened at a time TRILUTION LH Software Version 2 0 User s Guide 2 1 Task Builder Task Builder 2 Task Builder The Task Builder is a graphical user interface used to build a Task Access the Task Builder by e clicking CUSTOM TASK BUILDER from the Liquid Handling menu e right clicking a Task ina Method and then selecting OPEN TASK The Task Builder Window 2 2 TRILUTION LH Software Version 2 0 User s Guide Task Builder The Task Builder has the following sections Section Described on Tasks Palette page 2 3 Operators Palette page 2 4 Commands Palette page 2 4 Workspace page 2 4 Action Buttons page 2 8 Use the Task Builder to Create New Tasks see Creating a Task on page 2 10 Modify Existing Tasks see Modifying a Task on page 2 12 Permanently Delete Tasks see Deleting Tasks on page 2 19 Export Tasks to another location within TRILUTION see Exporting Tasks on page 2 15 Import Tasks from another location within TRILUTION see Importing Tasks on page 2 17 Tasks Palette The Tasks palette lists the saved tasks and TRILUTION LH pre defined tasks TRILUTION LH Software Version 2 0 User
485. rrently defined The file s name and path must be specified Exit Closes the Gilson GSIOC Utility software TRILUTION LH Software Version 2 0 User s Guide AINN DOISD GSIOC Utility GSIOC Utility Edit Menu There are four options in the Edit Menu Insert Immediate Insert Buffered Selection and Font Insert Immediate The Insert Immediate option in the Edit menu allows you to insert an immediate command in the command list The new command will be added to the last line in the command list The GSIOC Command will not be inserted if there is already a command line in the list with the same Unit ID and Command For more information see Insert an Immediate Command on page K 22 Insert Buffered The Insert Buffered option in the Edit menu allows you to insert a buffered command in the command list The new command will be added to the last line in the command list The GSIOC Command will not be inserted if there is already a command line in the list with the same Unit ID and Command For more information see Insert a Buffered Command on page K 23 TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility K Selection Delete Ctrl D Deletes the highlighted command from the command list Up Ctrl U Moves the highlighted command up in the command list Down Ctrl D Moves the highlighted command down in the command list Execute Ctrl E Executes the highlighted command Repeat C
486. rties On Page Ee ge Deliver with Tips Instruments on page E 14 Probe Result Volume vik 0 Result Fiawate mi jie gt Deliver with Tips Sequence of Steps on page E 15 Equilibeation Time min Deliver with Tips Properties Property Name Brief Description Default Value Result Volume The quantity of liquid delivered OuL Deliver with Tips Properties Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide E 13 sd L YUM AaAljaq Deliver with Tips Tasks Tweaks Deliver with Tips Properties Continued Property Name Brief Description Default Value Result Flow Rate The speed at which the Result Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after the dispense 0 min Time Deliver with Tips Properties Table Page 2 of 2 Deliver with Tips Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the Task will affect Pump Deliver with Tips The pump or VPS that the Task will affect If your liquid handler has an internal dilutor select the liquid handler Instruments E 14 TRILUTION LH Software Version 2 0 User s Guide Tasks Tweaks Deliver with Tips Sequence of Steps 1 Dispense Result Volume at Result Flow Rate 2 Wait Equilibration Time TRILUTION LH Software Version 2 0
487. ry Tasks SPE D AIR PUSH Solenoid Move probe to well in Inside Rinse Zone 2 Aspirate 250 uL at 5 mL min Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Ww OYO ON WD MN RA Syringe 1 Move probe to DEC Well in DEC Zone 2 Move Zto top D 24 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE CON WN HR W 9 Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top Dispense Air Push Volume at Dispense Flow Rate Wait Equilibration Time Move Z to top performing shake off SPE 215 10 Repeat steps 4 9 until Air Push Volume is dispensed 11 Move probe to Inside Rinse Wells at Inside Rinse Zone 12 Dispense Air Gap
488. s Code 20 Series Low Port Bar Large 223 Footprint Port Bar 223 Antlers Code 0 Series Rack Port Bar 223 Antlers Code 30 Series Rack Bed Layout Trays Templates 223 for Four Code 20 Series High 223 for Four Code 20 Series Low 223 for Four Deep Microplates 223 for Four Shallow Microplates Port Bar 223 Footprint Port Bar 223 Rack Rinse Station 223 Left Footprint Rinse Station 223 Lel Rinse Station 223 Right Rinse Station 223 Left G 16 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Station XL Short Left Rack TRILUTION Label Inside Rinse Drain XL Left Well 0mm rinse depth Outside Rinse XL Shallow Shallow Rinse Center Well 10 mm rinse depth Outside Deep Rinse Location Right Well Outside Rinse XL Flow Deep Rinse Right Well 45 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide suol e907 asuly Rinse Locations Rinse Locations Rinse Station 223 Tall Left Rack TRILUTION Label Inside Rinse Drain XL 0mm rinse depth Left Well Outside Rinse XL Shallow 10 mm rinse depth Shallow Rinse Center Well Outside Deep Rinse Location Right Well Outside Deep Rinse Right Well Rinse XL Deep 85 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Station 223 Right Footprint Rinse Station 223 Rig
489. s Liquid Handling A Inject This task aspirates the specified volume of liquid source switches the injection valve to LOAD dispenses the volume to the wells of the injection zone and then switches the injection valve to INJECT reneies atncdning intents For more information about the properties on each tab see Inject Inject Properties on page A 74 Inject Advanced Rinsing on page A 75 Inject Instruments on page A 77 Inject Sequence of Steps on page A 78 TRILUTION LH Software Version 2 0 User s Guide yefu Inject Tasks Liquid Handling Inject Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn When using 1 a multiple probe instrument type x where x is a value defined in the Sample List Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93
490. s Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when delivering to the DEC Zone Air Push Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 500 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 1000 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Elute Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Elute Advanced Rinsing Continued Property Name B
491. s an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Elute Properties Table Page 1 of 5 TRILUTION LH Software Version 2 0 User s Guide D 29 ain y Elute Tasks SPE D Elute Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into the probe For 3 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 1 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select the Transfer Port to activate for the task A Transfer Port Transfer Port The quantity of a sample OuL Source Volume Transfer Port An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Transfer Port The quantity of the air gap aspirated before the Source Volume 50 uL Source Air Gap Transfer Port The speed at which the Source Volume and Extra Volume move into the probe For 3 mL min Source Flow Rate a range of suggested flow rate v
492. s of lype Method F sperted Files Fee The Select Method Exported Files window 2 Onthe Select Method Exported Files window complete the following a Browse for and select the Method Export file s To select multiple Methods in a sequential order hold SHIFT any arrow key simultaneously To select Methods in a random order hold CTRL and select multiple Methods with the mouse TRILUTION LH Software Version 2 0 User s Guide Method Builder 3 b Click OPEN Some or all of the following dialogs will appear e A dialog appears with options for importing the Tasks in the Method Indicate whether to import the task as a new version rename the task import all tasks as new versions or skip the import of all tasks e A dialog appears with options for importing the Method Indicate whether to import the Method as a new version rename the Method or import all Methods as new versions e A dialog appears with options for importing the Configuration Bed Layout and Error Handler Indicate whether to rename each component overwrite the existing components or overwrite the existing components for all imported Methods e A dialog appears with an option to select a folder to save the Method to or select a folder to which all imported Methods will save A new folder can be created from this dialog by clicking NEW TRILUTION LH Software Version 2 0 User s Guide 3 11 spoy w bursodwuy Importing Methods M
493. s probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Dilute Advanced Inside Rinse Table Page 4 of 4 Omm TRILUTION LH Software Version 2 0 User s Guide B 67 7N d SS SS Dilute Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Dilute Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Zone Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe
494. s probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Mix Outside Rinse Table Page 2 of 4 B 120 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Mix Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Outp
495. s selected when aspirating the Source Volume 2mm Solution Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Solution Volume SS Add Advanced Inside Rinse Table Page 1 of 3 2mm TRILUTION LH Software Version 2 0 User s Guide B 9 PPV SS SS Add Tasks SS Liquid Handling SS Add Advanced Inside Rinse Continued Property Name Brief Description Default Value Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Fl
496. same state on error The default is No Change for Outputs 1 and 2 and Open for 24V Outputs 1 and 2 Input A Optionally type a description for the contact This N A N A Input B description will be used to identify the contactina Task GX 274 ASPEC without Pump Properties Table Page 2 of 3 4 60 TRILUTION LH Software Version 2 0 User s Guide Method Builder Configuration GX 274 ASPEC without Pump Properties Continued Property Name Brief Description Default Value Range Solenoid 1 Optionally type a description for the solenoid N A N A Solenoid 2 This description will be used to identify the solenoid in a Task General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task Instrument Serial Number The serial number of the instrument N A N A GX 274 ASPEC without Pump Properties Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide 4 61 uoleinbyuo e Gules Creating a Configuration Method Builder Configuration GX 274 Liquid Handler without Pump The GX 274 Liquid Handler without Pump Properties Window Properties GX 274 Liquid Handler without Pump Properties Property Name Brief Description Default Value Range Setup Probe Type mm If the stop pin was inserted in S1 select 56 mm 175 mm Probe 56 mm Probe Probe 125 mm Probe If the stop pin was inserted in S2 select 125 mm Probe If
497. se Zone 3 Lower probe into well to Inside Rinse Height 4 Aspirate Inside Volume from Reservoir at Inside Flow Rate 5 Dispense Inside Volume at Inside Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered OUTSIDE RINSE OPTIONAL Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate Onn A WN gt Repeat steps 4 5 until Outside Volume delivered TRILUTION LH Software Version 2 0 User s Guide s1 pjoH dil asuly Rinse Tip Holders Tasks Disposable Tips C RINSE TOUCH OFF Side 1 Move Z to top 2 Move probe to well in Inside Rinse Zone 3 Move probe to Side Rinse Z Offset 4 Move probe 1 5 mm in the Y direction forward 5 Move probe to Side Rinse Y Offset 6 Move probe to the center of the well 7 Move Z to top Surface Move Z to top Move probe to well in Outside Rinse Zone Move probe to Surface Rinse Z Offset A U N Move Z to top None 1 Move Z to top C 76 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Transfer with Tips This task aspirates the specified volume of liquid source from the reservoir or tray and then dispenses the volume to the wells of the result zone Properties Tips fAdvanced Rinsing li For more information about the properties on each tab see Transfer with Tips oo Transfer w
498. se pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Dispense Advanced Outside Rinse Table Page 3 of 4 B 84 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dispense Advanced Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent
499. see SS Cherry Pick Properties on page B 34 SS Cherry Pick Advanced Inside Rinse on page B 35 SS Cherry Pick Outside Rinse on page B 38 SS Cherry Pick Instruments on page B 41 SS Cherry Pick Sequence of Steps on page B 42 TRILUTION LH Software Version 2 0 User s Guide B 33 41d Assay SS SS Cherry Pick Tasks SS Liquid Handling SS Cherry Pick Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn 1 Solvent Valve Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Position position designated for waste cannot be used Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Source Air Gap The quantity of the air gap aspirated before the Source Volume 20 uL Source Flow The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Rate a range of suggested flow rate values see Flow Rates on page 4 93 Air Gap Flow The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4
500. selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse The zone to which the Inside Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Dispense Random to Random Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide wopuey 0 Wwopuey su ds q Dispense Random to Random Tasks Liquid Handling Dispense Random to Random Advanced Rinsing Continued A Property Name Brief Description Default Value Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside R
501. sh Volume Syringe The quantity of an air gap 20 uL Air Gap Syringe The speed at which the Air Push Volume moves into the probe For a range of 6 mL min Aspirate Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe The speed at which the Air Push Volume moves out of the probe Fora range of 6 mL min Dispense Flow suggested flow rate values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 1 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Condition Properties Table Page 4 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Condition Properties Continued Property Name Brief Description Default Value Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 1 min Equilibration Time Condition Properties Table Page 5 of 5 Condition Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down
502. side Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 250 uL Inside Flow Rate The speed at which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Derivatize Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Derivatize Advanced Rinsing Continued A Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Derivatize Advanced Rinsin
503. sion 2 0 User s Guide G 3 suol e907 asuly Rinse Locations Rinse Locations Flow Through Rinse Locations Top Wells Flow Through Rinse Locations Top Wells Rinse Station Multiple Probe 215 Inside Rinse Locations Center Trough designated as wells Rinse Station Quad Z 215 Inside Rinse Locations Center Trough designated as wells Outside Rinse Locations Bottom Wells Outside Rinse Locations Bottom Wells TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Flow Through Rinse Locations Top Wells Rinse Station Quad Z 215 with Tips Inside Rinse Locations Center Trough designated as wells Outside Rinse Locations Bottom Wells Flow Through Rinse Locations Top Wells Rinse Station SPE 215 Inside Rinse Locations Center Trough designated as wells Outside Rinse Locations Bottom Wells TRILUTION LH Software Version 2 0 User s Guide suol e907 asuly Rinse Locations Rinse Locations G 221 XL Liquid Handler Bed Layout Trays Templates Small XL Small XL Microplate Small XL Plastic Tray Port Bar Small XL Footprint Port Bar Small XL Port Bar Small XL Antlers Code 0 Series Port Bar Small XL Antlers Code 30 Series Port Bar Small XL Transfer Ports Rinse Station XL Left Footprint Rinse Station XL Left G 6 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Statio
504. sions loops if then if else and go to e Create and utilize custom tasks within TRILUTION LH to build specific and flexible applications e Understand and utilize sample lists effectively for variables and volume tracking e Create users and groups using the security functions For further information on TRILUTION LH training courses or for course registration please contact Gilson at training gilson com Fora comprehensive training calendar of courses available at Gilson Inc please visit www gilson com 1 6 TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Launching TRILUTION LH Windows 2000 When using Microsoft Windows 2000 operating system do one of the following Click START and select PROGRAMS gt GILSON APPLICATIONS gt TRILUTION LH gt TRILUTION LH On the Desktop double click the TRILUTION LH icon I The TRILUTION log on window displays TRILUTION LH gt gt f Cogon The TRILUTION Log On Window TRILUTION LH Software Version 2 0 User s Guide H7 NOILNTIYL Burysuneq Launching TRILUTION LH TRILUTION LH Software Overview 1 Windows XP When using Windows XP operating system do one of the following e Click START and select ALL PROGRAMS gt GILSON APPLICATIONS gt TRILUTION LH gt TRILUTION LH e Onthe desktop double click the TRILUTION LH icon The TRILUTION log on window displays TRILUTION LH The TRILUTION Lo
505. sitive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 500 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 1000 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Condition Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide D Tasks SPE Condition Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the Tube Bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is e
506. spirate Inside Volume from Reservoir at Inside Flow Rate C 28 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips 5 Dispense Inside Volume at Inside Flow Rate 6 Repeat steps 4 5 until Inside Volume delivered OUTSIDE RINSE OPTIONAL Move Z to top Move probe to well in Outside Rinse Zone Lower probe into well to Outside Rinse Height Aspirate Outside Volume from Reservoir at Outside Flow Rate Dispense Outside Volume at Outside Flow Rate Onn A WN Repeat steps 4 5 until Outside Volume delivered RINSE TOUCH OFF Side 1 Move Z to top 2 Move probe to well in Inside Rinse Zone 3 Move probe to Side Rinse Z Offset 4 Move probe 1 5 mm in the Y direction forward 5 Move probe to Side Rinse Y Offset 6 Move probe to the center of the well 7 Move Z to top Surface 1 Move Z to top 2 Move probe to well in Outside Rinse Zone TRILUTION LH Software Version 2 0 User s Guide C 29 sdi y IM WopueY 0 wopuey ssuadsig Dispense Random to Random with Tips Tasks Disposable Tips 3 Move probe to Surface Rinse Z Offset 4 Move Ztotop None 1 Move Zto top C 30 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Dispense with Tips This task aspirates the specified volume of liquid source from the reservoir or tray and then dispenses the volume to the wells of the result zone Properties Tips fAdvanced Rinsing li For more information about the proper
507. staltic Rinse Pump off 7 Move Z to Z Safe Height 8 Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station B 100 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Inject This task aspirates the specified volume of liquid source switches the injection valve to LOAD dispenses the volume to the wells of the injection zone and then switches the injection valve to INJECT For more information about the properties on each tab see SS Inject Properties on page B 102 SS Inject Advanced Inside Rinse on page B 103 SS Inject Advanced Outside Rinse on page B 106 SS Inject Instruments on page B 109 SS Inject Sequence of Steps on page B 110 TRILUTION LH Software Version 2 0 User s Guide B 101 p ful Ss SS Inject Tasks SS Liquid Handling SS Inject Properties Property Name Brief Description Default Value Source Zone The zone from which the Source Volume is drawn Source Well The well in the Source Zone from which the Source Volume is drawn 1 Solvent Valve Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Position position designated for waste cannot be used Source Volume The quantity of a sample OuL Extra Volume An additional volume to the Source Volume aspirated It ensures that the actual O uL volume of liquid to be transferred is not contaminated and
508. t and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection SS Dilute Advanced Inside Rinse Table Page 1 of 4 B 64 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dilute Advanced Inside Rinse Continued Property Name Brief Description Default Value Sensitivity Sensitivity is a percent 0O 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Diluent Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Diluent Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the R
509. t Flow Rate The speed at which the Result Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Touch Off Select Touch Off to move to the edge of the well after dispensing in the X direction Aliquot Properties Table Page 3 of 3 Aliquot Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Aliquot Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide A 19 yonbijy Aliquot Tasks Liquid Handling Aliquot Advanced Rinsing Continued A Property Name Brief Description Default Value Sensitivity Sensitivity is used to detect liquid when
510. t Pump Manual Control Continued Toggle the solenoids off on by selecting and clearing the boxes ore Brief Description Default Value Use Name Solenoids 1 A filled box indicates that the solenoid is 2 on An empty box indicates that the solenoid is off Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position GX 274 ASPEC without Pump Manual Control Table Page 3 of 3 TRILUTION LH Software Version 2 0 User s Guide 9 55 MOPpUIM qe O4 U0D jenuew Manual Control Tab Window Application Manual Control GX 274 Liquid Handler without Pump laterna 19 0 The GX 274 Liquid Handler without Pump Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons GX 274 Liquid Handler without Pump Manual Control rroperty Brief Description Default Value Use Name Liquid Handler Zone The zone to move to Well Enter the well number in the zone to 1 which probe A will move Z Offset The distance up when a positive number 2 mm Click MOVE TO WELL is entered or down when a negative number is entered from the Tube Bottom GX 274 Liquid Handler without Pump Manual
511. t Result Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User s Guide C 85 sdi YUM JajsuelL Transfer with Tips Tasks Disposable Tips C 6 7 b If Prewet Tip FALSE 1 Skip to step 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected Move Z to top DISPENSE Reservoir 1 2 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected If Source Volume is greater than tip volume repeat Aspirate Reservoir step 4 and Dispense Reservoir step 1 until Source Volume is delivered Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4 Move probe to center of well 5 Move Z to top b If Surface Touch Off 1 Move probe to Side Z Offset 2 Move Z to top If Source Volume is greater than tip volume repeat Aspirate and Dispense C 86 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips C Tray if Extra Volume 0 Move probe to Result Well in Result Zone Lower probe into well to Result Height Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected Dispense Source Air Gap at Result Flow Rate Wait Equilibration Time If Touch Off TRUE a If Side Touch Off 1 Move probe to Side Z Offset 2 Move probe to edge of well 3 Move probe to Side X Offset 4
512. t Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Prime Pump Outside Rinse Table Page 4 of 4 SS Prime Pump Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Prime Pump Instruments B 133 TRILUTION LH Software Version 2 0 User s Guide duing Wld SS SS Prime Pump Tasks SS Liquid Handling SS Prime Pump Sequence of Steps The following is an overview for the sequence of steps 1 Deliver on page B 134 2 Inside Rinse on page B 134 3 Outside Rinse on page B 135 DELIVER 1 Move Z to Z Safe Height 2 Move probe to Result Well in Result Zone 3 Lower probe into well to Result Height from Tube Bottom 4 Switch solvent valve to Solvent Valve Position 5 Dispense Prime Volume at Result Flow Rate 6 Move Z to Z Safe Height INSIDE RINSE Move Z to Z Safe Height Move probe to Inside Rinse Well in Inside Rinse Zone 1 2 3 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 4 Switch solvent valve to Inside Rinse Solvent Valve Position 5 Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe Inside Rinse Solvent Valve Position is not the same as the Solvent Valve Position dispense Inside Rinse Volume 2000 uL Transfe
513. t Volume x number of Result Wells at Source Flow Rate e If aliquot volume is greater than the syringe capacity aspirate the maximum syringe volume Tray Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to Source Well in Source Zone Lower probe into well to Source Height U BP WN Level Following if selected e If volume is greater than syringe capacity aspirate syringe capacity 6 Move Z to Z Safe Height A 22 Aspirate aliquot volume Result Volume x number of dispenses Extra Volume at Source Flow Rate using Liquid TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Transfer Port nn A U N gt N Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to well in Source Zone Lower probe into well to Source Height Set Transfer Port to On Aspirate aliquot volume Result Volume x number of dispenses Extra Volume at Source Flow Rate e If volume is greater than syringe capacity aspirate syringe capacity Set Transfer Port to Off Move Z to Z Safe Height DISPENSE Nn A U N gt N Move probe to Result Well in Result Zone Lower probe into well to Result Height Dispense Result Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Repeat steps 1 5 of Dispense for subsequent Result Wells If source from Tray o
514. t is aspirated and up Following while it is dispensed This selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive SS Inject Advanced Inside Rinse Table Page 1 of 3 B 103 TRILUTION LH Software Version 2 0 User s Guide p ful ss SS Inject Tasks SS Liquid Handling SS Inject Advanced Inside Rinse Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Inject Height The distance up when a positive number is entered or down when a negative 0 mm number is entered from the defined referen
515. t rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Mix Outside Rinse Table Page 4 of 4 SS Mix Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Mix Instruments B 122 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Mix Sequence of Steps The following is an overview for the sequence of steps KR WN gt MIX WITH LIQUID N WO UW A UN gt 8 9 Mix with Liquid on page B 123 Mix with Air on page B 124 Inside Rinse on page B 124 Outside Rinse on page B 125 Move Z to Z Safe Height Aspirate Air Gap at Air Gap Flow Rate Move probe to Mix Well in Mix Zone Lower probe into well to Source Height Aspirate Mix Volume at Flow Rate using Liquid Level Following if selected Lower probe into well to Result Height Dispense Mix Volume at Flow Rate using Liquid Level Following if selected Solvent Valve at Solvent Valve Position selected Wait Equilibration Time Repeat steps 4 8 until Number of Mixes is complete 10 Perform a Touch Off X direction if selected and move Z to Z Safe Height 11 Move probe to Inside Rinse Well in Inside Rinse Zone 12 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset
516. t the liquid handler Evaporate Instruments Evaporate Sequence of Steps The following is an overview for the sequence of steps mA A WN gt MOVE MOBILE RACK 1 Move Z to top Move Mobile Rack on page D 47 Air Push on page D 48 Inside Rinse on page D 50 Outside Rinse on page D 50 Gas Air Purge on page D 50 2 Move mobile rack DEC Zone over drain TRILUTION LH Software Version 2 0 User s Guide a e10deny Evaporate Tasks SPE D AIR PUSH Solenoid Move probe to well in Inside Rinse Zone 2 Aspirate 250 uL at 5 mL min Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi Wait 0 05 min SPE 215 or wait 0 01min ASPEC XL XLi Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi Move Z to top Move probe to DEC Wells in DEC Zone Lower probe to Air Push Height from Tube Top Set Voltage On and Output Contact 1 to Close SPE 215 or Set Output Contact 8 to Close ASPEC XL XLi 10 Wait Air Push Time 11 Set Voltage Off and Output Contact 1 to Open SPE 215 or set Output Contact 8 to Open ASPEC XL XLi 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense 250 uL at 5 mL min Ww OYO ON WD MN RA Syringe 1 Move probe to DEC Well in DEC Zone 2 Move Zto top D 48 TRILUTION LH Software Version 2 0 User s Guide
517. t to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments which support Liquid Level Detection Sensitivity Sensitivity is a percent 0 100 used to detect liquid when Liquid Level 1 Detection is used A low setting is the most sensitive and higher settings are less sensitive SS Cherry Pick Advanced Inside Rinse Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide B 35 Wd Y SS SS Cherry Pick Tasks SS Liquid Handling SS Cherry Pick Advanced Inside Rinse Continued Property Name Brief Description Default Value Source Height The distance up when a positive number is entered or down when a negative 2mm number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume Result Height The distance up when a positive number is entered or down when a negative 2mm number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when delivering to the Result Zone Inside Rinse Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Ri
518. t to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments that support Liquid Level Detection Sensitivity Sensitivity is used to detect liquid when Liquid Level Detection is used A low 1 setting is the most sensitive and higher settings are less sensitive Range is dependent on the instrument consult the instrument s user s guide Add Advanced Rinsing Table Page 1 of 3 TRILUTION LH Software Version 2 0 User s Guide A 9 ppv Add Tasks Liquid Handling Property Name Source Height Add Advanced Rinsing Continued Brief Description The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Source Volume A Default Value 2mm Solution Height The distance up when a positive number is entered or down when a negative number is entered from a defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when aspirating the Solution Volume 2mm Result Height The distance up when a positive number is entered or down when a negative number is entered from the defined reference point Tube Bottom unless Liquid Level Detection or Liquid Level Following is selected when deliveri
519. tations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Add Outside Rinse Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Add Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Add Outside Rinse Table Page 4 of 4 SS Add Instruments Property Name Brief Description D
520. tem during 2 Solvent Valve the Inside Rinse at the rinse station The position designated for waste cannot be Position used Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Inside Rinse The well in the Inside Rinse Zone to which the Inside Rinse Volume is delivered 1 Well Inside Rinse The quantity of liquid used to rinse the inside of the probe 1000 uL Volume Inside Rinse The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min Flow Rate inside rinse position in the rinse station SS Aliquot Advanced Inside Rinse Table Page 2 of 3 B 24 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Inside Rinse Z Option SS Aliquot Advanced Inside Rinse Continued Brief Description One of six defined reference points selected from a drop down list used when performing the inside rinse Absolute Value Moves the probe to the Z value entered if 0 is entered probe will move to the bed bottom no safety factor incorporated Auto Calculate Places probe into well and at the liquid level using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Inside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as
521. teps 2 7 until Source Volume delivered 8 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top Tray 1 Aspirate Source Air Gap at Air Gap Flow Rate 2 Move probe to Source Well in Source Zone 3 Lower probe into Source Well to Source Height 4 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 5 Move Z to top Transfer Port Aspirate Source Air Gap at Air Gap Flow Rate Move probe to well in Source Zone Lower probe to Source Height from Tube Bottom Set Transfer Port to On mM A WN gt Aspirate Source Volume Extra Volume at Source Flow Rate D 76 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE 6 Set Transfer Port to Off 7 Move Z to top DISPENSE Tray Move probe to DEC Well in DEC Zone Lower probe to Result Height from Tube Top 1 2 3 Dispense Source Volume at Result Flow Rate 4 Wait Equilibration Time 5 Move Z to top If Source Volume is greater than Syringe Volume repeat Aspirate Air Gap and Extra Volume are not aspirated and Dispense until Source Volume delivered 6 If SPE 215 TRUE perform Shake Off a Move probe to edge of well b Move probe to center of well c Move Z to top If SPE 215 FALSE a Move Z to top 7 Move probe to well in Inside Rinse Zone 8 Lower probe to Inside Rinse Height from Tube Bottom 9 Dispense Extra Volume
522. tered from the Tube Top when delivering to the DEC Zone Air Push Height The distance up when a positive number is entered or down when a negative 18 mm number is entered from the Tube Top when performing the air push Inside Rinse The zone to which the Inside Rinse Volume is delivered Inside Rinse Zone Outside Rinse Zone The zone to which the Outside Rinse Volume is delivered Outside Rinse Inside Volume The quantity of liquid used to rinse the inside of the probe 500 uL Inside Flow Rate The speed at which the Inside Rinse Volume moves out of the probe and into the 10 mL min inside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 1000 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Load Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide peoy Load Liquid Handler The liquid handler that the Task will affect Tasks SPE D Load Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is e
523. the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Prime 402 Dual with Tee Advanced Rinsing Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Liquid Handler Prime 402 Dual with Tee Instruments Brief Description The liquid handler that the task will affect A Default Value Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Prime 402 Dual with Tee Instruments Prime 402 Dual with Tee Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 91 2 Inside Rinse on page A 92 3 Outside Rinse on page A 92 ASPIRATE N WO UW A U N gt Move Z to Z Safe Height Move probe to Result Well i
524. the point of contact between the rack and its holder tray or antler 5 Specify the number of columns and the number of rows Specify the distance between wells within a column Y Offset and the distance between wells within a row X Offset 7 Optionally select a value from the Rotation drop down Default is 0 Rotation values 90 180 and 270 Click ADD Confirm that the elements were added in the Preview 9 Click CLOSE to return to the Rack TRILUTION LH Software Version 2 0 User s Guide Bed Layout and Utilities Creating a New Element Elements can be created for non standard equipment Creating a Footprint 1 2 3 4 5 Right click on a rack and then select ADD ELEMENTS Click Footprint and then NEw Enter a Footprint Name for the footprint Select the Shape Rectangle or Circle a For a circle enter the Diameter b For a rectangle enter the Length and Width Click OK Creating a Well 1 2 3 4 Right click on a rack and then select ADD ELEMENTS Click Well and then NEW Enter a Well Name for the well Select a Well Type from the drop down list TRILUTION LH Software Version 2 0 User s Guide s n pue ynoKe7 pag Bed Layout and Utilities Bed Layout and Utilities 6 Select the Shape Rectangle or Circle a For a circular well enter the Diameter Height and Maximum Volume for the well b For a rectangular well enter the Length Width Height and Maximum
525. the probe For a 0 3 mL min Air Gap Flow range of suggested flow rate values see Flow Rates on page 4 93 Rate DEC Zone The zone to which the Reservoir Volume is delivered DEC Well The well in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 min Time Reset Mobile The reset mobile rack moves the mobile rack over the drain Rack Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Load and Collect Properties Table Page 3 of 5 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D Load and Collect Properties Continued Property Name Brief Description Default Value Solenoid Time the program waits after a solenoid air push before moving out of the well 0 01 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Push Volume Syringe The quantity of an air gap 2
526. the task will affect If your liquid handler has an internal dilutor select the liquid handler Dispense Instruments Dispense Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 62 2 Dispense on page A 63 3 Inside Rinse on page A 64 4 Outside Rinse on page A 64 ASPIRATE Reservoir 1 Move Z to Z Safe Height Tray 1 Move Z to Z Safe Height 2 Aspirate Source Air Gap at Air Gap Flow Rate 3 Move probe to Source Well in Source Zone A 62 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A 4 Lower probe into well to Source Height 5 Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected 6 Move Z to Z Safe Height Transfer Port Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate Move probe to well in Source Zone Lower probe into well to Source Height Set Transfer Port to On Aspirate Source Volume Extra Volume at Source Flow Rate Set Transfer Port to Off Move Z to Z Safe Height CON AO WN RA WU Nc DISPENSE Reservoir 1 Move probe to Result Well in Result Zone 2 Lower probe into well to Result Height 3 Aspirate Source Volume from Reservoir at Source Flow Rate 4 Dispense Source Volume at Result Flow Rate using Liquid Level Following if selected If Source Volume is greater than syringe capacity repeat steps 3 4 until Source Volume is delivered Wait Equilibr
527. ties See page 9 30 819 Injection Module See page 9 32 ASPEC XL See page 9 33 ASPEC XL4 See page 9 36 ASPEC XLi See page 9 40 GX 271 ASPEC without Pump See page 9 43 GX 271 Liquid Handler See page 9 46 GX 271 Liquid Handler without Pump See page 9 50 GX 274 ASPEC without Pump See page 9 53 GX 274 Liquid Handler without Pump See page 9 56 GX 281 Liquid Handler See page 9 59 Minipuls 3 See page 9 63 Multiple Probe 215 See page 9 64 Quad Z 215 See page 9 67 SPE 215 See page 9 70 VALVEMATE See page 9 73 VALVEMATE II See page 9 74 TRILUTION LH Software Version 2 0 User s Guide 9 3 MOpUIM qe J01 Uu0 jenuew Manual Control Tab Window Application Manual Control 9 215 Liquid Handler The 215 Liquid Handler Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 215 Liquid Handler Manual Control Property Name Brief Description Default Value Use Liquid Handler Zone The zone to move to Well Enter the well number in the zone 1 Z Offset The distance up when a positive number is entered or 2 Click MOVE TO WELL down when a negative number is entered from the Tube Bottom X Enter a number to move to a position to the right of the 0 0 mm home position Click
528. ties on each tab see Dispense with Tips oo Dispense with Tips Properties on page C 32 Dispense with Tips Tips on page C 33 Dispense with Tips Advanced Rinsing on page C 36 Dispense with Tips Instruments on page C 37 Dispense with Tips Sequence of Steps on page C 38 TRILUTION LH Software Version 2 0 User s Guide C 31 sdi y4 m asuadsig Dispense with Tips Tasks Disposable Tips Dispense with Tips Properties Property Name Brief Description Default Value Source Select Reservoir or Tray Tray Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn 1 Source Well Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Reservoir The quantit
529. tinued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 5 Valid range 1 6 The position designated for Position waste cannot be used SS Aliquot Outside Rinse Table Page 4 of 4 SS Aliquot Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler SS Aliquot Instruments B 29 TRILUTION LH Software Version 2 0 User s Guide yonbijy ss SS Aliquot Tasks SS Liquid Handling SS Aliquot Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page B 30 2 Dispense on page B 31 3 Inside Rinse on page B 32 4 Outside Rinse on page B 32 ASPIRATE Reservoir 1 Move Z to Z Safe Height Tray Move Z to Z Safe Height Aspirate Source Air Gap at Air Gap Flow Rate 1 2 3 Move probe to Source Well in Source Zone 4 Lower probe into well to Source Height 5 Aspirate Aliquot Volume Result Volume x number of dispenses Ex
530. tion For a range of suggested flow rate values see Flow Rates on page 4 93 Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Transfer Advanced Rinsing Table Page 2 of 2 A 112 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Property Name Liquid Handler Transfer Instruments Brief Description The liquid handler that the task will affect A Default Value Pump The pump or VPS that the task will affect If your liquid handler has an internal dilutor select the liquid handler Transfer Instruments Transfer Sequence of Steps The following is an overview for the sequence of steps 1 Aspirate on page A 113 2 Dispense on page A 114 3 Inside Rinse on page A 115 4 Outside Rinse on page A 115 ASPIRATE Reservoir 1 Move Z to Z Safe Height Tray 1 Move Z to Z Safe Height 2 Aspirate Source Air Gap at Air Gap Flow Rate 3 Move probe to Source Well in Source Zone TRILUTION LH Software Version 2 0 User s Guide A 113 JaJSULAL Transfer Tasks Liquid Han
531. tion run and or simulation run results TRILUTION LH Software Version 2 0 User s Guide TRILUTION LH Software Overview Purge and Recover Use the Purge and Recover utility window to permanently delete files from the database and or restore deleted files back to their respective palettes It has the following buttons in the action bar e PURGE Permanently deletes the files from the database Purged files cannot be recovered e RECOVER Provides the ability to restore files back to their respective palettes Files deleted from the database cannot be recovered Close the Application window before attempting to recover an application Bed Layout and Utilities Use this utility to create custom templates and racks For more information see Appendix H Bed Layout and Utilities TRILUTION LH Software Version 2 0 User s Guide 1 15 snual H7 NOILNTIYL TRILUTION LH Menus TRILUTION LH Software Overview 1 1 16 Register Use the Registration menu to register for a licensed version of TRILUTION LH Two tabs are available e Overview Explains the registration process e Registration Information User information is entered here ERM The implementation of an Electronic Record Management ERM system can be complex TRILUTION LH has the ability to assist in attaining 21 CFR Part 11 compliance through the implementation of Electronic Record Management The ERM Features allow electronic records security
532. tra Volume at Source Flow Rate using Liquid Level Following if selected e If volume is greater than the transfer tubing aspirate as much volume as the transfer tubing will hold 6 Move Z to Z Safe Height B 30 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling DISPENSE Reservoir N OW A U N gt Move to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Result Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Repeat steps 1 6 of DISPENSE for subsequent result wells Tray Move to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Result Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Repeat steps 1 5 of DISPENSE for subsequent result wells If source from Tray return to Source Zone to aspirate more source if necessary Move probe to Inside Rinse Well in Inside Rinse Zone Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 10 Dispense Extra Volume Source Air Gap at Result Flow Rate TRILUTION LH Software Version 2 0 User s Guide B 31 onbilY SS SS Aliquot Tasks SS Liqui
533. trl R Adds or removes the repeat option for the highlighted command Font The Font option on the Edit menu allows you to change the font options for the command list fields and headers as well as the Unit ID Comment and Command text boxes TRILUTION LH Software Version 2 0 User s Guide K 17 AINN DOISD GSIOC Utility GSIOC Utility Unit ID J 29 81972 02 22 21572 50 14 506Cv3 1 2 334V1 10 1 333 1 10 Scan Auto Repeat Ghost Mode Menu There are four options in the Mode menu Scan Advanced Auto Repeat and Ghost Scan The Scan option on the Mode menu allows you to scan for GSIOC instruments After a scan the Unit ID list box displays the unit IDs and the version of the connected instruments If any connected instruments are missing from the list ensure that the proper RS 232 or GSIOC connection exists between the computer and the instruments and that the instruments do not have duplicate Unit ID numbers Advanced The advanced option in the Mode menu allows you to switch between the Basic and Advanced modes A check mark means that the GSIOC Utility is in Advanced mode TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility Auto Repeat The Auto Repeat option on the Mode menu allows you to repeat command lines that appear in the command list A command line will only repeat if a check mark appears in the Repeat field A check mark can be added by double clickin
534. trument Error Handling Method Builder Error Handling 7 4 Setting Up Instrument Error Handling 1 Select the instrument to monitor from the drop down list of instruments in the Configuration Optionally select an error method to run when this error is encountered Select the parameter from the drop down list of possible parameters for the instrument selected Select a valid mathematical operator all are listed though some would not make sense Type the value to monitor in the field to the right of the mathematical operator Optionally select the Resume Run check box Click NEW Repeat all steps for additional instruments or set up additional parameters for the same instrument Parameter Descriptions Bed Location Error This error results when a zone or well referenced in a Task or Sample List does not exist in the Bed Layout TRILUTION LH Software Version 2 0 User s Guide Method Builder Error Handling Instrument Error This error results when any scheduled command fails to execute or if TRILUTION LH loses communication with an instrument Refer to the table Error Responses on page 7 6 that describes all possible responses post error Wait Time This error results when an input contact does not occur within the length of time set on this screen If the wait time is set to zero 0 the system waits indefinitely for the input contact The valid mathematical operator is greater than or equal to
535. tside Deep Rinse Left Wells Rinse XL Flow 45 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Station XL Tall Right Rack TRILUTION Label Vemma Ss Inside Rinse Locations Right Wells Inside Rinse Drain XL Right Wells 0mm rinse depth Outside Shallow Rinse Locations Center Wells Outside Rinse XL Shallow Shallow Rinse Center Wells 10 mm rinse depth Outside Deep Rinse Locations Left Wells Outside Rinse XL Deep Deep Rinse Left Wells 85 mm wash cylinder TRILUTION LH Software Version 2 0 User s Guide suol e907 asuly Rinse Locations Rinse Locations ASPEC XL4 Bed Layout Trays Templates ASPEC XL4 Narrow Racks ASPEC XL4 Wide Racks Port Bar ASPEC XL4 Footprint Port Bar XL 4 Rack Rinse Station XL 4 Footprint Rinse Station XL 4 Rinse Station XL 4 TRILUTION LH Software Version 2 0 User s Guide Rinse Locations Rinse Station XL 4 Rack TRILUTION Label Outside Deep Rinse Location Rear Wells Inside Rinse Drain XL 0mm rinse depth Front Wells Outside Rinse XL Deep 10 mm rinse depth Deep Rinse Rear Wells TRILUTION LH Software Version 2 0 User s Guide suol e907 asuly Rinse Locations Rinse Locations G 223 Sample Changer Bed Layout Trays Templates 223 for Antlers Code 20 Series High 223 for Antler
536. ual Control Table Page 1 of 2 TRILUTION LH Software Version 2 0 User s Guide 9 17 MOPpUIM qe O4 U0 gt D enuey Manual Control Tab Window Application Manual Control 9 223 Sample Changer Manual Control Continued ore Brief Description Default Value Use Name Z Enter a number to move to an absolute Z value 0 0 mm Click MOVE Z Output Contacts 24V A filled box indicates that the circuit is closed on An Toggle the contacts open 1 empty box indicates that the circuit is open off closed off on by selecting 2 and clearing the boxes 3 4 Input Contacts A A filled box indicates that the circuit is closed An empty Monitor status B box indicates that the circuit is open C D Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors LOCK ARM Powers all motors and moves the probe to the home position 223 Sample Changer Manual Control Table Page 2 of 2 9 18 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 402 Pump Dual The 402 Pump Dual Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons Property Name Active Syringes 402 Pump Dual Manual Control Brief Descript
537. ued Property Name Brief Description LOWER SYRINGE Descends the active syringe s piston operating rod 25 of a full stroke as the syringe pump aspirates from the reservoir The valve will switch to the outlet position RAISE SYRINGE Homes the dilutor and sends the piston s to the upper position with the valve s in the probe position 444 QuadDilutor Manual Control Table Page 2 of 2 Default Value Use Used when replacing a syringe TRILUTION LH Software Version 2 0 User s Guide 9 29 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 9 506C System Interface The 506C System Interface Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons 506C System Interface Manual Control Propert ee Default y Name Brief Description Use Output Contacts 1 A filled box indicates that the circuit is closed on An Toggle the contacts open closed off 2 empty box indicates that the circuit is open off on by selecting and clearing the boxes 3 4 5 6 506C System Interface Manual Control Table Page 1 of 2 9 30 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control 506C System Interface Manual Control Continued Propert Brief Description y Name Input Contacts A A filled box indicates that t
538. uence of steps 1 Aspirate on page B 72 2 Dispense on page B 73 3 Inside Rinse on page B 74 4 Outside Rinse on page B 74 ASPIRATE Diluent Reservoir Move Z to Z Safe Height Move probe to Source Well in Source Zone Aspirate Source Air Gap at Air Gap Flow Rate Lower probe into well to Source Height Aspirate Source Volume at Source Flow Rate using Liquid Level Following if selected Move Z to Z Safe Height Onn A U N gt Diluent Tray Move Z to Z Safe Height Move probe to Source Well in Source Zone Aspirate Source Air Gap at Air Gap Flow Rate Lower probe into well to Source Height U A U N gt Aspirate Source Volume Extra Volume at Source Flow Rate using Liquid Level Following if selected B 72 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling B 6 7 8 9 Move Z to Z Safe Height Aspirate Diluent Air Gap Volume at Diluent Air Gap Flow Rate Move probe to Diluent Well in Diluent Zone Lower probe into well to Diluent Height 10 Aspirate Diluent Volume at Diluent Flow Rate using Liquid Level Following if selected 11 Move Z to Z Safe Height DISPENSE Reservoir 1 2 3 4 5 6 Move probe to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Source Volume Source Air Gap Diluent Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X dire
539. ues see Flow Rates on page 4 93 Rate DEC Zone The zone to which the Reservoir Volume is delivered DEC Well The well in the DEC Zone to which the Source Volume is delivered When using a 1 multiple probe instrument type x where x is a value defined in the Sample List Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 6 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each dispense before moving out of the well 0 1 min Time SPE 215 Shake Select the box and the SPE 215 unseals the seal foot from the mobile rack after Off dispensing or performing an air push Clear the box when an instrument other than the SPE 215 is used Air Push Select Solenoid Syringe or Valve Syringe Solenoid option only valid for the SPE 215 ASPEC XL and ASPEC XLi Valve option only valid for the GX 271 274 ASPEC and ASPEC XL4 Solenoid The length of time the air is dispensed from the solenoid or gas valve s 0 01 min Air Push Time Condition Properties Table Page 3 of 5 TRILUTION LH Software Version 2 0 User s Guide D 7 UO puo gt Condition Tasks SPE D Condition Properties Continued Property Name Brief Description Default Value Solenoid Time the program waits after a solenoid air push before moving out of the well 0 1 min Equilibration Time Syringe The quantity of air dispensed through the probe OuL Air Pu
540. uilder open CLOSE ALL Closes all open Tasks while leaving the Task Builder open TRILUTION LH Software Version 2 0 User s Guide 2 9 sapjing yseL Creating a Task Task Builder 2 10 Creating a Task Create a Task by adding Commands Tasks Variables Operators and Expressions to the workspace The drag and drop feature allows easy creation of a Task You can drag and drop Commands Tasks Variables Expressions and Operators into the workspace Saved Tasks are listed in the Tasks palette An element Task Command Expression or Operator is positioned anywhere in the Task Builder workspace depending on where the element is dropped To create a Task Do one or both of the following one or many times From the Commands palette drag a Command and then drop it in the workspace The command property page for that Command appears Review the values for each task property and modify if necessary e From the Tasks palette drag a Task and then drop it in the workspace The task property page for that Task appears Review the values for each task property and modify if necessary e From the Operators palette drag an expression variable or operator and then drop it in the workspace TRILUTION LH Software Version 2 0 User s Guide Task Builder Viewing a Task To view a task from the Task Builder do the following e Locate the task within the Tasks palette and do one of the following e Doub
541. un To make this happen set up Methods that tell TRILUTION LH what to do if it encounters one of those errors The error handling Method should not include an error handling Method and should have error limits that are greater than the control Method error limits There are four possible user defined responses for when a defined error occurs during a run A description of those responses follows e Anerror handling Method is identified and Resume Run is checked e Noerror handling Method is identified and Resume Run is checked e Anerror handling Method is identified and Resume Run is not checked e Noerror handling Method is identified and Resume Run is not checked TRILUTION LH Software Version 2 0 User s Guide 7 1 Error Handling Tab Window Method Builder Error Handling 7 2 Error Handling Tab Window The Error Handling Tab window is used to set error handling parameters D Method Guilder Untitted Mathods eine Handing Instrument Crror thar ee ie Vadbe peson If Erxilf x e m Ehe toop Doorivir The Method Builder Error Handling Tab Window TRILUTION LH Software Version 2 0 User s Guide Method Builder Error Handling It has the following sections Section Described on Setting Up Instrument Error Handling page 7 4 Modifying Instrument Error Handling page 7 8 TRILUTION LH Software Version 2 0 User s Guide 7 3 MOpuIM qe buljpuep 10113 Setting Up Ins
542. urce Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn When using 1 Source Well a multiple probe instrument type x where xis a value defined in the Sample List Tray The quantity of a sample O uL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual 0 uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume Tray The quantity of the air gap aspirated before the Source Volume 20 uL Source Air Gap Transfer Properties Table Page 1 of 3 A 108 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling Transfer Properties Continued A Property Name Brief Description Default Value Tray The speed at which the Source Volume and Extra Volume move into the probe For 5 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Source Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Transfer Port The zone from which the Source Volume is drawn Source Zone Transfer Port Select a Transfer Port to activate for the task A Transfer Port Tra
543. using initial volume from the Sample List and well dimensions from the Bed Layout accepts a positive and negative value for the Outside Rinse Z Offset Top Moves probe to the Z Safe Height accepts only a negative value for the Outside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Outside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Outside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Outside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered from a reference point defined by the Outside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Prime Pump Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide B 131 duing Wld SS SS Prime Pump Tasks SS Liquid Handling SS Prime Pump Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wi
544. ut 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used SS Mix Outside Rinse Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide B 121 XIW SS SS Mix Tasks SS Liquid Handling SS Mix Outside Rinse Continued Property Name Brief Description Default Value Rinse Well 4 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 4 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 5 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse a
545. ution Zone CON OD WM Aspirate Solution Air Gap at Air Gap Flow Rate 9 Lower probe into well to Solution Height 10 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 11 Move Z to Z Safe Height Transfer Port Move Z to Z Safe Height Move probe to well in Source Zone Aspirate Source Air Gap at Air Gap Flow Rate Lower probe into well to Source Height Set Transfer Port On Aspirate Source Volume Extra Volume at Source Flow Rate Set Transfer Port Off Move Z to Z Safe Height 9 Move probe to Solution Well in Solution Zone CON AO WM PWN c 10 Aspirate Solution Air Gap at Air Gap Flow Rate 11 Lower probe into well to Solution Height TRILUTION LH Software Version 2 0 User s Guide ppv Add Tasks Liquid Handling A 12 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 13 Move Z to Z Safe Height DISPENSE 1 Move probe to Result Well in Result Zone 2 Lower probe into well to Result Height WwW Dispense Solution Volume Solution Air Gap Source Volume at Result Flow Rate using Liquid Level Following if selected 4 Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Move probe to well in Inside Rinse Zone Dispense Extra Volume Source Air Gap Volume at Result Flow Rate 5 6 7 Lower probe into well to Inside Rinse Height 8 9 Move Z to Z Safe Height INSIDE RINSE Move Z to Z Safe He
546. utside Rinse Z Option rinsing the probe at the outside rinse position in the rinse station SS Inject Advanced Outside Rinse Table Page 2 of 4 0 mm TRILUTION LH Software Version 2 0 User s Guide B 107 p ful ss SS Inject Tasks SS Liquid Handling SS Inject Advanced Outside Rinse Continued Property Name Brief Description Default Value Peristaltic Rinse The rinse pump that will be used for the flowing outside rinse Select 1 ifthe rinse 1 Pump pump is wired to Output 1 and 24V Output 1 or select 2 if the rinse pump is wired to Output 2 and 24V Output 2 Rinse Pump Select High Low or Off OFF Speed Number of Select the number of rinse stations to be used when performing the outside rinse 1 Rinse Stations Valid range 1 5 Rinse Well 1 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 1 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 2 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 2 Valid range 1 6 The position designated for Position waste cannot be used Rinse Well 3 Desired position of the solvent selection valve on the GX Solvent System during 2 Solvent Valve the outside rinse at rinse station 3 Valid range 1 6 The position designated for Position waste cannot be used
547. values see Flow Rates on page 4 93 Rate Syringe Time the program waits after a syringe air push before moving out of the well 0 1 min Equilibration Time Valve Transfer tubing volume between the probe and the valve Volume of an air gap 4100 uL Probe to Valve aspirated to clear the transfer tubing before performing the air push The volume Volume should be less than the syringe volume Default value is the recommended value for GX 27X ASPEC Recommended value for ASPEC XL4 is 1100 uL Wash Properties Table Page 4 of 5 TRILUTION LH Software Version 2 0 User s Guide D 105 gt V gt Wash Tasks SPE D Wash Properties Continued Property Name Brief Description Default Value Valve The length of time the air is dispensed from the valve 0 01 min Air Push Time Valve Time the program waits after a valve air push before moving out of the well 0 1 min Equilibration Time Wash Properties Table Page 5 of 5 Wash Advanced Rinsing Property Name Brief Description Default Value Liquid Level When selected the probe will follow the liquid down as it is aspirated This Following selection changes the reference point for the Source Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Liquid Level When selected the probe will stop when the instrument detects the liquid Detection surface This selection changes the reference point for the Source Height to
548. ve value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from its current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Rinse Probes Inside Rinse Table Page 2 of 2 0 mm B 138 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Rinse Probes Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the outside rinse position in the rinse station SS Rinse Probes Outside Rinse Table Page 1 of 4 10 mL min TRILUTION LH Software Version 2 0 User
549. ve value for the Inside Rinse Z Offset Tube Bottom Moves the probe to the well bottom as defined by the Bed Layout accepts only positive Inside Rinse Z Offset values to move up from the bottom Tube Top Moves the probe to the top of the well as defined by the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Derivatize Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 51 9ZILALIAG SS SS Derivatize Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Derivatize Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and intothe 10 mL min outside rinse position in the rinse station SS Derivatize Outside Rinse Table Page 1 of 4 B 52
550. ve Position Rotates the valve to the selected position 2 Click PRIME 5 mL min None Inject Click SWITCH Buttons HOME XYZ Moves probe to the home position MOVE TO TOP Positions the probe at the Z Safe Height specified in the Configuration UNLOCK ARM Unpowers the X Y and Z motors GX 281 Liquid Handler Manual Control Table Page 3 of 4 TRILUTION LH Software Version 2 0 User s Guide 9 61 MOpUIM qe O4 U0 gt D jenuew Manual Control Tab Window Application Manual Control 9 GX 281 Liquid Handler Manual Control Continued Default Value Use Brief Description Property Name LOCK ARM Powers all motors and moves the probe to the home position HOME PUMP Homes the solvent system GX 281 Liquid Handler Manual Control Table Page 4 of 4 9 62 TRILUTION LH Software Version 2 0 User s Guide Application Manual Control Minipuls 3 The Minipuls 3 Manual Control Window Warning TRILUTION LH does not validate any values or check for safe movement in Manual Control Verify entries before clicking any buttons Minipuls 3 Manual Control Property Name Brief Description Default Value Prime Flow Rate The speed at which the solvent moves 10 mL min out of the probe Minipuls 3 Manual Control Use Click PRIME TRILUTION LH Software Version 2 0 User s Guide 9 63 MOpUIM qe O4 U0D jenuew Manual Control Tab Window
551. ve probe to Solution Well in Solution Zone Aspirate Solution Air Gap at Air Gap Flow Rate Lower probe into well to Solution Height 10 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 11 Move Z to Z Safe Height Transfer Port CON OWN BPWN 9 Move Z to Z Safe Height Move probe to well in Source Zone Aspirate Source Air Gap at Air Gap Flow Rate Lower probe into well to Source Height Set Transfer Port On Aspirate Source Volume Extra Volume at Source Flow Rate Set Transfer Port Off Move Z to Z Safe Height Move probe to Solution Well in Solution Zone 10 Aspirate Solution Air Gap at Air Gap Flow Rate 11 Lower probe into well to Solution Height TRILUTION LH Software Version 2 0 User s Guide aZNeALAG Derivatize Tasks Liquid Handling A 12 Aspirate Solution Volume at Solution Flow Rate using Liquid Level Following if selected 13 Move Z to Z Safe Height DISPENSE WwW CON WD MN A Move probe to Result Well in Result Zone Lower probe into well to Result Height Dispense Solution Volume Solution Air Gap Source Volume at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Move Z to Z Safe Height performing a Touch off X direction if selected Move probe to well in Inside Rinse Zone Lower probe into well to Inside Rinse Height Dispense Extra Volume Source Air Gap at Result Flow Rate MIX WITH LIQUID 1 Aspirate a Move
552. ware Version 2 0 User s Guide 2 7 sapjing yseL Task Builder Task Builder 2 8 Workspace Right click in the workspace to display the following menu options Menu PASTE Description Places a cut or copied Task in the workspace in an operator or expression or in another Method VIEW EXTENDED Hides the right panel in the workspace VIEW NORMAL Action Buttons Shows the right panel in the workspace The following action buttons are located in the lower left corner of the Task Builder Action Button Description NEW Create a new Task OPEN Open an existing Task SAVE Save an existing Task to the same name Optionally type a description and or choose a custom icon to represent the Task in the Method SAVE AS Save a new Task or save an existing Task to a new name Optionally type a description and or choose a custom icon to represent the Task in the Method TRILUTION LH Software Version 2 0 User s Guide Task Builder Action Button Description MODIFY Modify the description or custom icon for the Task DELETE Deletes the open Task or all versions of the Task EXPORT Exports selected Tasks to a specified location as LHTE files IMPORT Imports a LHTE file and lists the Task in the Tasks palettes and replaces it in the Method REFRESH Allows you to reload the Tasks palette with any new Tasks created CLOSE Closes the open Task while leaving the Task B
553. ware Version 2 0 User s Guide B 145 sajsuedL SS SS Transfer Tasks SS Liquid Handling SS Transfer Properties Property Name Brief Description Default Value Source Select Reservoir or Tray Reservoir Reservoir Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Reservoir The quantity of a sample OuL Source Volume Tray The zone from which the Source Volume is drawn Source Zone Tray The well in the Source Zone from which the Source Volume is drawn 1 Source Well Tray Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray The quantity of a sample OuL Source Volume Tray An additional volume to the Source Volume aspirated It ensures that the actual O uL Extra Volume volume of liquid to be transferred is not contaminated and acts as an extra buffer between the air gap reservoir solvent and the Source Volume The quantity of the air gap aspirated before the Source Volume 20 uL Tray Source Air Gap SS Transfer Properties Table Page 1 of 2 B 146 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Transfer Properties Continued Property Name Brief Description Default Value Tray The speed at which the Source Volume and
554. well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position mM A U N gt Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume TRILUTION LH Software Version 2 0 User s Guide B 111 p ful ss SS Inject Tasks SS Liquid Handling 6 Switch Peristaltic Rinse Pump off 7 Move Z to Z Safe Height 8 Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station B 112 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Mix This task aspirates air or liquid and then dispenses it into a well a specified number of times Properties Vadwanceitoide Rinse Ls Rise Ltestruments For more information about the properties on each tab see SS Mix Properties on page B 114 SS Mix Advanced Inside Rinse on page B 116 SS Mix Outside Rinse on page B 119 SS Mix Instruments on page B 122 SS Mix Sequence of Steps on page B 123 TRILUTION LH Software Version 2 0 User s Guide B 113 XIW SS SS Mix Tasks SS Liquid Handling SS Mix Properties Property Name Brief Description Default Value Mix Zon
555. which the Inside Volume moves out of the probe and into the inside 10 mL min rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Outside Volume The quantity of liquid used to rinse the outside of the probe 500 uL Outside Flow The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min Rate outside rinse position in the rinse station For a range of suggested flow rate values see Flow Rates on page 4 93 Cherry Pick Advanced Rinsing Table Page 2 of 3 TRILUTION LH Software Version 2 0 User s Guide Tasks Liquid Handling A Cherry Pick Advanced Rinsing Continued Property Name Brief Description Default Value Inside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the inside rinse position in the rinse station Outside Rinse The distance up when a positive number is entered or down when a negative 0 mm Height number is entered from the tube bottom for rinsing the probe at the outside rinse position in the rinse station Cherry Pick Advanced Rinsing Table Page 3 of 3 Cherry Pick Instruments Property Name Brief Description Default Value Liquid Handler The liquid handler that the task will affect Pump The pump or VPS that the task will affect If your liquid handler has an internal
556. which the Source Air Gap moves into the probe For a range of 0 3 mL min Rate suggested flow rate values see Flow Rates on page 4 93 Diluent Select Reservoir or Tray Tray Tray The zone from which the Diluent Volume is drawn Diluent Zone Tray The well in the Diluent Zone from which the Diluent Volume is drawn 1 Diluent Well Tray The quantity of liquid used to dilute the Source Volume OuL Diluent Volume SS Dilute Properties Table Page 1 of 3 B 62 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Dilute Properties Continued Property Name Brief Description Default Value Tray Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Tray The quantity of the air gap aspirated before the Diluent Volume 20 uL Diluent Air Gap Tray The speed at which the Diluent Volume moves into the probe For a range of 5 mL min Diluent Flow suggested flow rate values see Flow Rates on page 4 93 Rate Tray The speed at which the Diluent Air Gap moves into the probe For a range of 0 3 mL min Air Gap Flow suggested flow rate values see Flow Rates on page 4 93 Rate Reservoir Desired position 1 through 6 of the solvent valve on the GX Solvent System The 2 Solvent Valve position designated for waste cannot be used Position Reservoir The quantity of liquid used to dilute the
557. will be used to identify the contactina Output 2 Task Output 3 Output 4 Input A Optionally type a description for the contact This N A N A Input B description will be used to identify the contactina Input C Task Input D General Instrument Name The name that will be used to identify this Liquid Handler N A instrument in a Task GSIOC ID A unique numerical address that must be known 22 20 29 to the instrument and to the software that allows the software to communicate with the instrument 215 Liquid Handler without Pump Properties Table Page 2 of 2 4 12 TRILUTION LH Software Version 2 0 User s Guide Method Builder 215 SW Liquid Handler Configuration The 215 SW Liquid Handler Properties Window Properties 215 SW Liquid Handler Properties Property Name Brief Description Default Value Range Setup Z Clamp Height mm This number corresponds to where the mounting 270 0 285 clamp is on the vertical ruler on the installed Z arm Z Safe Height mm The Z height to which the instrument s probe 267 Calculated based on moves before moving in the X or Y direction 215 SW Liquid Handler Properties Table Page 1 of 2 Z Clamp Height Range provided on screen TRILUTION LH Software Version 2 0 User s Guide 4 13 uoleinbyuos e Nurze Creating a Configuration Method Builder Configuration 215 SW Liquid Handler Properties Continued Property Name Brief D
558. wing a Add new racks wells or zones to the template see Creating a Bed Layout on page 5 7 b Delete racks from the workspace 1 Click on the rack to select it 2 Right click and select DELETE TRILUTION LH Software Version 2 0 User s Guide 5 9 3noKe7 pag e Bulfyipoy w Modifying a Bed Layout Method Builder Bed Layout 5 10 5 c Delete wells from all zones or from the active zone by doing the following 1 Select the wells 2 Right click and select either CLEAR SELECTED WELLS FROM d Clear all zones or clear the active zone by doing the following 1 Click to select any element 2 Right click and select either CLEAR ALL ZONES or CLEAR ACTIVE ZONE e Delete zones by doing the following 1 Select the zone to be deleted 2 Right click and select DELETE f Change the zone colors see Zone Management on page 5 3 g Use the Color Management button m to modify the holder rack microplate and footprint colors h Change the numbering pattern see Numbering Pattern on page 5 6 TRILUTION LH Software Version 2 0 User s Guide Method Builder Bed Layout Deleting a Bed Layout To delete a Bed Layout do the following 1 Inthe Bed Layout utility click DELETE BED LAYOUT The Delete Bed Layout window appears Select the Bed Layout Click DELETE TRILUTION LH Software Version 2 0 User s Guide 5 11 nohe7 pag e bunajag Method Builder
559. wing if selected 11 Move Z to Z Safe Height DISPENSE Reservoir 1 2 3 4 5 6 Move to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Source Volume Solution Volume Solution Air Gap at Result Flow Rate using Liquid Level Following if selected Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height Tray 1 2 3 4 Move to Result Well in Result Zone Lower probe into well to Result Height Switch solvent valve to Solvent Valve Position Dispense Source Volume Solution Volume Solution Air Gap at Result Flow Rate using Liquid Level Following if selected TRILUTION LH Software Version 2 0 User s Guide B 17 PPV SS SS Add Tasks SS Liquid Handling B Wait Equilibration Time Perform a Touch Off X direction if selected and move Z to Z Safe Height 5 6 7 Move probe to Inside Rinse Well in Inside Rinse Zone 8 Lower probe to Inside Rinse Z Offset and Inside Rinse Z Option 9 Dispense Extra Volume Source Air Gap at Result Flow Rate using Solvent Valve at Solvent Valve Position selected INSIDE RINSE 1 Move Z to Z Safe Height 2 Move probe to Inside Rinse Well in Inside Rinse Zone 3 Lower probe into well to Inside Rinse Z Option and Inside Rinse Z Offset 4 Switch solvent valve to Inside Rinse Solvent Valve Position 5 Dispense Inside Rinse Volume at Inside Rinse Flow Rate e Ifthe I
560. witch solvent valve to Inside Rinse Solvent Valve Position 5 Dispense Inside Rinse Volume at Inside Rinse Flow Rate 6 Move Z to Z Safe Height OUTSIDE RINSE mM A WN gt Move probe to first well in Outside Rinse Zone Lower probe into well to Outside Rinse Z Option and Outside Rinse Z Offset Switch Peristaltic Rinse Pump on at Rinse Pump Speed Switch solvent valve to Rinse Well 1 Solvent Valve Position Dispense Outside Rinse Volume at Outside Rinse Flow Rate e Ifthe current solvent valve position is not the same as the previous solvent valve position dispense Outside Rinse Volume 2000 uL Transfer Tubing volume Switch Peristaltic Rinse Pump off TRILUTION LH Software Version 2 0 User s Guide B 143 S qOld SUIY SS SS Rinse Probes Tasks SS Liquid Handling 7 Move Z to Z Safe Height 8 Repeat steps 1 5 for the Number of Rinse Stations incrementing the Outside Rinse well for each rinse station B 144 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling SS Transfer This task dispenses the specified volume of liquid source from the reservoir or tray to the wells of the result zone For more information about the properties on each tab see SS Transfer Properties on page B 146 SS Transfer Advanced Inside Rinse on page B 148 SS Transfer Outside Rinse on page B 151 SS Transfer Instruments on page B 154 SS Transfer Sequence of Steps on page B 155 TRILUTION LH Soft
561. x with Air The quantity of air used as part of a mixing process O uL Mix Volume Mix with Air The quantity of an air gap aspirated before the Mix Volume 20 uL Air Gap Mix with Air The speed at which the Mix Volume moves into and out of the probe For a range 5 mL min Flow Rate of suggested flow rate values see Flow Rates on page 4 93 Mix with Air Time the program waits after each dispense before moving out of the well 0 min Equilibration Time Mix with Air Select Touch Off to move to the edge of the well after dispensing in the X Touch Off direction SS Mix Properties Table Page 2 of 2 TRILUTION LH Software Version 2 0 User s Guide B 115 XIW SS SS Mix Tasks SS Liquid Handling Property Name Liquid Level Following SS Mix Advanced Inside Rinse Brief Description When selected the probe will follow the liquid down as it is aspirated and up while it is dispensed This selection changes the reference point for the Source Height and Result Height to the liquid surface Initial Volumes should be set in the Sample List when using this option Default Value Liquid Level Detection When selected the probe will stop when the instrument detects the liquid surface This selection changes the reference point for the Source Height and Result Height to the height at which the liquid was detected Note If no liquid is detected the probe will go to the bottom of the tube This option can only be used on instruments w
562. y Advanced Butleced Le Review the Port IRQ and Baud information by selecting About from the Help menu If any information is incorrect or missing close the GSIOC Utility and use the GSIOC Configuration Editor to update the information There are four drop down menus in the Advanced mode of the Gilson GSIOC Utility File Menu Edit Menu Mode Menu and Help Menu TRILUTION LH Software Version 2 0 User s Guide K 9 GSIOC Utility GSIOC Utility Advanced Mode Buttons and Features button The Gj next to is used to insert an immediate command in the command list For more information see Insert an Immediate Command on page K 22 The G next to is used to insert a buffered command in the commandlist For more information see Insert a Buffered Command on page K 23 Arrow Up or Arrow Down button These buttons L or v are used to change the position of a command in the command list To move a command Highlight the command in the command list and use L to move the command up in the list or v to move the command down in the list TRILUTION LH Software Version 2 0 User s Guide GSIOC Utility Immediate button Sends an immediate command to the Unit ID selected The Comment field is not used Selecting the Immediate button will not add the command to the command list For more information see Send an Immediate Command on page K 24 Buffered button Sends a buffered command to the Unit
563. y Name Brief Description Default Value Output Contact This command sets the specified contact output for the specified instrument 1 Contact Status Closes and powers on the specified output contact Closed On Contact Status Opens and powers off the specified output contact Open Off Contact Status Pulses the specified output contact Default Pulse Pulse The time the program will wait between output state toggle Displays when Pulse 0 01 min Pulse Duration is selected for Contact Status Set Electrical Contact Properties Set Electrical Contact Instruments Property Name Default Value Instrument Brief Description The instrument type that the command will affect Set Electrical Contact Instruments TRILUTION LH Software Version 2 0 User s Guide F 15 332 U0D 2911 99 7 18S Set Electrical Contact Tasks Auxiliary Set Electrical Contact Sequence of Steps 1 Close Open or Pulse the specified contact F 16 TRILUTION LH Software Version 2 0 User s Guide Tasks Auxiliary Set Valve Position This task opens and powers off closes and powers on or pulses the specified contact solenoid on the specified instrument or changes the valve position on the VALVEMATE Properties OU NS Set Valve Position Properties on page F 17 Valve Type Hi Solenoid R f VALVEMATE gt Solenoid Instrument D coma fi Closed on Open off Pulse Pulse Duration min
564. y of a sample OuL Source Volume Dispense with Tips Properties Table Page 1 of 2 C 32 TRILUTION LH Software Version 2 0 User s Guide Tasks Disposable Tips Dispense with Tips Properties Continued Property Name Brief Description Default Value Reservoir The speed at which the Source Volume and Extra Volume move into the probe For 10 mL min Source Flow a range of suggested flow rate values see Flow Rates on page 4 93 Rate Result Zone The zone to which the Source Volume is delivered Result Well The well in the Result Zone to which the Source and Solution Volumes are 1 delivered Result Flow Rate The speed at which the Source Volume moves out of the probe For a range of 5 mL min suggested flow rate values see Flow Rates on page 4 93 Equilibration Time the program waits after each aspirate and dispense before moving out of 0 min Time the well Touch Off Select Touch Off to activate the Side or Surface Touch Off on the Tips tab Cleared Dispense with Tips Properties Table Page 2 of 2 Dispense with Tips Tips Property Name Brief Description Default Value Tip Waste Zone The zone assigned to the tip chute at which the tips are ejected Load Tips Before Select and tips will be loaded at the beginning of the task Active Task Clear and tips will not be loaded If tips were previously loaded by another task clear this option Dispense with Tips Tips Table Page 1 o
565. y the Bed Layout accepts a positive or negative value for the Inside Rinse Z Offset Z Adjust Moves the probe from the current position up positive value or down negative value Default Value Tube Bottom Inside Rinse Z Offset The distance up when a positive number is entered or down when a negative number is entered froma reference point defined by the Inside Rinse Z Option for rinsing the probe at the inside rinse position in the rinse station SS Cherry Pick Advanced Inside Rinse Table Page 3 of 3 Omm TRILUTION LH Software Version 2 0 User s Guide B 37 Id Y SS SS Cherry Pick Tasks SS Liquid Handling Property Name Outside Rinse Zone SS Cherry Pick Outside Rinse Brief Description The zone to which the Outside Rinse Volume is delivered Default Value Outside Rinse Outside Rinse Volume The quantity of liquid used to rinse the outside of the probe 2000 uL Outside Rinse Flow Rate The speed at which the Outside Rinse Volume moves out of the probe and into the 10 mL min outside rinse position in the rinse station SS Cherry Pick Outside Rinse Table Page 1 of 4 B 38 TRILUTION LH Software Version 2 0 User s Guide Tasks SS Liquid Handling Property Name Outside Rinse Z Option SS Cherry Pick Outside Rinse Continued Brief Description One of six defined reference points selected from a drop down list us
566. yseMm Wash Tasks SPE CON WN HR W 9 Aspirate Air Gap at Aspirate Flow Rate Move probe to DEC Well in DEC Zone Aspirate Air Push Volume at Aspirate Flow Rate Lower probe to Air Push Height from Tube Top Dispense Air Push Volume at Dispense Flow Rate Wait Equilibration Time Move Z to top performing shake off SPE 215 10 Repeat steps 4 9 until Air Push Volume is dispensed 11 Move probe to Inside Rinse Wells at Inside Rinse Zone 12 Dispense Air Gap at Dispense Flow Rate Valve CON AO MN BP WN c 9 Move probe to well in Inside Rinse Zone Aspirate Probe to Valve Volume at 10 mL min Switch valve to gas Wait 0 05 min Switch valve to probe Move Z to top Move probe to DEC Well in DEC Zone Lower probe to Air Push Height from Tube Top Switch valve to gas 10 Wait Air Push Time 11 Switch valve to probe D 114 TRILUTION LH Software Version 2 0 User s Guide Tasks SPE D 12 Wait Equilibration Time 13 Move Z to top performing shake off SPE 215 14 Move probe to well in Inside Rinse Zone 15 Dispense Probe to Valve Volume at 10 mL min INSIDE RINSE Move probe to well in Inside Rinse Zone Lower probe to Inside Rinse Height from Tube Bottom Aspirate from Reservoir Inside Rinse Volume at Inside Rinse Flow Rate Dispense Inside Rinse Volume at Inside Rinse Flow Rate mM A WN gt Move Z to top OUTSIDE RINSE Move probe to well in Outside Rinse Zone Lower probe into wells at Outside Rinse Heig

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