Minipuls 3 Peristaltic Pump User's Guide
Contents
1. Tubing Selection and Fitting Locking PY Adjustment screw Trigger ke p 99 y Compression cam r 5 Are Ns ae y HF pump heads Peristaltic tubing with retaining stops 1 Unlock each channel of the head by pressing the trigger key A towards the roller barrel 28 Set Up 2 Position the tubing around the rollers they are kept in place by the plastic stops B 3 One by one swing the compression cams back and close the locking keys Peristaltic tubing without retaining stops The peristaltic tubing is kept in place by clamps The movable retaining bar C must be fitted in such a way that the large triangular pathways are on the side of the fixed bar To fit the tubing use the same procedure as above Because the M P2 HF and MP4 HF pumps are equipped with 5 rollers rather than the 10 rollers as on the other heads pulsations may be observed at the end of the tubing Locking key ee screw Trigger key Ss Sila Mobile retaining bar Fixed bar Mobile retaining bar Fixed bar Bu pue uonoajas Buiqn 29 Set Up Tubing Selection and Fitting 30 Flow Rate Selection amp Adjustment Selection The following charts may be used to select theapproximate pump head speed rpm that is required to achieve the desired flow rate If itis possible to choose between tubings of different sizes refer to the Appendix select a middle range tubing that gives a middle range speed2. To select the mode 1 Turn off the power 2 Press the STOP key and while hold ing it turn the power on again 3 The display then shows one of three symbols to indicate the current mode A H or followed by a 2 digit identification number see Section below 4 Press the rabbit key until the symbol indicating the required mode appears 5 Press the STOP key to validate the selection Changing the Identification Number The Minipuls 3 identification ID number is factory set at 30 It could be necessary to change this ID number if several pumps communicate on the same GSIOC bus To changeit turn the power OFF Turn the power ON again while pressing the STOP key The display shows A 30 Using the and keys select the desired ID number Then press the STOP key to quit u01J9UN Y 11850 My 45 Operation Maintenance and Troubleshooting 46 Problem Blank Display Maintenance and Troubleshooting Routine maintenance The only routine maintenance required is to clean up any spilled liquids and periodically to clean the outside of the Minipuls 3 with a damp cloth Replace tubing when it shows signs of wear such as flatness or cracking Damaged tubing can cause excessive pulsations and erratic flow Troubleshooting Solution If the display is blank when the pump is turned ON proceed as follows If the fan does not run Check the power connec
3. To prepare wire for each connection cut it into pieces of appropriate length then strip about 1 cm of insulation from each end Insert each wire into the appropriate slot on the barrier strip connector Push each wire all the way in then tighten the corresponding screw Push the barrier strip connector into the socket on the Minipuls 3 The connector is designed to fit snugly into the socket Connect the other ends of the wires to the corresponding output terminals on the controlling device Make sure that the connectors are inserted correctly by referring to the diagram which indicates the function of each pair on the rear panel of the Minipuls 3 Details of how to connect a Minipuls 3 to specific Gilson Instruments are given in Chapter 5 Computer control To control the M inipuls 3 from a com puter connect an appropriate Gilson Set Up interface module or an RS232 adapter to the computer Next connect a Gilson Serial Input Output Channel GSIOC cable between the interface module or adapter and the Minipuls 3 and any other Gilson devices that are controlled from the same computer The 9 position female plug on the GSIOC cable connects directly into the male socket on the Minipuls 3 rear panel Thetype of interface module used and details of how the various units in the system are interconnected depend on the complexity of the system The user should refer to the appropriate user s guide and to C
4. pump is switched on the previously selected speed is shown on the display The pump operates at this speed when either the o or the G Key is pressed Autostart Mode This mode is indicated by a letter A to the left of the identification number In this mode the last speed and direction are stored in non volatile memory Subsequently when thepump is switched on the pump operates in the way that it did beforeit was switched off uonouny 118 SOMY 43 Operation Autostart Function 44 Hold Mode This mode is indicated by a letter H to the left of the identification number In this mode although speed and direction changes are effective at the time that they are made the non volatile memory is not updated When the pump is switched on again the pump operates in a way that is pre determined by the user regardless of any changes that were made before it was switched off To set the pre determined values that are effective when the pump is switched on proceed as follows 1 Put the pump in the Autostart mode see Mode Selection 2 Set the speed and direction wait for five seconds and then switch off 3 Switch on while pressing the STOP key then select the Hold mode using the rabbit key 4 Press the STOP key to exit the configuration program The values set in this mode are pre served until the mode is changed to either Autostart or N on autostart Operation Mode Selection
5. separate leaflet LT801354 Declaration of Conformity e eq esiuyoa 71 Trademarks Elgiloy is a registered trademark of Elgiloy Co Hastelloy is a registered trademark of Haynes MS DOS Windows are registred trademarks of Microsoft Corp Pharmed is a registre trademark of Norton Performance Plastics Corp Teflon Tefzel Viton are registre trademarks of E l du Pont deN emours amp Co Inc 72
6. without stopping the pump Pressing the RABBIT key gives the maximum speed of 48 rom in the previously selected direction 41 Operation 4 Initialization When pressed the display shows or but the previous speed is stored in memory If pressed a second time the pump rotates at the preset speed the same is applicable if either the o or the key is pressed The STOP key stops the pump and stores the displayed speed for the next start up command If the key is pressed when the pump is revolving at the RABBIT speed the last speed selected before RA BBIT selection is stored for the next start up command When the pump isremote controlled a symbol is displayed above the or sign 42 Operation Autostart Function The Autostart Function enables the pump to restart pumping at power on in a predetermined way When the power is restored the pump idles regardless of its status when the power was cut The display flashes until any key is pressed The Autostart Function has three modes as described in the next three paragraphs mode selection is described in the following paragraph Non autostart Mode This mode is indicated by a dash to the left of the identification number In this mode each timethespeed is changed the current speed is stored in non volatile memory provided no other key is pressed within three seconds of selecting the speed Subsequently when the
7. 0 3 0 5 Sleeve 0 8 od 0 6 0 8 Sleeve 1 1 od 1 2 Plastic Plastic 2 3 Plastic Set Up ie M etal sleeve connectors are used for very small bore tubing Three sizes are available from Gilson lt for connecting tubing from 0 25 mm id to 0 8 mm id Coupling Kits These are used to connect the peristaltic tubing to PTFE tubing having flanged ends or to connect the peristaltic tubing to Low Pressure Liquid Chromatogra phy LPLC systems The coupling kit consists of a connection screw and a cone The figure shows a typical connection to an LPLC system Peristaltic tubing LPLC side PTFE ml s19 dnNO pue S10J99U4U0 UOS I5H 37 Set Up Gilson Connectors and Couplers 38 A Tefzel connector is available for connecting flexible tubing id 1 to 3 mm to tubing connections screws at Gilson chromatography column outlets Couplers Two types of coupler are available Reference F1410050 consists of a set of five PVDF couplers for linking two standard Gilson connection screws 1 4 28 TPI Reference 495036 consists of one Teflon coupler for linking connection screws of different sizes 1 4 28 TPI to 10 mm standard thread Operation 4 Install the pump and make the required hydraulic and electrical connections as described in Chapter 3 Switch the pump on using the rear panel switch Then ref
8. 06 mm id F117946 Set of 12 PVC Flow tubes of 2 29 mm id F117948 Set of 12 PVC Flow tubes of 2 79 mm id F117949 Set of 12 PVC Flow tubes of 3 16 mm id Polyvinylchloride PVC Connection Tubing F117952 3 meters tubing 0 25 mm id F117953 3 meters tubing 0 38 mm id F117954 3 meters tubing 0 51 mm id F117956 3 meters tubing 0 76 mm id F117958 3 meters tubing 1 02 mm id F117960 3 meters tubing 1 30 mm id F117962 3 meters tubing 1 52 mm id F117963 3 meters tubing 1 65 mm id F117965 3 meters tubing 2 06 mm id F117966 3 meters tubing 2 29 mm id F117968 3 meters tubing 2 79 mm id F117969 3 meters tubing 3 16 mm id Polyvinylchloride PVC Connection Tubing HF For use with high flow pump heads only F117970 3 meters PVC tubing 4 mm id F117980 3 meters PVC tubing 5 mm id F117981 3 meters PVC tubing 6 mm id F117982 3 meters PVC tubing 8 mm id S9110SS9I2V jeuonippy Appendices 6 Fluoroelastomer Isoversinic Viton Tubing For use with strong acids oils aromatic and chlorinated solvents Isoversinic Calibrated Peristaltic tubing 0 40 meter lengths of Isoversinic peristaltic tubing with stops F1817741 Set of 4 Isoversinic flow tubes 0 5 mm id F1817743 Set of 4 Isoversinic flow tubes 1 0 mm id F1817745 Set of 4 Isoversinic flow tubes 2 0 mm id F117747 Set of 4 Isoversinic flow tubes 3 0 mm id F117749 Set of 4 Isoversinic flow tubes 4 0 mm id Additional Accessories Isoversinic Connection Tubing F117740 3 meters Isoversini
9. 831 Speed Control Module Model MP1 MP2 MP4 MP8 MP2 HF MP4 HF Channels 1 2 4 8 2 4 F155001 Each speed control module is supplied with the following standard accessory package Additional accessories tubing and connectors may be ordered separately 64 Appendices Standard Accessory Package Item Peristaltic tubing pack 1 x PVC tubing i d 0 4 mm for MP models 1 x PVC tubing i d 3 2 mm for MP and HF models 1 x silicone tubing i d 7 mm for HF models Qty 1 Reference F117930 Each tubing is just over 0 4 m long and is fitted with retaining stops Drain tubing Isoversinic 200 mm x 10 mmx7mm 1 F155081 Pump head screw 2 4011564204 Power cord for 220 240 V 1 7080316106 Power cord for 100 120 V 1 7080316105 Fuses type T slow blow 250 mA 5 67300240062 Fuses type T slow blow 500 mA 5 6730054006 Terminal block 6 pin 1 638306512 User s Guide 1 LT801121 S9110SS9IIY PUB SISQUINN Med 65 Appendices Additional Accessories 66 Additional Accessories Additional accessories such as tubing and connectors can be supplied by Gilson These items are described in Chapter 3 and listed below Connectors Metal Sleeve Connectors for Small Bore Tubing F117985 Set of 5 Stainless steel connectors 0 6 mm od F117986 Set of 5 Stainless steel connectors 0 8 mm od F117987 Set of 5 Stainless steel connectors 1 1 mm od Plastic Connectors for Large Bore Tub
10. Adjustment Adjust the cam pressure on the tubing to the minimum necessary to ensure pumping of the liquid The compression cam pressure can be adjusted using the adjustment screw Slowly tighten the screw until the pump starts pumping liquid inside the peristaltic tubing and then tighten again approximately 1 8 turn Take care not to over tighten the screws it is advisable to limit pressure on tubing to the minimum necessary for pumping liquid Carry out final adjustment after 15 minutes of operation If the pump is used with a system that provides a back pressure such as a chromatographic column tighten the screw by one or two extra turns to obtain the required flow rate Set Up Flow mL min 0 5 Speed Flow rate Chart Small Sized Tubing 2 MP Model Small Sized Tubing S eS se se S KS AY w 1 5 we SS en MS 1 0 5 pvco 2 mm 1 7 r r 0 10 20 30 40 50 Pump Head Speed RPM Speed Flow rate Chart Medium Sized Tubing 30 MP Model Medium Sized Tubing F 25 F 20 10 20 30 40 50 Pump Head Speed RPM Pu pue uon991as Buiqny 31 Set Up 3 Speed Flow rate Chart Large Sized Tubing 300 300 HF Model Large Sized Tubing Tubing Selection and Fitting E a 3 a 0 10 20 30 40 50 Pump Head Speed RPM Speed Flow rate Chart Fluoroelastomer Sized Tubing 40 eS 40 HF Model Fluoroelastom
11. Intermediate Keypad and Remote Request keypad status og c is the code of the last key pressed s is the key s status c one of the following lt backwards CCW gt forwards CW faster slower H stop 8 rabbit none only at power on s one of the following l if the key was pressed after the last request if the key remains pressed after the last request a space means that no key was pressed g R Buffered Remote Set speed Rrrrr rrrr is the new speed in hundredths of a revolution per minute ranging between 0 and 4800 An input of R is interpreted as RO R Immediate Keypad and Remote Read Display dXX XXca d is the direction status a space if stopped if CW if COW XX XX is the speed status in rom at full speed rabbit c is the control status K if started through the keypad R if started through the contacts or set to remote mode through GSIOC a is the autostart condition if in autostart condition display flashing a space if not in autostart 12 50K when the pump is new s Remote Control Command Type Mode Function Syntax Parameter Command Type Mode Function Response format Where Default response Examples Example 1 Ss Buffered Keypad and Remote Set External C
12. Minipuls 3 Peristaltic Pump User s Guide GILSON SOLUTIONS AT WORK FOR YOU LT801121 M O 2003 Gilson SAS All rights reserved February 2003 Table of Contents Introduction Unpacking Description Introduction Speed Control Module Pump Head Back Panel Membrane Keypad Assembly Electrical Changing the Pump Head Tubing Selection and Fitting Recommendations Gilson Connectors and Couplers Operation Keypad Functions Initialization Autostart Function Maintenance and Troubleshooting Remote Control Electrical Contact Control Control by aGilson Fraction Collector Control by a Gilson Sampler Microcomputer Control GSIOC Appendices Part Numbers and Accessories Additional Accessories Technical Data Introduction 1 This U ser s Guide describes how to set up and operate a Gilson Minipuls 3 Peristaltic Pump It also describes some of the meth odology required to obtain accurate reproducible results The user must know how to set up and operate a Minipuls 3 before the unit is used therefore Chapters 1 to 4 including the safety notes must be read first The instrument is designed to be used only by properly qualified and trained personnel Chapter 1 contains general information including the specifica tions and safety notes Chapter 2 contains a detailed description of the hardware Chapter 3 describes how to set up a pump and details the recom mended connectors and tubing Chapter 4 describes how to ope
13. R 2 vx l l l l B l B l B l Command Descriptions Below is thelist of available commands in alphabetical order This list may be used as a quick reference guide A detailed description of each command is given after the table Mode Function Keypad and remote Request Module Identification Keypad and remote Request Mode Status Keypad and remote Master Reset Keypad and remote Request Contact Input Status Remote Input Remote Keystroke Keypad and remote Request Keypad Status Remote Set Speed Keypad and remote Read Display Keypad and remote Set External Mode Keypad and remote Request Analog Input Status The default response given in some of the following descriptions is the response returned by the Minipuls 3 when an immediate command is sent at power on or after a master reset The response to a buffered command isaperiod Because the controller command buffer is 40 characters long buffered command strings can be as long as 39 characters the last character being the CR Carriage Return ASCII code Remote Control 5 Command Type Immediate Mode Keypad and Remote Function Module identification Response format 312Vx y where Vx y identifies the software version Command Type Immediate Mode Keypad and Remote Function Request Mode Status Response format K for keypad R for remote 9OISD 1011409 13 nNdwo30191W Default response K Command Type Immediate M
14. The pin out is shown below 0 5 V giving 0 100 INPUT 0 5 V 7 of the selected speed START 0 V Active Start TOP 4 Start Stop I 5 v Inactive Stop et i 0 V Active CCW Direction Y L Control S MN l 5 V Inactive CW Start Stop Input Contact Input The pump can be started by closing this input and stopped by opening it The input is dosed by shorting pin 3 to ground pin 2 4 or 6 Closing this input starts the pump in the direction selected by the Direction Control input at the speed defined by the Analog Input Remote Control When the pump is started using this input the keypad has no effect until the Start Stop contact is opened again Direction Control Contact Input This input only works when the Start Stop input is activated When the Direction Control contact is opened the pump rotates in the forward direction dockwise When this contact is dosed the pump rotates in the reverse direction counter clockwise The direction of rotation may be changed while the pump is running in which case the change is immediate Pump Speed Analog Input This input only works when the Start Stop input is activated A DC voltage Oto 5 Volts or a resistance resistor or potentiometer between pin 5 and ground can be used to change the rotation speed as described below V oltage control Effective Speed Selected Speed x Applied Voltage 5 Volts TheA pplie
15. c tubing 0 5 mm id F117742 3 meters Isoversinic tubing 1 0 mm id F117744 3 meters Isoversinic tubing 2 0 mm id F117746 3 meters Isoversinic tubing 3 0 mm id F117748 3 meters Isoversinic tubing 4 0 mm id F117750 3 meters Isoversinic tubing 6 0 mm id Silicone Tubing Silicone Calibrated Peristaltic tubing 0 40 meter lengths of Silicone peristaltic tubing with stops F1825111 Set of 4 Silicone flow tubes 0 6 mm id F1825112 Set of 4 Silicone flow tubes 1 0 mm id F1825113 Set of 4 Silicone flow tubes 2 0 mm id F1825114 Set of 4 Silicone flow tubes 2 8 mm id Silicone Connection Tubing F1825131 3 meters Silicone tubing 0 6 mm id F1825132 3 meters Silicone tubing 1 mm id F1825133 3 meters Silicone tubing 2 mm id F117975 3 meters Silicone tubing 3 mm id F117976 3 meters Silicone tubing 4 mm id F117977 3 meters Silicone tubing 5 mm id F117978 3 meters Silicone tubing 6 mm id F117979 3 meters Silicone tubing 7 mm id 68 Appendices Polypropylene Pharmed Tubing For usein the pharmaceutical and food industries Polypropylene Calibrated Peristaltic Tubing 0 4 meter lengths of peristaltic tubing with stops F1825101 Set of 4 Polypropylene Flow tubes of 0 5 mm id F1825102 Set of 4 Polypropylene Flow tubes of 1 0 mm id F1825103 Set of 4 Polypropylene Flow tubes of 2 0 mm id Polypropylene Connection Tubing F1825121 3 meters Polypropylene tubing 0 5 mm F1825122 3 meters Polypropylene tubing 1 0 mm F1825123 3 meters Polypropylen
16. d Voltageis expressed in Volts The Selected Speed is displayed before the Start Stop input is activated The Effective Speed is displayed after the Start Stop input is activated 101 u09 98 U09 291119913 49 Remote Control Electrical Contact Control 50 Resistance control Effective Speed Reference Speed x Resistance Resistance 68 The resistance is expressed in kOhms The Reference Speed is obtained with an infinite resistance open circuit Remote Control Control by a Gilson Fraction Collector Connection to a fraction collector is mainly for the purpose of turning off the pump after collection The next two sections describe how to make the electrical connections Using an FC202 to Control a Minipuls 3 Make the following connections be tween the FC202 and the Minipuls Trlisan NPN transistor for example a type BC107 transistor Resistor R1 should have a value of between 5 kQ and 680 KQ to use the fraction collec tor s RUNNING signal properly The Minipuls 3 stops during the INJECT stage during PAUSE and at the end of the collection process 202 Barrier Strip Minipuls 3 Barrier Strip Connector Pin pl 15 Tri 5 V O Pin 3 Stop Run Pin 25 Running O O Pin 4 Ground Pin 1 Ground 10 99 0D UO0 98 14 UOS I e Aq O1JUO 51 Remote Control Control by a Gilson Fraction Collector 52 Using an FC203 204 to Control a Minipuls 3 Maket
17. ded Tubing by Application Material PVC Silicone Polypropylene Isoversinic Viton Composition Polyvinyl chloride Silicone elastomer Polypropylene based Fluorinated elastomer material with plasticizer Physical Transparent clear Translucent beige Opaque beige Opaque black Characteristics rigid Temperature range Up to 94 C Up to 230 C 60 to 135 C 20 to 200 C Standard FDA food quality FDA 21 CFR 177 26000 USP class VI Gas Permeability Low High Low Impermeable to most gases Sterilization Autoclaving Autoclaving or irridiation Autoclaving Autoclaving Range of internal diameter 0 25 to 8 mm 0 64 to 7 mm 0 5 to 6 4 mm 0 5 to 6 0 mm Other features Low cost Excellent biocompatibility Long service life Excellent resistance to Not recommended with strong acids oxidizing strong acids bases or agents aromatic and solvents chlorinated solvents Applications General use Biological Pharmaceutical Where high chemical and food resistance is required e g ICP 27 Set Up 3 Fitting the Tubing MP pump heads Only peristaltic tubing with retaining stops can be fitted to MP pump heads 1 Unlock each channel of the head by pressing the trigger key A towards the roller barrel 2 Position the tubing around the rollers itiskeptin place by theplasticstops B 3 Swing the compression cam back and snap the locking key Do this one by one for pump heads with more than one channel
18. ds are composed of a rotor with idle stainless steel rollers that press a flexible piece of tubing against acam In this way liquids are pumped by a peristaltic effect There are two types of pump heads the MP standard and the HF High Flow series Models of the standard series have one two four or eight channels ten rollers and accept tubing size from 0 2 mm to 4mm internal diameter The maximum back pressure is 500 kPa 75 PSI HF models have two or four channels five rollers and accept peristaltic tubing from 2 mm to 8mm because of the V clamp locking system The maximum back pressure is 300 kPa 45 PSI Adjustment screw ae ee Trigger key Fj M A 1 ry EF Description Locking ae Mobile retaining bar Fixed bar Adjustment screw Mobile retaining bar Trigger key Fixed bar peay duing 13 Description 2 Back Panel The back panel holds Back Panel apower cord socket and a power switch with fuses e afan e an input output control socket e a9pin GSIOC socket Input Output control GSIOC control Power switch Voltage selection 14 Description Membrane Keypad Assembly The membrane keypad assembly is integrated with the main housing The keypad and liquid crystal display are shown in the figure Keypad jquiassy pedAsy sueiquoyy LCD Symbol Function Description Forwards Start the pump clockwise Bac
19. e tubing 2 0 mm F1825124 3 meters Polypropylene tubing 3 2 mm F1825125 3 meters Polypropylene tubing 6 4 mm Electrical Connector 38306512 6 pin barrier strip connector s oss 29y jeuolppy 69 Appendices Technical Data 70 Technical Data O perating Temperature 4 40 C No warm up time is required before operation Position Upright only vertical 5 H ead Speed 0 01 to 48 rpm Torque Greater than 3 Nm at any speed below 25 rpm at or above the nominal voltage 110V or 220V M otor Speed Stability 0 5 for any variation of line voltage torque or temperature Continuous Speed Adjustment From 0 to 48 rpm by 0 01 increments up to 9 99 rpm above 9 99 rpm by 0 1 increments Flow Rate Range Maximum MP model 40mL min 18L h 4 mm tubing HF model 250 mL min 15 L h 8 mmtubing Minimum 0 3 uL min 0 25 mm tubing M aximum Back Pressure 500 kPa for MP models 300 kPa for HF models Peristaltic Tubing Diameter 0 25to 4mm id for MP models 2 to 8 mm id for HF models Appendices Line Voltage 90V to 132V for 110 voltage selection 180V to 264V for 220 voltage selection Line Frequency 47 to 63 Hz Power Consumption Full load 45 W at 220V no load 35 W at 220V Dimensions without heads 150 x 175 x 185mm W xD xH Humidity Up to 80 Weight 4 kg Altitude Up to 2000 m Pollution Pollution degree 2 Installation Category Conformity to Norms Refer to
20. er Tubing 30 L 30 awe E E 20 7 E 90 2 L 10 4 10 0 0 0 10 20 30 40 50 Pump Head Speed RPM 32 Set Up Speed Flow rate Chart Silicone Tubing 200 150 Flow mL min S o 50 HF Model Silicone Tubing 20 30 Pump Head Speed RPM 40 50 u4 pue uonoajas Buiqny 33 Set Up Recommendations 34 Recommendations e To ensure that consistent and accurate flow rates are maintained systemati cally replace peristaltic tubing as soon as it appears to be worn You can reduce wear on the tubing by running at slower speeds by using tubing of a larger diameter or by slackening the adjustment screws Conversely you should tighten the adjustment screws to reduce the level of pulsations which may also be reduced by applying some back pressure or by selecting a more rigid tubing material like PVC When the pump is not in use release the compression cams by pressing on the bevelled corners of the trigger key and slacken the tubing This increases the life of the tubing e Two small pieces of Isoversinic tubing are supplied in the standard accessory package Fit one piece to the pump head by pushing it onto the drain tube The purpose of the drain tube is to remove liquid from the pump head if the flow tubing breaks it limits the risk of liquid seeping into the speed control module which contains the motor and electronic circuitry Set Up e To prevent liqu
21. er to the following sections which describe how to use the keypad to control the pump or to Chapter 5 if the pump is to be remotely controlled 39 Operation Keypad Functions 40 EDAOSo s Keypad Functions The keypad has six keys plus a 3 digit and 2 symbol Liquid Crystal Display unit The keypad functions are summarized below Function Description Forwards Start the pump clockwise Backwards Start the pump counter clockwise Slower Decrease the pump speed Faster Increase the pump speed Stop Stop the pump Rabbit Set speed to 48 rpm Operation 4 Initialization 1 Install tubing as described in Chapter 3 uonezjemul 2 Switch the pump ON using the rear panel switch The speed is set at 12 5 rpm at delivery The last speed set before switching off is shown in rom on the keypad display 3 Select the desired speed by pressing the key to decrease the displayed value the key to increase the displayed value Pressing either key once changes the right hand digit by one pressing the key continuously makes the speed vary at an increasing rate 4 To start the pump press the 9 or the G key depending on the de sired direction of rotation A sign or a sign is then displayed to the left of the speed value Press the RABBIT key to purge or to prime the tubing The RABBIT key cannot be used until either the or the G key has been pressed The direction of pumping can be changed
22. g inside the instrument All other tubing is fitted to the pump head Gilson supplies two types of tubing peristaltic tubing with two retaining stops connection tubing without retaining stops In normal operations short lengths of peristaltic tubing are placed in the pump head With MP pump heads it is essential that peristaltic tubing with two retaining stops is used The calibrated stops ensure that the tubing is correctly tensioned when fitted to the head HF models can use either type of tubing The user then links the Minipuls 3 with other hydraulic components of the system for example chromatographic columns using connection tubing connectors and couplers of appropriate diameter and material Set Up Recommended Tubing by Flow Rate Type MP Models HF Models Flow Connecting Flow Connecting mL min mm id mL min mm id PVC up to 26 0 25 to 3 2 up to 236 2to8 Fluoroelastomer up to 38 0 5to 4 2to6 Silicone upto 45 0 6to2 8 up to 200 2to7 Polypropylene up to 14 0 5 to 2 up to 171 2 to 6 4 Type of Material Tubing made of various materials and with different internal diameters is available from Gilson The tubing sizes and materials recommended for MP and HF pump heads are shown in the above table Tubing sizes are specified as internal diameter id in millimeters mm Maximum flow rates are specified in milliliters minute For any given type of pump head the type of solution pumped determines
23. hapter 5 for more details relating to GSIOC connections and commands 8911199 3 21 Set Up Changing the Pump Head 22 Changing the Pump Head Follow these procedures to replace a pump head For the first installation only steps 2 to 6 are relevant The head can be replaced with any of the models listed in the Appendix 1 Unscrew the two mounting screws 2 Lift off the pump head 3 Place the two screws on the new pump head Set Up 4 Install the pump head on top of the speed control module in the desired position four are possible Do not use the position in which the drain tube is at the front 5 Turn the roller barrel so that the slot at the bottom of the barrel matches the black tenon of the speed control module Turning the barrel gently back and forth can help the user to engage it with the tenon When these two parts are properly en gaged the base of the pump head rests on top of the speed control module 6 Slide the pump head so that the two screws fall into their inserts and then tighten the two screws do not over tighten pesy dwnd 34 Buibueyo Set Up Tubing Selection and Fitting 24 Tubing Selection and Fitting Introduction There is a small Isoversinic drain tube inside the Minipuls 3 with an outlet at the base of the pump The tube which limits the risks resulting from a rupture of the peristaltic tubing is connected to the motor assembly to prevent liquid from seepin
24. he following connections between the FC203 204 and the Minipuls 3 The FC203 204 output 2 is program mable For proper operation with a Minipuls it must be set on OUT 2 on during run see the FC 203 204 U ser s Guide FC203 connector Minipuls 3 connector OUT 2 Pin 9 lt Pin 4 Ground OUT 2 Pin 10 gt Pin 3 STOP RUN Using an FC206 to Control a Minipuls 3 Make the following connections between the 206 and the Minipuls 3 TheFC206 output 3 is programmable For proper operation with a Minipuls it must be set on OUT 30n during run see the 206 U ser s Guide FC206 connector Minipuls 3 connector OUT 3 Pin 12 Pin 4 Ground OUT 3 Pin 11 gt Pin 3 STOP RUN Remote Control Control by a Gilson Sampler XL Samplers 22X 23X XL Samplers control a Minipuls 3 pump through GSIOC Liquid handling tasks provided by Gilson software are designed to control either a 401 402 Syringe Pump or a Minipuls 3 Peristaltic Pump Version A Samplers The connection between Minipuls 3 pumps and Gilson 22X 23X samplers is simple for a basic STOP RUN and CW CCW operation It requires the barrier strip connector for the sampler 25 pin SUB D connector three pieces of insulated wire and the green barrier strip connector supplied with the pump Basic Control Makethefollowing connections between the sampler and the Minipuls 3 19 duies uosji5 e Aq j01 U09 22X 23X Minipuls 3 Barrier Strip Barrier Strip Con
25. ical work If incidental spillage occurs carefully clean up the spillage taking into account the nature of the spilled liquid including all required safety measures This instrument must not be sterilized using an autoclave or any other mechanical device When you need to clean this instrument use one of the three following methods 1 A clean dry cloth 2 A cloth dampened with water 3 A doth dampened with soapy water Introduction If acloth dampened with soapy water is used to clean the instrument only domestic soap may be used No other form of detergent or chemical may be used When operating the Minipuls you must ensure that the fan works and that the ventilation slots are not obstructed in any way The following symbols appear on the instrument Symbol Explanation Alternating current D PROTECTIVE CONDUCTOR TERMINAL On Supply switch O Off Supply switch AN Caution risk of electric shock N Caution refer to User s Guide Symbols used on the rear panel are explained in Chapter 5 Thekeypad symbols are explained in Chapter 4 This instrument must not be directl y connected to humans for any purpose A Ayajes Introduction Description 2 Gilson s Minipuls 3 is a peristaltic pump designed for transfer ring fluids with a high level of speed stability and alow pulsation level It has many applications including the following e transferring solutions emulsions suspensions and gase
26. icrosoft C languages For other environments A software driver must be written to implement the Gilson protocol according to theGSIOC Technical M anual Thereare two basic command types the controller sends to a slave device buffered commands and immediate commands Remote Control 5 Slave commands execute buffered commands as background processes Immediate commands have a higher priority the slave interrupts the execu tion of a buffered command to execute an immediate command The Minipuls 3 command buffer is 32 characters long Connector Pin out The GSIOC Connector is a 9 pin male DOISD 0 1409 13 Nndwo30191 SUB D socket to a1 reserved which a controller a6 reserved micro computer 2 data from slave P a 7 data from slave or Gilson sampler 3 data from master can be connected a 8 data from master a 4 clock from master The pi n out is 9 clock from master a5 reserved opposite Control Modes The Minipuls 3 offers two levels of digital control mode keypad and remote The keypad mode is the default The Minipuls 3 normally works through the keypad and responds to immediate commands through GSIOC Itis similar to the local mode of some computer controlled devices In the remote mode the keypad is locked and the unit receives commands and key codes from the GSIOC Contact inputs are disabled 57 Remote Control Microcomputer Control GSIOC 58 Command Type lt nNnDDARX
27. id from leaking in the event of a tube breaking install the pump at a higher level than the tank and collector This arrangement eliminates gravitational flow e If the pump is used as part of a system where a back pressure is present for example against a chromatographic column the adjustment screw must betightened by one or two extra turns to enable the liquid to flow Where back pressure is present the maximum flow rates are less than the values that can be measured from the charts which are plots of values measured at atmospheric pressure refer to pages 31 to 33 e Usethe RABBIT key to prime the tubing fill it with liquid see Chapter 4 suonepuawwos y 35 Set Up Gilson Connectors and Couplers 36 Gilson Connectors and Couplers With reference to the Appendix Addi tional Accessories Gilson can supply connectors see table below for linking peristaltic tubing with connection tubing and couplers for other equip ment such as low pressure liquid chromatography columns Connectors Tubings are connected by simply pushing the tubings onto either end of a connector Plastic connectors are used for the larger diameter tubings Three types are available F1179941 small bore 1 to 2 mm to small bore F1179931 small bore 1 to 2 mm to large bore 2 to 3mm F1179951 largebore 2to 3mm to large bore Tubing ID 0 25 0 3 0 5 0 6 0 8 1 2 2 3 0 25 Sleeve 0 6 od
28. ing F1179931 F1179941 F1179951 Set of 10 PVDF connectors for 1 2 mm id tubing to 2 3 mm Set of 10 PVDF connectors for 1 2 mm id tubing to 1 2 mm Set of 10 PVDF connectors for 2 3 mm id tubing to 2 3 mm Connector Kits for Flanged Tubing F1825054 F1825056 F1825057 495051 Couplers 495036 F1410050 Set of 5 screw 8 cone for 1 0 1 65 mm id tubing 3 mm max od Set of 5 screw amp cone for 2 0 2 3 mm id tubing 4 mm max od Set of 5 screw amp cone for 2 8 3 2 mm id tubing 5 mm max od One connector 1 4 28 TPI to connect standard tube end fittings to flow tubes 1 to 3 mm id One PTFE coupler to connect high presure tube end fittings between 1 4 28 TPI and 10 mm standard thread Set of 5 PVDF couplers for linking two standard Gilson connection screws 1 4 28 TPI Appendices Polyvinylchloride PVC Tubing For use with medium concentration aqueous solutions acidic or basic Polyvinylchloride PVC Calibrated Peristaltic tubing 0 4 meter lengths of PVC peristaltic tubing with stops F117932 Set of 12 PVC Flow tubes of 0 25 mm id F117933 Set of 12 PVC Flow tubes of 0 38 mm id F117934 Set of 12 PVC Flow tubes of 0 51 mm id F117936 Set of 12 PVC Flow tubes of 0 76 mm id F117938 Set of 12 PVC Flow tubes of 1 02 mm id F117940 Set of 12 PVC Flow tubes of 1 30 mm id F117942 Set of 12 PVC Flow tubes of 1 52 mm id F117943 Set of 12 PVC Flow tubes of 1 65 mm id F117945 Set of 12 PVC Flow tubes of 2
29. kwards Start the pump counter clockwise Slower Decrease the pump speed Faster Increase the pump speed Stop Stop the pump 4 Rabbit Set speed to 48 rpm 15 Description 16 Set Up 3 This chapter contains 1 Key information relating to voltage selection and fitting the fuses You are strongly advised to read this information carefully 2 Detailed information relating to fitting the tubing flowrate selection and which connectors or couplings should be used 17 Set Up Electrical 18 Electrical Voltage Selection and Fitting Fuses The power receptacle includes an ON OFF switch and a fuse holder voltage selector The voltage is selected by removing the fuse holder inserting two fuses of the correct value into the fuse holder and then inserting the fuse holder into the power receptacle so that the small white arrow corresponding to the required voltage points to the align ment mark on the power receptacle body For safety reasons the speed control module is shipped without the fuses having been installed You must install two fuses of the type specified below and set the operating voltage that corresponds to the available power supply Two packs of slow blow type T fuses are supplied for operating at 100 V to 120 V fit two 500 mA fuses ref 6730054006 or for operating at 220 V to 240 V fit two 250 mA fuses ref 67300240062 Before connecting the power cord to the unit install two fu
30. l for further details The master device is usually a micro computer Gilson has developed hard ware and software interfaces for IBM PC or compatible computers The minimum IBM PC hardware configuration for the master device is as follows Hardware Requirements For computers using M S D O S W indows 9X orNT The CPU must have at least 512 KB of RAM The PC must have a keyboard a video monitor with ts controller card 9 cm 3 5 inch floppy disk drive with its controller an RS232C card and a 901SD 1011409 13 nNdwo30191W 55 Remote Control Microcomputer Control GSIOC 56 Gilson 605 RS232 Adapter or 506B C System Interface For other environments Other computers require an RS232C port and a Gilson 506B C System Interface or 605 RS232 Adapter The RS232C port must be able to be formatted for eight data bits and one even parity bit Refer to the 605 RS232 Adapter User s Guide for a full description of the adapter operational details and information relating to the GSIOC protocol Refer to the 506 System Interface User s Guide for information relating to the use of the interface Software Requirements For computers using MS DO S Windows 9X or NT The software environment must include PC DOS or MS DOS V3 0 or above a programming language and a Gilson 706 Device Driver software The user may develop application programs in BasicA or GW Basic Borland Turbo Pascal Microsoft Pascal or M
31. nector a OT __ T i G sin Pin AUXIL 0 S Pin 3 STOPIRUN uat of VO es et minute 22X 23X Command Pint Pin14 Action ASPIR 9 t ON ON Run pump CW for time t DISP 9 t ON OFF Run pump CCW for time t PAUSE OFF X Stop the pump 53 Remote Control Control by a Gilson Sampler 54 Full Control 22X 23X samplers cannot control Minipuls 3 pumps through GSIOC However the pump is equipped with a specific feature that allows the use of contact inputs to transmit speed data 1 Set the pump ID number to 99 2 Connect the sampler to the pump as follows 22X 23X Minipuls 3 Barrier Strip Barrier Strip Connector Pin 8 Ground Pin 1 AUXIL 0 Pin 14 AUXIL 6 Pin 17 AUXIL 4 Pin 6 Ground Pin 3 Clock Pin 1 Data Pin 5 Start stop transmission List Remote Control Microcomputer Control GSIOC Introduction to GSIOC The Gilson Serial Input Output Channel GSIOC is a bidirectional bus that connects a master device usually called controller or system controller with as many as ten slave devices with RS422A specifications or thirty two with RS485 specifications The master communicates with one slave at a time The slave is identified by a unique identification number in the range 0 63 When the master identifies a slave it connects on the channel as the previous slave disconnects The GSIOC bus implements RS422A RS485 transmitters and receivers Refer to the GSIOC Technical Manua
32. ode Keypad and Remote Function Master reset Response format is echoed Command l Type Immediate Mode Keypad and Remote Function Request Contact Input Status Response format Where Default response ap a reflects pin 3 START STOP input b reflects pin 1 CW CCW input a and b take values of either O or 1 1 is an inactive or high input open 0 is an active or low input closed w Command K Type Buffered Mode Remote Function Input remote keystrokes Mimic key actions Syntax K codes Parameters String of characters as follows lt backwards CCW gt forwards CW faster slower H stop amp rabbit Comments All other characters are ignored A buffered K command must be the last in a command string To makethis command work properly the Minipuls should not be disconnected from the system master until the buffer is empty To ensurethat this is truethe master should send another command such as an empty string immediately after any string containing a K command Completion of thefirst string is implied as soon as the second is accepted 59 Remote Control Microcomputer Control GSIOC 60 Command Type Mode Function Response format Where Default response Command Type Mode Function Syntax Parameter Comment Command Type Mode Function Response format Where Default response K
33. ontrol Mode Switches between modes Sm m K for keypad or R for remote mode Vv Immediate Keypad and Remote Request Analog Input Status vvv vvv is a three character code range 000 255 255 255 corresponds to 5 V or open circuit Command Comment To set the speed at 25 rpm 0 Master Reset B SR Set remote mode This sets the speed B R2500 Set speed to 25 00 rpm without starting the pump it does not rotate alii dc key Command Comment entry sequence I Master Reset B SR Set remote mode CCW FULL SPEED B K lt Select CCW direction B K amp Full speed rabbit Then B toallow K lt and K amp to work I Master Reset B SRK lt amp _ Linked buffered commands empty string QOISD 1011409 13 ndwo30191W 61 Remote Control 62 Appendices 6 Thischapter contains part numbers and technical data specifications 63 Appendices 6 Part Numbers and Accessories Part Numbers and Accessories Six pump heads are available four standard models 1 2 4 or 8 channels and two HF models 2 or 4 channels The speed control module which may be set to operate at either 220 240 V or 100 120 V refer to Chapter 3 is supplied with an accessory package containing the fuses and power cords for both operating ranges Part Numbers Pump Head R4 Model R1 R2 R8 R2 HF R4 HF F117604 F117800 F117606 F117608 F117830 F117
34. rate the pump Chapter 5 contains information concerning remote control in cluding how to make the electrical connections to other instru ments and GSIOC instructions Chapter 6 is an appendix containing details of the accessories and the technical data Introduction Unpacking Unpacking Each complete Minipuls 3 is shipped in two boxes Box 1 contains the speed control module and the standard accessory package see the A ppendix Box 2 contains the pump head from the six available models seethe A ppendix Additional speed control modules induding the standard accessory pack age Box 1 or additional pump heads Box 2 may be ordered separately Upon receipt of your Minipuls 3 un pack it and check that all of the parts are included even if it is not for imme diate use Keep the packing because in the event that the unit is returned to the factory the original packing must be used to return equipment under warranty Report any loss or damage immediately Introduction Safety Read this section carefully before installing and operating the equipment The instrument described in this User s Guide is alow pressure pump that should only be used in the labora tory or similar indoor environment by qualified personnel If the instrument is used in amanner not specified by Gilson the protection provided by the instrument may be impaired For safe and correct use of the instru ment it is essential that bo
35. s at up to 200 C e propelling liquids through chromatographic systems against a back pressure of up to 500 kPa for example when controlled by a Gilson fraction collector e automation of biological analyses by proportionally mixing a sample with several reagents flow injection analysis e continuously sampling the components of a production process reactors fermenters and so on e formation of gradients concentration pH and so on The Gilson Minipuls 3 is powered by a hybrid stepper motor and controlled by an integral microprocessor by using the front panel keypad see Chapter 4 It can also be remotely controlled from a master device such as an IBM PC or by electrical contacts see Chapter 5 Description Introduction 10 Introduction The main components are as follows e The speed control module contain ing a die cast sub chassis onto which is mounted the stepper motor and its controller board microprocessor e An interchangeable pump head e A back panel assembly consisting of an L shaped plate onto which are mounted the power supply assembly the cooling fan and control sockets e A keypad and LCD unit Description Speed Control Module The figure shows a general view of a Minipuls 3 before the head is installed Keypad Controller 3 NPO N 01JUO0OH paeds 11 Description Pump Head 12 Locking E Drain tube Compression cam Pump Head Pump hea
36. ses in the voltage selector and set the voltage as follows 1 Pull the voltage selector out of the power receptacle located on the lower left hand corner of the back panel Set Up 3 2 Pull out the drawer as shown in the figure before inserting the fuse into x the clips 2 3 Push the drawer and the fuse into S position 4 Perform the same operation for the second fuse on the other side 5 Reinsert the voltage selector so that the arrow ON OFF switch next to the required operating voltage points downwards to the small white rectangle on the power receptacle When correctly installed the text indicating the required voltage range will bethe right way up The voltage is factory set to 220V refer to the figure Voltage selector Remote Control Connections Electrical contacts The Minipuls 3 can be started and stopped remotely using electrical inputs The direction of rotation and pump speed may also be changed The electri cal contacts are made using the barrier strip connector on the rear panel The contacts which are numbered 1 through 6 have the functions listed on the next page Contacts 2 4 and 6 are ground connections 19 Set Up Electrical 20 e Pair 1 2 is a contact input that changes the pumping direction e Pair 3 4is a contact input that starts stops the pump e Pair 5 6controls the speed of the pump The connections are made using insu lated wire and barrier strip connectors
37. th operating and service personne follow generally accepted safety procedures as well as the safety instructions given in this User s Guide Cleaning installation dismantling maintenance adjustment and repair should only be performed by personnel trained in such work and who are aware of the possible dangers involved Voltages present inside the instrument are potentially dangerous If thereisa problem with the instrument the power cable should be removed until qualified service personne have repaired it This is to prevent anyone from inad vertently using the instrument thus causing possible harm to themselves or damage to the instrument itself Ayajes Introduction Safety The leakage current of the instrument is within the limits allowed by safety standards of laboratory equipment An efficient ground connection is imperative for the physical protection of the user Power supply cord reference 7080316106 is for usein France and Germany Power supply cord reference 7080316105 is for use in USA and Canada For other countries contact your local Gilson representative You must only use the type of fuse described and specified in this document 0 5 Amp type T slow blow is for use with power supplies between 100V and 120V 0 25 A mp type T slow blow is for use with power supplies between 220V and 240V Adequate protection such as ventilation must be provided if dangerous liquids are used in the analyt
38. the type of peristaltic tubing to be used refer to the table on page 27 More rigid materials such as PVC produce smaller pulsations For exam ple when pumping water at 45 rom pressure 100 kPa typical values for pressure pulsations are 5 with 6 mm id PVC tubing and 20 with 6 mm id silicone tubing Pu pue uolsajas Buiqn 25 Set Up Tubing Selection and Fitting 26 Diameter and Flow Rate Select the peristaltic tubing diameter that gives the desired flow rate by reference to the Speed Flow Rate Charts page 31 to page 33 These graphs represent the values obtained in laboratory tests where the liquid was allowed to flow freely from the tubing against atmospheric pressure only There was no additional back pressure e g a chromatographic column The selection of peristaltic tubing isa compromise between minimizing the pulsations and maximizing the life time of the tubing When making this choice the following points should be considered the smaller the diameter the smaller the pulsations and the faster the head has to rotate leading to a reduction of peristaltic tubing lifetime the bigger the diameter the bigger the pulsations and the slower the head has to rotate leading to increased peristaltic tubing lifetime That is the pulsations increase when the flow rate decreases and when the internal diameter of the tubing increases Set Up Tubing Selection and Fitting Recommen
39. tion and if neces sary replace the fuses If the display remains blank the transformer or the fan assembly could be faulty Contact the service agency If the fan runs Check the electronics by pressing the CW or CCW key If the pump head does not rotate there could be a problem with the electronics faulty board Contact the service agency Dead Keypad If the and the keys have some effect the pump is probably under remote control If not the keypad could need replacing Contact the service agency No GSIOC Response Check the GSIOC ID number GSIOC cables connectors and GSIOC interface module If these actions fail to correct the problem there could be a problem with the electronics faulty board Contact the service agency Worn or Faulty Pump Head Replace the pump head Remote Control 5 Remote contact control is only available after the stop key of the Minipuls 3 is pressed Remote contact control is not available if the Minipuls 3 is already under GSIOC control 47 Remote Control Electrical Contact Control 48 Pump Speed ANALOG Electrical Contact Control There are two contact inputs and one analog input available on the 6 pin barrier strip connector These TTL low level active inputs 0 to 5 Volts have athreshold of 2 Volts and are pulled up to 5 Volts with a high impedance They are protected against AC voltages up to 264 Volts rms for an unlimited period of time
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