VIAFLO ASSIST Operating instructions
Contents
1. 300 0 ul 8 channel pipette 50 0 1250 ul 4624 4631 12 channel pipette 0 50 12 50 ul 12 channel pipette 5 0 125 0 ul 4632 12 channel pipette 10 0 300 0 ul 12 channel pipette 50 0 1250 ul 4633 4634 4641 16 channel pipette 0 50 12 50 ul 16 channel pipette 5 0 125 0 ul 26 4642 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Accessories T 3 Consumables Reagent Reservoirs Part No 10 ml Disposable reagent reservoirs individually sealed 4331 30 reservoirs per case sterile Disposable reagent reservoirs four sleeves of 50 reservoirs 4332 per case sterile Reservoir Base 10 pack 4306 25 ml Disposable reagent reservoirs individually sealed 4311 30 reservoirs per case sterile Disposable reagent reservoirs four sleeves of 50 reservoirs 4312 per case sterile Reservoir Base 10 pack 4304 100 ml Disposable reagent reservoirs individually sealed 4321 30 reservoirs per case sterile Disposable reagent reservoirs four sleeves of 50 reservoirs 4322 per case sterile Reservoir Base 10 pack 4305 www integra biosciences com 27 VIAFLO ASSIST Operating instructions VOO Accessories GripTips for all VIAFLO Electronic Pipettes Part No 12 5 pl Bulk pack 1 bag of 1000 tips non sterile LONG 4401 LONG 5 inserts of 384 tips non sterile LONG GREEN CHOICE 4402 5 boxes of 384 tips non sterile LONG 4403 5 boxes of 384 tips sterile LONG 4404 5 boxes of 384 tips2. Disp Speed Set speed of all dispensing steps 1 low 10 fast Height Set the height for the dispensing steps TipTouch It is highly recommended to activate a tip touch after a dispensing step It removes drops that may cling to the pipette tips Select where the tip touch should be performed If set to Liquid the tips will dip into the center of the wells If set to Side the tips will touch to the side of the wells Define the height for the tip touch Las The Last Dispense is discarded back to the source container A Last Dispense Dispense volume should be selected to improve accuracy and precision Suggested volume at least 5 of the pipette s maximum volume Press OK to toggle between ON green tick default and OFF red cross f ON define the volume of Last Dispense and the blowout height Set the Blowout height below the liquid in the wells for best results Press gt to save your settings This will return you to the list of Repeat Dispense programs www integra biosciences com 19 VIAFLO ASSIST Operating instructions VOO Operation 4 2 5 Variable dispense mode Parameters The settings remain the same as already described in section 4 2 4 Repeat dispense mode on page 18 Only one dispense option is unique to variable dispense and not described in the iy t section Option Description Set the different dispense volumes for every repeat dispense step 1ton Press to save y
3. sterile filter LONG 4405 5 inserts of 384 tips pre sterilized LONG GREEN CHOICE 4406 12 5 ul Bulk pack 1 bag of 1000 tips non sterile 4411 5 inserts of 384 tips non sterile GREEN CHOICE 4412 5 boxes of 384 tips non sterile 4413 5 boxes of 384 tips sterile 4414 5 boxes of 384 tips sterile filter 4415 5 inserts of 384 tips pre sterilized GREEN CHOICE 4416 125 ul Bulk pack 1 bag of 1000 tips non sterile 4421 5 inserts of 384 tips non sterile GREEN CHOICE 4422 5 boxes of 384 tips non sterile 4423 5 boxes of 384 tips sterile 4424 5 boxes of 384 tips sterile filter 4425 5 inserts of 384 tips pre sterilized GREEN CHOICE 4426 300 ul Bulk pack 1 bag of 1000 tips non sterile 4431 5 inserts of 96 tips non sterile GREEN CHOICE 4432 5 boxes of 96 tips non sterile 4433 5 boxes of 96 tips sterile 4434 5 boxes of 96 tips sterile filter 4435 5 inserts of 96 tips pre sterilized GREEN CHOICE 4436 1250 wl Bulk pack 1 bag of 500 tips non sterile 4441 5 inserts of 96 tips non sterile GREEN CHOICE 4442 5 boxes of 96 Tips non sterile 4443 5 boxes of 96 Tips sterile 4444 5 boxes of 96 Tips sterile filter 4445 5 inserts of 96 tips pre sterilized GREEN CHOICE 4446 28 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions V00 Imprint 2013 INTEGRA Biosciences AG All rights to this documentation are reserved In particular the rights of reproduction processing translation and the form of presentation lie wi
4. Pipette 15 Display 16 Back button to navigate backward 17 Touch wheel spin to scroll and move the cursor 18 OK button to make a selection 19 Left and right arrow buttons for selections 20 Purge button to empty tips 21 Run key to start operations 22 Tip ejector 23 Finger hook facilitates easy operation 24 Volume indicator label color matches GripTip box insert 24 2 3 2 Display The Display shows all pipetting options Battery life indicator ASSIST Pipet INTEGRA Repeal Dispense BE Repeat Dispense Sample Dilute Pipet Mix i Manual Fipet OK Reverse Pipet Serial Dilution select program Current mode Seria Dilution mam Press RUN to go Instruction Active step Move Column 1 Volume ss Help __ Options 8 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Installation 3 Installation 3 1 Operating environment VIAFLO ASSIST has been designed for use in a laboratory It shall be operated in a dry and dust free location with a temperature of 5 40 C and a maximal non condensing relative humidity of 80 96 see 6 1 Environmental conditions on page 25 3 2 Turn on off the VIAFLO ASSIST device Connect VIAFLO ASSIST to the power supply with the supplied mains adapter Turn on VIAFLO ASSIST by pressing the On Off key 14 which is confirmed by the green LED lighting up To turn off VIAFLO ASSIST press the On Off key two seco
5. reference is the Plate sledge 2 e Adjust the tip height If the sample source container is located on the plate sledge use the Left and gt Right arrow keys to move the sample aspiration spot underneath the GripTips If the sample is in the reservoir use the Right arrow key to move the plate sledge out of the way Press OK to display the setting dial Press the A Up and V Down keys to position the GripTips at the correct aspiration height and press OK Alternatively use the Touch wheel to enter the aspiration height directly if known and press OK Set the sample volume that will be transferred from well to well Asp Speed Set speed uniquely for aspiration 1 low 10 fast Mixing Mixes the sample in the source container before the first aspiration Press OK to toggle between ON green tick and OFF red X default f ON define Mix volume Mix Speed and number of Mix Cycles Dispense Mix options Option Description 0000000000 First Column Set the destination for the first transfer of the serial dilution column 1 24 or First Row or row A P Rows or Set the number of rows or columns to dilute 1 n including the first row Columns column 16 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Operation This is the dispense height in the target container It defines the dispense mix and aspiration height e Use the Left and Right arrow keys to move the pla
6. INTEGRA VIAFLO ASSIST Operating instructions 127950 V00 INTEGRA Declaration of conformity Konformit tserkl rung C C D claration de conformit Declaraci n de conformidad Dichiarazione di conformit INTEGRA Biosciences AG 7205 Zizers Switzerland declares on its own responsibility that the product erklart in alleiniger Verantwortung dass das Produkt d clare sous sa responsabilit exclusive que le produit declara bajo su propia responsabilidad que el producto dichiara sotto la propria responsabilit che il prodotto VIAFLO ASSIST Model 4500 in accordance with EC directives gemass der EU Richtlinien est conforme au terme directives CE de acuerdo con las directivas CE in conformit alle direttive CE 2006 95 EC Low voltage equipment 2004 108 EC Electromagnetic compatibility 2011 65 EC Restriction of Hazardous Substances 2002 96 EC Waste Electrical and Electronic Equipment is in compliance with the following normative documents mit den folgenden normativen Dokumenten bereinstimmt aux documents normatifs ci apr s cumple las documentos normativos soddisfa le normative seguenti EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61326 1 Electrical equipment for measurement control and laboratory use EMC requirements Standards for Canada and USA CAN CSA C22 2 Safety requirements for electrical
7. ciences AG VIAFLO ASSIST Operating instructions VOO Installation 3 6 2 Setting tip type VIAFLO II Electronic Pipettes of 12 5 ul size can be used with standard or long GripTips In order to teach the correct heights for VIAFLO ASSIST movements the used tip type must be specified Go to the Toolbox of the VIAFLO pipette select ASSIST press OK Tip Type Select Tip Type and press OK Standard Use the Touch wheel to highlight Standard or Long Press OK to select the correct tip type green tick and Save gt your settings 3 6 3 Connection between VIAFLO ASSIST and pipette The first time a specific VIAFLO pipette is going to be connected to the VIAFLO ASSIST both instruments need to be paired Scroll to the Toolbox and press OK From the ASSIST option select Bluetooth Pairing Switch OFF and ON the VIAFLO ASSIST device and wait approx 30 Switch OFF and ON sec until the message Pairing successful is displayed Press OK ASSIST and wait approx 30 sec m Select the ASSIST mode in the Main Menu of the VIAFLO pipette and Repeat Dispense press OK The pipette establishes the Bluetooth connection Variable Dispense Custom When the blue Bluetooth symbol next to the battery indicator is displayed and the Bluetooth control LED 13 alights both instruments are connected If the connection fails press lt to retry the connection or refer to 4 4 Troubleshooting on page 23 Alternatively continu
8. d or yellowing However this will not affect the performance of the device in any way 6 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Description of the device 2 Description of the device 2 1 Scope of delivery e VIAFLO ASSIST VIAFLO II Electronic Pipette to be ordered separately Mains adapter Sample pack 100 ml reagent reservoir CAUTION Verify the scope of delivery when unpacking the device and check for potential transportation damage Do not operate a device that is damaged instead contact your local dealer 2 2 Overview of the VIAFLO ASSIST 2 2 1 VIAFLO ASSIST device 1 Tower 2 Plate sledge 3 Power connection 4 Keypad 5 Reservoir position 6 Instrument base 7 Pipette holder on sliding arm 8 Gripper lever to release the pipette 9 VIAFLO Electronic Pipette 2 2 2 Keypad 24 E ang the zaxis to teach pipeting heights 11 4 Left and gt Right arrow keys move the plate 10 11 12 13 14 sledge along the X axis to adjust the plate sledge position 12 Start Pause key to start stop operations Green LED blinks press to start operation homing Green LED lights operation performed 13 Bluetooth control LED lights blue if connection is active and Error LED flashes red in case of error 14 On Off key lights green if On www integra biosciences com T VIAFLO ASSIST Operating instructions VOO Description of the device 2 3 Overview of VIAFLO II Electronic Pipette 2 3 1
9. e Offline lt During offline mode VIAFLO ASSIST cannot be operated It is possible to create programs however without teaching of active heights Press the Start Pause key when it is blinking to home the VIAFLO ASSIST device WARNING Remove hands from VIAFLO ASSIST during homing After homing the LED of the Start Pause key will turn off www integra biosciences com 11 VIAFLO ASSIST Operating instructions VOO Installation 3 6 4 Adjusting the plate alignment The Plate Alignment option is used to align the pipette s tips along the X axis with the center of the wells in the first row column of a microplate depending on plate orientation Select ASSIST on the Toolbox and press OK Plate Alignment Select Plate Alignment and press OK Tip Type i Bluetooth Pairing Orientation landscape Define the number of wells of your plate e g 96 for a 96 well plate and choose the plate orientation from Place pipette on ASSIST move to Row A Co 1 either landscape or portrait Use the Touch wheel to and press Set select an option and press OK With GripTips attached install the VIAFLO pipette on the VIAFLO ASSIST and put a plate on the plate sledge Use the Left arrow key to positon row A column 1 below the tips Press the arrow keys until the tips target the center of the wells Press Set to save this position NOTE Position alignments and pipetting with 384 well plates require a 16 channel VIAFLO II Electr
10. equipment for measurement No 61010 1 control and laboratory use General requirements UL Std No 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements FCC Part 15 ClassA Emission Zizers September 9 2013 8 huide FI Elmar Morscher Thomas Neher CEO Quality Manager VIAFLO ASSIST Operating instructions V00 Table of Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Introduction MEME MIDOIS Used 5 1 2 intended us s sleale 5 Lo SALLY NOLES Loses diae sen 6 Description of the device 2 1 SCOPE Or dell VOD aie iti adelaide T 2 2 Overview of the VIAFLO ASSIST i 7 2 2 1 VIAFLO ASSIST device sacre raies 7 222 Corieoio m 7 2 3 Overview of VIAFLO II Electronic Pipette 8 SRM eic LT 8 2 9 2 DSpace A ED ne 8 Installation 3 1 Operating environment ss 9 3 2 Turn on off the VIAFLO ASSIST device 9 3 3 Turn on off the VIAFLO II Electronic Pipette 9 3 4 Adapting the pipette holder ii 9 3 5 Attaching and removing a VIAFLO II Electronic Pipette 10 S10 LOOO SENINGS t RS 10 3 6 1 Enabling ASSIST mode seen 10 36 2 Setting tip lV Pe xcci to retire ele 11 3 6 3 Connection between VIAFLO ASSIST and pipette 11 3 6 4 Adju
11. er the coordinates manually on the pipette s screen To show the actual coordinates tap any of the Arrow keys on the VIAFLO ASSIST device Click gt on the pipette to set the coordinates The plate sledge travels the set distance in X direction relative to the current position by X mm Setting a negative value mm moves it to the left setting a positive value mm moves it to the right The distance from well to well for a 96 well plate is 9 mm and for a 384 well plate 4 5 mm Moves the pipette in Z direction to the selected coordinates Use the Arrow keys on VIAFLO ASSIST to teach the position or dial the height manually on the pipette s screen Press OK on the pipette to accept the settings www integra biosciences com 21 VIAFLO ASSIST Operating instructions VOO Operation Performs a blow out A blow out needs to be performed after the last dispense to remove liquid that may cling to the tips Note When using Purge to empty the tips a blowout blowin is performed automatically and does not need to be programmed After a blow out a blow in has to follow at some point It does not have to follow immediately and can have steps in between E g after the blow out a move step can be programmed to move the tips out of the liquid and is then followed by the blow in A delay is a pause between the last and the next step Define a delay time in seconds or a manual input pressing the Run key to continue A loop re
12. ers like Rows Columns or Count Alternatively use the Touch wheel to enter the height directly if known distance between Plate sledge 2 and Tip end Press OK on the pipette to save current settings Clear Height The travel height at which the GripTips move from well to well The tips must be above the plate Press OK to display the setting dial showing the current selected height Use the Left and gt Right arrow keys of VIAFLO ASSIST to move the plate sledge under the pipette Press the A Up and W Down keys to position the GripTips to the desired height above the plate www integra biosciences com 15 VIAFLO ASSIST Operating instructions VOO Operation 4 2 3 Serial dilution mode Plate setup See section 4 2 2 General parameters of all pipetting modes on page 15 Sample options Option Descripton S Use the Touch wheel to choose one of the source containers where the initial sample is aspirated from e Reservoir reagent reservoir e Tube Strip for PCR tubes e Tip samples can be aspirated before the automatic program is started e Column 1 24 or Row A P the sample is in the selected column row of the plate This is the sample aspiration height of the source container If Reservoir or Tube Strip are chosen as source container the height is the distance between the Instrument base 6 and the end of the GripTip For Column or Row the lower point of
13. ight arrow keys to move the dispense spot underneath the GripTips If the last dispense is performed in the reservoir use the P Right arrow key to move the plate sledge out of the way While the setting dial is displayed press the 4 Up and V Down keys Press gt to save your settings This will return you to the list of Serial Dilution programs www integra biosciences com 17 VIAFLO ASSIST Operating instructions VOO Operation 4 2 4 Repeat dispense mode Plate setup See 4 2 2 General parameters of all pipetting modes on page 15 Aspirate Source options Option Descripton S Use the Touch wheel to choose one of the source containers where the initial sample is aspirated from e Reservoir reagent reservoir e Tube Strip for PCR tubes e Tip samples can be aspirated before the automatic program is started e Column 1 24 or Row A P the sample is in the selected column row of the plate Height Start The VIAFLO ASSIST will automatically refill the GripTips if the number of dispenses exceeds the maximum volume of the pipette The first aspiration will be at the start height and for consecutive aspiration steps the pipette will go lower until it reaches the end height If Reservoir or Tube Strip are chosen as source container the height is the distance between the Instrument base 6 and the end of the GripTip For Column or Row the lower point of reference is the Plate sledge 2 Set the height for the first a
14. integra biosciences com 5 VIAFLO ASSIST Operating instructions VOO Introduction 1 3 Safety notes VIAFLO ASSIST complies to the recognized safety regulations and is safe to operate VIAFLO ASSIST can only be operated when in intact condition and while observing these operating instructions The device may be associated with residual risks if it is used or operated improperly by untrained personnel Any person operating the VIAFLO ASSIST must have read and understood these operating instructions and particularly the safety notes or must have been instructed by supervisors so that safe operation of the device is guaranteed Regardless of the listed safety notes additional applicable regulations and guidelines of trade associations health authorities trade supervisory offices etc must be observed Do not open or modify the VIAFLO ASSIST in any way Repairs may only be performed by INTEGRA Biosciences AG or by an authorised after sales service member Parts may be replaced with original INTEGRA Biosciences parts only WARNING Do not use the VIAFLO ASSIST near flammable material or in explosive areas Also do not pipette highly flammable liquids such as acetone or ether When handling dangerous substances comply with the material safety data sheet MSDS and with all safety guidelines such as the use of protective clothing and safety goggles NOTE Prolonged exposure of the VIAFLO ASSIST to UV light can cause discolouration an
15. n pathogens VIAFLO ASSIST needs to be decontaminated before sending it to service and the declaration on the absence of health hazards must be signed This is necessary to protect service personnel 24 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions V00 Technical Data 6 Technical Data 6 1 Environmental conditions pp operation Temperature range operation 5 40 C Temperature range storage 10 50 C Humidity range Max rel humidity 80 for temperatures up to 31 C decreasing linearly to 50 rel humidity at 40 C 6 2 Specification of the device Power supply Input 100 240VAC 50 60Hz Output 24 VDC 3 0 A Dimensions H x D x W 400 mm x 360 mm x 340 mm with pipette attached height 510 mm Weight 10 kg www integra biosciences com 25 VIAFLO ASSIST Operating instructions VOO 7 Accessories 7 1 Accessories for VIAFLO Electronic Pipettes Charging options and Bluetooth Accessories Part No 4200 Universal voltage power supply Lithium ion battery 3 7 V 4205 4210 Single pipette charging stand 4211 VIALINK programming stand 4 pipette charging stand 4215 4221 VIAFLO II pipette Bluetooth module General O ring removal tool 7 2 Accessories for VIAFLO ASSIST VIAFLO II Electronic Pipettes Part No 130 00731 00 Part No 8 channel pipette 0 50 12 50 pl 8 channel pipette 5 0 125 0 ul 4621 4622 4623 8 channel pipette 10 0
16. nds It s LED switches off 3 3 Turn on off the VIAFLO II Electronic Pipette Press and release the Run key 21 to turn on the pipette To turn off the pipette press and hold the Back button 16 for 3 seconds 3 4 Adapting the pipette holder The pipette holder can be adapted to the type of the VIAFLO II Electronic Pipette To adapt the holder for an 8 channel pipette push the silver lever down as shown beside The width of the pipette holder is now reduced Lift the lever upwards if a 12 channel pipette should be inserted www integra biosciences com 9 VIAFLO ASSIST Operating instructions VOO Installation 3 5 Attaching and removing a VIAFLO Il Electronic Pipette Rotate the lower hosing of the VIAFLO II Electronic Pipette by 90 degrees as displayed in the picture To install a pipette hold it at an angle and insert it into the pipette Gm holder Then lift up until the black gripper snaps in place To release the VIAFLO II Electronic Pipette lift the black gripper by pushing down on one end 3 6 Toolbox settings 3 6 1 Enabling ASSIST mode The ASSIST mode is hidden from the Main Menu of the VIAFLO pipettes by default Use the Touch wheel to select Toolbox from the Main Menu and press OK Repeat Dispense Sample Dilute Select Preferences and then Main Menu Enable the ASSIST mode by Manual Pipe i pressing OK green tick and press gt to save your settings Reverse Pipet 10 INTEGRA Bios
17. o Toolbox gt ASSIST and select Bluetooth Pairing Follow the instructions on the pipette screen Follow the instructions on the pipette display Contact service for VIALINK special firmware update Perform plate alignment see 3 6 4 Adjusting the plate alignment on page 12 Enter the tip type used see 3 6 2 Setting tip type on page 11 23 VIAFLO ASSIST Operating instructions VOO Maintenance 5 Maintenance WARNING Always turn off power and disconnect the VIAFLO ASSIST from the mains when carrying out maintenance work 5 1 Cleaning The materials used on the exterior of the VIAFLO ASSIST support regular cleaning intervals Clean the external components with a lint free cloth lightly soaked with mild soap solution in distilled water or with a 70 dilution of Isopropyl or Ethanol Never use acetone or other solvents 5 2 Decontamination Decontamination is not required for the proper functioning of the VIAFLO ASSIST Only if any surfaces have been in direct contact with biohazardous material they must be decontaminated in accordance to good laboratory practice e g with the following disinfectants e Ethanol 70 96 Microcide SQ 1 64 Glutaraldehyde solution 4 Virkon solution 1 396 Follow the instructions provided with the reagents 5 3 Servicing For any service or repairs please contact your local service technician WARNING If working with infectious materials e g huma
18. onic Pipette 3 7 Charging the battery of the VIAFLO II Electronic Pipette The battery indicator in the upper right corner of the pipette screen informs about the battery status When it turns red the pipette needs to be charged CAUTION STOP Use only the approved INTEGRA battery power supply or charging stand Useofan incompatible power transformer can damage the pipette The battery can be charged using either the Universal Voltage Power Supply UPS or a charging stand see 7 1 Accessories for VIAFLO Electronic Pipettes on page 26 Insert the UPS connector into the receptacle on the top back of the pipette Plug the UPS into a wall outlet 12 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Operation 4 Operation 4 1 Running a program From the ASSIST menu select the Pipetting mode and the stored program you want to run and press OK Press the Run key 21 You are prompted to place the pipette on the VIAFLO ASSIST device see 3 7 Charging the battery of the VIAFLO Il Electronic Pipette on page 12 When attached press the green blinking Start Pause key 12 on the VIAFLO ASSIST device It switches to solid green and the program will be performed automatically WARNING Keep hands out of area of moving VIAFLO ASSIST parts during the run A program can be interrupted by pressing the Start Pause key 12 Either press the Start Pause key again to continue the program or pres
19. our settings This will return you to the list of Variable Dispense programs 20 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Operation 4 2 6 Custom program mode The custom program allows for a step based set up of individual pipetting protocols Each program can contain up to 98 steps From the ASSIST Menu select Custom press OK and define a name of your program Cueto The first line is highlighted Press OK No Programs Aspirate Use the Touch wheel to select a first step from the menu Press OK define the required parameters and press OK to add the step After adding the first step the selection should now be on the second line Press OK again to define the second step Continue adding steps until your entire pipetting protocol is defined The individual steps based upon the following basic operations Step Description Sets an aspiration volume and speed Sets a dispense volume and speed Mix Performs a mixing cycle Defines the number of mix cycles mixing volume and mix speed Purge Purges all remaining liquid currently in the GripTips with the selected purge speed Prompt Pauses the program and displays a message Three lines with a total of 30 characters are available To continue the program press the Run key Move X Z Moves the pipette in Z direction and the plate sledge in X direction to the selected coordinates Then use the Arrow keys on VIAFLO ASSIST to teach the position or ent
20. peats the steps between the selected step and the loop command as many times as defined E g if the program reaches the loop step it goes back to step 3 and repeats the steps until there 2 times The number of program steps can often be shortened by adding a loop Note Nested loops loops inside loops are not allowed When finished press gt to save the Custom program To run the program press OK 4 3 Modify existing programs At any program display use the Touch wheel to highlight an existing program Press lt Options use the Touch wheel to select an option View Edit Delete Copy Rename to modify the program and press OK Options 22 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO 4 4 Problem The connection between pipette and ASSIST cannot be established Error LED 14 blinks red After firmware update the Error LED 14 blinks red and VIAFLO ASSIST cannot be Started GripTips are not aligned in X posi tion The tip height is not correct using 12 5 ul Grip Tips Troubleshooting Probable cause The two instruments have not been paired or the pairing was lost VIAFLO ASSIST motor lost steps during movement Pipetting heights were set wrong and the pipette crashed into the plate No firmware installed Plate alignment not set Wrong tip type set www integra biosciences com Operation Remedy From the main menu of the pipette go t
21. programs can later be 0123456789 f i bag renamed see 4 3 Modify existing programs on page 22 Define all parameters of your program and press Save D Clear Height 17 0 mm Sample Location Column 1 To run the program select the stored program and press OK on the VIAFLO Electronic Pipette see 4 1 Running a program on page 13 14 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Operation 4 2 2 General parameters of all pipetting modes NOTE Perform all position settings with GripTips attached iid 1e 5 ul volume Mp ie the correct tip type needs to be defined first see 3 6 2 Setting tip type on page 11 Plate setup The following basic plate setup is part of every pipetting mode Use the Touch wheel to select an option define the required parameters and press OK If a parameter is out of range the VIAFLO pipette beeps Press Error gt to read the error message NOTE Keep the Arrow keys 4 gt A V pressed to increase the movement speed of the plate sledge or pipette holder during position teaching Option Description oo Wells Press OK and use the Touch wheel to choose the kind of well plate used 6 384 Press OK Orientation Choose the orientation of the plate Press OK to toggle between landscape and portrait 19000000 1200990000 Xx int 19909 LA iss egcccccccecs EH t3 Landscape Portrait Columns Rows Note Switching to portrait requires to adapt default paramet
22. s lt Abort on the pipette to abort the program www integra biosciences com 13 VIAFLO ASSIST Operating instructions VOO Operation 4 2 Setting up a program 4 2 1 Overview pipetting modes The table below shows pipetting modes which can be performed automatically in combination with VIAFLO ASSIST All modes are accessed from the ASSIST Menu of a VIAFLO II Electronic Pipette In each mode up to 10 different programs can be created and stored Serial Dilution Allows aspirating a transfer volume followed by a mix Columns and Mix Cycles are tracked on the display Repeat Dispense Allows dispensing multiple aliquots of the same volume without refilling the tips after each dispense for fast microplate filling and processing Variable Dispense Allows dispensing multiple aliquots of different volumes Custom Allows to create and store multi stepped user defined pipetting protocols Serial Dilution Repeat Dispense Variable Dispense Use the Touch wheel to scroll to your desired pipetting mode and Custom press OK NOTE If no Bluetooth connection is available you can also use the offline mode to create a new program Live teaching of pipetting heights is not possible in offline mode Press New D to create a new program of the selected mode Your are prompted to enter a name BCDEFGHI J Pee ug Use the Touch wheel to select characters and press OK Once UVWAY2 H finished press to save the name The
23. spiration e Use the Left and gt Right arrow keys to move the plate sledge into position so that the GripTips target above the center of any wells Press the A Up and WV Down keys to position the GripTips to the desired dis pense height and Press OK on the pipette Alternatively if the proper height is known use the Touch wheel to enter it directly and press OK Height End Set the height for the last aspiration step Asp Speed Setthe aspiration speed 1 low 10 fast Mixing If required set a mixing step before aspiration Press OK to toggle between ON green tick and OFF red cross default f ON define Mix volume Mix Speed and number of Mix Cycles 18 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Operation Dispense options Option Description The First Dispense is discarded back to the source immediately after aspiration A First Dispense volume should be selected to improve accuracy and precision Suggested volume at least 5 of the pipette s maximum volume Press OK to toggle between ON green tick default and OFF red cross If ON define the First Dispense volume Set the total number of dispensing steps First Column Define the column row where dispensing of the first volume should be or First Row started Dispense Sets the volume to be dispensed in each well The total aspiration volume is calculated automatically The pipette cannot be overfilled
24. sting the plate alignment 12 3 7 Charging the battery of the VIAFLO II Electronic Pipette 12 Operation Ack RUNNING Program slice 13 4 2 Setting up a program esse rrereseeerennssnsnes 14 4 2 1 Overview pipetting modes 14 4 2 2 General parameters of all pipetting modes 15 4 2 9 Serial dilution mode euer rp rale 16 4 2 4 Repeat dispense mode 18 4 2 5 Variable dispense mode 20 4 2 6 Custom program mMode 21 4 3 Modify existing programs nn 22 4 4 Troubleshooting LS E m TET 23 www integra biosciences com 3 VIAFLO ASSIST Operating instructions V00 Chapter 5 Chapter 6 Chapter 7 Maintenance Ol SIS CAIN GS sites as ated AN a ter ini 24 o2 DECOMAMINATION 22e ti ne a can ce sed 24 9 9 R Ires M TK nn nee ti ne 24 Technical Data 6 1 Environmental conditions i 25 6 2 Specification of the device 25 Accessories 7 1 Accessories for VIAFLO Electronic Pipettes 26 7 2 Accessories for VIAFLO ASSIST 26 Ko CONSUMADI S seneca ii 27 INTEGRA Biosciences AG VIAFLO ASSIST Operating instructions VOO Introduction 1 Introduction These operating instructions contain all the information required for installation operation and maintenance of the VIAFLO ASSIST This chapter informs about the symbols used in these operating instruc
25. te sledge into position so that the pipette tips target above the center of any wells Press OK to display the setting dial Press the A Up and V Down keys to position the GripTips to the desired dispense height and Press OK on the pipette Alternatively if the proper height is known use the Touch wheel to enter it directly and press OK Set the mixing volume after dispensing It does not affect the transfer volume Mix Speed Set the mixing speed 1 low 10 fast Mix Cycles Set the number of mixes per well 1 30 Last Dispense options Option Description Choose the destination where the last dispense should be purged e Reservoir the reagent reservoir serves as waste position e Tube Strip for PCR tubes e Tip last dispense remains in the GripTips and is purged manually e Column 1 24 or Row A P the last dispense is dispensed in the selected column row of the plate Disp Speed Set speed uniquely for last dispense 1 low 10 fast to position the GripTips at the correct blow out height and press OK e Alternatively use the Touch wheel to enter the height directly if known and press OK BlowOutat The Blowout is following the last dispense and expels extra air to discharge residual liquid from the tips It should be set below the liquid level for best results e Adjust the blow out height If the last dispense is performed on a container located on the plate sledge use the Left and gt R
26. th INTEGRA Biosciences AG Neither the complete documentation nor parts thereof may be reproduced in any way or stored and processed using electronic media or distributed in any other way without the written consent of INTEGRA Biosciences AG This operating instruction manual has part number 127950 the version is VOO It applies to firmware version 1 0 or higher until a newer revision is released VIAFLO VIALINK and GripTip are trademarks of INTEGRA Biosciences Hudson NH Manufacturer INTEGRA Biosciences AG INTEGRA Biosciences Corp CH 7205 Zizers Switzerland Hudson NH 03051 USA T 41 81 286 95 30 T 1 603 578 5800 F 41 81 286 95 33 F 1 603 577 5529 info integra biosciences com www integra biosciences com Customer service Please contact your local INTEGRA Biosciences representative To find out name and address of your local representative go to www integra biosciences com Further information and operating instructions in other languages are available on www integra biosciences com or on request info integra biosciences com www integra biosciences com 29
27. tions the intended use of the VIAFLO ASSIST and the general safety instructions 1 1 Symbols used The operating instructions specifically advise of residual risks with the following symbols WARNING This safety symbol warns against hazards that could result in injury It also indicates hazards for machinery materials and the environment It is essential that you follow the corresponding precautions CAUTION This symbol cautions against potential material damage or the loss of data in a microprocessor controller Follow the instructions NOTE This symbol identifies important notes regarding the correct operation of the device and labour saving features 1 2 Intended use VIAFLO ASSIST has been designed for use in a laboratory With a VIAFLO II electronic multichannel pipette attached it performs pipetting operations in microplates automatically NOTE Only VIAFLO II multichannel pipettes as of serial number 600 000 and firmware version 3 00 or higher can be used with VIAFLO ASSIST The VIAFLO pipette needs its own Bluetooth module which can be ordered separately part no 4221 VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven pipettes They are used for aspirating and dispensing liquids in the volume range of 0 5 1250 yl using GripTip pipette tips Please refer to the VIAFLO II Electronic Pipettes operating instructions for more detailed informations on www integra biosciences com www
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