Sample & Assay Technologies TissueLyser LT User Manual
Contents
1. 2009 TissueLyser LT User Manual For disruption of up to 12 biological samples QIAGEN Sample amp Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies enabling the isolation and detection of contents of any biological sample Our advanced high quality products and services ensure success from sample to result QIAGEN sets standards in Purification of DNA RNA and proteins Nucleic acid and protein assays microRNA research and RNAi Automation of sample and assay technologies Our mission is to enable you to achieve outstanding success and breakthroughs For more information visit www giagen com Contents Contents 1 Safety Information 5 1 1 Proper use 5 1 2 Electrical safety 6 1 3 Environment 7 1 4 Biological safety 8 1 5 Chemicals 9 1 6 Waste disposal 9 1 7 Mechanical hazards 10 1 8 Hot surface 10 1 9 Noise hazard 10 2 Introduction 11 2 1 About this user manual 11 2 2 Intended use of the TissueLyser LT 11 3 General Description 13 3 1 TissueLyser LT principle 13 3 2 Features of the TissueLyser LT system 13 3 3 Applications 15 4 Installation Procedures 16 4 1 Unpacking the TissueLyser LT 16 4 2 Installation of the TissueLyser LT Adapter 16 4 3 Transporting the TissueLyser LT 17 4 4 Installation requirements 17 4 5 Testing the TissueLyser LT 17 5 Operating Procedures 18 5 1 Sample disruption 18 5 2 Changing the2. Sample leaks out of the sample tubes a Lid of TissueLyser LT Tighten the lid securely by screwing the knob on Adapter loose top of the lid clockwise b Grinding beads too Only use grinding beads recommended in the heavy TissueLyser LT Handbook c Wrong sample tubes Only use sample tubes recommended in this used user manual see page 14 Other tubes may not be strong enough to withstand the impact of the grinding beads during sample disruption d TissueLyser LT Adapter Cool down the TissueLyser LT Adapter and the and or sample tubes sample tubes on dry ice or in a freezer not cooled with liqud exceeding 80 C If the adapter or the sample nitrogen tubes are cooled in liquid nitrogen the sample tubes may become brittle and crack during sample disruption TissueLyser LT User Manual 05 2009 23 Appendix Technical data Environmental conditions Voltage 100 240 V AC 50 60 Hz external 24 V power supply designed for wide range of input voltages maximum power consumption approx 30 W Place of For indoor use only operation Air 5 40 C 41 104 temperature Relative Maximum 80 humidity for temperatures up to 31 C linearly decreasing down to a relative humidity of 50 at 40 C Altitude Up to 2000 m 6500 ft above mean sea level MSL Contamination 2 IEC 664 level Mechanical data Dimensions Height 280 mm Width 150 mm Depth 270 mm Mass Approx 7 35 kg net app
3. 1 5 Chemicals WARNING Hazardous chemicals W7 AN Some chemicals used with the TissueLyser LT may be hazardous or may become hazardous after completion of the sample disruption process Always wear safety glasses gloves and a lab coat The responsible body e g laboratory manager must take the necessary precautions to ensure that the surrounding workplace is safe and that TissueLyser LT operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDSs OSHA ACGIH or COSHH documents Venting for fumes and disposal of wastes must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America t ACGIH American Conference of Government Industrial Hygienists United States of America COSHH Control of Substances Hazardous to Health United Kingdom Toxic fumes If working with volatile solvents toxic substances etc you must provide an efficient laboratory ventilation system to remove vapors that may be produced WARNING Toxic fumes w8 AN Do not use bleach to clean or disinfect used labware Bleach in contact with salts from the buffers used can produce toxic fumes 1 6 Waste disposal Used labware such as sample tubes may contain hazardous chemicals or infectious agents
4. 800 930 425 Ireland Orders 1800 555 049 Fax 1800 555 048 Technical 1800 555 061 Italy Orders 02 33430 420 Fax 02 33430 426 Technical 800 787980 Japan Telephone 03 6890 7300 Fax 03 5547 0818 Technical 03 6890 7300 Korea South Orders 1544 7145 Fax 1544 7146 Technical 1544 7145 Luxembourg Orders 8002 2076 Fax 8002 2073 Technical 8002 2067 Mexico Orders 01 800 7742 639 Fax 01 800 1122 330 Technical 01 800 7742 639 The Netherlands Orders 0800 0229592 Fax 0800 0229593 Technical 0800 0229602 Norway Orders 800 18859 Fax 800 18817 Technical 800 18712 Singapore Orders 65 67775366 Fax 65 67785177 Technical 65 67775366 Spain Orders 91 630 7050 Fax 91 630 5145 Technical 91 630 7050 Sweden Orders 020 790282 Fax 020 790582 Technical 020 798328 Switzerland Orders 055 254 22 11 Fax 055 254 22 13 Technical 055 254 22 12 D 3 UK Orders 01293 422 911 Fax 01293 422 922 Technical 01293 422 999 1 USA Orders 800 426 8157 Fax 800 718 2056 Technical 800 DNA PREP 800 362 7737 QIAGEN EN Sample amp Assay Technologies
5. LT be correctly grounded earthed The power supply should be plugged into an AC power outlet that has a ground earth connection 4 5 Testing the TissueLyser LT After carrying out the installation described in Sections 4 1 4 4 and before using the TissueLyser LT for the first time check that the instrument functions properly by following the procedure described in Section 5 1 page 18 TissueLyser LT User Manual 05 2009 17 Operating Procedures 5 Operating Procedures This section describes how to operate the TissueLyser LT system Before proceeding you should familiarize yourself with the features of the TissueLyser LT by referring to Section 3 pages 13 15 5 1 Sample disruption The procedure below describes how to disrupt samples using the TissueLyser LT For detailed information about sample disruption and protocols see the TissueLyser LT Handbook 1 Make sure that the TissueLyser LT Adapter is securely installed on the piston of the TissueLyser LT see Section 4 2 page 16 WARNING Moving parts W10 AN Damage or personal injury can occur if the TissueLyser LT Adapter is not securely attached 2 Make sure that the power supply is connected to the TissueLyser LT and to a power outlet 3 Switch on the TissueLyser LT using the power switch The display lights will flash 4 Using the appropriate or keys the keypad set the disruption time and the oscillation frequency For
6. TissueLyser LT User Manual 05 2009 Introduction 2 Introduction Thank you for choosing the TissueLyser LT system We are confident it will become an integral part of your laboratory Before using the TissueLyser LT it is essential you read this user manual carefully and pay particular attention to the safety information The instructions and safety information in the user manual must be followed to ensure safe operation of the TissueLyser LT and to maintain the TissueLyser LT in a safe condition 2 1 About this user manual This user manual provides information about the TissueLyser LT in the following sections Safety Information Introduction General Description Installation Procedures Operating Procedures Maintenance Procedures e Troubleshooting Guide Appendices The appendices contain the following Technical data E Warranty terms 2 2 Intended use of the TissueLyser LT The TissueLyser LT is intended for molecular biology applications This product is neither intended for the diagnosis prevention or treatment of a disease nor has it been validated for such use either alone or in combination with other products Therefore the performance characteristics of the product for clinical use i e diagnostic prognostic therapeutic or blood banking are unknown TissueLyser LT User Manual 05 2009 11 Requirements for TissueLyser LT users Introduction This table covers the general level of com
7. failure the TissueLyser LT does not automatically restart upon restoration of power case of excess current the TissueLyser LT automatically switches itself off 1 3 Environment Operating conditions WARNING Explosive atmosphere w4 A The TissueLyser LT is not designed for use in an explosive atmosphere WARNING Risk of explosion w5 A The TissueLyser LT is intended for use with reagents and substances supplied with QIAGEN kits Use of other reagents and substances may lead to fire or explosion CAUTION Damage to the instrument C2 AN Direct sunlight may bleach parts of the instrument and cause damage to plastic parts The TissueLyser LT must be located out of direct sunlight The TissueLyser LT operates correctly under the following conditions E Indoors E Air must not contain any electrically conductive dust If the TissueLyser LT is operated in an inert gas atmosphere increased brush sparking on the carbon brushes of the motor may occur reducing the lifetime of the carbon brushes TissueLyser LT User Manual 05 2009 7 Safety Information _ _ oI SE If the TissueLyser LT is operated in a vacuum problems with the ball bearings motor and electronics may occur 1 4 Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious Use safe laboratory procedures as outlined in public
8. from the sample disruption process Such wastes must be collected and disposed of properly according to local safety regulations For information on how to dispose of the TissueLyser LT see Appendix A page 24 TissueLyser LT User Manual 05 2009 9 Safety Information 1 7 Mechanical hazards WARNING Moving parts w9 AN Before installing the TissueLyser LT Adapter switch off the TissueLyser LT at the power switch Avoid contact with the TissueLyser LT Adapter during operation of the TissueLyser LT Do not leave the TissueLyser LT unattended during operation The instrument may creep along the laboratory bench WARNING Moving w10 AN Damage or personal injury can occur if the TissueLyser LT Adapter is not securely attached 1 8 Hot surface WARNING Hot surface w11 AN The TissueLyser LT Adapter may become very hot after high speed shaking The high temperature may also lead to positive pressure within the tubes of the adapter with a subsequent risk of sample splashing Always wear safety glasses gloves and a lab coat Do not operate the TissueLyser LT for longer than 10 minutes without pausing at regular intervals to allow the instrument to cool down Check the TissueLyser LT Adapter and tighten if necessary 1 9 Noise hazard WARNING Noise hazard w12 AN Wear ear protection to protect ears from noise levels of 85 dB A or above 10
9. optimal disruption time and oscillation frequency see the TissueLyser LT Handbook If testing the TissueLyser LT use a disruption time of a few seconds and an oscillation frequency of approx 50 Hz Note Disruption time is displayed either as hours and minutes or as minutes and seconds To change the setting see Section 5 2 page 19 5 Press the Start key on the keypad The TissueLyser LT will now operate at the frequency specified in step 4 In addition the timer on the TissueLyser LT will count down the disruption time i e the timer shows the time remaining WARNING Moving parts W9 AN Avoid contact with the TissueLyser LT Adapter during operation of the TissueLyser LT Do not leave the TissueLyser LT unattended during operation The instrument creep along the laboratory bench 18 TissueLyser LT User Manual 05 2009 Operating Procedures 6 When the timer reaches zero the TissueLyser LT stops operating To stop the TissueLyser LT before the timer reaches zero press the Stop key on the keypad To restart the TissueLyser LT from where the timer stopped press the Start key 7 When the TissueLyser LT is no longer in operation switch it off at the power switch 5 2 Changing the timer settings The timer on the TissueLyser LT displays the disruption time either as hours and minutes or as minutes and seconds The TissueLyser LT is delivered with the timer set to display minu
10. LT is supplied with a warranty that lasts for 12 months starting from the date of shipment The warranty includes all repairs due to mechanical breakdown 6 1 Regular maintenance The TissueLyser LT should be wiped after use using a soft cloth moistened with a suitable cleaning agent see below Be sure to switch off the TissueLyser LT at the power switch and unplug the power adapter from the power socket before cleaning If the TissueLyser LT is switched on there may be a risk of the instrument inadvertently starting during cleaning The components of the TissueLyser LT Adapter should be cleaned after use using a suitable cleaning agent see below rinsed with distilled water and then dried using paper towels If the TissueLyser LT is contaminated with infectious material it should be decontaminated The TissueLyser LT should also be decontaminated before shipping and a decontamination certificate must be completed to certify that the instrument has been decontaminated 20 TissueLyser LT User Manual 05 2009 Maintenance Procedures Cleaning agents The following disinfectants and detergents are recommended for cleaning the TissueLyser LT Note If you want to use disinfectants different from those recommended ensure that their compositions are similar to those described below General cleaning Mild detergents 70 ethanol Disinfection Ethanol based disinfectants can be used for disinfection of surfaces e
11. LY EXCLUDED TO THE FULLEST EXTENT PERMITTED BY LAW NOTE SOME STATES DO NOT PERMIT DISCLAIMERS OF IMPLIED WARRANTIES SO THIS LIMITATION MAY NOT APPLY TO YOU WITH THE EXCEPTION OF THE ABOVE REFERENCED REPAIR OR REPLACEMENT REMEDY QIAGEN SHALL HAVE NO OBLIGATION OR LIABILITY OF ANY NATURE WHATSOEVER WITH RESPECT TO THE QIAGEN INSTRUMENTATION WHETHER ARISING IN CONTRACT TORT STRICT LIABILITY OR OTHERWISE INCLUDING BUT NOT LIMITED TO LIABILITY FOR INDIRECT CONSEQUENTIAL INCIDENTAL AND OR SPECIAL PUNITIVE MULTIPLE AND OR EXEMPLARY DAMAGES AND OR OTHER LOSSES INCLUDING LOSS OF USE LOST REVENUES LOST PROFITS AND DAMAGE TO REPUTATION EVEN IF SUCH DAMAGES WERE FORESEEN OR FORSEEABLE OR WERE BROUGHT TO QIAGEN S ATTENTION IN NO EVENT SHALL QIAGEN S LIABILITY TO YOU EXCEED THE PURCHASE PRICE OF THE PRODUCT Liability clause QIAGEN shall be released from all obligations under its warranty in the event repairs or modifications are made by persons other than its own personnel except in cases where the Company has given its written consent to perform such repairs or modifications All materials replaced under this warranty will be warranted only for the duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices interfacing devices and associated software will be warranted only for the period offered by the ori
12. age contact the transporter of the package Lift the TissueLyser LT out of the packaging with both hands below the base of the instrument After unpacking the TissueLyser LT check that the following items are supplied TissueLyser LT with power supply Power cables for different countries Allen key TissueLyser LT User Manual TissueLyser LT Handbook If anything is missing contact QIAGEN Technical Services Check that the TissueLyser LT is not damaged If anything is damaged contact QIAGEN Technical Services Make sure that the TissueLyser LT has equilibrated to ambient temperature before operating it Retain the package in case you need to transport the TissueLyser LT in the future Using the original package minimizes damage during transportation of the TissueLyser LT 4 2 Installation of the TissueLyser LT Adapter The TissueLyser LT Adapter is not included in the package and has to be ordered separately cat no 69980 Attach the TissueLyser LT Adapter to the TissueLyser LT as follows Screw the base of the adapter onto the piston of the TissueLyser LT Using the Allen key securely tighten the screw on top of the adapter base onto the piston Place the insert with aluminum tube holders into the base Place the lid of the adapter over the insert Screw the knob of the lid until it is securely attached to the base of the TissueLyser LT Adapter WARNING Moving parts W10 AN Damage or persona
13. ance Procedures WARNING Toxic fumes w8 AN Do not use bleach to clean or disinfect used labware Bleach in contact with salts from the buffers used can produce toxic fumes CAUTION Damage to the instrument C3 AN Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the TissueLyser LT CAUTION Damage to the instrument C1 AN Avoid spilling water or chemicals onto the TissueLyser LT Damage caused by water or chemical spillage will void your warranty 22 TissueLyser LT User Manual 05 2009 Troubleshooting Guide 7 Troubleshooting Guide 7 1 General troubleshooting Comments and suggestions No display Power supply not Connect the TissueLyser LT to the power supply connected power Switch on the TissueLyser LT using the power switch in off position switch TissueLyser LT slows down TissueLyser LT Adapter Press the Stop key Reduce the load in the is overloaded TissueLyser LT Adapter and start again b Piston has overheated Reduce the load in the TissueLyser LT Adapter and expanded making allow the TissueLyser LT to cool down movement sluggish TissueLyser LT stops a Drive has overheated Allow the TissueLyser LT to cool down b Drive is blocked Remove all possible obstacles that may block the movement of the TissueLyser LT Adapter If the error persists contact QIAGEN c Speed sensor defective Contact QIAGEN
14. ations such as Biosafety in Microbiological and Biomedical Laboratories HHS www cdc gov od ohs biosfty biosfty htm Samples Samples may contain infectious agents You should be aware of the health hazard presented by such agents and should use store and dispose of such samples according to the required safety regulations WARNING Samples containing infectious agents w6 Some samples used with the TissueLyser LT may contain infectious agents Handle such samples with the greatest of care and in accordance with the required safety regulations Always wear safety glasses 2 pairs of gloves and a lab coat The responsible body e g laboratory manager must take the necessary precautions to ensure that the surrounding workplace is safe and that TissueLyser LT operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Material Safety Data Sheets MSDSs or OSHA ACGIH or COSHH documents Venting for fumes and disposal of wastes must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America t ACGIH American Conference of Government Industrial Hygienists United States of America COSHH Control of Substances Hazardous to Health United Kingdom 8 TissueLyser LT User Manual 05 2009 Safety Information
15. be attached to the piston of the TissueLyser LT In the middle of the figure is the insert of the TissueLyser LT Adapter which contains 12 aluminum tube holders to hold 2 ml tubes At the top of the figure is the lid of the TissueLyser LT Adapter which contains a knob to securely screw the lid onto the base of the TissueLyser LT Adapter Sample tubes The TissueLyser LT Adapter is used with Sample Tubes RB 2 ml cat no 990381 from QIAGEN The tubes can be discarded after use which prevents cross contamination and provides time savings as there is no need to clean tubes after disrupting samples In addition there is no cross contamination during sample disruption as each tube is securely sealed with its own lid 14 TissueLyser LT User Manual 05 2009 General Description Power supply The 24 V power supply which plugs into the TissueLyser LT enables connection of the TissueLyser LT to a power outlet 3 3 Applications The TissueLyser LT enables disruption and homogenization of Human and animal tissues Human and animal cells Plant tissues Yeast Gram positive and gram negative bacteria For information about specific applications and protocols for sample disruption see the TissueLyser LT Handbook TissueLyser LT User Manual 05 2009 15 Installation Procedures 4 Installation Procedures 4 1 Unpacking the TissueLyser LT Before unpacking the TissueLyser LT check whether the package is damaged In case of dam
16. g 25 g ethanol and 35 g 1 propanol per 100 g liquid or Mikrozid Liquid Schilke amp Mayr GmbH cat no 109160 Disinfectants based glyoxal and quaternary ammonium salt can be used for submerging the TissueLyser LT Adapter e g 10 g glyoxal 12 g lauryldimethylbenzylammonium chloride 12 g myristyldimethylbenzylammonium chloride 5 15 nonionic detergent per 100 g liquid Lysetol AF Gigasept Instru AF in Europe cat no 107410 or DECON QUAT 100 Veltek Associates Inc in the USA cat no DQ100 06 167 01 Removal of RNase contamination RNaseZap RNase Decontamination Solution Ambion Inc cat no AM9780 can be used for cleaning surfaces and submerging the TissueLyser LT Adapter Removal of nucleic acid contamination DNA ExitusPlus AppliChem cat A7089 0100 can be used for cleaning surfaces and submerging the TissueLyser LT Adapter General instructions E Do not use spray bottles to spray cleaning or disinfectant liquids onto surfaces of the TissueLyser LT M If solvents or saline acidic or alkaline solutions are spilt on the TissueLyser LT wipe the spilt liquid away immediately Follow manufacturer s safety instructions for handling cleaning agents Follow manufacturer s instructions for soaking time and concentration of cleaning agents exposure for longer than the recommended soaking time can damage the instrument TissueLyser LT User Manual 05 2009 21 Mainten
17. ginal manufacturer of these products Representations and warranties made by any person including representatives of QIAGEN which are inconsistent or in conflict with the conditions in this warranty shall not be binding upon the Company unless produced in writing and approved by an officer of QIAGEN 26 TissueLyser LT User Manual 05 2009 Trademarks QIAGEN QIAGEN Group DNA ExitusPlus AppliChem Gigasept Lysetol Mikrozid Schiilke amp Mayr GmbH DECON QUAT Veltek Associates Inc RNaseZap Ambion Inc 2009 QIAGEN all rights reserved ea www qiagen com Australia Orders 03 9840 9800 Fax 03 9840 9888 Technical 1 800 243 066 Austria Orders 0800 28 10 10 Fax 0800 28 10 19 Technical 0800 28 10 11 Belgium Orders 0800 79612 Fax 0800 79611 Technical 0800 79556 Brazil Orders 0800 557779 Fax 55 11 5079 4001 Technical 0800 557779 Canada Orders 800 572 9613 Fax 800 713 5951 Technical 800 DNA PREP 800 362 7737 China Orders 0086 21 3865 3865 Fax 0086 21 3865 3965 Technical 800 988 0325 800 988 0327 Denmark Orders 80 885945 Fax 80 885944 Technical 80 885942 Finland Orders 0800 914416 Fax 0800 914415 Technical 0800 914413 France Orders 01 60 920 926 Fax 01 60 920 925 Technical 01 60 920 930 Offers 01 60 920 928 Germany Orders 02103 29 12000 Fax 02103 29 22000 Technical 02103 29 12400 Hong Kong Orders 800 933 965 Fax 800 930 439 Technical
18. l injury can occur if the TissueLyser LT Adapter is not securely attached 16 TissueLyser LT User Manual 05 2009 Installation Procedures 4 3 Transporting the TissueLyser LT Carry the TissueLyser LT with both hands below the base of the instrument Do not carry the TissueLyser LT by holding the TissueLyser LT Adapter 4 4 Installation requirements Site requirements Place the TissueLyser LT on a flat stable surface and ensure that there is sufficient space around and under the instrument for ventilation Leave some space to the right of the instrument for the power switch and power supply The TissueLyser LT is for indoor use only under the following conditions Room temperature within the range of 5 40 C 41 104 Up to 2000 6500 ft above mean sea level MSL E Maximum relative humidity of 80 for temperatures up to 31 C linearly decreasing down to a relative humidity of 50 at 40 C Contamination level 2 664 Power requirements The power line to the TissueLyser LT should be voltage regulated and surge protected The power supply of the TissueLyser LT is compatible with voltages of 100 240 V Make sure that the voltage rating of the TissueLyser LT power supply is compatible with the AC voltage available at the installation site Grounding requirements To protect operating personnel the National Electrical Manufacturers Association NEMA recommends that the TissueLyser
19. nformation for scheduling collection of the electronic waste or to provide you with an individual quote TissueLyser LT User Manual 05 2009 25 Appendix Warranty statement Thank you for your purchase of QIAGEN instrumentation Your instrument has been carefully tested to ensure optimum operating efficiency and reproducibility of results QIAGEN warrants that all new instrumentation manufactured by QIAGEN will correspond to the product specifications and be free from defects in workmanship and materials for a period of twelve 12 months from the original date of shipment see page 20 Repair or replacement of defective parts will be provided to the purchaser during this time period provided the QIAGEN instrumentation is operated under conditions of normal and proper use but not for damage caused by the customer If any part or subassembly proves to be defective it will be repaired or replaced at QIAGEN s sole option subsequent to inspection at the factory or in the field by an authorized factory representative provided that such defect manifested under normal and proper use Limitation of warranties and remedies THE FOREGOING WARRANTY IS QIAGEN S SOLE AND EXCLUSIVE WARRANTY AND REPAIR OR REPLACEMENT OF DEFECTIVE PARTS IS THE SOLE AND EXCLUSIVE REMEDY THERE ARE NO OTHER WARRANTIES OR GUARANTEES EXPRESS OR IMPLIED THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESS
20. petence and training necessary for transportation installation use maintenance and servicing of the TissueLyser LT Training and Task Personnel experience Transportation No special No special Installation routine use and maintenance Servicing requirements Laboratory technicians or equivalent QIAGEN or an authorized agent service technicians requirements Appropriately trained and experienced personnel 12 TissueLyser LT User Manual 05 2009 General Description 3 General Description The TissueLyser LT is a compact bead mill designed for rapid efficient and flexible disruption of up to 12 samples at the same time A wide variety of biological samples can be processed including human animal and plant tissues Each sample is simultaneously disrupted and homogenized by high speed shaking with a bead in a sealed tube bead milling 3 1 TissueLyser LT principle Efficient sample disruption is a prerequisite for nucleic acid and protein purification procedures Incomplete sample disruption can lead to significantly reduced yields and can increase the risk of clogging when using purification columns or magnetic particles The TissueLyser LT thoroughly disrupts and simultaneously homogenizes biological samples in the presence of lysis buffer Plant tissues can alternatively be disrupted and homogenized in the absence of lysis buffer Tubes containing sample and bead are placed in the Tiss
21. rial damage w1 AN Improper use of the TissueLyser LT may cause personal injuries or damage to the instrument The TissueLyser LT should only be operated by qualified personnel who have been appropriately trained Servicing of the TissueLyser LT should only be performed by QIAGEN or an authorized agent service technicians The TissueLyser LT should only be used for the applications described in the TissueLyser LT Handbook The TissueLyser LT should only be used in combination with the TissueLyser LT Adapter Do not continue to use the TissueLyser LT or TissueLyser LT Adapter if either is damaged Perform the maintenance as described in Section 6 QIAGEN charges for repairs that are required due to incorrect maintenance TissueLyser LT User Manual 05 2009 5 Safety Information CAUTION Damage to the instrument C1 AN Avoid spilling water or chemicals onto the TissueLyser LT Damage caused by water or chemical spillage will void your warranty WARNING Risk of personal injury and material damage 2 AN Do not attempt to move the TissueLyser LT during operation In case of emergency switch off the TissueLyser LT at the power switch and unplug the power supply from the power outlet 1 2 Electrical safety WARNING Electrical hazard w3 AN Any interruption of the protective conductor earth ground lead inside or outside the TissueLyser LT or disconnection of the protective conduc
22. rox 9 kg gross Oscillation 15 50 Hz frequency Noise level lt 85 dB A 24 TissueLyser LT User Manual 05 2009 Appendix Waste Electrical and Electronic Equipment WEEE This section provides information about disposal of waste electrical and electronic equipment by users in the European Union The European Directive 2002 96 EC on WEEE requires proper disposal of electrical and electronic equipment when it reaches its end of life The crossed out wheeled bin symbol see below indicates that this product must not be disposed of with other waste it must be taken to an approved treatment facility or to a designated collection point for recycling according to local legislation The separate collection and recycling of waste electronic equipment at the time of disposal helps to conserve natural resources and ensures that the product is recycled in a manner that protects human health and the environment QIAGEN accepts its responsibility in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN provides free recycling of its WEEE marked electronic equipment in Europe If a replacement product is not being purchased from QIAGEN recycling can be provided upon request at additional cost To recycle electronic equipment contact your local QIAGEN sales office for the required return form Once the form is submitted you will be contacted by QIAGEN either to request follow up i
23. tes and seconds To change the time format follow the procedure below 1 Press and hold the Stop key for 5 seconds The timer will go into setup mode 2 Using the and keys select O to display disruption time as hours and minutes or select 1 to display disruption time as minutes and seconds 3 Press the Stop key to save your selection Note The TissueLyser LT and TissueLyser LT Adapter reach very high temperatures after 10 minutes of operation When using long disruption times we strongly recommend stopping the instrument at regular intervals to allow it to cool down TissueLyser LT User Manual 05 2009 19 Maintenance Procedures 6 Maintenance Procedures Perform the following regular maintenance procedures i e after each daily use to ensure reliable operation of the TissueLyser LT CAUTION Damage to the instrument C3 AN Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the TissueLyser LT If solvents or saline acidic or alkaline solutions are spilt on the TissueLyser LT wipe them away immediately Do not autoclave any part of the TissueLyser LT or the TissueLyser LT Adapter WARNING Risk of electric shock w13 AN Do not open any panels on the TissueLyser LT Risk of personal injury and material damage Only perform maintenance thot is specifically described in this user manual Servicing The TissueLyser
24. timer settings 19 6 Maintenance Procedures 20 6 1 Regular maintenance 20 7 Troubleshooting Guide 23 7 1 General troubleshooting 23 TissueLyser LT User Manual 05 2009 3 Contents Appendix A 24 Technical data 24 Waste Electrical and Electronic Equipment WEEE 25 Appendix B 26 Warranty statement 26 Liability clause 26 4 TissueLyser LT User Manual 05 2009 Safety Information 1 Safety Information Before using the TissueLyser LT system it is essential that you read this user manual carefully and pay particular attention to the safety information The instructions and safety information in the user manual must be followed to ensure safe operation of the TissueLyser LT and to maintain the TissueLyser LT in a safe condition The following types of safety information appear throughout this user manual WARNING The term WARNING is used to inform you about situations A that could result in personal injury to you or other persons Details about these circumstances are given in a box like this one CAUTION The term CAUTION is used to inform you about situations that A could result in damage to the TissueLyser LT or other equipment Details about these circumstances are given in a box like this one The advice given in this manual is intended to supplement not supersede the normal safety requirements prevailing in the user s country 1 1 Proper use WARNING Risk of personal injury and mate
25. tor terminal is likely to make the TissueLyser LT dangerous Intentional interruption is prohibited Lethal voltages inside the TissueLyser LT When the TissueLyser LT is connected to line power terminals may be live and opening the TissueLyser LT or removing parts is likely to expose live parts To ensure satisfactory and safe operation of the TissueLyser LT follow the advice below The line power cord must be connected to a line power outlet that has a protective conductor earth ground Do not adjust or replace internal parts of the TissueLyser LT Do not operate the TissueLyser LT with any covers or parts removed M If liquid has spilled inside the TissueLyser LT switch off the TissueLyser LT disconnect it from the power outlet and contact QIAGEN Technical Services 6 TissueLyser LT User Manual 05 2009 Safety Information Ifthe TissueLyser LT becomes electrically unsafe prevent other personnel from operating it and contact QIAGEN Technical Services the TissueLyser LT may be electrically unsafe when Milt or the line power supply appears to be damaged has been stored under unfavorable conditions for a prolonged period Mt has been subjected to severe transport stresses If the TissueLyser LT is not in use switch it off at the power switch If the TissueLyser LT will not be used for a prolonged period of time unplug the power supply from the power outlet In case of power
26. ueLyser LT Adapter and shaken up and down at a very high speed causing the samples to be disrupted and homogenized by the beating and grinding effect of the beads When processing plant tissues or unstabilized animal and human tissues the TissueLyser LT Adapter passively cools the sample tubes during the disruption process to prevent degradation of biomolecules 3 2 Features of the TissueLyser LT system TissueLyser LT This compact instrument provides high speed vertical shaking with oscillation frequencies of 15 50 Hz Power is switched on and off at the power switch located on the right hand side of the instrument Oscillation frequency can be adjusted in steps of 1 Hz using a keypad located on top of the instrument Disruption time up to a maximum of 1 hour 59 minutes can also be set using the keypad TissueLyser LT Adapter The TissueLyser LT Adapter enables the TissueLyser LT to disrupt and homogenize up to 12 samples at the same time The TissueLyser LT Adapter is connected to the piston of the TissueLyser LT and securely holds up to twelve 2 ml tubes Figure 1 For disruption of plant tissues in the absence of lysis buffer or disruption of unstabilized animal and human tissues the TissueLyser LT Adapter can be precooled on dry ice before use TissueLyser LT User Manual 05 2009 13 General Description Figure 1 TissueLyser LT Adapter At the bottom of the figure is the base of the TissueLyser LT Adapter which has to
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