Home

DeltaPix Camera & Software Installation Manual DpxView LE

1. 86 DeltaPix 2007 VI Page DpxView Pro Manual Extended Exposure can also be calculated by right mouse clicking on the extended EXP exposure button lt 7 Anew window will open see figure to the right where it is possible to select the relative exposure time in percent and then press the button Acquire and Process This will make the software automatically capture images with the selected exposure times and combine them afterwards to one image DeltaPix 2007 VI Extended Exposure Relative Exposuretime EXP EIS SIE Se Kli mn Ei d L Le Ki gt GI A Gi Acquire and Frocess G Si Auen Cell Gel GI Le ES A Clove all Sources Close all Sources Aan iD Auto close Sources Bl 8 Page DpxView Pro Manual 7 DpxView Pro AZ Automatic Extended Focus amp Extended Exposure 7 1 DpxView Pro ZA Automatic Z control Racius 10 Create Deformation 1C SS E Coe all oree Rotatior deg Ee Ww Auo che Sources Magrilication 10 a Allow Fesanpliag Smoot A EE E EEEIEE TEE Show Hinks temahe Evtseded Fse Aulometic 2 cotio Bidireztioral aris Device e Skup Standa RSMO Cerriguiation SES lo fal D Gei Evtended Exposure Citable Gabup Extended Exposure Relative Exposuretinie 7 ae Gall Ee elj en Dal kal wl m 1o EECH F HE DeltaPix 2007 VI Ka Acquire and Process x Cl
2. Sample image same image and illumination as before now with Shading Correction enabled Shade Correct Live Preview enables you to see the process of correcting the image If the shading correction file has been saved it can be automatically loaded a program start to make shading correction work immediately Check Settings Options Load Shading Correction file upon program start to make this happen The Shading Correction process uses a lot of CPU resources When not doing Shading Correction on the Live Preview some CPU resources can be saved When running live preview Shading Correction on big images displayed with high frame rate you may exhaust your PC resources 3 4 7 Frame Average Enabling this function will result in display of an averaged P OFF image of the last 2 4 or 8 frames 2 frames d Frames The video frame rate itself will remain unchanged but changes in the scene will be adapted only slowly to the image The advantage will be lower noise in the video image since external noise sources such as flickering illumination will be averaged and thereby decreased 6 frames 3 4 8 Binning Binning adds neighbouring pixels together thereby Ps Off increasing sensitivity The disadvantage is that resolution j Software Binning decreases Red Green This function is especially useful in low light condition for ee Glue focusing and centering the object under observation S SE
3. Infinity X DP 200 DP 252 DP 253 DP 300 DP 450 Cameras The contents of this document may not be copied nor duplicated in any form in whole or in part without prior written consent from DeltaPix DeltaPix makes no warranties as to the accuracy of the information contained in this document or its suitability for any purpose The information in this document is subject to change without notice Copyright 2003 2007 DeltaPix All rights reserved DeltaPix Camera Manual DpxView LE Table of Contents 1 THEDEETAPIX VIEWER sosedu aa Eei aiaa e ante ann aaa aiaa 5 MT Tu VIEW EE 6 3 MAIN MENU eegene 7 2 A FIEMMEN E D 3 1 1 SMS MO EE 7 3 1 2 Pnt SIVA S NOU EE 7 SMS e e te Le 8 3 1 4 Eege 8 Se VIEW MENUS E A E A A 9 d GC ST Ee Lu ET BE H 34 Camera E cssc anaia aai AE aaae aia aiaiai 10 3 4 1 BSS IZ E EEN AE A N E AE A A A E A 10 3 4 2 ele 11 3 4 3 Wila BRCO EE 11 3 4 4 Snapshot Settings for CCD cameras DP 252 DP 253 and DP Ab 11 3 4 5 Bl ck Frame GCOMDESMSATON EE 13 SAG ondan COC OI WEE 14 3 4 7 AIMS AV CAS EE 15 3 4 8 Eelere DE 15 3 4 9 EE 16 23410 Frame Rate Mode E 16 A EE RO e eee eee nee ee eee EE 16 TAI FERO US ele EE 17 Se el Wee e e E 17 3 4 14 Preview Demosaicing EE 17 Ala GOO CONC COM EE 17 SAO FIA EE 17 SAA ROSC N EE 17 35 PRINS dE D 18 3 5 1 KENE 18 3 5 2 A NANGGO ee E E 19 3 5 3 VE MPIC AON EE 22 3 5 4 Hoht Source FreguenGy cesede ene E EE EEE E ETE 22 3 9 9 MEMO EE 23 20 NENEN EE 23 A BUTTO
4. DeltaPix 2007 VI Page DpxView Pro Manual 3 6 4 Image 3 6 4 1 Flip Deskew and Reverse Image Image Effects Color oo Fi Flip can be used to flip the image vertically top to bottom ai Deskew Deskew makes images not straight but rotated by a certain EE degree that you do not know In other words those images 2 have a skew The degree of the rotation might vary from image Rotate to image Shear en Reverse tool is an image where the left and right have Canvas Resize changed places Resize Histogram 3 6 4 2 Rotate Image Rotate Image creates a new image that is a rotated copy of Image Effects Color Post Acquire Proce an existing one It is possible to rotate 90 180 270 degrees Flip clockwise Q or you can select another value as shown paskem below Positive angles rotate clockwise while negative angles Reverse Rotate 90 clockwise rotate counter clockwise shears 180 clockwise Canvas Resize 270 clockwise Resize Any Angle Histogram 3 6 4 3 Shear Image Shear Image creates a new image that is a shear image copy of an existing one Shearing slides one edge of an image along the X or Y axis creating a parallelogram A horizontal direction shear slides an edge along the X axis while a vertical direction shear slides an edge along the Y axis The amount of the shear is controlled by a shear angle For horizontal direction shears x shear is measured re
5. Creating the thumbnail gallery may be time and memory consuming It is recommended not to browse directories containing many images e e e E eg a Cleats w ba am re ZS Za 58 DeltaPix 2007 VI Page DpxView Pro Manual 3 2 4 Open Image Function Pm PR mn Pm mm ee mme e E Sc Eed aes NEE 2 Ss nn tratsr Wals L lizss RS e ee i F oe E re H b e f i d a ee E arar p SR P f cke f ae d P A q E 28 i It is possible to view a larger image from the thumbs by selecting an image from your achieve followed by activating the Open Image button d It is recommended not to have many images open at the same time This may reduce acquisition and processing speed The number of images to keep open depends on the amount of installed RAM in your PC S HCI 3 2 5 Magnifier Function priyew l r l Se i md Settegs Documents Detala imegers imoges Dpr e TA i WENO IM YLI wo sw 8 RK teen ax Cocora Preview Ty inan o Lumgazrzeo Baa PEZI it Bin 200 300 e 400 500 The Magnifier button allows you to zoom with a view as shown in the figure to the right Move the magnifier over a selected object in order to view more details The magnifier zooms in with a value of 100 200 300 400 or 500 3 2 6 Viewfinder Function Die Tun lee SE Chee sel eat Docume aia ad eee eel os TLIC SNE HDLIL R US 1 UM eI get EIER KH
6. Ir Tim e Exposure TEPER Max 500ms Several choices are available in the Max Auto Exposure Time where you can select between 50ms 100ms 500ms 1000ms or No Limit 5 5 White Balance Submenu The Auto entry will toggle the White maaa SS Balance control from Automatic to ikg White Balance i bii One Shot Spot place Spot Manual mode or vice versa One Shot Spo Place Spo Color Target One Shot Spot function gives you the Manual fine tune possibility of using a single spot as reference for the white balance function by placing the crosshatch at the desired reference point in the image This reference can be any color chosen in the Color Target sub menu Basic colors Normally only white should be used unless you are WI EFSI SE very sure of the color you want to set as a reference a ES DE WR ion ff ee DW EER BEE EES BEE EEE EES Custom colors Define Custom Colors gt gt DeltaPix 2006 VI Page 35 DeltaPix Camera Manual DpxView LE With the Manual Fine Tune function the settings can be adjusted by means of three sliders from the Analog Gain and or the Digital Gain Analog Gain Red 1 55 By moving the slider to the right the color values EE increase and by moving it to the left the color Green PU values will decrease i Blue 3 000 It is also possible to set the values to their default values by clicking the left mouse with the cursor positioned abov
7. the resulting image file size is four times smaller than the original size In numbers 960 KB DeltaPix 2006 VI Page 10 DeltaPix Camera Manual DpxView LE 3 4 2 Exposure The Auto entry will toggle the exposure function from Automatic to Manual exposure SE or vice versa Max Auko Exposure Time Max 50ms Auta CIrLE kk A Exposure Max 100ms The Converge entry will SE initially set the exposure S function to Auto until the Mea Em picture has settled leaving the NoLimit exposure function in Manual mode Several choices are available in the Max Auto Exposure Time where you can select between 50ms 100ms 500ms 1000ms or No Limit The Selective Auto Exposure Range is recommended to be closed to 50ms because if there is any change in light source the exposure time will not take a long time to be adjusted from 50ms to xx ms However if the exposure range was selected to No Limit it will take some time to come down from 6s to xx ms 3 4 3 White Balance The Auto entry will toggle the White Balance control from Automatic to Manual mode or vice versa Auto ChrlONM One Shot Spot Place Spot Crit Color Target dl White Balance Manual Fine tune The auto white balance uses the entire image as target The best result is achieved by removing the specimen form the light path and enable auto exposure When the colors have settled disable the auto exposure and insert the speci
8. Ok button to close dialog and accept activate any changes made and the Cancel button to ignore any changes made 3 6 4 5 Resize Resizing the image removes pixels to make it smaller or adds pixels to make it larger You can resize an image after you have created it but doing so may result in loss of image data It is important to select an appropriate resolution Too low a resolution causes pixilation or large pixels that produce coarse output Too high a resolution adds to an image s memory requirements without producing a proportional increase in its quality In the Pixel Dimensions group box enter the new Width and Height values From the drop down list the values are entered as a percent of the original size To constrain the new image dimensions to the image s current proportions mark the Maintain Aspect Ratio check box The aspect width to height ratio defaults to the ratio of the active image Changing the aspect ratio distorts the image by stretching or contracting it in one dimension more than the other The Method box chooses the mathematical technique used to interpolate the existing pixels into a greater or lesser number of pixels as the image changes size Different methods will at times work better or worse depending on the image content Choose from Normal Resample or Bicubic The Bicubic method works best with most images The Resample command is used to increase or decrease the size and or resolutio
9. the picture is not all at kf Shade Correct Live Image exactly the same signal level In general this shading effect is undesirable open Eliminating it is frequently necessary for subsequent processing and especially when Lt nave image analysis or image understanding is the final goal A technique used to remove these kinds of effects is Shading correction or Flat fielding A scaled image division performs removal of the flat field image contribution This process requires acquisition of a flat field image The flat field is an image just showing the illumination no objects must be present Good results are often achieved by doing a slight defocus of the flat field Select the Capture function will open the Shading Correction DeltaPix Camera window i Now prepare Shading Correction Frame Note It may be disirable to do a slightly defocus Press OK when ready Press the button OK will open a new window informing that the Shading Correction Frame has been captured DeltaPix Camera e l Shading Correction Frame has now been captured This image shows very uneven illumination in general the best results are always achieved by using a uniform illumination from the very start Flat field image sample Select the Enable entry will start correcting the image This is a sample image without Shading Correction enabled DeltaPix 2006 VI Page 14 DeltaPix Camera Manual DpxView LE
10. 1 000 Brightness 0 662 Gamma 1 000 a Hue 4 12 Saturation 1 232 gain 1 296 ain AGB K Hue 4172 Saturation 142 gain 1 296 lain AGB gt gt Capture Process DeltaVu Calculation Process The capture process can also be started from the Image Menu 6 2 Output Window Pressing the Outout Window Button will open a new window in which you will be able to view the captured images manipulate and process them upload previously saved images Zoom in on details etc 6 3 Camera Settings Bar The Camera Settings bars enable you to control contrast Contrast 2 364 brightness gamma hue saturation and gain e x a o o Brightness 0 662 E Dragging the slider to the right will increase the values dragging it to 25 the left will decrease the values EE The values can be fine tuned by pressing the up down buttons at 4 ES the right SS E It is also possible to set the Camera Setting Bars to their default values by clicking the left mouse with the cursor positioned above the sliders Saturation 1 42 4 Gain 1 296 Gan AGE gt gt Ka 4 DeltaPix 2006 VI Page 42 DeltaPix Camera Manual DpxView LE 7 Status Bar The status bar shows miscellaneous camera and viewer information Indicates whether a DeltaPix camera is attached or not Progress Bar showing the progress in image capture and Red Green and Blue intensity DeltaVu processing in the c
11. 1 3 Mega Pixels not using the DeltaVu Micro scanning Technology 1280 x 1024 Demosaic The second modes from 1280 x 1024 DeltaVu color to 5120 x 4096 1260x1024 Vu color DeltaVu color permit you to select the resolution from 1 3 Mega Pixels up S560 x 2048 Gir pala to 21 Mega Pixel using the DeltaVu Micro scanning Technology 3640 x S072 Vu color The last two modes have been developed recently allowing you to 5120x4096 wu calor decrease the capture time of a high resolution image approximately to one fourth of the normal DeltaVu color using the DeltaEx color 3840 x 3072 Ex color 2560 x 2048 Ex color 1280 x 1024 DeltaNr color allows you to capture an image with automated Black frame Compensation function 1280 x 1024 Mr color pe hi Pressing the Capture Button will capture a high resolution image at the selected resolution Pressing Camera Setting Button will toggle the camera setting toolbar on and off The Fast Focus Mode Button helps to yield fast image displays with a sensor region 1280 x 1024 The Visualizer Mode Button helps to yield fast image displays with a sensor region 640 x 480 Reducing the sensor area will allow for higher frames rates BAr The Exposure Button toggles between automatic and manual exposure EXP The Exposure Converge Button forces the camera into the Converge mode WB The White Balance Button toggles between automatic and manual white balance The Place Spot Button enables the Spot White Bala
12. DpxView LE 5 18 Zoom Zoom selection enables you to directly select different levels of zoom Also the mouse scroll wheel may be used for the zoom function however it may be needed to select the zoom function manually first if the scroll wheel previously has been assigned to another function oam LOO OU 400K SOU 1600s 3200 Fit to Window By pressing the left mouse button while moving the mouse the active image section i e the viewable part of the capture will be repositioned 5 19 Exit Program This function closes the DeltaPix viewer DeltaPix 2006 VI Exif program Page 40 DeltaPix Camera Manual DpxView LE 6 Camera Toolbar The Camera Toolbar provides you with an easy access to commonly used adjustments on video and image view such as contrast brightness gamma hue saturation and gain The Camera Toolbar can be activated by the Right Click Menu or in the View sub menu in the Main Menu Contrast 1 364 The two upper left buttons in the Camera Toolbar circled in Brightness UE green enable you to select the resolution of the captured still images Gamma 1 000 The digital still image resolution may be Hue 4 172 e a single exposure with 1280 x 1024 pixels 1 3 million a Saturation 142 pixels or e amultiple exposures with 1 1280 x 1024 pixels 2 2560 x 2048 pixels 3 3840 x 3072 pixels 4 5120 x 4096 pixels 1 3 million real pixels Gain 1 256 Gain AGE
13. Infinity X camera contact your local DeltaPix dealer for further instructions Do not try to calibrate the camera without having received proper instructions Backup Calibration will save a copy of the current camera settings Restore Calibration will upload the Backup calibration file to the camera 3 5 2 3 How to upgrade the Infinity X camera DeltaPix is constantly developing new modes for the Infinity X camera Cameras installed at an end user can be upgraded in the field The first time a not up to date camera is used with DeltaPix SU software that supports new modes a message box appears l This Infinity amp can be updated with new modes figure to the right J Please contact your distributer for more information If this message box has appeared then the camera can be updated Use the menu Options Advanced Auto Upgrade Camera EL Settings M Window d Help E l Options When image captured d Maonification 3 w Auto suggest filename Gi Lkbtseutesrtteogugegg oF Prompt when renaming image files FTP Play Sound when image saved or opened High Resolution Image Stamps V Dateie Load Shading Correction file upon program start g pon prog Warn when saving files with non persistent calibration information TWAIN Data Source V w Reset Focus bar when Exposure changes L Advanced Shay on Top Use Trayicon Directs Support b p Calibrate Camera vw Save Profiles as ASCII White Balance a
14. The CPU usage when the Video is Off The CPU usage when the Video is ON 3 6 Help menu File view Image Camera Settings Help The help options are listed below e a Questions and Answers Ctrl Q e Questions and Answers opens the Help File AL Seon menu in which you have access to the ER D i Help shortcuts installation software manual and using the i viewer software document GY About Ctrl H e Support function enables you to write directly to DeltaPix in case you have any technical problems or if you have any question Information about your camera will automatically be included in the mail Please don t hesitate to contact us with any questions or comments you may have e Application info opens a new window including technical data of the camera and the software e www deltapix com is the homepage of DeltaPix This is the place to look for software updates and new camera products e Many functions can be activated by Helo Shortcuts The list can easily be printed or copied to the clipboard DeltaPix 2006 VI Page 23 DeltaPix Camera Manual DpxView LE 4 Button Toolbar The Button Toolbar is located just below the Main Menu The Button Toolbar is a user configurable menu and functions This solution is primarily designed to match your individual applications In many applications you do not need to view a feature you do not use that s typically where DeltaPix software fits in Therefore go to your button men
15. Window Mask filter Blur g Un sharp mask is a technique to enhance the edges of an Moise a image Un sharpen mask tends to show more details in areas Sharpen Unsharp Mask of an image that are normally not visible This is done by Calculations Sharpen sharpening the mid to high contrast edges in the image en Eug without enhancing noise S Edge i This command locates adjacent pixels that have a difference Spatial Filters b in lightness values that you specify and increases their contrast by an amount you specify Foree tocea Force Mono Fo Sharpen command starts with a low value and increase it TrReRINGGe IFS gradually to increase the amount of contrast in the image s pixels Add Open Images 4dd Thumb Images 3 6 5 4 Effects Calculations The Calculations Multiply function will start a new window By Effects Color Post Acquire Process Window typing a factor in the white box the brightness of the image will Blur 3 increase oe e Multiply Sharpen ties Calculations Multiply W S W E og peter Add Bitmaps 3 a Edge Spatial Filters d Force to Color d Force Mona Fo SS A Invert Image Fo 4dd Open Images Idle Ce 4dd Thumb Images Add Bitmaps adds or averages the bitmaps in a list according to their weight factors This helps is to minimize noise and improve low light vision images Please note that it takes few seconds to view the window shown to the right when the Ef
16. an new Image Chr special effects enter or update the entire contents of the image Select Region hel Mone File Properties EXIF Ctrl Alt E Rectangle Insert Calibration Bar Fiz Ellipse Change Default Bar Properties Chri Fiz Round Rectangle Free Hand Frame Wt Animated Frame Static Frame Without Frame You can copy a selection of the entire image and then paste the contents into the same image or another image Selecting the parts of images to edit is an important step in modifying images After you create a selection you can edit it while leaving the rest of the image unchanged The selection can be any shape a rectangle ellipse round rectangle or a free hand shape selections With Freehand click and drag to select the area like making a freehand drawing Use this type for irregular shapes when you want to make a selection quickly 3 6 3 View 3 6 3 1 Normal View View Image Effects Color Post Acquire In Normal View every pixel in the image is represented Normal Chr by one pixel on the screen Ze Chr alt z If the whole picture will not fit in the window scroll bars eebe SET appear so you can move the view about bef Fit Image To Window Ctrl F Pan Window Ctrl P Realize Annotation ChrlLGAEAR Grea and Perimeter Crl 4lk 4 Image Comment CErl AlE 3 6 3 2 Zoom Function Zoom In Out You can change the normal viewing mode by selecting the ae ho 4 Zoom function Move the scroll ba
17. are also indicators of need for external power Power Surge On Hub Port xl Power Surge On Hub Port SC RECH The Infinityes USB 2 0 Camera has malfunctioned and exceeded the The Infinity USE 2 0 Camera has exceeded the power limits A power limite of its hub port You should disconnect the device of its hub port For assistance in solving this problem click this message The location of the device is shown in bold type Gs USB Root Hub 6 ports Unused Port Unused Fort lt Infinity USB 2 0 Camera Unused Fort Unused Fort Unused Fort Recommendation To reenable the port disconnect the hardware and click Reset If you click Close the port will no longer function 54 DeltaPix 2007 VI Page DpxView Pro Manual 3 DpxView Pro Image Management Software The DpxView Pro is an image handling software allowing advanced functions for Zooming annotation measurements locate and maintain images DpxView Pro can create online image galleries with image browsing capabilities The main features of the DpxView Pro are Archive Search Functions Thumbnails Multiple views View in view Annotation Active Zoom Merge images Measuring tools like distance angle area and perimeter 3 1 Initial View In the start menu you will find two icons A DelaPix Viewer LE DpxView LE the standard software and A8 Dpxview Pro DpxView Pro the Still Image Man
18. at low frame rate can be played in accelerated time DeltaPix 2006 VI Page 38 DeltaPix Camera Manual DpxView LE M The recorder is aimed at recording small videos The AVI recorder uses high CPU resources especially at combination of large images e g above 800 x 600 and fast frame rates e g above 10 frames sec If using large images and fast frame rates frames may be dropped resulting in flickering video or shorter play back time than expected 5 10 Main Menu Enables or disables the Main Menu 5 11 Button Toolbar Enables or disables the Button Toolbar 5 12 Auto hide Button Toolbar Use this function to obtain maximum image screen area 5 13 Window Header Enables or disables the Window Header 5 14 Status Bar Enables or disables the Status bar 5 15 Live Image Enables or disables the live image mode 5 16 Tools The Tools entry comprises an overall DICOM DE ini DICOM viewer Viewer with limited functionality The viewer uses the de facto standard for viewing medical images Contrast and brightness can be adjusted by dragging the mouse over the image 5 17 Full screen video preview To obtain maximum screen area for the video image display disable the Statusbar the Windows Header Button Toolbar and enable Auto hide Buttonbar When mouse cursor is positioned at the top of the image the button bar will automatically be shown DeltaPix 2006 VI Page 39 DeltaPix Camera Manual
19. gray white or black that is mixed into the color Zero saturation indicates no hue just gray scale The value component of the HSV space is a measure of its brightness Lightness is the amount of black or white in a color Increasing lightness adds white to the hue Decreasing lightness adds black to the hue 3 6 1 3 Enable Drop File lf this menu item is checked drag and drop of image files are accepted 3 6 1 4 Print and Print Preview Prin enables you to print an image Print Preview displays how the document will print as opposed to how it looks on the screen 3 6 1 5 Import and Export Images Import Function and Export Function permit you to import and export an image in JPEG2000 or DICOM formats from to a file 3 6 1 6 Capture Screen Capture Screen makes a screen shot for copying to printers the clipboard or files Desktop Wallpaper function allows you to set an image selected from the thumbnails as desktop wallpaper on your PC Note that the file format should be a bitmap file Image Info displays information about your image 3 6 1 7 Exit and Camera window Exif function ends the DpxView Pro application Camera Window will shift the view to the live image window 68 DeltaPix 2007 VI Page DpxView Pro Manual 3 6 2 Edit The DpxView Pro Edit View Image Effects Color Post Acquire Process Window software makes it easy Sen Ctrl C to edit nages and apply Faste
20. in brightness as one goes to the edge of the field of view DeltaPix 2006 VI Page 36 DeltaPix Camera Manual DpxView LE In other cases the image might be darker on the left side and lighter on the right side The shading might be caused by non uniform illumination non uniform optics or even dirt and dust on glass lens surfaces In general this shading effect is undesirable Eliminating it is frequently necessary for subsequent processing and especially when image analysis or image understanding is the final goal Select the Capture function will open the Shading DeltaPix Camera Correction window Now prepare Shading Correction Frame Jj Note It may be disirable to do a slightly defocus Press OK when ready Press the button OK will open a new window informing that the Shading Correction Frame has been captured Ce 1 Shading Correction Frame has now been captured DeltaPix Camera Select the Enable entry will start correcting the image Shade Correct Live Preview enables you to see the process of correcting the image 5 8 Camera Settings Toolbar Visible The Camera Setting Toolbar Visible enables or disables the toolbar 5 9 Video Capture Toolbar Visible Recording AVI When recording AVI using the DeltaPix viewer LE or Pro some precautions can be made to ensure high quality of the video e Use as small image as possible 640x480 frames generate mush less data than 1280x1024 e Use as
21. low frame rate as possible recording at 10 or more frames sec may drop frames in the video and make the playback appear faster than intended e Set the camera frame rate close to the recording frame rate E g if recording at 1 frame sec then manually set the camera frame rate to around 2 frames sec See section below or consult your manual on how to do this e Make sure that the PC does not hibernate or in any other way enter any power saving mode during the recording e Recommended codec is Microsoft Video 1 e Use uncompressed video only for small recordings less than 2GB in file size DeltaPix 2006 VI Page 37 DeltaPix Camera Manual DpxView LE The AVI Recording Toolbar will appear when selecting the Video Capture Toolbar The File button allows you to save your video file By activating this button a new window will appear suggesting a default name as shown in the below figure Name of AY file to playfrecord Save in gt Documents and Settings FSR DeltaPixt ad Fol DeltaPix avi Fol DeltaPix3 avi GAl Users io Administrator A Mu Pictures E Documents IEAI Users WINDOWS CH Haissam El saach File name REES Save Cancel Save as type Video IT avi The filename is used for rerecording and playback Select folder and type the file name Finally save the file by pressing the Save button It is recommended to select Microso
22. option Selecting the All option will reset the camera as well as the software Note that all settings will be lost DeltaPix 2006 VI Page 17 DeltaPix Camera Manual DpxView LE 3 5 Settings Menu In the Settings Menu you can select various File View Image Camera Settings Help common settings 3 5 1 Options The options submenu includes the following Settinas Her options figure to the right Wen image captured gt bw Auto suggest Filename Prompt when renaming image Files First entry lists a number of possible events to be activated when a high resolution image is captured Play Sound when image saved or opened High Resolution Image Stamps Load Shading Correction file upon program start Selecting the Auto Suggest Filename entry BB Windows kf Warn when saving files with non persistent calibration information will enable the auto file name feature used TWAIN Data Source gt when saving files RT Reset Focus bar when Exposure changes Advanced High Resolution Settings Help Image Stamps enables you to add information to the image such as date time and text When image captured het uto suggest filename Kee Prompt when renaming image Files Magnification Play Sound when image saved or opened 2 Light source Frequency gt High Resolution Image Stamps Define Static Text Load Shading Correction file upon program start Ruler and Stamp Font E Win
23. the distance between two points of the original image To draw the ruler at an angle that is a multiple of 45 degrees hold down the Shift key while drawing These functions allow you to select the color font type and size L el Transparent t lz E DeltaPix 2007 VI 64 Page DpxView Pro Manual This is an example of some Be eae eee annotation and basic measurements on an image E EE EE EE RBS RBD GEO DR TE ME zem ua li Up a a aa Click on the object with the right mouse button to do the following B B e Undo the last user B action meae e Cut or copy the object KI kA f i d E me zen 17 to the clipboard e Delete the object e Bring the object to the front or send it to the back e Select all objects nae i f f RR C NM t gt U 7 e Change the line width and style properties e Change the foreground color Render Annotation el makes the annotations a permanent part of the image d Calculate Area and Perimeter are usually expressed in square centimeters and centimeters respectively Refer to the DoxView LE manual for calibration of images Settings Window Help a Magnification Calibrate Ctrl M feiss Microscope Interface Ctrltalkt Insert Calibration Bar in Images Mone Ruler and Stamp Font Windows M Embed DFI information in Image Rendered Das
24. the example located with the Button Tool paragraph Magnification menu 3 5 4 Light Source Frequency Set in accordance with the frequency File wew Image Camera Settings Help of the illumination DC 50Hz or 60 Hz S Options d Using 50 or 60Hz AC light may make the image flicker if the exposure time is not locked to the frequency Magnification Select the 50 or 60Hz option in this Ee Ee Be Case The best result is achieved if DC light is used This is due to the fact that most of the new manufactured microscopes have the DC light d e The auto exposure does not lock to the light frequency even though the right AC frequency has been selected e Flickering images caused by AC light may confuse the auto exposure and the auto white balance DeltaPix 2006 VI Page 22 DeltaPix Camera Manual DpxView LE 3 5 5 Windows This entry will display information on your File wew Image Camera Settings Help Windows operating system and the current CPU load as shown below Options gt Magnification d ae Light source Frequency Windows Windows iane HP Professional Edition j Windows XP Professional Edition Version 5 1 Service Fack Build 2600 Verzion 5 1 Service Pack Z Build 600 CPU ba CPU 954 Clase Piorrity Class Plornity Realtime O Realtime O High High Above Normal O Above Normal Normal Normal Below Normal gt Below Normal es Low TC Low
25. when image saved or opened High Resolution Image Stamps gt Load Shading Correction file upon program start ff Warn when saving files with non persistent calibration information TWAIN Data Source gt ff Reset Focus bar when Exposure changes Advanced Stay on Top Use Trayicon DirectX Support Calibrate Camera ki Save Profiles as ASCII White Balance algorithm A Enter License key Page 19 DeltaPix Camera Manual DpxView LE 3 5 2 1 DirectX Support Support for DirectX driver for integration to 3 part video ele D s rayicon application and software is included To enable the DirectX geg viel i driver select the Register option and disable DirectX with Ei Calibrate Camera gt r vw Save Profiles as ASCII Der GIS t er White Balance algorithm b When enabling the DirectX driver the below message is ReeSwr32 shown Press OK to continue When disabling the DirectX driver the below RegSvr32 message is shown Press OK to continue Li WI CllUnregisterServer in lutf ax succeeded 3 5 2 2 Calibrate Camera The micro scanning has been carefully calibrated from the factory Recalibration is not recommended in any case Contact your distributor before attempting to recalibrate Calibration requires a special calibration pattern P e as e n ot e th e Settings Help A i when image captured gt Calibr a ti on fu nction Ai SE M Auto suggest Filename H H ke Magnificati Promp
26. 6 4 Color Transform Halftone Select an image and then from the Color menu Color Post Acquire Process Window choose Transform Halftone move the slider to the right to S eg increase the halftone or to the left to decrease the halftone Invert Gray Scale Adjust 1 Transform m Remap d Color Resolution Histogram k Gray Scale Separate Colored Gray Paint Scaling Count Unigue Colors Please notice that it takes few seconds to view the window shown above when the Color gt Transform gt Halftone function is selected Color Resolution allows you to get the most from images by increasing the number of Bits per Pixel The choices are 1 to 64 bits The RGB color order is an additive color model in which red green and blue light are combined in various ways to create other colors Adding red to green yields yellow adding yellow to blue yields white In BGR color order adding blue to green yields Cyan and adding cyan to the red yields white Grayscale color converts image data to the Grayscale Colored Gray is used to convert a colored image into same gray scaled 79 DeltaPix 2007 VI Page DpxView Pro Manual 3 6 6 5 Color Remap Remap Intensity Graph is a function that allows some unique effects For every input value point along the x axis there is a unique output value determined by the height of the graph at that point In the graph s default state th
27. C Snapshot Settings Acquire snapshot 5 Snapshot Exposure 370 000ms The arrow button transfers Exposure Snaphot Gain 1 DUU Gain and binning preview information to 2 the snapshot window Appropriate values for snapshot exposure and gain Su Get oe pavers are suggested SC CO As preview vee Gi Qulality KOMPerSs ator iy Dm Speed Normal Sensor Clack Setting the sensor clock affects the noise level slightly Normal Sensor Clock is recommended The snapshot window makes it easy to set the snapshot parameters to acquire an image like in the preview but in maximum quality low gain for low noise and no binning for high resolution The arrow button also considers use of preview binning and adjusts exposure or gain accordingly It is recommended to use the Quality option while transferring parameters to the snapshot window Different parameters can be favored a As preview Transfers the preview parameters directly b Quality Adjusts exposure keeps gain low manually c Speed Adjusts gain adjust exposure manually Please note that binning cannot be used when acquiring snapshots The recommended way to use DeltaPix CCD cameras for fluorescence applications where it is possible to have both a high frame rate and a high exposure time for still image capture at the same time 1 Increase the gain to almost max from the Camera Settings Toolbar 2 If the im
28. DeltaPix Discover the Details 3 Technical Support If you have problems installing or using the software or with general camera operation you can e mail our technical support group at support deltapix com or you can contact you local dealer or distributor to get further direct support Please also visit our homepage at www deltapix com for software updates or other improvements for your DeltaPix camera 3 1 Sequence to install the DeltaPix software 6 Detach any DeltaPix camera and dongle for DoxView Pro from your PC Important Uninstall any already installed DeltaPix software use the Control Panel Add or Remove Programs Install the new package from your CD or from the DeltaPix web www deltapix com Attach dongle if any If it is the first time the dongle is attached you should see a Found new Hardware sequence Wait for the sequence to complete Later dongle attachments will issue the Windows ding dong sound Attach camera If it is the first time the camera is attached you should see a Found new Hardware sequence Wait for the sequence to complete Later camera attachments will issue the Windows ding dong sound Start DeltaPix software If you don t get an image possible solutions could be nO y g ge p Reset all Use menu Camera Reset All Touch the color gains due to an old software bug adjust the gains slightly 3 1 1 Dongle Check You can check that the dongle is properly
29. DeltaPix Camera amp Software Installation Manual DoxView LE amp DpxView Pro Infinity X DP 200 DP 252 DP 253 DP 300 and DP 450 Cameras DeltaPix Discover the Details 1 Preface Thanks on behalf of the DeltaPix team for using DeltaPix scientific digital cameras DeltaPix wants to carry out its service and with this preface provide an introduction to the user manual so that every one can gain the fullest possible advantage of it The manual consist of three parts Part one begins with a description of DeltaPix software installation manual that will guide you through the installation process Part two is the DpxView LE user and operational manual that invites you to learn how to manage and control DeltaPix cameras to the greatest advantage The manual will help you learning the flexibility and user friendliness of DeltaPix software Part three begins with hints to install DoxView Pro package and the dongle The second part of part three is the DpxView Pro user and operational manual Il DeltaPix Discover the Details 2 Installation Manual DeltaPix Scientific Cameras provide a quick and easy means of displaying high quality video and capturing high quality images on any USB 2 0 equipped desktop laptop or embedded computer Because they are USB based there is no need for a frame grabber Instead a single cable provides power full command and control and data transfer at soeeds of up to 24 MB s T
30. E blur to add to the visual impact an object has on the image x a Moise d Radial Blur Create the effect of spinning your image with the Radial Blur Sharpen R Zoom Blur Effect Radial blur rotates and blurs the image pixels around E gt EENG the selected center point See Distort j Anti Alias Create the effect of zooming into your image with the Zoom Edge e Average Blur that blurs the image along radial lines starting from the Spatial Filters V selected center point Force to Color d A Gaussian probability curve is the classic bell shaped curve Force Mono FS with a higher probability in the center of the curve and lower myama F probabilities to either side dd Open Images Add Thumb Images A Gaussian Blur effect takes each pixel in an RGB image and mixes it with adjacent pixels with Gaussian probability so that the pixel has great effect near its original location and less effect in a bell curve shaped way further away from its original location If a selection is present the Gaussian blur will ignore any unselected pixels Gaussian blur will also ignore invisible pixels Anti Alias Blur is used to smooth edges in an image and smooth jagged diagonal lines Average Blur is different to Gaussian Blur the smoothing effect is stronger and not completely independent of direction It looks more refracted 3 6 5 2 Effects Noise The noise effects dominate at low light levels For longer Ef
31. E 63 34 leie ei gl BINS ccs rca cece ee ea creme ces eee neem eee meee ek en cen eee men Aaa 63 35 Annotation ANG Measurements sisri aa aaa aaa 64 3 5 1 Heportmg PEEP 66 352 Measurements on Live Hreview 67 3 0 3 Image Recalibration ME 67 20 Man leet 67 3 6 1 SE 67 3 6 2 Eegeregie 69 POO E 69 3 6 4 Ire Secu steer E EAEE AE E EA E AE EE TEE E E A TE 71 3 6 5 SU a aster ces cs cnc se cette octane sect deg E E E ceed ge do eniesseteieceacaseansseeedes 73 She SE e 78 3 6 7 OSU Leef ee rel 82 3 6 8 APO EE 82 A PUIG GEIR MENU geet 83 5 DPXVIEW PRO EF EXTENDED DEPTH OF FOCUS MANUAL 00 ccssccsssecessseeesnseeeessenennees 84 6 DPXVIEW PRO EE EXTENDED EXPOSURE EXP MANUAL csscccssseeesseeesseeeenseeensees 86 7 DPXVIEW PRO AZ AUTOMATIC EXTENDED FOCUS amp EXTENDED EXPOSURE 88 7 41 DpxView Pro ZA Automatic Z CON te 88 DeltaPix 2007 VI DpxView Pro Manual 7 2 AZ COU ON a rece scence ic ce cates ces st wars enn ese a snc gene re aaa a aaa ai aaia i 89 7 3 DpxView Pro AZ Direct interface to Zeiss microscopes with USB polt cssscssseessseessseessees 90 7 3 1 Install the Microscope Driver e 90 7 3 2 Installing the Carl Zeiss MTB 2004 nnnnannnnnnnannnnnnnnnnannnnnnnnnnnnnnnnnnnnnnnnnnennnnnnnnnnnsrnnnnnnrennrensrnnrnnnennrennnn 90 7 3 3 Configuring the Carl Zeiss MTB 2004 92 0 ele cn G7 Ul kc anne mnetneetnne ane tenet e EE EE EEEE 94 or en RN L
32. E Deel Da rretan 99 La tafe H L DEE H latins The Viewfinder is the window which the image is viewed through to compose the scene 59 DeltaPix 2007 VI Page DpxView Pro Manual 3 2 7 Add or Edit Comments The Add or Edit Comments button makes it possible to add comments to an image Select this button and type the text inside the box as shown in the image Then save the image in order to save the changes have been done 3 2 8 Merge Function Ar Average Do one of the following to add images e Cum i 1 Open the images to be added 2 Select multiple thumbs by keeping the Ctrl button dowr The Merge function allows you to merge two images or more You can merge two images or more by taking either the average or the sum of them Right mouse click on the icon button will open the two possibilities There are several ways to do it The easiest ways are mentioned in the dialog box to the right 1 Open the images to be added one by one and then select the Merge button or 2 Select multiple thumbs by keeping the Ctrl button down and then select the Merge button Fluorescence Green Fluorescence Blue This is an example of two images that have been merged using the average function one time and the sum function another Wm E CIR ae Highlight Images rnana by Ctrl button Se S e Wed Preview live image viewer Fluorescence Red Merge using
33. E output window The region along with other low intensity regions is now black Note Can be undone with Ctrl Z DeltaPix 2006 VI Page 47 DeltaPix Camera Manual DpxView LE 9 Shortcuts Many functions can be activated by shortcuts 9 1 Camera Window Capture high resolution image Open high resolution image window Increase high res resolution Decrease high res resolution Save application profile Work Space Load application profile Work Space FastFocus mode on off Visualizer mode on off Full screen mode on off Loop through high res resolutions Toggle Black Frame Compensation on off Enable Auto Exposure to Converge Toggle Auto Exposure on off Fit to window Gain RGB adjust Help shortcuts Live Image Magnification Calibration Print Snapshot Questions and Answers Help Toggle Shading Correction on off Camera Settings Toolbar on off Place Spot White Balance Viewfinder on off Toggle Auto White Balance on off 9 2 Output Window Acquire high resolution image Open camera image window Make Mam Window the current window Save Image Open Image Print Image Ctrl C Show standard camera image Ctrl Z Undo Last operation Ctrl Alt Z Undo All revert to unprocessed image Alt Click Place annotation text Ctrl P DeltaPix 2006 VI Ctrl Alt A Color Correction Ctrl Alt B Capture Black Frame Ctr
34. NTOOLBAR EE 24 4 1 Configuring the button toolbar es sccicscnestncinsensienucSeveesienessnsiussnessncinuansesdeviuwcansenesanelvievecsteiesewsusbearusceswouss 24 A2 BOSE DA oien a E a EES 25 4 3 Calibration bar and magnification control sssssssuunennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnnm nnmnnn nnna 26 4 3 1 Configuring Magnifications ccccccccssscssecesseeeseeeeseeeeseeeceseeceseeceseeceaeeceaeeceaeeseaeecesaeessaeessaeeeeeeeseas 26 DeltaPix 2006 VI Page 2 DeltaPix Camera Manual DpxView LE 432 ele ODECE sssaaa E EAE aaa Eaa EE EA 28 wa FOCHS Ke EE 30 A Galtier Control BURONS sinsin a aSa NE aa E RANA 31 40 Usel Tu en 32 5 RICHT COCR MENU on 34 NEE 21 0 8 un e LE 34 RS OEM a E E E OT E OA 34 5 3 Sensor a C6 E 34 5 4 EO TR 35 55 Wh hit Ba lance Sil INN ic sass esis cscs ca cesc ccm deca seenwtsntestees cece cesteutensataniadieua aaa aaa aaa 35 5 6 Black Frame Compensation Submenu ccsssssscssssssssesssessseessseesseesensceenseeenseeenseeenseeenseeeneeeens 36 5 7 Shading Correction EE 36 5 8 Camera Settings Toolbar Visible s scsssssssssssssssesseeesseessnseesnseesnsessnseesneesnseesnseesneeeseeeess 37 5 9 Video Capture Toolbar Visible Recording AVI s scssssssssssseesseeesseeesseeeenseeenseeenseeensenensenens 37 S10 Main MENU aaa E AAEE E A AAAA A AEAEE 39 S11 B ton Le ln EE 39 5 12 AUTO HIGE Bitten Toolbar sissa EAEE E EAE 39 S13 Wo
35. age Viewer ezDICOM Ctrl Alt F Focus Aid tool restart Ctrl Alt S Capture Shading Correction Frame Ctrl Alt T Toggle TWAIN Data Source on off Cirl Alt X Exit Program Ins Increase Exposure Del Decrease Exposure Carlines Fast Increase Exposure Ctrl Del Fast Decrease Exposure Ctrl F1 Histogram and Profile 94 DeltaPix 2007 VI Page
36. age from the attached DeltaPix Camera Resolution and zoom pan can be selected via the menus Status bar Miscellaneous status information is shown in this bar Can be enabled or disabled from e g the View menu m 1280 x 1024 Ho DS i 15 fps SE Connected CO 08 46 24 30 11 2006 At the top of the window you will see the Main Menu bar Below the Main Menu a configurable button bar and in the bottom a status bar DeltaPix 2006 VI Page 6 DeltaPix Camera Manual DpxView LE 3 Main Menu The Main Menu figure to the right has the File Vie Image Camera Settings Help following functions File View Image Camera Settings and Help 3 1 File Menu Several functions are available in the File Menu File view Image Camera Settings Help Save Snapshot Print Snapshot Workspace and Exit Lt save tnage functions itr Print Image Ctrl F Ee En co Image to Clipboard Chic a _ Workspace B Jave SS Open Chri alk s 3 1 1 Save Snapshot The Save Snapshot function will grab a snapshot of the live video image shown on the screen and open the Save As window figure to the right Save Snapshot as Save in O Snapshots D e DI rt PS Select the folder and type the file name you wish to assign to the snapshot The program will automatically suggest the most recent filename used if this feature is enabled in the Settings menu File name Test Save as type Windows Bitmap
37. age is not bright enough yet select binning x2 from Camera Sensor Region menu Decrease gain or exposure if the image gets too bright Remember to do a spot white balance 3 Select snapshot settings from Camera Snap Shot Settings menu 4 Inthe snapshot settings menu set the gain to 2 5 5 Inthe snapshot settings menu click on the calculate button Now it is possible to have both a live image with high frame rate and at the same time to capture snapshots with long exposure times several seconds and low noise Notice that the live image will have a high noise level but the snapshot will have low noise because of the long exposure time Every time the exposure time or gain is changed in the live image the calculate button in the snapshot settings menu must be changed in order to capture still images with the same brightness level as the live image DeltaPix 2006 VI Page 12 DeltaPix Camera Manual DpxView LE 3 4 5 Black Frame Compensation There are two purposes for the Black Frame Compensation function SCH Capture Ctri Alt B Ho Black Frame Compensation Enable ctrl E When extended exposure times and high gain settings are used some of the pixels will show static noise spots resulting in blue green or red pixels being permanently turned on so called Hot Spots Hot spots are usually a result of leakage current in the physical sensor pixels The problem with pixel leakage is tha
38. agement Software This gives you the possibility to use both software versions When the DpxView Pro is started the first time after the program has been installed your screen should look like this Window Header Can be enabled or disabled from e g the View menu Configurable Toolbar Buttons and tools can be individually enabled or disabled This allows the user define a customized view Splitter Drag Left Right DpxView Pro Main Menu Can be enabled or disabled from e g the View menu Klee maer Mansgrmgnt BE ce ed ts OR EE fein E ue malg e Epone ie a BB es E J i LR Annotation and Measurements L a DpxView Pro Button menu x E P aa JE DpxView Pro Image Archive PE F Leali Image Field Displays live image from the attached DeltaPix Camera Resolution and zoom pan can be selected via the menus d ei S li m KE T i Da n i oO CH Splitter that can be dragged Left Right to make thumb area smaller or larger Status Bar Miscellaneous status information is shown in this bar Can be enabled or disabled from e g the View menu LA A ei d fis Ce il j al i ER PO E ZG V y E Hints 128 La Demosaie D Ie 100 D ATRIA E Sie etd Ge jaag 55 DeltaPix 2007 VI Page DpxView Pro Manual At th
39. amera Add Date Time DeltaPix Duration Mm Sec ES GES is Playback ZU Ips Is Ire Si om gt Codec Quality 804 Microsoft Video 1 v S wie Uncompressed H R The Black Frame Compensation Button helps you to eliminate the static noise from the image DeltaPix 2006 VI Page 32 DeltaPix Camera Manual DpxView LE The Shading Correction Button makes the image look nicer by making the illumination more uniform The Optic Calibration helps to superimpose a calibration bar in the image This button enables you to save a Snap Shot This button enables you to print a Snap Shot This button will open the installation and the user manual of the DeltaPix software of This button enables you to send mails directly from DeltaPix viewer to DeltaPix support team This button closes the DeltaPix viewer The Image Histogram displays the e number of pixels at each grayscale EE value within an image Image Histogram A klp Ie Logarithmic view O 20 40 BO 80 100 120 140 160180 200 220 240 This function supports camera with active cooling DeltaPix 2006 VI Page 33 DeltaPix Camera Manual DpxView LE 5 Right Click Menu Right clicking on the mouse while positioning the mouse cursor anywhere on the preview image will activate the right click menu shown in the figure to the right 5 1 Grab Image This function helps you to capture a hi
40. ant Red Flour Low bmp S insect bmp Filename Plant Dark Low bmp Fies of ype Windows Bimap bmo S C Show Options on Open Preview Page 63 DeltaPix 2007 VI Page OS DpxView Pro Manual The Save button a allows you to archive captured images l Sa Gi Other functions are also available like the Copy 2 Paste m and Print Preview button This button el ls allows you to zoom in the live image and or the still image to 100 This function sets the live image and or the still image to fit to window Snap Function resizes the window to fit to an image takes you back to the live preview window 3 5 Annotation and Measurements The Annotation tools are shown in the figure below When the button toggles annotation is active Ie beee a EE ltere x Simply click on the appropriate icon and draw right on the image to AIN seol produce overlays of circles arrows and more The annotation tools allow you to easily highlight features or mark up an image Add text to an image in three simple ways Text Pointer Rich Text and Normal Text C d Peri ither by Ellipse Pol SS eee m sa ee geste the Wie Leer re ie Aik directly on your image with this button 2 Measure the length of an object either by the Ruler or Polyruler functions The Ruler Polyruler annotation is a line polyline that can be used to measure
41. ation combo boxes Proceed opening file Wes i D a mi _ E optics path Notesblok BX Fier Rediger Formater wis Hj lp Frimostar EE em E Optics Pathi Configuration For each microscope type you can change the default created 21706 2005 magnifications Double click on the second optic Se This file optics pathh txt magnification will open a message box Select Yes to Edit with care open the ASCII file ua Klek E optics path Notesblok gt N1TR On gt gt Primoastar Filer Rediger Formater wis Hj lp No empty lines after this line Za ff Optics Pathe Configuration ff Created 21706 2005 ff This Tile optics path2 txt f f Edit with care ie ME DpxView Pro i Optics definition files should be edited with great care Jj To make the changes take effect the program needs to be restarted ft No empty lines after this line Proceed opening File Wes No Type the new magnification values Then select File Save Finally close the ASCII file To make the Beles tet changes to take effects restart DeltaPix application Mikon ae Optics x 4 7 The red color indicates that the objective has not Nikon di been calibrated yet 4 10 40 100 To calibrate an objective in this example a 4x objective select the Settings Magnifications Magnification Calibrate or Ctrl M option Position the cursor at the left end of a horizontal or vertical object exactly in the same wa
42. ations of PCs especially laptops and DeltaPix cameras may require an external power supply e We always recommend use of external power supply for all CCD cameras e If your Infinity X produces poor images this may also indicate that external power is needed The following messages are also indicators of need for external power Power Surge On Hub Port Power Surge On Hub Port The Infinity s USB 2 0 Camera has exceeded the power limits of its hub port For assistance in solving this problem click this message The Infinitis USB 2 0 Camera has malfunctioned and evceeded the power limite of its hub pork ou should disconnect the device The location of the device is shown in bold type gz USB Root Hub 6 ports Unused Port Unused Part 35 Infinity USB 2 0 Camera Unused Part Unused Port Unused Port Recommendation To reenable the port disconnect the hardware and click Reset If you click Close the port will no longer function VI DeltaPix Discover the Details 4 Using the viewer software After double clicking on the DeltaPix viewer icon the viewer application starts and if a camera is attached you should immediately be able to see live video from the camera on your screen An on line help manual is available through the help menu VII DeltaPix Discover the Details DeltaPix Camera Software User and Operational Manual DoxView LE 1 11 11
43. because you can select a lower exposure time and thereby a higher frame rate which makes adjusting of the object and focusing of the microscope or lens easier Binning will not work together with high resolution snapshots DeltaPix 2006 VI Page 15 DeltaPix Camera Manual DpxView LE 3 4 9 Zoom Zoom selection enables you to directly select different levels of Zoom 100 200 400 a00 G 1600 a200 The zoom can also be set using the mouse wheel Eit to Window Ctrl F 3 4 10 Frame Rate Mode This mode is only available with CMOS cameras The default setting is Adaptive to 100 as shown in Framerate mode Manual the figure to the right t Adaptive to 50 Seek E E ene e Adaptive to 100 In order to reduce the CPU load to 50 select the Adaptive to 50 option e Manual means that the program just selects a default frame rate and doesn t change that unless the exposure time increases beyond the maximum allowed It also enables the user to change frame rate manually e Adaptive to 50 will try to set the frame rate at a high level even if the exposure time is adjusted to a higher level than the current frame rate allow for e Adaptive to 100 will provide you with the highest possible frame rate at the selected exposure time Please notice this selection will assign almost all of the CPU capacity to the video stream If sufficient lighting is provided and the computer has sufficient CPU capacity the video d
44. bmp v Cancel Windows Bitmap bmp JPEG jpa Finally in the Save as type drop down menu select the appropriate file format Note The JPEG 2000 format will result in a superior image quality compared to standard JPEG However when saving snapshots in JPEG 2000 format a certain delay must be expected due to an increase in CPU load especially when using a computer with limited performance 3 1 2 Print Snapshot Print Snapshot will open the Camera Print Image window Ops view Print Image allowing you to adjust various settings prior to print the Layout snapshot C Auto page ni E v Center on pagels al x Scale to page SE Page numbers Portrait Include title Landscape Title DeltaPix 2006 VI Page 7 DeltaPix Camera Manual DpxView LE 3 1 3 Work Space The Work Space function iS a system SE Aust ucalo E Save Application Profile as setup for your personal applications Kl ee abs e Si Profile DPX a Dubai emo2 DPX IS Scientific DPX E Abdulhameed DPX E Standard DPX Sent DPX Si Pathology DPX E PathologyProfile DPX S DubaiD emol DPX Contrast 1 364 It enables you to save your preferred Brightness 0 662 camera settings into a profile file for later retrieval and use with a similar Som application Hue 4 172 Saturation 1 232 File name HESESESRSC Save as type Profile dpx we Several profiles matc
45. bs Directory and Options as shown below Thumbs Directory Options 3 3 1 Thumbs Thumbs as shown previously allows you to view images as Thumbnails 3 3 2 Directory The Directory enables you to view the archived images in a directory as shown below 62 DeltaPix 2007 VI Page DpxView Pro Manual 3 3 3 Options The feature Options helps SS SSS SSS ee you change _ the Se appearance of the thumbs se 5 For example you can select the back ground of the thumb by selecting your favorite color Please background color of the thumbs is turned into cyan Ga blue as shown below e Select the image format to be viewed in the image archive Thumbs File Mask to sort out files will display all files E g 1234 will show all files beginning with 1234 Selected Thumbs Color will open the color palette notice that the 8 geg eis a A Po LORE ls ell eee PI biseblzlsl stzTsl i ai L lz L ee eo Hie 3 4 Shortcut Buttons ete Re LB Preview Gm Activating the Open Button Se will open a new Look in Gp Images psView ro Jo e e Ele window where you can select an image from your kaf N Cancer Low bmp ar ch d e History N Mouse Phase Contrast Low bmp i N Plant Blue Flour Low bmp IS Plant Normal Low bmp Press the button open to browse an image DEEN E Plant Phase Contrast LOw bmp Kl Pl
46. can select the active sensor region 640 x 460 B00 x 600 1024 x 766 1280x1024 1260 x 1024 x2 subsampled The selected region is always centered on the mid point of the sensor leaving the outer parts unused Reducing the sensor area will allow for higher frames rates and smaller file sizes 3 4 1 1 Sub Sampling File View Image Camera Settings Help Ge sensor region is 1260 x 1024 FE Black Frame Compensation Shading Correction Frame Average Software Binning Q Framerate mode LJ Zoom FastFocus Mode et Visualizer Mode Preview Demosaicing Color Correction Flip Image d Reset Chrl alt 4 640 x 480 800 x 600 1024 x 765 1260 x 1024 1280 x 1024 x subsampled Selection the 1280 x 1024 x2 sub sampled uses every second pixel thus allowing display from the entire sensor but at a lower resolution Frame rates and images file sizes corresponds to the 640 x 480 selection The advantage of the sub sampling is the large field of view with high frame rate the resolution is however somewhat reduced Examples e With sensor region size 1280 x 1024 frame rate can be 15fps only limited by the USB bandwidth assuming exposure time less the 1 15s the captured image file size is 3 75 MB 3750 KB e With sensor region size 1280 x 1024 2x sub sampled the frame rate can be 51 fps again limited by the USB bandwidth and now assuming exposure time less the 1 51s
47. centage 131 DeltaPix 2007 VI Color Post Acquire Process Invert Gray Scale d Adjust j Transform d Remap i Histogram d Separate d Paint Scaling i Count Unique Colors Color Post Acquire Process Window Invert a Gray Scale 8 Bit Adjust NEE Transform d L 16 Bit Remap Histogram k Separate Faint Scaling j Count Unigue Colors Color Post Acquire Process Window Invert Gray Scale Adjust Transform Remap Histogram Separate Paint Scaling Count Unique Colors J Brightness d Contrast Hue Saturation d Gamma d Balance Colors Contrast Hue Saturation and Gamma can also be adjusted in the same way as the brightness 18 Page DpxView Pro Manual Balance Colors refers to the balance of red green and Balance Colors blue in your images In common usage balancing colors means creating natural looking images without color casts an overall imbalance of colors in an image Changing the Overall color balance use the Red Green Blue command to adjust the amount of red green and blue in your image to make color corrections by changing the overall color cast Before Red Weights Red 19 Green Di CG Blue Green Weights Red 37 Green 21 Blue 15 Le a Blue Weights Red 3 k Green G Blue SS Idle Co 3 6
48. ction file upon program start wi Warn when saving files with non persistent calibration information TWAIN Data Source bw Reset Focus bar when Exposure changes Advanced gt E H H R Enable Ctrl Alt T Acquire automatic Open and close silently Run minimized Use swap file This may cause a long delay e g if the exposure time is set to maximum and the resolution is set to 21 Mega Pixels Disabling the Acquire automatic function will allow the user of the third party application to adjust the camera settings before the capture process is started Open and close silently will run the DeltaPix camera software in the background when the Twain interface is activated in a third party program Run minimized will run the DeltaPix camera software minimized but you will always be able to maximize it for live video display or taking advantage of the advanced features not available through the standard Twain interface 3 5 2 Advanced Stay on top will always leave the DeltaPix camera ons application in front of your CR windows screen Q Use tray icon will install a small D ss DeltaPix tray icon in the lower right corner of your Windows system It is possible to start the capture process by right clicking the icon DeltaPix 2006 VI Magnification Light source Frequency gt When image captured gt hf Auto suggest filename gt Prompt when renaming image files Play Sound
49. d Note It may be necessary to configure or disable internet security to make the MTB server work d Note When the microscope window has focus then it is possible to use the mouse wheel to move the Z axis in small steps 7 3 1 Install the Microscope Driver The microscope drivers will be installed when connecting the microscope to the PC USB port The Carl Zeiss driver CD may be required The installation will run automatically and may last a few minutes 7 3 2 Installing the Carl Zeiss MTB 2004 In the MTB 2004 directory run the setup Choose Setup Language 90 DeltaPix 2007 VI Page DpxView Pro Manual ie Carl Zeiss MIB 2004 InstallShield Wizard Welcome to the InstallShield Wizard for Carl Zeiss MTB 2004 The InstallShield R Wizard will install Carl Zeiss MTB 2004 on your computer To continue click Next WARNING This program is protected by copyright law and international treaties ie Carl Zeiss MIB 2004 InstallShield Wizard Customer Information Please enter your information het ie Carl Zeiss MIB 2004 InstallShield Wizard Setup Type Choose the setup type that best suits your needs Please select a setup type Complete All program Features will be installed Requires the most disk space O Custom Choose which program features you want installed and where they will be installed Recommended for advanced users Install MTB Server as Service InstallShield In
50. d Optics x 4 Nikon 2 Non MH Please note that the red colour is turned to green colour which indicates that the objective is calibrated d Optics x 10 Please note that the other objectives are turned to yellow colour which indicates that the objectives are calibrated but in interpolating way To calibrate the objective 10x repeat the mentioned process above select Settings Magnifications Magnification Calibrate option Position the cursor at the left end of a horizontal or vertical object exactly in the same way as described in the previous example Enter the Ruler Length and the Unit in the two fields representing the known distance in the image When the warning dialog box starts up select No to keep the calibration of the Optics x 10 magnification 4x and to calibrate the objective 10x individually Mikon E h 4 4 Focus Tool The Focus Bar is a helpful tool to indicate if the object is in focus or not Itis Focus SE necessary to move the object to the right and to the left from the focus point to aS establish a reference IRRRRRRRRRRRRRRR Restart button will reset the focus bar to the maximum focus point The focus bar will also reset if you alter the value for the Exposure Time using the exposure bar DeltaPix 2006 VI Page 30 DeltaPix Camera Manual DpxView LE 4 5 Camera Control Buttons This button allows you to select the desired resolution vi The first mode 1280 x 1024 Demosaic
51. dit View Image Effects Color Post Acquire Process and Window Fle Edit view Image Effects Color Post Acquire Process Window 3 6 1 File The functions available in the File Menu are listed in the figure to the right 3 6 1 1 Open and Save Functions Open Image feature allows you to open saved images from your archive in various file formats Save File as allows you to save images in various file formats like JPEG BMP and TIF Convert File enables you to convert the image from one format to other and the Close option will close the image window DeltaPix 2007 VI File Edit wiew Image Effects Open Chrl c Save s Chril 5 Convert File Close Color Merge Enable Drop File Print Ctrl Alt P Print Preview Import from d Export as d Capture Screen Cbrl Alk 5 Set as Desktop Wallpaper Image Info Exit 67 Page DpxView Pro Manual 3 6 1 2 Color Merge Color Merge enables you to regulate the color space CMY and CMYK Color Spaces are often used in color printing A CMY color space uses cyan magenta and yellow CMY as its primary colors Red green and blue are the secondary colors K the black color is used to darken the colors created by the other three process colors HSV Hue Saturation Value and HLS Hue Lightness Saturation are color wheels The hue is what is normally thought of as color Saturation is the amount of
52. dow F2 between viewing the DeltaVu Still Image or a direct snapshot from the pO Sieve Camera SCH Camera Still Image Chr DICOM Data This allow for a direct comparison between the DeltaVu image and the Original image DeltaPix 2006 VI Page 44 DeltaPix Camera Manual DpxView LE ezDICOM is a small free viewer find it at www mricro com Dovie DICOM Data DicomWorks is a comprehensive and free viewer Find it at Niels Munk www dicomworks com 1570 Silly E n DICOM Images comprises tags for e g patient and comments Patient Comments L00ks Healthy The following tags can be directly attached to DICOM images Institution Skejby University Hospital from the DeltaPix Viewer Address KRANKEN Referring Physician Frofessor Dr Gormseon Performing Physician Professor Dr Gormseon Study Description Brian Study Diagnoses Description LA Study Id 1570 123 Study Comments None None Series Description Bright Field Microscopy Needs More S tudd Image Comments Comments OT Other YL Visible Light GM Microscopy XC Macro 8 3 Zoom Menu The zoom menu allows you to select File View Zoom Effects Batch Process Undo Camera Window F2 between different levels of zoom Also the 100 mouse scroll wheel may be used for the ae zoom function however it may be needed Few to select the zoom function manually first t00 S00 if the scroll wheel previousl
53. dows ket warn when saving Files with non persistent calibration information TWAIN Data Source ki 4dd Static Text to Images Add Date stamp to Images wi Reset Focus bar when Exposure changes J E Add Time stamp to Images Advanced ki The Define Static Text function opens a new window StaticText where you can type the default text you would like to DetsP Discover the ect superimpose on all images The Font function opens a new window where you can select the font of the text Font Font style 5 Regular E q ize 7 Cancel 12 14 O Palatino Linotype 18 Papyrus 24 O Perpetua Effects F Strikeout a Underline anne Color Red v Script Westem v DeltaPix 2006 VI Page 18 DeltaPix Camera Manual DpxView LE The resulting image can be seen in the figure to the right 3 5 1 1 Twain Data Source The Twain Interface is default enabled in the Settings menu In many programs with Twain access an Acquire function is available If the Acquire automatic function is selected activating the Acquire function in the third party application program will initiate the image capture process Settings i Help o Option Y Light source frequency gt BB Windows When image captured gt kW Auto suggest filename me a Prompt when renaming image files Magnification gt Play Sound when image saved or opened High Resolution Image Stamps gt Load Shading Corre
54. e DpxView Pro Manual Below is an illustration of the Extended Exposure and Extended Focus that guides you to how to manage these two functions Relative Exposuretinie O Bio 8 50 IL Jw CH e lt lt OR 150 ee i oie teil lt lt 8 RC FOC G2 EXP dck Vertical Button Bar DeltaPix 2007 VI x Close all Sources Auto close Sources a Extended Focus X Radius 10 l 29 Create Ki 11111 III III ey Deformation 10 wE IIIIIIIIIIIIIIIIIIIIIIIIIIIIII x Close all Sources Rotation 10de z Auto close Sources Smoothing 4 de Default d Magnification 10 _ Allow Resampling P Show Hints Automatic Extended Focus Enable automatic control ais Device Standa BSMC1 se No Z axis device Zeiss MTB standa ASME i he Gi viended Exposure MA CHE MC Neutral Pos Power Save Swap Up Dn Show Hints 900 Steps s Calibrate 0 001rmm Logor Standa Controller Interface Slices c Horisontal Button Bar E Mo Device e feiss MTB KR Standa 65hMC1 oo C E v Auto Logon Reference MC Object to Eye Object to Camera ow Enable Z Axis Control Zeiss Interface C C Mouse click RC Right Mouse button click MC Middle Mouse Button Wheel Click Page DpxView Pro Manual 8 Shortcuts Many functions can be activated by shortcuts Camera W
55. e E 94 oe UID VV e E 94 52 DeltaPix 2007 VI Page DpxView Pro Manual 2 Sequence to install the DeltaPix software 1 Detach any DeltaPix camera and dongle from your PC 2 Important Uninstall any DeltaPix software already installed use the Control Panel Add or Remove Programs 3 Install the new package from your CD or from the DeltaPix web 4 Attach dongle if any If it is the first time the dongle is attached you should see a Found new Hardware sequence Wait for the sequence to complete Later dongle attachments will issue the Windows ding dong sound 5 Attach camera If it is the first time the camera is attached you should see a Found new Hardware sequence Wait for the sequence to complete Later camera attachments will issue the Windows ding dong sound 6 Start DeltaPix software If you don t get an image possible solutions could be e Reset all Use menu Camera Reset All e Touch the color gains due to an old software bug Adjust the gains slightly 2 1 Dongle Check You can check that the dongle is properly installed see EAO the device manager where the USB Dongle should S Fie Acton view window Hela appear Om S 2 a Computer Management Local a VD Pibus CH System Tools E Processors Di Event Viewer Sound video and game controllers 4 Shared Folders System devices 2 Local Users and Groups E Universal Serial Bus controllers Perfor
56. e function is a straight line from bottom left to top right In this case each input value produces an equivalent output value and the graph has no effect The graph is a function that lets you remap intensity values the Value component in the HSV Color space into other intensity values 3 6 6 6 Color Histogram Histogram provides information about the image concerning the intensity or gray level distribution of pixels This information can be used for many image corrections for the proper display of the image Histogram Equalization linearizes the number of pixels in an image based on the specified RGB YUV or GRAY color space This can be used to bring out the detail in dark areas of an image A Histogram Equalize will almost always lose some image information when the pixels are remapped to the new brightness values Despite the loss of the information the result can be quite pleasing Color Post Acquire Process window Invert Gray Scale Adjust Transform Remap Intensity Histogram P Hue Separate Paint Scaling Count Unigue Colors Color Post Acquire Process Window Invert Gray Scale Adjust Transform Remap a Histogram mae Separate Stretch Intensity Paint Scaling contrast Count Unigue Colors Stretch Intensity increases the contrast in an image by centering maximizing and proportioning the range of intensity values Unlike ordinary contras
57. e the sliders Digital Gain Red 102 Green 1 000 Blue 1 014 Refresh this Form 5 6 Black Frame Compensation Submenu There are two purposes for the Black Frame Compensation function ES Capture FAHI Black Frame Compensation Enable When extended exposure times and high gain settings are used some of the pixels will show static noise spots resulting in blue green or red pixels being permanently turned on so called Hot Spots The way to eliminate hot spots is by acquiring a black frame This frame can be used as base for compensation By placing the camera sensor in complete darkness they may be eliminated so nothing but the Hot Spots are displayed and select the Capture function When the reference image has been captured lights may be turned on again finally when selecting the Enable entry the static noise is eliminated from the image After a while you may have to repeat the procedure 5 7 Shading Correction Shading Correction is a powerful way to process the image for quantitative intensity measurements B Capture ER Shading Correction SC Enable wl Shade Correct Live Image Open Save The method by which images are produced the interaction between objects in real space the illumination and the camera frequently leads to situations where the image exhibits significant shading across the field of view In some cases the image might be bright in the center and decrease
58. e top of the window you will see the Main Menu bar Below the Main Menu a configurable button bar and in the bottom a status bar Fie iew Image Camera Settings Window Hel The Main Menu in the live preview sao Se atk P and the Exposure 22ms Focus E Met Restart B H JS EXP WB Git H 1 llnnnnnnnnnnnnnnnl eo SNI e Configurable button bar 2 are not be described in this manual You can refer to the manual of the standard package DpxView LE for a detailed description of these DpxView Pro has the advantage of being able to view the live image preview with the captured still images at the same window 3 2 DpxView Pro Button Menu Bar File Open Image button Save Image button Change Image Directory button The Delete button allows you to delete an image from the thumbs Ctrl Click Acquire The Capture button allows you to capture an image from the camera View The Magnifier button lets you to Zoom with a view The Viewfinder is the window where the image is viewed through to compose the scene Add or Edit Comments button makes it possible to add comments to the image Process The Merge function allows you to merge two images or more either by the Sum or Average of the images S The Intensity function allows you to change the brightness of the image change the contrast of the image and get some unique effects Hi
59. ect dialog to distort the active image using a wave radiating from the specified center Looks like ripples in a pond Swir spins the image according to the rotation angle to produce a swirl pattern Wave distorts the bitmap with two perpendicular Before After waves in any specified direction or shape a mmm Free hand Shear shears the bitmap by the wave Amplitude EE gt Zoom Factor 5 5 d rawn Exposed Area Pivot Use Color iS a x io B Y WS Cursor Pos Iout SY 3 6 5 6 Effects Edge Detect detects the edges in an image and their strength Effects Color Post Acquire Process wind D D D D D D Blur S The Erosion filter erodes objects at their boundaries making Ge them appear thinner j Sharpen The Dilation filter makes an object thicker SEELEN d Distort d le Edge Detect Spatial Filters b Erosion Force to Color 3 Hiabin id Force Mono FS Invert Image Fe 4dd Open Images dd Thumb Images 3 6 5 7 Effects Spatial Filters Effects Color Post Acquire Process Window Spatial filters can be pre defined or user defined and T ES _ include gradient laplacian sobel prewitt shift and S l difference line segment Noise Sharpen Spatial Filters can be used for line detection point Calculations i detection or to smooth high spatial frequencies low pass Bee filter D Edge d Use one of several predefined spatial filters Spatialriter
60. eg joeg2000 DICOM and TIFF It allows complete camera control settings as gain exposure time white balance color correction and many more Furthermore AVI recording simple text annotations focus tool zoom and pan and individual user profiles The user interface can be configured to the user s individual need allowing simple and intuitive operation Advanced functions such as Dark Frame Compensation Spot White Balance Auto White Balance Converging Auto Exposure Pixel Binning Frame Averaging Microscope Optics Calibration and Shading Correction TWAIN driver for interface to 3rd party programs and DirectX support is also available The software and updates for the software can be downloaded directly from our homepage Go to http www deltapix dk downloads _software htm for more information DeltaPix 2006 VI Page 5 DeltaPix Camera Manual DpxView LE 2 Initial View When the DeltaPix viewer is started the first time after the program has been installed your screen looks like this Windows Header Can be enabled or disabled from e g the View menu Main menu Can be enabled or disabled from e g the View menu File View Image Camera Settings Help Exposure 50ms a Focus Festal CO ze Iennnnnnnnnn SC Configurable Toolbar Buttons and tools can be individually enabled or disabled This allows the user define a personal view Image Field Displays live im
61. enser 0 9 H D Ph DIC Motorized Foto Tube with Shutter Eyepiece 10x Eyepiece SF25 bad Cameraport No Camera Adapter K 2x Motorized Sideport Changer Button settings Automatic functions X No Fluorescence Lamp No ApoTome No Stage Name z No Camera Adapter S 0 4 Camera Adapter WS 0 5 Camera Adapter eg WS 0 63 Camera Adapter Ls pe tx Camera Adapter S 1 6 Camera Adapter Kei 2 5 Camera Adapter S DigiCam Adapter d30 S Digicam Adapter T44 Cameraport No Camera Adapter Cancel IE MTBConfig File Options E B NewConfigurationt a Axio Imager Z1 2 6x Motorized Nosepiece a E 10 Motorized Reflector Changer Motorized Focus D No Tubelens Turret H Reflected Light Path Transmitted Light Path Motorized Condenser 0 9 H D Ph DIC Motorized Foto Tube with Shutter Eyepiece 10x Eyepiece F25 Cameraport 1x Camera Adapter S 2x Motorized Sideport Changer Button settings Automatic functions X No Fluorescence Lamp No ApoTome No Stage Name bad No Camera Adapter Kei 0 4 Camera Adapter WS DU Du Camera Adapter Kei 0 63x Camera Adapter 7 1x Camera Adapter S 1 6 Camera Adapter 7 2 5x Camera Adapter S DigiCam Adapter d30 Y DigiCam Adapter T44 Cameraport 1x Camera Adapter d Note It may be necessary to configure or disable internet security to make the Zeiss MTB 2004 server work DeltaPix 2007 VI 92 Pag
62. f the stepper motor when it is not moving Normally there is no need to use this option The Start up Calibrate and logon tipping these two options will make the system automatically log on to the Z stage and calibrate it when the software is started 89 DeltaPix 2007 VI Page DpxView Pro Manual 7 3 DpxView Pro AZ Direct interface to Zeiss microscopes with USB port The DpxView Pro can retrieve magnification information and control the Z axis of Zeiss microscopes supporting the MTB 2004 If connected to an intelligent Zeiss microscope then use menu Settings Magnification Zeiss Microscope Interface Ctrl Alt Z to get the logon menu Radiue 10 a d Deformation 10 OG Close all Sources Auto clase Sources o 4 se Default Magnification 10 Allow Resampling i e Show Hints Microscope Logon ne Extended Focus Automatic contral Bidirectional J coon Z as Device Zeiss MTB A D Auto Logon Reference Configuration Object to Eye Slices Object to Camera SE E fal E L Enable Z Axis Control Extended Exposure Publish DOF a Enable eut Lee Rotation 10deg SteREO Lumar Y 12 m ea Auto Login eme Gei Eyepiece Cameraport Magnification 20 4 Dialog for microscope logon Dialog for a logged on microscope It is not really important which one of the Eyepiece or Camera Port magnifications is used as reference
63. f this is your first installation or you have uninstalled previous versions of hard and software the Window s New Hardware Wizard will pop up detecting a new Unconfigured Device Select Install the software automatically from the options that are presented to you and click Next A warning may appear notifying you that the drivers have not been digitally signed by Microsoft Click Continue Anyway to continue with the driver installation Then click Finish to complete the installation of the drivers 8 After a few seconds the Window s New Hardware Wizard will pop up again if it doesn t disconnect and reconnect the camera to the computer detecting an Infinity X USB 2 0 Camera device Select Install the software automatically from the options that are presented to you and click Next A warning may appear notifying you that the drivers have not been digitally signed by Microsoft Click Continue Anyway to continue with the driver installation Then click Finish to complete the installation of the drivers 9 You should now be able to run the application software called DeltaPix camera from your Start menu and enjoy Note Windows will require you to re run steps 8 amp 9 each time you plug the camera into a new USB 2 0 port You must have administrator privileges the first time the camera is used on any given USB 2 0 port You may wish to repeat the installation steps at this time for all USB 2 0 ports on your computer IV
64. fects Color Post Acquire Process Window exposures than one second noise effects can dominate Blur 20 Use the Median Filter to remove or minimize noise Nose Median Sharpen l Add Moise Noise is any random discrepancy in an image If your image LEE gt Max looks like it has static or it has dots or flecks use the median filter to smooth it out Distort Min Edge t You control the effect by specifying the size of the Spatial Filters b neighborhood that is used for calculating the median value This filter also tends to preserve edges Specify larger sample SE f sizes to increase the effect Force Mono F5 Invert Image Fe 4dd Open Images Add Thumb Images You may at times need to Add Noise to an image For example if you are creating a composite image using one image with little noise and another image with more noise you can add noise to the first image to make them match 13 DeltaPix 2007 VI Page DpxView Pro Manual Or if you have blurred or painted a portion of an image that contains noise you may need to add some noise back into the area that you have modified Noise can also be used to add texture to an image Use the Max dialog to erode or shrink dark objects It makes dark lines on a light background thinner Use the Max filter to un blob the dark lines 3 6 5 3 Effects Sharpen The standard tool of choice for sharpening is the Un sharp Effects Color Post Acquire Process
65. fects gt Calculations gt Add Bitmaps function is selected 74 DeltaPix 2007 VI Page DpxView Pro Manual 3 6 5 5 Effects Distort Use the Freehand Wave filter to distort the active image using the wave drawn on the Curve Control and the rotation angle selected Use the Wind effect to create thin lines directed along a certain angle on the specified bitmap Use the Polar Effect dialog to convert the active image from rectangular to polar coordinates and vice versa Take your image and turn it end to end It s a cool effect for morphing photos DeltaPix 2007 VI Effects Color Post Acquire Process Window Distort Blur Moise Sharpen Calculations Edge Spatial Filters Force to Color Force Mona Invert Image 4dd Open Images Add Thumb Images F5 Fe F d d d F d d Freehand Wave Wind Polar 200m Wave Radial Wave Swirl Wave Freehand Shear 33 4 Idle Polar Effect Conversion Option 127 83 Fa AT Before Strength After Direction Angle Opacity i gt Before Cancel Exposed Area L Rectangular to Polar v Use Color Im We Cd 19 Page DpxView Pro Manual Zoom Wave distorts the bitmap with a wave ppm Wave dreri zoomed from any specified point on the bitmap Use the Radial Wave eff
66. ft Video 1 codec oy Some codecs are not compliant with the recorder If the recording doesn t start this may be a symptom of a non compliant codec Then try another codec or use uncompressed video The Stop button will stop recording AYI DeltaPixayi 2000 Record 10 fps US st C Hour Loi Sec C Min O Camera Add Date Time DeltaPix Duration Bed Mm er ms E Gei dn Playback 20 fps is 1782053 SI 9 gt Codec Quality 604 Microsoft Wideo v i gt L Uncompressed Micra Get Video oe Microsoft Video 1 s The AVI Capture button permits you to capture video to the selected active file Please note you should consider the hard disk usage when using this function For example the size of the AVI file by recording 27 frames per seconds with a video length of 98 6 seconds is 3 GB The recording frame rate can be set The recording frame rate can be defined using the slider The time base can set to Hour Min or Sec E g if the slider is set to 5 and the Min radio button is checked the video is recorded at 5 frames per minute If the Camera radio button is checked video is recorded at the actual full camera frame rate The recording duration can be set in the duration panel This allows for very long period recording at low frame rate The play slider sets the playback frame rate Long period videos
67. function helps to yield fast image displays The sensor region size is 1280 x 1024 3 4 13 Visualized Mode In this mode the sensor size is 640 x 480 This functions also yields fast image display but for a smaller region Reducing the sensor area will allow for higher frames rates 3 4 14 Preview Demosaicing This setting will change the quality of the live image display Higher quality will result in a higher CPU J Preview Demosaicing nee load and thereby cause a slower frame rate if the Bayer Color computer isn t sufficiently powerful Fast Low Quality Good Quality Best Quality 3 4 15 Color Correction Set in accordance with the illumination used to obtain a Tess correct color reproduction P Color Correction Template e Hal gt FI OF 1 By moving the slider to the right the screen color values Dash ie OAs Klee l 3 Daylight 2 None 2 Halogen 2 will turn to red and by moving it to the left the screen lz color values will turn to blue Color Offset y It is also possible to set the color values to their default values by clicking the left mouse positioned above the Cooler Neutral Wammer Neutral 3 4 16 Flip Image This function will move or offset the image in the x y or ai None both directions Flip Image Y xv 3 4 17 Reset camera If you want to restore the factory default setting or in the Sea rare event of the camera locking up select the Reset Reset S Camera
68. g can be done on images just after acquistion from camera The functions add some processing time It is possible to adjust the contrast and sharpness of an image before the capturing process 3 6 8 Window Camera Preview Window function allows you to shift to the live image preview Cascade command will connect several image zoom viewers to a single viewer Tile command will size each open image so that all of the images are visible on the Main Window This is a very useful function to compare images before and after editing DeltaPix 2007 VI Min Max Sharpen StdDey Clarify Color Adjust Close All Camera Preview Window Fz Cascade Tile 82 Page DpxView Pro Manual 4 Right Click Menu Right clicking on the mouse while positioning the mouse cursor anywhere on the image will activate the right click menu as shown to the right d Right click on the image and this menu displays when the annotation function is selected Click on an item to define how the various options will be handled Default Properties DeltaPix 2007 VI H Mame Line ROPS Transparent Transparent Color Fill Foreground Color Background Color Mote Background Color Redact Background Color Hilite Background Color Font Button Font Hotspot Metafile Encrypt Metafile Decrypt Metafile Ruler Modes Prot
69. gh resolution image at the preferred selected resolution 5 2 Resolution EE 1260 x 1024 Demosaic 1260 x 1024 Vu color 560 x 2048 Vu color S640 x 3072 Mu color 5120 x 4096 Vu color 3840 x S072 Ex color 2560 x 2046 Ex color 1260 x 1024 Nr color This function enables you to select the desired resolution of the image 5 3 Sensor Region The sensor region can be selected from this option The selected area is always centered on the mid point of the sensor leaving the outer parts unused Sensor Region Reducing the sensor area will allow for higher frames rates DeltaPix 2006 VI H H H H Grab Image Resolution Sensor Region F Exposure k White Balance F Black Frame Compensation Shading Correction F Color Correction Camera Settings Toolbar Video Capture Toolbar Main Menu Button Toolbar 4Subo hide Bukbonbar Window header Statusbar Live Image Tools k Full Screen Zoom a Exik program 640 x 480 DUU x 600 1024 x 766 1200 x 1024 1260 x 1024 2x2 subsampled Page 34 DeltaPix Camera Manual DpxView LE 5 4 Exposure Submenu The Auto entry will toggle the exposure function from Automatic to Manual Ee exposure or vICe versa Max Auto Exposure Time Max 50ms The Converge entry will initially set the E exposure function to Auto until the picture has settled leaving the exposure function in Max 1000ms Manual mode amp NoLimit AL ES S Suto
70. gt gt 5 2 million real pixels s 11 8 million real pixels 21 0 million real pixels AN AN ee AA a e New Modes 1 93840 x 3072 pixels 11 8 million real pixels DeltaEx color 2 5120 x 4096 pixels 21 0 million real pixels DeltaEx color These two modes have been developed recently allowing you to decrease the capture time of a high resolution image approximately to one fourth of the normal DeltaVu color using the DeltaEx color 3 1280 x 1024 pixels 1 3 million real pixels DeltaNr color This mode allows you to capture an image with automated black frame compensation function These choices are available only if you have set the video resolution to 1280x1024 Other video resolution selections i e the sensor area set in the Camera submenu in the Main Menu will result in other possible still image resolution settings The toolbar includes the following functions e Capture Button to capture a high resolution still image e Output Window to view the high resolution still image DeltaPix 2006 VI Page 41 DeltaPix Camera Manual DpxView LE 6 1 Capture Button Pressing the Capture senor ie will start the capturing process of a still image The progress of this is indicated at the bar at the bottom of the window Immediately following the capture process the DeltaVu calculations will start automatically and a new progress bar will be seen E m od Contrast 1 364 Brightness 0 662 _ i Gamma
71. he DP 200 and DP 300 cameras can be directly powered through the USB connection The Infinity X might need more power than specified in the USB 2 0 documents but can normally be powered directly If your computer doesn t supply enough power you can connect an external power supply with the following specifications 6V regulated DC plug with in the center outer plug diameter 5 6mm inner plug diameter 2 1 mm The DeltaPix CCD cameras DP 252 DP 253 and DP 450 require an external power supply with the same power specifications mentioned above This power supply will be enclosed with the camera 2 1 Software Installation The DeltaPix scientific USB 2 0 High Speed camera you have just purchased is designed to operate out of the box with minimal set up Prior to plugging the camera into the computer you must first install the software The software can be found on the CD ROM that was shipped with your camera 2 1 1 Minimum System Requirements Windows 2000 Service Pack 3 or Windows XP Service Pack 1 1 GHz Pentium Ill or higher compatible 128 MB RAM USB 2 0 Port Note An USB 2 0 Port is required The camera will not work on a standard USB1 1 port 2 1 2 Installation Procedure 1 If you are using a 3 rd party USB 2 0 PCI add in card please ensure the add in card is properly installed on your computer before proceeding We recommend that you use the built in operating systems USB 2 0 EHCI drivers provided by Microsoft rather tha
72. hed Bar Black and white Rendered Color Bar Font color Rendered Color Line Font color Rendered Gutosize Bar Keep relative Size Annotation Autosize Bar Keep relative Size end Di ier Jeng Chr se S DpxView Pro has an additional type of calibration bar Annotation Auto size Bar that enables you to resize and define the location of the calibration bar in the image 65 DeltaPix 2007 VI Page DpxView Pro Manual 3 5 1 Reporting When the measurements Angle Length Area and Perimeter are done it is possible to export the data to an Excel document Press the button E3 from the Annotation bar A new window will start up with the selected measurements Other options are available like inserting the image in the report type a header for the report and use DeltaPix excel template Template Use Excel Template C ProgranimersDeltaPis D ps emplate xls Sy DeltaPix template is located at DeltaPix folder Nobfu when exported Then press the Excel button to allocate the data Select save will start exporting the data A message will appear notifying you when the data is exported File C Documents and Settings Haissam El safadi Skrivebord Data xls created Ok The illustration to the right is the final report with logo header date and time data and image It is possible to do some modifications on the report like adding comments changing the l
73. hing different applications can be saved under individual file names and folders dk ak Ak 4h 4m aD Gain 1 296 Gain AGB gt gt To reload the settings at a later stage activate the IRR rence n function an h ropriate Profil Ge unction and select the appropriate Profile co amp amp xo Profile DPX File name Profile E Deen Y Files of type DeltaPix Profile P dp e Cancel When the appropriate Profile has been selected and the Open button circled in red has been pressed the following message is displayed DeltaPix Camera Preparing to restore settings From Profile profile wt Current settings will be lost continue anyway Pressing Yes will upload and restore the settings previously saved in the specified Profile file Mo Select No to cancel and continue with the current settings All settings are stored with the profile Because the visible buttons in the button toolbar are also saved the look and feel is also stored with the profile 3 1 4 Exit The Exit function will close the DeltaPix Viewer DeltaPix 2006 VI Page 8 DeltaPix Camera Manual DpxView LE 3 2 View menu The following options are available in the View File View Image Camera Settings Help menu 3 3 Camera Settings Toolbar Ctrl T Set if the Camera Settings Toolbar Video Capture Toolbar should be displayed or hidden ka Main Menu Set if the Video Capture Toolbar should K Butto
74. iew Pro e write of DFI information to image file is currently disabled Calibration data for this image will be lost and image may show wrong measurement data when reloaded Do you want to keep keep calibration by writing DPI to this image file Note To enable DPI information use menu Settings Magnification Embed DPI information in Image Yes Mo 57 DeltaPix 2007 VI Page DpxView Pro Manual The saved image is listed atthe top of 9 the thumbs as shown to the right 2 CS E Ee 1a Select a Directory To Browse in D mwe OE cobberosid DF 20x Srm tif Image jpg l o ferrit perlit 1 3m 20 DF ti Toluidin ipa Select a directory to browse your archive by activating junger mouse 11m vb H Toluidin2 jpg the Change Image Directory button A new dialog will in wo ee belle ipg h t th e ht Flowrescencel olecularProbe jpg Malte pg open as SNOWN to the rig STOBEJERN SYEROLITEN DIC 11M VERT mg Tceller jpg This archive contains some images in GIF bitmap Som JPEG and potentially other formats PE PEPE Al Select Selected Folder C Documents and Settings Documents DeltaPis magessImagesD It is possible to view archive the images as thumbs by pressing the Selec button The images will be listed to the meee left by name as shown in the ER figure to the right d Note that only TIFF BMP JPEG format are shown as thumbnails d
75. indow A 8 2 Output Window Capture high resolution image F1 Capture a snapshot of the Camera Open high resolution image window Window Increase high res resolution F2 Camera Preview Window Decrease high res resolution Ctrl A Undo All Processing Save application profile Work Space Ctrl C Copy Load application profile Work Space Ctrl F Fit Image to Window FastFocus mode on off Ctrl M Magnification Glass Visualizer mode on off Ctrl N Normal Full screen mode on off Ctrl O Open File Loop through high res resolutions Ctrl P Pan Window Toggle Black Frame Compensation on off Ctrl S Save File As Enable Auto Exposure to Converge Ctrl V Paste Toggle Auto Exposure on off Ctrl Z Undo Last Processing Fit to window Ctrl Alt A Area and Perimeter Gain RGB adjust Ctrl Alt F Snap Image to Window Ctrl H Help shortcuts Ctrl Alt Image Comment Ctrl L Live Image Ctrl Alt P Print CM Magnification Calibration Ctrl Alt R Realize Annotation Ctrl P Print Snapshot Ctrl Alt S Capture Screen Ctrl Q Questions and Answers Help Ctrl Alt Z Zoom Ctrl S Toggle Shading Correction on off Shift Del Cancel Region Ctrl T Ctrl X Camera Settings Toolbar on off Place Spot White Balance LLN Viewfinder on off CLAN Toggle Auto White Balance on off Ctrl Alt A Color Correction Ctrl Alt B Capture Black Frame Ctrl Alt C Snapshot Settings Ctrl Alt D Mini DICOM Im
76. installed see D Computer Management the device manager where the USB Dongle should SI Fie Action View Window Help appear am 6 Computer Management Local a VY Pibus ZS System Tools ae Processors E Event Viewer Sound video and game controllers Shared Folders System devices e Local Users and Groups d Universal Serial Bus controllers Si Performance Logs and Alerts E USB Dongles a Device Manager ES USB Dongle Software Protection Device _ SZ Storage Mi DeltaPix Discover the Details 3 1 2 Camera Check You can check that the camera is properly installed see the 42 Device Manager device manager where the DeltaPix camera should appear File Action View Help in this example Infinity X camera Ss E ze SS DELTAPIMHAISSAM Ki Batteries E E Computer GC e Disk drives E S Display adapters fl DYDYCD ROM drives E ial Human Interface Devices E E IDE ATAZATAPI controllers 1 Si IEEE 1394 Bus host controllers Imaging devices Sof Infinity USB 2 0 Camera e keyboard E E Mice and other pointing devices ie Modems E E Monitors E Sai Network adapters 8 PCMCIA adapters gt Pibus g s Processors H A Sound video and game controllers y System devices Universal Serial Bus controllers 3 1 3 External Power Supply Warranty void e Use only external power supplies from DeltaPix e The camera requires a 6v regulated power Some combin
77. isplay will be in real time without any latency delay or equivalent In a resolution of 1280x1024 you should be able to obtain 15 frames per second when the exposure time is faster than 60 ms With the resolution set to 640x480 you should be able to get over 50 frames per second if the exposure time is faster than 20 ms The actual frame rate is displayed in bottom of the DeltaPix software as shown below e 5120 x 4096 DeltaYu color we 100 En RGB 116 101 96 15 fps SET Connected CH 14 47 38 19 05 20 3 4 11 Frame Rate This menu is only available with CMOS cameras The Frame Rate submenu is available only if the Framerate mode 2 e Manual Frame Rate Mode is set to Manual 7 Frame rate K Adaptive to 50 Dee nena ee eae Adaptive to 100 Select one of the available frame rates The values for the frame rates are closely related to the exposure time Frame rate Sp lf one of the adaptive frame rate modes has been a 15 03 chosen this function is not available DeltaPix 2006 VI Page 16 DeltaPix Camera Manual DpxView LE 3 4 12 Fast Focus Mode Fast Focus Mode downloads the image data at a high speed and provides fast images for focusing and framing In this mode no computer processing is required and the data is sent directly to the video RAM for display on the monitor In addition the software automatically commands the camera to download only the pixels in a sub window that is centered on the sensor This
78. l Alt C Snapshot Settings Ctrl Alt D Mini DICOM Image Viewer ezDICOM Ctrl Alt F Focus Aid tool restart Ctrl Alt S Capture Shading Correction Frame Ctrl Alt T Toggle TWAIN Data Source on off Ctrl Alt X Exit Program Ins Increase Exposure Del Decrease Exposure Carlines Fast Increase Exposure Ctrl Del Fast Decrease Exposure Ctrl F1 Histogram and Profile 9 3 General Ctrl F Cirl U Ctrl D Fit to window Pan Up Pan Down Ctrl L ban Left Ctrl R Pan Right Ctrl Alt U Pan Fast Up Ctrl Alt D Pan Fast Down Ctrl Alt L Pan Fast Left Ctrl Alt R Pan Fast Right Page 48 www deltapix com DeltaPix Discover the Details DeltaPix Image Management Software Infinity X DP 200 DP 252 DP 253 DP 300 and DP 450 Cameras The contents of this document may not be copied nor duplicated in any form in whole or in part without prior written consent from DeltaPix DeltaPix makes no warranties as to the accuracy of the information contained in this document or its suitability for any purpose The information in this document is subject to change without notice Copyright 2007 DeltaPix All rights reserved DpxView Pro Manual M 1 Preface Thanks on behalf of the DeltaPix team for using DeltaPix scientific digital cameras The first part of the manual begins with hints to install DoxView Pro package and the dongle The second part is the DoxView Pro User and Operational Manual that invi
79. l DpxView LE 4 3 Calibration bar and magnification control Camera calibration is an important issue for many applications Some image formats keeps the magnification information like e g bitmap files Other file types does not store this information internally so a calibration ruler embedded in the image can be a convenient solution allowing for recalibration if the image is imported in third party programs The DeltaPix Viewer offers two easy ways of maintaining magnification information in the image 1 Calibrate one objective with automatic calculation for the other objectives This is done by interpolating and assumes that the nominal magnifications of the objectives are fairly precise 2 Each objective can be individually calibrated The software can save the calibration data as e g 10x 20x 40x and 100x according to the different objective lenses of the microscope 4 3 1 Configuring Magnifications First the magnifications to be used must be configured Two files in the DeltaPix installation directory can change the default magnifications in the Optics definition fields This allows display of all only the magnifications available on the microscope The files are flat ASCII files that can be Optics x 3 edited with any editor like Notepad They hold a list of magnifications plus the hint to be displayed on mouse over Below is a sample optics path1 Di file defining the hint as Microscope and definition of tw
80. lative to the Y axis and similarly for vertical direction shears y shear is measured relative to the X axis Empty triangles left over from shearing the image are filled with the background color defined by background color of the image 3 6 4 4 Canvas Resize Canvas Resize Position Canvas Resize dialog box has several features New width EM ET which will impact the final appearance of your canvas es creation New Height 1024 A Vertical Maintain Aspect Ratio f The absolute size of your canvas is controlled here Top Curent Width 1280 The Canvas Resize options are divided into main Curent Height 1024 BEEN groups size position background color and the current size The options are described below i Enter the values to set the corresponding width and height dimensions in pixels 71 DeltaPix 2007 VI Page DpxView Pro Manual If you want to see the canvas resize results immediately click on the Ok button to update the canvas display Check the box to maintain the current aspect ratio as you change either the height or width dimensions Increasing the canvas size adds pixels at the edge of the image Decreasing the canvas size hides pixels at the edges of the image full layer information is kept Horizontal and or Vertical allocation allows you to reposition the image Background color enables you to select another background color Finally the main dialog buttons are the
81. lgorithm V Si Auto Upgrade Camera To avoid unintended use you will be prompted for Enter the senal number of pour camera the serial number of the Infinity X camera ee n Cem The firmware download may take a few minutes i j Download configuration to camera This process may take a while and your system may look frozen You wll be notified when complete DeltaPix 2006 VI Page 21 DeltaPix Camera Manual DpxView LE Dprview DpxrView J Now ready to download Descriptor table to camera K Do wou want the new configuration take effect now ves Press the button OK to download Descriptor table to Select yes if you would like to activate the new the camera configuration The last step is to unplug and plug the USB 2 0 from DpxView the camera Pressing the button OK button will p d To make new configuration take effect unplug and plug the camera terminate the application Jj This program ts being closed now The new modes should be available now d Important Do not detach camera terminate the software or shut down computer while upgrading This may corrupt the camera firmware L Camera S Reset SC If an upgrade is wanted and the Auto Upgrade Camera menu no longer is visible then do a Reset All The Auto Upgrade Camera menu is invisible Then restart the software and the upgrade menu will now be available 3 5 3 Magnification Please refer to
82. mage changes and the algorithm tries to find where the images equals each other so it can calculate which part of each image that are in focus and can be used for the final image When the focus is changed also magnification and rotation can change and the image can be deformed This will depend on how much the focus is changed from one image to another If the changes are big the algorithm will have to accept bigger changes pulling the sliders to the right but that will also make the algorithm slower Normally the sliders need not to be changed but if you experience that the extended focus do not work well you can try and adjust them and see if it helps The Allow Re sampling allows the software to automatically resample the image to a higher resolution which sometimes can make the algorithm work better but this will also increase the calculation time Normally you should just use the default value The Calibration function is only for Z systems with limit stops as the macro stand Choosing the calibration function will make the software move the Z position to its two end positions and then it knows how many steps there are in the full travel range In that way the system can be calibrated in steps distance which is also very useful in combination with XY stages The Neutral position button move the Z stage to the middle position if the system is calibrated The Power Save button switches the power o
83. mance Logs and Alerts B i USB Dongles a Device Manager ES USB Dongle Software Protection Device Dal Storage v v 2 2 Camera Check You can check that the camera is properly installed see Device Manager the device manager where the DeltaPix camera should File Action View Help appear in this example Infinity X camera be amp 2 DELTAFIKSHAIS SAM Ki Batteries E Computer Se Disk drives a Display adapters 4 DYDVCD ROM drives Gka Human Interface Devices fy IDE ATAZATAPI controllers a IEEE 1394 Bus host controllers 35 Imaging devices Sop Infinity USB 2 0 Camera se Keyboards Mice and other pointing devices Re Moderns EI Monitors BS Network adapters E PCMCIA adapters gt Pibus ae Processors EI Sound video and game controllers System devices Universal Seral Bus controllers S i G i G i i sel se Rh Re Efe fe 53 DeltaPix 2007 VI Page DpxView Pro Manual 2 3 External Power Supply Warranty void Use only external power supplies from DeltaPix The CMOS cameras require a 6V regulated power and the CCD cameras require 5V regulated power Some combinations of PCs especially laptops and DeltaPix cameras may require an external power supply e We always recommend use of external power supply for all CCD cameras e If your Infinity X produces poor images this may also indicate that external power is needed The following messages
84. men in the light path One Shot Spot function gives you the possibility of using a single spot as reference for the white balance function by placing a square over a reference area in the image This reference can be any color chosen in the Color Target sub menu Normally only white or grey should be used unless you are very sure of the color you want to set as a reference The best result when using the spot white balance is achieved by selecting a non saturated gray area oY Selecting an area where one or more of the color channels are saturated may result in a weird color balance Automatic or Manual Exposure Control and White Balance the more pro active users will tend to use the SY manual mode whereas the less pro active will tend to use the automatic mode and let the software make the decisions for them Either way there is something for everyone 3 4 4 Snapshot Settings for CCD cameras DP 252 DP 253 and DP 450 DeltaPix CCD cameras offer snapshot capability This means that exposure and gain snapshot settings different from the preview settings can be used This is very useful e g for acquiring fluorescence images with long exposure It is possible to have a relative quick but lower quality preview frame rate by setting the gain high and enabling binning DeltaPix 2006 VI Page 11 DeltaPix Camera Manual DpxView LE Activate the snapshot window from the menu Camera Snapshot Settings or Ctrl Alt
85. n Toolbar be displayed or hidden Auto hide Buttonbar Set if the Main Menu Toolbar should be Si wicdewheadst displayed or hidden ES statusbar Set if the Button Toolbar should be Let Ctrl displayed or hidden Histogram and Profile Ctrl F1 tok e DICOM Viewer Ctrl Alt D Full Screen F9 Set if the Auto Hide Button Bar should be active Set if the Window Header should be displayed or hidden Set if the Status Bar should be displayed or hidden Set if the Live Image function should be active or disabled Click on the histogram function The histogram of the current image is displayed The graphic zone appears with the resulting histogram The Tools entry comprises an overall DICOM Viewer with limited functionality The viewer uses the de facto standard for viewing medical images Contrast and brightness can be adjusted by dragging the mouse over the image Set if you would like to view a Full Screen Image menu The mage menu includes the same features as File view Image Camera Settings Help located in the top of the Camera Toolbar i Starting the DeltaVu capture function een Capture 1280 x 1024 V4L_ Nr color E Starting the captured image viewer Increasing decreasing the resolution G of the DeltaVu capture gt Zou SS Higher resulution F3 Lower resolution F4 t A DeltaPix 2006 VI Page 9 DeltaPix Camera Manual DpxView LE 3 4 Camera Menu The Camera Menu shown to the right 3 4 1 Sensor Size You
86. n of a bitmap based image An image is up sampled to increase the resolution by adding new pixels An image is down sampled to decrease the resolution by throwing out pixels Resampling an image usually results in a loss of image quality because pixels must either be interpolated or thrown out The Bicubic Method is used for enlarging images Through a process called interpolation the bicubic method minimizes the raggedness normally associated with enlarging an image by estimating how the missing pixels should appear then filling them in with the appropriate color to smooth out the rough spots Use the Bicubic Method to enlarge photographic type images 3 6 4 6 Histogram The Histogram illustrates a graph representing the number of pixels at each level in an image figure to the right from black level 0 on the left of the graph to white level 255 on the right You can look at a histogram and quickly see the overall distribution of details contained in your image Mean 09 Lewet g7 Total breet SEO Madan Do Cami b173 Pecans 37 lad kA IK It is also possible to view the RGB histograms by reselecting the channel type red green or blue 72 DeltaPix 2007 VI Page DpxView Pro Manual 3 6 5 Effects 3 6 5 1 Effects Blur Motion Blur is an automatic tracking of every pixel from one Effects Color Post Acquire Process Window frame to the next There will be times when you want a motion ee E
87. n the device drivers supplied with your 3 party card 2 You must be logged into the computer with administrator privileges prior to continuing the installation 3 Close all application software that is running and then insert the DeltaPix Installation CD into your CD ROM drive If you are using a software update downloaded from our website please save the setup file in a directory of your choice Even that it is normally not necessary you are advised ad this point to uninstall previous versions of the software and hardware 4 Double click on setup exe the name on the setup program may vary or wait a few moments for the auto play function to run the setup program automatically if you are installing from a CD ROM II DeltaPix Discover the Details You should get the following windows dialog box CG DeltaPix InstallShield Wizard Welcome to the InstallShield Wizard for DeltaPix The InstallShield R Wizard will install DeltaPix on your computer To continue click Next WARMING This program is protected by copyright law and international treaties 5 Follow the onscreen prompts to install the software drivers and user application 6 After the software has been installed plug the USB 2 0 Camera into a free USB 2 0 High Speed port If this is not your first installation and you have not uninstalled your soft and hardware you should now be able to run the application called DeltaPix camera 7 I
88. nce function The Live Preview Button toggles between live video display On Off The CPU load is reduced if live video display is disabled If you run other programs together with the viewer you may experience slower response in these programs Disable the live video to free some CPU resources DeltaPix 2006 VI Page 31 DeltaPix Camera Manual DpxView LE Windows Windows Windows KE Professional Edition Windows MP Professional Edition Version 5 1 Service Pack Build 600 Verzion 5 1 Service Pack Z Build 600 CPU ES CPU 95 Class Piorrity Class Piorrity Realtime O Realtime A High C 7 High z Above Normal C 7 Above Normal Normal Gi Nommal C Below Normal C Below Normal The CPU usage when the video is OFF The CPU usage when the video is ON 4 6 Useful Buttons a Save and Open Work Space function enables you to save the current camera ia ki configuration such as exposure time contrast and brightness etc for later use with a similar application te Oper EI Save The Work Space function is a system setup for your personal applications Several profiles matching different applications can be saved under individual file names and folders AYI DeltaPikx avi d The Video Capture Button allows you to activate the AVI capture toolbar Tp ee ee Record SR 10 Ips UI d gt fpr Q Hour Sec O Min C
89. ntal or vertical object e g a calibration slide including a ruler press the left mouse key and drag it to the right end of the object finally releasing the p left mouse key Calibration Enter the Ruler Length and the Unit in the two Fuler length Unit fields shown in the figure below representing the 100 Moses known distance in the image Lens DPI is 61214 00 m to confirm the setting or x To initialize display of the calibration bar press Ki Press cancel to capture an image Press the magnify button to view the captured high resolution still image The calibration bar is now superimposed in the image as shown in the image to the right 4 3 2 Calibrate Objective Activate the Insert Calibration Bar in Images from the Settings Magnification menu Optics x 3 You can change the default magnifications in the optics definition field Double click on the first optic magnification will open a message box Select yes to open the ASCII file Type the name or identifier of the microscopes you are working with DeltaPix 2006 VI Page 28 DeltaPix Camera Manual DpxView LE Select File Save and close the ASCII file i e Optics definition files should be edited with great care To make the changes to take effects restart DeltaPix Jj To make the changes take effect the program needs to be restarted application or double click just outside the i magnific
90. nts Hints check box enables or disables text hints on mouse over a button 56 DeltaPix 2007 VI Page DpxView Pro Manual 3 2 1 Acquire Image Function b es ee zem gn imieniem pi Di You can capture a high resolution image either by selecting the capture S button from the Configurable button bar or from the DoxView Pro Button Menu EH A7 bar The live preview will shift automatically to the captured still image Ba Note that new functions become visible at the still image view like annotation below the DpxView Pro main menu CO OO ACS 1 ii Ee EEEE e Sai E e ASS SCH The live image window with the configurable menu bar could be hidden behind the still image It is possible to view both of them at the same window This can be done from the main menu Window Cascade or Window Tile 3 2 2 Save Image Function Save A File MEET FlourescenceMolecularProbe2 jpg Tceller ipg ae FlourescenceM olecularProbe jpg Timagel 1 jpg History STOBEJERN SYVEROLITEN DIC 11M VER ge Timagel2 jpg You can save the captured image by selecting the ca erh ee Save button A new dialog will open as shown to the WEE Ee ipg tt ipa ri ht Mjalte jpg TImage5 jpg g d Mialte2 ipa amp Timage2 ipg S H Desktop E Ki Type the name for the Image and press the Save Ca Gsm Fee button SE Save as type mace d Select Yes to keep calibration on Images Dpsv
91. o magnifications and some microscope names Please note that e Magnifications must have a leading gt character e tis also possible to enter a plain text like gt Zeiss gt Olympus gt Nikon or gt Leica in this way This makes it possible to manage multiple microscopes e The line with the leading will be hint for the pathi combo box Optics Pathi Configuration ZE This filet Optics pathk lt ctxt Edit with care SMicroscope Hint Pld gt 10 gt MyMicroscope gt Ze1ss gt Olympus gt Nikon gt Leica DeltaPix 2006 VI Page 26 DeltaPix Camera Manual DpxView LE Below is a sample optics path2 txt file defining the hint as Microscope and definition of two magnifications and some microscope names Please note that e Magnifications must have a leading gt character e The line with the leading will be hint for the path2 combo box e After calibration a new file will be created If Pathi is set to e g MyMicroscope the name of this new file will be optics path2 MyMicroscope txt This is done to allow a multiple set of calibrations Optics Path2 Configuration This fil Opties pathz txt Edit with care SMyMicroscope 70465 gt 0 8 PL E gt 1 6 You can get a set of sample files from DeltaPix support deltapix com Only magnifications in the camera path are to be entered The microscope eyepiece magnification is n
92. ocus Button The image to the right illustrates the output image of the extended focus calculation Please note that only images with the d object of interest must be open Other images of different objects must be closed d Please note that the extended focus calculation takes some time before the output image is ready 84 DeltaPix 2007 VI Page DpxView Pro Manual lf the output image suffers from quality right mouse click on the FOC extended focus button lt 2 a new window will open see figure to the right where it is possible to adjust the settings of the extended focus The settings relate to the amount of differences between two images with different focus Moving the sliders to the right will allow for big differences but also longer calculation times Moving them to the left will result in faster calculation times but then the algorithm might occasionally fail Normally the default mode is the best compromise d Please note that the manual extended focus calculation is limited with the options marked within the red box Automatic Extended Focus and Extended Exposure will be described later on this manual DeltaPix 2007 VI Extended Focus A adius 10 T Create QO gt x Close all sources g Auto close ee Default MER 10 Fallow Resampling E a Serre ra ve Show Hints D formation 4 us meting er di Automatic Extended Focus Automatic Z control Bidirec
93. ogo etc DeltaPix 2007 VI Export to Excel Comment Measure Angle 60 9 Length Diameter 5 7663 Perimeter 27 9168 Area 49 0209 a Image Header Insert Image E eltaFis Measurments amp Reporting Template Use Excel Template Destination Data Location Comment Measute DpxViewPro Excel Destination Gem i E Skrivebord L Dokumenter d Denne computer I Netvaerkssteder pro ED Genvej til DeltaPix EE lcenve til KundedatabaseNew xls Filnavn D ata xls Filtype Microsoft Excel xls 66 Page DpxView Pro Manual 3 5 2 Measurements on Live Preview Right mouse click on the preview button E Preview to do measurements on the live image in exactly the same way as on a still image It is also possible to export data to an Excel document 3 5 3 Image Recalibration Images acquired with a wrong calibration setting or images imported from elsewhere can be recalibrated Follow these steps e Draw ruler on a known reference The measurement possibly shows an incorrect value e While the ruler is selected type Shift Ins The recalibration windows will pop up e Enter the correct distance e The recalibration ruler will automatically go away e Measurements may now be performed 3 6 Main Menu The Main Menu in the still image window is shown below and has the following functions File E
94. on Setting wi Focus Aid wi Main Menu Auto bide Duttopbhar DeltaPix 2006 VI Page 24 DeltaPix Camera Manual DpxView LE 4 2 Exposure bar The Exposure bar enables you to control the camera Exposure 5m exposure When set to Auto exposure mode the slider is set in e H Ext accordance with the exposure function i Adjusting the slider marked with the red color to the right will make the image brighter dragging it to the left will turn the image darker In manual mode the slider sets the exposure time directly It Exposure Sms can be fine tuned by pressing the up down buttons at the right H Ext marked with the red color The exposure time is shown above the slider The Frame Rate Exposures is closely related to the exposure time short exposure time D M Ext high frame rate high exposure time low frame rate A long exposure time will limit the number of frames per second An exposure time of for e g 1 sec will limit the frame rate to 1 fps The general relation between frames per second and exposure time can be expressed with the following equation fps Maximum fps is also limited by the sensor read out rate ExposureTime By removing the check mark from the check box the Extended Exposure Sma Ext feature enables you to adjust and control the exposure A time easier in the low range from 0 5ms DeltaPix 2006 VI Page 25 DeltaPix Camera Manua
95. ose all Sources Auto close Sources Di On fe joo ba Enable Automatic lt Control _ Select the Zaxis Device Press Setup to configure the Stage Press the button Logon hil The Up Down Arrows Calibrate and Neutral Position buttons will turnon _ Select Calibrate to start runing the motor at the upper lower ends in TAJ Automatic Extended Focus Configuration Stage LI gt E Power Save Swap Up Down Avoid Dacklaah C Publish DOF Show Hints E00 Steps s Leg UI HUUIU UI 171 T Skartup C agan Calibrate ay Step order to find the middle point Press the Upper Arrow to start moving the translation stage along the optical axis diferent areas appear in focus in different images Press 1 button to select the top focus point and lt 2 button to select the bottom focus point Finally select the number of images to be captured automatically between the top and bottom focus points both included B Automatic Extended Exposure Configuration CI Finally press the button 1 Select the relative exposure time in percent FOC Create to start capturing high quality images with almost unlimited focus depth and exposure 88 Page DpxView Pro Manual 7 2 AZ Control Interface The different functions are as follows The five sliders are used to control how the extended focus algorithm should work Each time an image is captured with a new focus the i
96. ost Acquire Process pixels as the image changes size Different methods will at times Invert work better or worse depending on the image content Choose CMYK Hey HLS CMY window from Normal Resample or Bicubic The Bicubic method works Gray Scale i best with most images Adjust V i Transform j The Resample command is used to increase or decrease the size and or resolution of a bitmap based image An image is up Remap i sampled to increase the resolution by adding new pixels An Histogram d image is down sampled to decrease the resolution by throwing ost S out pixels Paint Scaling Normal Resampling an image usually results in a loss of image quality Count Unique Colors Resample because pixels must either be interpolated or thrown away cee The Bicubic Method is used for enlarging images Through a process called interpolation the bicubic method minimizes the raggedness normally associated with enlarging an image by estimating how the missing pixels should appear then filling them in with the appropriate color to smooth out the rough spots Use the Bicubic Method to enlarge photographic type images 3 6 6 9 Count Unique Colors Count Unique Colors command informs you about the number Unique Colors of the unique colors in an image Bitmap contains 239850 u DeltaPix 2007 VI nigue colors 81 Page DpxView Pro Manual 3 6 7 Post Acquire Process With this automatic processin
97. ot used in the calibration It is possible to customize the magnifications presented in the magnification drop down menus by editing the files optics path1 txt and optics path2 txt To use custom magnification options these files should be located in the DeltaPix installation directory Ask your DeltaPix distributor for more information on how to do this or contact DeltaPix at support deltapix com The following section demonstrates how the calibration bar works Example 10 SI we o wel y First select the values for magnification both magnification one and two Optics x 12 50 Then Fie wiew Image Camera Settings Help es Bo Calibration E Magnification Magnification Calibrate Ctrl M Images from ke Light source Frequency gt Ruler and Stamp Font Rendered Dashed Bar Black and white the Settings A S Embed DPI information in Image Rendered Color Bar Font color Magnif ication E rom Rendered Color Line Font color menu as Rendered Autosize Bar Keep relative Size shown in the figure to the right DeltaPix 2006 VI Page 27 DeltaPix Camera Manual DpxView LE The Ruler and Stamp Font option will enable you to choose your preferred color and font type and size Select Embed DPI information in Image It is important in order to keep your measurements values unchanged when saving the image Finally select the Magnification Calibrate option Position the cursor at the left end of a horizo
98. r to the right to zoom in to the left to zoom Cancel out or enter a value in percent manually in the white box 69 DeltaPix 2007 VI Page DpxView Pro Manual It is also possible to zoom in by pointing the mouse over an area and drag it To fit the image to the window just double click on the image 3 6 3 3 Magnification Glass The Magnification Glass follows the mouse movement The lens can be moved around the screen to magnify any area on the screen 3 6 3 4 Fit Image to Window Fit Image to Window function will resize an Image to fit your Project Window Itis also possible to Fit an Image to Window by double clicking on the image 3 6 3 5 Pan Window 274 The Pan Window displays a view of the main image at a smaller magnification and a rectangle indicating the size and position of the visible portion of the image The red rectangle can be dragged around in the pan window with the left mouse button to pan or move the image 3 6 3 6 Realize Annotation Realize Annotation makes the annotation a part of the image This is good e g when sending an image to a person without access to DpxView Pro 3 6 3 7 Area and Perimeter Refer to Annotation and Measurements 3 6 3 8 Image Comment Image Comment allows you to add your comments to the ape carmen image Type your comments in this box Type your comments in the box to the right and save the image 70
99. ractor Point Encrypt Fixed 83 Page DpxView Pro Manual M 5 DpxView Pro EF Extended Depth of Focus Manual Microscopy imaging often suffers from limited depth of focus However the object can be optically sectioned by moving the object along the optical axis different areas appear in focus in different images The Extended depth of focus function in DeltaPix software DpxView Pro EF can combine those images into one single sharp image If the images have different angle s to the optical axis as it is the case in stereo microscopes the software will automatically compensate for this The combination of the extended focus capability in the DpxView Pro EF software and the extreme high resolution capability of the Infinity X makes it easy to capture stunningly high quality images with almost unlimited focus depth ideal for research and routine work Example ak ee e ne Eet eee a i Maly pat Pe ree CS re Se ll E REE r EE e An F n a Capture a number of images with different levels of focus For example if the object of the interest has a depth of 1mm and the depth of focus of the optics of the microscope or macro lens is 0 25mm four images must be captured STEIER a FB rr Gg SCHEI EA pat EEN BS After capturing of the images you can activate the extended focus calculation by selecting the button below a ee Le FOC lt lt Extended F
100. rizontal For every input value HAG Hs TWN DI Factor point along the x axis there is a unique output value ES determined by the height of the graph at that point Re de y g grap p HH HHHH SH ac In the graph s default state the function is a straight SE line from bottom left to top right In this case each idle i 8 input value produces an equivalent output value and the graph has no effect There are a number of handles along the graph These provide the main means of interacting with the graph You can move them around arbitrarily subject to these two constraints the handles at the far left and far right of the graph can only be moved vertically and handles must remain between their neighboring handles for the graph to remain a proper function The handles are normally connected by a spline curve To see this move one of the handles by clicking and dragging it Note that the x y position of the current handle is displayed while the mouse button is 61 DeltaPix 2007 VI Page DpxView Pro Manual held down The function will remain a smoothly curved line that passes Ge all the handles You can change this behavior by putting the function into lines mode Select the function The function will change to a series of line segments that connect the handles Select the function to go back to spline mode 3 3 DpxView Pro Image Archive The DpxView Pro image archive contains three main tabs Thum
101. s mesons Force to Color Laplacian Force Mona F5 Sobel Invert Image F Prewitt add Open Images Shift and Difference Line Segment Add Thumb Images 76 DeltaPix 2007 VI Page DpxView Pro Manual Force to Color turns the color of the image to red green or blue depending on the selected color In Add Child Images at least two images have to be open This function allows you to add two images together In Add Thumb Images at least two images have to be selected e g by keeping the Ctrl button down This function allows you to add two thumbs together DeltaPix 2007 VI Effects Color Post Acquire Process W Blur Moise Sharpen Calculations Distort Edge Spatial Filters Force to Color Force Mona FS Invert Image F dd Open Images Add Thumb Images V b d d d V b Red Green Blue 11 Page DpxView Pro Manual 3 6 6 Color 3 6 6 1 Color Invert The nvert function enables you to change the color channels and thereby invert the entire image Not inverted Image Inverted Image 3 6 6 2 Color Gray Scale Grayscale color converts image data to the Grayscale Grayscale Color 3 6 6 3 Color Adjust Enter a value in the box figure below or use the scroll bar to adjust the brightness value of your image The scroll bar command gives you enormous flexibility in changing the brightness of your image Before Per
102. stalling Carl Zeiss MTB 2004 The program features you selected are being installed Please wait while the InstallShield Wizard installs Carl Zeiss MTB 2004 This may take several minutes Status Question InstallShield Cancel DeltaPix 2007 VI User Name my Name Organization My Organization Install this application For Anyone who uses this computer all users only for me Kenn Nielsen InstallShield ie Carl Zeiss MIB 2004 InstallShield Wizard Ready to Install the Program The wizard is ready to begin installation Click Install to begin the installation If you want to review or change any of your installation settings click Back Click Cancel to exit the wizard InstallShield The InstallShield Wizard has successfully installed Carl Zeiss MTB 2004 Click Finish to exit the wizard ial 91 Page DpxView Pro Manual 7 3 3 Configuring the Carl Zeiss MTB 2004 To make it work it may be necessary to configure the interface Start the MTB 2004 Configuration program and configure the microscope interface In the example below the microscope Camera Port is defined to a 1x adapter IE MTBConfig File Options KI Auto Configuration E B NewConfigurationt E kl Axio Imager 21 a 2 6x Motorized Nosepiece 10 Motorized Reflector Changer Motorized Focus gt lt No Tubelens Turret 25 Reflected Light Path Transmitted Light Path A Motorized Cond
103. t Stretch Intensity always retains the original number of different intensity values Ordinary contrast adjustments can lose values at the high and low ends of the scale The Histogram Contrast dialog to the right allows you to adjust the contrast of the currently displayed image Enter a value in the percentage box or use the scroll bar to set the percentage by which the contrast of the image should be increased or decreased Click the button OK to modify the image and close the dialog or click the button Cancel to close the dialog without modifying the image DeltaPix 2007 VI Histo Contrast e VK K 3 Before After Percentage 165 Page DpxView Pro Manual 3 6 6 7 Color Separate Separate Color The three RGB primary colors when Color Post Acquire Process mixed produce white but the three CMY primary colors produce black when they are mixed together Invert Window H Gray Scale j Since actual inks will not produce pure colors black K is S included as a separate color and the model is called CMYK GE Transform With the CMYK model the range of reproducible colors is Renan narrower than with RGB so when RGB data is converted to S CMYK data the colors seem dirtier EySFORreM e Separate Paint Scaling j Count Unique Colors 3 6 6 8 Color Paint Scaling Paint Scaling submenu allows you to interpolate the existing Color P
104. t it is highly dependant on temperature and so is difficult to eliminate reliably Image captured with exposure time 1 8s It shows annoying red green and blue hotspots Capturing a dark reference image with nothing but the Hot Spots displayed may help eliminating hot spots Use the Capture menu option in the main menu the Capture menu in the right click menu the shortcut or use the button in the button bar Prepare the black frame by shutting off all light or block the light path in the microscope When this has been done press the button OK Exposure time is 1 8s A new window informing that the Black Frame Compensation Frame has been captured will open DeltaPix Camera When the black frame image has been captured illumination may be turned on again and finally when selecting the Enable entry the static noise is eliminated from the image OS Due to the high temperature dependency of the hot spots you may have to repeat the procedure after a while Image captured with exposure time 1 8s Enabling black frame compensation has eliminated hotspots DeltaPix 2006 VI Page 13 DeltaPix Camera Manual DpxView LE 3 4 6 Shading Correction White shading can be a result of nonlinearities imp am in the optic path of a microscope or a lens The B acing Correction ice See EITENS result is that when pointing the camera atan Enable Ctrl evenly lit white object
105. t when renaming image files IS usab e i In Ge i Play Sound when image saved or opened CO n nectio Nn with Y Light source frequency gt High Resolution Image Stamps I fi H y X i Load Shading Correction file upon program start n init cam e ras e Windows Mf Warn when saving files with non persistent calibration information O n ly TWAIN Data Source gt Mf Reset Focus bar when Exposure changes Advanced Stay on Top Use Trayicon Directx Support gt EN Calibrate Camera Rabe Gamera d Backup Calibration Camera to File M Save Profiles as ASCII Restore Calibration File to camera White Balance algorithm gt i Enter License key Calibrating the camera is normally not necessary and requires special instruction not listed in this manual Only if the camera has been exposed to extensive mechanical stress recalibration may be needed You can verify if your camera needs calibration by making a high resolution snapshot of horizontal or vertical lines and check if the lines are absolutely straight or if they are jagged DeltaPix 2006 VI Page 20 DeltaPix Camera Manual DpxView LE This is an image from a camera which is not calibrated showing a black to gray transition on a horizontal line 1600xzoom Due to the misalignment the algorithms for processing the high resolution image will assume the image was captured at an incorrect position causing this type of image Should you experience such problems with your
106. tes you to learn how to manage and control DeltaPix cameras to the greatest advantage The manual will help you to learn about the flexibility and user friendliness of DeltaPix software Note All camera specific functionality is described in the DpxView LE manual This is available as pdf file on the software CD Alternatively it can be downloaded from www deltapix com Downloads Documents 50 DeltaPix 2007 VI Page DpxView Pro Manual Table of Contents 1 PREFACE cs gente ad et evra es Sac nace sae ied tue dee ouenenewessoucsusevoneetitedutieacanssvenewoievesnseetelsitiaucemnvateeels 50 2 SEQUENCE TO INSTALL THE DELTAPIX SOFTWARE ccs ccssssessssessseesseeesseesseeeenseeensenensenens 53 21 DONGE EE 53 22 ANNE GIN oon E nnnnacee Cranaeneccuieces 53 2 3 External e Tee UE 54 3 DPXVIEW PRO IMAGE MANAGEMENT SOFTWARE csscsssscssecssseeesseeesseeeenseeenseeensenenseeens 55 a NAVON ees eee eee ee ne ee eee ee eee 55 3 2 DPX VIEW Pro Button Mentee eege eege Eege 56 3 2 1 Acquire image FUNCION EE 57 SAV NIAC FUNCIO E 57 3 2 3 Change Image Dine CON Y esgecosetonsecedcneehonstioctsqesnndarsecasndotennescnashes tess exmsdecseuicnedeatudehoadetesectessncdeecanssenesiensel 58 SS WE Openlmage ie EE 59 3 2 5 KANNOT UNCION E 59 3 2 6 Viewin adSr FAAC ION BE 59 BF AdO Ge ONE EE 60 3 2 8 Merde FUNCION EEN 60 3 2 9 MENSI FUNCION EEN 61 3 3 ECKER EE IEN 62 3 3 1 TAUMDS EE 62 3 3 2 Klee EE 62 SE SC E D oio E
107. the Sum function 60 DeltaPix 2007 VI Page DpxView Pro Manual You can view the four images illustrated in the figure above in this way window using the Tile function This function is very useful to compare images before and after editing Add Bitmaps Preview SE Preview Window E Cascade Tile Another way to merge images is to keep the Shift button in your keyboard down and select the Merge button d Available List Selected List Please note that it takes some time to open the aech and Sotings Docd window to the right to merge the images e EE i x g i EEN v Select from the available list the images you would like i S i to merge Press the button OK to see the output Ents DeltaPix Images ImagesD aan 2nts D eltaPix mages ImagesD a Image ants DeltaPiz lmanes imanesl lt T gt Remove All Operation Average OK Cancel 3 2 9 Intensity Function KA Remap Intensity LS A The Intensity function allows you to change the brightness of the image change the contrast of the image and get some unique effects The Intensity graph is a function that lets you remap intensity values the Value component in the RGB or state HSV Color space into other intensity values SE SIE e sl The input and output values of this function both range BUSSE Curve Mode from 0 to 255 The input values range along the x axis rate e Cuve ei of this graph the ho
108. tional NI Device Ho Z an device Configuration Extended Exposure Enable 85 Page DpxView Pro Manual M 6 DpxView Pro EE Extended Exposure EXP Manual The Extended Exposure EE is a function used to ensure that images can be properly exposed especially in challenging lighting situations The Extended Exposure function in DpxView Pro EE works in the way that it combine multiple images each of them captured with different exposure time and having only a part of the image correctly exposed This can be necessary when the object under inspection has reflections or very dark and light areas in combination thereby making it impossible to expose the total image correctly with one single capture DpxView Pro EE can combine those images into one single sharp image Example ee ee ee CEET EE EE are Capture a number of images Mm each having a different Rp exposure or light level S WW After capturing of the images you can activate the extended exposure Bin m i sl calculation by selecting the Z button below S EXP gt Extended Exposure Button The image to the right illustrates the output image of the extended exposure calculation Please note that only d images with the object of interest must be open Other images of different objects must be closed Please note that the d extended exposure calculation takes some time before the output image is ready
109. u select the functions you need and hide the functions you do not need The default appearance is shown below and includes shortcut icons to the commonly used adjustments on video and image view Exposure 10ms Optics x 20 Focus OR E Restart Xe e P l me pA Je e ee Ipnnnnngnngnnngnnggi 4 1 Configuring the button toolbar It is possible to configure the Button Toolbar icons by right clicking the mouse in the area marked with red color or direct click on the button marked with green color as shown below Exposure 10ms Optics x 20 Focus be pes GEN Met ek e Restar Oy Be Wo RQ EP WB sic M Zilsnnnnnnnnnngnsnn eno Bs ep A new menu will appear shown in the figure to the right and allow for Ra Live Preview configuration of the button toolbar Ra Grab And Process bw Camera Toolbar Adding new functions by ticking them will add new icons to the Button Fast Focus Fast Update Toolbar representing the new functions e Save or Open Workspace gett UE ew a Opecs Ie 28 Pocus Beta ES r am Ki exe WB i Ki S COE T on 1 d itt a DAT Capture Toolbar a High Resolution Still Image kT Auto Exposure OntOFfF Exposure Converge Exposure Reset Auto White Balance On Oft ke white Balance Spot Black Frame Compensate Shade Correct Save Snapshot Print Snapshot Histogram and Profile Snapshot Settings Optics Calibrate fe Buttons opoft wi Exposure Bar Magnificati
110. ursor position e 2560 x 2048 DeltaVu color SI Ta 100 D RGB 9 13 26 El St St Connected C 11 44 28 AM 7 4 2004 The active Still Image resolution The active Zoom Image mode Shows the level current frame rate in frames per second or as here Still if Live Preview is off The Status bar can be hidden from the View menu DeltaPix 2006 VI Page 43 DeltaPix Camera Manual DpxView LE 8 High Resolution Image Viewer The High Resolution Image Viewer can View LE Still Image be accessed from the camera toolbar or from the image menu File View Zoom Effects Batch Process Undo Camera Window Fe The Image Viewer will start showing the latest captured image Within the image viewer you will also see a new menu bar 8 1 File Menu The file menu gives you basically the same functions Eile as in the file menu in the Live Image Viewer with Open Image Chr two functions added Save Image Chrl 5 Upload Image as d EE Print Image Ctrl F Clicking the Open Image option will allow you to open previously saved images in various file formats Upload image sub menu shown below will allow BB you to upload an image to a previous selected FTP server refer to the FTP Client sub menu Open Image Cl Save Image Chr Upload Image as L JPEG po Print Image Ctrl P nm TP Configuration 8 2 View Menu The view menu allows you to select File View Zoom Effects Batch Process Undo Camera Win
111. w Meade E 39 DiJ NAMES BRAN esciisc cece cecse EERE aeaa EEEa RE EEE aa 39 515 Live MIN e LE 39 SE TOOR aia E A E EEE E E E E A A A E 39 5 17 TITTEN video Re EE 39 S10 LOOM E 40 S19 LEE EE 40 6 CAMERA TOOLBAR sees ects sect ecco eat ccc mete ainiai aaaeaii 41 Ol Capture TT e DEE 42 Ce TE DA WW E 42 63 Camera STUN S E 42 DeltaPix 2006 VI Page 3 DeltaPix Camera Manual DpxView LE 1 OTAPUS BAR oiie er nen eee eer ere eee eer aera eee eee 43 8 HIGH RESOLUTION IMAGE VIEWER ccscsssssesssssssessesssssessesesseseeseeseesessesaesaesoeseesoesseseeseesesaesanees 44 or FIG IMG E 44 OL VCW TEE 44 SO ZOOM d En TE 45 A EREC MONM eege Ee 45 89 Batoh PIOCESS Menu EE 46 B0 SEENEN 46 or Camera VV INGO EE 46 8 8 Right el el ennnen ennn nnnn nunne nnmnnn nnmnnn nannan nenen 46 o9 TXANO UOD o E AOE OO A A 46 8 10 Black eege 47 Et SEET 48 or ele Le E 48 OK 48 EC C lt i enn a nee eee ee eee 48 DeltaPix 2006 VI Page 4 DeltaPix Camera Manual DpxView LE M 1 The DeltaPix Viewer The DeltaPix Viewer is a user friendly tool allowing you take full advantage of your DeltaPix camera The software contains the basic functionality to control the camera acquire high quality images and save the images In many cases this software is all you need to enter the world of digital microscopy The DeltaPix Viewer allows the user to capture view manipulate save and load images in various formats like windows bitmap jp
112. witch Ele view Zoom Effects f tch Process Undo Camera Window F2 back to the camera window 8 8 Right Click Menu Right clicking on the mouse while pointing it anywhere on the output image opens the options listed to the right Zoom e Lt Save Image be Load Image hd Ka Grab and Process nnotation j Ruler and Stamp Font 8 9 Text Annotation Text can be added to still images Press the Al oss l iti 2 L Ih lecower th i ss D I Please select a zoom factor equal to or above 100 before doing annotation DeltaPix 2006 VI Page 46 DeltaPix Camera Manual DpxView LE M The Color button allows you to select the desired font color on the image SS The Font button permits you to select the desired font type and size r T This button confirms your selected choices with annotation This button cancels the annotation process E Use the Undo or Undo All to get rid of misplaced text 8 10 Black Level Clamp When acquiring e g fluorescence images it is often desirable to have a uniform black background To make a region black and all regions in the images with intensity lower than the actual regions intensity 10 place the cursor in the region and Shift Ctrl Click Place the cursor at the region and Shift Ctrl Click The resulting image becomes the figure to the right G This Black level clamp is only available in the DpxView L
113. y as described in the previous section Enter the Ruler Length and the Unit in the two fields representing the known distance in the image Pressing the button OK will open a message box DpxView Pro eh Interpolate calibration of all objectives From this 4 00 objective calibration Select Yes to confirm the interpolation Be It is recommended NOT to do this but calibrate objectives individually calibration of all objects from 4x To EE magnifications YES Mo Select No to calibrate each objective individually DeltaPix 2006 VI Page 29 DeltaPix Camera Manual DpxView LE It is recommended to calibrate objectives individually and avoid interpolation However if your objectives are close to their nominal magnification or you don t need high precision for your calibration bar and measurements then use interpolation and save some time avoiding calibration of all objectives Selecting Yes will result in opening a MATIE new warning dialog box Select yes to a WARNING proceed and inte rpolate calibration to a 1 Interpolating this x4 00 objective calibration to all other objectives will overwrite any exixting calibration For the all other objectives 2 Using interpolated calibrations may be unprecise if nominal magnifications are not precise Proceed ayway select No to calibrate each objective vs II Ne individually If you decided to use the interpolated calibration then you are done by now
114. y has been 1600 assigned to another function 3200 Fit to Window 8 4 Effects Menu The Effects menu includes various image oie on a ae rm ee processing and filtering functions Color Adjust PA t Ez Contrast bk Process RGB Seperately PE d D e Flip will flip the image e Color adjust allows you to adjust the color balance manually e Contrast lists various contrast adjustments Big Edge Borderize Define Edge Detect The remaining options represent different kinds of standard filters with various effects which may suit your application Emboss Highlight NW Light Old Stone gt Outline Sharpen gt Clarify Median Filter gt Custom Filter gt Invert Image F5 Force mono F6 DeltaPix 2006 VI Page 45 DeltaPix Camera Manual DpxView LE 8 5 Batch Process Menu The Batch Process menu enables you File View Zoom Effects Batch Process Undo Camera Window Fe to select a number of functions from Contrast the effects menu to be activated e automatically when the High sharpen Resolution Image Viewer is opened Clarify Color Adjust Custom Filter The selected functions will be processed according to the settings in the Effects menu 8 6 Undo Menu The undo menu lets you undo the last File View Zoom Effects fdbch Process Undo Camera Window F2 effect applied or all of them All Crit 8 7 Camera Window Finally clicking the Camera Window will s

DeltaPix Camera & Software Installation Manual DpxView LE

Contents

Download Pdf Manuals

Related Search

Related Contents

DeltaPix Camera &amp; Software Installation Manual DpxView LE

Contents

Download Pdf Manuals

Related Search

Related Contents

DeltaPix Camera & Software Installation Manual DpxView LE