OPERATING INSTRUCTIONS Cytocentrifuge Cellspin I
Contents
1. Instructions Cellspin 31 12 OPTIONAL ACCESSORIES Cellfunnel reusable 39910000 12 pcs Cellfunnel disposable 399 10004 100 pcs 39910005 500 pcs Filtercards for Cellfunnel reusable 39910001 Standard 200 pcs 39910006 Thin for samples less 0 4 ml 200 pcs Cellslides Single circle uncoated 39910002 100 pcs Cellslides Single circle coated 39910003 100 pcs Double Cellfunnel reusable 39910010 12 pcs Double Cellfunnel disposable offset 39910014 100 pcs 39910015 500 pcs Filtercards for Double Cellfunnel reusable 39910011 500 pcs Cellslides Double circle uncoated 39910012 100 pcs Cellslides Double circle coated 39910013 100 pcs Cellslides Double circle offset For Double Cellfunnel disposable only 39910016 100 pcs ECOfunnel reusable 39910020 12 pcs ECOfunnel disposable offset 399 10023 100 pcs 39910024 500 pcs ECOseal for ECOfunnel 39910021 100 pcs ECOslides coated 39910022 100 pcs Cellclip for Cellcliprotor 39910031 1 pc Instructions Cellspin 32 13 WARRANTY SLEE Medical GmbH guarantees that the product delivered has been subjected to a comprehensive quality control procedure and that the product is faultless and complies with all technical specifications and or agreed characteristics warranted SLEE Medical GmbH guarantees that the instrument is manufactured under an ISO 9001 quality2. bottom of the housing Position the centrifuge a stable and level manner in a suitable place Connect the power line of the instrument to the power outlet on the rear Turn on the mains switch Switch position I The last used centrifuge data will be displayed Open the lid Instructions Cellspin 7 INITIAL OPERATION 7 1 DISPLAY AND CONTROL PANEL Software settings and motor operation can be operated with the control panel on the ma GD Gi front side of the instrument bolle R RPM RCF x 100 44 00 090 Speed A numeric value of 500 RPM up to the maximum rotor speed can be set Preset in steps of 100 RPM displayed value x 100 If the key or is kept pressed the value changes with increasing speed Display the brake step and the centrifuging radius Running time Preset from 1 99 minutes in 1 minute steps Continuous operation Centrifuging radius Input in centimetres Preset from 5 16 centimetres in 1 centimetre steps Braking steps O or 1 Step 1 short run down time Step O long run down time If the key 4 or is kept pressed the value changes with increasing speed Start centrifugation run End centrifugation run The rotor runs down with the preselected brake step Save the brake step and the centrifuging radius Display of the relative centrifugal force RCF The display of the relative centrifugal force RCF appears while the key RCF is kept pressed Shor
3. part of the closed rotor with the other hand upon lifting of the lid Removal of closed rotor out of the centrifuge Unlocking and lifting of rotor lid Insert Cellfunnels or ECOfunnels into the rotor ATTENTION Always load the rotor symmetrically Position lid centred onto the rotor and press until you here the activation of the lock click sound Place closed rotor centred into the centrifuge Ensure that the safety pin locks into holder at the centrifuge hub Loading of closed rotor Closing of rotor lid Instructions Cellspin 13 Cleaning of closed rotor Do not clean the closed rotor in the dishwasher or with aggressive chemicals or cleaning solutions Step 1 Open the rotor Step 2 Remove the four screws at the centre of the rotor Step 3 Litt the Cellclip holder trom the rotor Step 4 Remove the gasket trom the outer rotor Instructions Cellspin Step 5 Clean and disinfect all components i e rotor Cellclip holder lid and gasket Dry the components before reassembly Step 6 Assemble the Cellclio holder centred onto the rotor Ensure that the guiding pin locks into the respective opening the rotor Step 7 Fasten the four screws in the centre of the rotor Step 8 Grease the pivot a little bit Step 9 Place the gasket onto the outer ring of the rotor Ensure that the gasket is evenly fixed Always Till the centrifuge containers outside of the centr
4. to the appropriate cell concentration Instructions Cellspin 25 Centrifugation speed time and acceleration The majority of users utilize a centrifugation speed of 500 to 1 500 rom for 10 minutes This is sufficient for most samples The higher the protein load of the sample the higher the centrifugation speed and the longer the centrifugation time should be selected Crucial for optimum results is the complete absorption of the liquid by the Tiltercards and stable fixation of the cells to the surface of the glass microslides If the centrifugation time is selected too short remaining sample fluid will wash off the cells from the surface of the microslide Too long centrifugation times will result in drying of the cell and potential damage to the cell structures This can lead to wrong diagnostic results Examples Sample type Centrifugation time Centrifugation speed minutes Kidney puncture 10 1000 Lavage washes 10 1000 Liquor 7 1000 Mamma puncture 10 1000 Pleural fluids 12 800 CD4 or CD8 lymphocytes 5 500 Urine 10 1000 Do not Tix the slides in 95 alcohol before complete drying of the microscope slides Failure to comply results in washing off the cells trom the glass surface Instructions Cellspin 26 Cellspin conversion table Speed RPM min Instructions Cellspin 27 10 CLEANING AND MAINTENANCE 10 1 CLEANING The recommended frequency of cleaning of the cy
5. OPERATING L medical INSTRUCTIONS Cytocentrifuge Cellspin INS9100GB 12 2010 03 Instructions Cellspin 1 Instructions Cellspin CONTENTS 1 INTENDED USE 2 SYMBOLS 3 SAFETY NOTES 4 COMPONENTS 5 SPECIFICATIONS 6 UNPACKING AND INSTALLATION 7 INITIAL OPERATION 8 OPERATION OF CYTOCENTRIFUGE 9 APPLICATION NOTES 10 CLEANING AND MAINTENANCE 11 SERVICE 12 OPTIONAL ACCESSORIES 13 WARRANTY 14 DISPOSAL Instructions Cellspin 1 INTENDED USE cytocentrifuge Cellspin is intended for separation and deposit of a monolayer of cells on microscope slides for professional use in routine and research laboratories in the fields of biology medicine and industry The system consist of a centrifuge a specific rotor Cellcliprotor and a sample preparation system composed of a cellclip a microscope slide a filter card and reusable or disposable cell funnel Cellclip Microscope slide Filter card Cellfunnel 2 SYMBOLS Dangers warnings and cautions are marked by this symbol Special instructions regarding the operation of the instrument are marked by this symbol Instructions Cellspin 3 SAFETY NOTES Before the initial operation of your centrifuge you should read and pay attention to the operating instructions Only personnel that has read and understood the operating instructions are allowed to operate the dev
6. argin of 300 mm around the centrifuge The centrifuge must not be moved or knocked during operation In case of fault or emergency release never touch the rotor before it has Stopped turning To avoid damage due to condensate when changing from a cold to a warm room the centrifuge must either heat up for at least 3 hours in the warm room before being connected to the mains or run hot for 30 minutes in the cold room Only the rotors and accessories approved by the manufacturer for this device may be used The centrifuge may only be operated when the balance 15 within the bounds of acceptability The centrifuge may not be operated in explosion endangered areas The centrifuge must not be used with inflammable or explosive materials or materials that react with one another producing a lot of energy users have to centrifuge hazardous materials or compounds contaminated with toxic radioactive or pathogenic micro organisms they must take appropriate measures Repairs must only be carried out by personnel authorised to do so by the manufacturer The following safety regulations apply IEC 61010 1 and IEC 61010 2 020 as well as their national deviations Instructions Cellspin 4 COMPONENTS SLEE cytocentrituge Cellspin is provided with the following standard components Cellspin Operation manual e Cellcliprotor 12 positions 12x Stainless steel Cellclips applicable for reusable and disposa
7. ble Cellfunnels Instructions Cellspin 5 SPECIFICATIONS Power supply Power Consumption Chamber volume Rotor Slide Capacity Max RPM speed Running time Safety Dimensions width x depth x height Weight without accessories Instructions Cellspin 208 240 V 50 60 Hz 300 VA Cellfunnel Single 0 5 ml per chamber Double 0 5 ml per chamber ECOfunnel 5 ml Cellcliprotor Up to 12 microslides Cellcliprotor 2 000 min Centrifuge with applicable rotor 6 000 1 99 minutes continuous run short cycle mode impulse key Imbalance switch oft Automatic rotor recognition Lid locking and holding 366 mm x 430 mm x 257 mm 24 kg 6 UNPACKING AND INSTALLATION 6 1 UNPACKING THE INSTRUMENT Remove the upper cover Remove the upper supporting foams Lift the centrifuge on both sides with an appropriate number of helpers and place it on the laboratory table For repacking use the original cases Keep the packing material 6 2 INSTALLATION SITE REQUIREMENTS The site for installation should meet the following requirements e Place the instrument onto a vibration free level surface e No direct sunlight onto the instrument No sources for heat or cold in the proximity of the instrument e minimum distance of 10 20 cm between the instrument and walls or other laboratory equipment 6 2 INSTALLATION Remove the transportation satety device trom the
8. controls After use dispose filter cards in accordance with applicable regulations Instructions Cellspin 20 8 7 SETTING THE BREAK STEP Switch off the mains switch Keep the key beneath the speed indicator and the key IMPULS pressed simultaneously Switch on the mains switch and release the keys again The speed indicator shows the machine version and the time indicator shows the set brake step e g ee 0000 If the machine version and brake step not displayed press the key under the speed indicator until they are displayed The machine version 15 set by the manufacturer and cannot be changed Set the desired brake step with the keys beneath the time indicator Step 1 short run down time Step O long run down time Press to save the setting 8 8 CENTRIFUGATION WITH PRESELECTED TIME Set the desired speed with the keys 7 beneath the speed indicator Set the desired time with the keys beneath the time indicator Press The rotation indicator appears while the rotor is turning The time is displayed in minutes The last minute is counted down in seconds When the time is displayed in minutes a point flashes next to the number After expiry of the time or if the centrifugation run is interrupted by pressing the rotor runs down with the set brake step During the centrifugation run the rotor speed or the resulting RCF value and the remaining time are displayed Instructi
9. es of hygiene and it also prevents corrosion through adhering impurities In the event of condensation water formation dry the centrifugal chamber by wiping out with an absorbent cloth If infectious materials penetrates into the centrifugal chamber this is to be disinfected immediately Lightly rub the rubber seal of the centrituge chamber with talcum powder or a rubber care product after each cleaning Rotors and Attachments In order to prevent corrosion and material changes rotors and accessories must be cleaned regularly with soap or a mild detergent and a damp cloth Cleaning is recommended at least once a week even better after every usage the rotor or accessory parts are contaminated by pathogenic material a suitable cleaning has to be executed The rotors and accessory parts must be dried immediately after cleaning Angle rotors container and hanger made of aluminium are to be lightly greased after drying using acid treen grease e g Vaseline The sealing ring is to be replaced immediately upon indication of crack formation embrittlement or abrasive wear In order to prevent the packing ring from twisting when opening and closing the cover the packing ring must be lightly rubbed with talcum powder or a rubber care product Instructions Cellspin 29 In order to prevent corrosion as a result of moisture between the rotor and the motor shaft the rotor should be disassembled and cleaned at least once a m
10. ice 3 1 SAFETY FEATURES The SLEE Medical cytocentrituge Cellspin is provided with the following safety features Cellspin Imbalance control rw Alarm functions 2 The institution which owns the unit and the persons working with the unit servicing or repairing it have the responsibility for a hazard tree use 3 2 ELECTRICAL POWER CONNECTION Do not use any extension lead Make sure that electric power is constant e This should be examined during installation of the unit by a competent person e Use a dedicated fuse for the unit Do connect another unit to the same power circuit e Before turning on the instrument check if the voltage of the mains supply is identical with the name plate of the unit Instructions Cellspin 5 3 3 WORKING WITH CENTRIFUGES Along with the operating instructions and the legal regulations on accident prevention you should also follow the recognised professional regulations for working in a safe and professional manner This centrifuge is a state of the art piece of equipment which is extremely safe to operate However it can lead to danger for users or others if used by untrained staff in an inappropriate way or for a purpose other than that it was designed Tor Before using the centrifuge absolutely check the rotor for firm placement When the centrifuge Is running according to IEC 61010 2 020 no persons dangerous substances or objects may be within the safety m
11. ifuge e No liquid should be allowed to enter the centrifugal chamber during filling and swinging out of the hangers The maximum filling quantity for Cellfunnels ECOfunnels specitied must not be exceeded In order to maintain the weight differences within the centrifuge container as marginal as possible a consistent Till level in the containers Is to be heeded Instructions Cellspin 15 8 3 PREPARATION AND USE OF CELLfunnels Step 1 Open the Cellclip Step 2 Insert microscope slide with labelled side away trom bracket Step 3 Insert filter card Step 4 Insert Cellfunnel All Cellfunnels fit into the Cellclip Step 5 Fix the assembly by pulling the brackets together and hook them into the holder Step 6 Insert the assembly into the rotor The Cellfunnel chamber should face into the centre of the rotor Step 7 Open the Celltunnel lid and pipette 0 5 ml of sample into the chamber Close the Cellfunnel lid Instructions Cellspin Step 1 Step 5 Step 6 Step 7 Step 8 Close the centrifuge lid Set centrifugation time and centrifugation speed Press Start After centrifugation Open the centrituge lid Take the Cellfunnel assemblies out of the rotor Step 9 Open the Cellfunnel assembly by pulling the brackets out of the holder and simultaneous pressing of the Cellfunnel onto the microslide Step 10 Remove Cellfunnel and dispose dis
12. ket out of the holder Keep assembly upside down to avoid flow of excessive sample onto the microslide Step 10 Hold microscope slide and ECOTunnel seal with one hand the ECOtfunnel with the other Keep assembly upside down to avoid flow of excessive sample onto the microslide Step 11 Remove ECOfunnel and dispose disposable or put it into a disinfecting solution reusable Instructions Cellspin Step 7 Step 10 Step 11 Step 12 Remove microscope slide and seal together from the Cellclip Step 12 Step 13 Carefully remove ECOfunnel seal from the microscope Slide Stain microscope slide Step 13 8 5 CLEANING AND DESINFECTION OF REUSABLE ITEMS Reusable Cellfunnels and ECOtunnels as well as Cellclips should be decontaminated and cleaned after usage The use of standard surface disinfectants is recommended After disinfection Cellfunnels ECOtunnels and Cellclios should be cleaned in water and dried Do not use bottle brushes or aggressive chemicals Failure to comply may result severe damage to the items Cellfunnels ECOtunnels are considered for repeated use Upon long term use the inner surface of the chamber will roughen After two years an exchange of reusable Cellfunnels and 5 is therefore recommended 8 6 DISPOSAL OF FILTERCARDS Filtercards are intended for single use only All materials are considered contaminated with patient specimens or
13. management system Unauthorized modification or repair by third party persons will void the warranty Only original SLEE spare parts must be used Guarantee claims can be put forward only if the instrument is used according to this manual and for the purpose described Mistakes and errors which occur because of improper use cannot be accepted 14 DISPOSAL The instrument or parts of the instrument must be disposed of according to existing local applicable regulations Instructions Cellspin 33 Instructions Cellspin Intentionally left blank 34 Instructions Cellspin Intentionally left blank 35 Instructions Cellspin SLEE medical GmbH Carl Zeiss Str 49 55129 Mainz Germany Tel 49 61 31 9 58 71 0 Fax 49 61 31 9 58 71 722 mail slee de http Awww slee de 36
14. ons Cellspin 21 8 9 CONTINUOUS OPERATION Set the desired speed with the keys Y beneath the speed indicator Set the time to zero with the key beneath the time indicator is displayed Press The rotation indicator appears while the rotor is turning The time count Starts from 0 The first minute is counted up in seconds and then the time Is displayed in minutes When the time is displayed in minutes a point flashes next to the number Press S to end the centrifugation run The rotor runs down with the set brake step During the centrifugation run the rotor speed or the resulting RCF value and the expired time are displayed 8 10 SHORT TIME CENTRIFUGATION Set the desired speed with the keys beneath the speed indicator Keep pressed The rotation indicator appears while the rotor is turning The time count starts from 0 The first minute is counted up in seconds and then the time Is displayed in minutes When the time is displayed in minutes a point flashes next to the number Release again to end the centrifugation run The rotor runs down with the set brake step During the centrifugation run the rotor soeed and the expired time are displayed Instructions Cellspin 22 8 11 DISPLAY OF THE RELATIVE CENTRIFUGAL FORCE The relative centrifugal force RCF can be displayed during the centrifugation run If the relative centrifugal is used the cent
15. onth and the motor shaft should be lightly greased The rotors and accessory parts are to be checked on a monthly basis for corrosion damage Rotors and attachments may no longer be utilised upon indication of wear and tear or corrosion Check the firm seating of the rotor on a weekly basis Rotors and accessories with limited term of use The use of specific rotors suspensions and accessories is time limited They are marked with an expiry date e g einsetzbar bis Ende usable until end of IV Quartal 2011 applicable until the end of IVth quarter 2011 The rotors suspensions and accessories may not be used for longer periods for safety reasons once the marked expiry date has been reached Instructions Cellspin 30 11 SERVICE Internal components should only be serviced by technicians authorized by SLEE If technical service or spare parts are necessary please contact your local SLEE Medical distributor Please have the following information available Complete contact details Type of instrument and serial number Place of instrument and name of user Purpose of service call Delivery date of the unit If it is necessary to return the instrument it must be cleaned and disinfected before delivery It must be returned in its original packing If the instrument or parts thereof sent back in a dirty or non disintected condition SLEE reserves the right to return the parts to the debit of the customer
16. posable or put it into a disinfecting solution reusable Step 11 Press thumb on the labelled areas of the microslide and carefully remove the filtercard Step 12 Take out the microslide for further staining and incubate Cellclip in disinfecting solution Step 11 Instructions Cellspin Step 10 Step 12 8 4 PREPARATION AND USE OF ECOfunnels Step 1 Open the Cellclio and insert microslide with labelled side away trom the bracket Step 2 Insert ECOfunnel seal onto the microscope slide Step 3 Insert ECOfunnel Step 4 Fix the assembly by pulling the brackets together and hook them into the holder Step 5 Insert the assembly into the rotor The ECOfunnel chamber should face into the centre of the rotor Step 6 Mix sample gently and check the turbidity of the sample Depending on the turbidity pipette approx 1 2 ml into the ECOfunnel chamber Instructions Cellspin Step 1 Step 2 Step 3 Step 4 Step 6 Step 7 Close the ECOfunnel lid Step 7 Close the centrifuge lid Set centrifugation time and centrifugation speed A centrifugation at 1200 rom for 5 minutes is recommended Press Start Step 8 Open the centrifuge lid after centrifugation Take the ECOfunnel assemblies upside down out of the rotor to avoid flow of excessive sample onto the microslide Step 9 Press ECOfunnel onto the assembly and open Cellclip by pulling the brac
17. rifuging radius must be entered Keep pressed during the centrifugation run The relative centrifugal force RCF appears in the speed indicator RCF displayed value x 100 Release again The speed is displayed RELATIVE CENTRIFUGAL FORCE The relative centrifugal force is given as a multiple of the acceleration of gravity 9 It is a unit free value and serves to compare the separation and sedimentation performance These values are calculated using the formula below 9 RPM RCF xrx1 118 RPM x 1000 1000 r x 1 118 RCF relative centrifugal force RPM rotational speed revolutions per minute r centrifugal radius in mm The relative centrifugal force RCF stands in relation to the revolutions per minute and the centrifugal radius 8 12 ROTOR IDENTIFICATION After every start of a centrifugation run the rotor utilised is identified After a rotor change the drive switches off and the rotor code rot xx is displayed Press The last used centrifuge data will be displayed A further operation of the centrifuge is only possible after a single opening of the lid If following a rotor change the maximum speed of the rotor is less than the set speed the speed is limited to the maximum speed of the rotor Instructions Cellspin 23 8 13 EMERGENCY RELEASE The lid cannot be opened during power failure An emergency release has to be executed by hand For emergenc
18. t time centrifugation The centrifugation run occurs while the key IMPULS is kept pressed Display the brake step and the centrifuging radius Instructions Cellspin 10 8 OPERATION OF CYTOCENTRIFUGE 8 1 OPENING THE LID Swing handle rail on the lid upwards The symbol L lid open illuminates in the rotation indicator Open the lid The lid can only be opened when the centrifuge is switched on and the rotor is at rest If it cannot be opened under these circumstances see the section on Emergency release 8 2 INSERTION AND REMOVAL OF ROTOR Removal of rotor Take the rotor out of the centrifuge by pulling at the centre holder of the rotor Place the rotor carefully onto the supplied bench top rotor holder Removal of Cellcliprotor out of the centrifuge Placement of Cellcliprotor onto the rotor holder Instructions Cellspin 11 Insertion of rotor Lift Cellcliorotor carefully from rotor holder Place Cellcliprotor centred into the centrifuge Ensure that the safety pin locks into holder at the centrifuge hub Safety pin on bottom side of the Cellcliprotor Instructions Cellspin Safety pin lock at the centrifuge hub Closed rotor Take the closed rotor out of the centrifuge by pulling at the centre holder of the rotor Unlock lid by pushing the lever on the lid to the centre and lift the lid Inside the rotor may develop a vacuum during centrifugation In this case hold the lower
19. tocentriftuge depends on how frequently the instrument is used Clean the centrifuge housing and the centrifuging chamber regularly using soap or a mild detergent and a damp cloth if required For one thing this services purposes of hygiene and it also prevents corrosion through adhering impurities In the event of condensation water formation dry the centrifugal chamber by wiping out with an absorbent cloth If infectious materials penetrates into the centrifugal chamber this is to be disinfected immediately Lightly rub the rubber seal of the centrituge chamber with talcum powder or a rubber care product after each cleaning Wear protective clothing and disposable gloves according to Good Laboratory Practices Please take note of the safety aspects of the instrument Do not use acetone or xylene for cleaning the unit Only use alcoholic media Never spray or use cleaning medium directly onto the touch panels Instructions Cellspin 28 10 2 RECOMMENDED MAINTENANCE AND SERVICE SCHEDULE Pull the mains plug before cleaning Before any other cleaning or decontamination process other than that recommended by the manufacturer Is applied the user has to check with the manufacturer that the planned process does not damage the device Centrifuge Clean the centrifuge housing and the centrifuging chamber regularly using soap or a mild detergent and a damp cloth required For one thing this services purpos
20. y release disconnect the centrifuge from the mains Open the lid only during rotor standstill Only the plastic release pin provided may be used for emergency release Switch off the mains switch switch position 0 Look through the window in the lid to be sure that the rotor has come to a standstill Insert the release pin horizontally into the hole Push the unlocking pin in until the handle can be lifted when the pin is pressed down Open the lid Instructions Cellspin 24 9 APPLICATION NOTES 9 1 APPLICATIONS Any cell suspensions may be processed with the Cellspin cytocentrituge Cerebrospinal fluids Pleural fluids Pericardial fluids Peritoneal fluids Synovial fluids Urine Bronchial alveolar lavage washes Oral cavity washes Gastric washes Exudates and transudates Fine needle aspirates and other aspirates Sputum 9 2 APPLICATION NOTES Sample Volume Cellfunnels are designed to give optimum results with sample volumes of 0 1 to 0 5 ml Double Cellfunnels are designed to give optimum results with sample volumes of 0 1 to 0 5 ml per chamber ECOtfunnels are designed give optimum results with up to 4 of sample Cell count To guarantee optimum results the cell count should be as indicated below Cellfunnel 100 000 or 10 cells per chamber Double Cellfunnel 100 000 or 10 cells per chamber ECOfunnel 1000 000 or 10 cells per chamber Samples with higher cell counts should be diluted
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