INSTRUCTION SERVICE MANUAL
Contents
1. opare parts parts necessary to the maintenance of equipment functions will be available for seven 7 years after production of the particular product is discontinued We therefore beg your indulgence and recommend that you be prepared for some situations in which certain repairs are impossible due to a lack of spare parts 4 9 Manufacturer requirements at o Repair or Maintenance We cannot repair or inspect a centrifuge if it has not been properly washed or sterilized beforehand or if it has been used in an RI facility or a facility operating at a level higher than P2 Please ensure that each machine has been properly washed and sterilized before offering it for repair or inspection 1 Required information when submitting an inquiry 1 Model PlateSpin 2 Serial number Ex F60015 See the name label at the left side panel of centrifuge 3 Trouble Provide a description of the trouble as detailed as possible 2 Required information that must be attached to the centrifuge in the form of a note 1 If you have pertinent information on your handling of biohazards fill out the items below and affix this information to the centrifuge 1 Environment where the centrifuge is used 3 Required measures against the biohazard 2 Please attach a Contaminant Elimination Certificate to the centrifuge gt Refer to Page 4 9 and 4 10 4 8 4 10 1 2 3 4 5 Section 4 Servic2. May 2007 Printed in Japan PlateSpin PLATE REFRIGERATED CENTRIFUGE INSTRUCTION AND SERVICE MANUAL ZN To ensure proper operation of the centrifuge be sure to read this manual carefully before operating it Also keep this manual handy so that you can refer to it at any time NOTE W The products being indicated in this Instruction Manual are designed for opera tors with expert knowledge and are intended only to be used by such qualified operators observing the indicated precautions for respective purposes For per sons lacking necessary expert knowledge these products may be difficult to use properly and may even pose a danger to use When the aforesaid persons lack ing the necessary expert knowledge are using these products do so under ap propriate supervision and guidance of a qualified operator possessing the neces sary expert knowledge W Do not distribute this manual within the U S A Mexico Canada and Australia as the products advertised in the manual shall not be distributed in these countries F or information added or modified after June 2007 please contact your local dealer KUBOTA CORPORATION 29 9 HONGO 3 CHOME BUNKYO KU TOKYO 113 0033 JAPAN 34 24910 WARRANTI Y Kubota Corporation Kubota warrants that the instrument covered by this warranty shall be free from defects in material and workmanship under normal use Kubota will repair or replace free of all charges the instrume
3. SPEED fF X100rpm cj 107x 10 g OOL When continuing on to time setting press the FUNCTION key The SPEED display stops flashing and the TIME display starts to flash Timer setting status SPEED amp TIME a 305 X min FUNCTION SJE ii S NU NOTE 6 The centrifuge can be started wth the speed on the SPEED display even if the TIME display of value is flashing e When you want to change the setting during the operation press the FUNCTION key 2 times on the control panel after changing the setting and the flashing of the display is stopped The operation becomes shifted to the setting that have been changed 6 The setting order of the speed and the timer can be changed gt Refer to page 3 16 Section 3 Operation 0 00 Setting the Timer OULU Pressing the FUNCTION key 2 times the TIME display flashes and indicates the current setting The timer is able to be set up 4 v CY mi FuNcTion E Imin XS P xO sec ILI E Pressing S PEED TIME knob changes the settable range of centrifuging time as des cribed below Set to the range as required Factory default the range is set minutes Setting minutes oettable range 2 Setting seconds oettable range 1 99 seconds 3 HOLD It will continuously operate regardless of the timer When set to hold both min and sec lamp will be turn off UUL Turning the SPEED TIME knob changes the indication on the display set to the ti
4. 2 3 4 5 of 5 kinds 0 Sound none SPEED TIME Y N x 100rpm O min C E o X10g Pos O sec 7 4 Then press the SPEED TIME knob while holding down the FUNCTION key The display returns to the previous Speed and Time indication after the setting Sound that informs the end of the operation is saved De 5 oection 3 Operation 4 Reverse of setting order of the Speed and the Timer The setting order of the speed and the timer can be reversed This function is useful when you often operate the centrifuge by changing the timer only Factory default 0 1 Pressing the SPEED TIME knob while holding down the FUNCTION key The used number of times of the centrifuge is displayed 2 Pressing the SPEED TIME knob several times and let indicate St on the TIME display The SPEED display indicates the current setting of the number see the figure below gt Refer to Page 3 12 Ex Numbers O SPEED TIME x 100rpm amp O min C LU o x10g E O sec V L o Speed RCF lTimer J Timer Speed RCF 3 Turn the SPEED TIME knob to change the number SPEED TIME x100rpm O min C f O x10g E O sec 4 Press the SPEED TIME knob while holding down the FUNCTION key The display returns to the previous Speed and Time indication after the setting Numbers is saved FUNCTION Q CH SX 3 16 Section 3 Operation UOU Setting the Reminder alarm The alarm reminds you every minu
5. 6 3 6 4 Troubleshooting 0 9 0 6 5 7 Caution Indicators EO 5 1 Alarm Indicators E1 5 2 E2 5 2 E3 0 2 E4 5 3 E6 5 3 E7 5 3 C1 0 4 C2 5 4 C3 5 4 C4 0 4 C5 90 4 Momograph for Centrifugal Force Calculation R RGF N Rotation Radius cm Centrifugal force X d Speed rpm 30 5000 c 50000 25 5000 500000 EE i 4000 400000 Lo 8000 so 30000 25000 15000 14000 13000 12000 n 1100 11000 CUBRE 1000 10000 z 60 amp X0 S07 500 00 9000 5 40 4 4000 30 700 d 7000 4 20 2000 Tr 1500 00 4 6000 10 1000 3 sna L 5000 How to use this Nomograph 1 You can get centrifugal force RCF with line connection between rotation radius cm and 2 You can get speed rpm with line connection between rotation radius cm and centrifugal Calculating Centrifugal force i r RGF 211 18 x XR 1000 RCF Centrifugal force g R Rotation Radius cm M Speed rpm Md i Hur PEAY VOS IEEECME ON SAQ ON 4009 uIdgeield PALETA WVeoVIG LINIJI ESI NS 00 9 NS e 1 4802 953 ZAN VV ADEZ 022 AIUO ZALON 18301 ta A IND jana o viov AGII OOl 10 0 A0 2 0c2 9 e o lag YOLVAND AR 5NIH LIAS AUEZ 22 9 ASII OOl 2N9 QN5 8 V Aad 2540 3118 agy I L moneen oan yal saloa Ts umema voso ru aoA SOSN3S v daads wemememu 2c fan sxfoaT muessa 20
6. 40 L A place with the ambient tempera ture beyond 40 E can introduce undesirable build up of heat inside the centrifuge and a place under 10 7 can cause the centrifuge malfunction and as the result ac cidents 1121 Installation 9 3 Water pipes City water pipes may not be adequate as a ground since it may consist of plastic piping Ensure that the ground cable is connected to the grounding termi nal This precaution must be strictly observed to avoid accidents due to electric shocks or leakage A clearance of 30 cm minimum must be provided around the cen trifuge If the centrifuge is driven into un controlled rotations due to a fail ure secondary damage can result from energy absorbed by the rota tion Do not install the centrifuge in a dusty place Do not install the centrifuge in a place with poor ventilation Otherwise inside temperature of the cen trifuge may rise resulting in accidents Do not install the centrifuge in a place with high humidity relative humidity 85 or above Leak or accident may occur N CAUTION adl Toxic or radioactive substances etc ee ex n When centrifuging of substan ces contaminated with patho genic bacteria or toxic or radio active substances always use containers that are pathogenic bacteria toxic substance or ra diation proof Otherwise infections intoxica tion or radioactive exposure ac cidents may occur i1 Fasten
7. SLOW ZaGcceleration DECO SLOWO SLOW deceleration Section 3 Operation ug Flashing operation The flashing operation continues as long as the FLASHING key is kept depressed The flashing operation decelerates and stops when the FLASHING key is released Operation 1 At first execute ordinary Operation 1 5 m thel 3 2 Operation gt Refer to page 3 1 Operation 2 Keep the FLASHING key depressed Operation 3 Operation 4 Operation 5 Operation 6 Operation 7 Lamp on the START key lights on F FLASHING and time of operation are indicated alternately on the TIME display Settable range 1 99 second Ex 10 seconds E O min O min YO f vo ava SEC aa SC As for the speed the rotor can be rotated at its maximum speed If the set value does not reach the maximum speed the rotor rotates only up to the set value As for the acceleration rate the rapid acceleration characteristics will be selected As for the deceleration strength the rapid deceleration characteristics will be selected gt Q FLASHING KUNA The operation will stop when you release the FLASHING key FLASHING and START lamp lights off The indication on the TIME display will show the value at the time when the FLASHING key was released until the lid will be opened Press the OPEN button to open the lid STOP key lamp remains lit Remove the sample If the centr
8. calculation result By setting the rotation radius an accurate speed and centrifugal force can be switched Factory default at the time of delivery is 100 mm P Refer to page 3 13 3 10 Allowable load and Reduced maximum speed AX WARNING Do not exceed the maximum speed and the allowable load of rotor and bucket An excessive speed or overload may cause a damage to the rotor bucket and the centrifuge If the load exceeds the limit reduce the maximum speed as shown in the formula 3 2 and set the actual speed below the reduced maximum speed formula 3 2 Reduced maximum speed rpm Maximum speed rpm X Allowable load gram Actual load gram 1 When the gravity of sample is 1 2 or higher or when a special tube is used check the allowable load of the rotor and bucket 2 Regarding allowable load on rotors and buckets at the maximum speed refer to the appropriate Specification Table for rotor in use 3 The allowable load includes the weight of sample cushions adaptors tubes caps and racks The weight of bucket is not included in the allowable load 3 19 Section 4 Service o oo Daily Inspection ZNWARNING f any abnormality is found during routine daily inspections discontinue centrifuge operation use the POWER switch to tum the machine OFF disconnect the power cable attach DO NOT USE labels to the rotor and the outside of the centrifuge and contact your nearest dealer to request a cen
9. or failure has been found occurring NOTE When operation centrifuge is interrupted by some abnormality failure or for repairs always turn off the POWER switch and attach DO NOT USE stickers to the rotor and the centrifuge before contacting your nearest dealer Section 6 Rotor g 00 Mounting RMP 62 Rotor When you purchase the centrifuge and rotor together the rotor will be in the state of being mounted on the centrifuge W hen replacing the rotor or when the rotor is removed for some reason refer to the mounting procedure in the manual for each rotor A CAUTION Make sure that the rotor yoke is firmly fastened to the drive shaft with the rotor locking nut Since the rotor is not fastened onto the shaft when these parts are loose excessive vibrations will occur and damage the equipment and the rotor may fly off inflicting phys ical injury ee 1 To install the rotor match the rotor groove with the pin of the drive shaft After mounting the rotor the screw is T type box wrench protruding 8 mm from the center hole of the rotor y 2 Fix the rotor by tightening the rotor locking nut Rotate the nut clockwise using the T type box wrench 10mm etc a Rotor locking nut Rotor yoke 3 After installation of the rotor hold the rotor yoke and attempt to move it up and down to ensure that there is no play r hole of rotor Drive pin X C
10. the lid ee 3 5 3 4 Setung the Speeds se 9se s sees 3 6 1 Setting the speed by therpm 3 6 2 Setting the speed by the centrifugal force x g a i i i a a Se page o5 Setting the TIME A s ee wes s ems 5 ae 3 6 Setting the acceleration deceleration e 0620606060006000600606009006009006060060600060999 3 9 3 7 Flashing operation 3 10 3 8 Memory Flashing operation 3 11 3 9 Setting the Function or 1 Setting the Rotation Radius 3 13 2 Setting the SLOW deceleration e c0606060600060060600606000060090060060909999 3 1 4 3 Setting the Sound that informs the end of the operation 3 15 4 Reverse of setting order of the Speed and the Timer 3 16 5 Setting the Reminder alarm 9 17 6 Setting the Indicators for the end of the operation 3 18 3 9 Calculating Centrifugal Force Dee by 3 10 Allowable load and Reduced maximum speed OH 1g Section 4 Service 4 1 Daily Inspections 9 eee 4 4 2 Monthly Inspections 4 2 4 3 Annual Inspection 4 2 4 4 Cleaning and Sterilization 4 3 1 Cleaning the chamber interior 4 9 2 Cleaning the rotor buckets and tube rack ee ee 4 4 3 Sterilization of rotor buckets and ubere were su Rr eer AU PEE 4 4 4 5 Greasingesssseeeeeennnnn nn nn 4 5 4 6 Inspection of Circuit Protector
11. 1150 V FESS ERR i ure 8 lt ni s wu weevrcyre 219060 V7 oe wemznsems 5 ni sn ss CERIA AWP D Arsa pue Aod sapag tD pem D ARIM nt sx wN agaaa Beu 1c Ies EZIOEO NJ Grra EN3 d vog LLO TW v50802 195 G0 GIOVMI G VIOX8ZM V8 AQES OdGG 8 LON MS NOOG es AS V8MI X HNIT OV Bid B SOLON I TBOPXS HH 418 na A85 N35 aaa GN aqq Autoclave record table Temperature of Number of times allowed Rotor RMP 62 Serial number 121 C exclusive use b Refer to page IV Number Tempera Time ture of times Se C minute a 03 00 a a sf 000 a a 00 a 00 a 0 a 000 ae 04 o0 a a a a a ae a ae a a a EN a
12. 12 Ex l Setting is the O rpm SPEED TIME x100rpm E O min X10 g M O sec OUOU Turn the SPEED TIME knob to set the natural deceleration speed Ex Setting the 300 rpm SPEED TIME K wv X 100rpm E O min O x10 g f7 o sec J OULU Press the SPEED TIME knob while holding down the FUNCTION key The display returns to the previous Speed and Time indication after the setting Natural deceleration speed is saved EB D lu oection 3 Operation 3 Setting the Sound that informs the end of the operation The Sound that informs the end of the operation can be selected from the 5 kinds Sound none Use this function to identify each Sound that informs the end of the operation when you use more than one centrifuges Factory default 1 1 Pressing the SPEED TIME knob while holding down the FUNCTION key The used number of times of the centrifuge is displayed 2 Pressing the SPEED TIME knob several times and let indicate bu on the TIME display The SPEED display indicates the current setting of the sound that informs gt Refer to Page 3 12 Ex The Sound that informs the end of the operation 1 SPEED TIME f x 100rpm O min C Oo x10g 5 Lj O sec X 3 Turn the SPEED TIME knob to change the Sound that informs the end of the opera tion Pressing the FUNCTION key makes the selected buzzer sound once The Sound that informs the end of the operation can be selected from 1
13. 198 V to 253 V Table 2 1 Rated Voltage Current and Acceptable Voltage Range and Current Requirement AX WARNING The power cable alone should be connected to the plug socket Do not use a branched plug socket which may cause overheating or fire section 2 Installation and Power Supply 2 5 Grounding NOTE When using the centrifuge for the first time plug the power cable on the centrifuge into the inlet on the back of the centrifuge ZNWARNING Ensure that the ground wire is connected to the grounding terminal ZNWARNING Do not connect the ground cable to the following places 1 Gas piping Explosion or fire may occur 2 Ground cable of the lightning conductor or telephone cable Electric shock may occur in case of thunderbolt 3 Water pipes City water pipes may not be adequate as a ground since it may be connected to plastic pipework Knife switch water pipes Gas piping Plug Socket Power plug Ground wire Green and yellow stripe Ground terminal pattern Section 3 Operation ug Cautions of Operation e When using this centrifuge observe the contents of the Section General Notes being described in the front part of this document and the precautions given in respective sections 1 00 Operation Operation Operation Operation Operation Operation Operation Operation 1 Tum on the POWER switch After the STOP lamp on the control panel
14. 4 6 4 7 Using the Photoelectric Tachometer Port ee 2 2 4 7 page OL Spare Parts Supply TTTTTTTITITIT 8 UU Manufacturer requirements at Repair or Maintenancd TILILLILILILLL 4 9 LOL Product Preparation When Returning Units for Repair or for Other Reasons TOT 9 Contaminant Elimination Certificate ELEETTTTTTTTTTTTTTTTTITITITI 4 30 Section 5 Troubleshooting O0 7L U Caution Indicator OOO 9 4 EO TITITITTITTTTTTITITUTITITITI gt 4 0 00 Alarm Indicator O gt 2 E1 TITITTITITITITITTITITITITITI gt 2 E2 TITITTITTTITITITTITITITITIT gt 2 E3S TITITIITITITITITTITITITITIT gt 2 E4 Tn gt 3 Ee mmm gt 3 E7T TIT gt 3 CA TITTTITTITTITTTTTITITITITITI gt 4 C2LTITTTTTTITTITTTTTITITITITITI gt 4 CoC TTA TTT moh gt 4 CA TITTTITTITTITTTITITITITITITI gt 4 CS TITITITTITTITITITITITITITITI gt 4 UU Troubleshooting TTITTTTLDILTLITIITI 9 9 Section 6 Rotor 0 00 Mounting RMP 62 Rotot 0O10 6 4 0 00 RMP 62 Swinging Bucket Rotor TT1 6 2 OO0 Specification LTITITITITITUITITIITI 9 2 IL Distribution ofsamp les OOOO 6 2 OOU Setting plate 1O 6 3 UE Allowable weight of micro plate JI 6 4 page Section 7 Specifications L LI Centrifuge TTTTTITTITTITTITITITIT 7 1 CL L Standard Accessories TTITITITIITI 7 4 Section 9 Parts List 7L Recommended Spare Parts III UU 9 1 Index General Notes Be sure to follow them oince large electrical and mechanical energie
15. 4 ways of switching Lid open Imbalance Over speed Function for detecting an Alarm display occurrence of electrical abnormality in speed sensor invertor Lid rock Motor opeed setting Digital display 200 2 500 rpm in 100 rpm steps Speed indication Digital display 0 2 700 mm in 100 rpm steps Digital display in 100 x g steps RCF setting 100 700 x g The centrifugal force differs by the rotation radius oe Digital display in 100 x g steps RCP indication 0 820 x g The centrifugal force differs by the rotation radius From 1second to 99 seconds 1 second step Timer setting range 1 minutes to 99 minutes 1 minutes step HOLD Setting the Sound that informs the end of the 5 kinds Sound none operation Rated voltage and Rated 110 115 V 50 60 Hz 1 3A current 220 230 V 50 60 Hz 0 6A Totslissnt Reden 110 115 V 60W 220 kJ h Pat ee uice 220 230 V 70 W 252 kJ h Dimensions Weight 35 W x 42 D x 32 H cm 26 kg oafety regulation IEC 61010 2 020 CLASS Temperature 10 to 40 C Operation environment Atmospheric pressure 70 to 106 kpa 700 to 1 060 mbar Humidity 30 to 8596 1 00 Standard Accessories 1 Instruction manual Copy 2 Power cabe 1 Pc 3 RMP 62 Roto p Pe 4 Rotor locking nu E 1 Pc 5 Grease No 067 0040YJ MMII 1 Pc Section8 Dispose of the centrifuge unit rotor and accessories Dispose of the centrifuge unit rotor and accessories according to local practi
16. C key STOP key To change for ACC and DEC Press this key to discontinue operation Refer to Page 3 9 p d FUNCTION key P Refer to Page 3 2 To change for speed RCF and time FLASHING key While pushing the key the rotor operates in the rapid acceleration and the rapid deceleration characteristic P Refer to page 3 10 Section2 Installation and Power Supply o oo Unpacking When the centrifuge is taken out of a corrugated carton box check the following OUL Upon receiving the centrifuge examine it for any visible damage caused during transportation If any is found contact the dealer immediately LI LI I Confirm that all the accessories listed in 7 2 Standard Accessories are included with the delivery P Refer to page 7 1 1 00 Place of installation NWARNING A clearance of 30 cm minimum must be provided around the centrifuge If the centrifuge is driven into uncontrolled rotations due to a failure secondary damage can result from energy absorbed by the rotation ZNCAUTION Do not install the centrifuge on an inclined slippery or unstable surface Violent vibration may occur When installing this centrifuge keep 3meters or more away from AM radio Noise generated by this centrifuge causes the sound of AM radio deteriorated ng Movement of centrifuge ZNWARNING Never move the centrifuge while the rotor is rotating or while the rotor is attached to the centrifuge O
17. HING key once again in case that you want to check the operation time during the flashing operation e When you want to halt operation Press the STOP key Operation 3 When the equipment reaches the flashing time which was last memorized the operation will be decelerated to come to a complete stop Then the START key lamp will turn off and the STOP key lamp will flashing The last memorized FLASHING time will appear on the TIME display Operation 4 Press the OPEN button to open the lid STOP key lamp remains lit Operation 5 Remove the sample Operation 6 If the centrifuge is to be used again return to Operation 4 in the 3 2 Operation p Refer to page 3 1 Operation 7 After finishing use of the centrifuge turn OFF the POWER switch to tum off the power supply LL Section 3 Operation 1 gg Setting the Function As for this product the following setting 1s produced Choose the item you want to set up and press SPEED TIME knob The page where the content is written is referred to 10 O Pressing the SPEED TIME knob while holding Funcrio down the FUNCTION key 7 Y SPEED TIME x100rpm 85 O min Oo x10 g O sec SPEED TIME x 100rpm DOG O min O x10 g O sec SPEED TIME x100rpm B O min o X10 g b O sec OU U The used number of times of the centrifuge is displayed Ex 1186 times L OULU Setting the Rotation Radius P Refer to Page 3 13 Ex 100 mm M
18. LI LI U Setting the SLOW deceleration gt Refer to Page 3 14 Ex L 300rpm w SPEED IM 1 U Setting the Sound that informs x100rpm the end of the operation f Usus Ea P Refer to Page 3 15 Ex 1 SPEED TIME LI LI L Reverse of setting order of the X100rpm 5E O min Speed and the Timer fF 2X40 a sec gt Refer to Page 3 16 Ex 0 SPEED TIME OU L Setting the Reminder alarm 100rpm i RR min gt Refer to Page 3 17 li o x10 o Geet Ex ON LI LI L Setting the Indicators for the SPEED LME end ofthe operation X 100rpm B P O min gt Refer to Page 3 18 o x10 g Drac Ex 1 00000020 W After setting any one of the above please return the display to the previous indication of SPEED and TIME To do above press the SPEED TIME knob while holding down the FUNCTION key Section 3 Operation MMU Setting the Rotation Radius To obtain more accurate centrifugal force adjust the rotation radius to that of the rotor buckets Factory default 100 mmll MML Pressing the SPEED TIME knob while holding Function Cd down the FUNCTION key Q X The used number of times of the centrifuge is displayed OLU U Pressing the SPEED TIME knob several times and let indicate r on the left side of the SPEED display The SP EED and TIME display indicates the current setting Unit mm gt Refer to Page 3 12 Ex The rotation radius is 100 mm SPEED TIME f X 100mm f O x10
19. OOOSgNSBSESB ovDogpygeymum QOOOQOQOO OOOOOOOO OOOOOO0O00 OOOOOOO0 OO0OOO0000 QOOOQOQOO QOOOOQOCO OULU Setting plate A CAUTION Use the same plates The wrong arrangement will cause imbalance and resulting in damage to rotor bucket or the centrifuge If a plate is difficult to remove Plate with the sample Place an extra empty plate in the bucket and place the plate with samples on top of it This will facilitate later re moval of the plate with samples Bucket Section 6 Rotor 4 Allowable weight of micro plate NWARNING W When pile up the micro plate to each bucket use it after the sufficient test for the strength of the plate is made The more the micro plates are piled up the easier the plate at the lower position becomes damaged ll Allowable weight of micro plate and cell culture plate put in the bucket is 300 gram If it exceeds 300 gram rectify reduced maximum speed by the following formula If it is not used at the rectified speed the rotor and the bucket will be damaged causing the accident Reduced maximum E i Allowable load gram speed rpm P a Actual load gram Section 7 Specifications 0 00 Centrifuge Max speed 2 500 rpm Max RCF 700 x g Max Capacity Deep well plate 2 Micro plate 6 Invertor Microprocessor control Speed Centrifugal force time Control system acceleration deceleration Acceleration Deceleration Rapid Slow
20. a rotor db i 41 Bucket r5 Tube o Ensure that the rotor is firmly fastened to the drive shaft If not positively held in place the rotor or centrifuge can be damaged thereby causing acci dents The same type buckets must be provided to every rotor yoke If not positively held in place the rotor or centrifuge can be dam aged thereby causing accidents Use the same type of tubes The wrong arrangement will cause imbalance and resulting in damage to rotor bucket or the centrifuge amp 1 Balance of sample o Keep the load of the sample bucket etc balanced If an appropriate balance is not provided unexpected accidents can result from a damaged rotor or centrifuge 17 Cleaning Do not use detergents exceed ing the range of pH 5 8 or chlor ine detergents for washing pur poses Corrosion may damage the rotor and bucket resulting in damage to the centrifuge 41 Caution plate 9 Do not remove the caution plates When a caution plate becomes dirty blurred or peeled off re place it with a new one caution plates are available at charge Usable Rotor NWARNING OOL The rotors that can mount as of May 2007 RMP 62 is exclusively used for Model PlateSpin gogo Do not use any rotors other than RMP 62 If rotors other than RMP 62 is used the rotors may be broken resulting in a serious accident OOU This information is subject to addition or change For info
21. below OOU When any of the alarm indicators turn on during operation when the rotor is spin ning the rotor immediately decelerates and stop s Control panel NOTE Ex TIME display TIME O min O sec The equipment is inoperative while any of the alarm indicators is turned on Imba lance alarm This indicator flashes and the rotor stops rotation when excessive vibration is detected Balance the load samples or bucket 2 Remove dust and dirt from the sliding surface and apply grease so that the bucket on the swing rotor swings up smoothly Contact your local dealer for inspection when this alarm continues to flash even after taking the above measures Lid open alarm This indicator lights when the lid is open Close the lid completely When the alarm recurs contact your nearest dealer for inspection Inverter alarm This indicator flashes when abnormal signal comes out from the inverter After the rotor stops spinning turn off power supply once and turn on the power after about 30 seconds When the alarm recurs contact your nearest dealer for inspection Speed se nsor alarm This indicator flashes when the self inspection function detects the fault or abnormality in the speed sensor section 5 Troubleshooting After the rotor stops spmning turn off power supply once and turn on the power after about 30 seconds When the alarm recurs contact your nearest deale
22. careful to maintain lubrication by ensuring that a proper amount of grease is present in contact section between the rotor yoke shaft and the bucket section A in the schematic diagram below A lack of grease may allow violent vibration to occur resulting in damage to the centrifuge NOTE 1 If the buckets do not swing up smoothly as the rotor is spun up to speed apply the attached grease lightly on the contact section between the rotor yoke shaft and the bucket to the section A shown in sketch below Move the bucket from side to side and up and down so that the grease may be spread evenly 2 Wipe off any dirt or congealed material before applying fresh grease using alcohol or acetone 3 If the buckets do not swing up properly after applying the grease contact your local dealer for an inspection 4 When the provided grease is used up purchase replacement grease of code No 067 0040 from your nearest local dealer If the buckets do not swing up smoothly as the rotor is spun up to speed apply the attached grease lightly on the contact section between the rotor yoke shaft and the bucket Move the bucket from side to side and up and down so that the grease may be spread evenly Rotor yoke shaft Bucket Section 4 Service AO Inspection of Circuit Protector The circuit protector is automatically activated when the electric circuit failure or overcurrent occur In such a case the white portion of the circuit prot
23. ces for the di sposal of industrial waste If you encounter problems concerning proper disposal contact your nearest dealer If the centrifuge unit rotor or accessories to be discarded are contaminated with radioactive explosive toxic or infectious substances that may be harmful to people s health you must notify the waste handlers concerning the details of such contaminants Section 9 Parts List 0 00 Recommended Spare Parts Index A Acceleration Deceleration 1 2 3 9 ACCEL 1 2 3 9 Allowable load 3 19 Appearance 1 1 Autoclaving V C Calculating Centrifugal Force 3 19 Centrifugal force xg 3 7 Circuit Protector 4 6 Cleaning and Sterilization 4 3 4 4 Contaminant Elimination 4 9 4 10 Control Panel 1 71 1 2 D DECEL 1 2 3 9 3 12 3 14 F Flashing 3 10 3 11 G Greasing 4 5 Grounding 2 3 H HOLD 3 8 Indicators for the end of the operation 3 12 3 18 Inspection 4 1 4 2 4 8 Installation 2 Lifetime of rotors IV min 3 8 Movement of centrifuge 2 1 Natural deceleration 3 12 3 14 Opening and Closing the lid 3 4 3 5 Operation 3 1 9 2 3 3 Power Requirement 2 2 Reduced maximum speed 3 19 Reminder alarm 3712 3 17 Reverse of setting order of the Speed and the Timer 3 12 3 16 Rotation Radius 3 12 3 13 Sec 3 8 Sound that informs the end of the operation 3 12 3 15 opeed 3 6 START 1 2 3 2 STOP 1 2 3 2 T Tachometer port 1 1 4 7 Timer 3 8 U Usable rotor IV Rotor RMP 62 6 1 6 2
24. e Product Preparation When Returning Units for Repair or for Other Reasons Remove any contaminants from units or items that will be returned for servicing Prior to sending back our products including accessories for repair or for other reasons be sure to remove any and all contaminants Attach a Contaminant Elimination Certificate To eliminate possibly harmful contaminants the user must take appropriate action on his or her initiative Fill out a Contaminant Elimination Certificate as shown on page 4 11 and attach it to the item to be returned Caution Contaminant means radioactive materials poisonous materials contagious pathogens and so on The preparatory procedure to eliminate all traces of such contaminants therefore varies depending on the respective materials Please take appropriate action to prevent any possible hazard to personnel who will be handing the machine equipment or parts Equipment contamination elimination goal The elimination of contaminants from the equipment is aimed to safeguard the persons who will inspect and repair the returned products at our facilities If a Contaminant Elimination Certificate is not attached If a Contaminant Elimination Certificate is not attached to the returned products we receive we will contact the person in charge to confirm the details If we cannot confirm that potentially hazardous contaminants have been properly eliminated and we find that we cann
25. e the centrifuge for separation of any hazardous material explosive chemically active organic or radiation containing material or material polluted by pathogenic microorganisms and Oil Copyright 1995 KUBOTA Manufacturing Corporation oafety Instructions The centrifuge and the manual indicate important information in order to ensure safe operation of the centrifuge and to prevent physical injuries and property damages Be sure to understand the meaninas of the followina indications and follow the instructions 1 Explanation of indication marks Indication W ARN IN G t is a possibility of Serious accident resulting in ii death or serious injury It is a possibility of accident resulting in slight or AN CAU TI Q N non fatal injury or property damage W Serious injury is defined as injuries such as loss of eyesight burn high low temperature electric shock bone fracture poisoning causing aftereffects or any other injunes requiring long term medical treatment at hospital W Non fatal injury is defined as bums electric shock or any other injuries which do not require long term medical treatment at hospital Property damage is defined as expansion damage related to damage to equipment or other property 2 Explanation of pictorial marks Pictorial marks Indicates prohibition things you must not do Details are shown near the mark using illustration or sentences Indicates requirements things you ha
26. ector located at the bottom left on the rear side of the centrifuge will stick out Circuit protector Recovery procedure is as follows 1 Turn off the power switch 2 After the rotor stops completely open the lid and check the rotor following the procedure given in 3 3 Opening and Closing the lid 2 Opening the lid during power failure on Page 3 4 3 Close the lid and then push the sticking out white portion until it becomes unseen 4 Turn on the power switch 5 Re start operation and see if it works If the circuit protector 1s activated again contact your local dealer Section 4 Service 4 7 Using the Photoelectric Tachometer Port A tachometer port transparent window is provided at the top of the lid Through the tachometer port the actual rate of rotation can be measured using a photoelectric tachometer 1 When using a photoelectric tachometer place a piece of reflective tape 5 20mm away from the center of the rotor 2 Mount the rotor on the drive shaft gt Refer to Section 6 3 After turning the power ON set the photoelectric tachometer so that its tip is right above the tachometer port on the lid The photoelectric tachometer can then measure the actual rotation rate Photoelectric tachometer Tachometer port Lid Poon Centrifuge Do not apply force to the tachometer port window from inside the lid Section 4 Service 4 8 Spare Parts Supply NOTE
27. enter axis of rotor X Screw 4 Penn Drive shaft Groove Drive pin 9 Do not start the operation when the rotor locking nut is removed When the drive pin is not aligned with the pinhole of the rotor bottom or the rotor is incompletely fastened to the drive shaft the screw is protruding only 3mm from the center hole of the rotor In such a case re install the rotor Section 6 Rotor 0 dd RMP 62 Swinging Bucket Rotor OOU Specification Calculating Centrifugal Force for RMP 62 Because of the size of the micro plate the radius of rotation differs between the center and the both ends with respect to the rotation axis resulting in the difference in the centrifugal force The centrifugal force of the place A cm Distant from the center of a bucket can be calculated in accordance with the formula where the rotation radius R shall be substituted with the Rx to be obtained by the formula mentioned below 4 emD V Of emt O O emi where Rj Radius rotation Minimum em Rk Actual rotation radius in a position which is A cm away from the center of the bucket Number Number Alowable Radius Allowable of of Plate Plate Sizes Speed R Re load wells Plate Mate nal mm rpm Buckets cm gram 2 T9 Micro plate HU D a Bucket is zs gt qiio gum 000 Deed ooooon om LE oo PCR plate rotor oo X BED x 00 1 The maximum speed drops when the step number increas
28. eral times and let indicate bL on the TIME display The SPEED display indicates the current setting of the number see the figure below gt Refer to Page 3 12 Ex Number 1 SPEED TIME p X100rpm i O min O X10 g O sec SPEED and TIME display After Setting value lights Remained period of the operation lights D UU OO UL flashing UU LLL DD flashing OOL Turn the SPEED TIME knob to change the number SPEED TIME WON 2 X100rpm f a O min O x10 g O sec OO Press the SPEED TIME knob while holding down the FUNCTION key The display returns to the previous Speed and Time indication after the setting Numbers is saved FU NOTION P CF V H After Setting value flashing Remained period of the operation lights oection 3 Operation 3 9 Calculating Centrifugal Force You can readily calculate the centrifugal force by assigning the Rotation Radius and speed in the following equation formula 3 1 Speed N rpm V2 Centrifugal force RCF X g 11 18 X B X Rotation Radius R cm Please refer to the Nomograph for Centrifugal Force Calculation at the last page of this manual NOTE As for rotation radius please refer the page 6 2 The maximum centrifugal force being indicated in the said pages is calculate by substituting the maximum radius of gyration of the rotor for the radius of gyration of each rotor cm and by rounding the first digit above the decimal point of the
29. es although a micro plate is possible to piling 3 steps When pile up the micro plate to each bucket use it after the sufficient test for the strength of the plate is made The more the micro plates are piled up the easier the plate at the lower position becomes damaged Refer to Page 6 4 2 PL Plastic 3 The left side is the RCF of radius R 1 The right side is the RCF of radius R2 4 The value of the RCF thatis indicated to the display is showing the value that cut the lower 2 digits Refer to Page 3 13 5 Allowable load per bucket This load includes the weight of the sample plate etc The weight of bucket is not included in the allowable load NOTE Never use any user modified rotors or buckets or adapters other than those specified by us including adapters made by unauthorized users We shall in no event be liable for any accidents or trouble incurred due to the use of such unauthorized parts Section 6 Rotor 00 Distribution of samples S CAUTION mi Keep the load of the sample balanced If an appropriate balance is not provided unexpected accidents can result from a damaged rotor or centrifuge ill Keep the load balanced symmetrically within 5 gram Correct Wrong 660000000000 660000000000 O00 000 OOO g ooo O00 m 000 OOOMN ooo O00 T COO O00 000 OO0 gm 000 ooosM ooo OOONMNM COO Sample OOoOOM f OoOopepatm OCOMPRAS
30. fuged Wipe the dirt off using a cloth containing a small quantity of neutral detergent 2 If rust is present Superficial rust can be removed by using a mild solution of sodium bicarbonate NaHCO3 bicarbonate of soda baking soda 1 Moisten a paper towel with a little water apply some bicarbonate of soda and then rub the area where rusting has occurred 2 After removing the rust with the bicarbonate of soda follow the above procedure 1 and clean the chamber interior using a neutral detergent followed by a thorough wiping with plain water Make sure that such cleaning is especially thorough so that all traces of the bicarbonate of soda are removed NOTE Under no circumstances should any rust inside the chamber be removed by using sandpaper or abrasive agents since if the interior surfaces are scratched this will only increase the likelihood of additional rust developing Cont d on next page Section 4 Service ONU Cleaning the rotor buckets and tube rack ZNCAUTION Do not use detergents exceeding a pH range of 5 8 or chlorinated detergents normally used for washing Corrosion may damage the rotor bucket or tube rack resulting in damage to the centrifuge that may lead to serious additional damage or accidents involving physical injury NOTE Do not allow any spilt samples to remain on any surfaces otherwise rust or corrosion may occur Also if sample spillage is left betwee
31. g 3 digits 2 digits 1 digit LL U Tum the SPEED TIME knob to set to the rotation radius of the rotor bucket to be used for operation The settable range is from 50 to 120 mm SPEED TIME WN j x 100rpm O min Q i o x10g O sec 2 digits 1 digit IL Pressthe SPEED TIME knob while holding down the FUNCTION key The display returns to the previous Speed and Time indication after the setting rotation radius is saved FUNCTI ON Q DU SE The rotation radius can not be stored in the memory when the power supply is tumed off without retuming the display to the previous NOTE indication Section 3 Operation UOU Setting the SLOW deceleration You can set the natural deceleration starting speed N by the following method Factory default it is set as O pml The setting range of N is from O to 2 500 rpm 1HFHTI FI 1 000 rpm or more LTTT1T1 11 1 000 rpm or less sper RAPID speed RAPID SLOW The deceleration characteristics changes to the SLOW from RAPID at 1 000 mm LUO Natural deceleration TET Natural deceleration TIME TIME LI IL Pressing the SPEED TIME knob while holding down the FUNCTION key The used number of times of the centrifuge is displayed OULU Pressing the SPEED TIME knob several times and let indicate Fr on the TIME display The SPEED display indicates the current setting of the natural deceleration speed Unit rpm gt Refer to Page 3
32. g disconnected 9 The STOP key from the power outlet lamp does not lit even if the POWER switch is turned on Is power supplied to the socket or knife switch Isthe power supply voltage correct Notel The power is turned off imme diately even if the POWER switch is turned on Isthe power supply voltage correct Notel Isthe POWER switch turn on Isthe STOP lamp lit or flashing The lid does not open even if the OPEN button 1s pushed Does it open when the power switch 1s turned off and turned on once again in 10 seconds Can you push the OPEN button smoothly Is the P OW ER switch turn on Is the lt STOP gt lamp lit The motor does not spin the alarm El E2 EA E6 E7 gt flashing on n The speed does not reach the set value even if the knob is Isthe power supply voltage low Note 1 turned Note 1 Read 2 4 Power Requirements on page 2 1 Note 2 Read 3 3 Opening and Closing the lid on page 3 4 Insert the power plug into the socket Inspect the power supply Use a power supply in the correct voltage range Use a power supply in the correct voltage range Pull up the POWER switch to turn the power on Waituntil the rotor is completely stopped Check if an imperfect contact is not occurring with the power cable connection After open the lid with pulling the string of the lid extreme release and collect the sample a
33. he power remove the 2 screws on the left and the right sides of the centrifuge 4 Insert a minus driver into the clearance between the front cover and the centrifuge and turn the front cover over gently to the front side 5 Press the OPEN button while holding down the lock plate The lid opens after the lid lock has been released NI Lock plate c t 4 Push OPEN button String Connection stick When the string is Cut it can be fixed by The inside of the centrifuge tying the ends 3 Closing the lid Close the lid by pressing the front center of the lid by hands Press the lid securely and lock it NOTE The lid can be closed even when there is a power failure or the power is turned off Section 3 Operation 1 BD Setting the Speed UULU Setting the speed by the rpm OU Press the FUNCTION key The SPEED display flashes and indicates the current setting ASPE Di x100rpm ir hs X10 g J OU U Check if thel X 10 al lamp tumed off when the X 10 all lamp is off OOOOH Proceed to the Procedur LI LI SPEED CTIO C Z s Ro 7 Xx 100rpm in O O X10 g 2 When thd X 10 gl lamp is od MNNM Press the SPEED TIME knob and the X 10 gl lamp turns off Proceed to the Procedur U U SPEED x100rpm D 10 a OU Tum the SPEED TIME knob to set to required speed The speed can be set 100 rpm intervals A SPEED fi x 100
34. id when the rotor is spinning Physical contact with the spinning rotor or bucket may cause serious injury 7 Stenlization o Do not perform dry heat or auto clave sterilization with tempera tures higher than those specified Otherwise the rotor may deterio rate and cause an accident t B Rotor and drive shaft during rotation gt Do not touch rotor and drive shaft during the rotation Physical contact with the spinning rotor or drive shaft may cause serious injury 2 Damaged corroded rusted or deformed Discontinue use of the equipment when its rotor or the buckets found to have been damaged corroded rusted or deformed Otherwise failure may occur Contd on nex oage ANWARNING 1 0 o Lifetime of rotors Use of rotors beyond the lifetime may lead to breakage of the rotor If the rotor is used continuously even after the lifetime of the rotor has expired should the rotor get damaged an accident may occur 11 Grounding Do not connect the ground cable to the following places 1 Gas piping Explosion or fire may occur 2 Ground cable of lightning Conductor or telephone cable Electric shock may occur in the case of lightening N CAUTION 1 Installation O Do not install the centrifuge on an inclined slippery or unstable sur face Violent vibration may occur Do not install the centrifuge in a place where the temperature is below 10 or over
35. ifuge is to be used again return to Operation 4 in the 3 2 Operation gt Refer to page 3 1 After finishing use of the centrifuge turn OFF the POWER switch to turn off the power supply Section 3 Operation 0 00 Memory Flashing operation The last ordinary flashing operation is memorized automatically When the FLASHING key is pressed shortly within a second the memory is called out and the equipment will make spinning down for the same time as the last operation It is not necessary to keep depressing of the flashing key This function is convenient when it is desired to maintain the spinning down condition Operation 1 At first execute ordinary flashing operation gt Refer to page 3 10 Operation 2 When making the 2nd flashing operation press the FLASHING key shortly within a second If you keep it depressed for more than a second the equipment will make ordinary flashing operation Lamp on the FLASHING key and START key light tum on F FLAS HING and time of operation are indicated alternately on the TIME display settable range 1 99 second Ex Ca 10 seconds e O min En O min A 1g sec sec As for the acceleration rate the rapid acceleration characteristics will be selected As for the deceleration strength the rapid deceleration characteristics will be selected Release the key within a second Mesin M ro Ie EN FLASHING gt KUZOA NOTE Press the FLAS
36. itch to turn the power on The POWER switch locates on the front side panel of the centrifuge 2 The STOP lamp on the control panel will be lit 3 Pressing the OPEN button the lid can be opened NOTE The lid is locked in following cases and will not open even if the OPEN button is pressed When the rotor is spinning When there is a power failure or the power is turned off OPEN button 2 Opening the lid during power failure ZNWARNING Do not open the lid while the rotor is spinning When a power failure occurs during operation of the centrifuge the rotor will naturally decelerate to stop its rotation If the lid is unlocked forcibly before the rotor stops the person can be entangled in the rotor resulting In a serious injury or death 1 Turn off the POWER switch Push 2 By using a flat blade screwdriver remove the lid extreme release cap on the lower part of the left OPEN side of the unit re button While pulling the string straight push OPEN py button Then the lid opens Lid extreme release cap x Do not pull the string too strong section 3 Operation If the lid extreme release cap string is cut WARNING For prevention of electric shock wait at least 5 minutes before opening the front cover 1 Turn off the POWER switch 2 Remove the power supply plug from the outlet 3 When more than 5 minutes have passed after turning off t
37. lights press the OP EN button Mount the rotor on the drive shaft P Refer to Section 6 Place the sample in the buckets gt Refer to Section 6 NOTE Close the lid firmly If the lid is not closed properly the centrifuge is not able to start Make sure the lid is closed firmly Proceed to operation 8 when operating with the same setting values as before set the respective parameters Set to required speed P Refer to page 3 6 Set to required time P Refer to page 3 8 Set braking force during deceleration and acceleration speed with the ACC DEC key gt Refer to page 3 9 Refer to page 3 10 to carry out Flashing operation NOTE Press the SPEED TIME knob to check the centrifugal force of the set speed or speed of the set centrifugal force Cont d on next page oection 3 Operation Operation 8 Press the START key Pip sound and lamp on the START key lights Value on the TIME display starts decrea ASPR sing Himin Lj T When the value becomes O the centrifuge automatically decelerates and stops Operation 9 In the following case 1 When you want to change the setting speed RCF time during the operation Press the FUNCTION key 1 or 2 times on the control panel after changing the setting and the flashing of the display is stopped The operation becomes shifted to the setting that have been changed FUNCTION 2 When you want to halt o
38. me required OUL Press START key on the control panel The operation will be started When started the display will diminish and stop at point 0 NOTE he centrifuge can be started with the time on the TIME display even if the display of value is flashing e When you want to change the setting during the operation press the FUNCTION key on the control panel after changing the setting and the flashing of the display is stopped The operation becomes shifted to the setting that have been changed Ihe speed and the timer can be set in this sequence or vice versa As for the change of the setting please refer to page 3 16 Section 3 Operation 1 00 Setting the acceleration deceleration When blowing up of samples is feared adjust the acceleration and the deceleration setting by pressing the ACC DEC key SLOW RAPID acceleration acceleration The acceleration characteristics changes to the RAPID from SLOW at 1 000 rom E Ex SPEED f OOU rpm p TIME Natural deceleration RAPID SLOW deceleration deceleration NOTE You can set the natural deceleration starting speed N P Refer to page 3 14 RARCC ACC RAPID RAPID acceleration J DEC DECI RAPID RAPID deceleration VS ACC ACCO RAPIDO RAPID acceleration WA Ec DECO SLOWLI SLOW deceleration M On V Y 4 Off 2 AcCHU sLOWI SLOW acceleration M S DECO RAPIDO RAPID deceleration l ACC DER ACC SLOW
39. n the rotor and shaft later detachment of the rotor may become problematic 1 If sample spillage has occurred remove the rotor buckets and tube rack from the centrifuge and wash the affected items with a neutral detergent and warm water Then rinse the items with the distilled water and thoroughly dry them before use 2 If water has accumulated inside the rotor place the rotor with its bottom side up and allow it to dry completely 3 If a sample has spilled onto the drive shaft wipe it off using a cloth moistened with a small amount of a neutral detergent and then clean away all detergent traces using a cloth moistened with water Then dry the surfaces completely before using the machine OULU Sterilization of rotor buckets and tube rack ZNWARNING Do not heat the rotor buckets or tube rack above 121 C for sterilization or disinfection purposes Also do not use an autoclave for dry heat sterilization since excessive heat may deleteriously affect the strength of the rotor buckets or tube rack resulting in breakage of the rotor buckets or tube rack causing serious damage or accidents involving physical injury Yet You can conduct up to 50 times of autoclave sterilizations of the RMP 62 rotor and bucket at 121 C gt Refer to poaae V To disinfect the rotor buckets or tube rack a 70 ethanol solution or ultraviolet radiation 1s recommended Section 4 Service 4 5 Greasing CAUTION Be
40. nd then contact your local dealer for inspection Pull up the POWER switch to turn the power on Go back to Problem 1 2 and check the power supply gt Refer to pages 5 2 and 5 3 Isthe lid closing Note 2 Close the lid firmly Use a power supply in the correct voltage range Cont d on next page oection 5 Troubleshooting Distribute buckets and tubes Are buckets and tubes correctly correctly When the error recurs distributed Note 3 contact your nearest dealer for inspection ee Do the buckets swing smoothly apray M Oa SUDO 6 Excessive vibration trunnion pins or the error El lit Are the rotor locking nut loose Fasten the rotor locking nut Note 3 securely Fasten the rotor locking nut Check if the rotor is not loose securely Are buckets and tubes correctly Distribute buckets and tubes distributed Note 3 correctly 7 An abnormal noise sounds Spray lubricant to buckets and Do the buckets swing smoothly trunnion pins Note 3 Read section 6 Rotor on page 6 1 If the error still remains even after the troubleshooting is made and the action has been taken or in case the following phenomena are occurring stop the operation Q The rotor or metal tubes has been found to be damaged or corroded A burnt smell comes out of the equipment You receive a minor electrification when you touch the equipment body by a naked hand Some other abnormality
41. nt which within one 1 year after delivery or fifteen 15 months after shipping whichever comes earlier is proved to the satisfaction of Kubota to have been defective at the time of delivery provided that it does not fall under the exceptions and conditions specified in this warranty Such exception and conditions include but are not limited to failure due to natural wear and tear accident negligence alteration repair or operation in a manner not prescribed in the Instruction Manual supplied with the instrument The foregoing expresses Kubota s sole warranty with respect to the instrument THIS WARRANTY IS MADE IN LIEU OF ANY AND ALL OTHER WARRANTIES AND ALL IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED AND EXCLUDED Kubota and its authorized dealers will not be liable for any consequential damages loss or expense arising from the improper use of the instrument Kubota will not honor any other warranty which may be given by its representative or dealer or otherwise which is different from the warranty given hereunder This warranty is not assignable and is operative only in favor of the original customer to whom this warranty is originally delivered Use of Model PlateSpin Model PlateSpin can be used for preprocessing the in vitro analysis which breaks down blood or urine samples containing plasmas or cells Accordingly it is not designed to connect directly to a patients body Don t us
42. ot adequately deal with such matters ourselves we may return the products to the customer without taking further action Contaminant elimination fee If we are forced to perform the work of eliminating contaminants we may request that you pay a fee Please note this stipulation beforehand Please make a copy of this page and fill out the relevant details Contaminant Elimination Certificate Date Name Company Institute Department Tel Fax Address We hereby certify that contaminant elimination for this equipment has been performed as follows Type of unit Serial No Type of unit Serial No Accessories Date when contamination elimination was carried out Date Contaminants Method used Dealer notification Signature 4 10 Section 5 Troubleshooting Ex TIME display TIME E M O sec Control panel Caution l Checkpoint Action taken Setting speed alarm An error occurs immediately after the START key is pressed when Pressi he SPEED TIME k the speed setting is more than ida MN e makes the centrifuge auto correct 2 500 thi h n when SUE QU MUCHO UDINE the speed to 2 500 rpm centrifugal force after changing rotation radius is changed to speed section 5 Troubleshooting 1 00 Alarm Indicators OU U When trouble occurs during operation the TIME display flashes to show the alarm The alarm indicators are mentioned in the table
43. pection fee required For the following inspection items please call your local dealer and set up an appointment for a periodical inspection Motor ope ed control Rotor and bucket Imbalance lid Electric current Chamber Insulation opeed meter Install the centrifuge Timer Section 4 Service 4 4 Cleaning and Sterilization NWARNING Consult with your nearest dealer prior to attempting any cleaning procedure for the rotor buckets or tube rack that is not specifically recommended in this manual Certain cleaning methods or cleaning agents may cause corrosion and then breakage leading to serious damage or accidents involving physical injury 1 Cleaning the chamber interior The chamber is made of stainless steel S8US304 but may rust if sample spillage containing salt NaCl or chlorinated chemicals Cl is allowed to remain After using the centrifuge follow the method below 1 when wiping the inside of the chamber and cleaning the surfaces 1 Cleaning and rust prevention Cleaning the chamber interior according to the following procedure will help prevent rust 1 Wipe off any dirt or residue using a cloth moistened with a small quantity of a neutral detergent 2 Wipe away any detergent residue using a cloth dampened with water and then dry the inside of the chamber 3 To prevent rust we recommend the use of common anti rust agents such as CRC 556 only if such will not affect the samples being centri
44. peration Press the STOP key on the control panel o STOP There is the sound and the rotor will come to a halt In case that the operation is stopped before the end of the period set by the timer and is resumed the operation period after resumption is the remained period of the operation If the knob is pressed before resumption of operation the operation period returns to the originally set period Section 3 Operation When the rotor has stopped the STOP lamp flashes and an sound that informs the end of the operation is issued The Sound that informs the end of the operation can be selected from the 5 kinds Sound none gt Refer to page 3 15 The Alarm Indicators to show the end of the operation can be selected from the 5 kinds P Refer to page 3 18 EN napi NN Operation 10 Press the OP EN button to open the lid S TOP Jamp remains lit Operation 11 Remove the sample gt Refer to page 3 17 Operation 12 Ifthe centrifuge is to be used again return to Operation 4 Operation 13 After finishing use of the centrifuge turn OFF the POWER switch to turn off the power supply NOTE The setting parameters are memorized when the power is tumed off and they will be called up and displayed when the power will be turned on next time section 3 Operation 3 3 Opening and Closing the lid L1 Turning on the power and opening the lid 1 Turn on the the POWER sw
45. r for inspection Over spee d alam Flashes to stop the rotation when the maximum speed of the corresponding rotor is exceeded Sp eed alarm This code when the equipment appears did not operation within 5 seconds after the START key was pressed by as electric circuit start its such causes failures Contact your local dealer for inspection when this alarm continues to flash After the rotor stops spinning turn off the power supply once and turn on the power after about 30 seconds If the alarm recurs contact your nearest dealer for inspection Cont d on next page section 5 Troubleshooting Alum Checkpoint Action taken Time alarm for rotor inspection Because the inspection time has arrived the alarm is displayed 1 5 represents the level of the number of times of rotor use As for the details please refer to the following The inspection time for the rotor has arrived Contact your nearest dealer for inspection of the rotor and bucket Alarm Indicators and Number of times of rotor use at the time of rotor inspection Each Alarm Indicator is displayed at the time when the number of times of rotor use reaches the figures shown below Number of times of rotor use at Indicators the time of the alarm indicators 25 000 25 050 C2 40 000 40 050 C3 50 000 59 999 C4 60 000 69 999 C5 From 70 000 0 00 Troubleshooting Section 5 Troubleshooting Is the power plu
46. rmation afte please contact your local dealer Lifetime of rotors A WARNING Use of rotors beyond the lifetime may lead to breakage of the rotor If the rotor is used continuously even after the lifetime of rotors has expired should the rotor get damaged the main unit of the centrifuge suddenly may start to rotate this could result in an accident causing injury or death Lifetime of rotors is 7 years after the delivery When 7 years have passed after the delivery discontinue operation of the centrifuge to replace the rotor with a new one Eardier replacement however is required if any corrosion lowered strength flaw or deform due to incorrect operation is detected on the rotor In such case contact your local dealer and be sure to have the rotor checked before reusing it Number of times allowed for autoclaving of rotor A WARNING Stop the use of the rotor immediately when it is used beyond the number of times allowed for autoclaving Otherwise the rotor may deteriorate by the heat generated by autoclaving resulting in deformation or destruction Should the rotor get damaged the main unit of the centrifuge suddenly may start to rotate this could result in an accident causing injury or death The number of times allowed for autoclaving of each rotor should be deemed as follows When the following conditions have been met discontinue operation of the centrifuge to replace the rotor with a new par
47. rpm O0 X10 g ILI U When continuing on to time setting press the FUNCTION key The SPEED display stops flashing and the TIME display starts to flash Timer setting status Q SPEED h TIME af LL x100rpm Y Winn FUNCTION 3 NV jum o X10 a p sec e RF o NOTE 6 The centrifuge can be started with the speed on the SPEED display even if the TIME display of value is flashing When you want to change the setting during the operation press the FUNCTION key 2 times on the control panel after changing the setting and the flashing of the display is stopped The operation becomes shifted to the setting that have been changed The setting order of the speed and the timer can be changed gt Refer to page 3 16 0 0 Section 3 Operation MMO Setting the speed by the centrifugal force x g OLU U Press the FUNCTION key The SPEED display flashes and indicates the current setting KSPEELDy T Y X100rpm FUNCTION a I5 k i i X10 g J o d OU LI Check if thel X 10 all lamp tumed on When thed X 10 dU lamp is on OLLUM Proceed to the Procedure L1 UU SPEED ai X100rom 16x 10 g 7 2 When thd X 10 gL lamp is of UOL Press the SPEED TIME knob SPEED and th X 10 glllamp turns on 45 x 100mm Proceed to the Procedure J UU 45 X10 g OU U Tum the SPEED TIME knob to set to required centrifugal force reading Indications on the SPEED display are at 10 X q intervals
48. s are present on the centrifuge and rotor reasonable care is required for their handling Otherwise failure may occur resulting in property damages or fatal physical injuries In order to prevent them from happening be sure to follow the instruction given below NWARNING r Maximum load 9 Do not exceed the maximum load of rotor and bucket The rotor or bucket used beyond the allowable load level can be damaged thereby causing an ac cident lt 23 Maximum speed o Keep the rotor and bucket speed below the maximum speed Excessive speed may cause dam age to the rotor bucket and the centrifuge The maximum speed depends on the rotor and bucket strength d Modification and unspecified parts Do not modify nor use unspeci fied parts Unauthorized retrofit of the centri fuge rotor or bucket or use of un authorized parts for them can re sultin accidents 4 Hazardous material Do not put any hazardous materi al explosive chemically active organic or radiation containing material or material contaminated by pathogenic microorga nisms as a sample of the centrifuge and do not place it closer than 30 cm to avoid a secondary accident should the centrifuge accidentally rotate and contact the material amp While the centrifuge is in operation o Et Lid gt While the centrifuge isin operation do not stand closer than 30 cm to avoid asecondary accident Do not open the l
49. t Earlier replacement however is required if any corrosion lowered strength flaw or deform due to wrong operation is detected on the rotor In such case contact your local dealer and be sure to have the rotor checked before reusing it OOU Number of times allowed for autoclaving and temperature of rotor Number of times Temperature of autoclave allowed for autoclaving OOO Recording autoclave After each autoclave process be sure to record the following I toU L to control how many times the autoclave is executed Ulp Date 100 Temperature of autoclave HEIL E Time of autoclave gt You can take advantage of using the Autoclave record table attached to this manual Section 1 Component Name and Explanation 1 1 Appearance Sool eS e x D Hook Lid ra Tachometer port P Refer to Page 4 7 Chamber J al amp OPEN button The lid opens when it is pushed during the operation stop with the power on P Refer to Page 3 4 Front cover P Refer to Page 3 5 Lid extreme release cap P Refer to Page 3 4 Control panel P Refer to Page 1 2 DOWER switch oection Component Name and Explanation 1 2 Control Panel SPEED TIME knob TIME display Setting the speed RCF and time p Refer to Page 3 6 SPEED display START key Speed RCF When this key is pressed the operation will be started p Refer to Page 3 2 ACC DE
50. te when samples are left inside the centrifuge after completion of the operation The reminder alarm goes off when opening the lid or performing some opera tions Factory default ONU LI IL Pressing the SPEED TIME knob while holding down the FUNCTION key The used number of times of the centn fuge is displayed OOU Pressing the SPEED TIME knob several times and let indicate DO on the TIME display The SPEED display indicates the current setting of the reminder ON or OFF see the figure below P Refer to Page 3 12 Ex L Reminder alarm ON SPEED TIME 3 X 100rpm FR O min C 3 o X10 a E O sec Y o 9 OUL Tum the SPEED TIME knob to change the number Ex Reminder alarm OFF SPEED TIME KN ME x 100mm 7 O min C o x10g 5 O sec OUL Press the SPEED TIME knob while holding down the FUNCTION key The display returns to the previous Speed and Time indication after the setting reminder on or off 1s saved Section 3 Operation OUL Setting the Indicators for the end of the operation Completion of the operation is indicated not only with an alarm but also with the SPEED and TIME displays The Alarm Indicators can be selected from the 5 kinds Factory default 1 see the figure below U L Pressing the SPEED TIME knob while holding down the FUNCTION key The used number of times of the centrifuge is displayed OUL Pressing the SPEED TIME knob sev
51. therwise the drive shaft may become bent or the rotor and the bucket may come off resulting in an accident or damage to the centrifuge Ensure that rotor and bucket are removed from the centrifuge and that the power cord is disconnected from the wall outlet e Moving the position of the centrifuge while the power is tumed on may cause electrification accident or functional failure of the centrifuge Cont d on next paae section 2 Installation and Power Supply CAUTION E When moving the centrifuge lift up the bottom of the centrifuge body by two or more persons using equal forces to move the centrifuge to the desired place ll Do not drop the centrifuge otherwise damage or injury may occur 2 4 Power Requirement A WARNING Prepare a power supply that meets the following conditions 1 The power supply voltage must be the same as that indicated on the nameplate of the centrifuge and the voltage variation must be within the range given in Table 2 1 2 The current capacity must be more than given in Table 2 1 3 In connecting the cord install a knife switch or circuit breaker of given value in Table 2 1 When the centrifuge is connected to an outlet by means of a plug use a 3 pin plug with a ground terminal 4 Use single phase power 5 The outlet must have a ground terminal and its ground resistance must be less than 100 ohm Rated Voltage 110 115 V 220 230 V Acceptable Voltage Range 99 V to 126 V
52. trifuge inspection before resuming operation of the equipment If the centrifuge is used after discovering any abnomnality an accident could occur possibly leading to serious damage or accidents involving physical injury Checkpoint Action taken Check whether the rotor locking nut is properly tight To inspect mount buckets on the swinging bucket rotor when it is stopped and lift up the buckets manually Check carefully for any cracks evidence of corrosion rust or deformation on the rotor and buckets Check that no foreign material or water is present in the chamber Check that the lid hook is locked Check that the screws fixing the lid hinges are not loose Check that the grounding wire is correctly connected Check that all knobs displays lamps and switches operate correctly Check that the centrifuge is placed horizontally Retighten if either of these 1s loose For details refer to Mounting the Rotor in the rotor instruction manual gt Refer to Section 6 Ifthe buckets do not move smoothly and freely clean the bucket grooves and trunnion pins of the rotor yoke using alcohol Contact your local dealer and make an appointment for an authorized inspection if these parts fail to operate smoothly even after lubricant has been applied P Refer to Page 4 5 Do not use the rotor or buckets if any cracks corrosion rust or deformations are found Contact your local dealer for an 1nspection Remo
53. ve any foreign material or water before operating the centrifuge Ifthe lid lock does not work discontinue operation of the centrifuge Contact your nearest dealer for a centrifuge inspection If the screws fixing the lid hinge are loose or have been removed the lid may come off when the buckets are thrown off and the broken pieces may cause injury to the persons in the vicinity of the centrifuge If the lid hinge is not fixed firmly stop centrifuge operation Stop operation promptly and contact your local dealer for inspection gt Refer to Paae 2 3 If any do not operate correctly contact your local dealer for an inspection gt Refer to Page 1 1 and 1 2 gt Refer to paae 2 1 Section 4 Service 0 gg Monthly Inspections uuu Inspect the rotor ZA NWARNING Perform a monthly careful inspection of the rotor appearance Check for any deformation or damage including the interior and bottom of the holes If any abnormality whatsoever is found there may be significant damage or comosion of the rotor that could lead to serious damage or accidents involving physical injury Stop operation at once put DO NOT USE labels on the rotor and centrifuge lid and contact your local dealer 1L Clean the rotor and buckets Remove the rotor from the shaft and clean it b Refer to Page 4 3 100 Clean the chamber interior gt Refer to Page 4 3 o O0 Annual Inspection Annual Ins
54. ve to do Details are shown near the mark using illustration or sentences Indicates caution warning and danger Details are shown near the mark using illustration or sentences This label indicates the risk of electric shock Touching this attached part will cause an electric shock This label indicates a hot section Touching this attached part will cause an burn Indicates that the power is on It is Indicated on the power switch and the circuit breaker Indicates that the power is off It is indicated on the power switch and the circuit breaker Table of Contents page Safety Instructions General Notes eee nenennnwV I Usable Rotor ene nennnBn wwe IV Lifetime of rotors n ne eee IV Number of times allowed for autoclaving of rotor V Section 1 Component Name and Explanation 1 1 i Appearance a 1 1 1 2 Control Panel a ooooooooooooo 1 2 Section 2 Installation and Power Supply 2 1 Unpacking 4 uos e 1 0 AVETE Ed 0 1 2 2 Place of Installation 5e5e 2 2 3 Movement of centrifuge Zk 2 4 Power Requirement 2 2 2 5 Grounding P E E E De Section 3 Operation 3 1 Cautions of Operation 3 1 3 2 Operation a a 3 1 3 3 Opening and Closing the lid 3 4 1 Turning on the power and opening the lid a cooooooooooooooooooooo 3 4 2 Opening the lid during power failure 3 4 3 Closing
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