CURRENT STIMULATOR - USER MANUAL
Contents
1. 14 3 2 Channel centered eee Lie odios Hoe the tiui en e teu ead ae aie do eee deve ieee 14 3 3 LN mU M m 17 3 4 CommunicaUom subfnelusqgs uu oie her aris acheter stevie ier tar ene Eo uc Sete eta 17 3 5 Keyboard Chapter 4 Serial protocol for 5200 18 41 5 EUN REA RE BENE RR BARRES eR T 18 1 2 Serial AAAKESSOS a aa t cae PG date Sedo ne we ieee Pee yep 18 2 3 Address chistes e iacet Coc Sex temer desi ias steve tec cras ier 19 Appendix 1 5 23 4 CURRENT STIMULATOR MODEL CS200 USER MANUAL SAFETY The Current Generator has been designed for use only in teaching and research applications It is not intended for clinical or critical life care use and should never be2. 3600 sec APPENDIX 1 23 5 Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China Tel 86 0 21 5425 1330 Fax 86 0 21 5877 0063 www dmt asia com sales dmt asia com support dmt asia com DMT USA Inc 525 Avis Drive Suite 10 Ann Arbor MI 48108 USA Tel 1 770 612 8014 Fax 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
3. Particular requirements In vitro diag nostic IVD medical equipment With reference to regulations in the following directives 2006 95 EC 89 336 EEC CERTIFICATE OF CONFORMITY 7 ABOUT THIS MANUAL This manual contains a complete list of procedures describing how to install and get started using the Current stimulator unit model CS200 Chapter 1 provides an introduction to the construction and basic features of the Current stimulator unit Chapter 2 describes step by step how to install a Current stimulator unit Chapter 3 is a complete manual to the Current Stimulator Unit The chapter describes in detail the construction of the menu system and how to use all the features of the Current Stimulator Unit Chapter 4 contains procedures describing how to set up a serial protocol and requesting data Appendix contain system specifications 8 CURRENT STIMULATOR MODEL 5200 USER MANUAL UNPACKING THE CURRENT STIMULATOR SYSTEM Please take a few minutes to carefully inspect your new Current stimulator unit for damage which may have occurred during handling and shipping If you suspect any kind of damage please contact us immediately and we will take care of the problems as quickly as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Current stimulator unit In case of transport a
4. used for these purposes nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap e Do not open the unit the internal electronics pose a risk of electric shock Donotuse this apparatus near water To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus e Do not block any ventilation openings Install in accordance with the manufacturer s instructions Donotinstall near any heat sources such as radiators heat registers stoves or other apparatus that produce heat e Only use attachments and accessories specified by the manufacturer Unplug this apparatus during lightning storms or when unused for long periods of time This apparatus must be grounded e Useathree wire grounding type cord similar to the one supplied with the product Do not defeat the safety purpose of the polarized or grounding type plug A polarized plug has two flat blades one being wider than the other A grounding type plug has two blades and a third round grounding pin The wide blade or the third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet e advised that different operating voltages require the use of different types of line cord and attachment plugs Check the voltage in your area and use the c
5. A 50 mA Output Train mode CONT Train sec Train 180 sec Start Freq 0 5 Hz Stop 64 Hz Repeat dly 120 sec 14 CURRENT STIMULATOR MODEL CS200 USER MANUAL Frequency defines the number of pulses per second Hz Pulse Pulse duration defines the length of the pulse in msec Current defines the amplitude of the pulse in milliampere Current 7 Pulse amplitude Unit mA lt gt Pulse duration Unit ms Figure 3 2 Single current pulse e Output polarity is either MONO polar meaning that current flows one direction or BIPOLAR meaning that current alter nates in one and then the other direction Monopolar Bipolar Figure 3 3 Train mode Train mode A pulse train consists of pulses with a frequency pulse duration current amplitude and polarity as defined in the menu items described above Three train modes can be entered which are described below e OFF The pulse stimulator runs continuously without interruption SINGLE The pulse stimulator runs a single train of pulses defined by the menu items above Train e CONT The pulse stimulator runs pulse trains with the length and delay that are indicated in Train dur and Train dly Freq Step The pulse stimulator runs a frequency ramp of pulse trains starting with a frequency defined in Start freq and doubling th
6. DMT MYO TECHNOLOG CURRENT STIMULATOR COMBINED PULSE amp TRAIN GENERATOR MODEL CS200 User Manual Version 1 8 2 CURRENT STIMULATOR MODEL CS200 USER MANUAL CURRENT STIMULATOR MODEL CS200 TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 25 are trademarks of ADInstruments Pty Ltd Pentium is a registered trademark of the Intel Corporation Windows Windows 95 Windows 98 Windows ME Windows NT Windows 2000 Windows XP and Vista are registered trademarks of Microsoft Corporation All other trademarks are the properties of their respective owners DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the DMT Current stimulator Model 5200 Document number CS200 002A All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as required and without any notice Copyright Danish Myo Technolo
7. ammed protocols have been determined they can be stored in one of five internal program memories to be re loaded at any time The CS200 can easily be connected to a computer using the standard serial port either alone using RS232 serial protocol or in combination with up to 4 additional stimulators and maximal 4 myograph systems using the RS485 serial protocol 1 3 Features The following features are common on the CS200 Current Stimulator Pulse stimulator Pulse width 0 03 500ms Current O 100mA Compliance 50 Volts e Rise fall time 1 5us Output mono or bi polar Train stimulator Frequency O 1 256Hz Train duration 0 1 3600s Train delay 0 1 36005 Frequency ramp Internal memory can store up to five user defined current waveform protocols External control through serial communication RS232 RS485 10 CURRENT STIMULATOR MODEL CS200 USER MANUAL CHAPTER 2 INSTALLATION PROCEDURE 2 1 Current Stimulator front panel i C M down arrows Will move the cursor in the display to the next or previous menu item LCD display Mic HE ENSE Channel and CS200 setup menu displayed here Numerical keypad Used to enter numerical values for various menu items in the channel or setup menus Power indicator 9 A yellow LED indicates power switched ON Channel selectors E Change the active channel menu shown in th
8. d 5 and press Enter Get prog By finding the appropriate program number 1 to 5 and pressing Enter one of the stimulation protocols that are stored in the memory can be retrieved e Syncron When this menu item is enabled by setting its value to YES pressing Start will result in simultaneous starting of all channels When Syncron is set to NO pressing Start will only start the channel that is visible in the LCD display Option contains service and communication submenus 3 4 Communication submenu To reach the communication submenu press 7 Enter under options in the setup menu COMM MENU Serial mode gt RS485 Channelno 1 4 Serial mode Here the RS232 or RS485 serial protocol is set Using RS232 only 1 serial device e g one current stimula tor can be connected to one PC serial port see 2 3 2 PC Controlled single device on page 12 PC controlled single device Choosing RS485 enables connecting multiple serial devices to a single PC serial port To chose between RS232 and RS485 press Enter Channel no This line is only visible if RS485 is chosen in the previous menu Serial mode Channel numbers for the current stimulator are defined in this menu Choose channel numbers for 5200 1 4 5 8 9 12 13 16 by pressing En ter Thus 4 CS200 can be connected in series to one PC serial port together with four 610 620 700 0 720 0 and 800MS 820MS systems The channel number
9. e Pod d Channel output LCD display by pressing these buttons i EE t o5 Po Four shielded standard BNC channel output t qo P f signal connectors o1 Cof i Redes e Esc Channel Guant Output indicators o E Go f j Undo all changes to the menu items by pressing the A red LED indicates active pulse train generation iod t od escape Keys Setup e eel Enter the CS200 setup menu by pressing this button Single 3 tor Starts output of a single current pulse of the channel 20107 shown in the LCD display When SYNCRON in the setup Start menu is set YES all channels will start simultaneously Start current stimulation using the stimulation proto i tune a sinele curent pulse outout col of the channel currently visible in the LCD display i giving 8 pus When SYNCRON in the setup menu is set to YES E all channels will start simultaneously Enter E VENI O A E Any changes of the values of menu items in the LCD Stop current stimulation of one or all channels display will be permanent after pressing the Enter key until a new change is made Figure 2 1 Current Stimulator front panel CHAPTER 2 11 2 2 Current Stimulator rear panel 5E Ground Ground can be connected to the common ground that con nects all grounded equipment in
10. e frequency until the frequency defined in Stop freq is reached see figure 3 5 on next page CHAPTER3 15 Train Mode SINGLE CONT Figure 3 4 Train dur Train duration is entered in seconds It indicates the length of the pulse train see black bars below CONT mode in fig 3 4 Train dly Train delay entered in seconds This determines the interval between pulse trains see white bars below CONT Train mode in figure 3 4 above Start freq This menu item is only of relevance if Freq step is chosen in Train mode otherwise this parameter is ig nored Start frequency defines the frequency at which the frequency ramp starts Stop freq Final step frequency of the frequency ramp Repeat dly Delay between frequency ramps in seconds If this parameter is set to O the frequency ramp runs only once Train Mode Freq step Start Stop freq LITT AUO Figure 3 5 Pulse trains with increasing frequencies The length of the white bar below equals Train dur the black train delay 16 CURRENT STIMULATOR MODEL CS200 USER MANUAL 3 3 Setup menu SETUP Saveprog 1 Get prog ee Syncron Chan 1 Option 8 amp 99 Save prog Up to 5 stimulation protocols including all channel parameter information can be stored in an internal memory Store the present channel programs by choosing a number between 1 an
11. ency of 200 Hz and pulse duration of 10 will also results in a ERROR message in the entry line Thus there always has to at least 1 ms between pulse duration and the reciprocal frequency i e 1 frequency e The first line of the LCD display will show blinking asterisks indicating that a stimulation protocol has been started and is running The display indicates RESISTANCE TOO HIGH if the electrical resistance at the electrodes is too high for the current that has been set in the stimulation protocol Typically this occurs if electrodes are not immersed in physiological salt solution i e if the Current Stimulator is started with the electrodes in air 3 2 Channel menu Every available channel has its own channel menu that can be accessed by pressing the appropriate channel selector on the front panel see page 11 The channel number will be displayed in the first line of the LCD display Every channel pulse protocol can be changed individually by pressing the respective channel selector and entering the appropriate values for the pulse and train parameters for that particular channel Alternatively all channel pulse and train parameters can be changed simultaneously if SYNCRON is set to YES in the Setup menu In this case entering values for a parameter in the channel menu of CHANNEL 1 will also change the same parameter in all other channels available CHANNEL1 Frequency 100 Hz Pulsedur 0 1 ms Current
12. gy A S IAN ISH M14 OT CHNOLULOSY TRADEMARKS 3 CONTENTS Certificate of C nforMity TTD EI EE G 7 5 8 Unpacking the Current Stimulator system enr eie Introd eT PERPE E Ms 10 4 1 Docuiment SCOPE sais ii iieri itr EE Ro rEEe Fete CHELSEA Ye VATER FRE RR 10 1 2 nd foro o o OA ARIES ORDRE PC 10 2 3 LIU CR CE E 10 Chapter 2 Installation procedure 11 2 1 C rrent Stimulator front palel caecus 11 2 2 Cunrent stimulator rear nisin 12 2 3 nstallati O Meara LL 12 3 Using the Current Stimulator 22 1 c orccueptnu cecinere cauce uo ark ac sc ca us acean arr ao ve 14 SU CDi GiS Play e
13. nd the original packing material is unavailable please contact DMT Sales Department for advice and packing instruc tions After unpacking your new current stimulator unit please use the following list to check that the system is complete e 1 Current stimulator unit e 1 power cord e 1 User manual The shape of the AC plug varies by country be sure that the plug has the right shape for your location UNPACKING THE SYSTEM 9 CHAPTER 1 INTRODUCTION 1 1 Document scope This manual provides the information required to install and understand the principles of operating the 5200 combined pulse and train stimulator 1 2 Product overview The CS200 Current Stimulator combines simple and intuitive user operation with sophisticated features that are required in elec tro physiology The CS200 is a modular and highly versatile 4 channel current stimulator designed for use in combination with DMT s Myograph systems It is optimized for electrical field stimulation of vascular smooth muscle isolated skeletal muscle or cardiac muscle Furthermore for controlled neurotransmitter release from perivascular nerve endings or skeletal muscle motor nerve end plates The stimulator provides a variety of stimulation modes and protocols such as single continuous and frequency regulated steps All parameters are easily entered from the front panel and can be applied on an individual channel basis or to all channels simul taneously Once the progr
14. nt off and on the user is encouraged to correct the interference by one or more of the following measures e or relocate the receiving antenna e Increase the separation between the equipment and receiver Connectthe equipment into an outlet on a circuit different to that which the receiver is connected to Consult the dealer or an experienced radio TV technician for help APPROVALS Complies with the EMC standards EMC 89 336 EEC EN 50 081 1 and EN 50 082 1 FCC part 15 Class B CISPR 22 Class B Certified with the safety standards 60 065 IEC 60065 Complies with the safety standards 116500 CSA E65 6 CURRENT STIMULATOR MODEL CS200 USER MANUAL CERTIFICATE OF CONFORMITY DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Current Stimulator Model CS200 is covered by this certificate and marked with CE label conforms with the following standards EN 61010 1 2001 EN61010 1 Corr 1 2003 Safety requirements for electrical equipment for measurement con EN 61010 1 Corr 1 2003 trol and laboratory use Part 1 General requirements EN 61010 2 101 2003 Safety requirements for electrical equipment for measurement con trol and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment EN 61326 2 6 2005 Electrical equipment for measurement control and laboratory use EMC requirements Part 2 6
15. orrect type See the table below Line plug according to standard 110 125V UL817 and CSA C22 2 No 42 220 230 V CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 240 V BS 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets Protect the power cord from being walked on or pinched particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped SAFETY 5 EMC EMI This equipment has been tested and found to comply with the limits for a Class B Digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by monitoring the interference while turning the equipme
16. p Internal address see Table 2 X Program number cr Carriage return Example Command 80841 Response gt 80 All channel parameters will be stored program 1 NOTE THAT BECAUSE STORING DATA TAKES ABOUT THREE SECONDS THE RESPONSE WILL BE DELAYED ACCORDINGLY CHAPTER 4 21 4 3 5 Error status Any internal error in the stimulator can be retrieved with the following commands Command Response For which aa pp Xy cr Example Command Response aappx cr aa cr Set data Channel address see Table 1 Internal address see Table 2 Error parameters 0 No timer errors 1 Internal timer communication error 2 Pulse duration too long or frequency to high o x 3 Pulse duration too long in FREQUENCY STEP MODE oy O Noresistance error 1 Resistance in the chamber is too high Carriage return 8087 cr gt 8001 cr The electrical resistance between the electrodes is too high at channel 1 22 CURRENT STIMULATOR MODEL CS200 USER MANUAL APPENDIX 1 SYSTEM SPECIFICATIONS Pulse generator Frequency Pulse duration Current Compliance Rise fall time Output Voltage 0 1 256 Hz 0 03 500 msec 0 100 mA 50 Volts 1 5 msec Mono Bipolar 100 to 240 VAC auto 50 60 HZ Train generator Mode 1 Mode 2 Mode 3 Mode 4 Train duration Train delay Off Single Continuous Frequency step 0 1 3600 sec 0 1
17. pin socket The CS200 operation can be controlled for example through the serial communication program HyperTerminal Windows using the commands explained in detail at next page 12 CURRENT STIMULATOR MODEL CS200 USER MANUAL To any available PC serial port CS200 rear view CS200 serial output Figure 2 3 PC control through RS232 serial connection CHAPTER2 13 CHAPTER 3 USING THE CURRENT STIMULATOR 3 1 LCD display TheLCD display is the user interface of the CS200 for manual control of the Current Stimulator The first line of the display always indicates the menu item currently selected Menu items can be divided into channel menus and setup menus The second line is always the INPUT line The input line is indicated by gt instead of The numerical key pad is used to enter new data at the input line Following data entry Enter is pressed to store the new data entry at the input line Pressing Esc can erase an erroneous data entry All non numerical menu items e g Output Mono or Bipolar can be changed by pressing Enter until the desired change e Scroll through the menu displayed in the LCD display by pressing the up or down arrows respectively e The LCD display reads ERROR on the entry line if entered data is outside the range indicated by the specifications of the CS200 Current Stimulator Frequency and pulse duration that are not compatible with each other e g frequ
18. s that are entered in this menu will be visible at the first line of the channel menu 3 5 Keyboard Start The Current Stimulator starts electrical stimulation by pressing the start key on the keypad Depending on whether Syncron is set to Yes or No all channels will start simultaneously or one channel the channel currently active in the LCD display will start STOP Ends current output from the active channel in the display or ends current output from all channels if Syncron was set to Yes Single pressingthis key will start only a single pulse of the active channel in the display with the pulse length defined in the channel menu Again if Syncron in the setup menu was set to Yes a single pulse will start on all channels simultaneously 17 CHAPTER 4 SERIAL PROTOCOL FOR CS200 The CS200 Current Stimulator can be controlled remotely through a PC serial connection Depending on whether the RS232 or RS485 serial protocol are chosen either a single or multiple current stimulators connected in series can be controlled when connected to a single PC serial port see Figure 2 3 PC control through RS232 serial connection on page 13 CS200 operation can be controlled for example through the serial communication program HyperTerminal Windows using the commands explained below 4 1 PC serial port The PC Serial port parameters should be set to the following values Bits per second 9600 Da
19. ta bits 8 Parity none Stop bits 0 Flow control none 4 2 Serial addresses The current stimulators receives the address range 80 90 with a possibility to connect four CS200 to the same PC serial port CS200 Channel 1 4 3180 4283 single stimulator addresses Channel 5 8 284 487 Channel 9 12 88 88B Channel 13 16 8C 8F Table 1 Channel addresses Version No 00 Frequency 01 Pulse dur 02 Current 03 Output 04 Train mode 05 Train dur 06 Train dly 07 Start freq 08 Stop freq 09 Repeat dly 10 Start 80 Stop 81 Single 82 Syncron 83 These three parameters Save program 84 only in combination Get program 85 with 80 Error status 87 Table 2 internal addresses non hexadecimal 18 CURRENT STIMULATOR MODEL CS200 USER MANUAL 4 3 Address format 4 3 1 Requesting data Command aapp cr Response gt For which Request data aa Channel address see Table 1 pp Internal address see Table 2 550 Data returned from CS200 length depending data type cr Carriage return Example Command 28003 cr Response gt 80 03 0020 cr The value for Current on stimulator 1 channel 1 is requested Response is 20 mA Command 8005 cr Response gt 80 The status of Train mode is requested The reply can be C for CONTINUE O for OFF S for SINGLE F for FREQUENCY STEP CHAPTER 4 19 4 3 2 Set
20. the experimental set up Power switch and plug AC e Serial output RS485 Y DB9 serial output allowing interaction with the serial port of a PC The RS485 serial protocol al lows several serial instruments to be connected in series The use of RS485 serial output requires RS485 converter that is connected between the PC serial port and the serial instrument Serial output RS232 DB9 serial output allowing interaction with the serial port of a PC The RS232 serial protocol allows interaction of a PC with one serial device Serial commands and references are ex plained in detail Figure 2 2 Current Stimulator rear panel 2 3 Installation 2 3 1 Stand alone manual control Usethe supplied power cord for connecting the CS200 Current Stimulator to the power supply Be sure that the power cord is compatible with your local power supply e Connect channel outputs to the electrodes of your experimental set up using a BNC connector Typically platinum electrodes fit into an organ bath 2 3 2 PC Controlled single device A PC can control the function of the CS200 Current Stimulator If only one serial device is connected to the PC serial port the RS232 serial output situated on the backside of the Current Stimulator should be used Connectthe R232 serial output to the PC serial port with a 9 pin DB9 serial cable Use a DB9 to DB25 converter if the PC serial output consists of a 25 pin instead of a 9
21. ting data Command Response gt aa cr For which Set data aa Channel address see Table 1 pp Internal address see Table 2 XXXX X New values for stimulator length depending on data type cr Carriage return Example Command 8003100 cr Response gt 80 Current channel 1 of stimulator 1 is set to 100 mA Data sent to the Current Stimulator will NOT be changed if they are outside the ranges of the respective stimulation parameters Command 8005C cr Response gt 80 This command will set the train mode on channel 1 to CONTINUE Command 8086C cr Response no response 20 CURRENT STIMULATOR MODEL CS200 USER MANUAL 4 3 3 Additional commands Start Channel 1 Command 8080 cr Response gt 80 Stop Channel d Command 8081 Response gt 80 Check status of channel 1 Command 8080 cr Response gt 80Y cr channel 1 is On Y Yes or gt 80N cr channel 1 is Off N No Synchronize channels Syncron Command 8083Y cr Response gt 80 Do NOT synchronize channels Command 8083N cr Response gt lt 80 4 3 4 Saving and loading stored programs Stimulation programs that are uploaded from a PC can be stored in or retrieved from internal memory using the following com mand Command aappx cr Response gt For which Channel address see Table 1 p
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