User Guide for PowerTome Ultramicrotomes Models X
Contents
1. Figure 7 Stereomicroscope Adjustments Stereomicroscope and Holder The stereomicroscope and holder have numerous adjustments making it easy for you to achieve and retain an optimal view of your specimens The stereomicroscope can be swung to the left and right It may also be tilted up and down This movement allows you to set the optimum viewing angle for approaching your knife to the specimen and to select the best angle for viewing the sections as they float out on the water during sectioning The eyepieces are adjusted by moving them together or apart to match the distance between your eyes When first looking through the stereomicroscope you may not recognize what you see if it is set at too high a magnification or is out of focus To set the lowest magnification rotate the Zoom Knobs downwards Now rotate the Focus Knobs up and down to focus To position the knife edge or specimen in the center of view you can rotate the Forward Travel Knob to move the stereomicroscope towards or away from you Coarse positioning of the stereomicroscope from left to right is best done by simply grasping it and pulling it right or left until it is approximately centered over the knife and specimen Precise fine centering is easily done by turning the small Lateral Adjustment Knob on the right underside of the stereomicroscope holder 16 PowerTome User Guide Section 3 PowerTome Basics RMC Products Sectioning Overhead Diffuse Lig2. The arc segment mount is stored in your accessory box To use the arc segment mount insert the mounting pin into the end of the cutting arm Normally you may want the arc segment mount oriented so that the Specimen Rotation Knob is on the right side and the Arc Adjustment Knob is on the left side with the arc positioned vertically To secure the arc segment mount to the cutting arm tighten the Locking Screw For special purposes you may want the arc oriented horizontally such as to cut your specimen block at a different angle You may orient the sample at angles up to 45 by tightly screwing another mounting pin PN 75155 into the back and inserting this pin into the cutting arm Your Specimen Holder will fit into the arc segment mount If the specimen holder has a 10 mm mounting pin it may be inserted directly into the arc segment mount If not an Adapter PN 75363 must be screwed tightly onto the thread To mount a specimen holder insert it into the arc segment mount and rotate the specimen rotation knob until you can see a set screw through the specimen locking screw hole Firmly tighten this with the wrench provided in your accessory box PowerTome User Guide 15 Stereomicroscope and Holder RMC Products Section 3 PowerTome Basics Now try out the specimen rotation knob and the arc adjusting knob to see how these adjustments allow you to rapidly adjust your specimen to the desired orientation for sectioning
3. 1 RMC Products Section 1 Introduction For additional information call 1 520 745 0001 in the continental United States In other countries contact your local RMC Products dealer Current dealers can be found at www rmcproducts com dealers html 2 PowerTome User Guide Section 2 Unpacking Installation amp Hookup RMC Products Unpacking Ultramicrotome Crate SECTION 2 UNPACKING INSTALLATION amp HOOKUP The unpacking and installing of the Power Tome Ultramicrotome is easy but requires two 2 people due to its weight Carefully Consider Location Ultramicrotomes because of their precision design are sensitive to both vibration and thermal changes The location and placement of the PowerTome plays a very important role in the instrument s performance An air isolation table is an ideal location However if no air table is available the second best option would be to place it on a sturdy work table with a rigid top Make sure the table can support the unit s weight If the PowerTome is placed on its own independent table make sure the table is not in contact with any walls surrounding tables or benches If considerations force it to be placed on a common laboratory bench that is connected to the floor or wall make sure there is no other equipment running during sectioning for example pumps or motors Avoid placing the microtome in areas of heavy traffic or where heavy rotating machinery is nearby suc
4. Arc Rotation Knob 2 Clearance Angle Adjustment Knob 11 Transilluminated Specimen Holder 3 Clamping Screw for Water Trough 12 Knife Holder Filler or Specimen Viewing Mirror 4 Water Trough Filler not included 13 Knife Clamping Knob 5 Diamond Knife not included 14 Knife Holder Clamping Knob 6 Arc Adjustment Knob 15 Back Light Cable 7 Specimen Locking Screw 16 Locking Knob Upper Stage 8 Arc Segment Mount 17 Lateral Knife Angle Adjustment Knob 9 Locking Screw Arc Segment Mount 18 Lower Stage PowerTome User Guide 11 Unpack Control Unit Make Connections RMC Products Section 3 PowerTome Basics Lower Stage Photo with Labels Figure 5 Lower Stage 1 Lower Stage 2 Fine Adjustment Knob 3 Coarse Adjustment Knob 4 Lateral Adjustment Knob 5 Locking Knob Upper Stage 12 PowerTome User Guide Section 3 Power Tome Basics RMC Products Upper Stage Power Tome Elements Handwheel Each rotation of the handwheel causes the cutting arm to go through a complete stroke up and down The up stroke corresponds to the retract half of the cutting stroke The cutting arm visibly retracts by a minimum of 80 pm during this part of the stroke Ifthe handwheel is left in any part of the up stroke it will beep two short beeps every 10 seconds to remind you that the arm is in a retracted position and you should not advance the knife to the specimen I
5. The PowerTome XL controller is equipped with an RS 232 port for communication with a personal computer Using this feature sectioning data can be viewed and stored The port receptacle is located directly below the interconnect cable receptacle on the back of the control unit Plug the male end with pins of the RS232 port cable into the receptacle located on the control unit Plug the female end with sockets into the appropriate receptacle on your personal computer 24 PowerTome User Guide RMC Products Appendix 1 Specifications APPENDIX 1 SPECIFICATIONS High precision micrometer knife stage Zero backlash stage drive Fine calibration 0 5 umlincrement Coarse calibration 5 umlincrement Gross advance 50 mm Fine advance 14 mm under micrometer control Clearance angle adjustment 2 to 15 Lateral left and right movement 30 mm Accepts glass knives up to 12 5 mm Compatible with all brands of diamond knives Full 360 rotation of knife with 45 graduated scale Dual frame vibration isolation Two piece instrument with separate control unit Energy dispersing polymer pads Isolation frame Floating cabinet Cast thermomechanical superstructure Adjustable lighting system with scan and tilt stereomicroscope Independently adjustable overhead diffuse lamp Intense cold backlighting with LED lighting LED transillumination from within the specimen holder Fast acting zoom from 10x to 64x 16x wide
6. field eye pieces Unique lateral scan of knife edge up to 30 mm with precision control knob Microscope pod swivels from left to right 90 Microscope tilts towards user 90 for optimal viewing PowerTome User Guide 25 Appendix 1 Specifications RMC Products Microprocessor control Cutting speed range 0 1 49 9 mm sec Variable return speed selection over entire cutting speed range Automatic advance Advance warning Section thickness range adjustable from 1 to 9999 nm Retraction 80 um Cutting zone 0 15 mm with resolution of 0 05 mm Cuffing window easily set with two buttons Four memory channels to store section thickness and cutting speed Power driven cutting stroke Built in service routines Advance totalizer and section counter XL only RS 232 Interface XL only Dimensional data Microtome 36cm x 43cm x 61 cm Controller 20cm x 12cm x 36cm Gross weight microtome crate 60kg Gross weight accessory box 17kg Net weight microtome 33kg Electrical Data Input 100 240 VAC 50 60 Hz Output 55 Watts maximum Power Supply UL listed CSA and CE norm certified 26 PowerTome User Guide RMC Products Appendix 2 Diagnostic Tests APPENDIX 2 DIAGNOSTIC TESTS Following is a listing of 10 self diagnostic tests that the PowerTome Ultramicrotomes can perform along with a brief explanation of each plus instructions explaining how to perform each routine Test Test Type Descripti
7. liability under no circumstances will exceed the contract price for the goods for which liability is claimed FOR TECHNICAL ASSISTANCE PLEASE CALL 1 520 745 0001 WARRANTY REGISTRATION Name Institution Department Address City State Zip Country Telephone Fax E mail Contact Person Model Serial Number Delivery Date Signature Date Please copy and Fax to 520 745 0004 or mail to Boeckeler Instruments Inc 4650 S Butterfield Drive Tucson AZ 85714 USA PowerTome User Guide iii PowerTome User Guide RMC Products Boeckeler Instruments Inc iv PowerTome User Guide 4650 S Butterfield Drive Tucson Arizona 85714 U S A O C GE r Tel 520 745 0001 Fax 520 745 0004 Email info boeckeler com DECLARATION OF CONFORMITY WE Boeckeler Instruments Inc 4650 S Butterfield Drive Tucson Arizona 85714 U S A Declare under our sole responsibility that the products PT X PT XL and PT PC Ultramicrotomes CR X Cryosectioning System GKM Glass Knife Maker To which this declaration relates is in conformity with the following standards or other normative documents EMC Directive 89 336 EEC amended by Directive93 68 EEC Low Voltage Directive 73 23 EEC Medical Device Directive 93 42 EC Class 1 Following the provisions of the EEC Directive 1999 5 EC Place and Date of issue As above 1 June 2002 Name Position and signature of author
8. select button and press the reset button to reset the section counter to Zero Both the advance totalizer and section counter are reset whenever the arm is reset 20 PowerTome User Guide Section 3 PowerTome Basics RMC Products Water Trough Filler optional accessory Other Components Water Trough Filler optional accessory Figure 9 Water Trough Filler The water trough filler allows for very precise control of the water level in the troughs of both glass and diamond knives This precise control is very helpful in keeping the knife edge wet while still keeping the water level as low as possible to avoid wetting the block face To use the water trough filler first remove the cap to the water reservoir and fill the reservoir with distilled water Replace the cap The water trough filler post plugs into the hole on the top left side of the upper knife stage and is secured by the clamping screw This post positions the stainless steel delivery tube over the trough The stainless steel delivery tube can be moved through the spring loaded positioner on the side of the post The delivery tube and post can be rotated in three dimensions to position the tube nozzle over the water trough Tighten the clamping screw on the left side of the upper stage and the positioning knob on the post to secure the delivery tube To deliver water to the trough rotate the pump knob clockwise To lower the water level in the trough rota
9. 0036 1 12 Accessory Kit 75593 1 PowerTome User Guide 33 Appendix 5 PowerTome X Shipping List RMC Products 34 PowerTome User Guide RMC Products Appendix 6 PowerTome XL Shipping List APPENDIX 6 POWERTOME XL SHIPPING LIST Item No Description Part No Qty 1 Power Tome XL Assy including 75501 1 2 Lower Stage Assy 75637 1 3 Upper Stage Assy 75635 1 4 PowerTome XL Controller 12V 75670 1 5 Stereomicroscope S6E 60460 1 6 Breath Shield Knife Stage 75205 1 7 Breath Shield Microscope Housing Assembly 75693 1 8 Dust Cover 75590 1 9 User Guide 051606 1 10 Power Cord 55AH044 1 11 Cable Interconnect 50036 1 12 Accessory Kit 75593 1 13 Dual Foot Switch Control 79960 1 PowerTome User Guide 35 Appendix 6 PowerTome XL Shipping List RMC Products 36 PowerTome User Guide RMC Products Appendix 7 PT PC Interface Module Hookup APPENDIX 7 PT PC INTERFACE MODULE Hookup This diagram illustrates the cables and connections required for the operation of the Power Tome PC The CR X unit is optional and its connections can be ignored if it is not included in your configuration The interface module also includes connections for the PowerTome foot switch Other connections for the CR X module include one for the pump and one for the dewar level Computer Null Modem Serial Cables UMT COM1 CRX COM2 PT PC I
10. AG einen ee E 14 Are Segment Mount een ungen EEEE enegueny sup canuduasenetwdes et 15 Stereomicroscope La ccc ct INN TERN EEE REN idea eE EEEE REER RARER ESA 16 Overhead Diffuse Lip htni eese tc een 17 Control Panel Elements naeh een 17 SOCHUONING ARRESTERE RER ERE O FE iecteevsdaseayesncdunnaned A TREUEN SERENE NEDE ESS SEERE RESEN 17 CUB RE RER E E E EIER venues Daas cardatcetiton E E T E AA E vanes 17 Foot pedal sristi aE EEEE EAR E E R 17 Ct Window een 17 Cutting Speed ee ee een 18 Section ThickhEsSi ee ernennen 18 Memory Storage nahe aE EEEE EEEE E AE AEN EE E 18 Lighting Ste ne ee 19 PowerTome User Guide vii PowerTome User Guide RMC Products Boeckeler Instruments Inc Oyethead Diffuse Light eine herein 19 LED Lighting Backlight Iransilumination nenne e 19 Sen ANNE ae naeh E EEE AE EN 19 Left Butto Deses aise E E TA AN T A AS 19 Bicht Dit onen een rset E ea S 19 Reset si einen energie kertseht oki 20 Advance Totalizer and Section Counter XL only een 20 SS Kreta 2 Lo ee REES En E TERRE REESE RES KERNE SETE SEN EDER ESS SES 20 Reset Button en hi nenne 20 Other Components an ee ee ee 21 Water Trough Filler optional accessory se rennen 21 Specimen Viewing Mirror optional accessory ann een nern 23 RS 232 Ports XL on Werne anne use nennen ev epee ae sadel 24 APPENDIX I SPECIFICATIONS nee 25 High precision micrometer knife stage ee 25 Du alframe vibration solle aaa 25 Adjustable lighting system wit
11. Arc Segment Mount 75150 Arc Segment Mount will also accept Leica Ultracut specimen holders 61823 3 16 Allen Wrench 75353 Trimming Post Fits onto trimming block For solid post specimen holders 56AC100 1 6A 250V SLO BLO Fuse 75413 7mm Flat Specimen Transilluminated Holder solid mounting post 50042 Flourescent Lamp 75155 Arc Segment Mounting Stud 75815 LED Lighting Cable Background 75816 LED Lighting Cable Transillumination PowerTome User Guide 29 Appendix 3 PowerTome X XL Accessory Kit RMC Products 30 PowerTome User Guide RMC Products Appendix 4 PowerTome X XL Accessories for reorder APPENDIX 4 POWER TOME X XL ACCESSORIES ror roren Part Description 75368 Universal Transilluminated Specimen Holder solid mounting post 75412 5mm Flat Specimen Transilluminated Holder solid mounting post 75413 7mm Flat Specimen Transilluminated Holder solid mounting post 70922 Wrench for Part Nos 75368 75412 and 75413 Specimen Holders 70594 5mm Round Collet type Holder threaded post requires P N 75363 Adapter 70595 6mm Round Collet type Holder threaded post requires P N 75363 Adapter 70596 8mm Round Collet type Holder threaded post requires P N 75363 Adapter 70597 Vise type Flat Specimen Holder threaded post requires P N 75363 Adapter 16801 Wrench for Vise type Holder 16826 8mm Collet type Specimen Holder red anodize
12. Inc will repair or replace and return free of charge any part which is returned to its factory within said period transportation prepaid by user and which is found upon inspection to have been defective in materials and workmanship This warranty does not include normal wear from use nor does it apply to any instrument or part which has been altered by anyone other thana Boeckeler Instruments Inc employee The warranty does not apply to any instrument which has been damaged through accident negligence failure to follow operating instructions the use of electric currents or circuits other than those specified on the plate affixed to the instrument use beyond the specified capacity of the instrument misuse or abuse Boeckeler Instruments Inc reserves the right to change alter modify or improve any of its instruments without any obligation whatsoever to make corresponding changes to any instrument previously sold or shipped The foregoing obligations are in lieu of all other obligations and liabilities including negligence and all warranties of merchantability or otherwise expressed or implied in fact or by law and state our entire and exclusive liability and buyer s exclusive remedy for any claim or damages in connection with the sale or furnishing of goods or parts their design suitability for use installation or operation Boeckeler Instruments Inc will in no event be liable for any special or consequential damages whatsoever and our
13. Tome User Guide 13 Lower Stage RMC Products Section 3 PowerTome Basics There are two Stage Rotation Knobs located on either side of the upper stage These permit precise rotational alignment of the knife to the specimen The small knob on the left side of the upper stage is the Clearance Angle Adjustment Knob To set this angle first remove the LED backlight cable from the knife holder then loosen the small Knife Clamping Knob located on the right side of the upper stage which secures the Knife Holder in place Next set the Clearance Angle Adjustment Knob to the desired angle For most glass knives 4 is a good angle to try first The recommended clearance angle for a diamond knife is usually written on its storage box It is typically 4 or 6 Next tighten the knife clamping knob Finally replace the LED backlight cable into the knife holder Lower Stage The lower stage incorporates a system of special roller bearings for maximum stability It can be shifted to the left or right using the lateral knob on the right hand side Precision forward and backward movements are made with the Coarse and Fine Advance Knobs When turning these knobs you can feel the smooth drive mechanism which allows you to quickly and precisely advance the knife to the specimen Rotating either of the knobs counterclockwise advances the knife towards the specimen Rotating the knobs clockwise causes the knife to move away from the specimen F
14. User Guide for Power Tome Ultramicrotomes Models X and XL Producis by Boeckeler Boeckeler Instruments Inc 4650 S Butterfield Drive Tucson AZ 85714 3403 USA Phone 520 745 0001 Toll Free 800 552 2262 Fax 520 745 0004 www rmcproducts com www boeckeler com info boeckeler com PowerTome User Guide RMC Products Boeckeler Instruments Inc Copyright 2010 by Boeckeler Instruments Inc 4650 S Butterfield Drive Tucson AZ 85714 520 745 0001 No part of this publication may be reproduced transmitted transcribed stored in a retrieval system or translated into any language in any form by any means without the expressed written permission of Boeckeler Instruments Inc PowerTome and RMC Products are registered trademarks of Boeckeler Instruments Inc of Tucson Arizona All other trademarks and registered trademarks are the property of their respective companies P N 020210 ii PowerTome User Guide Boeckeler Instruments Inc RMC Products PowerTome User Guide Thank You for purchasing a Power Tome Ultramicrotome Please fill out the Boeckeler Instruments warranty registration card and fax or mail to take advantage of the one year product warranty provided by Boeckeler Instruments Boeckeler Instruments Product Warranty Boeckeler Instruments Inc products are warranted to be free from defects in materials and workmanship for a period of one year from the date of delivery Boeckeler Instruments
15. ated on a separate control module which can be conveniently placed on either side of the ultramicrotome All of the mechanical components have been carefully isolated from the cabinet and the bench top to assure vibration free sectioning The PowerTome is equipped with all the components and accessories necessary for you to begin using the ultramicrotome except for the knife which may be ordered separately and the specimen This guide has several appendices containing supplementary information e Appendix 1 lists Power Tome specifications e Appendix 2 contains diagnostic routines e Appendix 3 lists items shipped with the Power Tome X e Appendix 4 lists optional Power Tome X accessories with part numbers e Appendix 5 lists items shipped with the Power Tome XL e Appendix 6 lists optional Power Tome XL accessories with part numbers To order additional items contact Boeckeler Instruments in the United States or your local dealer This instruction manual assumes that you are already an experienced ultramicrotomist Since ultramicrotomy is something of an art personal instruction from an experienced ultramicrotomist would be most helpful however there are many books available on the subject Your RMC Products representative can also guide you in the use of your new instrument In addition the staff of the RMC Products Applications Laboratory is available to help you with your specific applications needs PowerTome User Guide
16. bration The section thickness is adjusted using this test This should be performed by an RMC Service Representative only 10 Encoder Test Calibrates the encoder This should be performed by an RMC Service Representative only PowerTome User Guide 27 Appendix 2 Diagnostic Tests RMC Products Accessing Diagnostic Tests Turn the power off at the back of the control unit Depress the top Cutting Window button while turning the power back on Depress the Top Cutting Window button until the desired test number appears in the Cutting Speed Display Depress the Bottom Cutting Window button to activate the test To use another test depress both Cutting Window Buttons simultaneously and then repeat steps 3 and 4 above To return to normal operation turn the power off and then on again 28 PowerTome User Guide RMC Products Appendix 3 PowerTome X XL Accessory Kit APPENDIX 3 POWER TOME X XL Accessory KIT Part Description 311309 3mm Allen Wrench 316749 4mm Allen Wrench 75368 Universal Transilluminated Specimen Holder solid mounting post 75363 Adapter to convert threaded post specimen holders to a solid mounting post 317198 2mm Allen Wrench 315979 2 5mm Allen Wrench 75340 Wrench for Arc Segment Mount 75455 Easy Lift Glass Knife Spacer and Post 75335 Trimming Block Fits onto lower stage Holds P N 75353 Trimming Post or P N 75150
17. cs Reset This button will reset the cutting arm advance mechanism to the beginning of its travel When depressed the small red LED lamp below the reset button will remain lit and the advance mechanism will run in reverse until it is completely reset This will take about 30 seconds during which no button commands will be recognized and the hand wheel cannot be rotated Once the cutting arm has been reset there will be 200um of advance available for sectioning When operating the instrument the advance mechanism continues to advance the cutting arm according to the cutting thickness selected When it has advanced about 190um and there is only 10m of additional advance remaining the small red LED lamp below the reset button will flash and the instrument will beep once each time the hand wheel rotates and a cutting stroke is completed This is a warning to alert the operator that there is only limited advance remaining This will continue until there is no advance left and the instrument will reset itself automatically Advance Totalizer and Section Counter XL only The advance totalizer displays the distance in nanometers the arm has advanced since the display was last reset Select Button Press down and hold this button to display the number of sections which have been cut since the display was last reset Reset Button Press this button to change the distance traveled by the arm to a value of 0 zero Hold down the
18. d aluminum box of 4 threaded post requires P N 75363 Adapter 17005 5 6mm Collet type Specimen Holder red anodized aluminum box of 4 threaded post requires P N 75363 Adapter 17006 4x 5 2mm Flat Specimen Holder red anodized aluminum box of 4 threaded post requires P N 75363 Adapter 75363 Adapter to convert threaded post specimen holders to a solid mounting post 75150 Arc Segment Mount will also accept Leica Ultracut specimen holders 75340 Wrench for Arc Segment Mount 75205 Breath Shield for Knife Stage 75455 Easy Lift Glass Knife Spacer and Post 75353 Trimming Post Fits onto trimming block For solid post specimen holders 75335 Trimming Block Fits onto lower stage Holds P N 75353 Trimming Post or P N 75150 Arc Segment Mount PowerTome User Guide 31 Appendix 4 PowerTome X XL Accessories for reorder RMC Products 32 PowerTome User Guide RMC Products Appendix 5 PowerTome X Shipping List APPENDIX 5 POWER TOME X SHIPPING LIST Item No Description Part No Qty 1 Power Tome X Assy including 75501 1 2 Lower Stage Assy 75637 1 3 Upper Stage Assy 75635 1 4 Power Tome X Controller 12V 75668 1 5 Stereomicroscope SGE 60460 1 6 Breath Shield Knife Stage 75205 1 7 Breath Shield Microscope Housing Assembly 75693 1 8 Dust Cover 75590 1 9 User Guide 051606 1 10 Power Cord 55AH044 1 11 Cable Interconnect 5
19. ed images Figure 1 Powerlome Accessory Box on page 8 e Figure 2 PowerTome XL Ultramicrotome on page 9 Figure 3 PowerTome XL Control Panel on page 10 Figure 4 Upper Stage and Arc Segment Holder on page 11 By referring to these figures as you read you will quickly understand how to control the PowerTome Powering Up The power switch for the PowerTome is located on the back of the control unit When you turn it on the red LED displays and the LED lamps on the control unit will illuminate The red lamp underneath the RESET button will also illuminate The advance mechanism automatically resets to the beginning of travel when the instrument is turned on emitting a faint motor noise PowerTome User Guide 7 Unpack Control Unit Make Connections RMC Products Section 3 PowerTome Basics Accessory Box Photo with Labels 1 Fluorescent Lamp 8 Specimen Holder Adapter 2 Arc Mounting Stud 9 LED Cable Transillumination 3 Allen Wrenches 10 Arc Segment Mount 4 Fuses 11 Specimen Holders 5 Trimming Block 12 Trimming Post 6 Wrench 13 Glass Knife Spacer 7 LED Cable Backlight 8 PowerTome User Guide Section 3 PowerTome Basics RMC Products Unpack Control Unit Make Connections Power Tome XL Ultramicrotome Photo with Labels Figure 2 PowerTome XL Ultramicrotome jan Hand Rests Knife Stage Arc Segm
20. ent Mount Overhead Diffuse Light Stereomicroscope Zoom Knob Focus Knob Microscope Tilt FB forward backward Knob Io I NJa wa KR oO Nw Stereomicroscope Travel Knob an Handwheel Not Shown Tilt LR Left Right see page 16 PowerTome User Guide 9 Unpack Control Unit Make Connections RMC Products Section 3 PowerTome Basics PowerTome XL Control Panel Photo with Labels STEP ADVANCE LIGHTING SYSTEM RESET Gon lt a 0 gt R eo 2 9 i CUTTING SPEED ADVANCE TOTALIZER SECTION THICKNESS MM SEC i NANOMETERS SECTIONS NANOMETERS 00 ODO OO RESET SELECT Figure 3 PowerTome XL Control Panel 1 Upper Cutting Window Limit 8 Memory Storage 2 Lower Cutting Window Limit 9 Program buttons 3 Step Advance 10 Start Stop Automatic Cutting Stroke 4 Step Retract 11 Cutting Speed Selection 5 Overhead Diffuse Light on off 12 Advance Totalizer Reset XL only 6 LED lighting Backlight 13 Advance Totalizer Select XL only Transillumination on off 7 Cutting Arm Reset 14 Section Thickness Selection 10 PowerTome User Guide Section 3 PowerTome Basics RMC Products Unpack Control Unit Make Connections Upper Stage and Arc Segment Holder Photo with Labels Figure 4 Upper Stage and Arc Segment Holder 1 Upper Stage 10 Specimen
21. ething to cut the plastic shipping bands Be sure that the instrument is positioned very near the table counter or cart to which it is being loaded Make sure that nothing is in the way before you begin to lift the instrument 1 Break the plastic bands that surround the container and remove the top 2 Locate the brace inside the container that supports the microscope assem bly It is removeable by breaking the glue joints that secure it to each side of the crate Then rotate it 90 degrees while lifting it out of the container 3 Remove the 8 screws which hold the surrounding casing to the wood bottom 4 Carefully raise the casing above the unit and set it aside The Power Tome is attached to a board that is bolted to the base of the crate 5 Remove the four bolts holding the PowerTome board to the shipping base and lift the board and PowerTome up and place it on your table 6 Using the handholds not the cover lift the front of the PowerTome up high enough for the second person to remove the front bolt holding it to the board 7 Using the handholds not the cover lift the back of the PowerTome up high enough for the second person to remove the back bolt holding it to the board 8 Remove the board from under the PowerTome 9 Place the PowerTome in the location where it will be operated Unpack Control Unit Make Connections Remove the control unit and all other items from the large cardboard box which shipped wit
22. h scan and tilt stereomicroscope 25 Microprocessor contro lissneri ee even A E E EE RE S 26 Dimensi nal dataserie ednini yeni een EEEE S 26 Electrical Data assure aa E EE E EERROR 26 APPENDIX 2 DIAGNOSTIC Erneut 27 Accessing Djasnostie TESS een NE ERPE EE EREE AREEN IREEN EE EEEE Sa 28 APPENDIX 3 POWERTOME X XL ACCESSORY KIT o ccccccssscesseseeseeceseecsseeeesseeessecseeeeseeeecueesessesesaeseeeenseeees 29 APPENDIX 4 Power TOME X XL ACCESSORIES FOR REORDER nun 31 APPENDIX 5 POWER OME X SHIPPING LIST s scscssscccesscsusees nennen ensure eisernen 33 APPENDIX 6 POWER TOME XL SHIPPING LIST ana 35 APPENDIX 7 PT PC INTERFACE MODULE HOOKUP acneei rr EEE NES 37 viii PowerTome User Guide Section 1 Introduction RMC Products SECTION 1 INTRODUCTION All of us at Boeckeler Instruments would like to thank you for purchasing your new Power Tome Ultramicrotome the world s most advanced and user friendly ultramicrotome If the instrument has not yet been unpacked and set up please refer to procedures elsewhere in this manual The PowerTome Ultramicrotome is designed to produce ultra thin sections for electron microscopy as well as semi thin and thick sections for light microscopy A microcomputer controls the internal operation of the instrument but convenient manual controls and easy to operate control buttons give the microtomist complete command ofall functions The controls for automatic sectioning are loc
23. h as air conditioning units and elevator shafts Heavy drafts should also be avoided We realize that ideal locations are not always available so do whatever you can to minimize elements that may reduce the quality of your sections Thoroughly Inspect Containers The ultramicrotome is bolted to a wood base and covered by a wood reinforced cardboard container The accessories are packed in a separate box Inspect both the crate and accessory box carefully for any damage If there is obvious damage notify the carrier immediately Due to the sensitive nature of this precision instrument some damage may not be apparent until after installation and testing At that time it may be necessary to file a Damaged Freight Claim with the carrier Do not discard any boxes or packing material until you are certain you have accounted for all the parts Unpacking Ultramicrotome Crate CAUTION LIFTING THE INSTRUMENT The PowerTome is heavy approximately 91 Ibs 34 kg and should be lifted by at least two strong persons who are experienced in lifting heavy delicate equipment Safety first If you think that PowerTome User Guide 3 Unpack Control Unit Make Connections RMC Products Section 2 Unpacking Installation amp Hookup you and your assistant are not strong enough don t take a chance get more help Tools you will need 1 a 2 Philips screwdriver 2 a 19mm or 3 4 inch wrench 3 a 13mm or 1 2 inch wrench and 4 som
24. h the PowerTome Place them near your ultramicrotome where you will have easy access when you are sectioning See Appendix 7 for PT PC control hookup differences 4 PowerTome User Guide Section 2 Unpacking Installation amp Hookup RMC Products Unpack Control Unit Make Connections Interconnect Cable Installation The 37 pin cable connects the control unit to the ultramicrotome Insert the male end of the cable with pins into the receptacle on the back of the controller and fasten it securely with the two screws which are attached to the cable connector Insert the female end of the cable into the receptacle on the back of the microtome and fasten it securely with the two screws that are attached to the cable connector Attach the power cord to the controller and plug it into an electrical outlet Attach the foot switch if applicable Your Power Tome is now ready for use PowerTome User Guide 5 Unpack Control Unit Make Connections RMC Products Section 2 Unpacking Installation amp Hookup 6 PowerTome User Guide Section 3 PowerTome Basics RMC Products Unpack Control Unit Make Connections SECTION 3 POWERTOME BASICS Before using your PowerTome it is important to become familiar with all the different elements which comprise the system and how they work together to enable you to achieve the high quality sections you desire The instructions in this section are aided by the following label
25. ht The large overhead diffuse light is attached to the underside of the stereomicroscope holder It brightly illuminates your specimen work area Control Panel Elements Sectioning Cut This switch controls automatic cutting under motor power To start or stop automatic cutting press the button once The display above the cut button has 2 LED lamps When the bottom green lamp is lit the cutting arm is in the cutting window of the cutting stroke see below When the top yellow lamp is lit the cutting arm is outside of the cutting window Even when the motor is not running these lamps remain lit to indicate whether the cutting arm is inside or outside of the window Dual Foot Pedal PT XL and PT PC only The foot pedal serves the same functions as the cut and trim buttons on the control unit The right foot pedal switch activates the cut also called ultrathin function The left foot pedal activates the trim also called semi thin function stored in memory location 4 The foot pedal receptacle is located on the back panel of the control unit below and to the left of the on off switch To insert the foot pedal switch into the receptacle line up the red dots and insert Cutting Window These two buttons are for setting the start and end positions of the cutting window Depressing these buttons while the instrument is in automatic cutting mode has no effect The automatic cutting motor must be off to u
26. ine Advance Knob N Figure 6 Fine and Coarse Micrometer Advance Knobs 14 PowerTome User Guide Section 3 PowerTome Basics RMC Products Arc Segment Mount Tip To advance the knife towards the specimen quickly first loosen the Upper Stage Clamping Screw Then using both hands slide the Upper Stage forward to within 1 or 2mm of the specimen Now you can proceed to advance further using the coarse and fine micrometer knobs Caution The lower stage has a maximum of 8mm of travel 4mm towards the specimen and 4mm away There are no physical stops at the ends of the travel When you reach the end of travel in either direction you will encounter increased resistance turning the coarse or fine advance knobs You should then return the stage to its home position with the Coarse Advance knob and reposition the Upper Stage before continuing The Coarse Advance Knob is the larger of the two knobs and has graduation marks that are measured in micrometers um or microns One complete rotation of the Coarse Advance Knob equals 270 um of travel The Fine Advance Knob is smaller and has a white arrow head to indicate the graduation mark position This knob is for very precise approaches of your knife to the specimen It can also be used to rough face your block by advancing a small increment for each cut One complete rotation of the Fine Advance Knob equals 25 um of travel or about 1 0 um per graduation mark Arc Segment Mount
27. ized Person re Warren Brey President r NE ee PowerTome User Guide RMC Products Boeckeler Instruments Inc vi PowerTome User Guide Boeckeler Instruments Inc RMC Products PowerTome User Guide CONTENTS SECTION 15 INTRODUCTION ssiscissscissensatansseceavarveetavenactintansiaceneachicnen er esse een EEEE EENES I SECTION 2 UNPACKING INSTALLATION amp HOOKUD oc ccsssssssesssececessececseseceseseeecssceeecsesaeecssneaesseseaeeseseaaess 3 Carefully Consider LG CACO oceaner rr ener E EEE Ea EERE EEEE 3 Thoroughly Inspect Containers sirien ennie een ee 3 Unpacking Ultramicrotome Grafenau 3 Unp ckControl Unit Make Connections naar 4 Interconnect Cable nenlatian seiner 5 SECTION 3 POWER OME BASICS ae tell ebcedatedestatedesteredssearedece EINE EEEE EE EAEE SE ANETE 7 Powering Up ass kenn ee ie era 7 Accessory Box Photo warn Labels nenne 8 Power Tome XL Ultramicrotome Photo with Labels senssssssssessseessuss snuse sees sees essensen en nsenenee 9 PowerTome XL Control Panel Photo with Labels o ee cc ieccccceccsscccesssescecseccccssecccessueccecaeseeesaseeeeenas 10 Upper Stage and Arc Segment Holder Photo with Labels an aan 11 Lower Stage Photo witht Labels een een ee 12 Power Tome Elements een ee tee veda be fend vnc rr ee 13 Handwheel css Er rn EE EEE E nr ehe een 13 LED Lighting CO een here eran tetaseones eetonnencanabhedeentse osikira ie Ee ie Eees 13 UP Per Stage aan nennen ee 13 Lower S
28. n the down or cutting half of the stroke the arm extends outward for cutting The ample retract of your PowerTome assures that no water will be picked up from the trough by the specimen during the up stroke even when cutting thick sections from large block faces LED Lighting Connections Two cables are supplied in your accessory box for connecting the LED lighting system for either backlighting or transillumination The cable with two connectors supplies power to the light built into the upper stage for backlighting of your knife and specimen during knife approaches The cable for transillumination contains a connector with a light at the opposite end The light is inserted into the side of the specimen holder to illuminate the specimen from behind the specimen block The lower Lighting System button on the control unit is used to turn the LED backlight lamp on and off Upper Stage The upper stage slides directly on to the top of the lower stage and is clamped firmly into position by the black Clamping Knob on the right side of the lower stage When this knob is released the upper stage can slide from front to back This feature can be used to bring the knife to within 1 or 2 mm from the specimen before beginning the final approach The upper stage will also rotate 360 while attached to the lower stage This allows the user to adjust the stage to any position and to view the back side of the diamond knife Power
29. nterface Module Ultramicrotome CR X Chamber PowerTome PC PowerTome User Guide 37 Appendix 7 PT PC Interface Module Hookup RMC Products 38 PowerTome User Guide
30. on Reset Return to normal operation Motor Stop Test Stops the motor and shows the encoder position on the section thickness display Use this test to adjust R12 for no motion Advance Test Resets the advance mechanism to the beginning then advances the mechanism to the end of travel sensor then resets and repeats indefinitely Break In Test Runs the cutting motor and advance motor continuously It will also display the cutting speed in the left display Adjust R2 for 10 mm sec This test is also used to check the operation of the buttons The beeper will sound when any button is pressed The encoder position is displayed in the right display LED Test Lights all segments in both displays and all LED lamps Retract Speed Test Used to set the retract speed Use the buttons under Cutting Speed to select the desired retract speed and then push the Memory Storage button to save the setting Set Defaults Initializes the 4 Program buttons The speed is set to 1 0 mm sec and the section thickness is set to 100 nm The cutting window is set to the upper and lower limits The encoder correction is set to zero and the step calibration is set to one Ram Test Tests the internal RAM and sounds the beeper if no errors are found ROM Test Tests the internal ROM and the beeper sounds if no errors are found The ROM checksum will be displayed on the right display Enter Step Cali
31. prevents the operator from accidentally selecting a thick section which might damage the knife edge It is helpful to set up separate programs for commonly used section thicknesses for ultrathin and thick sectioning The section thicknesses can always be quickly adjusted at any time and stored with the memory storage button or recalled by depressing one of the program buttons Lighting System Overhead Diffuse Light The overhead diffuse light is turned on and off using the top lighting system button on the control unit The top yellow lamp on the control unit will be lit to indicate that this light is turned on LED Lighting Backlight Transillumination Depending on which you ve connected this light is turned on and off using the bottom lighting system button on the control unit The yellow lamp to its left will be lit as an indicator when this light is turned on Step Advance Left Button This button advances the cutting arm towards the specimen by the amount shown in the section thickness display This is very useful for making final approaches of the specimen to the knife Right Button This button moves the cutting arm away from the specimen by the amount shown in the section thickness display This is useful for backing the specimen away from the knife edge if you wish to discontinue sectioning for a moment PowerTome User Guide 19 Advance Totalizer and Section Counter XL only RMC Products Section 3 PowerTome Basi
32. r Specimen Viewing Mirror optional accessory The specimen viewing mirror allows for viewing of the block face during trimming It is especially useful for precision trimming of very small specimens or structures It can help you to monitor sectioning progress through specific structures in your block Figure 13 Specimen Viewing Mirror The specimen viewing mirror is positioned on the upper stage by inserting its base peg into the accessory hole on the top left side of the upper stage the same hole that is used for the water trough filler discussed above The mirror is locked into position using the clamping screw The mirror may be flipped toward the operator during sectioning or trimming and downward away from the operator for viewing the block face The stereomicroscope will only need to be adjusted a small amount to bring the PowerTome User Guide 23 RS 232 Port XL only RMC Products Section 3 PowerTome Basics block face into focus For optimum viewing rotate the handwheel until the block face is one to two millimeters above the knife edge The specimen mirror has been designed so that it should not contact the knife edge of any modern diamond knife However please test your mirror carefully the first time you use it Always be certain that the clamping screw on the left side of the viewing mirror is tightened and the base peg has been inserted into the accessory hole properly RS 232 Port XL only
33. se these buttons To set the cutting window turn the handwheel until the specimen is about 1 mm above the knife edge then depress the top cutting window button to set the upper limit of the cutting window The beeper will sound twice Beep Beep if the position has been accepted Then turn the handwheel until the specimen block face just passes the knife edge Now depress the bottom cutting window button to set the lower limit of the cutting window Again the beeper will sound twice The cutting window is now set PowerTome User Guide 17 Sectioning RMC Products Section 3 PowerTome Basics m Stroke Window Cutting Arm Figure 8 Cutting Window Diagram The computer will not allow you to set a cutting window with the upper position below the lower position or vice versa If this is attempted the computer will beep once to indicate that the position has not been accepted Once the cutting window has been set the cutting arm will slow down to the speed displayed on the cutting speed display as it passes through the cutting window Cutting Speed This button sets the cutting speed in mm sec To change the speed depress the or button until the desired cutting speed is displayed For most specimens embedded in epoxy plastics a speed between 0 5 and 1 5 mm sec will give the best results This speed is in effect only when the arm is passing through the cutting window described above When the cutting arm passes ou
34. t of this cutting zone it retracts at the set retract speed Section Thickness The section thickness in nanometers is indicated in this red display The thickness may be changed by depressing either the or the button One nanometer incremental changes are made by depressing either button once If the button is held down longer it will begin to steadily change the value After 10 seconds the value will change at a rapid rate Remember that 1000 nm is equivalent to 1 0um 1 0 micrometer or micron Memory Storage There are 5 buttons in this area The buttons with the numbers 1 to 4 above them serve to activate any of the 4 stored sectioning parameters These allow you to store section thicknesses and cutting speeds which you commonly use To create a 18 PowerTome User Guide Section 3 PowerTome Basics RMC Products Step Advance program depress one of the buttons The green lamp above the button will light to indicate that the program has been selected Then set the desired section thickness and cutting speed Next depress the memory storage button The sectioning parameters are now stored in the designated program To recall the section thickness in any program simply depress the corresponding button Ifthe section thickness stored is greater than 250 nm the display flashes NOTE If the button is not pressed again within seconds to confirm then the system defaults to the previously selected setting This feature
35. te the knob counterclockwise If water does not pump check PowerTome User Guide 21 Water Trough Filler optional accessory RMC Products Section 3 PowerTome Basics that the pump tube is positioned under the water If water still does not flow remove the five screws which secure the top black plate to the water trough filler body Next remove the pump knob by loosening the set screw with a 2 mm hexagonal wrench and pulling the knob off the shaft During storage and shipment the tubing may have become pinched shut where the peristaltic pump rollers were in contact with the tubing Remove the peristaltic tubing and pump tube The tubing is a very resilient silicone and the pinched areas may be worked open by rolling between your fingers Once you begin using your water trough filler this pinching shut should not recur During reassembly work the silicone tubing back over the rollers while rotating the roller assembly clockwise Pump Knob Reservoir Cover Set Screw LOS III I water N Ds IS Figure 10 Cross Section ofthe Water Trough Filler Positioning Knob x Delivery Tube Figure 11 Water Trough Filler Post 22 PowerTome User Guide Section 3 PowerTome Basics RMC Products Specimen Viewing Mirror optional accessory Silicone Tubing Peristaltic Pump Reservoir O Ring Rollers Filler Tube Figure 12 Open Top View of Water Trough Fille
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