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USER MANUAL - Astec Microflow

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2. BIOQUELL UK LTD 52 Royce Close BIOQUELL ADVANCED BIO SAFETY CABINET ABS CLASS I USER MANUAL TMOI 0 O amp M 001 Tele 44 0 1264 835835 TE Fax 44 0 1264 835836 West Portway Andover Revision 3 Hampshire SP10 3TS United Kingdom Email enquiries bioquell com Page of 61 Web www bioquell com DESCRIPTION i ET Tae T write CE Certifi cale Update and cara m Caution Please read this Manual thoroughly before attempting to use this unit If you do not understand or are unsure of any information contained in this Manual please contact your Agent or BIOQUELL for clarification BIOQUELL designs manufactures and supplies a broad range of containment and airflow products as well as bio decontamination solutions for Rooms Systems and processes Laboratory equipment Biomedical equipment For further information and contact details refer to our web site www bioquell com BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ician Q1 o 44 0 1264 835836 West Portway Andover Revision 3 enquiries IR Hampshire SP10 3TS Page 2 of 61 Web www bloquell com United Kingd CONTENTS MEE FPOREWARD eee ee ee ee T ee 5 1 1 Safety Environmental Conditions ccccccccceececceeceesseeeeeeaeseeeeeesseeeeeeeeeeaeeeeeeseeseeeeessegeeeeeeaas 5 2 INTRODUCTION AND PRINCIPLE OF OPERATION cccccccccccece
3. a Cabinet functions can be customised to the user s requirements b Calibration can be performed remote from the cabinet itself without the need to access electrical components located ina potentially hazardous compartment Executions of both of these tasks are made simple and quick with the use of dedicated set up software obtainable form BIOQUELL 10 2 COMPUTER AND CABLE REQUIREMENTS Equipment required to run the set up software comprises a communication cable and a Personal Computer PC with MS Windows installed and a serial port The PC s monitor should support a screen resolution of at least the VGA standard Physical connection of the computer to the cabinet is now required by means of a suitable cable Serial Cable 9 way male D Connector to 9 way female D Connector Serial Cable 9 way male D Connector to 25 way female D Connector USB RS232 Converter Cable 9 way male D Connector to USB Connector Connection to the controller Cabinet is via the 9 way female D connector located under the front cover on the side of the electrical box The computer end of the cable should be connected to an available Comm port See figure 12 The connector on the computer will thus be either a 9 way male D Connector 25 way male D connector USB Port Note Important The comm port used to connect to the cabinet must be configured as either COM 1 or COM2 BIOQUELL UK LTD TMO10 O amp M 0
4. 10 6 3 Calibrate The calibrate button initiates the calibration cycle which is explained in Section 11 Calibration of Airflow and Alarms 10 6 4 Clear all Clicking on the Clear all button will reset all fields on the setup screen to their default values Commonly default values are zero 10 6 5 Write The Write function will load all current screen parameters to the controller memory BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 48 of 61 Web www bioquell com United Kingdom 11 CALIBRATION OF AIRFLOW AND ALARMS 11 1 INTRODUCTION The setup software Calibrate facility allows calibration of the cabinet airflows and alarms This option will allow downflow and inflow alarms to be set together with the operating speed for the cabinet It is then this speed that the control system will use as a reference and always attempt to maintain The cabinet airflow can be set up to comply with EN12469 2000 or another criteria and the requirements procedures are described in the following paragraphs Note that re circulatory cabinets are set up accurately with a calibrated rig giving accurate inflow and downflow readings Site calibrations will always be less accurate so it is important to double check before altering any settings 11 2 EQUIPMENT REQUIRED e A hot wire anemometer or a 100mm Dia Paddl
5. PVC or rubber gloves which provide suitable protection for the hands and wrists which can be disinfected for re use should be worn when cleaning MSCs At the selection of disinfectants occupational health aspects should be considered 9 2 STAINLESS STEEL COMPONENTS The internal work area s sides back and work tray are made of stainless Steel Liquids or vapours containing Chlorine are known to cause gradual staining of stainless steel To avoid this cleaning agents which containing Chlorine ought to be avoided however if this is not possible then all surfaces should be thoroughly dried and all traces of the solution removed the area should then be treated with a neutralising agent Open vessels left inside the equipment which are likely to cause Chlorine vapours may also result in Staining of the metal surfaces Should the surface of the stainless steel become stained it may be cleaned by mechanical polishing and special treatment of the affected area Note When cleaning to remove bio organisms the method we recommend is to wash with 40 ethanol or equivalent in all poor access points e g corner of liners fold and recesses in the tray It is important to ensure that full Surface contact is achieved and the use of cotton buds is recommended 9 3 PLASTIC COMPONENTS Cleaning of the plastic items should only be carried out with mild detergent or chlorine based cleaning solutions Care must be taken not to apply chlorine sol
6. where X is the number of cycles run with a particular filter The fumigation cycle should be stopped by using the VAP switch and entering the PIN and then the blanking plate ought to be removed and replaced with the spigot Only then the window ought to be unlocked and the cabinet set to normal operation To abort the cycle at any point press the VAP key then enter the PIN and press the Fan button to start the cabinet A description of the cycle is provided in Table 2 Table 2 Automatic Fumigation Cycle ACTION PROCESS DISPLAY MESSAGE Lock shut window Fit blanking plate and pour formalin into vaporiser Press VAP Start Cycle Enter PIN Number Enter four digit Number Vaporiser powered to Evaporation xx mins to run evaporate formalin Vaporiser powered for xx minutes Formalin sterilises inside Fumigating xx mins to run of the cabinet Fan circulates fumigant for Circulating one 5 second phases Fumigation completed Filter fan will start to Purging Xx mins to run purge fumigant through activated carbon filter BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 32 of 61 Web www bioquell com United Kingdom ACTION PROCESS DISPLAY MESSAGE Purge time expired Cycle complete Press VAP amp re enter Cabinet returns to normal WARNING Sc
7. Date of last service and Service Company iv Nature of fault and any other comments likely to indicate cause of fault v A Purchase Order number to cover costs incurred if visit is outside the scope of the Warranty BIOQUELL UK Ltd or other nominated personnel will carry out warranty visits g Inthe event of any health and safety incidents please advise us in writing at the earliest opportunity h This warranty and all other contractual issues shall be governed by English law and the parties agree to submit to the nonexclusive jurisdiction of the courts of England BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 61 of 61 Web www bioquell com United Kingdom
8. FIRST THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IMMIDIATELY LOCAL TO THE EXHAUST OUTLET To purge up the ductwork or temporary vent Open the cabinet s window to its normal working height slowly and lock in position When the window is opened the fan will come on immediately WARNING THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IN THE CABINET CARE MUST BE TAKEN WHILE THE FAN IS STARTING AS SOME PEROXIDE MAY EXIT THE WORKSPACE DO NOT FULLY OPEN THE WINDOW OR INHALE AIR FROM THE WORKSPACE UNTIL SAFE LEVELS OF PEROXIDE HAVE BEEN ESTABLISHED When the cycle on the Clarus L 2 has completed check the level of Hydrogen Peroxide in the cabinet by independent means if below 1 ppm the cabinet is safe to use No external Purge If it is not possible to purge externally the Clarus L2 can carry out the Aeration then the cabinet ought to be left in the same state as for gassing At the end of Aeration by the Clarus L2 the operator ought to check the level of Hydrogen Peroxide in the cabinet by independent means if below 1 ppm the cabinet is safe to use 7 3 DECONTAMINATION WITH FORMALIN Formaldehyde and Formalin are hazardous observe handling procedures Ensure that the cabinet is free of Hydrochloric acid and other Chloro compounds This will avoid the production of the carcinogenic product that results from the mixing of formaldehyde and HCI or Chloride gas The following procedure is provided for guidance purposes only your
9. The following instructions relate to the use of a Peroxide Cabinet and a Clarus L2 A Clarus C can be used refer to Bioquell for details If the unit is not a Peroxide Cabinet the Bioquell EBDS service can still bio decontaminate it with a simple site modification a Check that the cabinet is running in SAFE MODE b Release the window locks and pull the window down the fans will stop working then lock the window shut by use of the two T handles on either side of the window c If the unit is Ducted ensure that it is safe to vent Hydrogen Peroxide through the duct work If the unit is a re circulatory cabinet then blank off the exhaust into the room or attach a vent pipe to the outside for Safe external ventilation or otherwise the Clarus can Aerate on its own although this may take a long time d Connect the hoses large diameter is supply and blue is return and the pressure tube to the connectors on the rear of the Clarus L 2 Connect the other end of the hoses and the pressure tube to the corresponding connectors on the right hand side of the Cabinet as shown in Figure 11 The blue hose connects into the top connection and the large diameter hose in the bottom with the pressure tube connected in the centre Note for the Clarus L2 these connections are required to be 1 5 The cabinet is as standard supplied with 1 connections for decontamination using the BIOQUELL EBDS service Figure 11 Cabinet Hose and Pre
10. Web www bioquell com United Kingdom allowed to ensure that all traces of the material have been removed The following points should be remembered i Although the OES Occupational Exposure Standard may be Satisfied and the presence of the fumigant is not detected by smell the working area of the cabinet may still constitute a hazardous environment towards the material being processed ii Trials with the cabinet have found that concentration levels of fumigant within the cabinet when subjected to purging action exhaust ducted outside laboratory drop rapidly initially but a disproportionate time is required for final reductions to the acceptable level iii A value for this time of 240 minutes has been found to be appropriate to bring concentration level within the currently published OES value 2 ppm However your Safety Officer should be consulted for definite guidance on this and other timer settings suggested 5 Language The messages displayed on the controller screen are in English The option to select other languages is not available 6 Alarms The value at which alarm limits are to be calibrated are entered in the two fields 6a and 6b On execution of a calibration process it is the values entered or previously entered and stored which the user is prompted to set in order that airflow alarms can be recorded by the controller The alarm values are stored as configuration files How to Save and Load configurati
11. and PIN Number Change Keylock This button provides the facility to lock the buttons associated with fan control Then when ever the fan control is to be altered e g turn it OFF or ON the PIN must be entered This is a requirement under EN12469 but the units are shipped without this facility activated see below PIN Number Activation and Change Pressing the button followed by entering the appropriate PIN default 1111 will prompt the user to either Change the PIN or Lock the Cabinet If Lock the cabinet is chosen then it is necessary to press the keylock button and enter the PIN and unlock the cabinet before the fan can be operated 5 2 SLIDING WINDOW AND FAN OPERATION The cabinet has a sliding screen which produces a complete seal when shut This negates the use of a night door when de contaminating To open the Front Sliding Window from the closed position The cabinet will be displaying the following message WARNING Screen locked shut Release the door frame by turning the two black T handles on either side of the window a half of a turn anti clockwise The frame will BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 15 of 61 Web www bioquell com United Kingdom move 20 30 mm outwards It is important the both T handles are turned so that the red dot is at
12. button was not pressed not recommended The Front Sliding Window may also be hinged Figure 5 allowing access into the cabinet for cleaning the inside surface of the window or for loading equipment into the cabinet With the window frame hinged out the window itself can be raised higher To hinge open the widow pull outwards the window frame until it stops then let it back slowly the window will then hold open To shut it again pull the window frame fully open again and it will unlatch allowing it to close BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 16 of 61 Web www bioquell com United Kingdom Figure 5 Window hinged open for increased access and for cleaning Caution Care must be taken to avoid impact damage to the bottom edge of the Front Sliding Window as cracking of the glass may result When wishing to leave the cabinet the fan can be switched off by pressing the fan button recommended or the window can be closed which will also turn off the fan 5 2 1 Integral Bypass Operation The Integral Bypass if fitted works in the same way as described above but also works the room cabinet change over damper as described below Power to the cabinet must be ON If it is switched off or there is a power failure the integral bypass will always shut off the cabinet taking a
13. com Hampshire SP10 3TS Page 8 of 61 Web www bioquell com United Kingdom locked shut ABORTED airflow sensor not connected or greater than maximum limit sensor failure gas solenoid If screen right hand side latched in lower position and the left hand Warning only side unlatched shut LH unlocked If fan ventilation not enabled and the front screen window not shut Warning only and latched in the lower position Window open If service due date exceeded Warning only If battery backed data not retained after power failure Note this would Fan 10096 Fan runs at full denote a loss of all calibration setpoints Battery Fail speed All alarm warning events will be displayed on the LCD as a text message The red alarm indicator will flash and unless muted by pressing the Mute button the buzzer will emit a two tone sound 3 8 LIGHTING Fluorescent tubes provide a lighting level in excess of 750 Lux over the working area 3 9 FILTRATION High efficiency filtration is provided by narrow pleated HEPA filters meeting the requirements of class H14 or higher of EN 1822 1 3 10 CONFIGURATIONS Description Top Box Integral Double Remote Plenum Exhaust Exhaust Exhaust HEPA Fan Filter Required Integral Bypass Integral Bypass Cabinet as Ducted version but with room air intake when cabinet not operating the room air can be either HEPA filtered or straight from the room BIOQUELL UK LTD TMO10 O amp M 001 Te
14. displayed in the drop down menu BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 41 of 61 Web www bioquell com United Kingdom 12 13 3c 3a 3b 15 9 9a s PA CLASS 1 BSC Setup Plocra y n e Ed p PIN nos CommPort Options Messages 10 gt PE 20 03 00 14 33 Em I C 3 months 8 C 6 months ee SS 9 months 4 C 12 months No Change dom v2 02 NN Zi 255 Calibrate Clear 4a 4b 4c 6a 6b 6 Read All Dis Connect Figure 13 Typical Controller Set up Screen 10 4 CUSTOMISATION OF CONTROLS A number of parameters relating to the use of the cabinet can be customised according to individual preference or requirements these consist essentially of timer settings updating of the controller clock and language of the messages and prompts shown on the display The numbers of the following descriptions relate to the features in Fig 13 Note Text in bold found in the following descriptions relates to options found in the set up screen Fig 13 clicking the left mouse button with the pointing device on the symbol its adjacent box or either of its buttons whichever is appropriate will effect a routine or select the appropriate option Controller Clock The controller clock is automatically updated whenever comm
15. fan to operate at full speed This facility is intended for emergency use after a spillage or other inadvertent activity A second press of this button will switch the cabinet s internal or exhaust fan off Alternatively either of the other two fan buttons can be pressed in order to select the desired action 5 1 6 Alarm Mute When an alarm condition occurs the buzzer will sound Pressing this button will cause the buzzer to be muted for a configurable time period of between 0 and 255 minutes See Section 10 Control System Customisation and Calibration The default setting is 4 minutes Cabinets to BS EN 12469 require the default setting at O minutes The intermittent beeping that accompanies FLOW STABILISATION EXPIRED SERVICE DATE messages or attempts to select incompatible conditions are not muted 5 1 7 Test be checked by pressing the TEST button all indicators should Jest Correct operation of button indicators and the audible alarm may light and the buzzer should sound with a continuous tone The TEST function serves the secondary purpose of allowing the HOURS RUN and SERVICE DATE DUE information to be displayed This data is useful in the planning of maintenance operations 5 1 8 Fluorescent Lights Pressing this button turns on the cabinet s fluorescent lighting With power connected to the cabinet the lights can be switched on at any time If UV lamp is in use or th
16. for 1 5 amp 1 8m Gassing Injection rate 4 g min H202 Alert level 100ppm Dwell Time 15 minutes Injection in Dwell No Dwell Injection Rate Can be ignored Total Injection Limit 5 Aeration VFC Delay Can be ignored Aeration Parametric OFF Aeration time 100 minutes H202 Concentration 3 096 Nozzle Alarm Enable OFF If it is not possible to purge using the duct or vent pipe set to ON When Aeration starts it is highly desirable to exhaust the peroxide through the duct work or temporary vent to atmosphere as the time taken to purge will be dramatically reduced CHECK WITH THE HEALTH AND SAFETY OFFICER FIRST THERE WILL BE HIGH LEVELS OF HYDROGEN PEROXIDE IMMIDIATELY LOCAL TO THE EXHAUST OUTLET BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 26 of 61 Web www bioquell com United Kingdom If it is not possible to purge externally the Clarus L2 can carry out the Aeration When it has been deemed safe through independent Sampling of the cabinet s air the Aeration phase on the Clarus L2 can be completed and the cabinet returned to normal Check the level of Hydrogen Peroxide in the cabinet by independent means if below 1 ppm the cabinet is safe to use Running a Workspace Decontamination Cycle a Runa decontamination cycle with the Clarus according to the Clarus manual The
17. hr M hr Drop Pa Drop Pa 1 2m Cabinet 1 5m Cabinet With or without HEPA filter The remote fan must be able to be set at the airflows given Bioquell can supply a fan designed to be controlled from the cabinet The cabinet can control a third party fan which is powered by a single phase 230V motor of maximum power of 400W and is suitable for Phase Angle control Alternatively an inverter drive system can be used as long as it can accept a 4 20mA speed control signal for this an additional board is required to be fitted in the cabinet Where more than one cabinet is using a common duct a Constant Volume Damper will be required and whenever an integral Bypass is installed It is recommended that 160 mm nominal diameter rigid PVC ductwork is used except where the duct work is excessively long or has multiple bends The connecting spigots on both the Cabinet and Fan Box have been manufactured to match this diameter of ductwork so the connections may be made using soft PVC sleeves that slide over the ducting and the spigot and are retained by stainless steel bands NOTE to change the filters the top box plenum has to be raised vertically by a minimum of 20mm as a result the duct work must have a flexible connection to cater for this 4 3 X PREPARATION FOR USE In order to utilise the full operator protection factors given by the cabinet it is essential that the operator is fully familiar with the cabinet and all of its fun
18. is the sole Warranty provided in connection with the product and no other Warranty expressed or implied is provided BIOQUELL UK Ltd assumes no responsibility for any other claims consequential including lost time or profit or other damage whether based in contract tort or otherwise not specifically stated in this Warranty f Except in respect of death or personal injury caused by Seller s negligence or as expressly provided in these Conditions Seller shall not be liable to Buyer by reason of any representation unless fraudulent or any implied warranty condition or other term or any duty at common law or under the express terms of the Contract for any loss of profit or any indirect special or consequential loss damage costs expenses or other claims whether caused by the negligence of Seller its servants or agents or otherwise which arise out of or in connection with the supply of the Goods or their use or resale by Buyer and the entire liability of Seller under or in connection with the Contract shall not exceed the price of the Goods BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 60 of 61 Web www bioquell com United Kingdom Note When requesting a Warranty visit please have the following information available i Product model number and name ii Serial number iii
19. switched on following a maintenance operation involving use of the set up software ii The environment of the cabinet is at the extremes of its operating temperature 10 35 C iii If the cabinets airflow pattern has been recently disturbed In any of these cases the alarm can be expected to clear and after waiting a few extra seconds If an airflow alarm is still displayed after 3 minutes from switching on the cabinet the unit should not be used and advice sought from your Safety Officer or Supplier United Kingdom Email enquiries bioquell com Page 12 of 61 Web www bioquell com D OPERATION 5 1 CONTROL PANEL The touch controls are located on the Control Panel Fig 3 and are described in the following paragraphs eq READ MANUAL BUTTON 1 e 7 L 1 SELECT FAN 9 D o EST 3 ALLOW SYSTEM TO STABILIS a 5 4 F ALARM SOUNDS NOTIFY 8 Y OFFICER Figure 3 Control Panel for illustration purposes only 5 1 1 Mains Power Indicator The blue indicator is lit when the mains supply has been 9 connected to the cabinet The indicator remains lit at all times 5 1 2 Alarm indicator Additionally unless muted by pressing the MUTE button the buzzer emits an alternating two tone signal to give warning of an alarm condition The alarm indicator flashes whenever an alarm condition occurs 5 1 3 Fan Control 2 Operation of this switch causes the cabinets internal or external fan together
20. the top Figure 4 The cabinet will start to alarm with the following message WARNING LH Unlatched and WARNING RH Unlatched Figure 3 Figure 4 Window frame closed Window Frame released Red dot at bottom Red dot at top With both sides released the glass window can be moved using the handles on the glass up to the operating position As the window is raised the fan will start to operate if it was not previously turned off When the glass reaches the operating position a stop will prevent it from being raised any further in addition there is a label indicating where the bottom of the glass ought to be Then push the window frame inwards and turn the two T handles clockwise half a turn to re secure the window onto its seal figure 3 If the window is not at the correct height or the two T handles have not located correctly the following alarm messages will continue to be displayed WARNING LH Unlatched and WARNING RH Unlatched The fan can now be started by pressing the Fan button on the keypad amp will take approximately a minute to stabilise and will display STABILISING FLOW then the following message is displayed CLASS I SAFE stating that the cabinet is safe To close the window carry out the same procedure in reverse turning off the fan first by pressing the fan button The cabinet will alarm when the T handles are released and the fan will stop when the window is shut if the fan
21. 01 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 40 of 61 Web www bioquell com United Kingdom Electrical Box Comm Port 1 or 2 Communication 259 0990 Cable RS232 Port a b or c above with Amplifier 259 0994 256 5435 1 Lap Top Computer Class 2 Controller ABS 4 Way Cable Running PCB Assembly Assembly V1 00 Software For Ducted or Re circulatory Cabinets Figure 12 Communication Cable 10 3 INSTALLING THE SET UP PROGRAM To install the BSC Set up program on the computer use Explore and install the program by selecting the file named Setup in the same way that other programs are installed To check if the operating system installed on the computer is compatible with the program refer to BIOQUELL After completing the installation an icon will be created Double click on the icon a computer terminal labelled BSC to run the Set up programme The screen shown in Fig 13 will be displayed The screen layout has been designed for use with a variety of screen devices for this reason it should always be used in full screen mode or it is possible only part of the set up blocks will be visible When starting the SET UP programme should the error message COM 1 or COM 2 Unavailable appear Click comm port See section 10 5 3 on the top menu bar and select the opposite to message
22. 5 Wait until this clears if this fails to clear within 10 minutes there is a problem with the flow sensor as it is not stabilising seek technical assistant FABSC Setup Program V 2 40 File PlNnos CommPort Options Messages Languages Serial No Calibrate Flow Control bee c Please Wait For Stabilisation 23 02 m 23 02 01 JB 00 01 3 months C B month 9 month C 12 month C Disable No chant do v1 92 Zantinue Main Fan fri 51 rfj 163 Exhaust ud Start E BSC Setup Program BY Microsoft Word Do qm 14 57 Figure 15 Please Wait for Stabilisation Dialog Box BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 51 of 61 Web www bioquell com United Kingdom 12 1 6 High Inflow A new dialogue box will now appear similar to Fig 15 but with 1 0 m s as the value of velocity the instruction specifies to set will be the value retrieved from the configuration file With the anemometer at the position defined in 11 4 1 adjust the fan to give the value of 1 0 m s 198 ft min Should the voltage to the fan exceed 195 V ac it is recommended to NOT to set above this figure Dragging the slider next to Main fan speed title on the sub screen makes adjustment Alternatively for small increments the bar either side of the slider may be cl
23. ABINET MAINS POWER CONNECTION Mains power connection to the cabinet is made by the use of a standard IEC 3 pin connector assembly incorporating dual pole fusing and an isolator switch Fig 6 r E E Figure 6 Mains Connector and Switch BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 18 of 61 Web www bioquell com United Kingdom 5 5 SAFETY CONTROL FEATURES 5 5 1 Automatic Air Velocity Control The control system automatically compensates the airflow for the dirtying of filters and variations in the mains power supply Whenever the airflow is within safe operating conditions the display reports the message CABINET SAFE If an alarm condition is detected the buzzer will sound and the alarm LED will flash the cause of the alarm will be reported on the display After switching on the cabinet from cold an airflow alarm may occur for a short period This is due to the stabilisation of flow sensors and airflows During stabilisation the display shows the message STABILISING FLOW 5 5 2 Automatic Fan Start After Power Failure In the event of power disconnection from the cabinet while the fan is operating reconnection of the power will cause the fan to start However all other functions will be automatically set to off and if required they must be reselected If F
24. FF AND DISCONNECTED FROM THE POWER SUPPLY The four nuts holding down the top box or exhaust plate which is located on the top of the cabinet must be removed Lift off or hold up the top box or exhaust plate Remove the screw at either side of the cabinet which holds the front cover down Then lift the front cover and two teleprops on either side will hold it open Lower the front sliding window to the closed position The exhaust filter s are large panel filters located just above the work area After replacing the filter put the top box back on and by clamping it down the exhaust filter will be sealed in position Lower the front cover by pushing it up allowing the tele prop to unlatch Secure the front cover with the two side screws 8 5 FLUORESCENT TUBE REPLACEMENT WARNING The CABINET MUST BE SWITCHED OFF AND DISCONNECTED FROM THE POWER SUPPLY Remove the screw at either side of the cabinet which holds the front cover down Then lift the front cover and two teleprops on either side will hold it open BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 36 of 61 Web www bioquell com United Kingdom Remove the ends off the tube by simply pulling away and then unclip the tube from the holders Place the new tube in the holders place the end on and fit down and secure the front cov
25. IT O d 49 11 2 Equipment Required a cane crt sarees crasso esiumaMteieteqiteinau s adetduniat dd inte xnu edel asau eniti IR RUNE 49 11 3 Pi lgie dure MINE TETTE 49 11 4 Calibration ml ce let 9 0 ee een OU 49 11 4 1 Initial Set up and measurement of inflow eee cceeeecneeeeeeeeeeeeeeeeeeaaaeeeeeesaaeeeesesaeaeeees 50 ar LOVIN EE 50 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 4 of 61 Web www bioquell com United Kingdom FOREWARD This manual has been specially prepared to give guidance in the use and maintenance of the MICROFLOW CLASS I ADVANCED BIO SAFETY CABINET ABS Mk II WARNINGS 1 USE OF THE CABINET IN A WAY OTHER THAN DESCRIBED IN THIS MANUAL COULD PROVE TO BE VERY DANGEROUS 2 GERMICIDAL LAMP EMITS UV RADIATION 253 7 nm EXPOSURE WILL CAUSE DAMAGE TO SKIN AND EYES VACATE AREA WHEN IN USE A list of Safety Symbols used on the Cabinet labels is provided in Section 17 In order to utilise the full operator protection factors given by the cabinet it is essential that the operator is fully familiar with the cabinet and all of its functions and controls This manual should be thoroughly studied before the cabinet is used This manual contains the operational and first line maintenance instruct
26. RES LIST AUTOMATIC FUMIGATION SYSTEM BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 55 of 61 Web www bioquell com United Kingdom 14 SERVICING RECORD DATE ACTION COMPLETED BY BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 Email West Portway Andover enquiries bioquell com Hampshire SP10 3TS Page 56 of 61 Web www bioquell com United Kingdom 15 EC DECLARATION OF CONFORMITY EC DECLARATION OF CONFORMITY FOR ATTACHMENT OF CE MARK Ce We the manufacturer BIOQUELL UK Limited 52 Royce Close Portway Industrial Estate Andover Hampshire SP10 3TS Tel 44 0 1264 835835 Fax 44 0 1264 835836 Declare under our sole responsibility that the appliance ABS CLASS Microbiological Safety Cabine Model Numbers 1000 Serial Number as stated on Certificate of Factory Testing Complies with the Protection Requirements of the Electromagnetic Compatibility Directive 2004 108 EC and the Machinery Directive 2006 42 EC by means of the following harmonised standard BS EN 61326 1 2006 Tested By TRaC Holly Grove Farm Verwood Road Ashley Ringwood Hants BH24 2DB Certificate No 5790 04 Certificate No 6622 09 Other harmonised standards used EN 61010 2001 i Responsible Person Na
27. Safety Officer should be consulted for definitive guidance Formaldehyde penetrates poorly and its effectiveness is dependendent on temperature and humidity It is most effective above a temperature of 20 C and a relative humidity of 6596 Use of excessive amounts can result in polymer deposition within the cabinet and may contribute to filter blockage 7 3 Fumigation with an Integral or Separate Fumigation Unit If a stand alone separate fumigation unit is used which is then plugged into the internal socket the same procedure can be followed as described below but the vaporiser unit will have to be turned on manually BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 28 of 61 Web www bioquell com United Kingdom A safe manner to exhaust the fumigant is required the following are a list of common methods Use the existing Ducting for permanently ducted cabinets Fit temporary ducting to a central duct or other exhaust system Fit a Formalin Evacuation unit to the exhaust a small portable unit designed specifically for this purpose available from BIOQUELL i Use an in built filter for removing the vapor only fitted to Option K versions of the cabinet refer to section 7 2 2 The cabinet display prompts the user throughout the fumigation cycle A safety feature built into the cont
28. Tm 14 S MEE Eo die daMbancctddemicedtesumetsadinaientsa 14 AO APOS aO OVCE 15 k GaS OO r i E E E E S 15 5 1 12 Keylock and PIN Number Change ccccccssssccccceeseeceeeeseeeceeeeeeeeeeeeseeeeeessseaeeeeessaaaes 15 5 2 Sliding window And Fan Operation aes soccuteken und en secca uer Opa 0o Chio iacu na duo soie pues Oeo ep enteras 15 5 2 1 lntedral Bypass ODGOLalODlussesadeo iet audeo teen enden secco tiec sUpu aes Sedit uo cuota des ceci pon E 17 D WORK MIRI o MEER Eom 18 5 4 Cabinet Mains Power Connection ccccccccssssecececeesseceeceeesseceeeeeeeceeseeeeeeceeeeeeaseeeessaaseeeess 18 5 5 Safety Control Features cccccccccccssssseeeccceeeeeeeeseeeeceeeeseeeeseeeceeeeeseeeeseeeeeeeessuaaaeeeeesesssaaaaesss 19 5 5 1 Automatic Air Velocity COntrol ccccccccccssseeceeeeeeeeeeeeesseeeeceeesseeeeceeessaeeeeeeessaaeeeeessaaees 19 5 5 2 Automatic Fan Start After Power Failure ccccccccccseseeeceeeeeeeaeeeeeeeeeeeeseaeaeeeeeeeeeessaas 19 5 5 3 Hour Meter and Service Date Due eesssssssssssssseeeeeeeenenen nennen nnne nnn nnns 19 JNEMES 201075 40 7 c E 20 6 1 Solenoid Operated Gas Inlet cee cccccccsssseeeeceeeseeeeeceeeeeeeeeseeseeeesseeaseeeesseaeeeeessaeeeeesssaeses 20 02 MANS SOCKET MERE 20 693 DENS TAD ane ene ee ee ee E aR 20 6 4 Hydrogen Peroxide Connections ccccsscccccssscecceesececeeueccccaueeeeceueeeessageeesseuse
29. arms at the window access at the front if none Mute alarm switch off cabinet and contact service provider Replace Filters Mute alarm switch off cabinet and contact service provider Screen LH or RH Check left and right hand latches have engaged unlocked correctly adjust if required Check magnets are in position Check screen is at working height if screen is above or below working height marks screen stop may require adjustment located behind main front cover Fan 100 Battery Fail No Gas supply Check control panel display is indicating SAFE If it is switch off cabinet and contact service provider UV Light not working Check magnet is located on front screen and is in Portable line with switch on UV light unit Check light unit is positioned correctly onto front grill Fixed service provider service provider reset PIN BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 54 of 61 Web www bioquell com United Kingdom 13 SPARES Refer to the Supplier or the Maintenance Provider for a comprehensive list of Spares Below are the most common parts requested MODEL ABS 1000 ABS 1200 ABS 1500 Main Filter MSABS1000 MSABS1200 MSABS1500 031 031 031 Integral Bypass Filter MSABS1000 MSABS1200 MSABS1500 030 030 030 ITEM QTY USED DESCRIPTION PART No Wan Fan 230V ecra SPA
30. ble consult your Health and Safety Officer The new carbon will be supplied with a sealable bag in which the used carbon filter should be put as it is removed from the cabinet 7 4 GASSING THE LABORATORY Should this be required at any time full information for UK organisations is given in the Department of Health Memorandum Control of Outbreaks of Small pox 1975 and advice should also be taken from your Safety Officer BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 33 of 61 Web www bioquell com United Kingdom Laboratories can be decontaminated using Hydrogen Peroxide with a Clarus generator or using the BIOQUELL Room Bio Decontamination Service RBDS Alternatively Formalin may be used but this methods has a significantly longer down time and associated additional hazards It should be noted that Formaldehyde vapour in normal dilutions will not affect any part of the cabinet or its controls Note It is the responsibility of the user to ensure that whatever means of cabinet decontamination is used it is compatible with the material being handled and has been approved by the Safety Officer BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Pag
31. ce company 8 3 ANNUAL MAINTENANCE TESTING AND RE CALIBRATIONS It is recommended that the annual service visit is carried out by BIOQUELL or its local distributor The service shall consist of the following a Visually check the cabinet internally and externally for surface defects cracks or other damage Check the window and its operation and seals b Extract duct work to be examined where practicable for defects cracks or other damage if fitted and that it is clearly labelled C Check the anti blow back if fitted is functioning and clearly visible BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 35 of 61 Web www bioquell com United Kingdom d N All alarms to be checked and tested and re calibrate the exhaust airflow sensor if required e Filter and seal integrity checked using DOP If there are two exhaust filters they ought to be checked independently f Air inlet velocity g Pre filter changed if fitted h i KI discuss test is recommended N 00 Full function and keypad test 8 4 FILTER SERVICING WARNING FILTERS MUST BE DECONTAMINATED PRIOR TO REMOVAL WHEN REMOVED THEY MUST BE BAGGED AND DISPOSED OF IN A SAFE MANNER It is recommended that this done by a trained service provided 8 4 1 Exhaust filter s WARNING The CABINET MUST BE SWITCHED O
32. ctions and controls This manual should be thoroughly studied before the cabinet is used When commencing use the following procedure must be followed for safe operation abbreviated instructions are shown on the Control Panel BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 11 of 61 Web www bioquell com United Kingdom a b c d e f BIOQUELL UK LTD TMOI 0 O amp M 001 Tele 44 0 1264 835835 TE Fax 44 0 1264 835836 West Portway Andover Revision 3 52 Royce Close Hampshire SP10 3TS Plug in the power lead if applicable Switch on power at the switch located at the power entry point Confirm that the blue LED on the control panel is lit indicating that mains power is applied to the cabinet Press and hold down the TEST button All LEDs on the keypad should light and the buzzer should sound If not the cabinet should not be used and maintenance advice sought Open the window to operating position see section 5 2 and press the FAN button if prompted enter PIN number The fan will take about 1 minute to stabilize during this period the alarms are muted It is possible that after the stabilising process the buzzer sounds and the display advises of an airflow alarm The possible causes are as follows i The first time the cabinet is
33. dicate the position of the switch if the UV light does not work when expected adjust its position slightly The fluorescent lights must be switched off Unless these lamps are off it will not be possible to switch on the UV lamp The U V light is operated by use of the U V light switch see section 5 1 9 6 11 3 Remote Start Stop Switch A remote start stop switch can be supplied to be fitted outside the room where the cabinet is fitted This key switch requires turning momentarily to initiate the stopping of the fan and starting it replicating the fan button Note that the window on the cabinet must be in operating position otherwise the cabinet will alarm and not operate Note if an Integral Bypass is fitted the Fan time delay button must be used to switch to the room air as this allows the window to be left open without the alarm sounding If turned off at the remote keyswitch the alarm will not sound either BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 23 of 61 Web www bioquell com United Kingdom 7 DECONTAMINATION OF THE CABINET It is recommended that the cabinet be kept clean and free of dust by swabbing down or washing down with a suitable disinfectant Disposable gloves should be worn for this task Do not use any Chlorine based cleaning agents The cabinet should be kep
34. ds on the set up screen b Click on File followed by Save c Type a path and file name in the File Name box For easy management the choice of a file extension cfg is recommended this will store all files of this type together and will display them each time a Load Settings selection is to be made d Click on OK If a configuration file is to be used on more than one cabinet it should be saved on the computer s fixed disc Thus the drive name and directory path should prefix the file name e g C SETUPS FILENAME CFG SETUPS is a directory specifically created for the storage of configuration files and FILENAME is a name for the file which facilitates easy identification e g BS_12 10 5 1 3 Exit The Exit option can be used as an alternative to the close window button to terminate running of the set up program 10 5 2 PIN Nos Maintenance of the PIN numbers can be carried out by selecting PIN Nos The PIN numbers are the four digit codes which allow the fan selections to be locked and also allows use of the vapourisation cycle to those persons with knowledge of the applicable number When the Connect operation has been executed the following two options are available 10 5 2 1 Read Allows the stored codes to be interrogated and displayed in a box on the screen BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enq
35. e made 10 5 4 Options Selecting Options allows enabling of the following two facilities 10 5 4 1 Direct Control When selected the fan speed slider area 9 of Fig 13 can be used to directly adjust the Exhaust fan speed This is useful primarily during the calibration process described in the next section 10 5 4 2 Show Sensors When selected numerically scaled sensor output values are displayed in area 7 of Fig 13 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 47 of 61 Web www bioquell com United Kingdom 10 6 MISCELLANEOUS FUNCTIONS 10 6 1 Dis Connect This button serves both the functions of connecting and disconnecting the communication link between the PC and Controller The current status of the communication link between the PC and the controller is indicated by the graphic on the Dis connect button When the communication link is disconnected the two connectors are shown separated when connected they are together 10 6 2 Read Although all setup parameters are automatically read from the controller and displayed on the screen whenever a Connection is made Read will repeat this function Read is therefore used after a variable has been changed and written to controller memory for example and it is subsequently required to check the controller s current settings
36. e retrieved from the configuration file With the anemometer at the position defined in 11 4 1 adjust the fan to give the value of 0 7 m s 140 ft min Adjustment is made by dragging the Fan Speed Slider on the calibrate screen Alternatively for small increments the bar either side of the slider may be clicked or for the smallest adjustment either direction arrow may be Clicked BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 50 of 61 Web www bioquell com United Kingdom CLASS 1 BSC Setup Program V 1 00 PIE File PIN nos Comm Port Options Messages Language SenalNa MICROFLOW ME L alibtrate Flow Control ER Contamination Control 20 03 00 14 34 Position anemometer in an appropriate position to set the low inflow alarm Adjust the fan speed slider until a velocity of 0 70 m s is shown on the anemometer C 3 months C B months 5 9 months C 12 months No Change lin n L ancel Figure 14 0 7 m s Low Inflow Alarm Setting When the correct speed has been obtained as indicated on the anemometer although the software waits until sensor values are stable it is advisable to observe the sensor values until the numbers are steady before clicking Continue Click on the Continue button A new dialog box will appear Please wait for Stabilisation see Fig 1
37. e 34 of 61 Web www bioquell com United Kingdom 8 MAINTENANCE The Biological Safety Cabinets have been designed to give many years trouble free efficient service and to keep maintenance to a minimum However to ensure this they must be regularly cleaned and checked A full maintenance service is offered by BIOQUELL Overseas this same service is offered by the BIOQUELL distributors In addition to this however operators should carry out the following procedures on a schedule agreed with the Safety Officer so as to ensure that the cabinet is always in first class working order 8 1 DAILY FREQUENTLY a Keep the unit clean externally as well as internally It should be wiped over with a damp cloth using a proprietary bactericidal solution The front viewing panel should be kept clean so that the operator always has a clear view of work being performed inside the cabinet b Ensure that the audible alarm and all indicators are functioning by pressing the TEST button Any faults should be corrected before the cabinet is used 8 2 WEEKLY MONTHLY In addition to the above the following checks should be carried out a Visually check for damage to the seal on the front of the cabinet which is compressed by the glass of the front window b Inspect all mains cables to the cabinet or to any extras that may have been fitted for damage or wear In the event of any fault being discovered contact a BIOQUELL approved servi
38. e UV lamp switch has been pressed UV lamp switch LED lit it will automatically be switched off when the fluorescent light is selected 5 1 9 UV Lamp WARNING DUE TO ITS HAZARDOUS EFFECTS THE CABINET OPERATOR SHOULD AVOID ANY EXPOSURE TO UV RADIATION BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 14 of 61 Web www bioquell com United Kingdom When selected the UV lamp will remain on for a configurable time period after which it will automatically switch off The default setting is 15 minutes The time period for which the UV lamp remains on can be changed see Section 10 Control System Customisation and Calibration 5 1 10 Vaporisation Cycle JAC JA 5 1 11 Gas Supply rm As a safety feature in series with the gas supply tap is a solenoid e Pressing this button initiates the decontamination process See Section 7 Decontamination of the Cabinet for the procedure valve which is controlled from the control panel Thus the gas supply can only be selected if the airflow is in a safe condition i e no alarms are active Once the valve has opened gas supply can be controlled via the tap inside the cabinet in the usual way Caution The solenoid valve will automatically close the gas supply if an alarm occurs during the time it is selected 5 1 12 Keylock
39. e UV light requires altering this can be easily done through the maintenance software refer to your local distributor or alter with set up software as described in section 10 4 Customisation of Controls WARNING DUE TO ITS HAZARDOUS EFFECTS THE CABINET OPERATOR SHOULD AVOID ANY EXPOSURE TO UV RADIATION BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 22 of 61 Web www bioquell com United Kingdom 6 11 1 High Level U V Light The high level U V light is permanently fitted and is operated by use of the U V light switch see section 5 1 9 As a safety feature the internal fluorescent lighting must be switched off and the window shut prior to switching on the U V light otherwise it will not operate 6 11 2 Portable U V Light Place the Portable U V light at the front of the cabinet positioned centrally along its length The power lead can then be connected to the socket labelled UV on the internal wall of the cabinet Two safety related interlocks are incorporated into the circuit powering the UV lamp The front viewing panel must be fully closed thereby achieving the alignment of a magnet on the window with a reed switch in the rear of the lamp casing If alignment of the two components is not achieved the lamp will not function A series of holes on the rear of the lamp casing in
40. e wheel anemometer can be used e A Laboratory stand to hold the anemometer e Multimeter e Laptop Computer with V1 00 Calibration software Installed e Communication Cable see section 10 2 11 3 AIRFLOW REQUIREMENTS The inward airflow velocity through the working aperture shall be between 0 7 and 1 0 m s 11 4 CALIBRATION PROCEDURE Calibration ought only to be carried out by a suitably trained Technician The Calibrate process will instruct the operator to set the airflow via a virtual slider on the screen to the two alarm velocities of low and high In Flow These values will then be used by the set up software to derive an operating condition for the cabinet such that no alarms will be activated When the data is written to the controller the values will be stored and used until the cabinet is next calibrated or adjusted The operating condition of the cabinet can be manually adjusted if desired To calibrate the cabinet proceed as follows If communication between the computer and controller is not made click on Dis Connect area 11 Fig 13 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 49 of 61 Web www bioquell com United Kingdom It is not unusual to see a dialog box saying Controller not configured correctly please configure Disregard this message as the following
41. ececeeeeeesesseeeeeeeeeeeeeeeeeeesseaaaes 6 2 1 Responsibility ofthe USE TET 7 7 SPECIFICATION oee E E EEE 7 SA Operating EF IVAGO AVG Misasi anA EAE nei a Ea E EEEE STERED 7 SS SOS EIGN OIA ee E E NE EE EEE E EE E ETE 7 SC MESI esiii im aa aE Eia Ea RANEES 7 s E E MEE 7 SOME Duil oP c 8 39 6 Nondeturm Valves DUCTCO uavecsvisavaex idt vixsPA RR EDT YesU DU OPE REEPFbeU Vid uet addi pex EdnUvaN a dU iranin aisits 8 STEM cic 8 SEEN Roue m u 9 CS EE lucu OM ERE T 9 3 10 CONFIQUIATIONS MEME 9 LMMMI SCIES EN 30 MwTT 10 4 1 J Position of the cabinet seessssssssssssesseseseseee nennen nennen nnne nnns nn nnns nnne nnn 10 4 2 Position of the Fan for Ducted Cabinets ccccccccccccccssssseeceeeeeeesseeeeeeeeeeeessueaeeeeeeeeeessaaaaeees 10 4 3 Preparation For USe iiii entire boit bn t bnt ana EFE RII qain PRI IIS KR PAM L PKRRA EE Pte pA FOR ERIA a dRRE 11 B9 1 0 E HW eee 13 SM CONTORNO mmm 13 5 1 1 Mains Power WANS A DPussscassoism t perintas EE rIER Xue d Rene tnu ca un nad trtn ide ripis ut detuutu E nia CES 13 Sm P Aam NOLA cn Rm 13 a Fanm OnO e E E A A 13 2x Fanm OME r E E E E AE E E E E E E E 13 ebo FPI ee E E E EE 14 XN AN G ee a a A E EE E A A E EES 14 r TE c 14 5189 esed ec
42. eeeeees 28 7 3 2 Automatic Fumigation Option K ccccccccssseseecceeeeeeeeeeeeeeeeeeeeeaeeeseeeeeeeeseseeaeeeeeeeeessaas 31 7 3 2 1 R nnng an Automate CCN H 31 7 3 2 2 Carbon MSG eal NN Cle x setae 2s tee boton iria c u ere Sind dese Dette bec deen qe dep acess nee 33 74 Gassing the Laboratory eR inik serieak Eai Kedi Naa bek aiK neiaa nE Enia EENE ND KNEA 33 OA sine 35 o1 Daly Freguemlly PTT 35 MEME Iuuen ee E A E A E 35 8 3 Annual Maintenance Testing and Re Calibrations ccccccccccccssseeeeeeeeeeeeeeeseeeseeeeesaeeeeees 35 oA u qpe siettMPTE T Em 36 8 4 1 AUS ilic P 36 8 5 Fluorescent Tube Replacement ccccccccccesssecececeeeeeceeeeeeseeeeeeeeeueeeeseeaseeeessaaeeeeessaaeeees 36 86 UV TDS Replacement ERE mE 37 MM OCUPO OCIO CR E E ne ee ee eee eae eon ee eee 37 MESE PISIS 38 9 1 Bio Organism Removal Cleaning ccccceccccccseseeceeeeseeeeeeeceeeeeeeesseeeceeeeseaeeeeesseaeeeeeessageeeees 38 9 2 Stainless Steel Components ssssssesssssssssssseeeeee nenne nnnnnnnn nnne ness sn nna i nnn nnns sane nain nns 38 NAS WCC OU ON SIS cde ea PECES TOR 38 9 4 Painted and Other Components cccccccecccccceeeeceeeeeeeeceeeeueeeeeeeeeseeuceeeeseaeeeeeessaageeeeessageeeees 39 10 CONTROL SYSTEM CUSTOMISATION AND CALIBRATION
43. eeseuseessseseeeees 20 65 FONTAN Flore i UE mmt 20 CO i o GaS BUE Emm 21 6 7 Touch o matie Gas BUET ecce rre etat erar tI ambi ede aa e Re pol one Ra SIRE ntn cia ERE eei deiina NEARER 21 o 0 HN c 21 C WOME PRG OI ACU M 21 6 10 Internal Socket Residual Current D VICC ceeccccccccecccaeeeeeeeeeeeeeeeeeeeeeeeeeeessaeaeeeeeeeeeeeeaas 22 6 11 9n wE O 22 6 11 1 High Level U V Light nnne nnnm nnnm nnnm nnn nnns 23 6112 Portable UV LIONI NNHE 23 6 11 3 Remote Start Stop Switch 0 00 cccecccceceeeeeeeeeeeeeeeeeeeeseeeeeeeeseaaeeeeeesseeseeeeesseeseeneeeas 23 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 3 of 61 Web www bioquell com United Kingdom 7 DECONTAMINATION OF THE CABINET 0 0 0 0 ce cece ee eee eee e ee eeeeceeeeeeeeeeeeeeeeeeeeaaaaaaaaaceeeeeeeeeseeeeees 24 7 1 Sealing of Re Circulatory Cabinets Prior to Decontamination ccccceeeeeeeeeseeeeeeeeeeeeeeaes 24 7 2 Decontamination with Hydrogen Peroxide sees 25 7 3 Decontamination with Formalin cccccssecceccsescceceeseeeeeeeeeeeseseeesseeeeesseeeeeeseeeeeesseeeeesseeeeens 28 7 3 1 Fumigation with an Integral or Separate Fumigation UNit ccccecccseeeeeseeeeeeeee
44. en Class I window BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 29 of 61 Web www bioquell com United Kingdom The operator ought to open the window or if a Formaldehyde Extraction Unit is being used switch it on first for 10 to 15 seconds and allow the unit to run for the purging time Periodically check the room with a formaldehyde meter this should read less than 2 ppm formaldehyde Purging will continue until the purge time has expired The cabinet will then return to normal cabinet functions Remove the temporary duct Evacuation unit close the orange valve first as appropriate and run the Cabinet as normal and check the room with a Formaldehyde Meter A description of the cycle is provided in Table 1 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 30 of 61 Web www bioquell com United Kingdom Table 1 Fumigation Ducted Cycle ACTION PROCESS DISPLAY MESSAGE Lock shut window and Start Cycle Enter PIN Number pour formalin into vaporiser Press VAP Enter four digit Number Vaporiser powered to Evaporation xx mins to run evaporate formalin turn on manually if unit is not integral Vaporiser powered for xx minute
45. en actually needed and so causes minimum disturbance to the airflow pattern within the cabinet 6 7 TOUCH O MATIC GAS BURNER This is a gas burner which is operated by a palm control The control is linked to the gas burner and operates a valve to allow passage of gas a pilot flame in the burner ensures immediate combustion of the gas The flame is extinguished by the release of the palm control and its size and intensity can also be varied 6 8 STAND For applications where bench space is not available for the cabinet a stand is available with either adjustable feet or swivel castors The height of the stand can be adjusted between 675 and 850mm in 25mm increments at installation to suit the operator This gives a work surface height of 745 to 920mm In addition there is a foot rest at the front which can be moved to also suit the operator 6 9 VOLT FREE CONTACT An option is available so that continuous remote monitoring of cabinet operating status can be performed Interfacing to the cabinet is via a multi way plug located on the top surface of the cabinet Parameters which affect the closing of relay contacts are a Cabinet fan on b Airflow alarm active BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 21 of 61 Web www bioquell com United Kingdom Contacts are rated at maxi
46. er 8 6 UV TUBE REPLACEMENT To change the tube pull off the end fittings unclip the the tube from the the holding clips Place the new tube in the holder by doing the above in reverse order 8 7 CIRCUIT PROTECTION There are two fuses located next to the power entry socket Note the 120V version does not have any fuses but an overload trip instead To gain access to the fuses push the little tab in and pull the drawer up and out The two fuses are attached to the drawer Replace always with T10 Amp fuses Changing of any fuses on the control board must be carried out by a trained technician BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 37 of 61 Web www bioquell com United Kingdom 9 CARE AND CLEANING 9 1 BIO ORGANISM REMOVAL CLEANING Special attention for cleaning must be given to the door seal Thoroughly clean with disinfectant then rinse well with sterile water All MSCs should be kept clean and free of unnecessary equipment The interior should be swabbed after use with a suitable disinfectant Phenolics quarternary ammonium compounds and aldehydes can be use for disinfecting these surfaces Users of chlorine compounds should be aware of their corrosive nature The use of alcohols should be undertaken with caution because of the risk of fire Heavy duty polyvinyl chloride
47. following cycle parameters ought to be set for bio decontamination of the workspace Remote Start Pressure Test Only OFF OFF Test Pressure Test Time Test decay can be ignored Aeration Only Pressure Control Aeration Pressure Control Pressure Setpoint Pressure Low Alarm Pressure High Alarm Airflow setpoint Delivery Temperature Conditioning Parametric Conditioning Time Gassing Time Gassing Injection rate H202 Alert level Dwell Time Injection in Dwell Dwell Injection Rate Total Injection Limit Aeration VFC Delay Aeration Parametric Aeration time H202 Concentration Nozzle Alarm Enable OFF ON OFF 10Pa 80Pa OPa 20 m3 hr 60 C OFF 10 minutes 8 mins 4 g min 100ppm 10 minutes No Can be ignored 5 Can be ignored OFF 60 minutes 30 OFF If it is not possible to purge using the duct or vent pipe set to ON b With the window locked down start the Clarus L2 cycle c After 28 minutes the Clarus L2 will be starting Aeration it is highly desirable to exhaust the peroxide through the duct work or temporary vent to atmosphere by use of an Option B or C as the time taken to purge will be dramatically reduced BIOQUELL UK LTD 52 Royce Close West Portway Andover Hampshire SP10 3TS TMOI O O amp M 00 Tele 44 0 1264 835835 Revision 3 Fax 44 0 1264 835836 United Kingdom Email enquiries bioquell com Page 27 of 61 Web www bioquell com CHECK WITH THE HEALTH AND SAFETY OFFICER
48. has a maximum permitted earth leakage of 0 5 mA 6 3 SERVICES TAP In addition to the solenoid operated inlet a second tap without an interlock valve is provided This valve can be used to connect air vacuum or non hazardous gas supplies to the cabinet 6 4 HYDROGEN PEROXIDE CONNECTIONS On Peroxide versions of the cabinet connections designed to enable integration with a Clarus Hydrogen Peroxide Generator are fitted This is used to de contaminate the cabinet see section 7 6 5 FORMALIN VAPORISER A Formalin Vaporiser Fig 8 can be fitted to the cabinet to provide a convenient method for decontamination The vaporiser can function as part of the integrated fumigation cycle Refer to Section 7 BIOQUELL UK LTD TMOI 0 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 20 of 61 Web www bioquell com United Kingdom 7 i m m uu Figure 8 Formalin Vaporiser 6 06 FIREBOY GAS BURNER This burner has been designed for use in Safety Cabinets and Laminar Flow Units and can be supplied with a foot operated switch The switch operates a solenoid valve allowing the gas to flow and at the same time initiates its ignition The burner can either be set for a fixed time of operation or can be extinguished by pressing the foot switch a second time The advantage of this type of burner is that the flame is only present wh
49. he cabinet s main fan can be achieved by use of the Slider labelled 9a in Fig 13 Manual adjustments may be necessary when a specific flow is required in the cabinet but the use of this device is expected to be limited to occasions of testing and evaluation To use the manual speed adjustment the software should firstly be Connected then manual control can be enabled by clicking on Options followed by Direct Control Note that as a default this option is selected Whenever the controller and computer are Dis Connected the fan will initially operate at the speed that was previous set The manual speed control slider is a method of dictating the preliminary speed setting from which achievement of the set point will be affected Because controlling action is necessarily quite slow the alarms points may be checked by employing suitable starting points for the fan and then enabling controller action by clicking on Dis Connect 10 PC Clock This field shows the time and date of the PC s internal clock It is important that the PC s clock always reports the correct time and date as this is the reference used to re set the controller clock whenever a connection is made between the two devices the controller clock is automatically updated from the PC clock For security purposes the controller has been programmed so that it will not accept a time update grossly behind its current reference 10 5 MENU BAR Facilities available from the to
50. he cabinet and then exhausting it out through a HEPA filter The mean inflow must be between 0 7 and 1 0 m s to ensure operator protection is maintained If the airflow should fall outside these limits the cabinet will alarm The cabinet can be either a ducted version where all the air is exhausted out of the cabinet up a duct at the end of which the fan is located The cabinet can control the fan to maintain the correct airflow rate if supplied by BIOQUELL The alternative version re circulates the air back into the laboratory after passing through two exhaust filters note this configuration is not covered by EN12469 2000 This Cabinet is suitable for working with Category 1 2 and 3 pathogens The airflow patterns are shown below in figures land 2 along with the sample points i NU pnt BIOQUELL UK LTD 44 0 1264 835835 52 Royce Close 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Web www bioquell com United Kingdom 2 1 RESPONSIBILITY OF THE USER It is not possible to detail all the Safety requirements for every procedure undertaken inside a Safety Cabinet It is the responsibility of the user to ensure safe and proper techniques Contact BIOQUELL for advice if required 3 SPECIFICATION 3 1 OPERATING ENVIRONMENT The cabinet will operate satisfactorily in environments where the ambient temperature lies between 10 and 35 C Condensation of mois
51. icked or for the smallest adjustment either direction arrow may be clicked When the correct speed has been obtained as indicated on the anemometer although the software waits until sensor values are stable it is advisable to observe the sensor values until the numbers are steady before clicking Continue Click on the Continue button A new dialog box will appear Please wait for Stabilisation see Fig 15 Wait until this clears if this fails to clear within 10 minutes there is a problem with the flow sensor as it is not Stabilising seek technical assistant After the two alarm points have been set a dialogue box see Fig 16 will offer the choice either to Continue which will store the data to the controller s memory or to Cancel the calibration process If the latter is selected then flow settings and alarm values in the controller s memory will remain unchanged E CLASS 1 BSC Setup Program V 1 00 x Fille FIN nos Comm Pert Options Messages Languages SenalMa MICROFLOW a 24 03 00 14 10 Choose Continue to write data Es 24 03 00 14 03 J 24 03 01 5 3 months C B months C 9 months 5 12 months No Change Choose Cancel to discard iT 2459 Cancel fi a gt Figure 16 End of Calibration Dialog Box Continue Click on Continue and after a short period a new dialogue box will appear Calibration Complete Press OK Another screen will appear saying Data written to contro
52. ind the operator movement of other staff without disturbing the air flow patterns It is recommended that a smoke test be performed in the laboratory where a Cabinet is to be sited to ensure that there are no adverse movements of air A smoke test should also be conducted after the Cabinet has been installed to check the air movements and room pressure while this Cabinet is running 4 2 POSITION OF THE FAN FOR DUCTED CABINETS The Fan should be positioned at the exhaust end of the ductwork which should terminate outside thus ensuring that internal ductwork is kept under negative pressure Should it be necessary to install the Fan inside the building then the ductwork from the Fan to the outside of the building must BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 10 of 61 Web www bioquell com United Kingdom be kept as short as possible and never longer than two metres Ductwork should be constructed from a smooth non porous material e g rigid PVC The length of the ducting should be kept as short as possible the fan must be sized for the duct work and an additional pressure drop from the cabinet The following table gives the pressure drop and the flow volumes the duct work must be designed to Ducted Integral Min Normal Max Cabinet Bypass Airflow Airflow Airflow Pressure Pressure M hr M
53. ions Service and maintenance operations other than those covered by this manual should only be undertaken by BIOQUELL s recommended service agent see Section 16 Care should be taken to follow the instructions in this manual to ensure safe operation However if further clarification is required contact your supplier 1 1 SAFETY ENVIRONMENTAL CONDITIONS The cabinet is for indoor use only The unit is safe to run electrically between 5 C and 40 C at 10 to 8096 humidity however the unit ought not to be operated outside a temperature range of 10 to 35 C for it to give the designed operator protection Installation category overvoltage category II BS 7671 Microflow is a registered trademark of BIOQUELL plc Windows is a trademark of Microsoft Corporation BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 5 of 61 Web www bioquell com United Kingdom 2 INTRODUCTION AND PRINCIPLE OF OPERATION The Microflow Cabinet is the result of many years of experience in the development and use of Safety Cabinets and should give long and trouble free service provided these simple instructions are followed The Microflow ABS Class I Cabinet Mk Il has been designed to EN 12469 2000 which provides operator protection for handling organisms This is achieved by constantly taking air from the room into t
54. ir from the room To start the MSC the user should follow the procedure below e Enable the damper by pressing the fan button T e Damper will start to open e The cabinet will prompt the user to open the window after a few seconds with a message Open Window and the sounder will operate e Fan starts e Cabinet will stabilise this takes one minute e The cabinet will then display SAFE MODE NOTE If the User opens the window then the cabinet will Alarm and the following message will appear Window Open Fan not On BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 17 of 61 Web www bioquell com United Kingdom To switch off the Cabinet and extract air from the room EITHER recommended e Fan button can be pressed e Cabinet will alarm prompting the user to shut the window e Damper will close off cabinet OR e The window is shut e Damper will close off cabinet If the fan requires switching off and the window is required to remain open then this can be achieved without the Cabinet alarming with the following procedure e Fan Time delay button is pressed 9 e Cabinet continues to run for a pre set time e The damper will close off the cabinet 5 3 WORK SURFACE The actual working surface is lower than its edges so that minor spillages will be contained 5 4 C
55. le 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover i iri i Email enquiries bioquell com Hampshire SP10 3TS Page 9 of 61 Web www bioquell com United Kingdom 4 INSTALLATION WARNING INSTALLATION SHOULD ONLY BE CARRIED OUT BY TRAINED AND APPROVED ENGINEERS OR AGENTS BIOQUELL OR ITS AGENTS CANNOT ACCEPT RESPONSIBILITY FOR DAMAGE LOSS OR INJURY CAUSED BY OR RESULTING FROM INCORRECTLY INSTALLED EQUIPMENT WARNING DO NOT USE THE PEROXIDE BLOCK OR PIPES FOR LIFTING 4 1 POSITION OF THE CABINET The correct functioning of a Safety Cabinet can only be achieved if the Cabinet is properly installed The position of the Cabinet in the Laboratory is important Advice on the correct location of Cabinets is given in The Code of Practice for Prevention of Infections in Clinical Laboratories and Post Mortem Rooms published by HMSO and BS EN 12469 2000 and BS5726 2000 The following points should be observed in selecting the position for the Cabinet a Is the room of adequate size About 25 cubic meters 900 cubic feet is generally considered to be the minimum for ducted cabinets unless special provision is made for make up alr b Is the make up air adequate c Will the Cabinet be subject to cross movements of air generated either by open windows the ventilation system exhausts from re circulation cabinets or doors being opened and closed near the Cabinet d Is there space beh
56. ller press OK button Should the message appear Write BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 52 of 61 Web www bioquell com United Kingdom failed try again with two buttons Yes and No This message does not necessarily mean that it has actually failed Press NO Calibration is now complete break the communication link between the PC and controller by clicking Dis Connect button Note If new setup parameters different from configuration files already stored on the PC s disk are required to be saved for future use this operation should be carried out before Dis Connecting To save a configuration file edit all variables to the figures required on the screen then execute the file interface Finally click on Save For further details see Section 10 5 1 File BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 53 of 61 Web www bioquell com United Kingdom 12 TROUBLE SHOOTING Fan and light do not Check Mains Power Indicator is illuminated if operate not e Check power to unit e Check isolator switch adjacent to mains supply input socket is ON Inflow High or Low Check for obstructions on the exhaust outlet or Al
57. me O R Cumberlege Position Director Date 10 March 2010 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 57 of 61 Web www bioquell com United Kingdom 16 APPROVED SERVICE COMPANY We only recommend BIOQUELL in the UK or its local service provider to carry Out service and repair work These technicians have been fully trained on the product To contact BIOQUELL use one of the following methods e Call the Service Desk on 01264 835 800 or 0800 527800 e Faxto 01264 835 801 e E mail to enquiries bioquell com For details of our local service providers outside the UK contact BIOQUELL on 44 1264 835 800 BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 58 of 61 Web www bioquell com United Kingdom 17 SAFETY SYMBOLS BIOHAZARD CAUTION CAUTION RISK OF ELECTRIC SHOCK FAN EARTH GROUND TERMINAL PROTECTIVE CONDUCTOR TERMINAL POWER ON OFF ZY oO ORE BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 59 of 61 Web www bioquell com United Kingdom 18 WARRANTY BIOQUELL UK Ltd p
58. mum 1 A at 24 V a c or d c Refer to Fig 9 for connection details DESIGNATION CONNECTION ALARM F O OQ Le p common C Figure 9 Volt Free Contact Wiring 6 10 INTERNAL SOCKET RESIDUAL CURRENT DEVICE As an additional operator safety feature a Residual Current Device RCD is available and when fitted is located adjacent to the circuit breaker and main ON OFF switch on top of the cabinet see figure 6 The device will isolate power to the socket if a difference in currents flowing along live and neutral conductors exceeds 30 mA To set or reset the RCD push the black lever to the On position It is recommended that the RCD is checked for correct operation before each time it is re set To test press the TEST button on the RCD once the black lever has been set to On the black switch should then immediately go to the Off position 6 11 U V LIGHT The U V light is not recommended for use in Safety Cabinets in EN 12469 2000 and is an inappropriate method to de activate category 4 pathogens Any surface that is not in contact with the direct UV rays will not be disinfected This will include the rear and sides of the portable UV lamp assembly Therefore when the lamp is removed it must be cleaned using a proprietary disinfectant The UV light transmission from the tube reduces with use and thus the tube ought to be replaced regularly The UV light is operated on a timer of a default of 15 minutes If the exposure to th
59. on files is described in Section 10 5 1 File The fields labelled 6a and 6b refer to alarm values in m s of high inflow and low inflow respectively To create a configuration file click the pointer in the relevant field press the Delete key as required and enter the desired value When all other desired parameters have been entered select Save as described in Section 10 5 1 2 Save to store the settings on the PC disk 7 Sensor Output Providing the Show sensors option has been selected from the Options menu the fields adjacent to the symbols will display a numerical representation of the inflow sensor output values The numbers displayed between O and 255 proportionally relate although not linearly to the airflow over the respective sensor Thus observation of the values can be of assistance during calibration diagnosis of faults and commissioning Note As a default Show sensors is selected 8 Setpoint Adjustment BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 44 of 61 Web www bioquell com United Kingdom This slider allows the controlled operating point of the cabinet fan s speed to be adjusted once calibration has been performed 9 Fan Speed Adjustment This control allows the speed of the main fan to be adjusted 9a Main Fan Speed Manual speed control of t
60. ow gives the working area for the different cabinet sizes NEN A m mm mm mm 1 0m Cabinet 635 1000 530 1 2m Cabinet 1200 1 5m Cabinet 1500 The safe working area is shown on the label on the front of the unit and is all the internal working area 3 6 NON RETURN VALVES DUCTED A Non Return Valve is located after the HEPA exhaust HEPA filter s in the top box plenum This is to stop blow back and may not be 100 gas tight but there will be no leakage of vapour if operated at negative pressure when being de contaminated The top box may be turned so that the exhaust ductwork may be taken either from the right or left hand side 3 7 ALARMS Safety has been considered the primary factor in the design of this cabinet Consistent with this an alarm condition will be indicated when the following conditions occur c Ti Tr airflow more than calibrated alarm limit Inflow high gas solenoid airflow less than calibrated alarm limit Inflow low gas solenoid LH unlatched If front screen open and right hand side unlatched Warning screen Warning only RH unlatched Warning only If the speed of the exhaust fan required to maintain the inflow at the Warning calibrated setpoint is greater than 8096 of full speed replace filters Warning screen BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell
61. p menu refer to Fig 13 are described in the following paragraphs To display the possible options click with the pointer on the appropriate text The available options will then be displayed in black text Options not available will be shown in grey 10 5 1 File The Load and Save options allow sets of configurations to be stored on computer disc and retrieved for subsequent use This therefore will save time entering values each time a cabinet is set up or a series of cabinets are to be set to operate in the same way However since controller sensor values are not stored in the configuration file it is essential that each cabinet is calibrated using the facility described in Section 11 4 Calibration Procedure BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 45 of 61 Web www bioquell com United Kingdom 10 5 1 1 Load To load a configuration a Click on File followed by Load b All files with extensions cfg will be displayed select the appropriate by double clicking on it or type its name in the File Name box Click on OK or alternatively double click on the appropriate directory in the Directories or Drives boxes if it is stored C All the set up parameters will then be displayed on the screen 10 5 1 2 Save To save a configuration a Enter all the data in the relevant fiel
62. process will configure the controller 11 4 1 Initial Set up and measurement of inflow Configure the cabinet in Class I mode Place the head of the anemometer at the geometric centre of the front aperture in the vertical plane and in line with the edge of the glass wait for at least one minute for the reading to Stabilise and then record then place the anemometer at each of its four corners with the centre of the anemometer between 50mm and 55mm from the side and top or bottom The average of these five readings is the figure which ought to be used for the inflow The window should be secured in the working position If necessary place a draft exclusion screen in front of the unit under test Select Dis Connect to connect the unit under test Adjust the Fan Speed Slider until a reading of 0 85 m s 170 ft min is obtained then allow the unit to settle for five minutes Move the anemometer head to the four corners of the working aperture and record the reading along with the geometric centre three readings along the geometric centre on 1 5m units Establish the mean and place the anemometer head in the position closest to the mean Click on Calibrate area 11 Fig 13 The configuration file opening screen will then appear Select the default file default cfg double click on the appropriate text 11 4 2 Low Inflow A dialogue box will now appear see Fig 14 The value of velocity the instruction specifies to set will be the valu
63. reen locked password operation shut Remove blanking plate and open window xx Denotes a preconfigured time which counts down in minutes The defaults are Evaporation 30 minutes Fumigation 240 minutes Purging 480 minutes These defaults can be changed by use of the Set up software Caution The operation of the automatic fumigating system does not require ventilation to an exhaust system however IT IS NECESSARY TO PROVIDE VENTILATION FOR THE ROOM It is recommended at least two air changes per hour this will ensure that all traces of formaldehyde are removed from the room 7 3 2 2 Carbon Filter Changing The carbon filter will complete a maximum of ten fumigating cycles as indicated on the cycle counter at the beginning and end of every cycle after which it ought to be replaced The filter is replaced by slackening the four screws Clamping the filter in place then lifting the top half up and sliding out the filter To fit the new filter follow the procedure in reverse On fitting a new carbon filter the cycle counter will require resetting in the following manner a Press the key switch and enter the VAP code 2222 D Select 2 reset cycle counter to zero The removed carbon filter will be contaminated with formaldehyde Handling of the filter will require suitable personnel protection equipment consult your Health and Safety Officer Environmental regulation for the disposal of such material may be applica
64. roduces products that are warranted under normal usage against defects in workmanship and materials for one year parts and labour costs from the date of manufacture The Warranty is stated in the Standard Terms and Conditions of sale Export and Agent retailed products are warranted directly by the Agent Please confirm your warranty and liability status with the Agent In addition the Warranty is void unless the following conditions are met a The product has been installed and used as stated within the Instruction Manual D The warranty does NOT include servicing or maintenance An approved service company who have attended our training courses for your product must carry out maintenance of product Failure to maintain or service this product will invalidate the warranty Maintenance must be carried out in accordance with the Service Manual and include tasks within stated periods Failure to use approved service companies or BIOQUELL UK Ltd trained personnel for maintenance also affects the CE Marking status of the product removing BIOQUELL s Duty of Care and responsibility C Consumables such as pre filters HEPA filters light bulbs and tubes not warranted d This Warranty is void if faults are caused by accidental damage mishandling adjustment by unauthorised personnel or failure to follow the correct maintenance and safety precautions as stated in the Instruction Manual e The Warranty expressly provided for herein
65. rol system is that if the Window is disturbed during the evaporation or fumigating processes the fan will automatically be switched on in order to safely expel the fumigant up the duct work It is therefore most important the extract duct work is fitted prior to the start of the cycle In such a situation the fan will continue to run until the Keylock button is pressed and the fumigation PIN number has been entered for a second time The recommended quantities of the solutions used in the following procedures are SOLUTION 1 0 m 1 2 m 1 5 m CABINET CABINET CABINET Solution 40 w v ml De lonised Water ml 17 22 26 Ensure that the ducting to expel the Formalin is secure to the exhaust plenum and that any manual valves are open Add the formaldehyde solution to the deionised water This must be carried out in a fume cupboard or a Class I safety cabinet ducted outside Remove the cap of the vaporiser add the diluted formaldehyde solution and replace the cap Press the VAP switch and enter 2222 PIN number Close the cabinet window and lock the T handles shut The vaporiser operates automatically and takes approximately 30 minutes to evaporate the contents The sterilising cycle is set at 240 minutes After this time the cycle changes to Purging set for 480 minutes When the fumigation cycle is complete the display will show the following alternating messages Fumigation cycle Purging required and Please op
66. s Formalin sterilises inside Fumigating xx mins to run of the cabinet Fan circulates fumigant for Circulating one 5 second phases Fumigation completed Alternating messages Fumigation cycle Purging required and Please open Class I window Open Window Fan will start to purge Purging xx mins to run fumigant from cabinet When purge time is expired the cabinet will resume normal operation Xx Denotes a preconfigured time which counts down in minutes The defaults are Evaporation 30 minutes Fumigation 240 minutes Purging 480 minutes These defaults can be changed by use of the Set up software 7 3 2 Automatic Fumigation Option K The Option K units have a special activated Carbon Filter designed to remove Formalin vapor The cabinet also automatically progresses through the fumigation cycle Each stage is displayed with time to run in minutes 7 3 2 1 Running an Automatic Cycle Add the formaldehyde solution to the deionised water This must be carried out in a fume cupboard or a Class I safety cabinet ducted outside Refer to the table in section 7 2 1 for quantities Close the front window and tighten both handles and lock If re circulatory secure blanking plate over exhaust as described in 7 1 A notice should be attached to the cabinet to indicate that the fumigation cycle is operating THE CYCLE MUST NOT BE STOPPED The fumigation cycle will not commence unless the window is de
67. ssure Tube Connection There are two different types of cycles which are available either a filter decontamination cycle which ought to be used if the filters are going to be changed or the filter integrity is to be broken for any other reason Or there BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 25 of 61 Web www bioquell com United Kingdom is a workspace decontamination cycle which can be used to decontaminate the working area This is a suitable procedure when general servicing of the cabinet is to be done or a bio decontamination is required between experiments to stop cross contamination The advantage of the workspace only decontamination is that it is significantly shorter Running a Filter Decontamination Cycle a Run a decontamination cycle with the Clarus according to the Clarus manual The following cycle parameters ought to be set for bio decontamination of the filters Remote Start OFF Pressure Test Only OFF Test Pressure Test Time Test decay can be ignored Aeration Only OFF Pressure Control ON Aeration Pressure Control OFF Pressure Setpoint 10Pa Pressure Low Alarm 80Pa Pressure High Alarm OPa Airflow setpoint 20 m3 hr Delivery Temperature 60 C Conditioning Parametric OFF Conditioning Time 10 minutes Gassing Time 25 mins for 1 0 amp 1 2m and 35mins
68. t clean and free of unnecessary equipment Decomtamination would be necessary at the following times a Before carrying out any maintenance work b Before changing high efficiency filters c Before performance testing is carried out d Before moving relocating or in any way disturbing the cabinet e Before instituting a change in the work program f After a gross spillage as defined by your Safety Officer g To decontaminate any equipment or materials before their removal from the cabinet h Periodically as required by your Safety Officer 7 1 SEALING OF RE CIRCULATORY CABINETS PRIOR TO DECONTAMINATION When using Hydrogen Peroxide to decontaminate a cabinet the exhaust must be sealed The sealing plate should be placed onto the exhaust and clamped down using the 8 securing thumb nuts see figure 10 Once the decontamination cycle has been completed the plate must be removed prior to starting the cabinet Figure 10 Showing the fitting of the sealing plate BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 24 of 61 Web www bioquell com United Kingdom 7 2 DECONTAMINATION WITH HYDROGEN PEROXIDE This method of bio decontamination requires a BIOQUELL Clarus Hydrogen Peroxide Gas Generator alternatively the Bioquell EBDS Hydrogen Peroxide Bio decontamination service
69. tected as being locked Ensure that the cabinet is free of hydrochloric acid and other chemicals that may contain chlorines BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 31 of 61 Web www bioquell com United Kingdom Remove the cap of the vaporiser add the diluted formaldehyde solution and replace the cap Press the VAP switch and enter 2222 PIN number The cabinet will then display Fumigation Cycle No X X counts from 1 to 10 If run again after the 10th cycle the following message will be displayed Maximum cycles for filter reached and the unit will not run any further cycles until the filter has been changed and the counter reset see section 722 212 Evaporating X minutes to run will be displayed The vaporiser operates automatically and runs for the set time X to evaporate the contents A warning is displayed not to open the window The unit will then progress into fumigating with displaying the time to run and repeating the warning not to open the window Finally the purging stage starts with a repeat not to open the window on the display This is where the vapor will be automatically circulated through the filter where the formalin vapor is scrubbed out When the cabinet has finished the cycle it displays the following message Fumigation cycle No X complete
70. ture on the cabinet must be avoided at all times as this will affect filter efficiency 3 2 CONSTRUCTION The outer shell is of mild steel sheet finished in white powder coating The work area is made from 316 stainless steel with the sides and rear powder coated 3 3 DIMENSIONS WEIGHT ele cr cir CABINET CABINET CABINET including top box no top box Depth mm 748 748 748 Width mm 1190 1390 1690 Weight kg Ducted 180 210 230 Re circulatory 190 220 240 The maximum load of 25kg which can be placed on the cabinet floor with a foot print of 300mm by 300mm Large point loads must be avoided 3 4 ELECTRICAL Always disconnect the mains supply before removing any cover displaying the following label WARNING Disconnect the mains supply before removing this cover The ABS cabinet requires BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 i iri i Email enquiries bioquell com Hampshire SP10 3TS Page 7 of 61 Web www bioquell com United Kingdom a 230 V 10 50 Hz single phase supply Power W 1 0m 1 2m 1 5m CABINET CABINET CABINET 0 78kW 0 80kW 0 81kW Re Circulatory 0 78kW 0 80kW 0 81kW Includes standard external fan at end of duct run The internal control system operates at 5 V 3 5 WORKING AREA The working area is made of high grade 316L stainless steel The table bel
71. uiries bioquell com Hampshire SP10 3TS Page 46 of 61 Web www bioquell com United Kingdom 10 5 2 2 Reset Resets the two codes to default values The default values will be displayed but for reference they are Fan functions locking code 1111 Vapouriser function access code 2222 The configuration of different codes must be carried out from the controller keypad once communication between controller and computer has been broken To break the link click on the Dis connect button Note The current status of the communication link between the PC and the controller is indicated by the graphic on the Dis Connect button When the communication link is disconnected the two connectors are shown separated when connected they are together The status of the communication link should not be confused with physical condition of whether the cable is connected or not e g the cable may be connected but the communication link may not be made 10 5 3 Comm Port The Comm Port menu options allow the selection of either COM1 or COM2 serial ports on the computer to be used for communication to the controller By default COM2 will be selected but the assignment can be changed to COMI by clicking on the appropriate text If a portable computer is used for setting up the use of COMI is likely to be the only serial port available for external connections Thus it will be necessary to make this selection before connection to the controller can b
72. umigating with Formalin the cycle will re start automatically from the beginning of the phase it was in when the power disconnection occurred 5 5 3 Hour Meter and Service Date Due These parameters can be viewed on the display by pressing the TEST button BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 19 of 61 Web www bioquell com United Kingdom 6 OPTIONAL EXTRAS Figure 7 Services Double Electrical Sockets Gas tap and Formalin Vaporiser inlet 6 1 SOLENOID OPERATED GAS INLET Gases for use inside the cabinet are supplied via a solenoid valve located behind the side panel which permits gas to flow only when the cabinet fans are operating and airflow levels are within the specified parameters Switch on the gas supply by pressing the button marked GAS on the Control Panel The gas flow can then be controlled at the tap 6 2 MAINS SOCKET A double mains socket IP55 can be fitted to the side wall of the cabinet Most cabinets are fitted with two similar electrical sockets Equipment in excess of 300 W should not be connected to a socket if this value is exceeded the circuit breaker will trip The circuit breaker is located on the top right of the cabinet adjacent to the mains connection socket and on off switch see figure 6 Electrical safety test on the electrical socket
73. unication is established between the computer and controller Ensure that the computer date time is correct adjusting it if necessary using the date time function To update the clock click on Connect If all other operations are completed break the computer controller link by clicking on Dis Connect BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover i iri i l Email enquiries bioquell com Hampshire SP10 3TS Page 42 of 61 Web www bioquell com United Kingdom 3 Timer Settings The settings for the timers in 3a to 3c are changed by clicking in the appropriate box and then typing the required time The time entered is in minutes for which the device is active Values can range between 0 and 255 minutes for the mute timer and 0 to 999 minutes for other parameters For further descriptions of the functions referenced refer to Section 5 1 Control Panel 3a Overrun timer Pressing the FAN DELAY OFF button causes the fan to continue running for the time period entered 3b UV timer Pressing the UV button switches the UV lamp on for the time period entered 3c Mute timer Pressing the MUTE button mutes the buzzer for the time period entered 4 Vaporiser Cycle The vaporisation cycle consists of sequential phases with configurable durations of between 0 and 999 minutes To change the settings after Connecting click in the relevant bo
74. utions to any stainless steel metalwork as this will potentially Cause staining BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 38 of 61 Web www bioquell com United Kingdom Do not apply heat abrasive materials solvents or solvent wipes to the Surface as this can cause irreparable damage 9 4 PAINTED AND OTHER COMPONENTS Caution Do not use water around electrical connections switches etc To clean all painted and other components use a damp cloth and when required a liquid detergent Note Do not use abrasive cleaners Do not use Chlorine based cleaning agents on painted surfaces BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close ci Fax 44 0 1264 835836 West Portway Andover Revision 3 Email enquiries bioquell com Hampshire SP10 3TS Page 39 of 61 Web www bioquell com United Kingdom 10 CONTROL SYSTEM CUSTOMISATION AND CALIBRATION It is strongly recommended that BIOQUELL or a trained agent of BIOQUELL is used to customise and calibrate the cabinet To do this work a PC with appropriate operating system is required as well as the set up software which is available from BIOQUELL 10 1 INTRODUCTION The microprocessor based control system used in the cabinet allows the user two main advantages over the conventional analogue system These are
75. with the airflow control system to operate Alarms are muted for a period of about 1 minute during which time the display will show the message STABILISING FLOW If after this time no alarms are active the correct airflow will have been established and the display will show an appropriate message for example CABINET SAFE A second press of the fan button causes the fan s to be switched off Alternatively one of the other fan controls or the vaporisation cycle can be selected to provide the desired action 5 1 4 Fan Off Delay Pressing this button when the fan has been running LEDs of buttons with numbers 1 or 3 lit will cause the fan to be switched off after expiry of a timed period The delay period is configurable from the set up software See Section 10 Control System Customisation and Calibration Pressing this button a second time cancels the remaining delay time and switches off the fan s BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 13 of 61 Web www bioquell com United Kingdom Once the fan delay is activated it can alternatively be cancelled by pressing either of the other two fan functions buttons 1 or 3 This will initiate the relevant function 5 1 5 Fan 100 Pressing this button will cause the cabinet s internal or external o
76. x and edit as required If these are the only operations to be carried out after amending the values click on Write followed by Disconnect 4a On time This is the time at the start of the cycle when the vaporiser heater is powered Choose a suitable time to achieve evaporation of all of the fumigant solution but not to run the heater needlessly A thermostat will break and reset power to the unit if the evaporation temperature is exceeded As a guide if 22 ml of approximately 40 solution with an equal volume of water is being used then 26 minutes should be an appropriate value for this timer 4b Fumigation time This is the time required for the fumigant to sterilise the cabinet internal Surfaces and all installed components Thus choice of an appropriate value will depend on the sterilising agent its concentration and the nature of the source of contamination within the cabinet A figure of 240 minutes is suggested as a suitable value assuming 40 solution Formaldehyde is being used 4c Purge time This is the time period following fumigation when the fan operates to extract the sterilising agent out of the cabinet Again careful consideration needs to be given to the choice of this value since an adequate time needs to be BIOQUELL UK LTD TMO10 O amp M 001 Tele 44 0 1264 835835 52 Royce Close Revision 3 Fax 44 0 1264 835836 West Portway Andover Email enquiries bioquell com Hampshire SP10 3TS Page 43 of 61

USER MANUAL - Astec Microflow

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