Sonifier® Cell Disruptor Model SLPe User's Manual
Contents
1. seessssseeeeneneee nnns 4 8 installation and setup sseeseseseesenereeeenne nnns 4 1 installation checklist essen 4 1 tendedero 1 2 Interconnect Diagram sss 7 6 Introductions ete Sean ait fey tli eode 2 1 IE 2 4 A O 6 2 index ii 100 214 254 Rev A SLPe Cell Disruptor User Manual M MAINTENANCE sees eset titre beet ree hee ets ect Shee wa ha ham abetted 7 1 Membrane CHU kw akana 6 2 Membrane Keypad swwsswwmanumasimawaniai niaminini nnns 6 1 MACHO WA AA E WA cou etch ete Ma AA t dte 6 9 MOJOS altiro AAA NE BINAGI et eee secos tbid esee eed aid bo ee obi e te Dto e di 6 4 N EIE to MM 6 4 O Operation eere a eet es 6 1 out of range PAaraMetelS cecceceeeeeeseeeeeeeeeeesaeeeeeeeeetsaeeteaes 6 6 6 7 OVOIVIOW A da adi MANA ue dc dae edn 2 1 P physical description UA eene 5 2 power output loss sssssssssesseneeeeenenennm enne nens 7 2 power requirements Tete EDDIE 4 8 POWOLSWIICI eedem dede noti nt tee ecd 6 1 precautions sco debi a dae qa e Eee WA 1 2 Pulse Mode ici ett rr ecce sheen ates 2 2 Pulse Pause MOJA azua 2 2 R Rosal IA WI MA ee o tees 4 4 100 214 254 Rev A index iii BRANSON S CC HR K kaiee aaa 1 1 AUS nnns 1 1 Safety equipimelt d oat Lee eet tet eed 4 9 safety Measures wamuumiza wizi 1 2 setting up the workplace wwwwwwwwwwwamma2. 1 4 14 Warranty 1 5 15 Howto Contact Branson 1 6 1 5 1 Before Calling Branson for Assistance 1 6 Chapter 2 Introduction to the SLPe Cell Disruptor 2 1 Overview of the SLPe 2 1 2 2 Controls and Commands 2 2 2 2 1 SLPe Cell Disruptor Keypad Display Description 2 4 2 2 2 Back Panel Connections 2 5 2 3 System Features 2 6 Chapter 3 Delivery and Handling 3 14 Delivery and Handling 3 1 Chapter 4 Installation and Setup 41 Installation Checklist 4 1 4 2 System Block Diagram 4 2 4 3 System Component Description 4 2 4 3 1 Standard Components 4 2 4 3 2 Tools 4 3 4 3 3 Accessories 25 Sse Se Se ee ee 4 4 44 Assembling the Equipment 4 6 4 4 1 Setup Procedure 4 6 100 214 254 Rev A toc v BRANSON 4 4 2 Connecting Microtips and Converters 2r 45 Input Power Requirements 4 8 46 Electrical Connections to Equipment 4 8 4 6 1 POWCRCOM AA EA 4 8 4 6 2 User I O Connection 4 9 4 7 Guards and
3. Chapter 7 100 214 254 Rev A 5 le ag IHNVd HVY3H OL GND JYV SNNOO TISHS A TIY t EEN 3lv d ONNO O OL 8 NOLO3NNOO QNnOHO SILYIIONI TOHWAS E Ni no wasi A ASN LON 34V NMOHS LON SNid Ty Z E OD AZ E KINO 13S QN VOBZ SV 30V1d38 y Tavis o qu Ave 5 S310N tt ume Lor
4. SLPe Cell Disruptor User Manual Table 2 1 System Trouble Analysis Chart Chapter 7 Maintenance Troubleshooting Charts Symptom Probable Cause Corrective Action Ultrasonic power is absent or inconsistent Tool is loose or has failed Tighten or replace defective tool Converter cable connection is loose or has failed Tighten converter cable on back of Sonifier Converter has failed Replace defective Con verter return for repair Power supply or controls have failed Return for repair Sonifier operates continu ously but does not oper ate in Pulsed mode Digital controls have failed Return for repair Slight electric shock when touching a metal part of the system or lab equip ment contacting the sys tem User I O signals are not working correctly System is not properly grounded Cordset has failed or had Ground lead removed User I O is not configured correctly e customer provided User I O components have failed or are no longer functioning e Outputs of User I O failed Correct electrical ground to system replace cordset Verify and correct con nections See Section Table 4 2 User I O Pin Out DB9F provided for customer designed interface beginning on page 6 9 Return unit for repair 100 214 254 Rev A 7 5 BRANSON Maintenance SLPe Interconnect Diagram 7 4 SLPe Interconnect Diagram
5. 3 Troubleshooting Charts 7 3 7 4 SLPe Interconnect Diagram 7 6 toc vi 100 214 254 Rev A SLPe Cell Disruptor Chapter 1 Safety and Support User Manual Safety Considerations Chapter 1 Safety and Support 11 Safety Considerations 1 1 2 General Precautions 2 1 2 1 Intended Use of the System 2 1 2 2 Safety Measures and Guards 2 1 2 3 Safe Operation 3 1 2 4 Setting Up the Workplace 3 1 3 Regulatory Compliance 3 14 Warranty 5 15 Howto Contact Branson 6 1 5 1 Before Calling Branson for Assistance 6 1 1 Safety Considerations Observe the following safety considerations when operating the Sonifier SLPe Cell Disrup tor Make sure that the equipment is properly grounded DO NOT operate if it is not The unit is equipped with a three conductor cord and three prong grounding type plug and must be plugged into a three prong grounding type wall receptacle DO NOT under any circumstances remove the power cord ground prong DO NOT operate the equipment with the cover removed High voltage is present within the equipment when connected to plant wiring DO NOT turn on the ultrasonics without the converter and tool attached DO NOT touch th
6. Chapter 7 4 Know your equipment model and serial numbers found on a data label on the units Information about the tool part number gain etc may be etched into the tooling Software or firmware based systems may provide a software version number which may be required The SLPe Cell Disruptor provides the firmware information initially on the start up screen immediately after the LED check 5 What tool and accessories are being used 6 What are the setup parameters and mode 7 Is your equipment in a remotely operated system If so what supplies the start signal 8 Describe the problem provide as much detail as possible For example is the problem intermittent How often does it occur How long before it occurs if you are just powering up If an error is occurring which error or message 9 List the steps you have already taken 10 What is your application including the materials being processed 11 Have a list of service or spare parts you have on hand tools accessories etc Notes 1 6 100 214 254 Rev A SLPe Cell Disruptor Chapter 1 Safety and Support User Manual How to Contact Branson Returning Equipment for Repair Before sending equipment for repair provide as much information with the equipment to help determine the problem with the system Fill in any details below or on a separate sheet 1 Describe the problem provide as much detail as possible For example is this a new problem Is the proble
7. Clear the condition that caused the alarm in order to be able to reset the power supply When you get an Overload Alarm in Experiment Mode the process has already overloaded 3 times You must press RESET This applies even if the configuration has been set to not require a reset for alarms 100 214 254 Rev A 6 9 Chapter 6 Operation BRANSON Alarms 6 10 100 214 254 Rev A SLPe Cell Disruptor Chapter 7 Maintenance User Manual Maintenance and Troubleshooting Chapter 7 Maintenance 7 1 Maintenance and Troubleshooting 1 7 2 Reconditioning the Converter Tool Interface 2 7 2 1 Refacing the Mating Surfaces 3 7 3 Troubleshooting Charts 4 74 SLPe Interconnect Diagram 7 7 1 Maintenance and Troubleshooting The SLPe Sonifier is a self contained system that requires no internal servicing except for a protective fuse and there are no user serviceable parts inside the unit The ultrasonic tooling may require periodic inspection and maintenance to ensure optimum performance The tooling components are subject to wear and may require replacement after a period of time depending on the applications If you have a problem operating your unit refer to Table 2 1 System Trouble Analysis Chart on page 4 to locate the symptom that most clearly describes your problem Tip Erosion Tools and microtips can e
8. Description 10 40 70 109 122 1064 0 250 Diameter Tapered 5 15 30 109 122 1182 0 190 Diameter Tapered 12 38 74 109 122 1065 0 125 Diameter Tapered 12 34 68 109 122 1066 0 091 Diameter Tapered 14 32 64 All measurements in microns unless otherwise specified 4 10 100 214 254 Rev A SLPe Cell Disruptor Chapter 5 Technical Specifications User Manual Technical Specifications Chapter 5 Technical Specifications 5 1 Technical Specifications 5 1 52 Physical Description 5 2 5 3 System Performance Benchmark 5 3 5 1 Technical Specifications The following table outlines the technical specifications for the Digital Sonifier 4 digit numeric display with provisions to set mode Continuous Time Time Pulse Energy Energy Pulse operating parame Controls and Displays Froni ters Amplitude Time On Time Pulse Joules and perform an rane executable Reset Test Enter Start Stop on front panel mem brane On Off rocker switch to turn on power to the unit Duty Cycle Intermittent pulse duration adjustable 0 1 seconds 99hrs 59 mins or continuous processing time Horn Frequency 40 kHz 93 to 182 VAC 50 60 Hz North American and Japan line Voltage 182 to 264 VAC 50 60 Hz CE and non CE units available Max Output Power 150 Watts Temperature Operating 41 F 5 C to 104 F 40 C ambient Stor
9. LED s 3A B C D E Reset Key When an alarm occurs during the process cycle and the Reset LED is blinking pressing the Reset key will clear the alarm If the LED is not on and RDY is displayed pressing the Reset key will send a reset to the power supply module When in the parameter entry mode pressing the Reset will restore the original value of the parameter being modified and then return the system to Ready It will also reset the power supply and clear any alarms Test Key The Test key is only active when the system is ready Ultrasonics are enabled while the Test key is pressed and disabled when released Test performs the run seek and store functions causing the power module to test for the resonant frequency of the converter and tool at 10 amplitude and then go to the amplitude set ting Enter Key Press the Enter key on the SLPe Cell Disruptor to Confirm a selection setting Switch between power display and experiment time remaining e Exit the configuration menu If you have entered a modified parameter value the system checks the value for validity and format If the value you entered is valid the value is accepted and it replaces the old value The original value is lost and cannot be recovered If the modified value is invalid an appropriate error message is displayed Start Stop Key The Start Stop key is used to set up Pulse Start in the configuration menu at start up Either Press and hold to run
10. Safety Equipment 4 9 4 8 Ultrasonic Test 4 10 Chapter 5 Technical Specifications 5 1 Technical Specifications 5 1 52 Physical Description 5 2 5 3 System Performance Benchmark 5 3 Chapter 6 Operation 6 1 Front Panel Controls 6 1 6 1 1 Power Switch 6 1 6 1 2 Membrane Keypad 6 1 6 2 Rear Inputs and Connections 6 4 6 3 System Modes 6 4 64 Menu Navigation 6 4 6 4 1 Configuration Menu 6 4 6 4 2 Control Menys e eoe 2d a eet ee ace dede mede i 6 5 6 5 Operational Sequence 6 7 6 5 1 SLPS in Continuous Mode 6 7 6 5 2 SLPe in Time Mode Either Low min sec or High hr min 6 8 6 5 3 SLPein Energy Mode 6 8 6 5 4 SLPe in Time Mode with Pulse Either Low min sec or High hr min 6 8 6 5 5 SLPe in Energy Mode with Pulse 6 8 6 6 Ai c viet ehh roy ee AAA AO AAA A 6 9 Chapter 7 Maintenance 7 1 Maintenance and Troubleshooting 7 1 7 2 Reconditioning the Converter Tool Interface 7 2 7 2 1 Refacing the Mating Surfaces 7 3 7
11. The unit should be positioned away from radiators and heating vents A fan inside the unit maintains a safe operating temperature in the power supply by circulating air over the components Therefore place the unit so that the air intake on the bottom of the power supply is not blocked Periodically unplug the unit and clean the air intake underneath the power supply to ensure that dust or dirt is not restricting the flow of air If the SLPe Cell Disruptor is to be used for remote operation ensure that the unit is situated within full view of the operator to prevent injury or equipment damage through an accidental or automatic start up 100 214 254 Rev A 1 3 Chapter 1 Safety and Support Regulatory Compliance BRANSON 1 3 Regulatory Compliance The SLPe Cell Disruptor is designed for compliance with the following regulatory guidelines Code of Federal Regulations Title 29 Part 1910 UL61010 A 1 CSA22 2 1010 1 European norms EN 61010 1 EN55011 EN61326 A1 EN61000 3 2 EN61000 3 3 EN61000 4 3 EN61000 4 5 EN61000 4 6 EN61000 4 11 IEC61000 4 2 A1 IEC61000 4 4 Following the provision of Low Voltage Directive 73 23 EEC amended by directive 93 68 EEC EMC Directive 89 336 EEC amended by directive 91 263 EEC 92 31 EEC and 93 68EEC FCC part 18 EC 529 IP 65 water resistant for Membrane Keypad The SLPe Cell Disruptor is CE compliant 1 4 100 214 254 Rev A SLPe Cell Disruptor Chapter 1 Safety and Support User Manua
12. User s Manual Forward Congratulations on your choice of a Branson Ultrasonics Corporation system The Branson SLPe Ultrasonic Power Supply is the newest generation of product using this sophisticated technology for a variety of customer applications This manual should be kept with the system Thank you for choosing a Branson product Introduction to this manual This manual is arranged into seven structured chapters which will help you find the infor mation you may need to know to safely handle install set up program operate and or maintain this product Additional information is provided in several Appendix chapters In the event you require additional assistance or information please contact our Product Support department see How to Contact Branson on page 6 for information on how to contact us or your local Branson representative BRANSON For Your Notes 100 214 253 Rev A SLPe Cell Disruptor User Manual Table of Contents Table of Contents toc v Chapter 1 Safety and Support 11 Safety Considerations 1 4 1 2 General Precautions 1 2 1 2 1 Intended Use of the System 1 2 1 2 2 Safety Measures and Guards 1 2 1 2 3 Safe Operation 1 3 1 2 4 Setting Up the Workplace 1 3 1 3 Regulatory Compliance
13. continuous operation and utilizing the trigger switch or in a time or energy mode using the SLPe controller With time or energy modes the trigger switch initiates the cycle which is then controlled by the SLPe 4 4 100 214 254 Rev A SLPt Cell Disruptor Chapter 4 Installation and Setup User Manual System Component Description Soundproof Enclosure Although ultrasound is above the normal range of human hearing audible sound sometimes occurs when liquids are treated ultrasonically especially due to cavitation produced by ultrasonic vibration The Sound proof Enclosure can be used to reduce this to an acceptable level It is especially useful when the Sonifier is used for extended periods of time The Soundproof Enclosure is also useful at minimizing splashing while the experiment is running Cooling within the enclosure may be required for certain applications Detail of the enclosure may vary from the Fig ure below This shows the tool about to be inserted into the adapter which securely positions the microtip when it is inserted into the opening in the top of the enclosure A ring stand support is located inside the chamber Figure 4 2 Soundproof Enclosure 100 214 254 Rev A 4 5 Chapter 4 Installation and Setup BRANSON Assembling the Equipment 4 4 Assembling the Equipment The SLPe Cell Disruptor unit is pre assembled and requires no special tools however other components must be connected to the unit in order for the
14. left The digit will blink to indicate it is ready to modify When in Configuration Mode it selects functions 9 Up Arrow Increments the selected digit one count per key press at an increment appropriate for the parameter selected When in the Configuration Menu this key toggles the function between Off 0 and On 1 100 214 254 Rev A 6 3 Chapter 6 Operation BRANSON Rear Inputs and Connections 6 2 Rear Inputs and Connections 1 Power input receptacle with fuse holder 2 RF output cable connector J1 3 User Input connector J2 Refer to Chapter 2 Figure 2 3 for a detailed rear view drawing Figure 6 2 User Input J2 Pin Connection Key IN OUT Feature Pins Feature Pins Reset 1 7 Alarm 4 7 Start 2 6 Ready 5 7 Seek 8 9 Cycle On 3 7 6 3 System Modes You can control the way in which ultrasonics are applied to your sample by setting the unit to operate in a particular mode You determine the mode in which to operate by specifying parameters for your process There are three standard system modes and one optional mode as described below Continuous Mode Ultrasonics are applied to the sample until you Stop the cycle Timed Mode Ultrasonics are applied to the sample for a period of time that you specify by setting the Time gt On parameter Pulse Mode Set pulse length of Time or Energy for Experiment parameter In Pulse Experiment Time mode ultrasonics can be turned on and off m
15. normal range of human hearing some applications can create audible noise above 85dB If an uncomfortable level of noise is present the operator should wear ear protection for safe operation Appropriate eye protection should be worn when operating the SLPe to prevent possible splash injury orig inating in the solution The Ultrasonic Horn can cause injury and or equipment damage during operation To avoid injury or accident never touch the Ultrasonic Horn while the System is turned on and do not allow the Horn to come in contact with solid vessels or supports The User I O may be used to remotely control the System If this is the case you must design in whatever safety precautions are appropriate to your User I O circuit design to prevent unexpected start up which can cause personal injury and can cause equipment damage 100 214 254 Rev A 4 9 Chapter 4 Installation and Setup Ultrasonic Test 4 8 Ultrasonic Test BRANSON The Test button on the front panel of the SLPe Cell Disruptor is used to verify that the unit is functioning providing ultrasonic energy to the Converter and Tool Later you can run another test on the system for your particular experiment described in 5 3 System Performance Benchmark Before testing the Sonifier always make sure that the tool is not touching anything The System also per forms several self tests when it is first turned on Step Do this To obtain this result oe Up
16. off operation When turned on the front panel membrane LED s will light during the power up sequence and the unit s fan will run 6 1 2 Membrane Keypad The membrane keypad on the front panel of the SLPe Sonifier allows you to enter parameters for both Sys tem Setup and Operation of the unit 100 214 254 Rev A 6 1 Chapter 6 Operation BRANSON Front Panel Controls Figure 6 1 SLPe Cell disruptor Front Panel Display Membrane a 9 Eg Y UM o Time hr min Covers Pot e HN EI ExpTime hr min __ 30 08 e Start Stop Energy imm NY 2050 Do not use a sharp or pointed object to press the front panel controls The soft touch membrane front panel can be permanently damaged O Digital LED Display Displays parameters All parameters to be set are viewed on this display During a weld cycle Peak Power is displayed and the lower 1A and upper half 1B of a colon will blink respectively to indicate sonics on and off While in Test mode the lower half 1A of the colon will be blinking Mode Selects and advances Mode selection 6 2 100 214 254 Rev A SLPe Cell Disruptor Chapter 6 Operation User Manual Front Panel Controls 2A Continuous Mode 2B Time Mode minutes seconds 2C Time Mode hours minutes 2D Energy Mod 2E Pulse Mode 8 Set Sets parameter values for selected Mode Time On LOW from 1 second to 99minutes 59 seconds Time On HIGH from 1 minute to 99 h
17. on the mating metal surfaces Corrosion can reduce or alter system performance Examine the mating sur face tool to converter and wipe the surfaces clean with a clean cloth or paper towel The SLPe Sonifiers do not require tuning Tuning is done in the factory and cannot be performed by the operator 7 2 Reconditioning the Converter Tool Interface Ultrasonic system components work most efficiently when the mating surfaces of the Converter and tool combination are flat in solid contact and free from fretting corrosion Poor contact between mating surfaces wastes power output makes tuning difficult increases noise and heat and may cause damage to the con verter 7 2 100 214 254 Rev A SLPe Cell Disruptor Chapter 7 Maintenance User Manual Troubleshooting Charts 7 2 1 Refacing the Mating Surfaces NOTICE Never clean the Converter or Tool mating surfaces with a buffing wheel 1 Disassemble the Converter Tool and wipe the mating surfaces with a clean cloth or paper towel 2 Examine all mating surfaces If any mating surface shows corrosion or a hard dark deposit recon dition it 3 Tape a clean sheet of 400 or finer grit emery cloth to a clean smooth flat surface such as a sheet of plate glass NOTICE Be careful to avoid tilting the part and losing flatness of the surface Doing so may make the system inoperative due to improper mating surfaces Lapping Procedure 4 Holding the part to recondition place th
18. send you a facsimile of the Returned Goods Authorization form to fill out and return with your equipment Branson Repair Department 41 Eagle Road Danbury Connecticut 06810 U S A direct telephone number 203 796 0575 fax number 203 796 0574 Provide as much information as possible that will help identify the need for repair Include a copy of Page 1 6 with your information filled in Carefully pack the equipment in original packing cartons Clearly label all shipping cartons with the RGA number on the outside of cartons as well as on your pack ing slip along with the reason for return Return general repairs by any convenient method Send priority repairs by air freight You must prepay the transportation charges FOB Danbury Connecticut U S A Obtaining Replacement Parts You can reach Branson Parts Store at the following telephone numbers Branson Part Store direct telephone number 203 796 0576 fax number 203 796 0574 Many parts can be shipped the same day if ordered before 2 30 p m Eastern time A parts list is in Chapter 4 of this manual listing descriptions and EDP part numbers If you need replace ment parts coordinate the following with your purchasing agent Purchase order number Ship to information Bill to information Shipping instructions air freight truck etc Any special instructions for example Hold at the airport and call Be sure to give a name and phone number Co
19. system to operate Some assembly of the ultrasonic horn is required as described in the following sections 4 4 1 Setup Procedure To set up your SLPe Cell Disruptor for an experiment take the following steps Step Action 1 Connect the microtip to the converter following the procedure in Section 4 4 2 Connecting Microtips and Converters on page 4 7 2 Mount the converter microtip assembly in a laboratory stand or other suit able support Secure the clamp on the converter housing 3 Set the ON OFF switch on the front of the unit to the O OFF position Plug the line cord into the unit and then into an appropriate electrical 4 power outlet ensuring that the power supply is grounded to prevent elec trical shock To remove a microtip use wrenches shipped with the system Never attempt to remove a microtip by hold ing the converter housing in a vise If necessary secure the largest portion of the microtip in a soft jawed vise See Section 4 4 2 Connecting Microtips and Converters on page 4 7 4 6 100 214 254 Rev A SLPt Cell Disruptor Chapter 4 Installation and Setup User Manual Assembling the Equipment 4 4 2 Connecting Microtips and Converters Connecting the Microtip to the Converter To connect the microtip to the converter take the following steps Step Action 1 Clean the contacting surfaces of the converter and microtip and remove any foreign matter from the threaded stud and thread
20. 1066 P N 109 122 1065 P N 109 122 1182 P N 109 122 1064 DO NOT allow the horn or microtip to contact lab stands beakers test tubes or similar objects Microtip failure may result Breakage of glassware may result in the loss of a speci men 100 214 254 Rev A 4 3 Chapter 4 Installation and Setup BRANSON System Component Description The tapered microtip attaches directly to the converter The tapered tip is recommended for applications such as spores fungi yeast muscle and connective tissue Excellent results can be obtained on volumes ranging from 3 to 20 ml in a relatively short period of time As the diameter of the tip decreases the ampli tude increases often requiring lower power settings for small volumes To prevent foaming or aerosoling while processing small quantities with the tapered microtips the use of a conical shaped tube such as a reaction vial or a cut down centrifuge tube is recommended When using microtips do not exceed a maximum Amplitude Control setting of 7096 The microtip will break if driven at higher amplitudes The bottom of the test tubes should not be in contact with the surface of the ultrasonic tool Such contact could cause breakage and loss of sample 4 3 3 Accessories Trigger Actuated Converter HT 415 P N 101 136 011 The HT 415 is a compact lightweight converter with the trigger switch located on the side of the barrel It may be operated in an untimed mode with the SLPe set for
21. 5s ae SOINOWSWH u z nal 3 s kn B ADEZ n 7 6 SLPe Cell Disruptor User Manual Index A assembling the equipment sesssseseeeeeennn back panel connections Benchmarks iaa an ba tuta Gd EE re f CET FRA SEEN benchmarki cte e A tete actu du aa eae loca Mi e tt es Branson NOW t CONTAGE iis icici ts nien ac aia compliance with RFI guidelines eseeenee component description w ww wwwmawwmamwma muwa www nano wanunua cnn components standard Pa AA ed connecting tips horns and converters ssssssesese connections back PANA oM E Continuous Mode see ene nnne continuous flow attachMeNlt ccoccnnicccccocccnnonocnnnnnononononononononnnnonananos A converters CONNECTING RM description PMY SICA AEE AAE a Digital Sonifier FPO CUICTIOMN n 100 214 254 Rev A index i BRANSON E electrical connections to equipment sssssseees 4 8 equipment assembling o dete ee e eb ite aaa 4 6 error Deep iioi eg eR itera bata a Bor ud 2 2 6 6 Experiment io tern ht it a Be ect erides 5 4 F IU RENE 2 6 Front Panel Controls ooooooccconccccnoncconocccnnnnncnnnnrn canon cnc cana entre 6 1 A O RAT 4 8 G guards and safety equipment s sse 4 9 H horns Connecting uie og tabe t eut 4 7 how to contact Branson sssseseee eene nenne 1 6 input power requirements
22. A SLPe Cell Disruptor Chapter 2 Introduction to the SLPe Cell Disruptor User Manual Controls and Commands Figure 2 2 SLPe Sonifier Cell Disruptor front panel Controls 2 Continuous Time min sec Time hr min IX Autotune Label Set m Cove rs Pot Amplitude ExpTime hr min __ S Start Stop Reset tes Pene Some functions of the SLPe Cell Disruptor can be controlled through the external input connector located on the rear of the unit You always have the ability to halt operation whether control is direct or remote Section 2 2 2 Back Panel Connections on page 2 5 describes the back panel of the unit 100 214 254 Rev A 2 3 Chapter 2 Introduction to the SLPe Cell Disruptor Controls and Commands BRANSON 2 2 1 SLPe Cell Disruptor Keypad Display Description LED Display A 4 digit LED numeric display is located on the front panel It is controlled by the Left and Up arrow keys located adjacently on the membrane keypad Arrows are used to respectively select and increment the selected digit This allows setup and navigation for controlling the SLPe Mode Select Key Each of the 5 SLPe Modes Continuous Time min sec Time hr min Energy Pulse can be selected by pressing the Mode key Repeat the press to advance to the desired operational mode The selected Mode LED will light Set Key Use this key to select the parameter s you want to enter update on the display
23. Diagram 4 2 System Block Diagram The block diagram shows the relative interaction of the components of a SLPe Cell Disruptor system E Eu eun einn EE ur eei rel i a Filter Harmonics 500W PFC Module only CE Units only Eg 3T seed i a Ji RF Out Step Down Transformer Power Supply Controller Control Board Board J2 External User I O 4 3 System Component Description 4 3 1 Standard Components The standard system components are SLPe Cell Disruptor unit AC Power cord Ultrasonic converter Tool Wrench set Manual 100 214 254 Rev A SLPt Cell Disruptor Chapter 4 Installation and Setup User Manual System Component Description Table 4 1 System Part Numbers Item Part Number Converter 4C 101 135 126 RF Cable N A attached Tool Kit 101 063 176 4 3 2 Tools Three sizes of microtips are available for use with Sonifier SLPe units While other tools may be available for use with the SLPe Cell Disruptor the primary tool is a microtip Microtips are useful for processing smaller volumes particularly where higher amplitudes are required Three sizes are available for use with SLPe units to meet specific application needs Figure 4 1 Tapered Microtip requires another spanner wrench P N 201 118 024 in addition to the one supplied in the tool kit 3 32 Microtip 1 8 Microtip 3 16 Microtip 1 4 Microtip P N 109 122
24. LPe Setup Form Make a copy of this form and use it to record a benchmark for your system s setup Date Operator Experiment Solution Digital Sonifier Model SLPe 120 Volt 240 Volt Unit Serial Number Converter Serial Number Tool Type Parameters Modes Amplitude setting LED read out Power Reading Other Setup Notes 5 4 100 214 254 Rev A SLPe Cell Disruptor Chapter 6 Operation User Manual Front Panel Controls Chapter 6 Operation 61 Front Panel Controls 2 6 1 1 Power Switch 2 6 1 2 Membrane Keypad 2 62 Rear Inputs and Connections 4 6 3 System Modes 4 64 Menu Navigation 5 6 4 1 Configuration Menu 5 6 4 2 Control Menu 6 65 Operational Sequence 7 6 5 1 SLPe in Continuous Mode 7 6 5 2 SLPe in Time Mode Either Low min sec or High hr min 8 6 5 3 SLPe in Energy Mode 8 6 5 4 SLPe in Time Mode with Pulse Either Low min sec or High hr min 8 6 5 5 SLPe in Energy Mode with Pulse 8 6 6 Alarms E e Spe EE Sse ep a ee eee eee 9 6 1 Front Panel Controls 6 1 1 Power Switch The Power switch for the system is located on the front lower right of the units It is a simple rocker switch on
25. Sonifier Cell Disruptor Model SLPe User s Manual EDP 100 214 254 Rev A BRANSON Ultrasonics Corporation 41 Eagle Road Danbury Connecticut 06813 1961 U S A 203 796 0400 BRANSON Manual Change Information At Branson we strive to maintain our position as the leader in ultrasonics plastics joining cleaning and related technologies by continually improving the designs circuits and com ponents in our equipment These improvements are incorporated as soon as they are devel oped and thoroughly tested Information concerning any improvements will be added to the appropriate technical doc umentation at its next revision and printing Therefore when requesting service assistance for specific units note the Release information found on the cover of this document and refer to the printing date which appears in the lower right corner of this page Patents and Copyright Copyright 2003 by Branson Ultrasonics Corporation Danbury Connecticut U S A All rights reserved Manual part number EDP 100 214 253 Initial Release printed August 2003 Branson Ultrasonics Corporation equipment is manufactured under one or more of the fol lowing U S Patents 4 249 986 4 315 181 4 363 992 4 551 690 4 651 043 4 786 356 4 973 876 5 095 188 5 435 863 Note Sonifier is a registered trademark of Branson Ultrasonics Corporation Danbury Connecticut U S A 100 214 253 Rev A May 2007 Sonifier Cell Disruptor Model SLPe
26. a cycle for its duration or Press and release to start a cycle press again to abort cycle 2 4 100 214 254 Rev A SLPe Cell Disruptor Chapter 2 Introduction to the SLPe Cell Disruptor User Manual Controls and Commands Left Arrow Key The Left Arrow key selects from right to left causing each digit in turn to blink and wraps back to the right The blinking digit is available to increment using the Up Arrow key In the configuration menu the Left Arrow key toggles the function between Off 0 and On 1 Up Arrow Key This key will increment a selected digit one count per press The display will increment appropriately for the selected parameter 2 2 2 Back Panel Connections Figure 2 3 Back Panel of SLPe Sonifier Cell Disruptor P O lj DA IEC Power In and Fuse RF Out J1 User Input J2 Unit Data Tag BRANSON 6 ULTRASONICS CORPORATION smanun E The back panel of the SLPe Cell Disruptor is equipped with An IEC type power cord connection for connecting the power supply to a grounded electrical outlet e It incorporates a fuse holder with a replaceable 5x20mm protective fuse The fuse is a glass slow blow type refer to the data tag for the fuse rating A3 pin RF connector to connect the power supply to the Converter A User external input connector female DB9F requires a cable with a 9 pin male DB9 connector 100 214 254 Rev A 2 5 Chapter 2 Introduction to t
27. age and shipping 20 F 29 C to 158 F 70 C Tuni Factory set no tuning necessary No user accessible adjust uning ments AC Mains Fuse 8A 250 V fast acting 5 x 20 mm Voltage Current 150W 100 120VAC 50 60 Hz 2A 200 240VAC 1A 100 214 254 Rev A 5 1 Chapter 5 Technical Specifications BRANSON Physical Description 5 2 Physical Description The following table outlines the physical characteristics of the Digital Sonifier Converter Weight with cable 1 1 lbs 0 5kg Converter Length 5 127mm Converter Diameter 1 5 38mm Converter to Microtip Stud M8 x 1 25 Horn Tip Diameters 0 091 0 125 and 0 250 2 3 6 4 mm depending on process needs Overall Dimensions Power Supply Weight 12 1 2 L x 7 1 2 W x 9 1 4 H 318x191x235 mm excluding RF out connector 5 8 16mm 150 Watt unit 14 Ibs 10 oz 6 5kg 100 214 254 Rev A SLPe Cell Disruptor Chapter 5 Technical Specifications User Manual System Performance Benchmark 5 3 System Performance Benchmark Each application and system configuration is slightly different System performance will vary when you change setup parameters and if your tools change This can affect the results of your experiments Creating a benchmark of your initial setup and performance can be useful at a later date in identifying a change in performance and can also help in recreating your exact initial setup The following
28. e horn or tip when ultrasonics are active When handling removing or attaching a tool or tip be sure that the ON OFF switch is set to OFF Touching the tool while the unit is on can result in serious personal injury DO NOT allow the tool to contact lab stands beakers test tubes or similar objects Microtip failure may result Breakage of glassware may result in the loss of a specimen DO NOT operate the equipment at more than 7096 amplitude Appropriate eye protection should be worn to prevent possible splash injury 100 214 254 Rev A 1 1 Chapter 1 Safety and Support BRANSON General Precautions 1 2 General Precautions 1 2 1 Intended Use of the System The SLPe Cell Disruptor can be used for Sample Prep Cell Lysing Disaggregation Emulsification Communition Extraction Dissolution Sonochemistry Homogenization Mixing Synthesis Catalysis Microencapsulation Hydrolyzation Degassing Cleaning Dispersion Disruption Atomization With the SLPe Cell Disruptor you can prepare an emulsion to 0 01 micron homogenize immiscible liquids polymerize some materials and depolymerize others 1 2 2 Safety Measures and Guards This manual contains operation instructions for the SLPe Cell Disruptor It contains information essential to the proper use and care of this equipment The manual contains notes warnings and cautions These are described as follows NOTICE Provides information that the reader should follow to prevent inconvenie
29. e interface surface on the emery cloth Grasp the part at the lower end near the surface to be reconditioned and lap the part in a straight line across the emery cloth NOTICE Do not apply downward pressure The weight of the part alone provides sufficient pressure Rotate the part 120 degrees 1 3 Stroke the part an equal number of times at each rotation 2 or 3 Pick up the part and lap it once or twice in the same direction Rotate the part 120 degrees and lap the part the same number of times as described above Rotate the part another 120 degrees and repeat the lapping procedure COON on Re examine the mating surface If necessary repeat steps 5 through 10 until you remove most of the con taminant This should not require more than two to three complete rotations for an aluminum horn or booster a titanium component may require more rotations 7 3 Troubleshooting Charts Use the following troubleshooting charts for possible problem conditions and resolutions The charts are based on the assumption that the proper setup and operation instructions have been followed and or the sys tem was working and then developed a problem 100 214 254 Rev A 7 3 Chapter 7 Maintenance Troubleshooting Charts Table 2 1 System Trouble Analysis Chart BRANSON Symptom Probable Cause Corrective Action Main power fuse fails or circuit breaker trips when system is plugged into electrical outlet Cordset has fa
30. ed before the next cycle Auto Reset Ar To set the mode for resetting all alarms select Off 0 to reset using the front panel Reset key or through the rear mounted external user I O connector J2 utilizing pins 1 and 7 When On 1 is selected a reset signal is not required All functions of the power supply are accessible Panel Trigger PT Refer to the following Table to set the method of starting a cycle for any mode of operation Table 6 1 Panel Trigger Pt Off 0 Pulse Start PS Start signal must come from User Input J2 and must be Off 0 held throughout the cycle When signal stops cycle ends Start signal can come from either the front panel Mem Panel Trigger Pt On 1 Pulse Start PS brane Keypad or the User Input J2 When a signal is On 1 applied 20 ms min and released the cycle will continue until the key is pressed again to Stop Start signal must come from User Input J2 When a signal Panel Ingger PD EVI Pose Sars is applied 20 ms min and released the cycle will con On 1 UP tinue until J2 is triggered again to stop Start signal can come from either the MEmbrane Keypad des a Start Stop key or J2 and must be held throughout the 0 cycle When key is released cycle ends 6 4 2 Control Menu After power up the display will go to the ready state and displays rdY ready There are two groups of addressable functions Mode and Set The current Mode LED will be on Pressi
31. ed hole 2 Thread the horn stud into the converter and tighten using wrenches The recommended torque is 220 inch pounds 24 85 Nm The tip must be installed clean and dry or the power supply may not tune and operate correctly 100 214 254 Rev A 4 7 Chapter 4 Installation and Setup Input Power Requirements BRANSON 4 5 Input Power Requirements The input power requirements for the SLPe Cell Disruptor are 117 VAC 50 60 Hz North American model 200 245 VAC 50 60 Hz Export models only The SLPe Cell Disruptor is equipped with an IEC type power cord connector The unit requires a single phase three wire 50 60 Hz power source To prevent the possibility of electrical shock always plug the SLPe Cell Disruptor unit into a grounded power source The system is fuse protected with a replaceable glass fuse 5x20mm slow blow type refer to the data tag on the system This fuse should never fail under normal operating conditions The fuse holder is found on the rear of the unit as part of the IEC power connector 4 6 Electrical Connections to Equipment All of the connections to the SLPe Cell Disruptor are made to the rear of the unit using industry standard connectors Refer to Figure 2 3 Back Panel of SLPe Sonifier Cell Disruptor on page 2 5 for connector loca tions 4 6 1 Power Cord North American units are shipped with a 3 conductor 117 Volt cordset NEMA 5 15P to IEC jack It con nects to an IEC t
32. een eee YOLOSNNOD SELLERS 1SOd ONIONIa ETL E NOlLdO NBL EOIN I Gre 79 7 05g ION H F N a m ETE ae Woo S 3 H ej Dias xa a Woo oa ze ON mm zx zm G3sn LON oor Ax opo slo 5 5 QB sje E slo aye SN aye CTI alo BB sis SN sis MN Eum slr sis on ab E els a ED Wl 3anindwv n MA ON woo Hu SN Esfisd afer Ho E i pes 6 6 sls EE HE 103135 119 3NVHBN3SN MH ON HH Wh dOISIINVIS MH TS sls BF 103188 300W WOO e Hf 1531 s s s s sis MH T ds ME 15m ele EN ele Q8H31OMINOO ele EEME als ON HE BE 5203113539 Bu a nn WOO Esrtsal wa Bajar Enid za QNVOS TOMINOO gg x s s Alddhs H3MOd s z ES ale ele ERIS ale ale SINOS MM v o vr eye ele le y zz nosne E O E HH EN A er oau ose ES DOT 5 2 z Eirtid Seen Sid Sif BIN iy NWS En V 31ON 33S hi aasn LON 31ndow 3 9 ee Ti AlddNS H3MOd weed 4 E ji Eje Box i ji ius 1233 E p pol o B e 2 HET He wawo T Mt M d i SE zu Tu BA z omar 9 vv ino dw z oos G3sn LON a a o aN ayog YIMOd ass Na ovi tajt auium g oonez E ba XB ey EF z AT8A3SSV Java tzer shi uim re ey shed er veer eb 8 Ba ela TANGO Tanu SiS cas 2 vr Ergo 3 a E m mosh _ Tango OS raini 3 me any E mul toa Els ms Nua 8 cami Shue MY M pz a E Le NUS Za IMS yl ur 2d es sio S a 7 id ANO EH g fa the e ino E Eid KINO MOOS e Azt AINOMODS Z 5 lt m 208 E aana a PANES Wand NUELA mus es CPE E 7 7 x aa 5 ELS Cima ino E TaImE NINO SLINN at au 6 s annaow o4d
33. hapter 6 4 1 3 Use the Up 9 arrow key to toggle between Off 0 and On 1 and apply to selected item as desired 4 Press ENTER to accept choices and exit the Configuration Menu Set Power Supply Wattage 1 Press TEST 5 while powering up All Set LED s 3 will blink 2 Set wattage to match power supply wattage 150 for SLPe 3 Press ENTER to go to ready mode 6 5 1 SLPe in Continuous Mode Process Sequence 1 Turn On power wait for display 1 to show Ready rdY 2 Select Mode 2 Continuous 2A Amplitude LED 3D will light 3 Set Amplitude from 10 to 100 using arrow keys 4 Press ENTER 5 S Send Start pulse by shorting pins 2 and 6 of external user I O J2 to start cycle or by pressing the Start top key as per configuration NOTICE entering an illegal value will generate 3 beeps The system will not accept out of range parameters 100 214 254 Rev A 6 7 Chapter 6 Operation BRANSON Operational Sequence 6 5 2 SLPe in Time Mode Either Low min sec or High hr min 1 Turn On power wait for display 1 to show Ready rdY 2 Select Mode 2 Time 2B The On 3A Off 3C and Amplitude LED s 3D will light 3 Set the On time Off time and Amplitude using the arrow keys 4 Press ENTER 5 Send Start pulse by shorting pins 2 and 6 of external user I O J2 to start cycle or by pressing the Start Stop key as per configuration 6 5 3 SLPe in Energy Mode 1 Turn On power wa
34. he SLPe Cell Disruptor System Features BRANSON 2 3 System Features The SLPe Cell Disruptor includes the following features Front panel system On Off switch Separate front panel Stop and Pause buttons for experiment cycles Power output of up to 150 Watts 92 132 Volt and 180 253 Volt models available factory set Front panel LED display showing parameter settings alarms and messages Membrane front panel with numeric keypad for parameter entry and function selection Digital parameter setting with valid parameter range checking Pulse or Continuous operation Digital Timer Automatic end of cycle audible alarm Factory tuned 40 kHz ultrasonic design requires no user adjustment User External Input provides ability to remote start seek and overload Alarm Reset Also can monitor Weld On Ready and Alarm For additional feature information please contact your Branson representative 2 6 100 214 254 Rev A SLPe Cell Disruptor Chapter 3 Delivery and Handling User Manual Delivery and Handling Chapter 3 Delivery and Handling 3 1 Delivery and Handling The Digital Sonifier has no special handling constraints On receipt of your SLPe Sonifier take the follow ing steps Inspect the carton for signs of damage Open the carton and locate the packing list Carefully unpack the components and check them against the packing list Save all packing materials in case the equipment needs to be shipped Inspect the component
35. ibrating horn or place the vibrating horn against solid objects Touching or holding the horn can result in burns or injury and contacting solid materials with the vibrat ing horn or tip can cause breakage DO NOT run the microtip above an Amplitude setting of 7096 Doing so can cause damage to the microtip assembly Alarms When the system encounters an error condition an error message is displayed on the front panel of the SLPe Sonifier unit and the Reset LED will flash The following table describes the error messages the conditions that cause them and the actions you should take when they are displayed Table 6 1 Error Messages Message Cause Take This Action Er OL Power Supply Overload Depending on configuration Reset the power supply Refer to 6 4 1 Configuration MenuTurn off unit and power up again If the message repeats check 96 Peak Power to verify it is less than 100 If this does not resolve the problem check your RF connection and the converter If unable to rid Er OL at this point call your local Branson representative or send to Bran son Authorized Service Center for repair Er rF Er SS NOVRAM Failure Remote Start Signal still active either when the unit is turned on or if it is not released at the end of a cycle Turn power off and back on If the mes sage repeats call your local Branson rep resentative or send to Branson Authorized Service Center for repair
36. ihe gt te Gell Disruptor iow Prepare the Sonifier to operate if it was not previously 1 ing the instructions in this manual E assembled Connect the microtip to the converter Verify that the system passes all its self tests observ Connect the RF cable to the power ing that there are no error messages on the front 2 supply Turn the Unit On and observe panel display the self test displays The SLPe Cell Disruptor advances to the Ready mode and shows the normal Ready display Adjust the Amplitude control to Ensures that ultrasonic energy will be at some mid 3 approximately 5096 observe the range value and will not cause damage Refer to value on the front panel display Table 4 3 for amplitude information Verify that the Horn is not touching anything 4 Press and hold the Test button on Verifies the ultrasonic output of the system You may the front panel The test will run until hear a soft high pitched sound the test key is released Observe the front panel display If the system showed readings on tha display anno me dest Verification that the SLPe Cell Disruptor is operating 5 you may either proceed with your experiments or Turn the unit off and is ready to be set up for your experiment Use the following charts to determine the correct system settings for the tools you are using Table 4 3 Approximate Microtip Amplitudes in Microns Ses Amplitude Control Settings Microtip EDP No
37. iled Replace cordset Power Switch has failed Line filter has failed Return for repair Display screen does not appear when unit is turned on Fan does not operate System is unplugged or main power is missing Correct power problem Unit fuse has blown it should not under normal conditions Cordset has failed Replace fuse Replace cordset Power Switch has failed Line filter has failed Unit failed due to connection to incorrect input voltage Return for repair Fan does not operate when system is turned on Display screen appears Fuse fails when system is turned on Fan motor has failed Fuse is under rated Mains Voltage is incorrect Fan motor has failed Power supply module has failed Unit failed due to connection to incorrect input voltage Return for repair Verify voltage source is correct Damage may occur if connected to wrong voltage source Replace fuse with cor rect value and retry or return for repair Ultrasonic power is not delivered to the horn Unusual noise from tool when ultrasonics are on Ultrasonic power supply module has failed Digital controls have failed Tool is loose or it is contact ing a solid object Return for repair Reposition tool Remove examine and clean tool reinstall tool Tool has failed Replace tool 7 4 100 214 254 Rev A
38. it for display 1 to show Ready rdY 2 Select Mode 2 Energy 2C The Joules 3B Off 3C and Amplitude LED s 3D will light 3 Set the energy in Joules Off time and Amplitude using the arrow keys 4 Press ENTER 5 Send Start pulse by shorting pins 2 and 6 of external user I O J2 to start cycle or by pressing the Start Stop key as per configuration 6 5 4 SLPe in Time Mode with Pulse Either Low min sec or High hr min 1 Turn On power wait for display 1 to show Ready rdY 2 Select Mode 2 and press until Pulse 2E and Time 2B are both selected The On 3A Off 3C Amplitude 3D and Experiment Time 3E LED s will light 3 Set the Time On Off time Amplitude and Exp time using the arrow keys 4 Press ENTER Autotune Label Covers Pot uration 6 5 5 SLPe in Energy Mode with Pulse 1 Turn On power wait for display 1 to show Ready rdY 2 Select Mode 2 and press until Ground Detect 2E and Energy 2C are both selected The Joules 3B Off 3C Amplitude 3D and Experiment 3E LED s will light 3 Set the energy in Joules Off time Amplitude and Experiment time using the arrow keys 4 Press ENTER 5 Send Start pulse by shorting pins 2 and 6 of external user I O J2 to start cycle or by pressing the Start Stop key as per configuration 6 8 100 214 254 Rev A SLPe Cell Disruptor User Manual 6 6 Chapter 6 Operation Alarms Do NOT touch the v
39. l Warranty 1 4 Warranty Refer to the Terms and Conditions of Sale found on the back of your Invoice for information about the product Warranty issued of your Branson products If you have any questions please contact your Branson representative The product warranty information is summarized below WARRANTY When used in accordance with written instructions and under normal operating conditions Branson Ultra sonics Corp equipment is guaranteed to be free of defects in MATERIAL and WORKMANSHIP for two 2 years from the date of original delivery by BRANSON or by an authorized representative Any unit which proves defective during the stated period will be repaired free of charge or replaced at the sole dis cretion of Branson Ultrasonics Corp E O B Danbury Connecticut U S A or an authorized repair station as advised by BRANSON provided the defective unit is returned properly packed with all transportation charges prepaid A limited warranty as specified may apply to certain components of the equipment WARRANTY EXCEPTIONS This warranty shall not apply to equipment subjected to misuse improper installation alteration neglect accident or improper repair This warranty is limited to the original purchaser and is not transferable Tools fabricated by Branson for use in equipment described in this manual are manufactured to exacting parameters Using altered or modified tools or tools otherwise unqualified by Branson can produce u
40. m intermittent How often does it occur How long before it occurs if you are just power ing up 2 Is your equipment in a remotely operated system If so is the problem related to Start Stop control or interaction with PLC s or other devices etc 3 If the problem is with an external signal or output which one If known include plug pin e g P29 pin 3 4 What are the setup parameters 5 What is your application e g continuous pulse etc 6 Name and phone number of the person most familiar with the problem 7 Notify Branson prior to shipping the equipment To return equipment to Branson you must first obtain an RGA number from Branson or the shipment may be delayed or refused 8 For equipment not covered by warranty include a purchase order for the repair costs to avoid delay 9 Pack carefully in original packing material to avoid damage in shipment 10 Return general repairs by any desired method Send priority repairs by air freight 11 Prepay the transportation charges FOB Danbury Connecticut U S A Notes 100 214 254 Rev A 1 7 Chapter 1 Safety and Support BRANSON How to Contact Branson Returning Equipment for Repair to Danbury facility NOTICE To return equipment to Branson you must first obtain an RGA number from Branson or the shipment may be delayed or refused Call the Repair department to obtain a Returned Goods Authorization RGA number If requested the Repair department can
41. nce Advises the user of a hazard that can cause eguipment damage or personal injury Alerts the reader to a hazard that can result in severe personal injury Do NOT disregard a WARNING 1 2 100 214 254 Rev A SLPe Cell Disruptor Chapter 1 Safety and Support User Manual General Precautions 1 2 3 Safe Operation Setup and Operation instructions are found in Chapter 6 of this manual For safe operation please ensure that all people using this equipment follow those instructions and observe all CAUTION and WARNING notices 1 Make sure that the equipment is properly grounded DO NOT operate if it is not 2 Do not allow the tool to contact lab stands beakers etc or tool failure may result 3 Periodically test the equipment as described in 4 8 Ultrasonic Test and 5 3 System Performance Benchmark Although the SLPt SLPe Cell Disruptor operates outside the normal range of human hearing some appli cations can create audible noise above 85dB If an uncomfortable level of noise is present the operator should wear ear protection for safe operation Appropriate eye protection should be worn when operating the SLPe Cell Disruptor to prevent possible splash injury originating in the solution Never touch the tool when ultrasonics are active Touching the tool while the unit is on can result in serious injury When you handle remove or attach a tool always make sure that the ON OFF switch is OFF 1 2 4 Setting Up the Workplace
42. ndue stresses that may damage the equipment Equipment failures resulting from using unqualified tools are not covered by the Branson warranty Microtips are designed to give maximum mechanical energy output Since they operate close to the stress limits of titanium Branson cannot guarantee microtips against failure CONTACT YOUR BRANSON REPRESENTATIVE OR BRANSON ULTRASONICS CORPORATION DANBURY CONNECTICUT SHOULD YOU HAVE ANY QUESTIONS CONCERNING TOOL QUALIFICATION 100 214 254 Rev A 1 5 Chapter 1 Safety and Support How to Contact Branson BRANSON Make a copy of this page and return it with the product 1 5 How to Contact Branson The mailing address and telephone information for Branson is as follows Branson Ultrasonics Corp 41 Eagle Road P O Box 1961 Danbury Connecticut 06813 1961 U S A Sales Support 800 732 9262 1 5 1 Before Calling Branson for Assistance This manual provides information for troubleshooting and resolving problems that could occur with the equipment see Chapter 7 If you still require assistance Branson Product Support is here to help you The following questionnaire lists the common questions you will be asked when you contact the Product Support department to help identify the problem Before calling determine the following information Your company name and location Your return telephone number w N Have your manual with you If troubleshooting a problem refer to
43. ng the Mode button will sequence to the next mode in a continuous loop The selected mode s LED will come on and the corresponding Set parameters associated with the mode will light 100 214 254 Rev A 6 5 Chapter 6 Operation Menu Navigation The following table shows the relationship between the Mode and Set parameter Table 6 2 BRANSON Mode Set available parameters Continuous Amplitude Time minutes seconds On Off Amplitude Time hours minutes On Off Amplitude Energy Joules Off Amplitude Time minutes seconds and Pulse On off Amplitude Experiment Time Energy and Pulse Joules Off Amplitude Experiment Time An error will generate 2 beeps entering an illegal value will generate 3 beeps The system will not accept out of range parameters 100 214 254 Rev A SLPe Cell Disruptor Chapter 6 Operation User Manual Operational Sequence 6 5 Operational Sequence NOTICE While Configuration Menu parameters can be specified each time the unit is powered up the default factory settings are correct for most applications and are enabled at power up If there is a need to re configure press and hold the ENTER key 6 while powering up To configure the SLPe 1 Press and hold Enter key while turning on power 2 When in the Configuration Menu use the Left 8 arrow key to navigate to either Pulse Start Panel Trig ger or Auto Reset as required C
44. ntact name information 1 8 100 214 254 Rev A SLPe Cell Disruptor Chapter 2 Introduction to the SLPe Cell Disruptor User Manual Overview of the SLPe Chapter 2 Introduction to the SLPe Cell Disruptor 24 Overview of SLPe 1 2 2 Controls and Commands 2 2 2 1 SLPe Cell Disruptor Keypad Display Description 3 2 2 2 Back Panel Connections 5 23 System Features 6 2 1 Overview of the SLPe The SLPe Cell Disruptor is often used by laboratory personnel in the medical and chemical process fields The system consists of the power supply which includes all controls the converter and the tapered microtip Figure 2 1 SLPe Sonifier Cell Disruptor 100 214 254 Rev A 2 1 Chapter 2 Introduction to the SLPe Cell Disruptor Controls and Commands BRANSON The digital controls on the SLPe Cell Disruptor allow for accuracy and repeatability of control settings Application parameters are entered into the power supply through the keypad on the front panel of the unit External signals are initiated via J2 9 pin connector on the back of the power supply To simply generate a start signal a J911 Start cable 8 EDP101 240 020 can be used To control or monitor other signals Reset Seek Alarm Ready a J913 Start cable 25 EDP101 240 072 must be used Reset and Start func tions can also be performed from the front panel Fu
45. ours 59 minutes Energy from 1 joule to 9999 joules Off Time from 1 sec to 99 minutes 59 sec Amplitude from 10 to 100 Experiment Time from 1 minute to 99 hours 59 minutes Time On LOW Pulse Experiment from 1 second to 99minutes 59 seconds Time On HIGH Pulse Experiment from 1 minute to 99 hours 59 minutes Set Energy Pulse Experiment from 1 to 9999 Joules 4 Reset Resets an alarm Pressing Reset during parameter entry will restore the original value to the parameter being modified and then return the system to ready At this time the power supply is also reset and cleared of any alarms 5 Test Sets up resonant frequency This key is only active when the system is ready Pressing Test will turn on sonics and hold them on until the key is released Test performs run seek and store function causing the power supply module to test for the resonant frequency of the stack at 10 amplitude for a predetermined time While in Test mode the Mode LED s will be off the Amplitude LED will be on and the lower colon will be blinking When completed the Mode LED s will be restored the amplitude LED and blinking half colon will be off 6 Enter Confirms a selection setting This includes the configuration menu 7 Stop Start Starts or Aborts a cycle Set up in Configuration menu to either a Pulse Start 0 Press and hold to run a cycle or Pulse Start 1 Press and release to run a cycle Left Arrow Indexes active digit right to
46. r any given application more power results when a tool of higher amplitude or larger radiating surface is used or when any tool is driven at higher amplitude by increasing the amplitude By setting various operation parameters you can precisely control the way in which ultrasonics are applied to the sample You can e Specify the time duration of the experiment Adjust the amplitude setting between 10 and 70 of maximum amplitude microtip 70 max imum Preventexcessive temperature increase in the sample by setting ultrasonics to operate in Pulse mode or in Pulse Pause mode Bring a sample to a desired temperature and hold it there varying by only a few degrees for a desired duration using the Pulse Pause mode 2 2 Controls and Commands This section describes the controls and commands that you use to operate the SLPe Cell Disruptor A detailed description of how and when to use each front panel control the valid formats for the data that you enter and the response you receive from the system when you use each of these controls is provided in Chapter 6 Operations The SLPe Cell Disruptor is equipped with a keypad and LED display on the front panel of the unit With the keypad you can set functional modes of operation and input digital parameters Availability of the various functions depends on the mode or state of the system If you attempt to use a function that is not available you will be alerted by a beep 2 2 100 214 254 Rev
47. rode Tip erosion is a side effect of the cavitation process that occurs when liquids are exposed to ultrasonic energy The rate of erosion depends on the intensity of power applied the corro siveness of the liquid being treated and the amount of use Periodically inspecting the tip will help you recognize erosion early As erosion progresses the color of the tip changes from its original polished appearance first to light grey and then to dark grey Concentric rings begin to appear and finally the tip becomes rough and pitted resulting in loss of power output As it erodes the tip can also introduce metal particles into the solution causing it to darken or discolor Eventually erosion may become significant at which point the tip will require replacement 100 214 254 Rev A 7 1 Chapter 7 Maintenance BRANSON Reconditioning the Converter Tool Interface Power Output Loss There are several conditions that can cause a decrease in or loss of power output including operating with a faulty power supply or poor electrical connection operating with a loose tool converter connection and operating with a cracked or corroded tool If your unit indicates a decrease in power output first check the Converter Cable connections then take the following steps to ensure that the tool assembly is not loose or cracked or corroded Fretting corrosion refers to a black crusty build up resulting from friction between metal parts that appears
48. rther information about these signals can be found in Section 4 6 2 All other functions can only be performed from the front panel The SLPe Cell Disruptor s power supply converts AC line voltage to 40kHz electrical energy This high frequency electrical energy is fed to a converter where itis converted to mechanical vibrations The heart of the converter is a lead zirconate titanate electrostrictive element which when subjected to an alternating voltage expands and contracts The converter vibrates in a longitudinal direction and transmits this motion to the tool immersed in the solution which causes cavitation The implosion of microscopic cavities in the solution results causing the molecules in the medium to become intensely agitated The ultrasonic vibra tions transmitted through the tool can also be applied directly to a solid workpiece such as tissue through the tool that are attached to the converter The SLPe Cell Disruptor is a constant amplitude device As the load or pressure on the tool face increases the power supply develops more power to maintain the amplitude for any given output control setting When the tool is operated in air it is subjected to minimum pressure and minimum power is required to maintain amplitude The load increases when the tool is immersed in a liquid the more viscous the liquid the higher the load and the more power developed If a cell that can be pressurized is used even more power is developed Fo
49. s for any damage that may have occurred during shipping Report all shipping damage to your carrier OV OX Ier 100 214 254 Rev A 3 1 Chapter 3 Delivery and Handling BRANSON Delivery and Handling For Your Notes 3 2 100 214 254 Rev A SLPt Cell Disruptor Chapter 4 Installation and Setup User Manual Installation Checklist Chapter 4 Installation and Setup 4 4 Installation Checklist 1 4 2 System Block Diagram 2 4 3 System Component Description 2 43 1 Standard Components 2 43 2 Tools 3 43 3 Accessories 4 4 4 Assembling the Equipment 6 44 1 Setup Procedure 6 4 4 2 Connecting Microtips and Converters 7 45 Input Power Requirements 8 4 6 Electrical Connections to Equipment 8 46 1 Power Cord 8 4 6 2 User l O Connection 9 4 7 Guards and Safety Equipment 9 48 Ultrasonic Test 10 4 1 Installation Checklist To install the SLPe Cell Disruptor you will need these items SLPe Cell Disruptor unit Power cordset Ultrasonic converter Tool Wrench Set Any accessories shipped with the unit 100 214 254 Rev A 4 1 Chapter 4 Installation and Setup BRANSON System Block
50. steps are used only to record system performance not your experiment results NOTICE Make copies of the following page and keep it on file for future reference Use the following steps to create and record a benchmark for your exact setup Step Action to Save a Benchmark 1 Make a copy of the SLPe Setup Form provided on the following page 2 Identify your experiment type on the form for your own reference Set up the Digital Sonifier be ready to run and prepare your experiment 3 Turn the SLPe system On 4 Select Mode of operation Set On time Off time and Energy joules SLPe only 5 Set your desired Amplitude With the microtip submersed in your solution press the Start key located 6 on the lower right hand side of the SLPe keypad Note any special immer sion comments depth how it is supported etc Press the Down Arrow key on the keypad and view the POWER reading the 7 value is displayed on the LED display Record the POWER reading on the form 8 Note any special adjustments settings operating modes or other system variables that will be helpful at a later time in re creating your setup Turn the system off Record the serial numbers and microtip information of the SLPe unit and any special equipment 10 Place the filled in form in a safe place for future reference 100 214 254 Rev A 5 3 Chapter 5 Technical Specifications System Performance Benchmark BRANSON Branson S
51. ultiple times until experiment time expires or a Stop condition is received Energy Mode Ultrasonics are applied to the workpiece until either the energy you specify is attained or until the timeout time is reached SLPe only 60 seconds There is no indicator for timeout In Pulse Experiment Energy ultrasonics can be turned on and off multiple times The On duration is con trolled by the time it takes to reach the Joules of energy that are set This will continue until experiment time expires or a Stop condition is received 6 4 Menu Navigation 6 4 1 Configuration Menu This menu alllows the operator to configure the unit to specific preferences or operating methods Within the Configuration Menu Pulse Start Panel Trigger and Auto Reset can be turned On or Off Press and hold the ENTER key while powering up to enter the menu 6 4 100 214 254 Rev A SLPe Cell Disruptor Chapter 6 Operation User Manual Menu Navigation Pulse Start PS To set the method of trigger switch duration select Off 0 if you desire to hold the trigger signal throughout the duration of the process cycle If the start signal is lost the cycle is aborted and no alarm is produced Select On 1 if you wish to start the cycle with a pulse minimum duration of 10ms After sending the pulse the sonics will remain on for the duration of the cycle Pressing again will abort the current process cycle NOTICE In both modes the start signal must be remov
52. wanima mwm ami zaman 1 3 OO 5 4 SOtUpi iiie ee e aid 4 1 5 3 setup procedure ww wwwesemanamumanwmwamuwa ziwa tenentes nennen nene 4 6 SEPUSEPe Setup Form diee ere taret ekanda 5 93 Soundproof DOK atinada cleaned 4 5 ELE inetr ntnntnentrnntsnnnssrensennnsnns 4 2 SUPPOM aaa ar dio 1 1 system intended Ui eed etude gi cid 1 2 system block diagram ssssssssseseeeeennnennns 4 2 system component description 4 2 T technical specifications for the Digital Sonifier 5 1 ELE PME 4 10 Timed Mode ip per aia cea ai 6 4 UID CFOS ION oars M ie E 7 1 tips CONMECTING EE 4 7 troubleshooting icio ee FT Ld n eo do ELE Reda 7 1 U ultrasonic test te eoe ted aie Gaetan 4 10 User I O sities M 4 9 W warranty statement kisii Bwa walieka eei earn khaki kata a Ta da are Funera ds 1 5 workplace Setting Up ir oet ee tet tte a B e ae eee 1 3 index iv 100 214 254 Rev A
53. ype connector on the rear of the unit The plug end connects to your main voltage recepta cle which should be properly fused depending on your site requirements It requires a conventional NEMA 5 15R receptacle for installation Export units are shipped with a standard Harmonized European cordset having an IEC type jack If your cord set does not match your main power receptacle verify that you have the correct voltage available Do not connect the system if the voltage rating of the unit is incorrect for your location as this can damage the unit 4 8 100 214 254 Rev A SLPt Cell Disruptor User Manual Chapter 4 Installation and Setup Guards and Safety Equipment 4 6 2 User I O Connection The SLPe Cell Disruptor is equipped with a standard external connection to allow you to design and connect your own custom interface for controlling the unit The User I O interface can be useful when you need to activate the Sonifier remotely for example when the operator must start and stop the unit from another room for safety reasons Table 4 2 User I O Pin Out DB9F provided for customer designed interface DB9F Pin Function Signal 1 Reset Input 2 Start Input 3 Weld On Output 4 Alarm Output 5 Ready Output 6 Start Source Input 7 Reset Alarm Ready Weld On Common 8 Seek 24 Volts Input 9 Seek Ground 4 7 Guards and Safety Equipment Although the SLPe Cell Disruptor operates outside the
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