User Manual Picus® & Picus® NxT Electronic Pipette
Contents
1. Alphabets symbols Active in memory settings and in setup settings for entering letters numbers or symbols Right Softkey ADV Displays the additional modes for the activated main mode OK Confirms the setting selection and exits the MENU EDIT or ADV functions or the current display CLEAR Deletes the characters from User ID or the names given to the memory locations Hotkey All models include 10 memory places for the favourite and most used pipetting settings 3 2 Pipetting Modes The Picus Picus NxT electronic pipette has eight 8 main pipetting modes The Picus NxT has seven 7 additional modes while the Picus2 has six 6 additional modes Pipetting Modes available in all Picus amp Picus NxT models Pipetting Reverse Pipetting Multi Dispensing Manual Pipetting Diluting Sequential Dispensing Multi Aspiration suas ass IS Sa posse eee is Titration Repeted Blow out only in Picus NxT Additional Modes ADV to be used in conjunction with the pipetting mode Tracker Mixing Counter Excess Volume Auto Repeated Fast n Adjustment Dispensing Blow out X Dispensing v v v v v v v v v Sf v v v v v v 3 2 1 Pipetting Forward Pipetting Pipetting mode aspirates and then dispenses the selected volume of liquid Recommended for aqueous liquids liquids containing small amounts of detergent or proteins and solvents To select Pipetting mode 1 Select MENU by pre2. 5000 1000 10 120 60 12 300 150 30 1200 600 120 10 120 60 12 300 150 30 1200 600 120 Systematic Error 0 90 1 00 2 50 0 40 0 60 2 00 0 40 0 60 1 50 0 40 0 60 1 50 0 50 0 80 1 00 0 60 1 20 3 00 0 90 1 50 4 00 0 50 0 70 2 00 0 50 0 70 2 00 0 50 1 00 2 00 0 90 1 50 4 00 0 50 0 70 2 00 0 50 0 70 2 00 0 50 1 00 2 50 ul 0 09 0 05 0 025 0 48 0 36 0 24 1 2 0 9 0 45 4 00 3 00 1 5 25 00 20 00 5 00 60 00 60 00 30 00 0 09 0 075 0 04 0 6 0 42 0 24 L9 1 05 0 6 6 00 6 00 3 00 0 09 0 075 0 04 0 6 0 42 0 24 15 1 05 0 6 6 00 6 00 3 00 Random Error 0 40 0 70 1 50 0 15 0 20 1 00 0 15 0 20 0 80 0 15 0 20 0 50 0 15 0 20 0 40 0 20 0 30 0 60 0 50 0 80 3 00 0 20 0 30 1 50 0 20 0 30 1 00 0 20 0 30 1 00 0 50 0 80 3 00 0 20 0 30 1 50 0 20 0 30 1 00 0 20 0 30 1 00 ul 0 04 0 035 0 005 0 18 0 12 0 12 0 45 0 3 0 24 1 5 1 0 0 5 7 5 5 00 2 00 20 00 15 00 6 00 0 05 0 04 0 03 0 24 0 18 0 18 0 6 0 45 0 3 2 4 1 8 1 2 0 05 0 04 0 03 0 24 0 18 0 18 0 6 0 45 0 3 2 4 1 8 1 2 33 9 2 Speed Table Speed is measured in Pipetting mode with the maximum volume Speed can be adjusted in all main pipetting modes individually for aspiration and dispensing Speed range is from 1 slow to 9 fast Single channel pipettes speed in seconds Speed 10 ul 120 u
3. either a hold the operating button down and then release it for reaspiration OR b press the left Softkey for NO and start from the beginning 5 o discard the excess volume and to empty the tip press the operating button 6 Eject the tip by pressing the electronic tip ejector button Additional modes Excess Adjustment timed Automated Dispensing and Tracker can be used in conjunction with Multi Dispensing Excess Adjustment can be used to set the excess volume Timed Automated Dispensing dispenses the liquid automatically without the need to press the operating button Tracker advises the user in microplate Dispensing by displaying the location for the next dispensing For more information on using additional modes see chapter 3 2 9 NOTE Only one additional mode can be selected at a time 3 2 4 Manual Pipetting In Manual Pipetting the piston movement in aspiration and dispensing is controlled manually turning the adjustment wheel It is ideal for measuring reagents and in applications in which the pipetting speed needs to be controlled manually To select Manual Pipetting mode 1 Select MENU by pressing the left Softkey 2 Usethe adjustment wheel to select Manual Confirm the setting by pressing the operating button or the Softkey for OK To edit the parameters volume speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highligh
4. Diluting modes The volume for mixing can be selected freely up to the maximum volume of the pipette To set the parameters volume manual automatic 1 Once you have selected the Mixing mode the display shows the volume used for mixing 80 of the volume to be dispensed 2 Turn the adjustment wheel to set the desired volume for mixing 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and the pipette simultaneously exits the editing mode OR b the middle Softkey for NEXT when you can move on to select manual mixing or automatic mixing with the number of mixing cycles 4 f you chose 3 b then turn the adjustment wheel to select Manual or the number of automatic dispensings 5 Confirm the setting by pressing the operating button or the right Softkey for OK when you simultaneously exit the editing mode To activate deactivate mixing 1 Start the main mode in question per the instructions given earlier and once the display tells you to Start mixing a press and hold down the operating button for the duration of manual mixing OR b press the operating button once to activate the automatic dispensing cycles OR c press the left Sofkey for NO to exit Mixing 2 You may pause the mixing by pressing the operating button during the mixing cycle By pressing it again mixing continues 3 You may also exit mixing during the mixing cycle by pressing the left Softkey for QUIT 4 Continue by emptying
5. ON NN OOO Of RA WwW C2 WW C0 CO CO CO CO CO CO CO CONO EN EN EN EN EN EN NONO NONO ENDED END END END EM N NEN we HBP PsP er er Se Pe Se ea eS OM BPW NM HH KH QD QD QD d dq du CO DW OeNN EN ODO OHNO HP HW WW NM H KH OO OO On LH Hi ws Nw KH oco 1 Introduction The Sartorius Picus Picus NxT electronic pipette of the Biohitfarmily brings ergonomics into a completely new level with its exceptionally lightweight and compact design and its ease of operation This pipette operating with the air displacement principle has been designed by an experienced R amp D team in co operation with laboratory personnel and ergonomists to create instruments for safe and comfortable pipetting with versatile functionality The Picus Picus NxT is available in single and multichannel models 8 and 12 channel Tip attachment and ejection is made safe and comfortable with the Optiload tip loading feature together with the electronic tip ejection function To improve safety by reducing the risk of contamination replaceable Safe Cone Filters can be used in all Picus Picus NxT models greater than 10 ul The fully electronic operation the unique DC motor electronic brake and piston control system guarantee excellent accuracy and precision The light and easy to use adjustment wheel on top of the pipette enables fast selection of the desired volume Picus NxT follows in the footsteps of the design awarded Picus which won th
6. and the accuracy requirements placed on the pipette ISO 8655 1 4 4 Replacing the Battery It is recommended that the Picus Picus NxT battery is replaced only by an authorised Sartorius service provider Please contact your nearest Sartorius representative 4 5 Replacement Parts To replace broken or worn parts Sartorius offers a full range of spare parts Always contact the manufacturer or manufacturer s authorised representative for original spare parts for your Sartorius pipette 4 6 Storage When not in use it is recommended that the Picus Picus NxT electronic pipettes are stored on the charging stand During long storage periods several months it is recommended that the charging stand is disconnected from the mains power outlet and the pipette is turned off by pressing the power button on the top of the pipette 29 30 5 Warranty Sartorius products are meant to be used as described in this user manual Picus Picus NxT electronic pipettes are covered by warranty for two years against defects in workmanship and materials The battery is not covered by our warranty ANY WARRANTY WILL HOWEVER BE DEEMED AS VOID IF THE FAULT IS FOUND TO HAVE BEEN CAUSED BY MISTREATMENT MISUSE UNAUTHORIZED MAINTENANCE OR SERVICE OR NEGLIGENCE OF REGULAR MAINTENANCE AND SERVICE ACCIDENTAL DAMAGE INCORRECT STORAGE OR USE OF THE PRODUCTS FOR OPERATIONS OUTSIDE THEIR SPECIFIED LIMITATIONS OUTSIDE THEIR SPECIFICATIONS CONTRA
7. pressing the operating button Empty the tip by pressing the operating button Eject the tip by pressing the electronic tip ejector button CE M M ue Additional modes Mixing and repeated Blow out with Picus9 NxT can be used in conjunction with Diluting Mixing mixes the liquids manually or automatically The mixing volume can be adjusted according to the pipette s maximum volume Repeated Blow out helps to remove the residual liquid from the tip if the liquid is such that normal dispensing does not completely empty the tip For more information on using additional modes see chapter 3 2 9 3 2 6 Sequential Dispensing Sequential Dispensing dispenses repetitively selected volumes in any desired order This is a useful mode for example for diluting series and making calibration curves To select Sequential Dispensing mode 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Seq Disp Confirm the setting by pressing the operating button or the Softkey for OK The latest settings used will be shown To edit the parameters number of aliquots aliquot volumes speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highlighted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultaneously exit the editing mode OR b th
8. provider or distributor 4 2 Sterilizing The lower parts of Sartorius electronic pipettes can be sterilized by autoclaving UV or by using disinfectant or decontaminant liquids such as 70 ethanol 60 isopropanol mild detergent or similar Always make sure of the chemical compatibility between the materials of the pipette and the disinfectant or decontaminant liquid Always follow the autoclaving instructions below 4 2 1 Autoclaving Lower parts of Sartorius electronic pipettes are autoclavable excluding the lower parts of 1200 ul multichannel pipettes Please see the autoclaving symbol printed on the lower part of the multichannel pipettes to make sure the lower part is autoclavable Autoclaving instructions 1 Remove the Safe Cone Filter if attached 2 Unscrew the lower part by turning the connecting collar and the dispensing head counter clockwise until it comes off 3 Sterilize the part at 121 C 1 bar overpressure for 20 minutes 4 Let the parts cool and dry before reassembling NOTE The lower parts of multichannel pipettes can be autoclaved as one unit or separately as individual parts 2 28 4 2 2 UV Sterilization Sartorius electronic pipettes are made of UV resistant materials The UV resistance of the products has been tested in continuous UV light under the following test conditions which we also recommend to be applied in UV sterilization in labs Wave length Shortwave ultraviolet UVC Powe
9. the tip 18 Excess Adjustment Excess Adjustment can be used to set the excess volume with Reverse Pipetting Multi Dispensing and Sequential Dispensing modes where excess volume is used The excess volume varies according to the volume range of the pipette To set the excess volume 1 Turn the adjustment wheel to select the desired volume after the additional mode has been activated You may also press the middle Softkey for DEFAULT to choose the default excess volume 2 Confirm the selection by pressing the operating button or the right Softkey for OK Automated Dispensing Automated Dispensing dispenses the aliquots automatically in timed intervals with Multi Dispensing mode The user does not need to press the operating button for each dispensing The time period between dispensings can be set from 0 1 to 9 9 seconds To set the delay 1 Turn the adjustment wheel to select the desired delay right after the additional mode has been activated 2 Confirm the selection by pressing the operating button or the right Softkey for OK Fast Dispensing Fast Dispensing can only be used in the Titration mode In Fast Dispensing the first selected portion of the total volume is dispensed automatically and the remaining volume is dispensed manually To set the volume for Fast Dispensing 1 Turn the adjustment wheel to select the desired volume right after the additional mode has been activated 2 Confirm the selection by pre
10. 9 755 95 200 Business ID FI2441885 8 Internet site www sartorius com Contact us lhinfo finland sartorius com Factory in Finland Tietokatu 4 87400 KAJAANI FINLAND Contact Details Sartorius Liquid Handling Oy Laippatie 1 00880 Helsinki Finland Phone 358 9 755 951 lhinfo finland sartorius com Sartorius Lab Instruments GmbH Weender Landstrasse 94 108 37075 Goettingen Germany Phone 49 551 308 0 Fax 49 551 308 3289 www sartorius com The status of the information specifications and illustrations in this manual are subject to change Sartorius reserves the right to make changes to the technology features specifications and design of the equipment without notice All trademarks are Sartorius property unless otherwise stated Patents granted or pending 4 www sartorius com Publication No SUL6001 e141103 Order No 85032 542 12 Ver 11 2014 Sartorius Liquid Handling Oy
11. Adjustment function is highlighted Confirm the selection by pressing the operating button or the Softkey for OK 4 Use the adjustment wheel to select ADJ1 ADJ2 or ADJ3 memory location Confirm the selection by pressing the operating button or the Softkey for OK 5 The pipette asks if you wish to set the custom adjustment values Confirm the setting by pressing the operating button or the right Softkey for OK If you do not wish to set the data press the left Softkey for NO 6 If you confirmed the setting ADJ1 ADJ2 or ADJ3 is now displayed on the lower left corner per your selection and the pipette simultaneously returns to the Setup menu Press the left Softkey for BACK two times to get back to the main display 3 4 2 Sound The sounds for turning the adjustement wheel and for messages are set on by default Ihe user may choose to set them off or on To change the sound settings 1 Select MENU by pressing the left Softkey 2 Usethe adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select Sound Confirm the selection by pressing the operating button or the Softkey for OK 4 On the display the Adj Wheel setting is highlighted in On or Off mode a If you wish to change the setting turn the adjustment wheel Then press the operating button or the right Softkey for OK to confirm the change OR b Leave the parameter as it is by p
12. K Repeat for all numbers in your password 5 Next the pipette asks for a name for the memory location Activate the name editor by pressing the operating button B 6 With the middle Softkey S you can switch between characters numbers and symbols Turn the adjustment wheel A to change the characters and press the operating button B or the right Softkey R for OK to confirm the selection and to move to the next character If you want to cancel the character change you can press the left Softkey for BACK 7 Repeat the sequence in point 6 for all characters Confirm the last character with OK or the operating button and the pipette simultaneously exits the editing mode 8 If you want to clear any of the characters in the name turn the adjustment wheel until the character is underlined and press the right Softkey for CLEAR Repeat this until the desired characters are cleared 9 Save the program name by pressing the middle Softkey for SAVE S and the pipette returns to the list of memory locations 10 At any time if you wish to exit the current function and or do not want to save the latest change press the left Softkey for BACK d To activate the program from a memory location 1 Press the Hotkey H to see the list of memory locations 2 Choose the desired memory location by turning the adjustment wheel A 3 Press the operating button B or the right Softkey for OK to activate the saved program 4 The pipett
13. RY TO THE INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH TIPS OTHER THAN THE MANUFACTURER S ORIGINAL ONES Each Picus Picus NxT electronic pipette is tested before shipping by the manufacturer The Sartorius Quality Assurance Procedure guarantees that the Picus Picus NxT electronic pipette you have purchased is ready for use Each Picus Picus NxT electronic pipette is CE marked fulfilling the requirements of the EN 55014 1993 EN 55104 1995 ISO 13485 2003 and Directive 98 79 EC 6 Hazardous Substances RoHS II The product is in compliance with the European Union Directive RoHS Il 2011 65 EC on the restriction of use of hazardous substances The information is based on our supplier provided information Confirmation of compliance status by our suppliers is either the product does not contain any of the restricted substance s or the used concentrations are below the given threshold levels 7 Disposal WEEE In compliance with European Directive WEEE 2002 96EC on waste and reduction of hazardous substances in electrical and electronic equipments this device must not be disposed of as unsorted municipal waste Instead this device must be collected separately in accordance to the local recycling regulations Batteries should be disposed of in accordance with local legal regulations 8 Troubleshooting 8 1 Hardware Reset If the pipette is unresponsive it is possible to force turning off and resetting the hard
14. SH Sartorius Picus amp Picus NxT Electronic Pipette se User Manual t Sartorius E Table of Contents 1 Introduction 1 1 Intended use 1 2 Product Overview 1 2 1 Single and Multichannel Pipettes 1 2 2 Display 1 2 3 Softkey functions 1 2 4 Optifit Tips and Safetyspace Filter Tips 1 3 Content of delivery package 2 Getting Started 2 1 Charging 2 2 Power Up 3 Operation 3 1 Principles of Operation 3 2 Pipetting Modes 3 2 1 Pipetting Forward Pipetting 3 2 2 Reverse Pipetting 3 2 3 Multi Dispensing 3 2 4 Manual Pipetting 3 2 5 Diluting 3 2 6 Sequential Dispensing 3 2 7 Multi Aspirating 3 2 8 Titration 3 2 9 Additional Modes 3 3 Saving Pipetting Programs to Memory 3 4 Setup 3 4 1 Adjustment 3 4 2 Sound 3 4 3 Backlight 3 4 4 User ID 3 4 5 GLP Info 3 4 6 Reset 3 4 7 Information 3 4 8 Languages 3 4 9 Password 3 5 RFID 4 Care And Maintenance 4 1 Cleaning and Maintenance 4 1 1 Single Channel Pipettes 4 1 2 Multichannel Pipettes 4 2 Sterilizing 4 2 1 Autoclaving 4 2 2 UV Sterilization 4 2 3 Using Disinfectant Liquid 4 3 Performance Testing 4 4 Replacing the Battery 4 5 Replacement Parts 4 6 Storage 5 Warranty 6 Hazardous Substances RoHS II 7 Disposal WEEE 8 Troubleshooting 8 1 Hardware Reset 8 2 Troubleshooting Guide 9 Technical Data 9 1 Performance Specifications 9 2 Speed Table 10 Ordering Information 11 Declaration of Conformity CO
15. Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select GLP Info Confirm the selection by pressing the operating button or the Softkey for OK 4 The previously used dates are displayed on the screen Turn the adjustment wheel to edit the date month year Confirm the selection by pressing the operating button or the Softkey for OK and the next parameter becomes editable 5 Repeat the previous step for all parameters you want to change and confirm the last parameter with OK and simultaneously exit the GLP Info screen back to the Setup menu 6 You may exit at any time by pressing the left Softkey for BACK without saving any changes 7 Once you are back in the Setup menu press the left Softkey for BACK two more times to return to the main display 3 4 6 Reset The Reset function allows the user to return all factory settings and overwrite all personal settings including the stored programs To reset pipette settings treset all 1 Select MENU by pressing the left Softkey pipet settings 2 Use the adjustment wheel to select Setup Confirm the selection by to factory pressing the operating button or the Softkey for OK defaults 71 3 Use the adjustment wheel again to select Reset Confirm the selection by pressing the operating button or the Softkey for OK 4 he pipette asks if the user wants to reset all settin
16. ctronic pipettes USB charger universal included in the package Sartorius Charging Stand for one pipette order code 730981 Sartorius Charging Carousel for 4 pipettes order code 730991 The battery sign in the bottom right corner of the display indicates the battery s level of charge When the battery s level of charge is low the indicator blinks LOW and the pipette needs to be recharged NOTE Before connecting the AC charger to the charging stand and the mains electrical outlet make sure that power supply output voltage level and power capacity is correct Use of incorrect power supplies may damage the device Only use the power supplies recommended by the manufacturer 2 2 Power Up 1 Press ON OFF button Pipette will turn on 2 Press tip ejector button as advised on the display The pipette is now ready to be programmed and operated 3 While used and or charged pipette is in active mode all the processor functions are activated and the display backlight is on 4 1 minute after last activity The pipette will turn to a power saving mode and the backlight is dimmed The pipette returns to active mode when any of its buttons are pressed or the adjustment wheel is turned 5 10 minutes after last activity The backlight is turned off but the display is still visible The pipette returns to active mode when any of its buttons are pressed or the adjustment wheel is turned 6 60 minutes after last activity The pipette t
17. cy requirements of the application frequency of use number of operators using the pipette nature of the liquid to be dispensed and the acceptable maximum permissible errors established by the user ISO 8655 1 Performance tests should take place in a draught free room at 15 30 C kept constant within 0 5 C and relative humidity above 50 The pipette tips and the test water should have stood in the test room for long enough at least 2 hours to reach equilibrium with the room conditions Use distilled or de ionised water ISO 3696 grade 3 Use an analytical balance with a readability according to ISO 8655 6 Weighing 1 Adjust the desired test volume VS 2 Carefully fit the tip onto the tip cone 3 Fill the tip with test water and expel to waste five times to reach humidity equilibrium in the dead air volume 4 Replace the tip Pre wet the tip by filling it once with the test water and expell to waste 5 Aspirate the test water immersing the tip only 2 3 mm below the surface of the water Keep the pipette vertical 6 Withdraw the pipette vertically and touch the tip against the side wall of the test water container 7 Pipette the water into the weighting vessel touching the tip against the inside wall of the vessel just above the liquid surface at the angle of 30 to 45 Withdraw the pipette by drawing the tip 8 10 mm along the inner wall of the weighing vessel 8 Read the weight in mgs mi 9 Repeat the test cyc
18. e German Design Award Special Mention 2014 the reddot design award 2012 and the Fennia Price Honorary Mention 2012 These awards strongly reflect the user friendly and high quality design of the pipette Congratulations on becoming a new Picus Picus NxT owner German Design Award SPECIAL MENTION 2014 reddot design award winner 2012 fenniaprize 12 good design grows global HONORARY MENTION 1 1 Intended Use The Picus Picus NxT pipette is intended to be used in liquid handling applications for dispensing liquids in the volume range of 0 2 ul to 10 ml It is recommended to use Optifit Tips or SafetySpace Filter Tips to ensure the best compatibility and performance with Sartorius pipettes This liquid handling device is designed and manufactured to be used as general purpose laboratory equipment This manual contains useful information e g for good pipetting practice 1 2 Product Overview Before using the Sartorius Picus Picus NxT electronic pipette for the first time please review this operating manual carefully This manual can be downloaded from Sartorius website www sartorius com A paper print out may be ordered by e mail from Ihinfo finland Zsartorius com 1 2 1 Single and Multichannel pipettes Electronic tip ejector Charging contacts ON OFF button USB charging port Operating button amp volume range colour code Adjustment wheel Hotkey for memory places Softkeys for programmin
19. e is now ready for running the program The memory location is displayed next to the main mode name 3 4 Setup The user may change the settings of the pipette through the Setup menu The modifiable settings are the accuracy adjustment sound backlight user ID GLP info language password and reset to factory settings The following chapters introduce the various settings and give instructions on how to modify them 3 4 1 Adjustment The length of the piston stroke in Picus and Picus NxT pipettes is defined by the manufacturer as a factory setting This factory setting is to be used in normal circumstances with aqueous liquids at standard atmospheric pressure and with the pipette tip and the liquid at standard room temperature When these circumstances change the accuracy of the dispensed volume may be affected To maintain accuracy in altered circumstances the user can adjust the factory setting per the changed environmental factors or type of liquid to be used by using the Adjustment function L i lari H M10 indicates that the shown pipetting program has been saved In memory place 10 20 Adjustment is needed circumstantially to correct the dispensed volumes in the following cases when pipetting liquids with characterisitics that greatly differ from water for example viscous or volatile liquids when the temperature between the pipette pipette tip and liquid differs greatly when the ambient a
20. e middle Softkey for NEXT and move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 To exit the editing mode during programming press the left Softkey for BACK and the latest changes are not saved To execute the selected program 1 Press the operating button to aspirate the selected volume 2 Press the operating button again for pre out to make sure that the first aliquot will be of correct volume 3 Dispense the set aliquots by pressing the operating button for each aliquot 4 After the last aliquot has been dispensed press the operating button to empty the tip or press NO to start aspirating without emtying the remaining liquid 5 Eject the tip by pressing the electronic tip ejector button Additional modes Excess Adjustment and Repeated Blow out with Picus NxT can be used in conjunction with Sequential Dispensing Excess Adjustment can be used to set the excess volume Repeated Blow out helps to remove the residual liquid from the tip if the liquid is such that the normal dispensing does not completely empty the tip For more information on using additional modes see chapter 3 2 9 3 2 7 Multi Aspirating Multi Aspiration aspirates a selected volume a set number of times Multi Aspirating is a useful mode for example for sample pooling and microplate washing Select the volume and the number of aspirations desired Repeat the aspiration until the series i
21. each additional mode Tracker Tracker is a helpful feature for guiding the user to dispense into correct microplate wells It is a unique feature only available with Picus9 and Picus NxT pipettes and can be used with the following main modes Pipetting Reverse Pipetting and Multi Dispensing Once Tracker is activated continue choosing the related parameters for Single channel pipettes 1 Select the microplate size 96 or 384 well plate and the pipetting direction in rows or columns by scrolling the adjustment wheel 2 Then press the operating button or the right Softkey for OK to activate the Tracker The well into which to pipette is Shown on the display 4 If pipetting in rows A1 A2 A3 B1 B2 B3 C1 C2 C3 is displayed 5 f pipetting in columns 1A 1B 1C 2A 2B 2C 3A 3B 3C is displayed 6 Ihe first dispensing location can be selected as desired with the EDIT function of each main mode R 8 channel pipettes 1 Select the microplate size 96 or 384 well plate Pipetting is possible in columns only 2 Press the operating button or the Softkey for OK to activate the Tracker 3 he wells into which to pipette are shown on the display For 96 well plate columns 1 2 3 are shown For 384 well plate columns are showed as follows 1 pipetting A1 C1 ET 2 pipetting B1 D1 F1 3 pipetting A2 C2 E2 4 pipetting B2 D2 F2 and so on 4 The firs
22. ejector collar counter clockwise and remove it Hold the tip cone between your fingers open the bayonet lock by turning the bayonet locking ring clockwise until it stops and remove the tip cone Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with cleaning solution or similar and lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not overtighten Put a thin layer of autoclavable grease cat no 731141 on the seal and the interior of the tip cone avoid excess grease Carefully place the tip cone on the piston hold the tip cone and turn the bayonet locking ring counterclockwise until it stops to lock the tip cone Make sure that the tip cone Is properly tightened do not over tighten Attach the tip ejector collar by screwing it clockwise Insert the Safe Cone Filter with tweezers Turn the pipette on NOTE After cleaning or maintenance it is necessary to check the performance of the pipette 4 1 2 Multichannel Pipettes Cleaning the outer surface of all models Clean any visible dirt with cleaning solution or similar and a lint free cloth Wipe dry Cleaning and maintaining the lower parts of all models Opening the lower part of a multichannel pipette should only be done by an authorised Sartorius service provider Please contact your nearest Sartorius service
23. eously exit the editing mode OR b the middle Softkey for NEXT and move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 oexit the editing mode during programming press the left Softkey for BACK The latest changes are not saved To execute the selected program 1 Press the operating button to aspirate the liquid selected volume excess in 2 Press the operating button again to dispense the selected volume 3 To continue reverse pipetting without discarding the excess volume either a hold the operating button down and then release it for reaspiration OR b press the left Softkey for NO and start from the beginning 4 o discard the excess volume and to empty the tip press the operating button twice 5 Eject the tip by pressing the electronic tip ejector button Additional Modes Counter Excess Adjustment and Tracker can be used in conjunction with Reverse Pipetting Counter counts the number of dispensings Counting can be selected to start at any wanted number Excess adjustment can be used to set the excess volume When activated the excess volume is set to default Tracker advises the user in microplate dispensing by displaying the location for the next dispensing For more information on using additional modes see chapter 3 2 9 NOTE Only one additional mode can be selected at a time 3 2 3 Multi Dispensing Multi Dispensing aspirates t
24. ese and Russian available from the software version 1 07 onwards To change the language 1 Select MENU by pressing the left Softkey 2 Usethe adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select Languages Confirm the French German selection by pressing the operating button or the Softkey for OK 4 The list of languages are shown on the display a Use the adjustment wheel to select the desired language and press the operating button or the right Softkey for OK to activate the language and to return to the Setup menu OR b Press the left Softkey for BACK if you do not wish to change the language but to return to the Setup menu 5 Once you are back in the Setup menu press the left Softkey for BACK two times to return to the main display 3 4 9 Password The saved programs can optionally be protected with a password from Chinese Russian unauthorized editing in Picus NxT pipettes With the Password setting the password can be activated deactivated and changed To activate or deactivate the Password feature 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select Password Confirm the selection by pressing the operating button or the Softkey for OK 4 he prevailing
25. g Dot matrix display 0 Maximum volume 1 Dispensing head tip ejector collar and tip cone autoclavable excl 8 12 ch 1200 ul 12 Optiload spring loaded tip cones in multichannel pipettes 13 Safe Cone Filters excl 10 ul pipettes 14 Pipette tip d ue B9 cw OO qoe Je 1 2 2 Display The multi colour backlit dot matrix display is clear and the structure is informative Softkey functions Current mode Pipetting volume and aliquots in multidispensing modes Speed Battery charging indicator Arrows indicating aspiration and dispensing p UL oue c 1 2 3 Softkey Functions Left Softkey MENU BACK QUIT Middle Softkey EDIT NEXT SAVE uppercase characters lowercase characters digits symbols Right Softkey ADV OK CLEAR 1 2 4 Optifit Tips and SafetySpace Filter Tips It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes This ensures the best possible compatibility and thus best possible accuracy and precision as these tips are designed for the pipette in question Additional pipetting instructions to achieve good results are as follows The liquid and the pipette tip combination should be approximately at the same temperature Choose the right tip volume for your pipette the colour of the tip tray should match the colour code on the pipette Use Optifit Tips in rack refill or bulk packaging and choose the purity level your applicat
26. gs a To approve resetting press the right Softkey for YES or the operating button Follow the instructions on the screen and press the tip ejection button The settings are now reset to factory defaults OR b To cancel the resetting press the left Softkey for NO The settings are not reset and you are simultaneously returned to the Setup menu 5 Once you are back in the Setup menu press the left Softkey for BACK two times to return to the main display 3 4 7 Information The Information function displays the current sofware version of the pipette and the level of charge of the battery This information is not editable To view the information 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select Information Confirm the selection by pressing the operating button or the Softkey for OK 4 he display shows the current software version and the level of charge of the battery 5 To exit the screen press the right Softkey for OK or the operating button when you return back to the Setup menu 6 Once you are back in the Setup menu press the left Softkey for BACK two times to return to the main display 23 24 3 4 8 Languages The Languages setting provides the option to change the user interface language The available languages are English French German Chin
27. he selection by pressing the right Softkey for OK or the operating button 8 he next character to be edited is highlighted Repeat steps 6 and 7 to edit all characters a When you have edited and accepted all characters press the middle Softkey for SAVE OR b If you do not want to save the edited ID but to keep the former one then press the left Sofkey for BACK 9 Once you are back in the Setup menu press the left Softkey for BACK two more times to return to the main display To delete the characters 1 Follow steps 1 to 4 above to see the current ID in use 2 Turn the adjustment wheel to underline the character to be deleted and press the right Softkey for CLEAR Repeat step 2 for every character to be deleted 4 When you have deleted all the characters press the middle Softkey for SAVE and you will be returned to the Setup menu or continue editing the ID by pressing operation button Please note that all characters cannot be deleted 5 f you do not want to save the edited ID but to keep the former one then press the left Sofkey for BACK 6 Once you are back in the Setup menu press the left Softkey for BACK two more times to return to the main display v 3 4 5 GLP Info GLP Good Laboratory Practice Info feature allows the storage of dates to indicate when the pipette was last serviced calibrated and when the next service calibration is due To change the dates 1 Select MENU by pressing the left
28. he total volume as well as an excess volume then repetitively dispenses equal volumes of liquid Recommended in long pipetting series and microplate dispensing To select Multi Dispensing mode 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Multi Disp Confirm the setting by pressing the operating button or the Softkey for OK When Multi Dispensing mode is selected the latest settings used will be displayed To edit the parameters volume number of dispensings speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highlighted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK when you simultaneously exit the editing mode OR b the middle Softkey for NEXT when you can move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 To exit the editing mode during programming press the left Softkey for BACK The latest changes are not saved To execute the selected program 1 Press the operating button to aspirate the liquid selected volume excess in 2 Press the operating button again to discard the prime excess 3 Press the operating button repeatedly untill all aliquots have been dispensed 4 o continue repetitive dispensing without discarding the excess volume
29. ion requires free of DNase RNase and endotoxin and or pre sterilized n case you need to be aware of aerosol contamination you may choose between Safe Cone Filters which are attached to the tip cone and should be changed daily or SafetySpace Filter Tips which are discarded after each pipetting Choose SafetySpace Filter Tips also when you want to avoid loss of sample due to the sample reaching the filter The extra space between the sample and the filter ensures that any sample neither foaming nor viscous liquids reach the filter when in the reverse pipetting or repetitive multiple dispensing mode of the electronic pipette Pre rinse the tip 3 5 times before pipetting especially important in forward pipetting mode After dispensing wipe the tip against the receiving vessel s wall to catch the last droplet Change the tip after every pipetting 1 2 3 4 5 6 SafetySpace air gap i F E ore x 1 3 Content of Delivery Package Picus Picus NxT is delivered ready for use in a package containing Picus Picus NxT electronic pipette USB charger Sartorius tip rack 10x96 tips with up to 1000 ul single channel models 1200 ul multichannel models One tip with 5 ml and 10 ml single channel models Safe Cone Filters and tweezers with models gt 10 ul Autoclavable grease with single channel models QC certificate Picus9 certificate of accredited 3 point calibration Picus N
30. ipette s lifetime Sartorius Safe Cone Filters available for pipettes gt 10 ul or Sartorius SafetySpace Filter Tips should be used Sartorius provides service maintenance and calibration services through its global network of service centres Please contact your nearest service centre for assistance NOTE Please note that the warranty will be voided in the event that the pipette is disassembled or assembled by an unauthorised person 4 1 Cleaning and Maintenance Sartorius electronic pipettes can be cleaned and decontaminated by using disinfectant or decontaminant liquids such as 70 ethanol 60 isopropanol mild detergent or similar Always check the chemical compatibility between the materials of the pipette and the disinfectant or decontaminant liquid Turn off the pipette prior to cleaning t is recommended to always use gloves when cleaning the pipette Change the Safe Cone Filter regularly with the tweezers provided with the pipette Cleaning is always recommended after possible contamination or dispensing corrosive or other aggressive chemicals Never let liquids enter the inner parts of the pipette 25 Never use the pipette without tip attached to the tip cone Tools needed lint free cloth cleaning solution e g 70 ethanol 4 1 1 Single channel pipettes Cleaning the outer surface of all models Clean any visible dirt with mild detergent or similar and a lint free cloth Wipe dry M
31. ir pressure differs from that of standard atmospheric pressure NOTE When the factory settings are replaced with adjusted settings the performance specifications random amp systematic error of the pipette given by the manufacturer are no longer applicable However when the factory settings are reactivated the performance specifications will apply again The Adjustment function enables the user to adjust the pipette in 1 2 or 3 calibration points The more measurement points are selected the better accuracy in results is reached over the whole volume range of the pipette Single point adjustment is recommended when pipetting a constant volume within a range and 2 or 3 point adjustment if performance over the whole range is required The adjustment options are 1 point adjustment adjustment in one point only the user can choose the desired adjustment point volume 2 point adjustment adjustment in 10 9o and 100 of the nominal volume 3 point adjustment adjustment in 10 50 9o and 100 of the nominal volume When adjusting for accuracy the actual volumes delivered should be measured in Pipetting mode Once the adjustment is activated it is in force for all pipetting modes of the pipette and an ADJ symbol is displayed on the screen It is possible to store three 3 adjustments in the adjustment memory ADJ1 ADJ2 ADJ3 These can be activated at any time for cases of specific use that require accuracy adjustmen
32. l 300 ul 1000 ul 5 ml 10 ml 1 Z5 6 0 7 7 10 1 10 2 10 2 2 1 8 4 2 53 7 4 7 4 7 4 3 1 3 2 9 3 7 5 4 5 4 5 4 4 1 0 2 1 2 7 3 8 3 8 3 8 5 0 8 1 5 1 9 2 8 2 7 Za 6 0 6 1 1 1 4 19 1 8 2 2 7 0 5 0 9 1 1 1 2 1 1 1 7 8 0 4 0 7 0 9 0 8 0 8 13 9 0 3 0 6 0 8 0 6 0 6 0 9 Multichannel pipettes speed in seconds Speed 10 ul 120 ul 300 ul 1200 ul 1 2 5 6 1 5 4 6 1 2 1 8 4 4 3 9 4 4 3 13 3 2 9 5 4 1 0 2 4 2 1 2 5 5 0 8 1 8 1 6 1 9 6 0 6 1 4 1 2 1 4 7 0 5 1 1 1 0 11 8 0 4 0 9 0 8 0 9 9 0 3 0 7 0 7 0 7 10 Ordering Information Picus NxT and Picus Picus NxT Order Code LH 745021 LH 745041 LH 745061 LH 745081 LH 745101 LH 745111 LH 745321 LH 745341 LH 745361 LH 745391 LH 745421 LH 745441 LH 745461 LH 745491 Picus Order Code 735021 735041 735061 735081 735101 735111 735321 735341 735361 735391 735421 735441 735461 735491 Channels Volume Range pl Safe Cone Filters 12 12 12 12 0 2 10 95120 10 300 50 1000 100 5000 500 10000 0 2 10 5 120 10 300 50 1200 0 2 10 5 120 10 300 50 1200 Low Retention Tips are available in volumes up to 1200 ul All pipettes include universal charger EU UK US JPN AUS and CHN plugs Standard 721008 721007 721006 721005 721005 721008 721007 721006 721008 721007 721006 Plus 721018 721017 721016 721015 721015 721018 721017 721016 721018 721017 721016 Optifit Tips ul SafetySpace Filter Ti
33. le until 10 measurements have been recorded 10 Convert the recorded masses mi to volumes vi by multiplying the mass with the correction factor Z Z values in the table below Vi 2 mi Z Z values ul mg Temp C X Air Pressure kPa 95 100 101 3 105 20 0 1 0028 1 0028 1 0029 1 0029 20 5 1 0029 1 0029 1 0030 1 0030 21 0 1 0030 1 0031 1 0031 1 0031 Zka 1 0031 1 0032 1 0032 1 0032 22 0 1 0032 1 0033 1 0033 1 0033 22 5 1 0033 1 0034 1 0034 1 0034 23 0 1 0034 1 0035 1 0035 1 0036 23 5 1 0036 1 0036 1 0036 1 0037 12 Calculate the mean volume V delivered V Vi 10 13 For conformity evaluation calculate the systematic error eS of the measurement in ul eS V VS VS selected test volume or in eS 100 V VS VS 14 For conformity evaluation calculate the random error of the measurement as standard deviation z Ii W n number of measurement 10 V n 1 or as coefficient of variation CV 100s V 15 Compare the systematic error inaccuracy and random error imprecision to the performance specification values of your own laboratory NOTE Systematic error is the difference between the dispenced volume and the selected test volume Random error is the scatter of the dispensed volumes around the mean of the dispensed volume ISO 8655 1 NOTE Sartorius specifications are achieved in strictly controlled conditions ISO 8655 1 Users should establish acceptable maximum permissible errors based on the field of use
34. n Put a thin layer of autoclavable grease cat no 731141 around the seal Carefully place the tip cone on the piston attach it by screwing the tip cone locking ring clockwise Attach the tip ejector collar by screwing it clockwise Insert the Safe Cone Filter with tweezers Turn the pipette on Model 5 ml cleaning and maintaining the lower parts Turn off the pipette Remove the Safe Cone Filter if attached Unscrew the tip ejector collar counter clockwise and remove it Hold the tip cone holder between your fingers unscrew the tip cone by turning it counter clockwise and then remove it Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with mild detergent or similar and a lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not over tighten Put a thin layer of autoclavable grease cat no 731141 on the seal and the interior of the tip cone avoid excess grease Carefully place the tip cone onto the piston hold the tip cone holder and screw it in by turning it clockwise Attach the tip ejector collar by screwing it clockwise Insert the Safe Cone Filter with tweezers Turn on the pipette 26 Model 10 ml cleaning and maintaining the lower parts Turn off the pipette Remove the Safe Cone Filter if attached Unscrew the tip
35. n be calibrated then the display will show Calibration values out of range 9 Repeat steps 7 and 8 for 2 or 3 point adjustment until all volumes are set 10 50 100 Pipette without adjustment Target Volume 10 50 100 1 point adjustment in Picus Target Volume 10 50 100 A 2 point Adjustment in Picus Target Volume P ad a 7 d e K 10 96 50 96 100 96 83 point Adjustment in Picus Target Volume Theoretical comparison of target and actual values without adjustment In this 1 point adjustment it has been opted to use 50 of maximum volume to match actual and target results In 2 point adjustment 10 and 100 of maximum volume are used to match actual and target results In 3 point adjustment 10 50 and 100 of maximum volume are used to match actual and target results 10 Then the pipette asks if you want to save the adjustment data Confirm the saving of the data by pressing the operating button or the right Softkey for YES If you do not wish to save the data press the left Softkey for NO 11 Note that you may exit the editing mode without saving any changes at any time by pressing the left Softkey for BACK To activate the saved adjustment settings 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3
36. odels 10 pl and 120 ul cleaning and maintaining the lower parts Turn off the pipette Remove the Safe Cone Filter if attached Unscrew the tip ejector collar counter clockwise and remove it Unscrew the tip cone locking ring counter clockwise and carefully remove it Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with cleaning solution or similar and a lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not over tighten Put a thin layer of autoclavable grease cat no 731141 on the piston Carefully replace the tip cone and attach it by turning the tip cone locking ring clockwise Insert a Safe Cone Filter with tweezers Safe Cone Filter in models gt 10 ul Turn the pipette on Models 300 ul and 1000 ul cleaning and maintaining the lower parts Turn off the pipette Remove the Safe Cone Filter if attached Unscrew the tip ejector collar counter clockwise and remove it Unscrew the tip cone locking ring counter clockwise and carefully remove it Unscrew the exposed piston counter clockwise Clean the tip ejector collar tip cone locking ring tip cone and piston with cleaning solution or similar and a lint free cloth Rinse with distilled water if needed and let the parts dry Install the piston by screwing it clockwise do not over tighte
37. oes not completely empty the tip For more information on using additional modes see chapter 3 2 9 3 2 8 Titration In Titration mode the full volume is aspirated after which dispensing speed is manually controlled The display shows the dispensed volume in real time during dispensing Titration is used to determine the unknown concentration of an identified analyte To select Titration mode 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Titration Confirm the setting by pressing the operating button or the Softkey for OK The Titration mode is activated with the latest settings To edit the parameters volume speed volume for Fast Dispensing in case it is enabled 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the left or right The first parameter to be edited is now highlighted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultaneously exit the editing mode OR b the middle Softkey for NEXT and move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 oexit the editing mode during programming press the left Softkey for BACK and the latest changes are not saved To execute the selected program 1 Press the operating button to aspirate the set volume 2 Press again the operating button and h
38. old it down or turn the adjustment wheel to the left to dispense the liquid You may influence the dispensing speed by how far left you turn the adjustment wheel You may pause the dispensing by releasing the operating button or the adjustment wheel for a while 3 To finish titrating empty the tip by pressing the operating button twice 4 When the titration cycle is completed eject the tip by pressing the electronic tip ejector button Additional modes Fast Dispensing can be used with the Titration mode Fast Dispensing dispenses the first volume automatically After dispensing the first volume dispensing is continued manually For more information on using additional modes see chapter 3 2 9 3 2 9 Additional Modes The additional modes can be optionally used in conjunction with the main pipetting modes as shown on the table on page 9 of this manual To activate or deactivate the additional modes once you have already selected the main mode 1 Choose ADV by pressing the right Softkey All available additional modes are listed and their current status On Off is displayed 2 Scroll through the list of additional modes using the adjustment wheel 3 Select the desired highlighted additional mode by pressing the right Softkey for OK or the operating button The mode is changed On or Off 4 If On is selected continue with other parameter selections where relevant for the additional mode in question as instructed below for
39. onfirm the selection by pressing the operating button or the Softkey for OK 4 All colour options are listed Choose the desired colour by using the adjustment wheel a Confirm the selection by pressing the operating button or the Softkey for OK OR b Press the left Softkey for BACK to exit the list without saving any changes 5 Once you are back in the Setup menu press the left Softkey for BACK two times to return to the main display 3 4 4 User ID The pipette can be named to help identify it The user can for example add her his name to the pipette so that laboratory technicians can know which pipette belongs to whom When the pipette is turned on the name is displayed on the screen To change the ID name 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select User ID Confirm the selection by pressing the operating button or the Softkey for OK 4 he previously used name is displayed on the screen 5 To edit the name press the operating button when the first chracter becomes highlighted and can be edited 6 Capital letters are used as default Use the middle Softkey to change the character type ABC indicates capital letters abc lower case letters 123 numbers and other characters 7 Turn the adjustment wheel to select the character you desire and confirm t
40. ps pl 10 200 350 350 1000 5000 10000 10 200 350 350 1200 10 200 350 350 1200 10 120 300 1000 10 120 300 1200 10 120 300 1200 35 36 11 Declaration of Conformity 38 sartorius C EC Certificate of Conformity the undersigned hereby declare that the Product s Picus amp Picus NxT Electronic Pipettes Charging Stand and Charging Carousel conforms to the protection requirements of Council Directive 2004 108 EC relating to Electromagnetic Compatibility EN61326 and Safety IEC 61010 1 ed 3 0 by the application of Technical Construction File No 01 12 dated 26 04 2012 and Competent Body Technical Report Certificate No 198349 dated 25 04 2012 and Report No 198349E dated 31 05 2012 issued by Nemko Oy Perkkaantie 11 FIN 02601 Espoo Finland Tel 358 424 545 41 his declaration is supported by EC quality system approval certificates ISO 9001 ISO 13485 Certificate No 108129 201 1 AQ FIN FINAS issued by DNV on 20 December 2011 ISO 17025 Certificate No K041 issued by FINAS on 16 December 2011 ISO 14001 Certificate No 108133 2011 AE HEL FINAS issued by DNV on 20 December 2011 a yi E ay Signed Date 3 June 2014 Full Name G Heini Title CEO Company Sartorius Biohit Liquid Handling Oy Laippatie 1 00880 Helsinki Finland Sartorius Biohit Liquid Handling Oy Head Office Laippatie 1 00880 HELSINKI FINLAND Tel 358 9 755 951 Fax 358
41. pty the tip For more information on using additional modes see chapter 3 2 9 3 2 5 Diluting In Dilution mode liquids separated by an air gap are aspirated and then dispensed simultaneously Diluting can be used for diluting samples and reagents Aspirate first the diluent followed by an air gap and finally aspirate the sample or reagent to avoid contamination of the diluent To select Diluting mode 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Diluting Confirm the setting by pressing the operating button or the Softkey for OK To edit the parameters volumes speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highlighted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultaneously exit the editing mode OR b the middle Softkey for NEXT and move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 oexit the editing mode during programming press the left Softkey for BACK and the latest changes are not saved To execute the selected program Press the operating button to aspirate the dilutor Press the operating button again to aspirate the air gap Press the operating button the third time to aspirate the sample Dispense the entire volume by
42. r 2x20W twin tubes Distance from tube surface to table product surface 620 mm Tested UV dosage 33000 mJ cm 4 2 3 Using Disinfectant Liquid Sartorius electronic pipettes can be cleaned and decontaminated using disinfectant or decontaminant liquids such as 70 ethanol 60 isopropanol mild detergent or similar Always make sure of the chemical compatibility of the materials of the pipette and the disinfectant or decontaminant liquid Single channel pipettes Wipe the outer surface of the single channel pipettes with a decontaminant fluid 70 ethanol 60 isopropanol mild detergent or similar For complete decontamination of the lower parts tip ejector collar tip cone spring and piston they can be placed into a dish containing disinfectant or decontaminant liquid Leave the parts to soak for 30 minutes Rinse the parts with distilled water and leave to dry before reassembling Multichannel pipettes Wipe the outer surface of the multichannel pipettes with a decontaminant fluid 70 ethanol 60 isopropanol mild detergent or similar The lower part of a multichannel pipette should be opened only by an authorised Sartorius service provider 4 3 Performance Testing It is recommended that the performance of the Sartorius pipettes is checked regularly e g every 3 months and always after in house maintenance A regular testing routine should be established by users for their pipettes taking into consideration the accura
43. ressing the operating button or the right Softkey for OK to accept the setting 5 The Messages parameter becomes highlighted in On or Off mode Repeat steps 4 a or 4 b 6 Atany time you may press the left Softkey for BACK to exit the editing mode without saving any changes 7 Once you are back in the Setup menu press the left Softkey for BACK two times to return to the main display 3 4 3 Backlight The backlight colour of the display can be changed By default it is white gray while the pipette is in use but while it is charging the colour changes to match the volume colour code The operating button and matching tips are similarly colour coded to aid tip selection If the backlight colour is changed by the user the selected colour is applied both while the pipette is in use and while it is charging Choose the desired target volume Insert the volume actually measured To save the adjustment press the Softkey for YES or the operating button The difference between the target volume and the actual value exceeds the limits that can be calibrated The pipette is set to use the adjustment values saved to memory place ADJ1 21 22 To change the colour of the display backlight 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Use the adjustment wheel again to select Backlight C
44. rgers charging stands USB charger for Picus9 Picus9 NxT pipette Input voltage Output voltage Output connector type AC charger for charging stand for 1 pipette Input voltage Output voltage AC charger for charging stand for 4 pipettes Input voltage Output voltage Picus Picus NxT electronic pipette Operating temperature Air humidity Weight Single channel 10 300 ul Single channel 50 1000 ul 8 channel 10 300 ul Length Single channel 10 300 ul Single channel 50 1000 ul 8 channel 10 300 ul Pipetting force for single and multichannel models Tip ejecting force for single and multichannel models Li Polymer battery with protection circuit 3 7 V 350 mAh Approx 1 hour For Indoor and Office Equipment Use Only 100 240V 50 60Hz 180mA 5V 1A LPS Micro USB According to local requirements 7 5 VDC 300 mA According to local requirements 9 VDC 1200 mA 15 C to 40 C Max 80 100 g 110 g 160 g 21 0 cm 21 6 cm 21 6 cm 1 3 N 3 1 N 9 1 Performance Specifications 1 channel 0 2 10 ul 5 120 ul 10 300 ul 50 1000 pl 0 1 5 ml 0 5 10 ml 8 channel 0 2 10 ul 5 120 ul 10 300 yl 50 1200 ul 12 channel 0 2 10 ul 5 120 ul 10 300 ul 50 1200 ul Increment Test volume ul 0 01 0 10 0 20 1 00 5 00 10 00 0 01 0 10 0 20 1 00 0 01 0 10 0 20 1 00 ul 10 5 1 120 60 12 300 150 30 1000 500 100 5000 2500 500 10000
45. s completed then discard the full aspirated volume in a single step To select Multi Aspiration mode 1 Select MENU by pressing the left Softkey 2 Usethe adjustment wheel to select Multi Aspiration mode Confirm the setting by pressing the operating button or the Softkey for OK When Multi Aspiration mode is selected the latest settings used will be displayed To edit the parameters volumes number of aspirations speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highlighted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultaneously exit the editing mode OR b the middle Softkey for NEXT and move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 oexit the editing mode during programming press the left Softkey for BACK and the latest changes are not saved To execute the selected program 1 Press the operating button repeatedly until all the set volumes are aspirated 2 To dispense the liquid and empty the tip press the operating button again 3 Eject the tip by pressing the electronic tip ejector button Additional mode Repeated Blow out available with Picus9 NxT can be used in conjunction with Multi Aspiration mode if the liquid is such that the normal dispensing d
46. ssing the left Softkey 2 Confirm Pipetting mode by pressing the operating button or the right Softkey for OK Once Pipetting is selected the latest settings used will be displayed To edit the parameters volume speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highlighted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultaneously exit the editing mode OR b the middle Softkey for NEXT and you move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all the parameters you wish to edit 5 oexit the editing mode during programming press the left Softkey for BACK and the latest changes are not saved To execute the selected program 1 Press the operating button to aspirate the liquid in 2 Press the operating button again to dispense the liquid 3 Eject the tip by pressing the electronic tip ejector button Additional modes Counter Mixing and Tracker can be used in conjunction with the Pipetting mode Additionally in Picus NxT Repeated Blow out is available for use with the Pipetting mode Repeated Blow out helps to remove the residual liquid from the tip if the liquid is such that normal dispensing does not completely empty the tip Counter counts the number of dispensings Counting can be selected to star
47. ssing the operating button or the right Softkey for OK Repeated Blow out Repeated Blowout allows the user to activate and repeat the blowout of the remaining liquid droplets when dispensing liquids that easily leave a residue in the tip Repeated Blowout is available with Pipetting Manual Pipetting Diluting and Multi Aspiration modes To activate deactivate Blowout 1 Start the main mode in question per the instructions given earlier 2 Once the pipetting cycle has ended you may start the blowout by pressing the operating button as many times as you see needed 3 To exit Blowout turn the adjustment wheel or eject the tip by pressing the tip ejector button To exit Blowout turn the adjustment wheel or eject the tip by pressing the tip ejector button 3 3 Saving Pipetting Programs to Memory All Picus and Picus NxT models allow the saving of 10 programs into the memory of the pipette To store the program in the memory 1 Choose the pipetting mode and set the parameters as guided in the previous chapter then press the Hotkey H to see the list of memory locations 2 Choose the desired memory location by turning the adjustment wheel A Press the middle Softkey for SAVE S 4 The pipette asks for a password if the password feature has been activated Enter the password one number at a time by turning the adjustment wheel and confirming the number by pressing the operating button or the right Softkey for O
48. status of the Password feature is highlighted on the display as either On or Off 5 Change the status of the Password On Off by pressing the operating button or the Softkey for OK 6 Ihe display asks you to enter the current password a Enter the password one number at the time by using the adjustment wheel and confirm the number by pressing the right Softkey for OK or the operating button Repeat this until you have inserted all numbers OR b Press the left Softkey for BACK at any time to exit the screen without changing the status 7 Once the last number is confirmed the status of the password is changed On Off and is displayed on the screen 8 Exit the Password display by pressing the left Softkey for BACK 9 Once you are back in the Setup menu press the left Softkey for BACK two more times to return to the main display To change the password 1 Repeat steps 1 3 above to access the Password display 2 Turn the adjustment wheel to highlight Set new password 3 Press the right Sofkey for OK or the operating button to edit the password 4 The display asks you to enter the current password a Enter the password one number at the time by using the adjustment wheel and confirm the number by pressing the right Softkey for OK or the operating button Repeat this until you have inserted all 4 numbers OR b Press the left Softkey for BACK at any time to exit the screen without saving any changes 5 The displa
49. t dispensing column can be selected as desired with the EDIT function of each main mode 1 F 1 4 3 ih i 7 i LETT UN iz e Oo O0 6 OD Oo O0 C amp e e c ec c Uc ooo r rommooososs som o o o y o o o e 0 woo o o a 0 0 s D d D O 02 OO D oo O0 OO 12 channel pipettes 1 Select the microplate size 96 or 384 well plate Pipetting is possible in rows only 2 Press the operating button or the right Softkey for OK to activate the Tracker 3 The wells into which to pipette are shown on the display For 96 well plates rows are shown as A B C For 384 well plate rows are shown as follows 1 pipetting A1 A3 A5 2 pipetting A2 A4 A6 3 pipetting B1 B3 B5 4 pipetting B2 B4 B6 and so on 4 The first dispensing column can be selected as desired with the EDIT function of the main mode Counter Counter counts the number of pipetting cycles up to 384 and can be used in conjunction with Pipetting and Reverse Pipetting modes You may start counting from any desired number lt 384 The starting number can be set with the adjustment wheel right after the additional mode has been activated or with the EDIT function of the main mode Mixing With the Mixing mode the pipette mixes the liquids either when the operating button is held down manually or automatically a given number of times The Mixing mode can be used in conjunction with Pipetting and
50. t in any desired number Mixing mixes the liquids manually or automatically The mixing volume can be adjusted according to the pipette s maximum volume Tracker advises user in microplate dispensing by displaying the location for the next dispensing For more information on using additional modes see chapter 3 2 9 NOTE Only one additional mode can be selected at a time Repeated Blow out can be combined with other additional modes 3 2 2 Reverse Pipetting Reverse Pipetting aspirates the selected volume as well as an excess volume Recommended for biological foaming and viscous liquids In Reverse Pipetting mode the excess is left inside the tip and is then discarded To Select Reverse Pipetting mode 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Reverse Confirm the setting by pressing the operating button or the Softkey for OK Once Reverse Pipetting mode is selected the latest settings used will be displayed To edit the parameters volume speed 1 Press the middle Softkey for EDIT or turn the adjustment wheel all the way to the right The first parameter to be edited is now highlighted MENU Press the Softkey for MENU Choose the mode Press the Softkey for EDIT NEAI UK Change settings and press OK or NEXT 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultan
51. ted 2 Turn the adjustment wheel to set the desired value 3 Confirm the setting by pressing a the operating button or the right Softkey for OK and simultaneously exit the editing mode OR b the middle Softkey for NEXT and move on to edit the next highlighted parameter 4 Repeat steps 2 amp 3 for all parameters you wish to edit 5 oexit the editing mode during programming press the left Softkey for BACK and the latest changes are not saved To execute the selected program 1 Press the operating button to activate the aspiration of the liquid 2 Press the operating button again and hold it down or turn the adjustment wheel to the right to aspirate the liquid You may influence the aspiration speed by how far right you turn the adjustment wheel You may pause the aspiration by releasing the operating button or the adjustment wheel for a while 3 To activate the dispensing of the liquid turn the adjustment wheel to left Keep the adjustment wheel to left or hold the operating button down as long as you want to continue dispensing 4 When the entire volume Is dispensed press the operating button to empty the tip OR select NO left Softkey to continue directly with aspiration 5 Eject the tip by pressing the electronic tip ejector button Additional modes Repeated Blow out available with Picus NxT can be used in conjunction with Manual Pipetting mode if the liquid is such that normal dispensing does not completely em
52. ts of the pipette To change the accuracy adjustment settings 1 Select MENU by pressing the left Softkey 2 Use the adjustment wheel to select Setup Confirm the selection by pressing the operating button or the Softkey for OK 3 Adjustment function is highlighted Confirm the selection by pressing the operating button or the Softkey for OK 4 Use the adjustment wheel to select ADJ1 ADJ2 or ADJ3 memory location Edit the adjustment values by pressing the middle Softkey for EDIT 5 Use the adjustment wheel to select 1 Point 2 Points or 3 Points per your need Confirm the selection by pressing the operating button or the Softkey for OK 6 If you chose 1 point adjustment you next choose the desired target volume for which you desire the result accuracy to be adjusted by turning the adjustment wheel Confirm the volume selection by pressing the operating button or the Softkey for OK 7 f you chose 2 or 3 point adjustment the target volumes are already set Just confirm them by pressing the operating button or the Softkey for OK 8 Once you have set or accepted the target volume the actual volume is required to be inserted Now you insert the actual currently delivered volume per your measurement result Turn the adjustment wheel to choose the actual volume Confirm the selection by pressing the operating button or the Softkey for OK If the difference between the target volume and the actual value exceeds the limits that ca
53. urns OFF To power up the pipette again press operating button or ON OFF button The pipette will turn on also when charged If the pipette is turned off by pressing ON OFF button it can be turned on only by pressing the same button Hira 3 Operation 3 1 Principles of Operation Pipetting functions are controlled using the operating button adjustment wheel and Softkeys The Hotkey Is for storing or activating a program Operating button Used to confirm the settings alternative to the Softkey for OK and initiate the piston movement for aspiration dispensing and repeated blow out Adjustment wheel for fast volume setting Used for scrolling the menu and for volume settings Moves the piston in manual and titrating modes for aspiration and dispensing Unlocks the edit menu turn the adjustment wheel to the end to unlock the pipette for editing in active mode Electronic tip ejector Ejects the tip s with a light touch Left Softkey MENU Displays the main mode selection BACK Exits the MENU EDIT or ADV functions or the current display without saving the latest change setting QUIT Quits the pipetting task Middle Softkey EDIT Activates the editing mode in order to change parameters NEXT Active in editing mode Confirms the parameter setting and moves to the next parameter to be edited SAVE Active in memory settings Saves the selected program to the memory location
54. ware This function will not affect the saved settings e g adjustment memory To turn off an unresponsive pipette press simultaneously ON OFF button and the right Softkey for a few seconds Pipette will turn off and can then be turned on again by pressing ON OFF button NOTE Make sure the pipette is not placed on the charging unit or connected to the USB cable while being reset 8 2 Troubleshooting Guide Problem Possible Cause Solution Leakage Incompatible tip Use original Sartorius tips Tip is loose Attach the tip firmly Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service Inaccuracy Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service Pipette does not turnon Pipette is in OFF mode Turn the pipette on by pressing ON OFF button Empty battery Connect the pipette to the charger Piston is jammed Inner lower parts are loose Open the lower part and tighten the parts Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service Pipette does not aspirate Safe Cone Filter is dirty Replace the Safe Cone Filter liquid properly Pipette is dirty Clean the pipette Pipette is broken Replace the broken parts or send the pipette for service 31 gt 9 Technical Data Rechargeable battery Type Capacity Charging time Cha
55. xT Quick Start Guide Tip information card If any item in this delivery package is missing or damaged please contact your local Sartorius representative Bi pe i 2 Getting Started Please read this manual before using Picus Picus NxT electronic pipette 1 Picus Picus NxT is delivered ready for use 2 Insert a replaceable Safe Cone Filter into the tip cone before use to prevent contamination of the pipette 3 Charge the pipette before first use for one 1 hour 4 Press the ON OFF button on the top of the pipette to turn the power on 5 Press tip ejector button when advised by the display 6 The pipette is now ready to be programmed and operated 2 1 Charging It is recommended that you charge the pipette before first use for one 1 hour Place the pipette onto the charging stand or connect to the USB charger Make sure that the charging stand is connected to the power outlet and that the charging contacts of the pipette are properly in contact with the charging grooves of the charging stand Charging with USB charger Connect the USB cable to the pipette and plug the charger into the mains power Charging in Charging Stand or Carousel Make sure that the charging stand is connected via the AC charger to the mains power outlet and that the charging contacts of the pipette are inserted in the charging grooves of the charging stand making proper contact Charging options for Sartorius ele
56. y asks you to enter the new password a Enter the password one number at the time by using the adjustment wheel and by accepting the number by pressing the right Softkey for OK or the operating button Repeat this until you have Inserted all 4 numbers OR b Press the left Softkey for BACK at any time to exit the screen without saving any changes 6 When you have accepted the last number the password has been changed and New password set text is displayed on the screen after which you are returned to the Password display 7 Exit the Password display by pressing the left Softkey for BACK 8 Once you are back in the Setup menu press the left Softkey for BACK two times to return to the main display 3 5 RFID Picus9 NxT is RFID enabled to support asset management To recall the information stored on the RFID chip the reader and the software for RFID type HTS2048 are required On delivery the RFID chip in the Picus NxT contains the following information Product code Serial number Calibration certificate number Calibration date 4 Care and Maintenance Any precision instrument such as a pipette comprises of a great variety of mechanical and electronic parts that are subject to wear and tear By taking care of the pipette and carrying out regular maintenance and calibration the functionality and performance of the pipette can be ensured To avoid contamination of the pipette and the sample and to extend the p
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