Home

PAL User Manual Installation and Operation COMBI PAL / PAL LHX

1. sens su Figure 56 COMBI PAL Firmware Overview Page 3 syste PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt User Manual PA system Installation and Operation D IquioJaAQuu Tyd ON Ped Sme Apeas Oy Apeay wos awn apk 35 uado Surueap 304 pasn aq ji uorjes Suruonipuo Jaqi 20 lt SI auum ji 0 Suiueap Jaqi JOJ IUN J8800 SU LILI uorqiosap PLAY UI G 0 Z 1opelui jo ad uo spuadag WLU Gp 0 GC 1 fuy 99 uoqquospe qe Juawdojanap poujaui juapuadap uONenjddy uado ____ ABurpionne jew ur auinjon ajdwessnipy wis ic ZZ au peixa pue ssa301d uoneqrout sid 10 aum YO sc aum penx puesseouduogeqmuraidjojaugu sG juo ssaxo1d uoneqrouraid 104 paads uidi 054 01 0S7 juapuadap Jaqy pue uoreorjddy Jo 007 01 SE auum 1noayeg JaqiJ awr quossq payasur si Jaqy ajojeq sjduues ayesquinba 0 aum uoneqmouralg Sajnului BISNIS 10 Q SJajatuPJed POULIN Tvd syeway papuawiworay Jopalul joy ul snoauejuods spoe Juego jo uoneoiuams3 aduiex3 uonnjos ajduues auo WO jou Buus au UI pappe aq JW piepue s jeuraju uoners USeM WO pepuejs uorisog 83 uonnjos yuaBeay JO pjepuejs jPuJajuj JOY 24 UNOS PA UOI ElS USPM wos USEM uonsog d a el a2unoS PA a8uu s piepuejs Tri OL e uim Tr g i gt sauinjoA ajdwes palur o pasn Aureyy 0 0 SOZAS sw g su Q Su si oz 5 sj oc 0 Lu 35 I T
2. o32 Figure 25 Demonstrating a possible Inclination of Tray in X Y Z Axes 4 2 2 1 Definition of a Tray Row and Column The PAL System defines Rows and Columns by the order in which samples are treated A Row is not associated with an X or Y axis The definition Is visualized below with Tray Type VT32 and DW96 u eu el v y up up 12 345 8 7 B 9 10 1112 Jaaodogodo OOOUCOOOOUOoOo www Dj jT I JL I I www s LI JJ J J dC CLDLBOLL 222 o 000000 bai bai Sai Ooiman E EE 0000000000 E aw un Cs oy im JE 3E EIE TIE IE TE TE E IL E d wwe 4 IDIDIEIEILIEIEILIEICICUCU Figure 26 Tray Type V132 Figure 27 Tray Type DW96 4 2 2 2 Correction for Sample Position in X Y or Z Axes PAL Firmware Version 2 X X The following description of the correction is done by using the path to the Utilities section After selecting the Utilities function Trays verify whether the Tray Type assigned corresponds to the Tray placed into the Tray Holder If this is verified the vial positions can be checked at the three corners of the Tray This is activated by the F5 function key Menu Utilities Tray Select Tray Select Function Key F3 Move to 001 4 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Tray Tray 1 Scroll down 2009
3. 8 1 COM Port Settings The port settings are normally defined in Windows Control Panel Device Manager Ports The standard settings for the serial communication are shown in Figure 45 It is important to understand that the application e g PAL Loader Software does actively set the communication parameters and the baud rate is set for optimized use Communications Port COM1 Properties EA X Y Advanced Restore Defaults Figure 42 COM Port Settings A quick way to check whether serial communication with the PAL System can be established is to use the PAL Loader software For details see Addendum to PAL User Manual PAL Loader Software 62 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 8 2 Setting up LAN Communication Select the firmware Communication class by the following path Menu F3 Setup Communication Menu F3 Setup Communication See detailed description below under point 10 The system automatically searches for the MAC address when the Setup Communication window Is accessed MAC address is read from hardware Please wait 2009 04 17 16 07 07 tt E E lt re pa Figure 43 Reading the MAC Address from the Ethernet Communication Port This search for and reading of the MAC address takes approximately 10 to 15 seconds If successful the MAC A
4. Pressing the function key labeled Home will always return to the Job Queue menu 1 5 ESCape and STOP Keys Press the ESCape key to return to the previous menu Press the STOP key to abort the current Cycle Job or Job Queue 1 4 Scroll Knob and ENTER Button Rotate the outer knob to scroll through items in a menu list To select a highlighted item press the central knob ENTER button Then use the outer knob to scroll through available options for that item or to change a numeric value Then press the inner knob again to ENTER the displayed option The inner knob is also used for other operations that require an ENTER operation to continue or complete an operation PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 2 Methods 2 1 Creating Methods Methods can be defined by the user and assigned names up to eight characters in length Methods can be created copied edited and viewed from the Methods menu Methods can be viewed but not edited from the Job Queue menus Methods are created by either copying an existing Method or creating a new Method Menu Methods Method name Copy Method To copy a Method complete the sequence as follows You will be prompted to enter a name for the new Method Use the scroll knob and the left right arrow function keys F2 and F3 to select among alphanumeri
5. NC RIS bridged with Pin 4 Do not alter NC NC coO oo o o01 C6X4 h2 Connector SER2 PAL System Pin Signal Name GND RXD TXD NC NC 5V NC GND co o CO1 COX4 h2 Connector TERMINAL or SER3 PAL and xt System Pin Signal Name GND RXD TXD NC NC 5V NC GND co o O01 COG4 h2 108 PAL is a registered trademark of CTC Analytics AG Switzerland system COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt Installation and Operation Connector INTERFACE 1 PAL and xfSystem ce o ou A e rm x co 10 11 12 15 14 15 15 2229909900 22299900 1 Signal Name Pwr Outl Pwr Out2 SW Outl NO SW Outl1 COM SW Out2 NO Opto In1 TTL Inl GND Pwr Outl Pwr Out2 TTL In2 SW Out2 COM TTL In3 Opto In1 5V Connector INTERFACE 2 PAL and azSystem 15 O 99 9999Q 1 QOOQOQOQOQOQQOQOQOQOOQ ce o o A5 e m x 2 O NPN 14 19 20 2 22 25 24 25 PAL is a registered trademark of CTC Analytics AG Switzerland 25 Signal Name TTL In1 TTL In2 TTL In3 TIL Outl TTL Out2 TTL Out3 Opto In1 Opto In2 SW Out1 NO SW Out2 NO Pwr Outl Pwr Out2 5V GND Opto In1 Opto In2 SW Outl1 COM SW Out2 COM Pwr Out1 Pwr Out2 14 109 COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA S
6. SYSTEM User Manual Installation and Operation 2 Place the blue plunger holder into the injection unit 3 Place the syringe in the appropriate syringe adapter Pull the plunger out to approximately 20 of its length See Fig 37 I il EA Figure 37 Syringe and Syringe Adapter for Liquid Injections 4 f necessary loosen the plunger retaining screw in the plunger holder 5 Move the syringe installed in the syringe adapter partially into the Injection Unit First guide the needle into the upper needle guide and then into the lower needle guide Fig 38 6 Insert the plunger button into the plunger holder Allow the syringe adapter to click into place by magnetic force against the syringe carrier 7 Tighten the plunger retaining screw against the plunger button 8 Press Home The plunger moves down until it hits the mechanical stop This position is stored as the syringe s zero volume position The Injection Unit then returns to the HOME position Figure 38 Installing and Removing a Syringe for Liquid Injections 55 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 5 4 Removing a Syringe for L
7. Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt PA SYSTEM User Manual Installation and Operation Figure 51 Replacing MOTIO Board 8 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 2 CPU Board PNo APR CPU 1 Remove the right end cover of the X axis facing the rear of the chassis Remark This step is only necessary if the CPU board needs to be removed and the MOTIO Board remains in the system 2 Slide the CPU board out and towards the right end of the X axis This is necessary because the CPU board mates on its left side with a connector on the MOTIO board 3 Install the replacement board in the reverse order Figure 52 Replacing CPU Board PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 Control xt Board PNo APR Control xt The Control xtboard combines the MOTIO and the CPU boards in a single control board This board is required for the PAL COMBI xt System and operates only at PAL Firmware Version 4 1 X or higher 1 Follow the sequence shown in Figure 45 to detach and release the Control xfboard from its position inside the X axis As shown remove the dummy cover first 2 Carefully pull the two ribbon cable connectors upwards to fr
8. dzCol for Position 032 2009 05 06 16 07 20 Figure 31 Adjusting Column Inclination at Third Tray Corner Position The three corner points are now adjusted for a possible inclination of the Tray in any axis A possible deviation from an ideal axis position for the other vials caused by variance from the horizontal is interpolated by the system without further teaching For routine work using standard vials it is not necessary to compensate for a possible inclination as long as the vial detection is within the Z Tolerance range 4 2 3 Wash Station Wash1 Wash2 Reference Point If positions of other types of Wash Stations have to be taught follow the instructions in the HTS PAL User manual for the Fast Wash Station or for other types such as Active Washstation in the specific Addendum of the PAL User Manual Observe safety measures if a waste line has to be installed in combination with a gas chromatograph Do not position the waste line above a heated Zone yy p e A ash CD c 3 j Wash 1 SAS Wen l Waste Waste 2 Standard Figure 32 Wash Station Wash1 Wash2 Reference Point For the Wash Station the reference positions are the two holes in the wash vial caps see Fig 32 Wash1 and Wash2 The lower needle guide should be centered in these holes with the bottom of the lower needle guide lightly touching the surface of the caps 50 PAL is a registered trademark of CTC Analyti
9. 3uimumj gul sn 4 JAS pa lui Id 9G u ad paads pelu 0 peluj 80 annd paads H dwa aduuAS aui HO By aui uo Isy paads 18y awy jeqnouj dwa yeqnou DUNO ajdwes 3DNIMAS 71 9 anedspeay 3p4 pota joypejnueui 0j JaJnejnueul Jopelur wo sapp anjeA 10paluj sayn sopaluy uonejauag a paaw opalui JOY ui pajeau apaau awn Jo yua sejduies snoosi Ajuiy 10j pasn si Aep uoipaluj ad sanuejod 1uaJajip A WANXS JO sjusuoduloo YIA sajdues 104 pasn SI Z JU3A 0S ULAN 34d a8uuKs saan s M 0S os a8uus Tri OL 104 paads pal3 0 0 0 507 015 Scot su sw 0 sw 0 sw 0 Moas Sig sim os LUD Lu uy 39 sc sc sc E E E mol SO Wor mol mol mol mol iot lur 55 lur 55 lur 55 SuisuodeA usn j PIO palur Ald SUWUN O paypeg uomeluj uuinjo uo uoralu uds SIWELA POAN Tyd papuauuo ay INS U 15d LAS U 18d pa luris ed ful aug paads palu o patu pa dnjna SOWONS paads l4 UNION jd ds up ad TNS UD 3Jd INS UPD 3Jd SLUNIOA Jy auno ajduues 3ONIMAS TDA pinor apo Polya PAL is a registered trademark of CTC Analytics AG Switzerland Figure 61 PAL COMBI x Firmware Overview Page 4 COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 5 External Connectors for PAL and xt System Connector SERT PAL and xfSystem Pin Signal Name NC RXD TXD DTR bridged with Pin7 Special grounding do not alter GND
10. Check flush gas for impurities Contact your CTC Analytics representative 9l PAL is a registered trademark of CTC Analytics AG Switzerland note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation H Replacing Parts Safety Warning Always disconnect the power cord s from the power supply or from the various power supplies if optional devices are installed before attempting any type of maintenance Capacitors inside the instrument may still remain charged even if the instrument Is turned off To avoid damaging electrical parts do not disconnect an electrical assembly while power is applied to the PAL system Once the power is turned Off wait approximately 30 seconds before you disconnect an assembly 1 MOTIO Board PNo APR Motio 1 Follow the sequence shown in Figure 51 to detach and release the MOTIO board from its position inside the X axis As shown remove the dummy cover first Slide the MOTIO board to the left side This is necessary because the board mates on its right side with the connector on the CPU board 2 Carefully pull the two ribbon cable connectors upwards to free them from the board 3 Install the replacement board in the reverse order After reconnecting the two ribbon cables to the board ensure that the cables are folded flat and can slide inside the X axis without damage 02 PAL is a registered trademark of CTC Analytics AG
11. Valve Drives The name of an Object List starts with the Module part number with variants added e g first or second Stack The name of the root folder includes the revision which is dependent on the firmware version e g Object Lists Rev K for firmware 2 X and 3 X Object Class Each Object belongs to an Object Class e g Syringes Trays Injectors The Object Class defines the Items of an Object Object Item An Object contains several Items which can be numerical values with a physical unit e g X Y Z Position Penetration Syringe Scale Length Syringe Volume or references to other objects Note that the term Parameter is reserved for ATOM Parameter PAL Firmware commands to be used for a PAL Cyde or Macro 89 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Objects Objects are data structures describing the properties of physical modules Certain modules e g a Stack require several objects Module PAL hardware module either part of a standard PAL configuration e g COMBI PAL HTS PAL or an optional addition e g Cooled Stack MALDI Tool Dilutor The term Module is intentionally used to differentiate from Object which is reserved for the PAL Firmware Object 90 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt P
12. WEEE regulations e Directive 2002 95EC RoHS e Directive 2002 96 EC WEEE CTC Analytics AG reserves the right to make improvements and or changes to the product specifications without notice 52 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 2 Product Warranty 2 1 Statement of Limited Product Warranty What does this Warranty cover CTC Analytics AG warrants only that its products comply with CTC Analytics AG specifications This warranty covers defects in or failures of the Autosampler and major accessories such as Agitator Stack Coolers or Valve Drives occurring as a result of normal use or manufacturing defect What is NOT covered by this warranty This warranty does not cover defects or failures resulting from damage caused by accident misuse or abuse such as Improper or unauthorized service or repair Failure to follow the operating instructions provided by CTC Analytics AG Improper or insufficient ventilation Force Majeure No liability for events beyond its reasonable control including but not limited to fire storm flood earthquake explosions riots strikes labor disputes transportation embargoes or other contingencies beyond the control of CTC Analytics AG i e acts of God or Force Majeure Exposure to corrosive compounds The warranty does not cover part
13. changes possible If Gateway Is used in network the Gateway IP address switch hub has to be entered The unique MAC Address for the PAL Xt System is read and entered into this PAL xt communication protocol This is a read only parameter field PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation Remarks If serial communication Is selected all other parameters in PAL xf Firmware Communication dass are Ignored It is advisable to use a fixed IP address set the DHCP parameter to Off In this mode the PAL xt System always has the same fixed IP address Troubleshooting is easier for the network administrator See also special remarks below The IP address is a fixed PAL xt System address The IP address is often provided by the network administrator The SubNet mask must be provided by the network administrator The network administrator must be informed about this setting The port must be cleared of firewall protection to enable communication Gateway is used if independent networks need to communicate interactively Communication within the network is provided by a hub or switch defined as Gateway The MAC address is provided by the Ethernet communication hardware The address is also visible beside the read out from the terminal on the label affixed to the Ethernet
14. in the X axis of a column see Fig 25 for details dyCol Correction of any inclination of a Tray plate in the Y axis of a column see Fig 25 for details dzCol Correction of any inclination of a Tray plate in the Z axis of a column see Fig 25 for details Standby Temp Sets the temperature for the Agitator during standby mode e g before or after a Job Job Queue is processed Indicates the actual agitator temperature Speed Sets the Agitator spinning speed Usually it has to be set as high as possible but not so high that the liquid does not move up to the septum Check the correct speed by inserting a spacer below the vial inside the agitator so the liquid level is visible Try different speeds and monitor the liquid level Agi On Time Sets how long the Agitator spins in one direction Agi Off Time Sets how long the Agitator stops before it reverses the direction of spin If this value is set to 0 only the clockwise direction with no interrupts will be used Available for Agitator only Items displayed in Italic are available beginning with PAL Firmware Version 4 1 X A staggered pattern is selectable in firmware class Tray Type For details see section F Description and Installation point 9 3 1 Tray Type 2 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4
15. see Appendices point 3 Injection Cycles and point 4 Flow Chart Firmware Overview HS Inj Standard cyle for GC headspace technique Details on method parameters see Appendices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview SPME Standard cyde for GC SPME technique Solid Phase Micro Extraction Fiber technique Details on method parameters see Appendices point 4 Flow Chart Firmware Overview TTS The two cycles are dedicated cycles for dissolution applications TTS cycle is used Sotax for transdermal patches and the Sotax cycle is used for tablet dissolution Items displayed in italic are available beginning with PAL Firmware Version 4 1 X Standard injection cycles are selectable and activated directly from the PAL System Any deviation from a standard cycle a Cycle Composer Macro or Cycle Editor ICC CE Cycle must be programmed for customized requirements In PAL Firmware versions lt 4 0 the standard cycles are grouped in Item Cycles 70 PAL is a registered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 9 2 2 Service The following Service items may be changed by selecting the particular Item Item Check Motors Test Head i Test Switches Description Every stepper motor can be moved separately This function is useful to check out a specific moto
16. 06 16 16 07 20 Figure 28 Utilities Function Trays The Injection Unit moves to Tray position no 001 With the help of the inserted syringe is it possible to check the optimized position of the needle tip matching with the vial or well position In case the position is off make corrections in X Y or Z positions Continue with function key F5 to move to the next corner to verify this position If the position matches continue with function key F5 to move the unit to the third corner position If a deviation of the ideal position is observed in second or third corner the corrections have to be made on the position no 001 The offset is bound to position no 001 Activating one of the parameters Offset X Y or Z axis at the positions of second or third corner can bend the syringe needle 4 2 2 5 Correction for Inclination in X Y or Z Axes PAL Firmware Version 4 1 X or higher The following description of the correction is done by using the path to the Utilities section After selecting the Utilities function Trays verify whether the Tray Type assigned corresponds to the Tray placed into the Tray Holder If this is verified the vial positions can be checked at the three corners of the Tray This is activated by the F5 function key Menu Utilities Tray Select Tray Select Function Key F3 Move to 001 40 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL
17. 47 Figure 48 Figure 49 Figure 50 Figure 51 Figure 52 Figure 53 Figure 54 Figure 55 Figure 56 Figure 57 Figure 58 Figure 59 Figure 60 Figure 61 Selecting Setup in Extended User Mode Selecting Objects in Extended User Mode Pattern Tray Type Regular Pattern Tray Type Staggered Pattern Tray Type Staggered Replacing MOTIO Board Replacing CPU Board Replacing Control xt Board COMBI PAL Firmware Overview Page 1 COMBI PAL Firmware Overview Page 2 COMBI PAL Firmware Overview Page 3 COMBI PAL Firmware Overview Page 4 PAL COMBI xt Firmware Overview Page 1 PAL COMBI xt Firmware Overview Page 2 PAL COMBI xt Firmware Overview Page 3 PAL COMBI xt Firmware Overview Page 4 PAL is a registered trademark of CTC Analytics AG Switzerland note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation D How to Use this Manual This User Manual covers the COMBI PAL and PAL COMBI xt Systems Issues specific to the PAL COMBI xt model have been emphasized as such Follow the description given in this manual to Install and operate the PAL model LHX PAL which differs only by its X axis length of 120 cm as compared to 80 cm for the COMBI PAL model and thus is not handled separately Thus model PAL LHX xtis also not handled separately Unless stated otherwise this manual refers to all COMBI PAL models The manual is divided into following major s
18. COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation F COMBI PAL Description and Installation 1 General System Overview Liquid Syringe and syringe adapter Flush Gas Pressure Regulator Injection unit Z Axis ZzEkES I Plunger Holder Headspace Syringe and Heater meseeeee i ceocccecoc i DODO DO t Wash Station Tray Holder Tray Figure 8 COMBI PAL Major System Components The standard COMBI PAL includes the following hardware 1 X Y axes assembly Injection Unit Z axis 1 Flush Gas Pressure Regulator 1 Tray Holder Tray for 1 mL 2 mL vials 1 Tray Holder Tray for 10 mL 20 mL vials 1 Syringe kit for liquid injections 1 Syringe kit for heated Syringes 1 Heated six position Agitator 1 Wash Station for two different solvents 1 Keypad terminal 1 Safety Guard 1 GC mounting kit optional PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation 1 1 Specifications 1 1 1 Sample Capacity e 98x2mLl vials per Tray e 200x1 mL vials per Tray e 32x10mL 20 mL vials per Tray up to three Trays can be configured 108 x 2 mL vials per Stack Cooler 2 DW 2 x 54 vials 216x2 mL vials per Stack 4 DW without cooling heating 2 Micro Deepwell plates per Stack Cooler 2 DW 4 Micro Deepwell plates per Stack 4 DW 1 1
19. Define Object s correctly See section F Describing and Installing point 4 1 Defining Object Positions Redefine Z value for the affected Object See section F Describing and Installing point 4 1 Defining Object Positions Switch OFF PAL and check connection between PAL and Agitator Agitator Connection cable Change connection cable MOTIO Board defective Replace MOTIO or Control x Board o0 PAL is a registered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation Headspace specific Symptoms Sample peaks or responses are not reproducible Peak distortion or tailing Excessive carryover between samples Unexplained chromatographic peaks Faulty temperature read out e g value 99 C Possible Cause Dirty syringe Recommended Action Increase method value Syr Flushing Set method value Syringe Temp 10 C above Incubat Temp Syringe pressure differences Increase Pullup delay value Vacuum created in sample vial Reduce sample volume Improperly crimped vials Check vial cap by attempting to rotate manually Loose caps may cause selective loss of more volatile components from sample Adjust crimping tool correctly None or too low Syringe flush gas pressure Method Parameters Method Parameters Needle penetration speed in GC in
20. Galaxie or Chromeleon accomplish synchronization of the Ready Start signal done directly via software control 58 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation RS232 LAN A synchronization cable is not required and the Sync Signal Start must be set to Immediat Menu Setup Objects Out Signals Injected Destination SW Outl To assign a physical Event e g SW Out 1 to an existing signal Object Injected complete the following menu selections PAL Systems are shipped with all Cycle events pre defined as shown in the table below If a different physical signal needs to be used then it will be necessary to assign a new Event to the Object signal If a signal is to be ignored then its corresponding Event must be set to Immediat Standard Assignment for Description Default INTERFACE COMBI PAL Injection Cycles Events 1 Pin Start JobQueue Sync Signal Start a Job Queue Job Immediat Prerequisite is a defined Job with a method assigned Useful for automated unattended timed sampling Start Sync Signal Start a cyde Input from GC or data I 7 system Pett 8 GND Start2 Sync Signal Continue the cycle GC Dual for Immediat the second sample Ignore Inject Sync Signal Inject Immediat READY to INJECT Ignore Inject2 Sync Signal Inject the second sampl
21. PA SYSTEM User Manual Installation and Operation 3 2 2 Installing the Keypad Terminal Figure 14 Installing the Keypad Terminal Install the safety shield on the left and right sides to the outside of the X axis Use the provided longer thumbscrew on the side where you plan to install the keypad Install the Keypad mounting bracket on either the right or left side of the X axis Connect one end of the white coiled cable Part No SS8J 700 to the Keypad and the other end to the TERMINAL SER3 interface jack on the rear side of the X axis For details see Figures 19 or 20 Do not interchange the Terminal with LAN cable connectors 4 Place the Keypad terminal onto its mounting bracket 3 2 3 Installing the Power Supply l Locate the power supply the DC power cable Part No RS3M 2000 and the AC power cable Set the power supply switch to the OFF position Connect one end of the DC power cable to the power supply and the other end to the POWER connector at the rear side of the X axis Connect the female end of the AC power cable to the power supply Then connect the male end to an AC power outlet Before proceeding with the remaining steps make sure the power to the PAL System is switched off 58 PAL is a registered trademark of CTC Analytics AG Switzerland note note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Insta
22. PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Menu Setup 2009 05 06 16 07 20 Figure 7 Selecting Setup Functions 7 1 Sounds Item Message Box Warn Move End Cycle End Job 7 2 Time Item Year Month Day of Month Hours Minutes Seconds Description A specific dual beep tone signals that a pop up window Message Box for user intervention appears on the screen This beep signal can be turned on or off A beep sound is heard at the start of the PAL movement It is advisable to keep this function turned on for safety reasons A beep sound is heard at the end of a cycle Select as desired A beep sound is heard at the end of a cyde Select as desired Description The year can be set for the PAL internal clock The month can be set for the PAL internal dock The day can be set for the PAL internal clock The hours can be set for the PAL internal clock The minutes can be set for the PAL internal dock The seconds can be set for the PAL internal dock After setting or resetting the date and time use the function key FT Set Time to store 7 5 Objects The various PAL Firmware Object classes can be selected and the functions most used by the user are directly accessible For detailed listing see PAL Firmware overview in the appendices 2 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL
23. PAL system The PAL Loader software has always been a part of the PAL System The following PAL Loader Software versions are in use PAL Loader Version 1 0 1 PAL Loader Version 1 1 1 A new PAL Loader software Loader version 2 1 1 or higher has been developed for the PAL xfsystem The PAL xtsystem requires PAL Firmware 4 1 X or higher which calls for the new Loader software The new PAL Loader Software is backwards compatible The software can be used for all PAL Firmware versions The name of the exe file has been changed intentionally to avoid conflicts when old and new versions are installed on the same computer e PAL Loader version 1 1 1 PALLOAD exe PAL Firmware version up to and including 3 X X e PAL Loader version 2 1 X PALoader exe PAL Firmware version as of 4 1 X but backwards compatible with lower versions Details are described in Addendum to PAL User Manual PAL Loader Software Version 2 1 X Installation and Operation 60 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 7 2 PAL Object Manager Software The PAL Object Manager software is used to load a new PAL Firmware Object to a system after a new module hardware module has been added to the PAL System The PAL Object Manager software is the software used to handle the so called PAL Object List The software versi
24. Retract needle from injector MotorZ Motors Inj Penet Spd 17 Clean syringe with solvent from Pst Cln Slv1 SYRINGE Syringes Fill Speed Wash1 selected number of times Eject Speed Wash Wash Stations Needle Penetr Clean Volume Waste Injectors Needle Penetr 18 Clean syringe with solvent from Pst Cln Slv2 SYRINGE Syringes Fill Speed Wash2 selected number of times Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Waste2 Injectors Needle Penetr 19 Move to Home position Home Positions PAL is a registered trademark of CTC Analytics AG Switzerland 92 syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 1 1 GC Inj Cycle Method Parameter Description Parameter CYCLE SYRINGE Sample Volume Air Volume Pre Cln Slv1 Pre Cln Slv2 Pre Cln Spl Fill Volume Fill Speed Fill Strokes Pullup Del Inject to Inject Speed Pre Inj Del Pst Inj Del Pst Cln Slv1 Pst Cln Slv2 Description GC Inj Selected syringe Selected sample volume Volume aspirated after the syringe needle is moved out of the sample liquid Pre injection syringe wash strokes with Solvent 1 Pre injection syringe wash strokes with Solvent 2 Pre injection syringe wash strokes with Sample Fill Volume used for all filling strokes except the last one Speed of plunger movement used in all syringe filling operations Number of fill strokes Delay time between sample pullup and ejection Selected Injec
25. System Power Supply Input line voltage Grounded AC 100 to 240 V Input line frequency 50 60 Hz Input power 4A Output voltage 36 VDC Output current 4 16A a Protection Class I Protection class describes the insulating scheme used in the instrument to protect the user from electrical shock Class identifies a single level of insulation between live parts wires and exposed conductive parts metal panels in which the exposed conductive parts are connected to a grounding system In turn this grounding system Is connected to the third pin ground pin on the electrical power plug b Over Voltage Category II Over Voltage category Il pertains to instruments that receive their electrical power from a local level such as an electrical wall outlet c Pollution Degree 2 This is a measure of pollution on electrical circuits that may produce a reduction of the dielectric strength or surface resistivity Degree 2 refers to normally only non conductive pollution Occasionally however a temporary conductivity caused by condensation must be expected d Moisture Protection Normal IPXO IPXO means that there is NO Ingress Protection against any type of dripping or sprayed water The X is a place holder to identify protection against dust if applicable 50 PAL is a registered trademark of CTC Analytics AG Switzerland syste 1 4 Physical Specifications Parameter COMBI PAL Systems Height Depth Width Weight CO
26. Unit to the Y axis 2 Connect the ribbon cable A protruding from the front end of the Y axis to the corresponding connector on the Injection Unit see Fig 11 Figure 11 Connecting the Injection Unit Ribbon Cable 36 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 Hold the Injection Unit in place against the Y axis Make sure the two locating pins on the Y axis fit into the two guide pin holes on the Injection Unit 4 Place one of the screws onto the end of the supplied Torx driver Slide the clear plastic cover on the Injection Unit all the way to the top Locate the three large holes in the black anodized frame attached to the Z axis inside the Injection Unit Slide the frame upwards until the top hole is centered on the top threaded hole at the end of the Y axis Insert and securely tighten the Torx screw A See Fig 12 Figure 12 Inserting the Injection Unit Mounting Torx Screws 5 Install the two remaining Torx screws B C in the left and right mounting holes respectively It may be necessary to move the elastic cord slightly to the left to insert the Torx screw C into the right hand hole Figure 13 COMBI PAL with Injection Unit and Standalone Supports 57 PAL is a registered trademark of CTC Analytics AG Switzerland note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt
27. User Manual Installation and Operation 3 2 5 Installing the Wash Station Attach the Wash Station assembly to the X axis Figure 16 Installing the Wash Station If other types of Wash Stations are installed follow the instructions in the HTS PAL User Manual for the Fast Wash Station or for other types such as Active Washstation in the specific Addendum of the PAL User Manual Observe safety measures if a waste line has to be installed in combination with a gas chromatograph Do not position the waste line above a heated zone 3 2 6 Installing the Agitator 1 Loosen the two Torx screws on the two Agitator mounting brackets Figure 17 Installing the Agitator 40 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 2 Attach the Agitator to the left or right end of the X axis Install it with the mounting clamp teeth fit into the grooves on the bottom of the X axis Do not position the Agitator in the middle of the X axis The Agitator must be installed on the right or left side Crossing over the Agitator with a 20 ml vial would cause a collision 3 Be sure that the clamps fit completely into the grooves Alternately tighten the two Torx screws until the two mounting clamps are firmly in place 4 Double check whether the Agitator clamps are correctly attached to the X axis See Fig
28. a replacement of the injector system The actual number of injections is displayed If the counter reaches the set limit PlgStrokeLim a warning is displayed at the next start of a job run Inject Limit The same counter is used for valve switches HPLC technique Inject Limit An upper limit for the number of injections can be set note Counters for the plunger movement and injector penetrations are available with PAL Firmware version 2 5 X or higher 6 4 Free Objects Free Items In addition to the core software the PAL Firmware contains data for the Firmware Objects There are different classes of Objects such as Syringes Trays Tray Holders etc Each class of Objects contains Items The items contain the actual data such as X Y Z positions The data are stored in a flash memory backed up by a battery To optimize RAM and Flash memory use a certain section of memory has been reserved by the software for each of the Objects and Object Items The percentage shown in the Info section provides an indication as to how much of the reserved software space Is still available 7 Setup Functions The Setup functions selectable from the Menu screen allow accessing various functions for the PAL System The Sound Time and Objects are basic functions used at installation or if changes have been made over time 26 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL
29. important that the power supply ies be in a location where the power ON and OFF switch is accessible and easy to operate and where it is possible to unplug the AC power cord from the power supply wall outlet in case of emergency PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Other Hazards 3 Fi x 3 To avoid injury during PAL System operation keep your hands away from the syringe A Do not operate the PAL System without the safety shield The safety shield must be installed for safe operation A To avoid injury observe safe laboratory practice when handling solvents changing tubing or operating the PAL System Know the physical and chemical properties of the solvents you use See the Material Safety Data Sheets from the manufacturer of the solvents being used A Use caution when working with any polymer tubing under pressure e Always wear eye protection when near pressurized polymer tubing Do not use polymer tubing that has been severely stressed or kinked Do not use polymer tubing in particular no PEEK or Tefzel tubing when using tetrahydrofuran THF dimethylsulfoxid DMSO chlorinated organic solvents concentrated mineral acids such as nitric phosphoric or sulfuric acids or any compounds related to the above A Do not use vials without a sealing cap microtiter o
30. of the sample liquid Injector used for first injection Time difference between first and second injection Offset from first sample number to second sample number Sample volume for first injection Volume aspirated after the syringe needle is moved out of the sample liquid Injector used for second injection Wash cycles with Wash1 before first sample is aspirated Wash cycles with Wash2 before first sample is aspirated Rinse cycles with sample before first sample is aspirated Wash cycles with Wash1 before second sample is aspirated Wash cycles with Wash2 before second sample is aspirated Wash cycles with Wash1 after second sample is aspirated Wash cycles with Wash2 after second sample is aspirated Fill Volume used for all Filling strokes except the last one Speed of plunger movement used to aspirate sample Number of filling strokes Delay time between sample pullup and ejection Speed of plunger movement used during sample injection Delay time prior to sample injection Delay time after sample injection 99 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt User Manual Installation and Operation 6007 aun TSZ emua 5 uorsiva WA SAS Ty q JENPIAIDU _YDd2a JO uonein8ijuo aempey ay uo spuadap 1no e ayy 4 1 COMBI PAL Flow Chart based on PAL Firmware Version 2 5 x 4 PAL Firmware Overview system Sog Aqpuels sog auu Sid MalAJ9AO IY u
31. or other techniques Other techniques or areas of application are also possible XYZ robotic systems are typically used where vial sample transport liquid handling or sample preparation need to be automated Known fields of application include tablet dissolution and viscosimetry The users will to a large extent themselves determine which substances compounds or liquids the system will come into contact with 6 3 Disclaimer and Restrictions on Use The PAL System boundary represents a significant limitation of the manufacturer s responsibility Submitting and placing samples vials well plates or other sample holding devices inserting syringes selecting reagents and wash solvents are together with any other preparative tasks solely the responsibility of the user Any possible sample mix up or omission of an item cannot be detected or prevented by the PAL System The PAL or PAL xt System boundary is defined when the sample is placed in the system and by the final prepared and primed system The use of the PAL and PAL xt Systems for any invasive technique on human beings or animals is explicitly excluded In addition any application in an environment where explosion protection is required Is specifically prohibited for the PAL and PAL xt Systems 112 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 7 PAL Accessori
32. specific legal rights and you may also have other rights which vary from country to country or from state to state How does this Warranty relate to Warranty Statements of various Distributing or OEM Partners This warranty statement from CTC Analytics AG gives the user specific legal rights If the warranty statement from your purchaser distributor or OEM is broader the added value of that warranty will be valid for the user This however this is not the responsibility of CTC Analytics AG but solely that of the particular distributor or OEM partner 54 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 Installation 3 1 Unpacking the Components A COMBI PAL system is shipped in one box The box contains the X Y axes assembly the Injection Unit the heated Agitator the Keypad Terminal GC mounting kit optional connecting cables power supply heated Syringe kits Tray Holders Trays Wash Station assembly Safety Guard and miscellaneous parts 1 Open the box and first remove the accessory boxes and the Injection Unit before attempting to remove the X Y axes assembly 2 Carefully lift the X Y axes assembly and remove it from the box Hold the Y axis in place while the assembly is being removed from the box Set the X Y axes assembly on a bench 3 Unpack the remaining small boxes and any ot
33. the plunger up to the position specified by this item For normal use accept the default values Init Syr at The syringe plunger can be initialized referenced at Home or Waste position If samples with a high danger of toxicity are treated it is advisable to use the Waste position for referencing Inj Signal An Autosampler is usually the master in a total chromatographic system The other components send a Ready signal to enable a start of the Autosampler At the moment of injection a Start signal is sent from the PAL System to the other component s of the entire system With this Inj Signal item the status at which the signal is sent out can be defined The syringe is filled with the defined sample volume PlgUp Start signal at the moment the plunger starts moving down for injection PlgDown Start signal at the moment the plunger reached zero point from syringe recommended for large volume injection but not in combination with Agilent Chemstation ValveSw Start signal sent at the moment the injection valve is switched HPLC technique Prelnj Start signal sent at a time before injection as with a sample preparation device started before a GC or LC system Minus time relative to chromatographic start time al 69 PAL is a registered trademark of CTC Analytics AG Switzerland note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Vial PrePre
34. 00000 o ICIEIEIEIEIHICIEIEIE deiectis e goood F OOOOOOOoooo0o Joha GHUOO0000000 ww ICIEICILICICIL IL IE IC IEYE Figure 48 Pattern Tray Type Regular Row Pattern Type Stagerd The sample or well positions are arranged in a staggered pattern Offset of second Row Is shifted by 50 of hole pattern Column K m a ff Row 1234567 89 101112 t oc m A mam o mc a nm 8 D m e E f m F o i A G Figure 49 Pattern Tray Type Staggered Row Pattern Type Staggrd The sample or well positions are arranged in a staggered pattern Offset of second Row Is shifted by 50 of hole pattern Column 2 eC o U Ww Row VI ww f o w 123 45 67 8 9 101112 n O D A Ow g B d j 2 w w g ella ow D ww Ww E Do 8 DA F ww y eC wo G SoH L Figure 50 Pattern Tray Type Staggered PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation syste 9 3 2 Trays The following object Tray Ite
35. 2 Thermostatted Sample Tray e Tray Cooler optional 4 C 70 C e Stack Cooler 2 DW optional 4 C 40 C 1 1 5 Liquid Injection Range 1 uL 10 uL with standard 10 uL syringe Down to 0 1 uL with sandwich technique or Optional 1 2 uL syringe Up to 50 500 uL with optional 500 uL syringe 1 1 4 Headspace Injection Range e 250 2500 uL with standard 2 5 mL heated syringe e Down to 100 uL with optional 1 0 mL heated syringe e Up to 5000 uL with optional 5 0 mL heated syringe 1 1 5 Heated Syringe Temperature e 55 C 150 C selectable in 1 C increments 1 1 6 Incubator Agitator e Six heated vial positions for 2 10 20 mL vials e Temperature range 35 C 200 C selectable in 1 C increments e Orbital interval shaking speed 250 750 rpm 29 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation 1 1 7 Replicate Injections e 1 99 from one vial 1 1 8 Minimum Sample Volume e 1uLfroma3uL sample in 1 mL tapered micro vial in liquid injection mode 1 2 Performance Specification The performance specifications are available as part of a specific separate document 1 5 Electrical Specifications Parameter Requirement Protection dass Class Over voltage category Category ll Pollution degree p Moisture protection Normal IPXO Voltage 56 VDC Current 3 2A Fuse 16 5 A 250 V PAL
36. 3 Injector After selecting the particular Injector to be accessed the following Functions are available Function Key Description F3 Movto Inj The Injection Unit moves to the selected injector position With this function e g the injectors GC Inj1 Waste Waste2 and Flush can be accessed By selecting the parameter Needle Penetr on the same screen the Injector Needle Penetration value can be checked or changed F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Injector item may be changed by selecting the particular item Item Description Needle Penetr By selecting the parameter Needle Penetr the Injector Needle Penetration value can be checked and or changed To ensure reproducible sample injections and minimize carryover it is critical that the needle penetration depth be accurately set 4 4 Wash Station After selecting the specific Wash Station the following functions are available Function Key Description F3 Movto Wash The injection unit moves to the selected Wash Station port By selecting the Parameter Needle Penetr on the same screen the Wash Station Needle Penetration value can be checked or changed F4 HOME The injection unit moves to its HOME position and the Job Queue Menu is displayed Item Description Needle Penetr By selecting the Parameter Needle Penetr the Wash Station Needle Penetration value can be checked and or changed Rinse Time If rinse
37. 6 5 Syringes 54 5 1 Selecting Syringes 54 5 2 Syringe Priming for Liquid Syringe Types only 54 5 3 Installing a Syringe for Liquid Injections 54 5 4 Removing a Syringe for Liquid Injections 56 5 5 Installing a Syringe for Headspace Injections 56 5 6 Removing a Syringe for Headspace Injections 57 6 Interfacing the COMBI PAL to Other Devices 58 6 1 Synchronization and Output Signals 58 7 PAL System Software 60 7 1 PAL Loader Software 60 7 2 PAL Object Manager Software 61 8 Establishing Communication with Computer 62 8 1 COM Port Settings 62 8 2 Setting up LAN Communication 63 8 5 Troubleshooting for Serial or LAN Communication 66 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Table of Contents contd 9 Special Functions 67 9 1 How to Access Extended User Mode 67 9 2 Section F3 Setup 68 9 3 Section F3 Setup Objects 72 G Troubleshooting PAL and PAL xt System 80 H Replacing Parts 82 1 MOTIO Board 82 2 CPU Board 84 5 Control xt Board 85 4 Agitator 86 5 Injection Unit 86 6 Wash Station 86 I Maintaining the PAL System 87 J Appendices 88 1 Definition of Terms 88 2 Naming Convention 9 3 COMBI PAL and xfInjection Cycles 92 3 1 COMBI PAL GC Inj Cycle 92 3 2 GC PAL GC InjS Cycle 94 3 3 COMBI PAL HS Inj Cycle 96 3 4 COMBI PAL GC Dual Cycle 98 4 PA
38. 9 5 Connect the supplied Agitator control cable trom AUXI to the connector on the rear side of the Agitator 3 2 7 Installing a Tray Holder 1 Loosen the two Torx screws on the two mounting clamps located on top of the Tray Holder legs g Figure 18 Installing a Tray Holder 2 Install the Tray Holder with the mounting clamp teeth fit into the grooves on the bottom of the X axis 3 Be sure the clamps fit completely into the grooves Alternately tighten the two torx screws until the two mounting clamps are firmly in place 4 Double check if the two Tray Holder clamps are correctly attached to the X axis 4 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 3 Electrical Connections note Always switch OFF the PAL power supply before connecting or disconnecting any COMBI PAL accessories cable Do not interchange the Terminal with LAN cable connectors Before defining the COMBI PAL object positions make sure the Agitator and Keypad Terminal are correctly connected to the COMBI PAL X axis rear side See Figures 19 or 20 Figure 19 Electrical Connections for COMBI PAL System ANIM sy 2 2 E Oo oc Figure 20 Electrical Connections for PAL COMBI xt System PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI
39. A SYSTEM User Manual Installation and Operation 2 Naming Convention This section recommends the standard naming convention for COMBI PAL Trays Tray Types and Tray holders Following these conventions will allow the COMBI PAL to be pre configured for certain applications will simplify software backups and application development and will improve technical support and training Tray Type Tray Description VT200 Vial Tray 200 positions 10 x 20 For 7 mm micro vials 1 mL VT98 Vial Trays 98 positions 7 x 14 For 12 mm vials 2 mL VT78 Vial Tray 78 positions 6 x 13 For 7 mm micro vials 1 mL opposite side of 98 positions Tray VI54 Vial Tray 54 positions 6 x 9 For 12 mm vials 2 mL VT21 Vial Tray 21 Positions 7 x 14 For 12 mm vials 2 mL opposite side of 32 positions Tray VI22 10 Vial Tray 32 positions 4 x 8 For 25 mm headspace vials 10 mL VT32 20 Vial Tray 32 positions 4 x 8 For 25 mm headspace vials 20 mL MT96 Standard 96 position shallow microplate DW96 Deep well 96 position microplate MT384 High density 384 position shallow microplate 9 PAL is a registered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 COMBI PAL and xt Injection Cycles 5 1 COMBI PAL GC Inj Cycle No Processing Step Method Object Class Used Object Pa
40. BI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation A Safety Information General Considerations A The PAL System User Manual and the corresponding Addendum for a specific module must be consulted by the user under all circumstances before a unit is put in use Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment The user shall be made aware that if the equipment Is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired When using the PAL System follow the generally accepted procedures for quality control and methods development When using the PAL System in the field of chromatographic analysis if a change in the retention of a particular compound in the resolution between two compounds or in peak shape is observed immediately determine the reason for the changes Do not rely on the separation results until you determine the cause of a change Electrical Hazards A Every analytical instrument has specific hazards so be sure to read and comply with the following precautions They will help ensure the safe long term use of your PAL System The installation category over voltage category for this instrument is Level II The Level Il category pertains to equipment that receives its electrical power from the lo
41. CInSlv1 Slv2 and PreCInSpl Syringe pressure differences Increase Pullup Delay value Vacuum created in sample vial Reduce sample volume in sample vial Use setting under F3 Setup System PrePressureVial Highly volatile solvent Use gastight syringe and sample tray cooling Not properly crimped vials Method Parameters Dirty syringe Check vial cap by attempting to rotate by hand Loose caps may cause selective loss of lighter components from sample Adjust crimping tool correctly Check recommended Method Parameters in Flow Chart Firmware Overview Appendix 4 The following parameters are crucial and should be verified first Hill speed Pullup delay Injection Speed Post Inj Delay Increase Pst Inj Slv1 Slv2 values in method Use PreCInSlv1 Slv2 and PreClnSpl Check and if necessary replace vial septa from Wash Waste vials Inappropriate wash solvent s Use appropriate wash solvent s Wash Solvent and Waste vial septa too tight Object s not defined correctly Incorrect Z value for the affected Object e g GC Inj1 THldr1 or Agitator Agitator not connected Check if the syringe fills completely when washing If several deaning cydes are programmed and many samples are processed then vacuum in the Wash Solvent vials and overpressure in the Waste vials may build up Cut away a segment of the Solvent and Waste septa to assure equalization of pressure
42. COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation PAL User Manual Installation and Operation COMBI PAL PAL LHX PAL COMBI x PAL LHX xf Printing History Editon 1 January 1999 Software Version 2 0 Edition 2 April 2003 Software Version 2 0 Edition 5 August2005 Software Version 2 0 Edition 4 October 2009 Software Version 2 5 to 4 1 X Editon 5 March 2010 Software Version 2 5 to 4 1 X Edition 6 June 2010 Software Version 2 5 to 4 1 X Original Instructions CTC Analytics AG reserves the right to make improvements and or changes to the product described at any time without notice CTC Analytics AG makes no warranty of any kind with regard to this product including but not limited to implied warranties of merchantability and suitability for a particular purpose In no case shall CTC Analytics AG be liable for any coincidental or consequential damage in connection with or arising from the use of this document 2009 CTC Analytics AG All rights reserved Neither this publication nor any part hereof may be copied photocopied reproduced translated or reduced to electronic medium or machine readable form without the prior written permission of CTC Analytics AG except as allowed under copyright laws CTC Analytics AG acknowledges all trade names and trademarks used as the property of their respective owners PAL is a registered trademark of CTC Analytics AG Switzerland COM
43. FONIMAS JONIMAS JONIMAS 1 mods 7 A C umh 1425 vex TOO TDO TDO TOO ond EA Cosm opal rer ias 9 peH s z ca Klee 9 sum sog 2 nua Figure 54 M IAJ AQ 9JeMUUMH IgIAOO nasas Ve PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt PA SYSTEM User Manual Installation and Operation M E a uu The standard software does not include every object as shown in the overview The layout depends on the hardware configuration for each individual PAL System Revrsaon G Firmware 7 5 7 June 2008 PAL system COMBI Objects Note Figure 55 COMBI PAL Firmware Overview Page 2 OT PAL is a registered trademark of CTC Analytics AG Switzerland PAL COMBI x PAL LHX xt COMBI PAL LHX PAL User Manual pueyazyws Dy sonApeuy 312 jo xieursper parata e st Td 3jels jenpy LIS ANY ajeis jenpy 3jels apy LInO MS A enpy aui eounoqaq ales apy cu o do RP Eniy awi eounoqaq ales apy Installation and Operation 3Jejs jenby ales IAY I jenpy aes INDY ano 3jels jenpy IPS apy RP enpy aul aounoqaq 3jelS AY me ares envy u aw mowed Sau Pan 3jeis 9NDy Buuuny tue aes Jenpy ppu j wj 3 elS Jenpy aui eounoqaq awl Nd aui Suppojg 3 Pls JAPY JES 3NDy uoneurjsa 30Jn06S Palu anpqons Sjeusis 2u s
44. HX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation The reference position is the hole in the vial cap Teaching this position is identical for Wash or Waste vials 4 2 6 Injectors e g GC Inj1 Figure 35 GC Injectors e g GC Inj1 Reference Position For certain GC models centering rings are provided with the GC mounting kit If such a centering ring is available it should not only be used for teaching the injector position but also for routine work The ring stabilizes the needle guide of the PAL Injection Unit at the moment of needle penetration It acts as a guide tool and prevents needle bending For the GC Inj GC Inj2 the reference position is the appropriate GC Injection port The lower needle guide should be centered on the port with the bottom of the lower needle guide lightly touching its surface 4 2 7 Agitator Reference Position Figure 36 Agitator Reference Position The Agitator Reference Position is located as indicated in Figure 36 To define the position complete the following steps PAL is a registered trademark of CTC Analytics AG Switzerland note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 1 When the Job Queue menu is displayed complete the following sequence Menu Setup Objects Tray Holders Named Tray Holder 2 Select AgiHldr and scroll to Access Path Restore
45. IES SOE SGE sinz si z shiz 4 c C 0 0 mMOLAgO fot orgo Molaso WoL aso MOLAO piepueig piepuejs piepuejs TET if Loro iT Loro GM w 9M O jm 101 50 mMin c0 Win 60 MZZO miao if Lorzo i ot ir ot mol Slur 39 su 39 suk 55 SuisuodeA usn J Suuinjo pa pegd pio paluy Ald vomealuy uunjo uo vomaluy yds SjajauuPJeg POLAN Tyd Papuawwaray jauag lul apap poya qua8eay puepueysjuy 8njg IUINOS xap ypimpues TNS UD 15d IAS UD 35d ea lul is pa lul ag paads plui oy palui aq dning paads qi Ids up ad ZNS up ud LAS UD 24d IPNownjony s de5 uy BUNS PIS pjepuejsjur SUNOS AS nig AROS auinjoA ejduies 3ONPIAS FAD ipiwpues apo pouiaw ZHON eia ajdues ayy 021 0109 ul aunssaid Ysiy Aawa sdopa ap Js 007Z 0 payeay S 0 9 01 saem GOL N05 8 8 aanysiow urequo Aew ajdwes pijos Ag Sw SuurwApjs sjjWo0s 0st t fu 55 aunj P J UIeBe paydead s snjejs Apeay un SOl snipis peay wo 3 104 papaau awg awAUNy 5 ar a umumj zapy SAM ooz 01 00 Do OSL 03 SE saineiradiuia suoneoidde juasayip sc JO SOBULAS exa ast Jojejidy JO papayas se aunjejadua SC Jepus JO aues Ajjensp uongaridde uo spuadap anjeA ud osz aunjejaduis aduuAs TON jueuuidoja ap Jeo 007 01 SE poau yv pausijgeisa aq 0 seu au uoneqmut aur moog AWA uogeqnau NON SH SC 10 1 lul SH quanjos jo juod Suipioq japisuo UORed dde uo spuadap anjeA aunjexadw
46. Job Queue menu or the ESCape key to return to the previous menu 3 Method contents may be viewed from the Job Queue displays by selecting the desired Job pressing ENTER and the View Method function key 2 3 Delete Methods Methods can be deleted from the Methods menu Methods in use by an active Job cannot be deleted Complete the following menu selections to delete a Method Menu Methods Method name Del Method PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 Job and Job Queue A Job bundles the specified Tray with the designated vials samples and with the Method to run those samples Another term often used for Job in the chromatographic field is sequence If more than one Job is prepared the term Job Queue is used Before a Job can be activated the operator must verify that the Tray Type matches the specified Tray and vial size type This step is done in Utilities class Menu Utilities Tray select Tray Needle Penetr Tray Type Offset X Offset Y Scroll down 2009 05 06 16 07 20 Block Move to Home NdlG 001 Figure 3 Verifying Tray with corresponding Tray Type Select the corresponding Tray Type By activating function key F3 the injection unit can be moved to the first position and to another two corner positions to verify the correct selection of the Tray Type and teachi
47. L Firmware Overview 100 4 1 COMBI PAL Flow Chart based on PAL Firmware Version 2 5 X 100 4 2 PAL COMBI xt PAL System Flow Chart based on PAL Firmware Version 4 1 X 104 5 External Connectors for PAL and xt System 108 6 Intended Use for PAL System 112 6 1 Introduction 112 6 2 Intended Use 112 6 5 Disclaimer and Restrictions on Use 112 7 PAL Accessories 113 PAL is a registered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation C List of Figures Figure 1 PAL Control Terminal and Conventions Figure 2 Accessing a Method Screen Figure 3 Verifying Tray with corresponding Tray Type Figure 4 Example of Job Queue Screen Figure 5 Selecting Utilities Functions Figure 6 Selecting Info Functions Figure 7 Selecting Setup Functions Figure 8 COMBI PAL Major System Components Figure 9 Attachment of Mounting Claws Figure 10 Attaching the PAL Injection Unit Figure 11 Connecting the Injection Unit Ribbon Cable Figure 12 Inserting the Injection Unit Mounting Torx Screws Figure 13 COMBI PAL with Injection Unit and Standalone Supports Figure 14 Installing the Keypad Terminal Figure 15 Installing the Flush Gas Regulator Figure 16 Installing the Wash Station Figure 17 Installing the Agitator Figure 18 Installing a Tray Holder Figure 19 Electrical Connections for Combi PAL System Figure 20 Electrical Connections for PAL COMBI x System Figure 21 Obje
48. L xt systems operating with PAL Firmware version 4 1 X or higher APR Control board must be installed A PAL System can be upgraded to a PAL xfSystem for details contact your CTC Analytics representative For specific PAL hardware Modules a minimum PAL Firmware version Is required For example a PAL Dilutor Option requires the PAL Firmware version 2 3 X or higher and a PAL DLW Option requires the PAL Firmware version 4 1 X or higher Details are given either in the corresponding Addendum to PAL User Manual or in the Addendum to PAL User Manual for PAL Object Manager Software 1 7 2 Software Requirements The various PAL Software programs such as PAL Loader or PAL Object Manager are operated with Microsoft Windows Operating systems such as Windows XP and Vista For details see the corresponding Addendum to the PAL User Manual The PAL System can be controlled using PAL control software the Cycle Composer or any data system software that controls the PAL using the Cycle Editor for PAL ICC interpretation e g Analyst ChemStation Empower EZChrom MassLynx Xcalibur For details on software requirements for integration of the PAL System with other data handling systems CDS contact your CTC Analytics representative or the manufacturer s representative of the other CDS software program 1 8 Regulatory Compliance Requirements As of the date of publication this product is compliant with current RoHS and
49. LC MS System manufacturer A Do not turn the instrument on if you suspect that it has incurred any kind of electrical damage Instead disconnect the power cord and contact a CTC Analytics representative for a product evaluation Do not attempt to use the instrument until it has been evaluated Electrical damage may have occurred if the PAL System shows visible signs of damage exposure to any liquids or has been transported under severe stress A Damage can also result if the instrument is stored for prolonged periods under unfavorable conditions e g subjected to heat water etc Ensure that the power supply controller unit is always placed on a dry and clean position Avoid any spill of liquids A Always disconnect the power cord s from the power supply or from the various power supplies if optional devices are installed before attempting any type of maintenance Capacitors inside the instrument may still be charged even if the instrument Is turned off To avoid damaging electrical parts do not disconnect an electrical assembly while power is applied to the PAL system Once the power is turned off wait approximately 50 seconds before you disconnect an assembly PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation The instrument includes a number of integrated circuits These circuits may be damaged if expos
50. Lfuj55 ate Scag SSE morgo EE Moroso Morao pepui pepum pipues Mro iio MoO MMM mM 10 50 ji 0160 i 0160 o qeoco0 3 qmiocO0 ZO mol mor mol Slur 55 slur 55 Slur 25 SutsuodeA usn j pio paluy Ald SUWUN O paxpeg uomafuj uunjo uO uorpalu yds SjojatueJeg PON Tyd papuauuuio ay 3umumy 25 inoayeg 9q aul poseg IVd PIA dwa equ awi IU 34d JONIYAS apa pouyyayy qua8gay paepuelsiul BNjq IUOS ap upiwpues ZNS UD Id LAS Up Sd jg fur Sq eq lup aig paads pafu 0 patu pa dnjng paads i4 ds u ad TNS UD 9d IAS UD Ad IPNawnjoniy s den any SUNOS PIS PUPPUP SIU a0unos AS SN d JuaA 0G 3ulnjoA 3 a wes JONIYAS EDO tpiwpues ap poujaw TION ul aunssaud y y jaurazixa sdojanap gt 007 0 payeay S O 010l IDEM 8601 IOS 8 3 aunysiow urejuo Aew ajdwes pijos sul 0 SBUIEM LS sAN 006 OSZ Liu 55 aui ap uie8e paypea si smpejs Apeay nun Sor oio umumjj5 zsow SIT ooz ooo Jo OSL 0 SE saumperaduiay suoneoiidde jusiayip sc 10 SOBUUAS PIXE as OSY 20 papajasseanjeiedwa SG Jepus Jo ates Ajpens uoneardde uo spuadap anea 7d geez anejaduja a8uu s DEN juaudojanap Do 007 9 SE poujaui uw Paysiiqeysa aq 0 seu aui uoneqnout ayy moos auin uoneqnau RON SH C 20 OL lul SH Jua jos jo 1utod Suijoq apisuo uone rjdde uo spuadap anjeA 3njejaduia uoneqnou SjajatuPJed POW Td papuauluoJ9y
51. MBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation Requirements 648 mm 25 5 in 385 mm 14 1 in 828 mm 32 6 in 10 kg 22 lbs without accessories 1 5 Operating and Environmental Requirements Parameter Operating temperature range Maximum relative humidity Bench space Vibration Static electricity 1 6 Sound Pressure Level Parameter Sound Pressure Level Requirements 4 to 40 C 39 to 104 F 75 non condensing At least 16 cm 6 in at the rear Access to power switch es and power cord s Clean level and smooth surface Solid bench plate Negligible Negligible Requirements Measured value 62 dBA PAL System used for measurement One meter from the equipment in the direction of maximum sound pressure level According to UL 610107A 1 1 Edition clause 12 5 Limit lt 85 dBA dBA A weighted sound pressure level 5 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 1 7 Hardware and Software Requirements 1 7 1 Hardware Requirements In order to operate a PAL System the electronic control board must be compatible with the corresponding PAL System PAL System operated with PAL Firmware version 2 X X or 3 0 X either the combination of APR CPU and APR MOTIO or the APR Control boards can be used PA
52. Maintenance Step Interval Use only a soft lint free cloth dampened with mild Weekly or as needed soap and water Clean Instrument syringe and surfaces Weekly or as needed Replace the syringe plunger Syringe Plunger for a liquid syringe Metal Plunger sealing against glass should be cleaned on a regular basis with an aqueous alcohol solution Be aware that this plunger type should not be used for samples with a high amount of matrices for clinical or residue analysis A polymer plunger tip would be more suitable Syringe Plunger for a gas tight syringe polymer tip has to be replaced on a regular basis The interval is highly dependent on the application throughput quality of sample solution particles etc washing solvent It is advisable to check the tightness of the plunger on a regular basis to gain experience with the application Wash and Waste Solvent reservoirs Weekly or as needed Replace the Septum as well to avoid carry over The other steps are described in the Preventative Maintenance Procedure Specific kits for the GC or the HPLC techniques are available The kits include the parts which must be changed annually e PM Kitl for HPLC technique e PM Kit2 for GC technique Contact your CTC Analytics representative to perform an annual preventative maintenance on the PAL System There are no operator serviceable or replaceable parts inside the power supply ies or the PAL System In case of a failu
53. Motor Z For the Motor Z following speeds are selectable Penetr Speed This is the speed used to penetrate the syringe needle into any object with the exception of the injector Example Vial penetration Inj Penet Spd Injector Penetration Speed With PAL Firmware version 2 X and 3 0 the injector penetration speed is identical to the injector retraction speed Inj Retr Spd Injector Retract Speed With PAL Firmware version 4 1 X the injector speed was separated into a penetration and a retraction speed This allows safe penetration of a vial by any type and diameter of syringe needle without bending through the septum of the GC injector The needle retraction speed can be increased to speed up the entire injection process This new feature enables the user to avoid uncontrolled evaporation of any remaining sample solution in the needle The visibility of the Motor Z parameters at the Extended User Level was introduced with PAL Firmware version 4 1 3 Items displayed in italic are available beginning at PAL Firmware Version 4 1 X Items displayed in bold are explained in detail below 74 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 9 3 1 Tray Type The following object Tray Type Items may be changed by selecting the particular item First select the desired Tray Type such as V
54. PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation Motors In the class Motors the Objects Motor Y and Motor Z are accessible to enable the user to select specific motor parameters in case a specific application requires an adjustment Motor Y Strategy Three different settings are selectable for the Y Motor movement strategy Rectang normal movement as used for PAL system On firmware version 4 01X this mode was called Auto AY Simul X Y axes move simultaneously which allows movement in a diagonal direction At firmware version lt 4 1 X this mode was called Rectang but it was not active at lower versions Retract Y axis moves from any point first to zero Y back to the x axis before the X movement is started This mode allows bypassing an object such as a large GC detector which is in the travel path if the mode Rectang Is used Path Speed Y axis motor speed in conjunction with a Path example opening and closing a drawer of a Stack In certain situations it is necessary to slow down the speed to open and close a drawer Typical application example is fraction collection collecting directly into a well plate If the liquid level is high the liquid can wash over into the next row if drawer movement is too fast Remark At PAL Firmware version 4 1 X these items of the Y motor have been made available at the Extended User Level
55. PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation A dialog window opens displaying a note to Adjust offset X Y Z for position 001 Continuing in this dialog will provide the user the opportunity to adjust all three axes perfectly by the top of the vial Please note that this step is only valid for position number 001 Adjust Offset X Y Z for Position Figure 29 Adjusting Offset X Y Z for Tray Position 001 To check the next corner of the Tray activate function key F3 again example Tray Type VT32 position 008 The next information provided by the pop up window asks to correct a possible inclination of the Row Continuing the dialog provides the user the opportunity to adjust the three axes perfectly to match the vial top or in case of a well plate the top of the plate FS Move to 008 Adjust dx dy dzRow for Position Figure 30 Adjusting Row Inclination at second Tray corner position The last step will be to move to the next corner of the Tray by again activating function key F2 This will be position 32 in the example of Tray Type VT32 A possible inclination of the Tray column can be corrected in the same manner as described above PAL is a registered trademark of CTC Analytics AG Switzerland note note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation F3 Move to 032 Adjust dx dy
56. Path 5 If the Agitator is located on the left side choose AgiOpnL AgiClsL If the Agitator is located on the right side choose AgiOpnR AgiCIsR Steps 2 3 are obsolete beginning with Firmware version 2 2 0 or higher 4 Open the white Agitator cover manually and block it open with a screwdriver fitting it into the side of the cover 5 Insert a 20 mL vial in sample position No 1 6 Attach the red vial transport ring to the lower needle guide 7 Define the X Y Z positions common to all objects as described in topic 4 1 The reference position is located at the hole in the steel vial cap The lower needle guide should be centered on the vial cap and should touch the cap 8 Remove the screwdriver and close the Agitator cover After all positions have been set perform a firmware backup of the existing configuration by using the PAL Loader software For instructions see below 55 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation 5 Syringes 5 1 Selecting Syringes The standard COMBI PAL is shipped with two syringe kits One PAL SyrGC10uL Kit GC Syringe 10 uL for COMBI PAL consisting of 1 pc syringe adapter 1 pc plunger holder 2 pcs syringe SYRC L10 26S AS or 2 pcs syringes SYRC L10 23S AS in combination with Shimadzu GC 1 PAL SyrHS2 5mL Kit HS Syringe 2 5 mL for COMBI PAL co
57. Slv Source Wash Stations Vials Overfill aspirate air outside of vial if Air Gap s Needle Penetr Air Gap s 0 Fill Speed Pullup Del 8 Fill syringe with IntStd from IntStd SYRINGE Syringes Fill Speed Std Source aspirate air Std Source Wash Stations Vials Overfill outside of vial ifairgap S Air Gap s Needle Penetr 0 Fill Speed Pullup Del 9 Fill syringe with sample Sample SYRINGE Syringes Fill Speed volume aspirate air outside Volume TRAY Tray Type Overfill of vial if AirVolumeNdl 2 0 AirVolumeNdl Needle Penetr Fill Speed Pullup Del 10 Watt for Inject signal Inject Sync Signals 11 Move to Injector Inject to 12 Drive needle to penetration Inject to Injectors Needle Penetr depth MotorZ Motors Inj Penet Spd 13 Delay Pre Inj Del 14 Activate Injected signal Injected Out Signals Pulse Time 14 Activate Injectd signal Injectd Out Signals Pulse Time 15 Dispense syringe contents Inject Speed into GC Injector 16 X Delay Pst Inj Del 17 Retract needle from injector MotorZ Motors Inj Penet Spd 18 Clean syringe with Pst Cln Slv1 SYRINGE Syringes Fill Speed solvent from Wash1 Eject Speed selected number of times Washi Wash Needle Penetr Stations Clean Volume Needle Penetr Waste Injectors 19 Clean syringe with Pst Cln Slv2 SYRINGE Syringes Fill Speed solvent from Wash2 Eject Speed selected number of times Wash2 Wash Needle Penetr Stations Clean Volume Waste2 Injectors Needle Pe
58. T32 20 The definition of Row and Column ts given above in Section F Description and Installation point 4 2 2 1 By reversing the first six entries the order of injection can be changed Example Tray Type VT32 20 has a Row Length Y and a Col Length X defined Reversing to Row Length X and Col Length Y would start with sampling from left to right as used e g with Tray Type DW96 The values of the items Spl Per Row and Spl Per Col must also be reversed Item Description Row Length X Defines the Row length in the X direction from the center of the first to the center of the last vial position measured in mm Row Length Y Defines the Row length in the Y direction from the center of the first to the center of the last vial position measured in mm Col Length X Defines the Column length in the X direction from the center of the first to the center of the last vial position measured in mm Col Length Y Defines the Column length in the Y direction from the center of the first to the center of the last vial position measured in mm Spl Per Row Number of samples per Row Spl Per Col Number of samples per Column Pattern The pattern of the Row or Column arrangement has to be defined Regular square arrangement standard Stagerd Rows are arranged in staggered pattern Offset of second Row Is shifted by 50 of hole pattern Staggrd Rows are arranged in staggered pattern Offset of second Row I
59. The following tool items may be changed by selecting the particular item Item Description Teach Point The reference point at which to teach the Object Tool MHETool is selectable In the case of the MHETool no extra position is necessary The Parking Station is the fix point Select None ToolOffset X If necessary a correction to the ideal X position of the MHETool can be made by using ToolOffsetXX ToolOffset Y If necessary a correction to the ideal Y position of the MHETool can be made by using ToolOffsetY ToolOffset 7 If necessary a correction to the ideal Z position of the MHETool can be made by using ToolOffsetZ A ToolOffsetZ of 48 0 mm is necessary to position the MHETool on the sample vial 5 Logfile The PAL logfile cannot be read out directly on the terminal display However the function key F3 does allow a print out A serial printer has to be connected to port SER2 for the PAL System and to the port SERT for PAL Xt System If a serial printer is not available use a serial parallel converter 24 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 6 Info Functions Info functions selectable from the Menu screen provide quick access to information to be viewed These info functions are available for the Hardware Software Maintenance Free Objects and Free Items All items with
60. Tools is used to handle another device such as the MHE Tool Multiple Headspace Extraction Tool or MALDI Tool For details see the specific Addendum for the PAL User Manual The various positions used with the PAL System are selectable at this level Positions H i such as Home Change Syringe or a Path Point reference the Paths for a specified Tray Holder directly such as Agitator or Stack Selecting an item e g Home will provide the function key F1 Check Pos on the screen Activating this function provides the possibility of verifying X Y Z axes values for this particular position A Path defines specific movements for the Injection Unit which represent a repetitive task assigned to a Tray Holder Typical examples are opening and dosing a drawer from a Stack or opening the lid of an Agitator The Path is composed of several steps points which are added up in a sequence Each individual step point can be positioned by teaching X Y Z axes In most cases the return path is in the reverse order of the starting path Example AgiOpnL and AgiCIsL If one setting of a point is changed this change will be valid for the return path as well The various synchronization signals are described above in Section F Description and Installation point 6 1 At this level no other functionality as shown at the user level is provided The various Out Signals are described above in Section F Description and Instal
61. X as shown in the flow chart corresponds to the PAL System The changes from Firmware version 2 5 X as used for the PAL are emphasized in this PAL User Manual note System Z SHO 1001 A IPSHO 00 X 195JO J001 lutog Yee 00 JHW ee iea pal paads pal paads aq dnjjng auinjoA aulld JAS 10njiQ Sod inii J S 38uuAs JjoUad 3 paaN OU Tuem saUAd a paaN tusem uonels YSE dwa jenpy dwaj qpuejs Jj9uag 9 pa3N J9 amp 2H PN Jjouad a paaN 055 sein dull JO By aul uo I8 paads duia jenpy dwa Aqpueys Io zp 0o Ap ODxp moyzp MOyAp MOYXP Z SYO A PSHO X 19540 adA Aeij Hud 3 pa3N __ Ud 3 paaN LABJ naw M IAJ AQ MUIA JX IgIN OO nasas Jud Sog qpuels Sog BU Sid paads palu paads pal paads I4 eq dnyjng SOYONS I 9UInjoA al enpy _ Sog BUYJ 8 duia jenpy dwa qpuejs paads pal paads pal3 paads eq dnjng SOYONS 4 WNJOA I jenpy adedspeay SHWS Z Sog a8uy Bid paads palu paads pal paeds I4 eq dnd SOYONS I 4 AWNIOA I4 al jenpy pinbr riot 6007 aunf X o aem H UOISINaY WA SAS T d enpipur ipea 30 uonein8ijuo asempsey au uo spuadap okej au MalAJaNo ati ur uwous se palqo fusa apnpul jou Saop aJe jos pepuejs aug auinunm jnoayeg J9qi 3ull qJosa quad lul 0 qosaq awl Pex Jj9uad BIA aui HO Iv aul uo I3y paads i8y dw
62. YO 1001 ASHO L001 XSYO JOO juog pea 7 Sog Suhyieg A Sod Sued X sod Sued 00L JHW Figure 60 PAL COMBI xt Firmware Overview page 3 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt User Manual PA system Installation and Operation HAQWOJANQKULY Tyd ON Hed smeys Apeas O Appas woy aw 3A 99 uado Suiueap 104 pasn aq ipw UONEIS Suruoqipuo jaqi4 0 lt SI BWN ji 0 Suiueap saqu 30 awn JASUO Sul ui uongiosap Pul3u uiu 0 Z 1opa ur jo adA uo spuada WW Gp 0 S O llu35 uonqiospe qe jusuidoja ap poau juapuadap uonexiddy uado AjguipJO22 jei ui auunjo ajdues jsn py WW Le 0 ZZ awg pea pue ssa2o1d uoneqrour aJd JOY atum WO sc aui pexa pue ssaooid uoneqrbul 3id JOY atu uc SG Auo ssadoid uoneqrouaud jo pads wdi OGL COST Mpeg pened DU SE pauasul si aqy a10jaq ajduies ayeaquinba 0 au uonePqmour aig SANUIW LIIAS 01 Q Aq IWdS SUPLA pouiaw Tvd SYLWJY Papuawworay Jopalut joy ui snoauezuods spine iueo yo uoneaijuams3 ajdwexy uonnjos ajdwies auo Woy jou Ajayeedas aBuUAs aui OJU umeJp 3q I piepueis jeujajur uongis USE WAY pJepuels uonsog 833 uonnjos JUaSeay Jo pyepuels JPuJalu JO JerA BINS peres UONEIS YSEA LOY YSEM uonisog 8a Je INOS Papas BULAS puepuejs Trl OL e ym Tri g i gt sauinjoA ajduues plur o pasn Aure 0 0 0 Soc 01 score stu 0 su 0 sim oc c sin c 1 Lluj55
63. YSTEM User Manual Installation and Operation Connector AUXI AUX2 PAL and xt System 20 11 Pin Signal Name 1 2 Motor Al Wil Motor A2 Za Motor B1 15 14 Motor B2 15 18 19 GND 6 Temp 16 Temp y Sens 17 45V 8 9 Heater 20 10 56V Connector Wash Station PAL x System 2 PWR Out 1 3 PWR Out 2 1 PWR Out 1 4 PWR Out 2 Pin Signal Name 1 PWR Outl 2 PWR Outl 3 PWR Out2 4 PWR Out2 Connector Power PAL and xfSystem 1 36V 3 NC 2 GND Pin Signal Name 36 V 2 GND 3 NC 110 PAL is a registered trademark of CTC Analytics AG Switzerland system COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation Connector MODBUS PAL xfSystem Pin Signal Name 1 TXD D 36 V 8 RXD 9 14 GND Connector LAN PAL x System LED left LED right el 8 1 Pin Signal Name DIR TX Out 2 TX Out 3 RX In 45 Not used 6 RX In 7 8 Not used Connector Fuse PAL xf System Fuse Type FST 5x20 Rating 6 5A Rated Voltage 250 VAC PAL is a registered trademark of CTC Analytics AG Switzerland Primary Function Transmit Data Transmit Data Receive Data Terminated Receive Data Terminated 111 COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 6 Intended Use for PAL System 6 1 Introduction The brand name PAL System represents an entire product fami
64. a particular method should also be installed Figure 1 illustrates the PAL control terminal and the conventions used to enter edit and view information JOB QUEUE ACTIVE Menu Title Job 03 Stkl 01 001 096 Curser bar highlights an item in list 04 2mkl 001 O13 Stk1 01 Spl 001 8 09 7 Function Key Definitions Function Keys Press STOP to abort operation Press the inner knob to select an item highlighted by the curser bar Press again to ENTER the selection Rotate the outer knob to move the curser bar Press ESCape to return to the previous level Figure 1 PAL Control Terminal and Conventions 1 1 Menu Screens Different menu screens are displayed depending on the COMBI PAL operating state and the particular function being accessed by the operator All menu screens have the same basic format The menu title is displayed at the top of the screen A list of items is displayed below the title The date and time or status are shown in the highlighted area above the Function key labels on the bottom of the screen PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Menu Methods LC 100 Scroll down Figure 2 Accessing a Method Screen 1 2 Function Keys Options for a particular menu are assigned to the corresponding function keys F1 F2 F3 and F4 directly below each function key label
65. aj yeqmu OUI IU Ald 3ONIMAS DAD IWdS apAd 55 1 Qi suorpefu JuaJayIp OML 3ullgum BUIYSN 4 JAS fad lur 1g eq lul aud paads p luj 0 palu eq dnjng paads i4 duia a8uuAs aul JO Isy alu UC IBY paads sy 3uli jeqnou dway yeqrou auunjoA ajdwes JONIYAS IDA a ueusjurejw ojuj nuayi suno uoimalu pue aXons JaBuN d eng 39 SpA e qepojes IO ZNS UD Sd LAIS UD bd pa lur isa TNS UPD 18d pq lul aud LAIS U 18d paads p lul pa fusd 0 palu aq lul ag a dnyjng paads p luj paads I 0 palu TNS UD ald pa dying LAS UD ald S2011S IpNauunjofuly paads J s de sry WUNJOA J 90Jn0S PIS Ids up 34d PISUI CNS UD Ad DINOS NS LAS UD 9Jd 8n g 1u2A0S 9unjoA JI auinjoA a duues auunjoA a duues 3ONIMAS 3ONISAS DAD IDA SPOR SIJON Figure 58 PAL COMBI xt Firmware Overview Page 1 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL 6007 aun X 0 p emus H UOISIN WA SAS Ty d jenpiupul upea JO uoneinsijuoo aJeMpaeu ay uo spuadap 1noAe dy Mal Ja o al ul uMous se palqo Ayana apnpur jou saop ILMYOS pJepueis ay 9 ON PAL COMBI x PAL LHX xt User Manual Installation and Operation J9pJ0H3 0 3JseAA 3 auinjoA aui J3yeaH DN ApIHieV aulnjoA mi cM 0CCt S1 Aeq pal poads pal C amn ae IM wn LJ9 00 U9 0051 pyepuejs pds pal xew u 55 U ol Pu L Peis JaUad 3 paaN pds iJ xew Zan 5 pds pala xew
66. and the Job Queue Menu is displayed Available Vial types Standard Vial in Standard Washstation for GC front position Fiber Exp Position to block needle guide Mainly used for SPME application See SPME User Manual Item Description Needle Penetr By selecting the parameter Needle Penetr the wash station needle penetration value can be checked and or changed 4 6 Dilutors After selecting the specific Dilutor the following functions are available Function Key Description F1 Prime The Dilutor syringe is primed with solvent after activating the F1 function F2 Chang DSyr Activating F2 moves the plunger of the Dilutor Syringe to standby position to allow easy access to change the syringe F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Dilutor items can be changed by selecting the particular item Item Description Syringe Indicates the Dilutor side port syringe inserted in the Z axis This function allows selecting another syringe size Syr Dilut Pos Activating this function moves the plunger of the side port syringe up by the specified distance This allows adjustment of the plunger tip of the side port syringe exactly above the lower side port solvent inlet This fine tuning is necessary after changing a side port syringe to allow unrestricted solvent flow Dilutor Syr Indicates Dilutor Syringe is installed If the syringe size is changed this item must be a
67. bove How can you get Technical Assistance To obtain technical assistance call the responsible CTC Analytics representative from whom the instrument was originally purchased Please be prepared to provide the serial number of the instrument and discuss the problem in detail and be willing to perform recommended tests and adjustments to help us determine the probable cause of and solution to the problem What must you do to keep the Warranty in Effect To keep this warranty in effect the user must take care to avoid accidents misuse and abuse as described above under What is Not covered by this Warranty The maintenance guidelines outlined in the PAL User Manual must be followed Preventative Maintenance PM or any service or repair task on the instrument must be carried out by CTC Analytics authorized personnel only Normal Responsibilities of the Buyer The user must report any problem with the equipment to the responsible representative of CTC Analytics AG and assist the technical support representative in diagnosing and attempting to resolve the malfunction of the instrument It is expected that the user will cooperate and will install any replacement parts modules that have been sent and will return any exchanges or unused replacement parts to the responsible representative of CTC Analytics AG at previously agreed conditions How does the LAW of various Countries or States relate to this Warranty This warranty gives you
68. bpeis 7 UONISOd paads uly Pds 4 Xe VO LIS OPIHI A UOf iSOg vr 9 P2S Jjouag IPN Ijouad 3 paoN 0 10 LIS X UOl SOg S JoInjiq Z UONISOg Z UONISOg Z UONISOg sod 1njig JAS A uonisog A UONISOg ven A UONISOg dx aqy a8uu S X UONISOg X uonISOd ad Aes X UOIISOg I auag APN Joni tur 55 WweMrl Wer UpIHL SIRIA sona suopaluy suoners usw SAR siapjoH Ae se mo spefqo 2X I8WOD nass Td system Figure 59 PAL COMBI x Firmware Overview Page 2 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI x PAL LHX xt User Manual Installation and Operation system puepiazyms Dy son amp puy 34 jo xieuiape paars al e SI TW 3jels jenpy 3jels INNIY xog dx3 INO SANJA Jeuas RAS jenpy 3jgis aupy ano MS agis jenpy RBS apy LINO MS RAS jenpy aui aunogag aes Apy zuodo jenpy aui aunogag aS Apy 3jeis jenpy 3S aupy ano apis jenpy IBIS apy ano 2415 jenpy aeS aupy 20S jenpy aui aounoqa aes aw py u 1L DPS jenpy aui aounoqag BPS aou py cur a5 jenpy aui exunoqa ajeis apy su Buluuny Buluuny ep palu all asin uoneunsaq C popar S PUBIS INO ms aui Sunpojg anos anoqons esus 899 J auag 00 Z S
69. c characters and spaces Press the ENTER function key F4 to accept the name To create a new Method complete the following steps Menu Methods Select the insertion point Insrt Method 1 Assign and enter a new Method name as above After a copy of the Method has been created the Method parameters will display and can be edited The Cycle and Syringe entries cannot be changed 2 Ifthe Method is new i e added select and enter a Cycle that is appropriate for the application 3 Select the specific Syringe to be used by the Methoa Once a Method has been created and saved the Cycle and Syringe cannot be changed To use a different Cycle or Syringe a new Method must be created 4 Assign Parameter values according to the application requirements Consult Appendix point 3 COMBI PAL Injection Cycle Parameters for details on specific items PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 2 2 Edit View Methods Method parameters excluding Cycle and Syringe can be viewed and changed from the Method menu as follows Complete the following menu selections Menu Methods Method name Enter 1 Scroll to and select the parameter to be changed Assign the new value and press the ENTER key 2 Exit from Parameter List by pressing either the Home function key F4 to return to the top level
70. cal level such as an electrical wall outlet A Use only fuses of the type and current rating specified Do not use repaired fuses and do not short circuit the fuse holder The supplied power cord must be inserted into a power outlet with a protective earth contact ground When using an extension cord make sure that the cord also has an earth contact PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Do not change the external or internal grounding connections Tampering with or disconnecting these connections could endanger you and or damage the PAL System The instrument is properly grounded in accordance with these regulations when shipped You do not need to make any changes to the electrical connections or the instrument s chassis to ensure safe operation A The combination of a PAL System with a LC MS System does require the safety measure as described by the LC MS System manufacturer Detailed instructions for the safety grounding on the LC MS system are outlined in the corresponding operating installation manual CTC Analytics recommends using a grounding cable connected on one side at the Injection Valve Loop or any other suitable direct metallic contact and on the other side at an appropriate ground point on the LC MS System This supplementary grounding measure will support the safety strategy of the
71. cs AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 2 4 Wash Station Waste Waste2 Reference Point Injectors Waste Waste2 The Waste positions represent an Injector within the PAL software It is defined in the Object class Injectors For Waste Waste2 the reference positions are the two holes in the wash vial caps see Fig 33 The lower needle guide should be centered in these holes with the bottom of the lower needle guide lightly touching the surface of the caps Waste 2 Waste ae Wash 1 um Wash 2 C 2 C j Waste D Waste 2 pe Standard Figure 33 Wash Station Waste Waste2 Reference Position 4 2 5 Vials e g Standard Position in Wash Station axl 4 cg ZL SZ pz Standard L Wash 2 C J ZZ Waste OE A p Figure 34 Wash Station Standard Reference Position NUN The position Standard on the Standard GC Wash Station is defined in the PAL Firmware class Vials This position can be used for a standard an internal standard a reagent solution a calibrator solution or any other type of solution The advantage to the user is that a large quantity of solution is available and can be defined by Job conventionally known as Sample List or Sequence in other systems 5 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL L
72. ct Reference Position Figure 22 Menu Screen Object Tray Holder Figure 25 Inclined Tray Corrections for X Y Z Axes Figure 24 Tray Holder Reference Position Figure 25 Demonstrating a possible Inclination of Tray in X Y Z Axes Figure 26 Tray Type VT 22 Figure 27 Tray Type DW96 Figure 28 Utilities Function Trays Figure 29 Adjusting Offset X Y Z for Tray Position 001 Figure 30 Adjusting Row Inclination at Second Tray Corner Position Figure 31 Adjusting Column Inclination at Third Tray Corner Position Figure 32 Wash Station Wash1 Wash2 Reference Point Figure 33 Wash Station Waste Waste2 Reference Position Figure 34 Wash Station Standard Reference Position Figure 35 GC Injectors e g GC Inj1 Reference Position Figure 36 Agitator Reference Position Figure 37 Syringe and Syringe Adapter for Liquid Injections Figure 38 Installing and Removing a Syringe for Liquid Injections Figure 39 Headspace Syringe and heated Syringe Adapter Figure 40 Installing and Removing a Headspace Syringe Figure 41 Removing a Headspace Syringe from the Heated Syringe Adapter Figure 42 COM Port Settings Figure 43 Reading MAC Address from Ethernet Communication Port Figure 44 PAL xXt Communication Settings Figure 45 Setting Communication Mode PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation List of Figures contd Figure 46 Figure
73. dapted accordingly The syringe specific dimensions are coordinated by this function Prime Volume A volume to prime the Dilutor Syringe can be specified The allowed range can be fourfold higher than the actual syringe volume This allows filling and emptying a dilutor syringe more often than just once Pullup Delay Using this item permits selecting a delay time between solvent filling and ejection while filling or priming the syringe Fill Speed Speed of plunger movement of Dilutor Syringe used in all syringe filling operations Eject Speed Speed of Dilutor Syringe plunger movement used in all dilutor syringe eject operations Eject Delay Using this item permits selecting a delay time between solvent ejection and filling while filling or priming the syringe 25 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 7 Tools The Object class Tools is available with PAL Firmware version 3 0 X 4 1 X or higher After selecting the specific tool the following functions become available Function Key Description F1 Chk Offs The item Check Offset can be used to verify the offset from the syringe needle tip to the tool tip This item is mainly used for the MALDI tool it is not active for the MHE tool F4 HOME The injection unit moves to its HOME position and the Job Queue Menu is displayed
74. ddress is stored in the system firmware and can be viewed on the terminal as follows Communication Scroll down ud T 07 20 Set Home Comm i Figure 44 PAL Xt Communication Settings H i Pressing function key F1 Set Comm sets the selected communication mode The same is achieved by pressing ESC or Home PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Communication parameters are written please wait 2009 04 17 16 07 20 Set Set Home Comm Deflt Figure 45 Setting Communication Mode E PAL is a registered trademark of CTC Analytics AG Switzerland system 8 2 1 Explanations for the various settings for the Communication Protocol Parameter Commbort DHCP IPAddr1 to 4 SubNet to 4 TCPPort StdGateway 1 to 4 MACAddr1 to6 Explanation Setup for serial SER1 or LAN communication Dynamic Host Configuration Protocol If set to Off the IP address is provided by the user see IPAddr1 to 4 The user can ask the network administrator for a suitable IP address If set to On an IP address is dynamically sought throughout the provided network Enter the IP address divided into four blocks example IP default Address 192 168 0 1 SubNet mask for network configuration This parameter is read only no
75. e for GC Immediat Dual cycle Ignore Injected Out Signal Activated at the moment the SW Outl 3 sample has been injected 4 Injectd2 Out Signal Activated at the moment the Off second sample has been injected Running Out Signal Active as long as the Job Queue is Off being processed and the PAL is not in an error state 1 If used to synchronize with Sync cable change setting to TTL In1 I If an item of the class Sync Signal is assigned to an output signal such as TTL In1 then this TTL contact must be defined as either Active High or Active Low as expected by the GC system This is defined in the PAL Firmware class Events 59 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Menu Setup Objects Events Select the corresponding item and set the mode accordingly The setting is provided with the schematic description of the GC Sync Cable supplied with the specific GC Mounting Kit 7 PAL System Software 7 1 PAL Loader Software The purpose of PAL Loader Software is to create a backup file of the complete PAL System Firmware The core software firmware and all PAL Firmware Objects including all settings Items the local methods and jobs are saved in one file The PAL Loader software has a second function of loading the firmware or a complete backup file to a
76. ections Safety Information COMBI PAL Operating Instructions Specifications Product Warranty COMBI PAL Description and Installation Troubleshooting Replacing Parts Maintaining PAL System Appendices The COMBI PAL Operating Instructions in section E are intended for infrequent PAL users or new users who already have experience using automated systems to perform standard analytical methods The COMBI PAL must be installed and set up properly before the Operating Instructions in Section E can be used Users who are installing a COMBI PAL system or COMBI PAL accessories or who need to adjust an installed system should consult COMBI PAL Description and Installation in Section F The Appendices provide useful information such as the Software Flow Chart Definition of Terms Injection Cycle parameter descriptions or the COMBI PAL accessories guide PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation E COMBI PAL Operating Instructions 1 Using the Control Terminal The following procedures present the key steps required to set up and process multiple groups of samples with the COMBI PAL They are intended to provide an overview for new users and a reminder for infrequent users The COMBI PAL and all accessories should be installed with Objects defined correctly A syringe of the specific type called for by
77. ed Tray including the sample vials must be present After pressing Movto 001 the Injection Unit moves to sample position no 1 This procedure can be repeated for the last sample position in the first row and the last sample position The Injection Unit moves to its HOME position and the Job Queue Menu is displayed Available for Agitator only The following Tray items may be changed by selecting the particular item Description Needle penetration depth into the sample vial The needle penetration depth for the selected Tray may be changed by entering the desired value Tray Type The Tray Type which is selected for the Tray is shown If the Tray enables the use of different Tray Types it can be changed at this position Tray Offset X If necessary a correction to the ideal X position of Position 1 can be made by using Tray Offset X Tray Offset Y If necessary a correction to the ideal Y position of Position 1 can be made by using Tray Offset Y Tray Offset Z If necessary a correction to the ideal Z position of Position 1 can be made by using Tray Offset Z dxRow Correction of any inclination of a Tray plate in the X axis of a row see Fig 25 for details dyROW Correction of any inclination of a Tray plate in the Y axis of a row see Fig 25 for details dzRow Correction of any inclination of a Tray plate in the Z axis of a row see Fig 25 for details dxCol Correction of any inclination of a Tray plate
78. ed Tray Holder e g THldr1 Tray Holder THlarl Position X 63 2mm Position Y 178 5mm Position Z 188 4mm Access Path None 2009 05 25 11 20 25 Check Clear Movto Home Pos Pos Pos Figure 22 Menu Screen Object Tray Holder 4 After selecting Named Tray Holder the X Y Z positions associated with the object will be displayed see Fig 22 5 Highlight item Position X with the cursor bar and press ENTER The Injection Unit will move to the previously defined X axis position 6 Rotate the outer knob to adjust the X axis position to the Tray Holder reference position see Fig 21 7 Press the inner knob to ENTER the position X value 8 Repeat steps 5 7 for Position Y and Position Z 9 If the F3 button Moveto Zero is activated the Injection Unit will move to the HOME position 10 Verify the defined X Y Z positions by pressing F1 Check Pos note PAL Firmware verson 3 0 X permits correcting for an inclined surface for example a Tray Holder or Tray where the Z axis position in front differs from the rear position For applications such as the PAL MALDI Option perfect alignment of the MALDI tip is vital This compensation for the Z axis position Is provided in the firmware class Tool 44 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation For the device PAL MHE Too
79. ed to excessive line voltage fluctuations and or power surges A Never try to repair or replace any components of the instrument that are not described in this manual without the assistance of a CTC Analytics representative There are no operator serviceable or replaceable parts inside the power supply ies or in the PAL System If a power supply is not functioning contact a CTC Analytics representative A The power supplies for the PAL Instrument the Stack Cooler DW Stack Cooler MT and Tray Cooler have the symbols 1 0 on the label for the power switch to switch ON OFF Any additional power supply for other devices such as a Valve Module exhibits the symbols as shown below on the label for the power switch O Power ON Power OFF The symbols warn the user that in an emergency more than one power supply must be turned OFF or more than one power cord must be pulled from the power supply or from the wall outlet to shut down the complete PAL System If the basic PAL System Is installed then a single power supply only is installed Turning OFF the power supply or pulling this single power cord will stop the complete PAL System in an emergency If a Stack Cooler DW Stack Cooler MT or a Tray Cooler is installed in combination with a PAL System then a second power supply is installed in the complete system Turning OFF the power supplies or pulling the two power cords in an emergency will stop the complete PAL System It is
80. eded In the Object Tray Types details for the various Items are described more closely Tray Types The object dass Tray Type is only accessible via the PAL Extended User Level All loaded Tray Types are visible and selectable Details see below Tray Holders The Firmware Objects accessed on the PAL Extended User Level have more specific items selectable than at the User level Depending on the type of Tray Holder e g an Access and Restore Path or a Heater and Agitator Motor is assigned Typical example the Tray Holder Agitator Trays All loaded Trays are visible and accessible A Tray must be assigned to a Tray Holder A TrayTypeGroup identification checks if the specified Tray Type is allowed in this combination The offsets X Y Z and the inclination corrections for a row or column are explained in the Utilities Functions section The Path Offset X Y Z is used only if a Path is assigned to the specified Tray Holder typically a Stack or Agitator uses a Path Wash Stations The Wash station and its positions Wash1 and Wash2 are visible and selectable Details see below Injectors The Firmware Objects accessed on the PAL Extended User Mode have more specific items selectable than at the User level Tolerances or ranges such as Maximum Penetration can be defined and limited for the user so that this value cannot be exceeded In the Tray Types Object details for the various Items are described Fur
81. ee it from the board 3 Install the replacement board in the reverse order After reconnecting the two ribbon cables to the board ensure that the cables are folded flat and can slide inside the X axis without damage Figure 53 Replacing Control X Board 85 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 Agitator Follow the sequence of steps shown in section F Description and Installation point 3 2 Assembling the COMBI PAL 5 Injection Unit Follow the sequence of steps shown in section F Description and Installation point 3 2 Assembling the COMBI PAL 6 Wash Station Follow the sequence of steps shown in section F Description and Installation point 3 2 Assembling the COMBI PAL 86 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation I Maintaining the PAL System Regularly performing maintenance helps ensure accuracy and precision of the PAL System Suggested intervals for maintenance procedures are given below to ensure uninterrupted operation If you use the system extensively for example nights and weekends or if you use corrosive solvents you may need to perform the maintenance procedure more frequently
82. eginning with PAL Firmware Version 4 1 X 79 PAL is a registered trademark of CTC Analytics AG Switzerland note syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation G Troubleshooting PAL and PAL x System The Combi PAL always represents one of several total components in a chromatography system The following Troubleshooting Guide is limited to the COMBI PAL or PAL xtonly Symptom or Error Message No or very low detector signal is observed Syringe does not fill properly Sample peaks responses are not reproducible Excessive carryover between samples Unexplained collisions of the Injection Unit Error Message Object Not Detected Error Message Invalid Motor Status Motor Aux1 Possible Cause Recommended Action Clogged syringe Remove syringe and aspirate dispense liquid manually Clean syringe Bent needle Inspect and or change syringe No sample liquid is injected Check and or adjust Needle Penetration into sample vial see section 4 2 Sample volume too low Air bubbles below Syringe plunger It can happen that air bubbles remain below the plunger after the first pull up If the plunger is moved up and down several times these air bubbles are worked out Dirty syringe Increase sample volume Increase method parameter Fill Strokes Increase Pst Cln Slv1 Slv2 values in method Use Pre
83. em after detection of a collision loss of steps of a stepper motor Stop XY Error 2 ON Automated recovery turned OFF Stop XY Error 2 OFF Automated recovery turned ON In PAL Firmware version 2 3 X the new feature that recovers automatically after detecting a collision has been implemented Firmware version 2 6 2 provides the possibility of disabling automated recovery If recovery is turned OFF the PAL system always checks its position before moving to the injection port for injection This can lead to an extra move crossing over the X Y axes sensors if the injection port is at the opposite end of the X axis relative to the sample location zero X axis position in between sample location and injection port For critical application fields e g clinical analyses it is advisable to turn off the automated recovery Stop XY Error ON PlgPathCheck The plunger travel path is monitored to ensure that the plunger tip reaches the syringe zero point in all cases compared to original plunger referencing and defining the syringe zero point If an error is detected the PAL System stops operation This feature is useful if sample solutions with particles need to be handled or if the syringe type is not adequate for the solution composition or if the syringe plunger is blocked or even bent such an error will be recognized and the system will come to an emergency stop PlgChnge Pos Changing a syringe using the Menu function Chnge Syr moves
84. es The accessories for the PAL System are described in the attached PAL accessories Catalog or on the web page www ctc ch 115 PAL is a registered trademark of CTC Analytics AG Switzerland
85. first injection Inj Time Diff 225 Drive needle to penetration depth 2 Inject to Injectors Needle Penetr Motor Z Motors Inj Penet Spd 24 Delay Pre Inj Del 25 Activate Injectd2 signal Injectd2 Out Signals Pulse Time PAL is a registered trademark of CTC Analytics AG Switzerland 98 system COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 26 Dispense syringe contents into Inject Speed second GC Injector 27 Delay Pst Inj Del 28 Retract needle from injector MotorZ Motors Inj Penet Spd 29 Clean syringe and Pst Cln Slv1 SYRINGE Syringes Fill Speed needle with solvent 1 Eject Speed Wash Wash Stations Needle Penetr Waste Injectors Needle Penetr 30 Clean syringe and Pst Cln Slv2 SYRINGE Syringes Fill Speed needle with solvent 2 Eject Speed Wash2 Wash Stations Needle Penetr Waste2 Injectors Needle Penetr 31 Move to Home position Home Positions 3 4 1 GC Dual Cycle Method Parameter Description Parameter CYCLE SYRINGE 1 Sample Vol 1 Air Vol 1 Inject to Inj Time Diff 2 Sample Offs 2 Sample Vol 2 Air Vol 2 Inject to Pre Cln Slv1 Pre Cln Slv2 Pre Cln Spl Int Cln Slv1 Int Cln Slv2 Pst Cln Slv1 Pst Cln Slv2 Fill Volume Fill Speed Fill Strokes Pullup Del Inject Speed Pre Inj Del Pst Inj Del Description GC Dual Selected syringe Sample volume for first injection Volume aspirated after the syringe needle is moved out
86. flush underneath plate of Tray Holder which corresponds to plate thickness of Tray Holder Measured in mm This item is available at User level in section Utilities Tray and can be used for fine tuning a Tray installed in a Tray Holder Important for well plates 584 installed in a Stack Remark Position 1 is the only correction point Path Offset X The Path Offset X can be specific for a Tray adjustment in X direction Expressed in mm Remark This item is only valid if a Path is assigned to the Tray Holder as specified in this combination Path Offset Y The Path Offset Y can be specific for a Tray adjustment in Y direction Expressed in mm Remark This item is only valid if a Path is assigned to the Tray Holder as specified in this combination Path Offset Z The Path Offset Z can be specific for a Tray adjustment in Z direction Expressed in mm Remark This item is only valid if a Path is assigned to the Tray Holder as specified in this combination dxRow Correction for inclination of a Tray in X Row direction For details see Section 4 1 2 2 above dYRow Correction for inclination of a Tray in Y Row direction For details see Section 4 1 2 2 above dzRow Correction for inclination of a Tray in Z Row direction For details see Section F Description and Installation point 4 1 2 2 above dxCol Correction for inclination of a Tray in X Column direction For details see Section F Description and Insta
87. ge Priming 10 If only one Job is to be processed select the Job with the scroll knob Press Start key In the dialog box Select Job s to Process select one of the following options All Entire Job Queue starting from the top e Selected Job selected with the cursor bars e Resume Continue with the next Job after the one aborted 3 2 Aborting a Job Queue 1 Press STOP 2 Select one of the available options Continue Sample Job or Job Queue 3 Select Continue to resume processing with the current sample 4 Select Sample if there is a problem with only the current sample Processing will resume with the next sample PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 5 Select Job to abort processing all samples in the current Job Processing will resume with the next Job The aborted Job is marked with an X 6 Select Job Queue to abort processing of all Jobs The JOB QUEUE screen will be displayed The aborted Job is marked with an X 3 3 Restarting an aborted Job Queue 1 Press the START key 2 Select the Resume option The job after the last one marked as aborted will be started 4 Utility Functions Utility functions selectable from the Menu screen provide quick access to checking operations and parameters that may need to be changed These functions are available fo
88. ginning with PAL Firmware version 4 1 X Please note that the service test Test Head is executed in the same way independent of the PAL Firmware version However the test evaluation is different between firmware versions 2 3 and version 4 The updated PAL Service Manual Edition 1 1 will include the PAL x System and will explain these differences 71 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 9 3 Section F3 Setup Objects For the section Objects opened in Extended User Mode the same applies as is described above New classes are visualized and known classes may contain new items accessible only on the level of the Extended User Mode To open this level use the following path Menu F3 Setup Objects Syringes Vials Tray Types Tray Holders Scroll down 2009 05 06 16 07 20 Home Riis wl Figure 47 Selecting Objects in Extended User Mode Object Description Syringes At this level all loaded syringes are selectable At the Utilities user level only the active syringe Is visible Vials The Firmware Objects accessed on the PAL Extended User Mode have more specific items selectable than at the User level Tolerances or ranges such as e g Maximum Penetration can be defined and limited for the user such that this value cannot be exce
89. gray plastic tube over the needle This plastic tube protects the syringe needle at shipment but it can be used as a syringe removal tool 6 Using the gray plastic tube push the Headspace Syringe through the heated Syringe Adapter Gastight Headspace syringes have very tight plungers when new After a short operating time at elevated temperature the plunger tip adjusts its diameter Should the syringe be used later at lower temperatures there will be a risk of leakage The polymer tip never expands at lower temperatures Therefore a particular Headspace syringe should always be used at the same or higher temperatures 6 Interfacing the COMBI PAL to Other Devices 6 1 Synchronization and Output Signals Synchronization Signals Sync Signals are inputs that tell the COMBI PAL when to wait or proceed with a sample processing step Output Signals Out Signals are sent from the PAL to external devices to indicate status or completion of particular processing steps These signals are classified as Objects Physical Events e g TTL In1 are also Objects and may be assigned to named signals PAL Cycles require that certain signals such as Start Inject and Injected be defined See the next table below for the PAL Events and signal assignments associated with the standard PAL GC Inj HS Inj and GC Dual cycles Certain types of integration of the PAL system into data handling or control software such as ChemStation Xcalibur
90. he Extended User Level provides various options to access other classes which are not visible at the User level Some classes are known but may show some new items Details are described below Menu F3 Setup Ir Ident Access Sounds Time Scroll down 2009 05 06 16 07 20 Home nininni Figure 46 Selecting Setup in Extended User Mode Object Description Ident The Identification of the system provides the opportunity to assign Users site and system names The serial number of the PAL System can be entered at this level Remark After loading a fresh PAL Firmware Backup file the SNo Is displayed as XXXXXX Access Access can be selectively opened or limited to different users for the Job Method or Setup Sounds The Beep sound can be adjusted to specific needs For safety reasons It Is advisable to keep the default settings especially the Warn Move turned ON to signal the start of the PAL System to the user Time The PAL internal clock can be set Remark If the dock is fast reset all items from Year to Seconds and use the mandatory function key F1 Set Time Communication The communication mode serial or LAN can be selected and conditions are defined For details see above point 8 or Addendum PAL Loader Software System Basic parameters can be predefined for specific needs Details see below Service Basic service tests can be activated Details see below Objects Access
91. her accessories 4 When placing the COMBI PAL onto a GC make sure that no objects interfere with either the Y axis or the Injection Unit throughout the entire range of potential movement 3 2 Assembling the COMBI PAL During headspace operation the syringe Is cleaned after each injection with clean inert gas e g Nz or He Therefore a 1 8 inch gas supply line must be available close to the COMBI PAL l Hirst mount the correct GC mounting kit onto the GC 2 Loosen the two mounting clamp Torx screws of the vertical legs 3 Place the X Y axes assembly on top and fit the mounting kit legs into the groove in the X axis Be sure that the clamps fit completely into the grooves Alternately tighten the two Torx screws until the legs are firmly in place 4 Double check whether the leg claws are correctly attached to the X axis see Fig 9 Wrong Correct Figure 9 Attachment of Mounting Claws 55 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 2 1 Installing the Injection Unit Installation of the Injection Unit should be done carefully When installing it for the first time have someone hold it in place while the mounting screws are inserted D Guide pins Figure 10 Attaching the PAL Injection Unit 1 Remove the three Torx mounting screws A B and C used to fix the Injection
92. ia uoneqrouj SJajatuPJed POULIN Tvd papuawworay auium Suiysnyy JAS pa lui Sq aq lur aud paads p luj 0 palu Pa dning paads iij dwaj adu S au JO By aul uo By paads By au jeqnou dwa jeqmul auinjoA ajduies JONIAS TDA ap poa Jaunpejnueul op Janpejnueui palu woy sap anjeA 10polu sun palu uonerauag 3 pa3N opelur JOY ur pajeau apu awn Jo uisus sajduies snoosis Aja soy pasn st Aep uomeluj aid sanuejod 1uaJagip Ajuramxa Jo sjuauoduio uim sajduies J0j pasn s z JUaAJOS Vea alg 98uu s sana SAM OS oc S3uuAs Tri O1 104 paads paly 0 0 0 soz 01S SGO g sw g sui 0 sw 0 swg sii 0z ois sim cot sfr oc LUD Liu 25 EJ SS 8S SS c c C 0 0 0 f mo l 01 60 MOLEO fot orco nol mor wot mol mo mol lur 55 lu 55 lur 55 Bursuoden ysnj4 pio palui Ald suuinio pa peg uomaluj uunj uo uomalu ds sjajuipIed poujay Tyd papuatuucoay ZNS UD Ed LAS u Ed Pa lul Sd pa fur aid Dead patuj 0 pafuj pa dning Sa0415 4 paads I4 3tunioN d d5 UD aug ZINS UD add LAS UD 2d aulnjoA Iy auinoA ajdujes 3ONIMAS mm pinbn apy poujaw PAL is a registered trademark of CTC Analytics AG Switzerland Figure 57 COMBI PAL Firmware Overview page 4 COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual system Installation and Operation 4 2 PAL COMBI X PAL System Flow Chart based on PAL Firmware Version 4 1 X Firmware version 4 1
93. ing the PAL Firmware dass Objects in the Extended User mode provides more detailed possibilities for object items At this level an Object can be copied or deleted by using the specific function keys This provides quick access to add e g a second or third injector Items displayed in italic are available beginning with PAL Firmware Version 4 1 X Items displayed in bold are explained in detail below 68 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 9 2 1 System The following System Items can be changed by selecting the particular item Beginning with PAL Firmware version 4 1 X an intermediate step was added After selecting System the new Settings level follows Activating Settings opens up the described Items below Item Description Syr Detect The syringe detection can be turned off in cases of sensor failure or if a spec syringe with unknown ID is installed Turning OFF the syringe identification sensor automatically displays a dialog box enabling the selection of syringe identification manually Start Ref This item provides the choice of referencing all axes at the start of the system or suppressing the referencing It is advisable to use this function actively to detect a possible loss of steps Stop XY Error This function allows turning off the automatic recovery of the PAL syst
94. ion specific Tray Holder A Tray Holder can hold one or more trays Each Tray Holder has a reference position X Y Z coordinates that defines its location 08 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Tray A Tray holds multiple samples Trays are defined by designating the Tray Type see below and the Tray Holder Tray names are used to identify the sample source within a PAL Job Tray Type A Tray Type defines the pattern and sampling sequence of sample locations within a Tray Stack A Stack is a particular type of Tray Holder that is designed to hold microplates A six drawer Stack holds 12 standard micro plates two in each drawer A three drawer Stack holds six deep well micro plates two in each drawer PAL Object Manager Software to load a PAL Object List to an instrument if a Module hardware module has been added to the PAL System In a special mode Object Manager can also be used to create and maintain Object Lists PAL Object List If a PAL Module hardware is added to an instrument several Objects have to be loaded into the firmware These Objects are collected in an Object List and stored in a file with the extension pol Object Lists are delivered together with Object Manager Software and are grouped into folders for the different kind of Modules e g Syringes Tray Holders
95. iquid Injections l Select Menu and press F1 Chang Syr The Injection Unit will move to a location that facilitates removal of the syringe Loosen the plunger retaining screw Move the plunger slightly out of the plunger holder Pull the syringe adapter out and then carefully upward to remove the syringe adapter with the syringe from the Injection Unit 5 5 Installing a Syringe for Headspace Injections l Select Menu and press F1 Chang Syr The Injection Unit will move to a location that facilitates installation of the syringe Fit the red plunger holder onto the plunger button Place the syringe in the appropriate heated syringe adapter Pull the black plunger out to approximately 20 of its length See Fig 39 Hex screwdriver Figure 39 Headspace Syringe and heated Syringe Adapter 4 f necessary loosen the plunger retaining screw in the red plunger holder 56 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Lift the lower needle guide sliding it into the upper needle guide Insert the syringe needle tip and match the magnetic pins of the syringe holder with the counter positions on the syringe slider Let the lower needle guide return slowly and make sure the needle tip does not catch on the guide See Fig 40 Press the heated cartridge firml
96. irrored to the Extended User Level The X offset is defined as the distance from the outer edge of the Tray in the X direction to the center of the vial position 1 Measured in mm The Y offset is defined as the distance from the outer edge of the Tray in Y direction to the center of the vial position 1 Measured in mm The Z offset can be selected for special cases where e g the syringe needle has to reach a deeper point before penetration into a vial A 7 offset value 0 considers the Tray surface or vial top as the reference point Measured in mm Items displayed in italic are available beginning with PAL Firmware Version 4 1 X The dimensions used for Row Column Length or X Y Z Offsets for Sample Number 1 are specific for a particular Tray Type If a general adjustment for a Tray from a specitic vendor e g for a Deepwell Plate has to be made this should be done in the Tray Type class of Object For fine tuning the Offset items from the class Trays can be used 76 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Explanation for various Patterns in Tray Type l Here Pattern Type Regular The sample or well positions are equal distributed Column 0009 N o age nl p up qp 12 3 4 5 6 7 B 38 101172 oog Oooog www D 3j I OO s OOU0000
97. jector Solvent not suitable for application GC Injector liner not suitable GC related parameters Dirty syringe Check pressure at flush gas regulator 0 5 bar during syringe flushing period Check gas flow at syringe needle tip Check recommended Method Parameters in Flow Chart Firmware Overview Appendix 4 The following parameters are crucial and should be verified first Hill Speed Pullup Delay Injection Speed Post Inj Delay See recommended actions above Check the parameters for injection technique If all changes give no positive result check the Injector Retract Speed See F3 setup Objects Motors Motor Z Check the boiling point of the solvent and try to change the solvent Check the liner geometry according to recommendations of the GC manufacturer Any parameter like injector oven or detector temperature can contribute to bad peak shape Check the column type and connections square cut connections Check the troubleshooting guide of the GC Manufacturer Increase method value Syr Flushing Set method value Syringe Temp 10 C above Incubat Temp None or too low Syringe Flush gas pressure Dirty flush gas HS syringe Cartridge holder temperature heater probe or sensor broken Agitator temperature heater probe or sensor broken Check pressure at flush gas regulator 0 5 bar during Syringe Flushing period Check gas flow at syringe needle tip
98. l the PAL Firmware Object Class Tool is also required See specific Addendum to PAL User Manual The release of PAL Firmware version 4 1 X makes possible the correction of all three axes X Y Z in the firmware class Tray The correction of the inclination can be made for a tilted Tray in the direction of the row and or the column Teaching is possible in the Utilities section by using the path Menu Utilities Tra ys Figure 23 illustrates possibilities of correction Details for teaching are given below Figure 23 Inclined Tray Corrections for X Y Z Axes Note that these functions are not available for the GOMBI PAL if operated solely on PAL Firmware version 2 4 X or higher but neither with firmware version 3 X X nor with version 4 X X 45 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 2 Description of Object Positions 4 2 1 Tray Holder e g THldr1 Figure 24 Tray Holder Reference Position For a Tray Holder the reference position is a hole in the base plate of the Holder The lower needle guide should be centered in the hole with the bottom of the needle guide flush with the bottom of the base plate Figure 24 shows the example of a Tray Holder for a single Tray e g THldr1 for Tray 1 with Tray Type VT98 4 2 2 Trays In general i
99. lation point 6 1 At this level no other functionality as shown at the User level Is provided The various synchronization signals are described above in Section F Description and Installation point 6 1 At this level no other functionality as shown at the user level is provided Three events are newly available on this extended user level Pwr Out1 Pwr Out2 and FlushVlv The main purpose of these three power out signals is to activate a solenoid such as for the Wash station or the Gas Flush Valve At this level the functionality of the signal can be tested Serial Valves or Multiposition Valve Drives are powered and controlled by the serial RS232 control and not through the AUX interface With firmware version 4 1 X a Mode is now selectable The mode refers to the setting of the Valve Drive whether the current for the motor is set to High Speed or High Torque For more details see Addendum to PAL User Manual Valve and Valve Drive The Out Expansion Box provides 8 TTL contacts and 8 relay contacts 24 V contact closure The optional module box is connected and controlled by Interface 2 For more details see Out Expansion Box Addendum to PAL User Manual This Object allows selecting the serial ports for the specific needs Choices are Remote Terminal Printer Barcode ExtDev VICI Vlv for serial valve drives or None 73 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI
100. ll syringe eject operations except sample injection Speed of plunger movement for sample injection Typically used for Fill Strokes Sets the temperature for a heated syringe during standby mode e g before or after a Job Job Queue is processed Indicates the actual current syringe temperature Plunger position during Chang Syr operation The syringe plunger is moved to a position where the syringe can be removed and replaced The value may be changed for different types of syringes Available for Agitator only 20 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 2 Tray After selecting the particular Tray to be accessed the following functions are available Function Key Description F1 Start Agi F2 Block NdlG F5 Movto nnn F4 HOME Item Needle Penetr Actual Temp F1 starts the spinning motion of the agitator It may be used to test the agitator function or to optimize the spinning speed for particular applications F2 activates Needle Guide blocking The option after activation is Rel NdlG releasing Needle Guide Blocking It may be used to test the functionality of the solenoid that blocks the needle guide This function serves as a quick check to determine if the X Y Z coordinates are defined correctly for the selected Tray To use this utility the select
101. llation point 4 1 2 2 above dyCol Correction for inclination of a Tray in Y Column direction For details see Section F Description and Installation point 4 1 2 2 above dzCol Correction for inclination of a Tray in Z Column direction For details see Section F Description and Installation point 4 1 2 2 above Items displayed in italic are available beginning with PAL Firmware Version 4 1 X 78 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation syste 9 3 3 Wash Stations The following object Wash Station items may be changed by selecting the particular item First select first the desired Wash Station such as Wash Item Description Position X X axis position for Wash Station Position Y Y axis position for Wash Station Position Z Z axis position for Wash Station Z Tolerance A tolerance window for a plus minus range expressed in mm where the Injection Unit has to expect an object If the value of this item is gt 0 this is a relative detection mode and the sensor from the Injection Unit needle guide Is active If the value of this item Is set to 0 the sensor of the needle guide is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object Z Retract The distance to lift up the syringe slider before the Y Z axes assembly moves acros
102. llation and Operation 3 2 4 Installing the Flush Gas Pressure Regulator Safety Warning Use an inert gas such as helium or nitrogen for the flush gas Hydrogen is highly flammable and explosive at certain concentrations 1 Attach the OUT side of the supplied pressure regulator at rear right hand side of the COMBI PAL See Fig 15 2 Connect a 1 8 inch clean gas supply line to the IN side of the pressure regulator Use a separate gas line to avoid any pressure drop on other equipment e g GC 3 Adjust the pressure of the gas supply source to 1 0 1 5 bar If a gas line Is teed up from an installed gas chromatograph the preset secondary pressure will be approx 5 bar 4 Adjust the COMBI PAL gas pressure regulator to approx 0 5 bar Pressure higher than 0 5 bar will not yield higher flow rates through the heated syringe The syringe needle is the point of flow restriction in the gas flow system The body of the pressure regulator can withstand up to 10 bar 5 Checkthe installed Flush Gas pressure regulator connections for leaks Avoid any contamination of the pneumatic system gas flow by using liquid soap or similar leak detection aids 6 Verify the Flush Gas pressure again during the flush period in a Headspace Cycle Figure 15 Installing the Flush Gas Regulator 39 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM
103. ly the following description of intended use therefore applying to the product groups PAL and PAL x which include for example the models Combi PAL HTS PAL or PAL Combi xtand PAL HTS xftogether with other PAL PAL xf design dependent subclasses If the PAL System is distributed through OEM partners the product or model name can be replaced with their own name but the Intended Use remains valid 6 2 Intended Use The PAL System is an XYZ robotic system offering high flexibility and functionality Its intended use can be described as being mainly as autosamplers or sample preparation devices for all relevant fields of application in chemical and physical analysis The systems will only be used by trained operators in a professional environment Typical fields of application for the PAL System are e Environmental analysis e Quality control in the chemical and pharmaceutical industries e Drug discovery e Metabolism studies on agrochemicals or pharmaceutical products The above list of typical applications is not exhaustive The traditional market position of CTC Analytics AG has resulted in the PAL System being mainly used as an autosampler device for the techniques of GC gas chromatography and HPLC high pressure liquid chromatography Sample preparation steps such as filtration purification or separation by means of solid phase extraction SPE derivatization etc are possible in combination with chromatographic
104. m Actual ID Fill Volume Fill Strokes Description Indicates the identification number ID of the currently inserted syringe If the syringe detection system Is set to manual the message Syringe No syringe is displayed This parameter serves to control the filling of the syringe It can occur that air bubbles remain below the plunger after the first pull up If the plunger is moved up and down several times see Fill Strokes these air bubbles are worked out With this operation the syringe can be completely filled even when using very small sample volumes Number of fill strokes All fill strokes except the last one use the selected fill volume If the selected sample volume is higher than the fill volume the sample volume is used for all fill strokes If zero is selected the plunger is pulled up only once using the sample volume value Pullup Del Fill Speed Eject Speed Inject Speed Standby Temp Actual Temp Plunger Chnge Pos By using this item a delay time can be selected between sample pullup and ejection while filling the syringe When the plunger reaches the zero position during the fill strokes the system waits half the Pullup Del time This allows for an air bubble to float away from the needle tip This feature is especially useful for removing any air bubble in the syringe and handling viscous fluids Speed of plunger movement used in all syringe filling operations Speed of plunger movement used in a
105. module on the PCB APR Control 7 65 COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 8 2 2 Special Remarks to the above listed Parameters DHCP If DHCP mode is active an automatic search for the IP address is activated If a free IP address is found the communication between the PAL xt System and the network becomes established The new IP address found through DHCP search is immediately entered into the PAL xt Communication protocol as seen from the local terminal The parameters IPAddr1 to 4 are not updated until one opens the communication menu again The Setup for the PAL Loader software requires entering the IP address or hostname The dynamically provided IP address is displayed on the local terminal As an alternative the following setup procedure can be used Prerequisite PAL Xf setting in Communication class DHCP On Open the Setup window and enter the following name in the Hostname or IP Address field CXXXXXX The Xs being the last six characters of the MAC address which is known from the parameter MacAddr4 MacAddr5 and MacAddre If a MAC address only consists of one character insert a O in front of it If for example MacAddr4 BA MacAddr5 35 and macAddr 3 enter CBA3503 and not CBA353 8 3 Troubleshooting for Serial or LAN Communication Both communication protocols are described in detail in the Addendum to PAL User Man
106. ms can be changed by selecting the particular item First select the desired Tray such as Tray Item Description Tray Holder The Tray Holder to which the Tray is assigned is specified TrayTypeGroup All Tray Type Groups which are allowed for this particular combination of Tray and Tray Holder are displayed The TrayTypeGroup for the Tray Type itself is specified for each Tray Type Tray Type The Tray Type to be used is linked to the Tray This item is usually set at User level in Utilities Trays See above Offset X The X offset is defined as the distance from the center of the teaching position from the Tray Holder to the edge of the Tray in X direction Measured in mm This item is available at User level in section Utilities Tray and can be used for fine tuning a Tray installed in a Tray Holder Important for well plates 584 installed in a Stack Remark Position 1 is the only correction point Offset Y The Y offset is defined as the distance from the center of the teaching position from the Tray Holder to the edge of the Tray in Y direction Measured in mm This item is available at User level in section Utilities Tray and can be used for fine tuning a Tray installed in a Tray Holder Important for well plates 584 installed in a Stack Remark Position 1 is the only correction point Offset Z The Z offset is defined as the distance from the plate surface of the Tray Holder to the teaching point black needle guide
107. n Keys 1 3 ESCape and STOP Keys 1 4 Scroll Knob and ENTER Button 2 Methods 2 1 Creating Methods 2 2 Edit View Methods 2 3 Delete Methods 3 Job and Job Queue 3 1 Building and Starting a Job Queue 3 2 Aborting a Job Queue 3 3 Restarting an aborted Job Queue 4 Utility Functions 4 1 Syringe 4 2 Tray 4 5 Injector 4 4 Wash Station 4 5 Vial 4 6 Dilutors 4 7 Tools 5 Logfile 6 Info Functions 6 1 Hardware 6 2 Software 6 3 Maintenance 6 4 Free Objects Free Items 7 Setup Functions 7 1 Sounds 7 2 Time 7 3 Objects PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation Table of Contents contd F COMBI PAL Description and Installation 28 1 General System Overview 28 1 1 Specifications 29 1 3 Electrical Specifications 30 1 4 Physical Specifications 5 1 5 Operating and Environmental Requirements 5 1 6 Sound Pressure Level 3 1 7 Hardware and Software Requirements 32 1 8 Regulatory Compliance Requirements 32 2 Product Warranty 33 2 1 Statement of Limited Product Warranty 33 3 Installation 35 3 1 Unpacking the Components 35 3 2 Assembling the COMBI PAL 35 3 3 Electrical Connections 42 4 COMBI PAL Object Positions 43 4 1 Defining Object Positions 43 4 2 Description of Object Positions 4
108. netr 20 Move to Home position Home Positions PAL is a registered trademark of CTC Analytics AG Switzerland 94 syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 2 1 GC InjS Cycle Parameter Description Parameter CYCLE SYRINGE Sample Volume Solvent Plug Slv Source IntStd Std Source Air Gap s AirVolumeNdl Pre Cln Slvi Pre Cln Slv2 Pre Cln Spl Fill Speed Pullup Del Inject to Inject Speed Pre Inj Del Pst Inj Del Pst Cln Slv1 Pst Cln Slv2 Description GC InJS Selected syringe Selected sample volume Volume aspirated to form sandwich with Solvent Plug Source Vial for Solvent Plug Volume aspirated to form sandwich with Internal Standard or Reagent Source Vial for Internal Standard or Reagent Air Gap s to separate Solvent Plug IntStd Reagent Sample Volume aspirated after syringe needle is moved out of the sample liquid Pre injection syringe wash strokes with Solvent 1 Pre injection syringe wash strokes with Solvent 2 Pre injection syringe wash strokes with Sample Speed of plunger movement used in all syringe filling operations Delay time between sample pullup and ejection Selected Injector Speed of plunger movement used during sample injection Delay time prior to sample injection Delay time after sample injection Post injection syringe wash strokes with Solvent 1 Post injection syringe wash strokes with Solvent 2 95 PAL is a regis
109. ng position 5 1 Building and Starting a Job Queue 1 Power up the COMBI PAL The JOB QUEUE screen is displayed JOB QUEUE OS X Stk 01 001 096 04 eml l 001 013 Method A Menu Add Del Start Job Job Figure 4 Example of Job Queue Screen PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation 2 Loada sample Tray onto an available location in a Tray Holder Note the corresponding Tray name If a sample Tray contains 10 mL sample vials and the HS Inj cycle is used insert the supplied adapters into the Agitator sample positions 5 Adda new Job for the Tray Press the Add Job key to bring up the default Job 4 For TRAY select the Tray name e g Tray1 that corresponds to the location of the Tray that was Just loaded 5 Enter the First and Last sample number for this Job 6 Select and enter the sample processing Method for this Job 7 Press the Home function key F4 to return to the JOB QUEUE screen 8 To add additional samples to be processed repeat steps 2 7 9 f necessary replace and or dean the syringe liquid versions only Press the Menu key to see the available options for changing F1 Change Syringe and cleaning F2 Clean Syringe To completely remove air bubbles the syringe should be primed manually See Section F Description and Installation point 5 2 Syrin
110. nsisting of 1 pc syringe heater 1 pc plunger holder 2 pcs syringe SYRC HS2 5 25 5 or 2 pcs syringe SYRC HS1 0 25 5 in combination with a Varian GC The supplied syringe kits cover the standard liquid and headspace GC injection techniques However certain applications may require different syringe sizes Consult COMBI PAL Accessories for detail 5 2 Syringe Priming for Liquid Syringe Types only It is critical that syringes be primed before beginning sample preparation Prime every liquid syringe first manually before inserting into the PAL system Afterwards the syringe can be primed and tested by using the Utility function Clean Syr 5 3 Installing a Syringe for Liquid Injections Before a syringe Is inserted the position Change Syr should first be verified The position Change Syringe should not be located above an object where the needle could collide with a vial as for example Path Menu Utilities Syringe tunction key F5 Change Pos Move the PAL Injection Unit to a location where a collision cannot occur by selecting the X and Y axes The value for the Z axis is given as a default and a change of this position is not necessary in standard operation 1 Select Menu and press F1 Chang Syr The Injection Unit will move to a location that will facilitate installation of the syringe 54 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA
111. on 2 2 1 or higher has been developed for the PAL xt System that in addition to serial communication also permits LAN communication TCP IP The software is backwards compatible and can be used for all PAL Firmware versions The PAL Object Lists have to match the corresponding PAL Firmware version as shown in the list below e PAL System Firmware Versions 2 X X PAL Object Lists Rev K or higher e PAL System Firmware Version 3 0 X PAL Object Lists Rev K or higher See specific Object Lists for this FW Version 3 0 X e PAL xtSystem Firmware Version 4 1 X or higher PAL xt Object Lists Rev B or higher The two Object Lists for the PAL and PAL x Systems can be loaded with the same PAL Object Manager software This simplifies the handling in cases where the PAL and PAL x Systems are in use within the same working environment Detailed information is available in the Addendum to PAL User Manual PAL Object Manager Software 61 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 8 Establishing Communication with Computer The PAL System allows a serial communication protocol with a PC As a new feature the PAL xt System provides LAN communication also referred to as TCP IP or Ethernet The PAL xt System requires a dedicated APR Control xf board and PAL Firmware version 4 1 X or higher
112. r deepwell plates without a plate seal Vapor phase from organic solvents can be hazardous and flammable Acidic vapor phase can cause corrosion to critical mechanical parts Disposal A Do not dispose of this equipment or parts thereof unsorted in municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment WEEE European Union customers Call your local customer service representative responsible for the PAL System for complimentary equipment pick up and recycling PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Commonly Used Symbols A Caution Risk of Needle Stick Puncture m oem 0000 Fuse mE Used with Main PAL Power Supply 0 Used with Main PAL Power Supply O o o Used with Optional Device s EON M Optional Device s Caution Risk of Electrical shock high voltage Disposal Do not dispose in municipal waste diii Follow local waste regulations to reduce electrical and electronic waste WEEE PAL is a registered trademark of CTC Analytics AG Switzerland system B Table of Contents A Safety Information B Table of Contents C List of Figures D How to Use this Manual E COMBI PAL Operating Instructions 1 Using the Control Terminal 1 1 Menu Screens 1 2 Functio
113. r injection Pst Inj Del 18 Retract needle from injector MotorZ Motors Inj Penet Spd 19 Transfer sample vial from Agitator to Agitator Tray Type Transport Mode Tray TRAY Tray Type Transport Mode 20 Move syringe to Flush position Flush Injectors Needle Penetr Move to Home position and skip remaining steps if Syr Flushing 0 21 Move plunger to open flush inlet SYRINGE Syringes Fill Speed and activate syringe flush valve 22 Delay Syr Flushing 25 Close syringe flush valve note Steps 15 and 16 can be activated before Step 14 Depending on the parameter setting Inj Signal selection PlgUp or PlgDown in class System Path Menu F3 Setup System PAL is a registered trademark of CTC Analytics AG Switzerland 96 syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 5 1 HS Inj Cycle Method Parameter Description Parameter CYCLE SYRINGE Sample Volume Incubat Temp Incubat Time Agi Speed Agi On Time Agi Off Time Syringe Temp Fill Speed Pullup Del Inject to Inject Speed Pre Inj Del Syr Flushing GC Runtime Description HS In Selected syringe Sample volume Temperature during incubation time Incubation time Agitator speed during incubation time On interval time during incubation time Off interval time during incubation time Syringe temperature Speed of plunger movement used in all syringe filling operations Delay time between
114. r or to perform an endurance test after a repair The Start and Stop positions of the test path can be selected The motor Current Speed and Acceleration are selectable accept default value if not secure A Pause time can be specified A Cycle Limit and Actual Cycle Counter are useful tools for endurance tests This tests the functionality of the PAL Injection Unit Head Two tests can be activated by the function keys FI Check Plg The stepper motor of the plunger drive is tested Sensor check F2 Check Z The vial detection sensor and Needle Guide Blocking function Is tested These tests are only required if any erratic behavior of the PAL System is observed In such cases contact a representative of CTC Analytics AG X Limit Actual status of X axis sensor Move the X Y Carriage manually across the limit switch to verify activation of the sensor Y Limit Actual status of X axis sensor Move the Y axis manually to the Y zero point at X axis crossing over the limit switch to verity activation of the sensor Z Limit Actual status of Z axis sensor Move the syringe slider assembly manually up to the zero Z axis point crossing over the limit switch to verify activation of the sensor Aux1 Limit Actual status of Aux limit sensor Aux2 Limit Actual status of Aux2 limit sensor Plg Pos Actual status of Plg Pos Needle Guide Actual status of needle guide sensor Items displayed in italic are available be
115. r the actual Syringe Trays Injectors and the Wash Station They allow access to key functions without having to set up and execute a Method and Job If an item is used in the sample processing cycle the appropriate Utility value will be overwritten by the Method value Menu Utilities Utilities Wash Station Scroll down 2009 05 06 16 07 20 EN ii Figure 5 Selecting Utilities Functions PAL is a registered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 4 1 Syringe The following functions are available by pressing a Function Key Function Key F1 Chang Syr F2 Clean Syr F3 Set Pos F4 HOME Description The syringe is moved to a position in which the syringe assembly can be completely lowered to facilitate removal of the syringe adapter The syringe can then be removed from the adapter and replaced A prompt will be displayed to specify the new syringe The syringe must be installed before pressing Enter See Section F Description and Installation point 5 Syringes This Function is used to clean or prime the syringe prior to use After selecting F2 either Wash1 or Wash can be selected Set Pos is used to define the Chang Syr position The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Syringe items may be changed by selecting the particular item Ite
116. r this Tray Type 715 PAL is a registered trademark of CTC Analytics AG Switzerland note syste Z Tolerance Z Retract Max Penetr Needle Penetr Spl1 Offset X Spl1 Offset Y Spl1 Offset Z COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation A tolerance window to give a plus minus range expressed in mm where the Injection Unit must expect an object If the value of this item is gt 0 this is a relative detection mode and the sensor from the Injection Unit needle guide is active If the value of this item is set to 0 the sensor of the needle guide is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object The distance to lift up the syringe slider before the Y Z axes assembly moves across the unit can be specified expressed in mm The maximum allowed Needle Penetration can be defined as a safety so that the user cannot destroy the needle tip by going too far down The Tray Type is prepared for a specific vial size this value is therefore unique to this combination The maximum needle penetration must not exceed the length of the syringe needle considering the loss due to needle guide etc Typical needle length is 51 mm maximum penetration must not exceed 48 mm This item is identical to that used on the User level or if available as a method parameter of a local cycle The value defined on these levels is m
117. r uwous se palqo AA apnpur JOU SBOP aJewjjos piepuejs au pood Do exueuejurey oju nuaN poods po Jojuno uoau pue axons JeSunjd paads j sin Jaq dnyng SONS Ill ges 3p 55 1 YUM suompeluj uap Z 994 gna SpA ajqeyeIas JeYIO SOg BU Sid duia jenpy aul JO Iv Suu i iy dwal Aqpuels paads pood an dwa jenpy Peeds Pel ZNS U duia Aqpueys leage E D E Go adi Aul Leni 1 aumnuny 55 pa lur isq ZNS UJ Isd n Ijguad 3 pa3N uini i Suiysn 4 JAS pq lul aug LAS U Id d dwa jenpy al Jenny pa ui Is paads pail pa il ISd dua Aqpueys aununy 55 pa uj aid o pal faq uy aug D uad a paaN Io moy rt mnooyeg oq pads palu padng peeds palu E 13003 aui qioseq o pelu paads it o palu BSA PN L teen ajouag lur padid ZNS up ald ja dnyjng 2 C ZM vO LXIS sog Bu Bid 0 qosaq paads I4 LAS UD Ald SAYONS ll4 CO 01 101 lS paads palu aui pen dwa a8uuAS pNauunjo ur paads iJ T SP eil ge Wave A aw go Bv deny aunor IY QD T sn Ii aul JO By dul UC IBy 90InOS DIS ids u ad paads pal NEN Z PSHO pa dning auii uo IBy paads i8y PiSiul TNS UD add Lene pyepueis elu 59 aie SOYONS ll4 paads i8y au jeqnou a2In0S NS LAS UID ad SUIN OA Itd dwa jeqrou dwa eqmu 8nd uaAo aunioA Jr aswH WD jeqnou Jeqrou ld JUSAJOS QA Jy 3uinjoA Iud OPM afa ad Aes QI jenpy SUI 2u Aig auinjoA ajdwes 4 auinjoA ajdwes 4 auinjoA ajdwes iL JAS JOIN AUAd apaaN Hauad APN lauadapaaN sAUAd APN JONIMAS
118. rameter Value Reference X Y Z axes and syringe System Start Ref ON plunger 2 Wait for Start signal Start Sync Signals 3 Read Barcode if enabled TRAY Tray Type Barcode None 4 Clean syringe with solvent from Pre Cln Slv1 SYRINGE Syringes Fill Speed Wash1 selected number of times Eject Speed Washi Wash Stations Needle Penetr Clean Volume Waste Injectors Needle Penetr 5 Clean syringe with solvent from Pre Cln Slv2 SYRINGE Syringes Fill Speed Wash2 selected number of times Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Waste2 Injectors Needle Penetr 6 Rinse syringe with sample selected Pre Cln Spl SYRINGE Syringes Eject Speed number of times Sample Volume TRAY Tray Type Needle Penetr Fill Volume Waste Injectors Needle Penetr Fill Speed Pullup Delay 7 Fill syringe with sample using Sample Volume SYRINGE Syringes Eject Speed several filling strokes aspirate air Air Volume Overfill outside of vial if Air Volume 0 Fill Volume TRAY Tray Type Needle Penetr Fill Speed Fill Strokes Pullup Del 8 Wait for Inject signal Inject Sync Signals 9 Move to Injector Inject to 10 Drive needle to penetration depth Inject to Injectors Needle Penetr MotorZ Motors Inj Penet Spd 11 Delay Pre Inj Del 12 Activate Injected signal Injected Out Signals Pulse Time 13 Activate Injectd signal Injectd Out Signal Pulse Time 14 Dispense syringe contents into GC Inject Speed Injector 15 Delay Pst Inj Del 16
119. rate air 1 Air Vol Overfill outside of vial if Air Volume 0 Fill Volume TRAY Tray Type Needle Penetr Fill Speed Fill Strokes Pullup Del 8 Wait for Inject signal Inject Sync Signals 9 Move to Injector 1 Inject to 10 Drive needle to penetration depth 1 Inject to Injectors Needle Penetr Motor Z Motors Inj Penet Spd 11 Delay Pre Inj Del 12 Activate Injected signal Injected Out Signals Pulse Time 13 Activate Injectd signal Injectd Out Signal Pulse Time 14 Dispense syringe contents into first Inject Speed GC Injector 15 Delay Pst Inj Del 16 Retract needle from injector Motor Z Motors Inj Penet Spd 17 Clean syringe with solvent from Int Cln Slv1 SYRINGE Syringes Fill Speed Wash1 selected number of times Eject Speed Wash Wash Stations Needle Penetr Clean Volume Waste Injectors Needle Penetr 18 Clean syringe with solvent from Int Cln Slv2 SYRINGE Syringes Fill Speed Wash2 selected number of times Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Waste2 Injectors Needle Penetr 19 Fill syringe with sample from job 2 Sample Offs SYRINGE Syringes Eject Speed sample number incremented by 2 Sample Vol Overfill 2 Sample Offs using several filling 2 Air Vol TRAY Tray Type Needle Penetr strokes aspirate air outside of vial if Fill Volume Air Volume 0 Fill Speed Fill Strokes Pullup Del 20 Wait for Inject2 signal Inject2 Sync Signals 21 Move to second GC Injector 2 Inject to 22 Wait for time since
120. re contact a representative of CTC Analytics 87 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation J Appendices 1 Definition of Terms Job Queue A Job Queue is a list of sample processing Jobs Jobs are executed in the order displayed on the JOB QUEUE menu screens New Jobs may be added to the queue while samples are being processed Job A Job contains the information needed by the PAL to process multiple samples by the same processing steps The elements of a Job are a Method and a Tray that define the location of the samples to be processed For identification Jobs are automatically numbered from 01 to 99 and then restarting with 01 when they are added to the Job Queue Cycle A Cycle consists of the specific operations necessary to process one sample The Cycle operations are repeated for each sample within a Job Cycles are designed for specific applications Method A Method defines how the samples are processed The elements of a Method are a Cycle a Syringe and a Parameter List Methods have names with up to eight characters and can be edited copied and deleted Method Parameters Method Parameters are associated with the Cycle operations User assigned Parameter values define how a processing operation Is performed A zero Parameter value will disable a Cycle operation Cycle Parameters are applicat
121. s exposed directly to liquids such as valves valve rotors or other valve components tubing or syringes or any other parts considered consumables and wear parts What Is the Period of Coverage This warranty remains in effect for a period of one year from the date of installation What will CTC Analytics do to correct Problems CTC Analytics AG is represented by an authorized distributing or OEM partner in the respective country These partners must be contacted to request technical assistance by phone to diagnose the nature and probable cause of any malfunction If we determine that the problem is due to the result of a defect in the instrument or a module any replacement module s that has have been determined to be necessary to correct the problem will be sent to user s location shipping costs at previously agreed conditions 55 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation What will CTC Analytics NOT Do A service representative will not be sent to perform service at customer s location prior to a determination by the responsible CTC Analytics representative that such is necessary Only after telephone support and replacement of any modules sent to user s site have failed to resolve the problem will we send a service representative on site CTC Analytics AG will not replace consumable parts as explained a
122. s shifted by 50 of hole pattern See Graphics below TrayTypeGroup Software protection so that not every Tray Type can be placed on any Tray Holder Example VT32 20 cannot be fit into a Stack Plate Thickn Thickness of Tray plate bottom not the total height of the Tray Measured in mm Vial Height Total height of the vial including cap to seal if applicable Measured in mm Remark The sum of the plate thickness and vial height adds up to the total height This is the point where the Injection Unit expects an object Vial Trnsprt The mode to transport a vial is defined by None No transport required Example liquid sampling Magnet Magnetic Transport Requires magnetic vial caps The Injection Unit moves to the side to wipe off the vial when moved away Needle Device is transported with the syringe needle inserted Example special vials where a magnetic cap cannot be crimped e g Vacutainer Remark This item became active beginning at PAL Firmware version 2 5 x ZSlideOffRetr If magnetic transport is selected the distance can be defined to move the syringe slider up Z direction lifting up the vial by magnetic force Measured in mm YSlideOffDist If magnetic transport is selected the distance can be defined to move the Injection Unit to the side Y direction to wipe off the vial Measured in mm Barcode If a Barcode Reader is installed the mode has to be set for AutoFix None No Barcode Reader option requested fo
123. s the unit can be specified expressed in mm Max Penetrat The maximum allowed Needle Penetration can be defined as a safety so that Needle Penetr the user cannot destroy the needle tip by going too far down The maximum needle penetration must not exceed the length of the syringe needle considering the loss due to needle guide etc Typical needle length is 51 mm maximum penetration must not exceed 48 mm This item is identical to that at the User level The value defined at this level is mirrored to the Extended User Level Type The specific mode for the Wash station in use has to be defined Standard Standard Wash Station Syringe is filled with wash solvent and expelled into Waste Pulse A pulsed signal is sent to activate the device Used for the Fast Wash Station and Active Wash Station See Type Active for details Flow Continuous flow of liquid by gravity No electrical contact required for a solenoid DLW Dynamic Load amp Wash Item used to activate specific solenoid for DLW Wash Station Active Used for Active Wash Station in conjunction with the Wash Station Print with PNo APR WASHPUMP B A Revision B Simplified print activation of pumps is controlled by PAL Firmware Requires Object Lists PAL WashActive Rev B Remark Type Active is not used for Fast Wash Station operation and cannot be used for first generation Active Wash Station with print Revision A installed For these ca
124. sample pullup and ejection Selected Injector Speed of plunger movement used during sample injection Delay time prior to sample injection Syringe flush time GC Runtime calculated from GC ready to GC ready 97 PAL is a registered trademark of CTC Analytics AG Switzerland system COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 4 COMBI PAL GC Dual Cycle No Processing Step Method Object Class Used Object Parameter Value Reference X Y Z axes and syringe System Start Ref ON plunger 2 Wait for Start signal Start Sync Signals 3 Read Barcode if enabled TRAY Tray Type Barcode None 4 Clean syringe with solvent from Pre Cln Slv1 SYRINGE Syringes Fill Speed Wash1 selected number of times Eject Speed Wash Wash Stations Needle Penetr Clean Volume Waste Injectors Needle Penetr 5 Clean syringe with solvent from Pre Cln Slv2 SYRINGE Syringes Fill Speed Wash2 selected number of times Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Waste2 Injectors Needle Penetr 6 Rinse syringe with sample selected Pre Cln Spl SYRINGE Syringes Eject Speed number of times Sample Volume TRAY Tray Type Needle Penetr Fill Volume Waste Injectors Needle Penetr Fill Speed Pullup Delay 7 Fill syringe with sample using 1 Sample Vol SYRINGE Syringes Eject Speed several filling strokes aspi
125. ses is the type Pulse required Flow Control The power source to activate a Wash Station is specified For the Fast Active Wash Station and DLW the Event Power Out1 and Power Out2 is used Ndl Cln Path A Path can be assigned to the Wash Station Waste to The Waste position is assigned to the Wash Station Clean Volume A percentage of the syringe maximum volume is defined for the cleaning step Clean Count The counter monitors the number of wash cycles The setting in the method has the same functionality and the value is mirrored to the Extended User Level Max Fill Spd The fill speed for the syringe is defined with this item If the maximum value of the Fill Speed 10 0 mL s is selected the system takes the injection syringe Fill Speed as defined in the method Max Eject Spd The Eject Speed is the speed used to eject the wash solvent during the wash cyde This speed is usually higher than the Fill Speed If the maximum value of the Eject Speed 10 0 mL s is selected the system takes the injection syringe Eject Speed as defined in the object dass Syringe Rinse Time Rinse is the time to rinse the wash port with wash solvent after the wash cyde of the syringe has been finished In the case of Fast Active Wash Station the syringe needle is pulled out of the wash port The wash solvent flows without restriction As a result higher solvent consumption has to be taken into consideration Items displayed in italic are available b
126. ss This item can be activated to avoid a vacuum if a relatively large amount of sample is taken out of a small volume vial If e g a volume of 50 uL is specified for injection the PAL system first takes 50 pL ambient air and injects this volume into the sealed vial causing overpressure in the vial After this step the sample volume can be aspirated without forming a vacuum This functionality is only valid for liquid sampling not for the Headspace technique Starting with PAL Firmware version 4 01X this item is also active in combination with Cyde Composer LC Inj Standard cycle for HPLC technique Details on method parameters see Appendices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview LC Cut Cyde for column switching with two valves HPLC technique Both valve drives have to be controlled through AUX interfaces Details on method parameters see Appendices point 4 Flow Chart Firmware Overview GC Inj Standard cyde for GC technique Details on method parameters see Appendices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview GC InS Standard cyde covering GC Sandwich technique Details on method parameters see Appendices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview GC Dual Cyde for GC technique which allows injecting two samples in two different injectors The start signal is sent after injection of both sample solutions Details on method parameters
127. t is not necessary to teach a Tray position The Tray Holder has a teaching position as described above A Tray is assigned to the Tray Holder The numbers values for the relevant items are stored in the firmware object Tray To complete the combination of a Tray Holder and a Tray it is necessary to assign the corresponding Tray Type to the Tray The Tray Type contains the geometric data for the Tray and information such as how many samples are in a row and a column in the Tray A Tray Type defines the pattern and sampling sequence of sample location within a Tray For details see below As described above the Tray is physically placed in the Tray Holder the firmware assigns the Tray to the Tray Holder and the Tray Type to the Tray This is routine procedure as long as no special circumstances need be considered such as customized Trays or Tray Types PAL Firmware version 4 1 X now makes a correction possible if the Tray surface is not exactly horizontal and planar but is inclined in one or more axes At version 4 1 X this is standard and a dialog window pops up the moment the Tray has been checked out at the corners to verify vial positions and heights How to correct a possible inclination is explained below 46 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation oon 1001 Jos zm i PE 77
128. tered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 3 COMBI PAL HS Inj Cycle No Processing Step Method Object Class Used Object Parameter Value 1 Wait for Start signal This is only Start Sync Signals checked at job start 2 Wait for Agitator Temperature Incubat Temp Agitator Trays 3 Wait for Syringe Temperature Syringe Temp SYRINGE Syringes 4 Transfer sample vial from Tray to TRAY Tray Type Transport Mode Agitator Agitator Tray Type Transport Mode 5 Move to flush position Flush Injectors Needle Penetr 6 Activate Agitator Agi Speed Agitator Trays Agi On Time Agi Off Time 7 Wait for Incubation timer Incubat Time Wait for Start Signal Start Sync Signal 9 Fill syringe with sample using Sample Volume SYRINGE Syringes Eject Speed several filling strokes Fill Speed Overfill Fill Strokes Agitator Trays Needle Penetr Pullup Del 10 Move to Injector Inject to Injectors 11 Wait for Inject signal Inject Sync Signals 12 Drive needle to penetration depth Inject to Injectors Needle Penetr MotorZ Motors Inj Penet Spd 13 Delay before injection Pre Inj Del 14 Dispense syringe contents into Inject Speed Injector 15 Activate Injected signal Injected Out Signals Pulse Time 16 Activate Injectd signal Injectd Out Signal Pulse Time 17 Delay afte
129. the exception of Maintenance are read only Menu Info Scroll down 2009 05 06 16 07 20 Figure 6 Selecting Info Functions 6 1 Hardware Item Description CPU SNo The serial number SNo of the PCB APR CPU is displayed CPU ID Version number of the PCB APR CPU MOTIO ID Version number of the PCB APR CPU 6 2 Software Item Description PAL Firmware Firmware version of PAL System Head Firmware Firmware version PAL Injection Unit Terminal FW Firmware version PAL Terminal Altera Firmware Firmware version of the Altera component 25 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 6 3 Maintenance Item Description PlgStrokeCnt This is a counter for syringe plunger movements The actual number of strokes is displayed If the counter reaches the set limit PlgStrkeLim a warning is displayed at the next start of a job run The system continues but signals the user to verify syringe conditions The counter can be set back to zero to restart There is only one counter for a syringe If syringe types are changed the system continues to count as if it were the same type PlgStrokeLim An upper limit for the syringe plunger strokes can be set Inject Count This counter monitors the number of injections The number of penetrations into the GC injector is a helpful tool for the user to decide on
130. thermore in this Firmware Object dass a Valve and or a Heater can be assigned Note that the Wash Station Positions Waste and Waste2 are listed in this Injectors dass 72 PAL is a registered trademark of CTC Analytics AG Switzerland syste Valves Agitators Dilutors Tools Positions Paths Sync Signals Out Signals Events Serial Valves Out Exp Box RS232 COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation The Valves and Valve Drives connected and controlled by an AUX interface are listed in this object class For more details see the Valves and Valve Drives Addendum Note that the valves controlled by serial communication Serial Valves or Multiposition Valves are in the PAL Firmware Object class Serial Valves At this level items such as Motor Drive Minimum Speed and Maximum Speed are selectable for the Agitator Object The range can be set for the speed The other items are available directly as Method parameters The Firmware Objects accessed in the PAL Extended User Mode have more specific items selectable than at the User level Tolerances or ranges such as Maximum Fill Speed can be defined and limited for the user so that this value cannot be exceeded Items such as Prime Volume Waste to Motor Drives or Scale Length are specific items that remain hidden at the User levels The Object class
131. time Is activated value gt 0 the solenoid of a Fast or Active Washstation opens for the specified time after the syringe needle has been removed from the wash port after completion of the syringe wash cyde The solvent flows into the wash port without the restriction of the needle be aware of higher solvent consumption The following Wash Station items may be changed by selecting the particular Item Item Description Needle Penetr By selecting the parameter Needle Penetr the Wash Station needle penetration value can be checked and or changed Rinse Time If rinse time Is activated value gt 0 the solenoid of a Fast or Active Washstation opens for the specified time after the syringe needle has been removed trom the wash port after completion of the syringe wash cycle The solvent flows into the wash port without the restriction of the needle be aware of higher solvent consumption 2 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 5 Vial After selecting the specific Vial type the following functions are available Function Key Description F3 Movto Vial The injection unit moves to the selected vial type By selecting the parameter Needle Penetr on the same screen the Wash Station Needle Penetration value can be checked or changed F4 HOME The Injection Unit moves to its HOME position
132. tor Speed of plunger movement used during sample injection Delay time prior to sample injection Delay time after sample injection Post injection syringe wash strokes with Solvent 1 Post injection syringe wash strokes with Solvent 2 95 PAL is a registered trademark of CTC Analytics AG Switzerland syste COMBI PAL LHX PAL PAL COMBI xt PAL LHX xt User Manual Installation and Operation 3 2 GC PAL GC InjS Cycle No Processing Step Method Object Class Used Object Parameter Value 1 Wait for Start signal Start Sync Signals 2 Reference X Y Z axes and System Start Ref ON syringe plunger 5 Read Barcode if enabled TRAY Tray Type Barcode None 4 Clean syringe with Pre Cln Slv1 SYRINGE Syringes Fill Speed solvent from Wash1 Eject Speed selected number of times Wash Wash Needle Penetr Stations Clean Volume Needle Penetr Waste Injectors 5 Clean syringe with Pre Cln Slv2 SYRINGE Syringes Fill Speed solvent from Wash2 Eject Speed selected number of times Wash2 Wash Needle Penetr Stations Clean Volume Needle Penetr Waste2 Injectors 6 Rinse syringe with sample Pre Cln Spl SYRINGE Syringes Eject Speed selected number of times Sample TRAY Tray Type Needle Penetr Volume Waste Injectors Needle Penetr Fill Volume Fill Speed Pullup Delay j Fill syringe with Solvent Solvent Plug SYRINGE Syringes Fill Speed Plug trom Slv Source
133. ual PAL Loader Software 66 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt n SYSTEM User Manual Installation and Operation 9 Special Functions This section describes special functions which can be activated by the PAL Extended User Mode The purpose of having two software access levels is to display discrete items and sections of the firmware at each level Settings which need to be done rarely perhaps at the time of installation of the PAL System are hidden at level 1 User Level and revealed at level 2 Extended User Level This also protects the PAL System in group use Nevertheless the important points should be known by the user and are described below 9 1 How to Access Extended User Mode Extended User Mode can be accessed by the following path Menu F3 Setup The above path as shown is an abbreviation for the following steps e Menu scroll the cursor to Setup e Press function key F3 e Activate Setup by pressing the center of the selector knob In this PAL User Manual or in technical notes only the following will be referred to Menu F3 Setup 67 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 9 2 Section F3 Setup The path to access this section is shown below This Setup section at t
134. xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 4 COMBI PAL Object Positions 4 1 Defining Object Positions Remove the Syringe Adapter from the Injection Unit before performing the following steps The objective is to define the Reference Positions for all COMBI PAL Objects Make sure the Tray Holder s Agitator and Wash Station are properly mounted to the PAL X axis The following description is an example of how to teach the reference position for a Tray Holder The described procedure Is common to all COMBI PAL Objects Figure 21 Object Reference Position For a Tray Holder the reference position is a hole slightly larger than the lower needle guide in the base plate of the holder The lower needle guide should be centered in the hole with the bottom of the needle guide flush with the bottom of the base plate see Fig 21 1 Switch ON the PAL power supply 2 Observe the keypad display The model name COMBI PAL will display along with the software version number The Job Queue menu screen will then display 45 PAL is a registered trademark of CTC Analytics AG Switzerland COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation 3 When the Job Queue menu displays complete the following sequence common to all objects Menu Setup Objects Tray Holders Named Tray Holder where Named Tray Holder represents a predefin
135. y against the Z axis slider to ensure that the Syringe heater unit engages Move the plunger up plunger holder until the thread of the screw catches the thread of the plunger bushing Tighten the screw to fix the plunger holder See Fig 40 The clamp provided with the Headspace syringe kit must only be installed with a 5 mL HS syringe See note enclosed in the syringe Kit V Figure 40 Installing and Removing a Headspace Syringe 7 Press Home The plunger moves down until it hits the mechanical stop This position is stored as the syringes zero volume position Then the Injection Unit returns to the HOME position 5 6 Removing a Syringe for Headspace Injections l Select Menu and press F1 Chang Syr The Injection Unit will move to a location that facilitates removal of the heated syringe adapter Unscrew the red plunger holder from the plunger bushing Move the black plunger slightly down to gain some space to be able to lift out the syringe cartridge Pull the syringe adapter out and then carefully upward to remove the syringe adapter with the syringe from the Injection Unit 57 PAL is a registered trademark of CTC Analytics AG Switzerland note COMBI PAL LHX PAL PAL COMBI xf PAL LHX xt PA SYSTEM User Manual Installation and Operation Figure 41 Removing a Headspace Syringe from the Heated Syringe Adapter 4 Unscrew the white plastic retainer 5 Place the supplied

PAL User Manual Installation and Operation COMBI PAL / PAL LHX

Contents

Download Pdf Manuals

Related Search

Related Contents