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Service Manual MCO-18AC MCO-18AC(UV)
Contents
1. Circuit diagram Control specifications Specifications of Sensor Prevention of contamination Operation check after replacing sensor and main Parts layout Test data Temperature pull up Humidity pull up Temperature change during power failure Temperature recovery 30sec 60sec door open Humidity recovery 30sec 60sec door open CO2 density recovery 30sec 60sec door open CO2 consumption and door opening Temperature uniformity 15points measured UV system kit MCO 18UVS3 Automatic CO cylinder changeover MCO 21GC __ How to reverse door How to install Roller Base MCO 18RB Instruction Manual Specifications aStrucutural specifications Heating system controller density display UV lamp
2. Wiring diagram UV LAMP 87 BL JL BL L BL ad D x O Y BL TOP HEATER 2 aol a FREQUENCY E 5 Sm E nus CHANGE SWITCH GLOW STARTER r BL WE OO 49 70 25 5 FG TP BACK HEATER tus o 8 12 45 ONLY AC100V BAMA HE w J E tel Lit BR Y BL m a os J Na SIDE HEATER R m m lt 4 d of 5 IE PS d oe 99H22 I BALLAST LP 88 t 5 6 VN pL m 8 el m 1 JL BL O wa Gua sou mx BK SIDE HEATER L Su Ou D a gt o 50Hz H E 5 X 2 MCO 18AC OPTION 47532 4X BL aw um t aa a eS ly NEN AS H i o gt 4 gt ge un CN14 CN301 e m 529 CN12 CNIO CN9 CN13 v LI E E A v r OVER HEAT a D se RLY2 RLY3 PROTECT RELAY m m e REMOTE ALARM UV LAMP RELAY ARBEL BOTTOM FRONT HEATER F m soi RELAY Vau Le 1 xmESUL 2 sm Le E D e sh x STE PWB EA lt a Gt 1 SAL BL Lu m gt mi Tuor MAIN HIMETAL 0008 O x RLY301 PM 8 t 5 a FAN MOTOR RELAY AT nd E TA UES 0 b PK AGITATING FAN MOTOR CNB 7 CNB CNA
3. 12 Clean all the attachments with a diluted neutral detergent and then rinse them thoroughly with distilled water 13 Wipe the trays the inner attachments such as the fan and the chamber sides with sterilizing alcohol Be careful not to leave any residual alcohol 14 Wipe the inside wall of the chamber with a gauze containing alcohol for sterilization and then wipe off with a dry gauze 77 28 29 15 MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed Wipe the water level sensor with sterilizing alcohol Be careful not to leave any residual alcohol When cleaning the sensor take care not to apply excessive force to the lead wires 16 To reinstall all the attachments perform the procedure in reverse order from step 11 17 Fill the humidifying pan with sterile distilled water Note When installing the fan insert it securely the motor shaft See Fig 10 Lightly turn the fan manually to make sure that it does not strike the rear panel Improper insertion may cause poor performance As shown in Fig 11 set the tray with only the front edge bent down If the tray is set in the wrong direction it may not be level and may become unstable Bent down 78 Filling the humidifying Use the following procedure to fill the humidifying pan or to replace the water 1 MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed Lift the humidifying pan cover Af
4. LEE WHITE FL BLUE IP ED YELLOW RED 2P AY TREER WH BLUE SR Connect after removing 5 Noize Mu z filter 1 Cover Water WHITE level T JP S EME 11 Attach the labels For Name plate use MCO 18AC UV label MCO 18AC 7 UV door switch Name plate caution label UV caution label 9 Patent label Fig 10 4727 12 Set the UV lamp period through control panel When using UV lamp option it is needed to set the UV lamp ON period Refer to the following procedure Description of operation Key Indication after operation operated 1 Turn the power switch ON The current chamber temperature is displayed 2 Press CAL key for 5 seconds CAL The left digit in the temperature indicator is flashed 3 By pressing ppkey M 4 key set the figure to F01 a 4 Press ENT key In CO density indicator x ENT 000 is displayed and the right digit is flashed 5 By pressing bbkey Fot 005 4 key set the figure to 005 5 minutes of ON period Press ENT key The setting of UV lamp ON time is ENT memorized and the current chamber temperature is displayed lt lt NOTE gt gt e The UV lamp ON time is settable between 0 to 30 minutes However it is recommended to set 5 minutes in general UV lamp ON time is settable between 0 to 30 minutes 000 to 030 The UV lamp is not turned ON if the setting is 000
5. gt Note F04 lt Operation gt lt Description gt lt Reset gt Setting of UV lamp 24hours sterilization mode Note It is necessary to select 001 installed in F20 beforehand Input F02 on temperature display and press ENT key 000 appears on CO display Input 001 with hn key and PP key to step to 24hours sterilization mode At the time press ENT key displaying with 001 to simply return to chamber temperature display When 24hours sterilization mode is set in F02 UV lamp starts illuminating and automatically goes off after 24hours has passed In the middle of 24hours sterilization if the door is opened UV lamp goes off and 24hours sterilization mode would be cancelled If 24hours sterilization mode is reset in the middle in F02 the mode would start operation from the beginning UV indicator should illuminate when UV lamp illuminates so the indicator will illuminate if UV lamp is uninstalled Input of service code 384 Note This function is only available for persons who know service code Input service code 384 to use the functions except for 01 02 F21 F22 F24 F25 and F30 Input FO3 on temperature display then press ENT key to show 000 on CO display 384 is appeared if service code has been already input Input service code 384 with key and Pp key and press ENT key to return to chamber temperature display Now the functions latter than are
6. when water sensor can detect water level for three times in 1 second interval lf water sensor cannot detect water level for five times it diagnoses that there is not water humidifying pan RH PAN lamp flashes when water sensor detects low water level in humidifying pan Timing 1 When a main power is turned on 2 When an outer door is closed 3 Every 30 minutes until an outer door is opened Note When an outer door is opened or UV lamp illuminates water sensor will be inoperative Example for LED displays Current temperature 36 2 C JE CO level 5 0 Set temperature 37 0 C 1 Value for CO zero calibration 5 3 Function F03 UV lamp accumulation time 135 HE I Service code input m gas error EO1 LC LE EJ I Bottom heater ratio 004 Lock Mode LO 25 Specifications of sensor Temperature Resistance for temperature sensor 103AT 1 Resistance DS 1 1300 35 694 _ 1256 em _ ro 87 es 6 3 62 _ n 39 60 _ 3078 40 589 1038 4 568 _ 963 43 526 928 44 5 _ 45 49 86e s am _ 831 4 as _ 802 _ 9 49 _ 746 50 1000 2 sm 26 Prevention of contamination In servicing for incubator it is important to prevent from contamination 1 More than 90 of contamination CO incubator is
7. gas cylinder empty alarm is emit and buzzer sounds after 2 7minutes elapse after CO inject lamp illuminates Ensure CO is injecting from the cylinder A to the cylinder Ensure CO inject lamp A starts blinking and the lamp is illuminating Ensure CO density should be reached to 5 and the unit should control properly Note The unit will break controlling during auto calibration in sensor The decimal point of CO display will blink during auto calibration CO2 sensor Stop injecting CO from the cylinder A and Eliminate the message E01 by pressing BUZZER key Ensure cylinder to be injected should be selected to the cylinder A Select the cylinder by pressing CO gas supply line switching key 43 How to reverse door 1 Removal of rear cover AS Inner door hinge Disconnect the power cable from the outlet and remove rear cover from the unit 2 Removal of inner outer door 1 Loosen and remove 5screws and remove frame panel lower from the unit 2 Loosen and remove 4screws then remove inner door hinge 3 Remove 2 lead wire connectors ground wire nylon clip and bushing then pull out the lead wire from the chamber Frame panel lower 4 Loosen and remove 4screws and remove 4hinge caps Rubber pads and brackets will be fallen off 5 Loosen and remove 8screws and remove the outer door Take care not to miss brackets lt Details of circle A gt Bushing
8. 86 Dutch Sanyo producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen de onderdelen kunnen worden gerecycled en weer worden gebruikt Het symbool betekent dat de elektrische en elektronische onderdelen wanneer deze vernietigd gaan worden dit separaat gebeurt van het normale huisafval Zorg ervoor dat het verwijderen van de apparatuur bij de lokaal erkende instanties gaat gebeuren In de Europese Unie wordt de gebruikte elektrische en elektronische apparatuur bij de daarvoor wettelijke instanties aangeboden Alstublieft help allen mee om het milieu te beschermen Swedish Din SANYO produkt ar designad och tillverkad av material och komponenter med h g kvalitet som kan atervinnas och ateranvandas Denna symbol betyder att elektriska och elektroniska produkter efter slutanvandande skall sorteras och lamnas separat fran Ditt hushallsavfall Vanligen lamna denna produkt hos Din lokala mottagningstation for avfall atervinningsstation Inom den Europeiska Unionen finns det separata atervinningssystem for begagnade elektriska och elektroniska produkter Vanligen hjalp oss att bevara miljon vi lever il 87 38 AUTOMATIC CYLINDER CHANGEOVER An automatic CO cylinder changeover system MCO 21GC is available as an optional accessory This system switches the gas supply line when one CO gas cylinder is empty Note The installation of the MCO 21GC must be performed only by qualified service
9. sensor box temperature is stable and high or low temperature alarm is not occurred it is judged as sensor malfunction if output voltage is lower than 1000mV or higher than 4800mV E11 and chamber temperature are displayed alternately and audible alarm sounds intermittently At the time CO valve is closed 16 12 13 14 16 18 Alarms Main heater is abnormal When main heater is open circuited or SSR for main heater is short circuited 12 and a chamber temperature are displayed alternately and buzzer sounds intermittently An error code is not always displayed just after failed because the self diagnosis for SSR and heater is done in every 40 minutes or when a power is supplied Bottom heater is abnormal When bottom heater is open circuited or SSR for bottom heater is short circuited E19 and a chamber temperature are displayed alternately and audible alarm sounds intermittently An error code is not always displayed just after failed because the self diagnosis for SSR and heater is done in every 40 minutes or when a power is supplied Door heater is abnormal When door heater is open circuited or SSR for door heater is short circuited E14 and a chamber temperature are displayed alternately and audible alarm sounds intermittently An error code is not always displayed just after failed because the self diagnosis for SSR and heater is done in every 40 minutes or when a powe
10. 2 digit in the temperature indicator Input FO3 by pressing pp key and 3 A ke A Key 000 5 displayed Input service code 384 by pressing p gt key and key zn m Press ENT key Current chamber temperature is displayed Press CAL key for 5 seconds ETS digit in the temperature indicator DIC F08 by pressing pp key and key is displayed on Press ENT key display and the right digit flashes Input 1 in the left digit by pressing on b gt key and 4 key NOTE You must not change the center and the right digits Current chamber temperature is displayed a and gas supply indicator A is lit or 1 will be displayed on marked GAS SUPPLY gas supply gas supply indicator A indicator B MCO 18AC 42 lt Operation check gt Ensure operation check should be done according to the following procedures after CO2 cylinder automatic changeover mode is setting DT Stop injecting CO from the cylinder and inject it from the cylinder only Turn the power switch on Set 37 for the temperature and 0 0 for density Ensure the cylinder A should be selected and the indicator A should be illuminated Leave the unit for approx 1 hour until density is stabilized Refer to the Instruction Manual for the detail descriptions about usage of MCO 21GC Set 5 for CO density and ensure the CO inject lamp illuminates Ensure E01
11. UV lamp is turned off when the outer door is opened while the lamp is on The lamp is turned for pre set duration after the outer door is closed condensation in the chamber be caused and or the chamber temperature distribution can be affected due to the heat from UV lamp when the setting of UV lamp ON time is longer than 5 minutes or the only the outer door is opened closed repeatedly such case the life of UV lamp is shortened e In the procedure 2 above pressing CAL key for 5 seconds causes the calibration mode this mode it is possible to calibrate the temperature and CO density and miss operation of the key may affect the basic performance of the unit Take care of the key operation Even if the key is operated wrongly the unit returns to the current value display mode automatically when no key is operated for 90 seconds n this case the setting not entered by pressing ENT key is not effective 13 Check that the UV lamp is turned on Check the UV lamp is turned on by the following procedure MEE poser Open the outer door and push the door switch with the inner door is closed Check that the UV indicator on the control panel is on after a few seconds 3 Check the visible blue light from the front side of the humidifying pan cover Check the light with the inner door closed Never look at the UV light directly 38 14 Set the connection of water level sensor option The setti
12. 3 4 screws M4 Cc 1 Model number label 17 1 humidifying pan cover 2 1 water level sensor assy Procedure 1 Unplug the unit and make sure that power is not 3 Take off the rear cover shown in Fig 2 supplied to the unit 2 Remove the duct from inside the unit then take off 2 caps and 2 screws shown in Fig 1 Rear cover Fig 1 Fig 2 1 4 Take out two insulations for the UV lamp Replace the duct Place the humidifying pan lead wire hole shown in Fig 3 then cover the pan with the humidifying pan The insulation can be taken out easily by cover as shown in Fig 6 pushing it from inside of the chamber Keep the insulation for the future use again Humidifying pan cover Insulation Fig 3 5 Set the UV lamp to the hole and make sure 8 Replace the insulation into the UV lamp lead wire to be fitted properly hole with the wire passed through the cut on the Fix the UV lamp cover with 2 screws insulation See Fig 7 Screw a UV lamp cover UV lamp lead wire Fig 4 6 Fix the packing and water level sensor assy with 2 screws See Fig 5 Insulation Fig 7 sap 9 Remove shown in Fig 8 By using 4 screws enclosed with this kit fix the ballast glow assy as shown in Fig 8 10 Connect each lead wire connector of ballast glow assy as shown in Fig 9 rc E oO g CNIS Q BLUE 2P Glow starter Main board CN14 L
13. Water level sensor CO inlet connection inlet pressure Accessories Temperature controller Temperature display Air filter Remote alarm contact Weight Optional accessories MCO 18AC UV 3 trays made of stainless steel containing copper HEPA filter Efficiency 99 97 or more Natural evaporation with humidifying pan Automatic set temperature alarm automatic set CO density alarm Upper limit temperature alarm Door alarm UV lamp failure alarm UV type only Low humidifying water alarm UV type only otacking plate A and B 2 protective stickers 2 tube bands UV system kit MCO 18UVS3 UV replasement kit MCO 20UV Gas pressure regulator MCO 100L Extra tray MCO 47ST including 2 tray supports Half tray MCO 25ST Automatic CO2 cylinder changeover system MCO 21GC Roller base MCO 18RB 4 20mA Interface MCO 420MA USA only RS232C RS485 Interface MTR 480 Ethernet LAN Interface MTR L03 SANYO Data acquision software MTR 5000 Standard aControl specifications MCO 18AC MCO 18AC UV Temperature display LED digital display Resolution 0 1 C CO controller Microcomputer Input by keypad CO density setting range 0 20 Resolution 0 1 Non volatile memory density display LED digital display Resolution 0 1 Water level detection Option Water sensor Optic type Key lock function Settable by pressing digit shift key for 5seconds Self diagnosis functi
14. amp E e El x mo c l 10 RED PK PINK 8 D pete E EN O ORANGE 8 Y YELLOW 8 oe z 2 101 BL BLUE L BL LIGHT BLUE 2 5 s GN GREEN amp WHITE B 20 EUR n B E Xii 31 0 GY GRAY R BR BROWN wet MTR 480 iwi BK BLACK 8 S SHIELD 9 8 vor of E CO 18AC m ASN id OPTION DISPLAY O 4 o e LI t _ 5 040 oc CIRCUIT DIAGRAM MCO 1 MCO 1 UV 12 sans cetim nam nam 3 g i i ELSA dsaH N gt Pp 5 1 4 oF o 1 I OE lt zt i 5 N EW bes IN 1 3 1 4 2985 09817 1 p i bad m ALIOLANH ls 408 lt 9 09 rs i t t a O D 1 1 er o oi x Ea COVED OLBS 1 I 0 0 0185 3 mE 0 0 0185 POIG H 1 TOVED OLES 010 1 z EDVED OIES 2010 i d 0v 0 OIBS 1010 JDE AE HBE E a AZ 468045 H 1 NIVA U3LVIH WAH i i H u3lv3H i av ts E i a 5 a i 1 AVI34 LV3H DA XLEPOPLIAA E
15. Ballast 0 109 27 822 5 Ana J109 e go EN GENS 82 CN302 E qnoi AT 1 heater J144 Back heater Side heater R Side heater L Flange heater 3 1 8 8 13 swio 7 2164 swoz 12 54103 24 LE C102 0102 8 LE BUZZER eu LT ps 43 Bottom side heater R HAS CNS Bottom side heater L Power transformer Bottom front heater CN2 Bottom heater 1 2 5 Front panel heater Switching power supply CN2 Bimetal thermostat Door heater C5 Hinge sub heater R Hinge sub heater L CNI2 4 O O ro CN13 C55 Buzzer TH eas oo 8 1 XI R24 E C32 E R20 021 Ra 1 10 al on Remote alarm relay 1 COM ES CN303 ie E 4 3 Agitating fan mot 3 2 1 gitating fan motor 1 CNI 6 gt AB 037 5 8 2 MTR 480 CN4 1 2 Overheat protect sensor C 3 4 Temp sensor Water sensor CN8 Display PCB 1 2 Door switch Connection on PCB Connections on Main PCB Power transformer 1 2 AC8 5V 3 4 5 AC18V Switching power supply 1 2 GND DC5V To supply the power to PCB To supply the power to PCB 1 2 CO sensor Input from CO sensor Pe FEM valve Option commente ton neo Onin CN12 3 4 Hinge sub heater R L D measure the temperature of
16. E re 72 U gt wn 2 gt x 10 to gt gt e L lt a gt 0 0188 o ER Ses Bi thes E a BEN T bar D i ien ll AE gt RL EL EL 3 1 m oid i i EE oss ET ponn Do ESTOS LE C Eoo sea c Q I gt 25055 Do 9089 5225 55555 a ee 99 lt lt n o i a 144 a E 0X D CN Fi O e 27 aa 3 5 72 190 o SE x a N a eo z e e 5 o DO NOT SCALE THIS DRAWING s Cos Lid Unused area marked with SANYO Electric Bionedical Control specifications _ 1 Keys on Control panel BUZZER When an alarm LED is flashing and the buzzer sounds Buzzer and remote alarm output keeps activating SET Press this key to step to temperature setting mode Press this key two times to step to CO level setting mode Press this key three times to step to check mode of overheat protect temperature Press this key four times to return to chamber temperature display P gt A digit will shift among 2 digit 15 digit and 1 decimal digit by pressing this Digit shift key key Numerical value shift key CAL ENT A B When chamber temperature is displayed press this key fo
17. Equal or less than 80 R H Usable environment condition Designed performance may not be obtained when ambient temp is equal or lower than 15 C Note Specifications will be subject to change without notice WAN IN S N R R N N N S S N S N N N N Power cable port Access port Back side gas circuit injection port X CO2 sampling should be done through the port on the inner door Temperature calibration Note Ensure to cancel Key Lock prior to commence calibration 1 Press CAL key for approx 5 seconds 2 The 3 digit on temperature display flashes and other digits will go off 3 Input current chamber temperature by pressing Pb key and 4 Press ENT key to return the device to chamber temperature display Example When a setting temperature is 37 0 C and an actual measured temperature is 36 8 C Press CAL key for approx 5 seconds 3 digit on temperature display blinks and other digits will go off Input 36 8 C by pressing bb key and key ONO Press ENT key to return a device to chamber temperature display Note temperature calibration it is important to measure chamber temperature with accuracy Ensure to measure center temperature of the chamber Input range is within 1 0 C per calibration If a value which is out of the range is input an error tone w
18. Ground wire Hinge cap AD Nylon clip 44 3 Outer door preparation 1 Remove the cover from lower part of the outer door and pull out the lead wire to the opposite side Install the cover back to the position 2 Install the hinges and caps the opposite side such as the picture below Torque More than 80kgf cm Screw Screw 4 Inner door preparation 1 Remove E ring from inner door hinge pin then install inner door hinge in the opposite side such as the picture below Fit the E ring to the inner door hinge pin Inner door hinge pin Inner door hinge pin 4 EN E ring 5 Unit preparation 6 Install the caps Middle caps Small and caps Large inner door latch bracket and screws in the opposite place such as the picture below Inner door latch bracket Cap Middle Cap Small Screw Cap Large Installation of inner outer door 1 Install the outer door to the unit and connect the lead wire connectors and ground wire Hold wires by nylon clip Take care not to become lead wires taut when the outer door is closed Torque More than 80kgf cm 2 Install the bushing in the place where lead wires pull out from the chamber Install the frame panel back to the position 3 Install the inner door by tightening 4screws 4 Remove the caution sticker that is upside down Put the new caution sticker on the specified place p Gr und wire
19. al final de su ciclo de vida no se debe desechar con el resto de residuos dom sticos Por favor deposite su viejo televisor en el punto de recogida de residuos o contacte con su administraci n local En la Uni n Europea existen sistemas de recogida espec ficos para residuos de aparatos el ctricos y electr nicos Por favor ay denos a conservar el medio ambiente 85 36 37 Portuguese O seu produto SANYO foi concebido e produzido com materiais e componentes de alta qualidade que podem ser reciclados e reutilizados Este s mbolo significa que o equipamento el ctrico e electr nico no final da sua vida til dever ser descartado separadamente do seu lixo dom stico Por favor entregue este equipamento no seu ponto local de recolha reciclagem Na Uni o Europeia existem sistemas de recolha separados para produtos el ctricos e electr nicos usados Por favor ajude nos a conservar o ambiente em que vivemos Italian II vostro prodotto SANYO stato costruito da materiali e componenti di alta qualit che sono riutilizzabili riciclabili Prodotti elettrici ed elettronici portando questo simbolo alla fine dell uso devono essere smaltiti separatamente dai rifiuti casalinghi Vi preghiamo di smaltire questo apparecchio al deposito comunale Nell Unione Europea esistono sistemi di raccolta differenziata per prodotti elettrici ed elettronici Aiutateci a conservare l ambiente in cui viviamo
20. oO O Door open for 60sec Temperature C 34 33 32 0 10 20 30 40 Time scale Minute Humidity recovery 100 Door open for 60sec 95 Door open for 30sec Humidity 0 10 20 30 Time scale Minute 40 CO2 density recovery 6 Door open for 60sec 5 o S 4 Door open for 30sec 2 2 3 TD N 2 O O 1 0 0 10 15 20 25 30 35 40 Time scale Minute CO2 consumption and door opening gt 1200 Leak 4 8 30min 1000 800 S 600 400 S 200 o gt gt 1 2 3 4 5 Frequency of door opening day lt Temperature uniformity 15points measured gt Note Following data are the reference only Temperature at each measured points eiiescured polis 1 3692 Back A O 107 gt 6 Front 6 370 _ AT 3709 7 9 370 220 220mm 105mm Test conditions Ambient temperature Setting temperature Setting CO2 density Center of shelf 50mm away from each corner Overlook view UV system kit MCO 18UVS3 11 Kit of MCO 18UVS3 v 5 1 UV label 1 UV caution label CD 1 UV lamp 7 1 UV door switch caution label 5 1 Name plate 4 1 ballast amp glow assy 2 1 UV lamp cover SEE Er Q 1 Patent label
21. or step on the power supply cord or never damage or break the power supply plug A broken supply cord or plug may cause fire or electric shock D Do not use the supply cord if its plug is loose Such supply cord may cause fire or electric shock Never disassemble repair or modify the unit yourself Any such work carried out by an unauthorized person may result in fire or electric shock or injury due to a malfunction Disconnect the power supply plug if there is something wrong with the unit Continued abnormal operation may cause electric shock or fire O When removing the plug from the power supply outlet grip the power supply plug not the cord Pulling the cord may result in electric shock or fire by short circuit Disconnect the power supply plug before moving the unit Take care not to damage the power cord A damaged cord may cause electric shock or fire Disconnect the power plug when the unit is not used for long periods Keeping the connection may cause electric shock current leakage or fire due to the deterioration of insulation If the unit is to be stored unused an unsupervised area for an extended period ensure that children do not have access and that doors cannot be closed completely The disposal of the unit should be accomplished by appropriate personnel Remove doors to prevent accidents such as suffocation D Do not put the packing plastic bag within reach of children as suffocation
22. setting 37 C 5 no load Heat emission Temperature 5 C to 35 C Humidity equal or less than 80 R H tes The designed performance be obtained when pd the ambient temperature is equal or less than 15 C Note Theunit with CE mark complies with EC directives It is based on our measuring method 43 92 AN CAUTION Please fill in this form before servicing Hand over this form to the service engineer to keep for his and your safety Safety check sheet 1 Incubator contents Yes LINo Risk of infection Yes INO Risk of toxicity LlYes LINo Risk from radioactive sources Yes No List all potentially hazardous materials that have been stored in this unit Notes 2 Contamination of the unit Unit interior Yes LINo No contamination Yes LINo Decontaminated Yes LINo Contaminated LlYes LINo Others 3 Instructions for safe repair maintenance of the unit a The unit is safe to work on Yes No b There is some danger see below lYes No Procedure to be adhered to in order to reduce safety risk indicated in b below Date Signature Address Division Telephone Product name Model Serial number Date of Installation incubator MCO 18AC MCO 18AC UV Please decontaminate the unit yourself before calling the service engineer 93 44 SANYO SANYO Electric Co Ltd Printed in Japan
23. 119V 89W 36V 0 9W SVDC3A 85 265VAC 5 3 53 103AT 1 ___ 1 10K Q 25 C 220 240V EAE eine EE 4415Q 17630 2 d 50140 27790 E 243200 DC24V DC24V 250VAC 10A 250VAC 10A 28V 50mA _SVDC DC5V LLELZOTTYSMP J LATR HNZSST 230V 8W 230V 120 230V 30W J 230v 3TW 230V TOW 230V 10 4W LVAN 230V 3 230VBSW 36V 0 9W _FAB11 X2265 J _FABTT X2265 2UG2210118 BAM210131 5516 45 OLS 1003S ll AC220 240v 50Hz AC220v 60Hz 5 3 53 10 1 ___ 1 10K Q 25 220 240V 230V ETE 4415Q 1763Q mE 230V TOW _ oO 230V 10 4W 50140 27790 ER 4320Q S S ON UC ORE EABTTI X2269 LL DC24V DC24V 250VAC 10A 250VAC 10A 28V 50mA ZWSTS SH _5VDC 3A 85 265VAC DC5V FLZO0TTYSMP LATR HN2SST 230V BW _ 230V 12W 230V BOW LL 230V 3TW 230V 14W _ 230V STW __ _230 89W 0 36V0 9W 0 FABTI X2265 _2062210 115__ _ 210131_ SS AO a Walersensor 2 UV lamp 2 b 2 A S T S T Glow starter FG 7P FG 7P FG 7P Ballast 2 1 EKF OMASBID EKD 0423A22B EKD 0423A22B _ Rating AW 220 230V AW 220 230V 9u4 _ AW 115V Components included in option kit 1 CO2 auto changer kit MCO 21GC 2 UV lamp kit MCO 18UVS3 11
24. 37 0 5 the figure of settable digit changes Set temperature is memorized Press enter key ENT Left digit in the digital CO density indicator blinks By pressing digit shift key and numerical value shift key set the When pressed figure to 05 0 the figure of settable digit changes e Press enter key Set CO density is memorized In the digital density indicator HI is displayed condition In the digital temperature indicator upper limit Adjust upper limit regulator so that temp is displayed the alarm temp is 1 C higher than The upper limit temp can be changed set temperature by turning upper limit regulator This is the end of set mode and the indicators Press enter key ENT display current temperature and density e In each set mode if the change of the setting is not necessary pressing set key SET skips to next set mode e When the density is set to 00 0 the control is OFF regardless of chamber density e The upper limit temperature set value will change when the regulator is turned even if the incubator is not in set mode because the alarm circuit is an independent circuit In each set mode the indicator returns to the current temperature and density display mode automatically when 90 seconds has passed without any key operation 67 18 UV LAM P coi 8AC UV or
25. Auto Zero forcibly performed within a minute Press ENT key to come to the end because Auto Return is ineffective in this mode CO is not injected during 5 is displayed a DO FOG lt Operation gt 7 lt gt FO8 lt Description gt lt Operation gt F10 lt Operation gt lt Description gt F11 lt Operation gt lt Description gt Setting of output ratio on bottom heater Factory default 7 Range 0 49 Input F06 on temperature display and press ENT key to show a current setting value on CO display If you want humidity higher than current setting input bigger numerical value If you want humidity lower than current setting input smaller numerical value Press ENT key to store the value and automatically returns to chamber temperature display Setting of output ratio on door heater Factory default 5 Range 0 19 This function is for the prevention of dew condensation on glass door Input F07 on temperature display and press ENT key to show a current setting value on CO display If there is dew condensation on a door input bigger numerical value on CO display Press ENT key to store the value and a device automatically returns to chamber temperature display Setting of Auto Changer mode and Demonstration mode Factory default 000 Auto Changer mode One of lamp either A or B which is in current used illuminates By pressing A B key a cylinder which is in
26. a N Lead wire connector SK Bushing Nylon clip Details of circle B 46 Il How to install Roller Base MCO 18RB 1 Align 4 pcs of leveling plate on the Roller Base D to match them to each holes 2 Fit the fixing metal to each leveling plate and secure them by 2 screws Leveling plate Instruction manual This section is extracted and printed from Instruction Manual If you find out Refer to page 00 in them this page means not page in Service manual but page in the lower corner of each page in the extract from Instruction Manual This page number is not corresponded with serial number in Service manual attached Instruction Manual corresponds to the specifications for initial production so the contents may be changed without notice 48 SANYO MCO 18AC UV MCO 18AC INSTRUCTION MANUAL Incubator CONTENTS INTRODUCTION PRECAUTIONS FOR SAFE OPERATION LABELS ON THE INCUBATORS ENVIRONMENTAL CONDITIONS INCUBATOR COMPONENTS Control panel and keypad Remote alarm terminal INSTALLATION SITE INSTALLATION Connection of gas cylinder PREVENTING CONTAMINATION PRECAUTIONS FOR CULTURES START UP OF INCUBATOR SETTING THE CHAMBER TEMPERATURE AND CO DENSITY UV LAMP MCO 18AC UV or MCO 18AC with MCO 18UVS3 Precautions when using the UV lamp Setting the UV lamp ON period Lighting the UV lamp for 24 hours KEY LOCK FUNCTION WATER L
27. become disconnected Ensure that the gas pressure is set at the specified value Improper connection of the gas pipe or use of incorrect gas pressure may result in leakage of gas Elevated level of gas be hazardous to health and may lead to asphyxiation and risk of death 1 Use a liquefied CO gas cylinder not a siphon dip tube type CO gas should be 99 5 or more pure 2 Install a gas pressure regulator optional accessory MCO 100L on the cylinder Use a regulator rated at 25 MPaG 250 kgf cm G 3600 psiG on the primary side and 0 2 MPaG 2 0 kgf cm G 30 psiG on the secondary side 3 Using the gas supply pipe provided connect the gas pressure regulator to the connecting port for gas pipe located at the rear left side of the incubator Note If CO is supplied to multiple incubators from a single gas cylinder CO solid will be formed in the gas pressure regulator The gas pressure regulator safety valve will operate and there may an explosive sound When the MCO 21GC is not mounted Using the gas supply pipe that is provided connect the gas pressure regulator for the gas cylinder to gas pipe connection port A on the incubator After connecting the pipe check to make sure that no gas is leaking When the MCO 21GC is mounted Connect a pair of gas cylinders with CO gas pressure regulators independently gas supply line will be switched a
28. caused by mold Mold is a kind of true fungi it has high increasing power under high temperature and high humidity Accordingly it is easy for mold to increase inside incubator Those increased mold eat up culture mainly animal cell eventually precious test sample will be killed Cell that is independent from ecosystem is also independent from immune system it has no immunity against all 2 Precautions for mold increasing There are many cases that mold multiply by eating fat on the fingers as a source of nutrient in CO incubator Therefore Be sure to wash and sterilize your hands prior to servicing Use 70 ethanol installing sterilize the inside wall and shelves after removing shelves duct humidifying pan and so on with 7096 ethanol 3 In the case that mold has already multiplied Sterilize the interior walls and shelves with 70 ethanol after removed all items from the chamber as same as 2 2 above And you should sterilize again after 24 hours once sterilized when the root of spore is geminated because it is too difficult to remove spore of mold Repeat sterilization is effective NOTICE In general stainless steel is known that itis not easy hard to rust However depends on the conditions rust might generate When you remove rust we recommend the following agents Besides the surface may become foggy Recommended agent 1 Cream cleanser Gif Nippon Leva 2 Picasso SUS300 W Chemic
29. current used stops provision and a unused cylinder starts provision If a cylinder becomes empty its lamp starts flashing and another cylinder starts provision automatically Demonstration mode This function is used in a demonstration device pretends to control temperature but heaters are not powered and CO valve is not opened Auto Changer mode Input F08 on temperature display and press ENT key to show a current setting value in the 3 digit of CO display 0 Normal mode 1 Auto changer mode Demonstration mode Input F08 on temperature display and press ENT key to show a current setting value in the 2 digit of CO display 0 Normal mode 1 Demonstration mode Display of adjustment value for zero calibration Factory default 000 Input F10 on temperature display and press ENT key to display an current adjustment value for CO zero calibration on CO display If you press ENT key or there are no key operations for 90seconds a device automatically returns to chamber temperature display When a current adjustment value for zero calibration is 1 3 and zero calibration is performed 1 3 is the adjustment value for zero calibration At the time the display in F10 is 13 Display of an adjustment value for span calibration Default setting 100 Input F11 on temperature display and press ENT key to display an current adjustment value for span calibration on
30. each parts 3 4 Temperature sensor parts CN1 CN2 CN4 CNS CN6 N8 Connections on power PCB CN301 Main PCB 1 Heater for heat control 5pcs 3 Bottom heater 4pcs To control heater 5 Front panel heater Door heater 2pcs CN303 3 Circulating fan motor To control circulating fan motor 10 Electric parts CO sensor Type Code io AMEN 4 Overheat protect sensor Circulating Fan motor Power transformer DC 5V power supply Secondary Resistance Resistance Resistance Secondary Type Primary Rating Rating Rating AC110 120V 60Hz HS 3C S3 MIR 1000C02 103AT 1 L 599AT 1 10K 25 110 115VAC 115V Resistance Back heater Rating Resistance Side heater Rating Resistance Flange heater Rating Bottom front heater Rating Bottom back heater Rating _ Bottom side heater Rating Front panel heater 5 LH NI Hinge sub heater M Bimetal thermostat 2 valve 2 auto changer valve 1 Noise filter pus 4 Type Primary do 16479 11040 4410 4320 11499 13080 9450 3537Q 156 9Q 4320Q FAB11 X2265 DC24V 11 2265 DC24V 2UG2210931 250VAC 10A 7 250VAC 10A 5516 45 28V 50 2 515 0 DC5V ATRANSSHUT NOI 119V 10 4W MSM S
31. eyes Never turn on the UV light with the inner door or humidifying pan cover opened e The recommended replacement time for the UV lamp i e when the UV output ratio drops to approx 70 of its initial value is when the accumulated ON time reaches 1 000 hours When the accumulated ON time reaches approximately 1 000 hours the UV indicator blinks when the UV lamp is not lit It is recommended that the UV lamp be quickly replaced at this point Contact a Sanyo sales representative or agent for information on replacing the UV lamp e f the UV lamp burns out E18 is displayed on the digital temperature indicator If this occurs replace the UV lamp When replacing the UV lamp replace the glow starter type FG 7P at the same time Contact a Sanyo sales representative or agent for information on replacing the UV lamp 68 Precautions when using the UV lamp The clauses below are applicable when the model is MCO 18AC UV or an optional UV system kit MCO 18UVS3 is installed NWARNING Do not look directly at UV light UV light is harmful to the eyes Always use the humidifying pan and humidifying pan cover The humidifying pan and cover prevent UV light from escaping Always use them even when not humidifying To check whether the UV lamp is lit open the outer door and then press the door switch with the inner door still closed Visible blue light can be confirmed from the front of the humidifying pan cover UV light is harmfu
32. in the controller 16 Numerical value shift key 4 Pressing this key in the setting mode causes the numerical value to shift In key lock mode pressing this key makes key lock ON or OFF See page 23 for details 17 Digit shift key Db Pressing this key the setting mode causes the changeable digit to shift Pressing this key more than 5 seconds enters key lock mode See page 23 for the key lock Remote alarm terminal 11 The terminal of the remote alarm is installed at the rear right side of the incubator The alarm is outputted from this terminal Contact capacity is DC 30 V 2 A Contact output between COM and N O between COM and N C At normal Open Close At abnormal Close Open Note The alarm is actuated when the power cord is disconnected from the outlet or the power switch is OFF he remote alarm cannot be stopped by pressing the alarm buzzer stop key BUZZER since the remote alarm is not conjunct with the alarm buzzer stop key BUZZER REMOTE ALARM MAX DC30V 2A Remote alarm terminal 60 x INSTALLATION SITE To operate this incubator properly and to obtain maximum performance install the incubator in a location with the following conditions N WARNING Ventilate a room air occasionally when using CO gas for control The gas density will increase in an enclosed small room and high level of gas density can be hazardous to health In addition avoid inhaling the chamber air directly when opening
33. personnel Install the MCO 21GC and then perform the following procedure 1 Connect the CO gas pipes to connecting ports for gas pipes B Connecting ports for CO gas pipes are located on the left back of the incubator Refer to page 8 Note Only port can be used when the MCO 21GC is not installed There will be 2 ports ports and after the MCO 21GC is installed 2 Connect a CO gas cylinder equipped with a gas pressure regulator to each gas pipe Refer to page 14 for information on connecting the CO gas cylinders Gas cylinder A is the gas cylinder connected to the gas pipe connected to port A and gas cylinder is the gas cylinder connected to the gas pipe connected to port B 3 Open the valve of both gas cylinders 4 Check that the gas supply line indicator on the control panel is lit by pressing the CO gas supply line switching key 5 Select a CO gas supply line A or B 6 The MCO 21GC detects that there is no more CO gas in a cylinder when the CO density in the chamber does not increase for a while even after opening the CO gas valve in the incubator It switches the gas supply line when it detects that there is no more gas At that time the following indications of switching the gas cylinder are provided CO Ec E line indicator Digital temperature indicator Empiyline line Theotherline other line 01 is displayed alternately wit
34. ready to use If a function code which is latter than 04 is input without inputting service code and ENT key is pressed a device automatically returns to chamber temperature display and audible alarm sounds for 1 second Service code is stored unless 000 is input in or a main power turns off Display of UV lamp accumulation time Range 0 260 Note It is necessary to select 001 installed F20 beforehand Input 04 on temperature display and press ENT key to display UV lamp accumulation time on display Unit UV lamp accumulation time is written in non volatile memory with every 24 hours UV lamp setting illuminating time is added to accumulation time by the unit of minute When it is less than 5 minutes 5 minutes is added 24 hours actually 4 4 hours are added when a device is set in 24 hours sterilization mode selected 001 in FO2 When UV lamp accumulation time is over 1000 hours UV indicator on control panel will flash Display accumulation time and change to 000 by using and pp key Press ENTER key Action of UV indicator on control panel UV lamp accumulation time When UV lamp illuminates When UV lamp goes off Up to 1000hours Illuminates 1000hours or more Illuminates F05 Operation Display of CO2 sensor input voltage Input F05 on temperature display and press ENT key to show the voltage on sensor on CO display Press ENT key to be
35. the door if CO gas is used Si l appareil est utilis dans un evdroit restreint le niveau de la densite CO de l air peut s lever et peut se 5 so ys CAR 7 1 tre nocif aux humains Evitez d aspirer l air provenant de l in rieur de l appareil quand vous ouverz la porte m A location not subjected to direct sunlight Do not install the incubator under direct sunlight Installation in a location subjected to direct sunlight cannot obtain the intended performance m A location away from heat generating sources Avoid installing the incubator near heat emitting appliances such as a heater or a boiler etc Heat can decrease the intended performance of the incubator 5 C higher than the ambient temperature The chamber temperature must be at least 5 C higher than the ambient temperature For example the chamber temperature is set to 37 C the ambient temperature must be less than 32 C Keep the ambient temperature in adequate range m A location with a sturdy and level floor Always install the incubator on a sturdy and level floor The uneven floor or tilted installation may cause failure or injury Install the incubator in stable condition to avoid the vibration or noise Unstable condition may cause vibration or noise N WARNING Install the incubator on a sturdy floor If the floor is not strong enough or the installation site is not adequate this may result in injury from the incubator falling or tipping over Select
36. 0 Setting of communication parameter Factory default 000 Setting of linkage action between remote alarm and buzzer Factory default 000 Setting of Ring Back time Factory default 030 Display of ROM version Function mode temperature calibration mode F01 Operation Action Input a Function code by pressing gt gt key and key and press ENT key Setting of UV lamp illuminating time Factory default 0 minute 0 30 minutes Note It is necessary to select 001 installed in F20 beforehand Input F01 on temperature display and press ENT key Current setting time appears CO display Input a value by pressing key and gt gt and press ENT key to store the value A device automatically returns to chamber temperature display lf a value deviates from setting range is input and ENT key is pressed audible alarm sounds for a second and a previous setting value remains UV lamp illuminates for predetermined setting time and goes off automatically after a door is open and closed There are 3 5 seconds delay to illuminate UV lamp because water sensor checks water level preceding just after a door is closed UV lamp does not illuminate when a door is open UV lamp goes off if door is open during UV lamp illuminates 219 In chamber temperature display press CAL key for 5seconds to step to FO2 lt Operation gt lt Action gt Note lt
37. Always use sterile distilled water e The humidifying pan is not filled with sterile distilled water Always use sterile distilled water e The door is opened frequently e There is any gas leakage at the connection or pin hole on the tube It is recommended to replace the tube once a year e The gasket of the inner door is not completely sealed e The access port at the upper left corner is opened e The environment around the incubator is not normal The source of the contaminated gas is nearby e The incubator is installed in an enclosed space e HEPA filter is provided in the gas piping If it takes much time to recover the gas density even though the gas pressure is normal it may be that dust on the HEPA filter prevents the gas flow Contact a Sanyo sales representative or agent e The control method for the incubator is the ON OFF method gas is intermittently injected as the gas concentration in the chamber approaches the set value Injections may be stopped for periods of approximately 15 seconds but that is not an error Note If the problem still has not been solved after trying the above checks and countermeasures or for any problems not covered here contact a Sanyo sales representative or agent DISPOSING OF THE CO INCUBATOR When disposing of the CO incubator contact a Sanyo sales representative or agent NWARNING The incubator must be dismantled and disposed of by qualified personnel only If the inc
38. CO display If you press ENT key or there are no key operations for 90seconds a device automatically returns to chamber temperature display This is adjustment value for CO level calibration When actual CO level is 4 5 and CO display is 5 0 if 4 5 is input for zero calibration the adjustment value obtained from calculation is 4 5 5 0 x 100 Default setting 90 12 lt gt Note F13 lt Operation gt F14 lt Operation gt Display of a temperature on AT sensor Input F12 on temperature display and press ENT key to display a temperature on AT sensor on display If you press ENT key or if there are no key operations for 90seconds a device automatically returns to chamber temperature display Temperature on AT sensor is about 5 Chigher than an actual ambient temperature because AT sensor is placed on a PCB in back cover where it is affected by waste heat from electric components Initialization of non volatile memory Factory default 000 This function should be used only when data in the non volatile memory cannot be restored owing to unexpected events ex noise Input F13 on temperature display and press ENT key to display 000 on display Change the 1 digit to 1 and press ENT key to initialize all the data non volatile memory Default settings in non volatile memory Chamber temperature 37 0C CO level 0 0 UV lamp lighti
39. CO2 valve ass y by 8 2 screws See Fig 2 4 Remove the cap and fit 3 CO2 gas pipe connecting port B with 4 Nut and 5 Spring washer 5 Connect the lead wire of valve with the yellow connector in the harness CNO Fig 2 6 Remove the upper tube of valve A Rear cover 7194 By using 2 ass y connect CO valve valve and tube See Fig 3 7 Connect the bottom tube of 7 CO2 valve ass y with 3 2 gas pipe connecting port B 40 8 By using 9 Tube connect 3 CO2 gas pipe connecting port B with CO2 tank B 9 Fix tubes firmly by using 6 Tube band L and 7 Tube band S such as Fig 3 specified 10 Install the rear cover back to the position NOTE Every tubes should be connected tight to prevent gas leakage Fig 3 2 Y pipe ass y CO valve B Tube X 7 Tube band S CO valve A 1 CO2 valve ass y 6 Tube band L 5 4 Nut 5 Spring washer 3 2 gas pipe Yellow K connecting port connector A To CO cylinder A cylinder 4 41 lt Setting of cylinder automatic changeover mode gt Before using MCO 21GC it is necessary to set gas auto changer mode in Function mode Kw nation Turn the power switch ON Current chamber temperature is displayed chamber temperature is Current chamber temperature is displayed Press CAL key for 5 seconds
40. EVEL SENSOR MCO 18AC UV or MCO 18AC with MCO 18UVS3 ALARMS SAFETY AND SELF DIAGNOSIS SETTING OF ALARM RESUME TIME Operation after power failure ROUTINE MAINTENANCE Cleaning the chamber and inner attachments Filling the humidifying pan CALIBRATION Temperature calibration calibration TROUBLESHOOTING DISPOSING OF THE CO INCUBATOR AUTOMATIC CO CYLINDER CHANGEOVER STACKING INCUBATORS SPECIFICATIONS PERFORMANCE SAFETY CHECK SHEET HQ U U U U U U U U U U U U U U U U U U U U U U U N O W N 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 26 P 27 30 U U U UU Uo 31 32 33 34 39 40 42 43 44 INTRODUCTION Read this manual carefully before using the appliance and follow the instructions for safety operation Sanyo never guarantee any safety if the appliance is used for any objects other than intended use used by any procedures other than those mentioned in this manual Keep this manual in an adequate place to refer to it as necessary The contents of the manual will be subjected to change without notice due to the improvement of performance or functions Contact Sanyo sales representative or agent if any page of the manual is lost or page order is incorrect Contact Sanyo sales representative or agent if any point in this manual is unclear or if there are any inaccuracies No part of this manual be reproduced in any form without the expressed
41. MCO 18AC with MCO 18UVS3 19 The clauses below are applicable when the model is MCO 18AC UV or an optional UV system kit MCO 18UVS3 is installed A UV lamp is located inside the duct to sterilize the water in the humidifying pan and air circulating in the chamber Following shows precautions and instructions about the UV lamp When all chamber attachments are installed correctly only the inside of the duct and the inside of the humidifying pan cover are exposed to UV light Correctly install all of the chamber attachments when starting a cell culture and never turn ON the UV lamp when the humidifying pan cover is removed Always install the humidifying pan cover even when using the incubator without turning ON the UV lamp Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity recovery performance The UV lamp stays lit for a preset period of time after the outer door is closed The default setting is for o minutes The period can be changed when necessary as shown in the page 21 e f the outer door is not opened for at least 12 consecutive hours the UV lamp will light for the preset time period every 12 hours The period can be changed when necessary as shown in the page 21 e When checking the UV lamp operation open the outer door and push the door switch with the inner door closed The visible blue light can be checked under the humidifying pan cover The UV light is harmful to
42. Service Manual SANYO CO Incubator FILE No MCO 18AC MCO 18AC UV SANYO Electric Co Ltd Biomedical Business Division SM9910236 Effective models This service manual is effective for following models Product code Voltage and Frequency MCO 18AC 823 632 51 110 120V 82363252 220V _ 823 63253 823 63254 220 240V 823 632 55 110 120V MCO 18AC UV 823 632 71 110 120Vv 602 93652722 502 __823 632 73 2200 602 __823 632 74 220 240V 502 823 632 75 110 120V 60Hz Contents Specifications 7 Structural specifications Control specifications Performance specifications Dimensions CO gas Circuit Temperature calibration CO Span setting CO calibration 7 Components on Connections on PCB Electric parts 7 Wiring diagram 7
43. a level and sturdy floor for installation This precaution will prevent the incubator from tipping Improper installation may result in water spillage or injury from the incubator tipping over m A location not prone to high humidity Install the incubator in the ambient of 80 or less humidity Installation under high humidity may cause current leakage or electric shock N WARNING Do not use the incubator outdoors Current leakage or electric shock may result if the incubator is exposed to rain water Never install the incubator in a humid place or a place where it is likely to be splashed by water Deterioration of the insulation may result which could cause current leakage or electric shock m A location without flammable or corrosive gas Never install the incubator in a flammable or corrosive location This may cause explosion or fire or may result in the current leakage or electric shock by the corrosion of the electrical components 61 12 INSTALLATION 13 1 Remove the packaging materials and tapes Remove all transportation packaging materials and tapes Open the doors and ventilate the incubator If the outside panels are dirty clean them with a neutral detergent and wipe it up with a wet cloth WARNING Do not put the packing plastic bag within reach of children as suffocation may result 2 Adjust the leveling feet Extend the leveling feet by rotating them counterclockwise to contact them to the f
44. al YAMAMOTO lt Picasso has greater capability of clean for rust than Gif gt 21 Operation check after replacing sensor and main P C B Note Ensure that setting values for F01 F06 F07 F08 F21 and F25 should be written down before replacing main PCB 1 Call F03 and input 384 service code 1 Press CAL key for 5 seconds and call by using n key and gt gt key Press ENT key 2 Input 384 and press ENT key 2 Fill distilled water in humidifying pan and set a chamber temperature at 37 C 1 Run a unit for approx 8hours and ensure chamber humidity should be equal or higher than 90 Note Required humidity will be usually obtained after a unit keeps running for approx 5 hours without door opening 2 density should be set at 0 3 Ensure there should be no condensation in the chamber If there is step to FO6 decrease output ratio for bottom heater If there is condensation on an inner door step to and increase output ratio for door heater 4 Measure chamber temperature by using standard thermometer unit 0 1 C or PT100 Q sensor 3 Temperature and 2 calibration Temperature and CO2 calibration should be done in accordance with calibration procedures See Page 6 for Temperature calibration Page 7 for CO2 calibration 28 Parts layout HN Control panel outer door Outer door is open In the chamber Inner wall Circul
45. amber temperature CO level e UV lamp illumination time Setting of serial communication mode Factory default 000 Input 22 on temperature display and press ENT key to display 000 display Change the n digit to set the communication mode Control mode the 3 digit 0 Local Default 1 Remote Baud rate the p digit 0 2400bps Default 1 4800bps 2 9600bps Linkage action between remote alarm and buzzer Factory default 000 Input F24 on temperature display and press ENT key to show 00X on display Change the 15 digit to change linkage action 000 Not linked between remote alarm and buzzer 001 Linked between remote alarm and buzzer Setting of Ring back time Factory default 030 30min Range 0 60 Input F25 on temperature display and press ENT key to set current Ring Back delay time Change the 2 digit to set delay time 000 Not Ring back Display of ROM version Input F30 on temperature display and press ENT key to display current ROM version on CO display Dx 13 14 15 16 17 18 Bottom heater control Purpose Bottom heater is controlled at predetermined time to shorten humidity recovery time When a chamber temperature is within set temperature 0 4 C 0 8 C bottom heater is turned on for 1 minute at longest except for a while a chamber temperature stabilizes after a power was turned on or an outer door leaves open fo
46. ating fan CO2 sensor Sampling port Inner door latch Inner wall Access hole with cap Humidifying pan Humidifying pan CO2 injection pipe CO2 sensor Circulating fan motor Access hole Back side Overheat protect sensor Lead wires for MTR 480 L0 Temp sensor Place to fit PCB for 2 sensor Main PCB glow starter Cover is removed Lower part of back side for MCO 18UVS3 CO2 injection CO2 injection port A port B For MCO 21GC Remote alarm terminal CO2 sensor Switching power supply Main PCB For optional components Power switch Switching power supply Power transformer Noise filter 29 Test data lt Measuring condition gt Ambient temperature 20 C Setting temperature 37 0 C Setting CO2 density 5 0 No load Note These data are the reference only Temperature pull up 40 39 30 Temperature C 25 20 0 1 2 3 4 Time scale Hour Humidity pull up 100 90 80 70 60 Humidity Rh 50 40 0 1 2 3 4 Time scale Hour 30 Temperature change during power failure 40 2 35 D a 30 D 25 20 0 1 2 2 4 Time scale Hour Temperature recovery 38 Door open for 30sec 37 m m wm m mm
47. ating fan UV lamp and electromagnetic valve for CO when door is open 14 Humidifying pan Use sterile distilled water to fill the pan MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed Install the pan properly so that it can be covered with the pan cover 15 Remote alarm terminal Refer to page 11 16 Access port When not in use cap with the silicon caps on both outside and inside 17 Power switch Main switch of the incubator Also functions as an over current breaker The switch is covered by a switch cover to prevent the accidental push To turn on or off the switch remove the switch cover by loosening the screw 18 Humidifying pan cover Prevents UV light being exposed to the chamber When filling the pan lift the front side and take out the pan See page 30 for details 19 UV lamp Sanyo UV lamp does not generate ozone Never look at the UV light directly For replacement contact Sanyo sales representative or agent 20 Water level sensor for humidifying Detects the water level in the humidifying pan See page 24 for details 21 Glow starter For UV lamp model FG 7P MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed 58 Control panel and keypad UV INJECT DOOR 7 OVER HEAT TEMPERATURE SANYO E TR CO INCUBATOR gt 4 ar T 17 16 15 WD 10 11 12 13 14 1 Digital temperature indi
48. ber but this does not indicate a problem 66 SETTING THE CHAMBER TEMPERATURE AND CO DENSITY Table below shows the basic procedure for setting the chamber temperature and CO density The upper limit alarm temperature setting is also shown in the table Perform key operations in the sequence indicated in the table The example in the table is based on the assumption that the desired temperature is 37 C and density is 5 Adjustment of the upper limit regulator should be executed after the chamber temperature reaches the stable condition Note incubator is set at the factory so that the chamber temperature is 37 C and CO density is 0 When starting the incubator for the first time or after not using it for an extended period of time allow at least 8 hours for the chamber temperature humidity and sensor to become stable after setting the desired chamber temperature at 0 density Then change the setting to the desired density Basic operation sequence Example Chamber temperature 33 C density 5 NE Description of operation Key operated Indication after operation The current chamber temperature is displayed 1 Turn the power switch ON in the digital temperature indicator Press set key ser The left digit blinks B wal hift When pressed ressing numerical value shi dd the changeable digit is shifted key and digit shift key Wh d en pressed set the figure to
49. cancel Key Lock prior to commence calibration lt Prior to commence CO zero calibration gt 1 Ensure CO is not present and the humidity is stabilized in the chamber 2 Ensure CO zero calibration should be performed before CO is injected 3 When zero calibration is performed again leave the outer door open to ensure is not present in the chamber lt Procedure gt 1 Fill distilled water in a humidifying pan and set a temperature at 37 C and set density at O 2 Run a unit for approx 8hours until chamber humidity can reach 90 or higher Note Do not open an outer door until chamber humidity is stabilized Chamber humidity will be stabilized after unit is running for approx Shours if an outer door is not opened When chamber temperature is displayed and press CAL key for approx 5seconds to blink the 3 digit on temperature display Press 4 key to display F in the 3 digit Press P key to display O in the 15 digit and 3 in the 1 decimal digit is displayed on temperature display Press ENT key to display 000 on display Change the value to 384 by using key and pp key Press ENT key to step to Function mode If there is condensation in a chamber step to F06 by using 4 key and pp key Current output ratio for humidifying heater is displayed on CO display Change the ratio to lower value not to generate condensation Ex When current output ratio for hu
50. cator TEMPERATURE C Normally this indicator shows the chamber temperature In the setting mode it shows the set value of the chamber temperature diagnostic function detects any abnormality an error code will be displayed 2 Heater lamp HEAT This lamp lights when the heater is energized If the self 3 UV indicator UV This lamp lights when the UV lamp is The blink of this indicator recommends the replacement of UV lamp See page 19 for the details MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed 4 Door lamp DOOR This lamp lights when the outer door is open 5 Water level alarm lamp RH PAN This lamp blinks when the water in the humidifying pan is less than approximately 0 8 liter MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed 6 Upper limit regulator This regulator is used to set the upper temperature limit 7 Over heat lamp OVER HEAT This lamp lights when the chamber temperature reaches the upper limit set value 8 CO inject lamp INJECT This lamp lights when CO gas is being injected 9 Digital CO density indicator Normally this indicator shows the CO concentration the chamber In the setting mode it indicates the set value of the concentration mode the decimal point in the digital density indicator blinks 10 Set key SET Pressing this key to enter the setting mode and the digits to be set will blink I
51. d by 0 196 in a minute when CO valve is open 01 and chamber temperature are displayed alternately audible alarm sounds intermittently The diagnosis that cylinder becomes empty is done two times therefore it takes approximately 2minutes to display an error code after the cylinder becomes empty E05 E06 Temp sensor is open short circuited When a temperature in temp sensor is lower than 0 diagnosis is given that temp sensor is open circuited displaying E05 alternately with chamber temperature Audible alarm sounds intermittently When a temperature in temp sensor is higher than 60 C diagnosis is given that temp sensor is short circuited displaying E06 alternately with chamber Note temperature Audible alarm sounds intermittently In E05 and E06 displayed temperature is different from products actual temperature E09 E10 AT sensor is open short circuited When a temperature AT sensor is lower than OC diagnosis is given that AT sensor is open circuited displaying E09 alternately with chamber temperature Audible alarm sounds intermittently When a temperature in AT sensor is higher than 60 C diagnosis is given that AT sensor is short circuited displaying E10 alternately with chamber temperature Audible alarm sounds intermittently Note In E09 and E10 displayed temperature is different from products actual temperature E11 sensor output voltage is abnormal When
52. e door hinge from left to right or vice versa 2 Inner door Made of tempered glass however avoid excessive impact on the glass 3 Leveling foot Screw type for adjusting the height Adjust the foot so that the incubator can be level 4 Tray be pulled toward you 5 Tray support be removed by lifting the front side and pulling toward you 6 Side support Right and left side supports can be removed for disinfection See page 27 28 and 29 7 Fan cover Inlet of circulating air Removable 8 Duct Flow path for circulating air Removable 9 Fan inside the rear duct Made from polypropylene resin Can be disinfected by an autoclave 10 Sample air outlet This also functions as an internal gas outlet Normally cover this outlet with the sample air outlet cap 11 Sample air outlet cap Always attach this cap except at the time of using of sample air outlet Note Operation without sample air outlet cap may result low humidity or condensation on the outer door 12 Connecting port for gas pipe When an optional component MCO 21GC automatic cylinder changeover system is installed both A and B are available If MCO 21GC is not used only A is available Refer to page 14 for gas cylinder connection Ensure that the gas pressure is set at 0 03 MPaG 0 3 kgf cm G 4 3 psiG Refer to page 39 for automatic CO cylinder changeover system 13 Door switch Detects the door opening closing and stops the circul
53. e setting cannot be changed during power failure e The remote alarm during power failure cannot be stopped e The set mode returns to the temperature display mode automatically when 90 seconds has passed without any key operation In this case any setting before pressing enter key is not memorized Pressing calibration key CAL for about 5 seconds leads the calibration mode In the calibration mode the calibration of temperature and density is possible Wrong key operation affects the basic performance Never touch any other keys on the control panel in the event of pressing calibration key CAL accidentally After about 90 seconds the incubator returns to chamber temperature display mode automatically Operation after power failure The set value is memorized by nonvolatile memory Accordingly the incubator resumes the operation with setting before power failure 75 26 ROUTINE MAINTENANCE Cleaning the chamber and inner attachments When the chamber of the incubator is contaminated the chamber and internal attachments should be cleaned and sterilized as follows A WARNING Before performing any repairs or maintenance turn OFF the power switch and unplug the incubator Failure to do say may result in electric shock or injury Be careful not to inhale chemicals vapors or aerosols when cleaning the incubator Doing so be harmful to health NCAUTION Wear rubber gloves when performing maintena
54. eration gt Display of adjustment value for temperature zero calibration Factory default 0 8 C Input F15 on temperature display and press ENT key to display an adjustment value for temperature zero calibration CO display If you press ENT key or if there no key operations for 90seconds a device automatically returns to chamber temperature display When 37 0 is displayed and actual temperature is 37 8 if you input 37 8 as temperature calibration 0 8 will be stored in F15 Press ENT key to display the value on CO display Optional setting of UV lamp water sensor Factory default 000 Input F20 on temperature display and press ENT key to display 000 on CO display If there are no key operations for 90 seconds a device automatically returns to chamber temperature display 000 Uninstalled 001 Installed Setting of serial communication ID Factory default 000 000 255 Input F21 on temperature display and press ENT key to display 000 display Input a value serial communication ID numbers which identifies a product and press ENT key to store serial communication ID 000 Not communicated Collection data sending from apparatus to PC Chamber temperature CO level UV lamp illuminating time AT sensor temperature Door open time Water level in humidifying pan UV lamp accumulation time Setting data sending from PC to apparatus Ch
55. gh the sample air outlet on the inner door 5 Press the calibration key CAL for about 5 seconds again to enter the temperature calibration input mode 6 The left digit on the digital temperature indicator blinks and the digital density indicator goes out 7 Press the calibration key CAL once again to enter the calibration input mode 8 The left digit on the digital density indicator blinks and the temperature indicator goes out 9 Set the present correct density with the digit shift key Pb and numerical value shift key 4 then press the enter key ENT 10 Replace the sample air outlet cap 11 The incubator automatically reverts to the display mode Note This calibration is available when the setting of density is 2 or more Example For an internal density of 5 0 setting and a measured value of 4 5 1 Press the calibration key CAL once 2 Press the calibration key CAL for about 5 seconds again 3 The 3 on the digital temperature indicator blinks and the digital density indicator goes out 4 Press the calibration key CAL once again 5 The 0 on the digital CO density indicator blinks and the digital temperature indicator goes out The left digit on the digital density indicator blinks the temperature indicator goes out 6 Set the present correct CO density 4 5 with the digit shift key bb and numerical value shift key
56. h the temperature on the digital Intermittent tone Blinks Lights temperature indicator To silence the buzzer press the alarm buzzer stop key BUZZER The alarm condition is cleared and normal operation continues 7 When gas cylinder A is switched to B remove gas cylinder A and replace it with new one Note Be careful when handling the empty CO gas cylinder Some gas may still be left in the cylinder 8 When gas cylinder B becomes empty the line is switched to gas cylinder A again The MCO 21GC detects that there is no more gas in a cylinder when the density in the chamber does not increase for a while even after opening the CO gas valve in the incubator The supply line may also be switched for other reasons even though there is gas left in the cylinder blocking or restricting of gas tube reduction of gas pressure or improper opening of CO gas cylinder Always check the gas quantity in the cylinder before disconnecting it 39 88 INCUBATORS Use the following procedure to stack incubators This work is potentially dangerous so contact Sanyo sales representative or agent NCAUTION e Select a floor that is strong enough to support the stacked incubators e Never stack three or more incubators Doing so is dangerous 1 Take out two stacking plates A two stacking plates B four protective stickers and four screws from accessory bag Note When stacking two incubato
57. he water level Therefore the water level alarm lamp may blink a few times after the outer door is closed with the humidifying pan filled sufficiently NCAUTION The foreign particles on the water surface adhere to the water level sensor and its fittings by the capillary action because the sensor is always in the water The adhered foreign particles degrade the sensor performance and the water level alarm lamp may blink with the enough water in the humidifying pan At the cleaning take care not transmit the excessive force to the sensor lead wire ALARMS SAFETY AND SELF DIAGNOSIS The incubator supports the following alarms safety functions and self diagnostic functions Alarms safety and self diagnosis for culture operations Alarm or safety Conditions function Indication Over heat lamp lights If the chamber temperature exceeds Upper limit the upper limit alarm temperature set temperature alarm value If the chamber temperature deviates from the set temperature by 1 C or more If the chamber density deviates from the set value by 1 or more Automatic set temperature alarm Automatic set density alarm Door check The outer door is open When there is no key pressing in each setting mode for 90 seconds When the key lock is ON Auto return Key lock gas cylinder empty If the density does not increase when the gas valve is opened Whe
58. idifying If the water the humidifying is Water level alarm lamp RH fe Water about 0 8 liter PAN blinks UV lamp failure When the UV lamp is burned out E18 is displayed alternately with the temperature on the digital temperature indicator Intermittent tone Remote alarm Recommendation of The accumulated ON time is over about UV indicator blinks ee new UV lamp 1 000 hrs when UV lamp is OFF MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed alarm canceled by pressing the alarm buzzer stop key BUZZER but the remote alarm cannot be stopped And the upper limit temperature alarm cannot be silenced with the alarm buzzer stop key BUZZER 01 is cleared automatically when the gas is connected correctly and the buzzer is silenced with the alarm buzzer stop key BUZZER after changeover of gas supply line When MCO 21GC is installed press the alarm buzzer stop key BUZZER to silence the alarm f one of E05 to E18 except for E12 E13 and E14 is displayed contact a Sanyo sales representative or agent 25 74 SETTING OF ALARM RESUME TIME The alarm buzzer is silenced by pressing alarm buzzer stop key BUZZER on the control panel during alarm condition The remote alarm is not stopped The buzzer will be activated again after certain suspension if the alarm condition is continued The remote alarm continues The suspension time ca
59. ill be blank and CO level is uncontrollable 2 alarm When CO level deviates from setting value 196 CO display will flash and audible alarm sounds and remote alarm outputs after 15 minutes later Overheat protection temperature check mode Setting range 35 C 51C Setting Press SET key three times to display the overheat protection temperature method on temperature display and HI display Turn the volume 101 to adjust overheat protection temperature Press SET key again to return to chamber temperature display 15 Alarm safety mode and self diagnosis When malfunctions related sensors and CO control are occurred an error code and chamber temperature are displayed alternately Note The remote alarm is controlled every minute exceptionally in Auto Zero performs so there are 4 minutes of delay at maximum after an error code is displayed lt Error code gt E01 cylinder becomes empty E05 Temp sensor is open circuited E06 Temp sensor is short circuited E09 AT sensor is open circuited E10 AT sensor is short circuited E11 sensor output voltage is abnormal E12 Main heater is abnormal E13 Bottom heater is abnormal E14 Door heater is abnormal E16 SSR for each heater is open circuit E18 Malfunctions related UV lamp bulb blown out a glow lamp missing etc The following are descriptions of each error codes E01 CO cylinder becomes empty If CO level is not increase
60. ill emit and a device returns to chamber temperature display When you change a value over than 1 0 C it is necessary to calibrate temperature step by step Notes when the unit installation When chamber temperature and humidity are not stabilized just after unit starting there may be case that density on display is higher than actual density It is not abnormal because sensor is heat conducting type Set chamber temperature and set to 0 pour 1 5L distilled water into the humidifying pan Start and run the unit for approx 8hours until chamber temperature and humidity will be stabilized Ensure to commence zero and span calibration after chamber temperature and humidity stabilized Span setting Note Ensure to cancel Key Lock prior to commence calibration Perform CO calibration after you ensure chamber temperature and humidity are stabilized 1 Press CAL key 2 unit steps into CO calibration pre conditioning mode Circulating fan will activate if an outer door is open Dot on CO display will blink 3 Press CAL key for approx 5 seconds 4 The Be digit on temperature display is flashing other digits will go off 6 The 3 digit on CO display is flashing other digit will go off calibration input mode 5 Press CAL key again 7 Input a proper CO density by using Bb key and amp key and press ENT key Example If CO se
61. indicates an earth This symbol means for a power switch This symbol means OFF for a power switch 09 6 ENVIRONMENTAL CONDITIONS This equipment is designed to be safe at least under the following conditions based on the IEC 61010 1 Indoor use m Altitude up to 2000 m m Ambient temperature 5 C to 40 C m Maximum relative humidity 80 for temperature up to 31 C decreasing linearly to 50 relative humidity at 40 C m Mains supply voltage fluctuations not to exceed 10 of the nominal voltage m Other supply voltage fluctuations as stated by the manufacturer m Transient overvoltages according to Installation Categories Overvoltage Categories Il For mains supply the minimum and normal category is m Pollution degree 2 in accordance with IEC 60664 COMPONENTS Handles 5 13 7 8 9 inside md I j EM 545 A x me N ANG T Handles 4 10 11 14 For MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed Rear Right Side 57 i NH 1 19 20 inside For MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed 18 I D 1 Rear Left Side 1 Outer door Sticks to frame with magnetic seal Door heater is installed the outer door panel The door opening is reversible Contact Sanyo representative or agent to change th
62. infectant alcohol 2 Clean and wipe off inside the chamber with disinfectant alcohol 3 Set the CO density to 0 and set the UV 24 hour lighting mode a Description of operation Key operated Indication after operation _ The left digit in the temperature Press calibration key for 5 seconds blinks By pressing numerical value When pressed the When pressed the figure of settable digit changes of settable digit changes 2 key and digit shift key When pressed set the figure to F02 the changeable digit is shifted 000 is displayed Press enter key in the digital CO density indicator By pressing numerical value shift When pressed key set the figure to 001 the figure of settable digit changes Set value is memorized and the display returns to 5 Press enter key normal display mode This procedure must preformed only with the outer door closed and the UV lamp turned OFF UV lamp will light continuously for 24 hours after this parameter is set The setting is canceled if the outer door is opened Perform the above procedure to set the 24 hours lighting mode again when opening the outer door incubator keeps running with set temperature and CO density during UV lighting Install the attachments after completion of the 24 hour lighting mode Note Set the upper limit alarm temperature to at least 10 C higher tha
63. ing may cause electric shock in the case of an incomplete circuit Connect the unit to a power source as indicated on the rating label attached to the unit Use of any other voltage or frequency other than that on the rating label may cause fire or electric shock S Never store volatile or flammable substances in this unit if the container cannot be sealed These may cause explosion or fire Do not insert metal objects such as a pin or a wire into any vent gap or any outlet on the unit This may cause electric shock or injury by accidental contact with moving parts Use this unit in safe area when treating the poison harmful or radiate articles Improper use cause bad effect your health or environment Turn off the power switch if provided and disconnect the power supply to the unit prior to any repair or maintenance of the unit in order to prevent electric shock or injury Do not touch any electrical parts such as power supply plug or operate switches with a wet hand This may cause electric shock sb NWARNING Ensure you do not inhale or consume medication or aerosols from around the unit at the time of maintenance These may be harmful to your health D Never splash water directly onto the unit as this may cause electric shock or short circuit Never put containers with liquid on the unit as this may cause electric shock or short circuit when the liquid is spilled Never bind process
64. l to the eyes so do not light the UV lamp when the inner door or humidifying pan cover is open Be careful when handling the UV lamp There is a UV lamp inside of the duct Be careful not to damage it when installing or removing chamber attachments or the humidifying pan 69 20 Setting the UV lamp period The clauses below are applicable when the model is MCO 18AC UV or an optional UV system kit MCO 18UVS3 is installed Follow the procedure below when changing the setting of the UV lamp ON period Basic operation sequence Example change of UV lamp ON period from 5 minutes to 3 minutes Description of operation Key operated Indication after operation Press calibration key The left digit in the digital for 5 seconds temperature indicator is blinked By pressing numerical value shift 2 key and digit shift key set the figure to 01 When pressed the changeable digit is shifted The current setting is displayed Press enter key ENT o in the digital CO density indicator By pressing numerical value shift When pressed the figure of 4 key and digit shift key settable digit changes set the figure to 003 Set value is memorized and the display return to Press enter key ENT normal display mode e The UV timer can be set from 0 to 30 min The default setting is for 5 min When the UV timer is set to zero the UV lamp will not light e f the outer door is opened
65. llowing procedure Procedure 1 Open and close the outer door with the humidifying pan empty Check that RH PAN indicator on the control panel blinks after a few seconds Open and close the outer door with the humidifying pan full Check that RH PAN indicator on the control panel is off after a few seconds NOTE Set the humidifying pan cover properly when checking operation of the water level sensor Also close the inner door completely The UV lamp is on after operation of the water level sensor when the UV lamp ON period is set Never loock at the UV light directly 39 Automatic CO cylinder changeover system MCO 21GC lt Parts gt 3 gas pipe amp 7 Tube band S connecting port B 1 CO valve ass y 4 Nut 8 Screw A 0 5 Spring washer 9 Tube 2 Y pipe ass y 6 Tube band 1 10 Tube 1100 lt Preparation gt U o 1 Remove the tube band on the 7 valve ass y e T and remove the hose and the capillary 1 CO valve ass y 2 Fit 10 tube L100 by the tube band which was removed in procedure 1 Make sure the tube band is fixed firmly to prevent gas leakage Hold both edges Remove the tube and by the tube bands capillary Fig 1 lt Installation gt 1 Disconnect the power cable and make sure that the unit is not supplied with the power 2 Loosen and remove 6screws and remove the rear cover 3 Fit 1
66. loor Ensure the incubator is level 3 Fix the incubator Two fixtures are attached to the rear of the frame Fix the frame to the wall with these hooks and or chain 4 Ground earth WARNING Use a power supply outlet with ground earth to prevent electric shock If the power supply outlet is not grounded it is necessary to install a ground by qualified engineers Never ground the incubator through a gas pipe water main telephone line or lightning rod Such grounding may cause electric shock in the case of an incomplete circuit e Stacked module When the product is used in stacked module make sure to fix the incubator by using the stacking kit WARNING Do not climb onto the incubator and do not put articles on the incubator This may cause injury by tipping or damage to the incubator When stacking the incubator follow the procedure shown on page 40 e If not used When the incubator is not used dispose the water in the humidifying pan and remove any moisture in the chamber completely Check that the chamber is completely dry before closing the doors e Before moving the incubator Empty the humidifying pan completely before moving the incubator Spilled or splashed water cause current leakage or electric shock 62 Connection of CO gas cylinder A WARNING Check the gas type and ensure that it is fit for the purpose Make sure that all pipes are connected correctly and are not liable to
67. mage of gasket Also before opening the inner door check that the UV light is OFF MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed e Take care of the inside of the outer door It may get hot e Never lean or press on the glass Broken glass due to intentional force may cause injury e Alarm Always investigate the cause and fix the alarm condition immediately when the alarm is activated Refer to page 25 for alarm details e Fix the tray supports and trays securely Incomplete installation may cause injury or damage 65 16 START UP OF INCUBATOR 17 When start the test operation or the operation follow these steps as below 1 Install the incubator referring to INSTALLATION on page 13 2 Remove all transportation packaging materials and tapes on the attachments in the chamber Then clean and sterilize the chamber and inner attachments Refer to Cleaning the chamber and inner attachments on page 27 3 Add approximately 1 5 L of sterile distilled water to the humidifying pan Refer to page 30 4 Turn ON the power switch on the rear left side Note The humidity in the incubator chamber is adjusted to the optimum setting To prevent condensation on the surface inside chamber and the inner door there is a low temperature area under the humidifying pan in the bottom of chamber to recondense evaporated moisture Condensation may occur around the humidifying pan at the bottom of the cham
68. may result 54 NCAUTION Use a dedicated power source a dedicated circuit with a breaker as indicated on the rating label attached to the unit branched circuit may cause fire resulting from abnormal heating Connect the power supply plug to the power source firmly after removing the dust on the plug A dusty plug or improper insertion may cause a heat or ignition Q Never store corrosive substances such as acid or alkali in this unit if the container cannot be sealed These may cause corrosion of inner components or electric parts oO Check the setting when starting up of operation after power failure or turning off of power switch The stored items may be damaged due to the change of setting o Be careful not to tip over the unit during movement to prevent damage or injury Prepare a safety check sheet when you request any repair or maintenance for the safety of service eo personnel LABELS ON THE INCUBATORS Warning and caution labels are attached to the incubator The following table describes the labels This label is attached to covers that access high voltage electrical components to prevent electric shock Only a qualified engineer or service personnel should be allowed to open these covers This symbol indicates an ultraviolet light UV caution This symbol indicates that caution is required Refer to product documentation for details EP Vw This symbol indicates a hot surface This symbol
69. midifying heater is 005 change the value to 004 or 003 Note Ensure to change output ratio after wiping drips off If there is condensation on inner door glass step to FO7 by using 4 key and pp key Current output ratio for door heater is displayed display Change the ratio to higher not to generate condensation When current output ratio for door heater is 005 change the value to 006 or 007 Note Ensure to change output ratio after wiping drips off If a displayed chamber temperature does not match with setting temperature commence temperature calibration Ex When displayed temperature is 37 0 C and actual chamber temperature is 36 7 C press CAL key for approx 5seconds Change the value to 36 7 by using key and p key Press ENT key to finish temperature calibration CO zero calibration Press CAL key for approx 5seconds to blink 3 digit on temperature display Press CAL key again to blink digits on display Change digits to 00 0 by using key and pp key Press ENT key to finish CO zero calibration Note Ensure to change service code from 384 to 000 after zero calibration is finished Components on Descriptions marked with are optional components CN9 1 2 CO valve 3 4 CO auto changer valve 1 122 O DPio2 SES CN14 J104 444 O Po nos 9 1 2 UV lamp relay RH PAN 4 J106 A
70. n be set by following the procedure shown in the table below The example in the table is based on the assumption that the desired duration is 20 minutes Note The duration is set in 30 minutes at the factory Table Changing procedure for alarm resume time Ex change from 30 minutes to 20 minutes E Description of operation Key operated Indication after operation The current chamber temperature is J 1 displayed Press calibration ke 2 CAL The left digit blinks 9 10 5 seconds et the figure to o with the numerical value shift key o the figure of settable digit changes and digit shift key The settable digit is shifted FE 5 The current setting is displayed 4 P ter key ENT 4 pressenerkey ENT The middle digit blinks El Set the figure to 020 with the When pressed 2 numerical value shift key the figure of settable digit changes The setting is memorized and Press enter key the current chamber temperature is J displayed e The settable alarm resume time are 0 10 20 30 40 50 or 60 minutes The setting is 000 010 020 030 040 050 or 060 respectively The buzzer would not reset if the reset time is set in 000 e It is recommended to set the alarm resume time when the incubator is not under alarm condition The setting during alarm condition is effective on the next alarm condition e Th
71. n the calibration 11 Calibration key CAL Pressing this key to enter the CO calibration pre conditioning mode Normally when the outer door is opened the fan motor will stop operating This mode allows the fan motor to continue to circulate the chamber environment to ensure accurate CO uniformity inside the chamber At this time by pressing this key for approximately 5 seconds the incubator enters the temperature calibration input mode And then pressing this key again the incubator enters the calibration input mode See page 31 and 32 for the details Also used to change the UV lamp ON period See page 21 for the details Note This operation affects the basic performance When calibrating the density contact a Sanyo sales representative or agent 59 10 12 Alarm buzzer stop key BUZZER Press this key to silence the buzzer when the alarm operates and the buzzer sounds The remote alarm is not stopped 13 gas supply line indicator The lamp for the supply line currently in use lights up provided that MCO 21GC automatic CO cylinder changeover system option is installed 14 gas supply line switching key This key to select gas supply line is available only when an automatic CO cylinder changeover system MCO 21GC option is installed When one CO cylinder is empty the CO is supplied by the other cylinder automatically 15 Enter key ENT Pressing this key memorizes the set value
72. n the chamber set temperature when using the 24 hour lighting mode of UV lamp The 24 hour lighting mode may cause an automatic chamber temperature alarm because the temperature of the chamber will increase Pressing calibration key CAL for about 5 seconds leads the calibration mode In the calibration mode the calibration of temperature and density is possible Wrong key operation affects the basic performance Never touch any other keys on the control panel in the event of pressing calibration key CAL accidentally After about 90 seconds the incubator returns to chamber temperature display mode automatically 1 22 KEY LOCK FUNCTION This incubator is provided with a key lock function When the key lock is ON change of temperature or density setting through the key pad is not available Note The key lock is set in OFF mode LO at the factory DE Key lock is OFF Enable to change of temperature and CO setting Key lock is ON Disable to change of temperature or CO setting Procedure for lock settina change from kev lock OFF to key lock Description of operation Key operated Indication after operation The current chamber temperature and CO density are displayed LO is displayed in the temperature Press digit shift key for 5 seconds indicator Press numerical value shift key When pressed the figure of settable and scroll
73. n the gas supply line is switched Gas line changeover only when MCO 21GC is installed If the temperature sensor is Chamber disconnected temperature sensor abnormality If the temperature sensor is short circuited If the ambient temperature sensor is disconnected Ambient temperature sensor abnormality If the ambient temperature sensor is short circuited CO sensor abnormality If the output voltage of the CO sensor is abnormal If the upper limit alarm temperature alarm operates or if the main heater is open circuit or the main heater relay short circuit Main heater abnormality Bottom heater abnormality If the bottom heater goes open circuit or the bottom heater relay short circuit Door heater abnormality If the door heater goes open circuit or the door heater relay short circuit Disconnection of sensor for each heater If the relay of main heater bottom heater or sensor box heater goes open circuit E12 or E16 and chamber temperature are displayed alternately digits on the temperature indicator blink Door lamp lights Normal display mode E Ss E01 is displayed alternately with the temperature on the digital temperature indicator E01 is displayed alternately with the temperature on the digital temperature indicator gas supply line indicator of empty blinks E05 is displayed alternately with the temperature on the digital temperature indica
74. nable a device to enter calibration measuring mode Circulating fan will not stop rotating during this mode if an outer door is opened Dot on the display will blink Automatically finish CO calibration measuring mode after 5 minutes elapse and buzzer sounds for 3 times When a chamber temperature is lower than set temperature 3 C CO calibration measuring mode will also come to end If CAL key is pressed during CO2 calibration measuring mode a device will perform this mode for another 5 minutes from the beginning Function mode F30 How to access to Setting of UV lamp illuminating time Factory default 000 Setting of UV lamp 24hours sterilization mode Input of Service code Service code 384 Display of UV lamp accumulation time Display of sensor input voltage Setting of output ratio on bottom heater Factory default 7 Setting of output ratio on door heater Factory default 5 Setting of Auto changer mode and DEMO mode Factory default 000 Display of adjustment value for zero calibration Display of adjustment value for span calibration Display of temperature on AT sensor Initialize of non volatile memory Performance check of LEDs and drivers Display of adjustment value for temperature zero calibration Factory default 008 Setting of optional components UV lamp water sensor Factory default 000 Setting of address ID for communication device Factory default 00
75. nce on the chamber Failure to wear gloves may result in cuts or abrasions from sharp edges or corners Note Be careful not to damage the water level sensor or the UV lamp in the chamber duct if the MCO 18AC UV or an optional UV system kit MCO 18UVS3 is installed Do not use detergents or antiseptic solutions with acid alkali or chlorine Doing so may cause discoloration corrosion or rusting 1 Close the valve of the gas cylinder and turn off the power to the incubator 2 Open the outer door Remove 2 pins fixing the inner door hinge and remove the inner door 3 Pull out the all trays See Fig 1 4 Lift the front of the tray supports and pull them out See Fig 2 5 MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed Lift the humidifying pan cover off from the pins on the rear side See Fig 3 Humidifying pan cover 27 76 6 Pull out the humidifying pan See Fig 4 7 Loosen the two screws securing the fan cover and take off the fan cover See Fig 5 8 Lift the duct and remove it from the pins on the rear side See Fig 6 Fan cover Humidifying pan Fig 4 9 Remove the fan by pulling out the central spring and then by pulling out the fan See Fig 7 10 Remove the screw securing the clamp for the side support and remove the clamp See Fig 8 11 Lift the side support off of the pins See Fig 9 Side support
76. ng of water level sensor connection is needed when using the water level sensor option Refer to the following procedure Description of operation Key Indication after operation operated 1 i 3 Turn the power switch ON The current chamber temperature is displayed Press CAL key for 5 seconds The left digit in the temperature indicator is flashed By pressing bb key 4 key set the figure to F20 dll 5 By pressing bb key and 4 key set the figure to 001 optional 4 Press ENT key In density indicator ENT 000 is displayed and the right digit is flashed connection Press ENT key The setting of water level sensor connection is memorized and the current chamber temperature is displayed NOTE water level sensor will not activate even if the water level sensor option is connected when the F20 is set to 000 In the procedure 2 above pressing CAL key for 5 seconds causes the calibration mode In this mode itis possible to calibrate the temperature and CO density and miss operation of the key may affect the basic performance of the unit Take care of the key operation Even if the key is operated wrongly the unit returns to the current value display mode automatically when no key is operated for 90 seconds n this case the setting not entered by pressing ENT key is not effective 16 Check that the water level sensor operates Check the operation of water level sensor by the fo
77. ng time F01 0 minute UV lamp accumulation rate 04 0 Balance value of humidifying heater 06 Balance value of door heater 07 5 Data for CO zero calibration 10 000 Data for CO span calibration F11 100 Data for temperature zero calibration F15 008 Optional setting of UV lamp water sensor F20 Uninstalled 0 Communication ID F21 0 Communication mode F22 0 Linkage action between remote alarm buzzer Not linked 0 F24 Ring back 30 minutes Key Lock data OFF Auto Changer data the 37 digit in F08 OFF Demo Mode data the 2 digit in F08 OFF Input F13 on temperature display and press ENT key to display 000 on CO display Change the 1 digit to 2 and press ENT key to initialize following data in non volatile memory Data for CO zero calibration F10 000 Data for CO span calibration F11 100 Note Service code should not be initialized Check of LEDs and drivers performance Input 14 on temperature display and press ENT key to flash lamps and the digits and buzzer sounds with 0 5seconds intervals Press ENT key to stop action If there are no key operations for 90seconds a device automatically returns to chamber temperature display 205 F15 lt Operation gt Ex F20 lt Operation gt F21 lt Operation gt F22 lt Operation gt F24 lt Direction for use gt F25 lt Operation gt F30 lt Op
78. o be changed 10 Calibration Temperature In chamber temperature display press CAL key for 5seconds to step to calibration temperature calibration mode Input a proper value by pressing key pp key Press ENT key to store the value and a device automatically returns to chamber temperature display zero In chamber display press CAL key for 5seconds to step to temperature calibration calibration mode and press CAL key again to step to CO calibration mode Input 000 by pressing and gt Press ENT key to recognize and memorize the value as zero calibration device automatically returns to chamber temperature display CO span In chamber display press CAL key for 5seconds to step to temperature calibration calibration mode and press CAL key again to step to span calibration mode Input a proper value by pressing Al key and Pp key Press ENT key to recognize and memorize the value as span calibration A device automatically returns to chamber temperature display When is less than 0 2 on the display input value will be cancelled with an error tone emits and a device automatically returns to chamber temperature display 18 11 12 CO calibrat In CO calibration measuring mode CO density should be measured through sample port ion measuring mode at inner door When a chamber temperature is stabilized within set temperature 3 C press CAL key to e
79. o stop audible alarm sounding Alarming condition Remote alarm action remains on control panel Recovery from alarming Stand by for alarm condition Unit status Audible alarm sounds again Audible alarm does not sound after 30 minutes elapse when again when BUZZER key is Alarming condition remains BUZZER key is pressed pressed if a unit remains after setting time elapse alarming condition Remote alarm alarming display is active 204 19 20 21 Ring back time Setting value Ring back time after audible alarm is cancelled by pressing BUZZER key Not Ring back 00 Not Ringback 20 minutes 40 minutes 060 60 minutes Linkage between buzzer and remote alarm For service use only Remote alarm action Not linked with buzzer Linked with buzzer F24 000 F24 001 Ring back setting F25 030 30min E sounds again e sounds again Factory rig e Remote alarm remains Remote alarm is active active again F25 000 e Buzzer does not sound Buzzer does not sound Not ring back again again e Remote alarm remains Remote alarm is not active active again Temperature offset Purpose Add 0 8C to calibrate a gap between temperature on temperature sensor and 1 2H temperature Water sensor action Note Water sensor is optional components It is necessary to select 001 installed 20 beforehand Action It diagnoses that there is water in humidifying
80. omatic CO level alarm When CO level deviates from set level 1 0 all digits in CO display will flash and audible alarm sounds intermittently in 15 minutes later 17 Door alarm When a door is open DOOR lamp will illuminate At the time fan motor CO2 valve will not be active When a door leaves open for 60 seconds fan motor CO2 valve heater and UV lamp will not be active When a door is closed DOOR lamp will go off 7 Key Lock mode How to set In chamber temperature display press for 5 seconds to step to Key Lock mode When a value is set 11 by pressing Key Lock is set When value is set in LO by pressing Key Lock is unset Press SET key to memorize the value automatically returns to chamber temperature display Note In Key Lock mode the device can step to setting mode but a setting value cannot be changed The device also cannot step to Function mode 8 Automatic UV lamp illumination Purpose For the prevention of contamination UV lamp will illuminate for predetermined time which is set in F01 if a door is closed for 12 hours Note UV lamp is optional component It is necessary to select 001 UV lamp is installed in F20 beforehand 9 Auto return Function f there are no key operations for 90 seconds the way of setting mode Key Lock mode and Function mode a device automatically returns to chamber temperature display without memorizing value t
81. ons Error code and chamber temperature are displayed alternately Remote alarm contact activates with buzzer sounds Installed on back side Internal overcurrent breaker Safety functions Heater turns off when upper limit temperature alarm is occurred or when temperature sensor is abnormal Valve is closed when 2 sensor is abnormal Fan motor stops rotating when an outer door leaves open Except during CO2 calibration measurement mode 2 valve is closed when an outer door leaves open UV lamp goes off when an outer door leaves open Control panel UV indicator Lights when UV lamp lights DOOR lamp Lights when outer door is open RH PAN lamp Blinks when humidifying water is less than 0 81 when MCO 18UVS3 is optionally installed OVER HEAT lamp Lights when chamber temperature reaches upper limit temperature HEAT lamp Lights when heater is energized INJECT lamp Lights when CO2 gas is injected CO2 gas supply lamp A B Alternative lamp A or B lights when it is currently used either supply line A or B SET Setting key CAL Calibration key BUZZER Press this key to stop buzzer sounding P Digit shift key 4 Numerical value shift key ENT Enter key mPerformance specifications MCO 18AC UV 0 25 C ambient temp 25 C setting 37 C CO 5 no load Temperature variation 0 1 C ambient temp 25 C setting 37 C CO 5 no load Max 2 6A Max 1 4A Max 1 4A 1116 KJ h Temperature 5 35 C Humidity
82. ou assess the work site to ensure there is suitable and sufficient ventilation If restricted ventilation is suspected then other methods of ensuring a safe environment must be considered These may include atmosphere monitoring and warning devices NWARNING Do not use the unit outdoors Current leakage or electric shock may result if the unit is exposed to rain water Only qualified engineers or service personnel should install the unit installation by unqualified personnel may cause electric shock or fire Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from turning over f the floor is not strong enough or the installation site is not adequate this may result in injury from the unit falling or tipping over Never install the unit in a humid place or a place where it is likely to be splashed by water Deterioration of the insulation may result which could cause current leakage or electric shock D Never install the unit in a flammable or volatile location This may cause explosion or fire S Never install the unit where acid or corrosive gases are present as current leakage or electric shock may result due to corrosion Gr ground earth the unit to prevent electric shock If the power supply outlet is not grounded it will be necessary to install a ground by qualified engineers Q Never ground the unit through a gas pipe water main telephone line or lightning rod Such ground
83. pply excessive force to the sensor lead wire 79 30 CALIBRATION Temperature calibration 31 1 Press the calibration key CAL for approximately 5 seconds again to enter the temperature calibration input mode 2 The left digit of the digital temperature indicator blinks and the digital density indicator goes out 3 Set the present correct temperature with the digit shift key bb and numerical value shift key then press the enter key ENT 4 The incubator automatically reverts to the display mode Example If the displayed chamber temperature is 37 0 C set value and the actual temperature is 36 8 C 1 Press the calibration key CAL for about 5 seconds 2 The 3 on the digital temperature indicator blinks and the digital density indicator goes out 3 Adjust the set value to the actual value of 36 8 C with the digit shift key and numerical value shift key then press enter key ENT 4 The incubator automatically reverts to the display mode 80 CALIBRATION Span setting Span setting should be done under stable condition of temperature humidity and density 1 Press the calibration key CAL once to enter the CO calibration pre conditioning mode 2 Confirm blinking the decimal point in the digital density indicator 3 Open the outer door and remove the sample air outlet cap on the inner door 4 Measure the density in the chamber throu
84. r 89 40 Front panel Stacking plate B Stacking plate A Protective sticker Hook Fig A 41 90 SPECIFICATIONS Tempered glass Tray 3 trays made of stainless steel containing copper W450 x D450 x H12 mm Maximum load 7 kg tray Inner diameter 30 mm On the back side 0 3 u m Efficiency 99 97 or more UVlam 1 ozone free emission Water level sensor o Obialpe _ Low humidifying water alarm Automatic set temperature alarm Automatic set density alarm Upper limit temperature alarm various gas sensor heater alarms Allowable contact capacity DC 30 V 2 A Stacking plate and B 2 protective stickers 2 tube bands Optional accessory UV system kit MCO 18UVS3 UV replacement kit MCO 20UV 4 20mA Interface MCO 420MA USA only Gas pressure regulator MCO 100L Extra tray MCO 47ST including 2 tray supports Half tray MCO 25ST Automatic CO cylinder changeover system MCO 21GC Roller base MCO 18RB RS232C RS485 Interface MTR 480 Ethernet LAN Interface MTR LO3 SANYO Data acquisition software MTR 5000 Note Design or specifications will be subject to change without notice 91 42 Ambient temperature 5 C to 50 C ambient temperature 5 C to 35 C 0 25 C ambient temperature 25 C setting 37 C 5 no load 0 1 C ambient temperature 25 C setting 37 C CO 5 no load 0 15 ambient temperature 25 C
85. r 5 seconds to step to Key Lock mode A numerical value will be changed by pressing this key In chamber temperature display press this key for about 5 seconds to step to temperature calibration mode Press this key again to step to CO calibration mode When the is set in 0 a device only steps to temperature calibration mode In temperature calibration mode a device steps to Function mode if you input function code FXX by pressing When chamber temperature is stabilized chamber temperature is within set temperature 3 C press CAL key to enable a device to enter calibration measuring mode In CO calibration measuring mode fan will not stop circulation if an outer door is opened Dot on the CO display will blink during this mode 2 calibration measuring mode will be finished automatically by buzzer sounding 3 times after 5 minutes elapse If CAL key is pressed again during CO calibration measuring mode a device will restart this mode for another 5 minutes Press this key to store a setting value in setting mode calibration mode and Function mode This key is available only when Auto changer mode is set in F08 Auto changer mode is optional function In Auto changer mode press this key to switch a cylinder in use to alternative another one lf one of Aor B lamp illuminates it means that cylinder A or B is in current used When a cylinder in used becomes empty another c
86. r 70 seconds or more Door heater control Basically door heater is turned on by same rate as main heater and it is turned on by the rate which is predetermined by FO7 Remote alarm contact Normal condition Close between COM and N C Open between COM and N O Alarming condition Open between COM and N C Close between COM and N O Ring back time and linkage action between remote alarm and buzzer will be changed depend on the setting in F24 and F25 Prevention for temperature CO overshooting It is removed numerical value 0 2 0 3 from setting value on temperature or CO display during pull up It is not removed a value from setting value on temperature or CO display when a device returns from overshooting with setting value 0 4 more CO display stabilization Buffer rate which is just before CO2 level is displayed is more than setting value 0 05 and less than setting value setting value will be displayed Ex 4 95 Buffer rate 5 00 gt 5 00 will be displayed Ring back Purpose To prevent someone from pressing BUZZER key to stop alarm buzzer and remote alarm activating when a device is in alarming condition Operation 1 Alarm buzzer and remote alarm are active again after predetermined setting time elapses if someone presses BUZZER key Correlation between alarming condition and Ring back Ring back time 30 minutes Not Ring back F25 030 F25 000 Press BUZZER key t
87. r is poured the chamber temperature drops significantly Clean the humidifying once a month e Do not place the incubator in the direct air flow from an air conditioning system Air from an air conditioning system may cause condensation and lead to possible contamination 64 PRECAUTIONS FOR CULTURES e Do not subject to direct air flow Do not allow the air for air conditioning to hit the incubator or door directly Direct hit may cause condensation or contamination e Allow adequate space between the cultures When storing cultures in the chamber keep the Petri dishes or bottles containing the cultures sufficiently apart from each other to allow adequate air circulation Inadequate space may result in uneven temperature distribution and concentration in the chamber e Stored materials Never place acid or alkaline materials or materials that release corrosive gas in the chamber Such materials can cause failure resulting from discoloration or corrosion e Always shut the inner door Shut the inner door completely and then shut the outer door If the inner door is not closed completely even if the outer door is closed the incubator will fail to exhibit its maximum performance And close the doors gently Rude closing may cause spillage of medium incomplete closing or damage of gasket e Open close the doors gently Ensure you close the doors gently Robust closing may cause spillage of medium incomplete closing or da
88. r is supplied SSR is open circuit When SSR for main heater bottom heater and door heater is open circuited E16 and a chamber temperature are displayed alternately and audible alarm sounds intermittently An error code is not always displayed just after failed because the self diagnosis for SSR and heater is done in every 40 minutes or when a power is supplied UV lamp malfunction Note UV lamp is optional component It is necessary to select 001 UV lamp is installed in F20 beforehand If there is malfunction on UV lamp E18 and a chamber temperature are displayed alternately Audible alarm sounds intermittently Press BUZZER key to cancel the error If a door is opened within 30 seconds while a unit detects a voltage the unit will not detect UV because counting is cleared Upper limit temperature alarm When a chamber temperature is over setting temperature for high temperature alarm OVERHEAT lamp illuminates displaying chamber temperature and E12 main heater malfunction or E16 SSR for each heater is open circuited alternately Audible alarm sounds intermittently Audible alarm does not silence by pressing BUZZER key At the time heaters main heater bottom heater and door heater will be OFF Automatic temperature alarm When a chamber temperature deviates from set temperature 1 0 C all digits in temperature display will flash and audible alarm sounds intermittently in 15 minutes later Aut
89. rs use two stacking plates A and B for each one Only one pair of stacking plates is provided per incubator so take out the stacking plates A and B from both incubators Similarly only 2 protective stickers are provided per incubator 2 Unplug both of the incubators that are to be stacked 3 Remove the caps at the two places in the front of the top surface of the incubator that is to be on the bottom and attach the protective stickers to the four comers on the top surface of the incubator that is to be on the bottom See Fig A on the page 41 4 Secure stacking plates A with the screws that are provided 5 Remove the front panel 4 screws from the incubator that is to be on the top 6 Place the upper incubator on top of the lower one being careful not to pinch your fingers 7 Make the lower incubator level by adjusting the leveling feet and then level the upper incubator in the same way 8 Secure stacking plates A on the top surface of lower incubator to the upper one with the accessory SCrews 9 Remove from the back of the lower incubator the two hooks 4 screws and remove from the upper incubator the 2 screws on the lower rear panel 10 Use the 6 screws that were removed in step 9 to secure stacking plates B to the rear panels of the upper and lower incubators 11 Replace the front panel 4 screws to the upper incubator 12 Use the hooks on the back of the upper incubator to prevent the incubators from falling ove
90. s i 1 a Pd E EN 04 nr i AYTAY NOLDM NVA 1 1 IDEYA I i e 5 gt nN E 66 22 oe a A AV13H HOSN3S ZO L s HOSN3S i _ Aver B IET aca HOSNIS HALVA jg H3ONVHO OlnV 24 20 Ur _ El DOY BELHSI iY J39NVHO 1 SA IVA ZN ZO Agee i AV6 A91 JAZZ 6521 PER MR DR Nes Ld gt 5 V H39NVHO O1nv 3A1VA 209 5 20212 9 2 E 3 go e 5 gt for pe ust a dAvi An SEUIL Xe i Cad a Heu i ee d iiM mim m q i ef de 2 22222 Ri j av m 59 9 ON IN i E 408 or 5 EN E m lins H31VA 2 5 misma gt ae EO e TIS oo ENT 09 GN 09 O W ta Nm 17 q 14744 5 58 ranma ONT TENYS RE Tone SAT E i ASE ET 6 01 3 i Ld ZH l Y LL age LES Ti j ZEE H L i 1 LIT EEE b YS0 OlBS to x N gt n NfZozov 7221 o
91. s active or a power failure has occurred The key operation is disable e The key lock function is set in ON mode At the beginning of operation e The chamber temperature is not equal to the set value e The chamber CO density is not equal to the set value a The secondary pressure of the pressure regulator is not equal to the set value 0 03MPaG 0 3kgf cm G 4 3psiG b The tube is not connected securely between the pressure regulator and the incubator During operation upper limit alarm temperature is not set at least 1 C higher than the set chamber temperature e The set temperature value was changed or the door was left open for a long period Or a low temperature load was placed inside the incubator In this case if the incubator is left as it is the alarm will eventually clear itself e The gas tube has slipped off or the gas leaks e The set value of the gas density was changed The gas cylinder is empty Check the primary pressure of the cylinder once a week The primary pressure of less than 3 8MPaG 38kgf cm G means a little gas in the cylinder Replace the cylinder soon ambient temperature must always be at least 5 C less than the set temperature e The outer door was closed while the inner door was left open e The secondary pressure is not set to 0 03MPaG 0 3kgf cm G 4 3psiG e The gas tube is clogged or chinked e The humidifying pan is not filled with sterile distilled water
92. ter that pull the humidifying pan forward See Fig 1 2 Dispose of the remaining water in the humidifying pan and clean the humidifying pan with a neutral dishwashing detergent Then rinse it thoroughly with distilled water and wipe it with sterilizing alcohol 3 Wipe all moisture from the bottom of the chamber 4 Return the humidifying pan to the chamber and add sterile distilled water approx 1 5 L preheated to 37 C See Fig 2 Note Preheat to 37 C the water to be added to the humidifying pan Adding low temperature water will lower the temperature and humidity in the chamber 5 Set the humidifying pan with the inner side flush against the back and close the inner and outer doors MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed Set the humidifying pan with the inner side flush against the back and replace the humidifying cover Refer to page 24 Close the inner and outer doors and confirm that water level alarm lamp RH PAN on the control panel is off Note MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed If the water level alarm lamp RH PAN blinks use the above procedure to replenish the water CAUTION MCO 18AC UV or when an optional UV system kit MCO 18UVS3 is installed When refilling the water in the humidifying pan always wipe off any dirt from the water level sensor with sterilizing alcohol While doing that be careful not to a
93. teria into the chamber e Installation in a sterile room To get the cultivation more efficiently install the incubator in a sterile room e Use clean containers The contamination is mainly caused by the containers such as Petri dishes or bottles stored in the chamber Always keep the containers clean e Always keep the chamber clean The condensation may be caused on the inside of the door by spilled water form humidifying pan or opening of outer door for long period Wipe off the condensation completely with a sterile dry gauze Especially when the culture medium is spilled clean and disinfect the chamber immediately Refer to page 27 Routine maintenance for details e Keep the inside panels dry To protect the inside of the incubator from contamination the inside panels should always be kept dry If water is spilled from a humidifying pan or if the door is kept open for a long period condensation will form on the panels allowing germs to breed In such a case wipe away the water with a dry sterile gauze Particularly if the medium is spilled wipe it up immediately and sterilize the area e Fill the humidifying pan with sterile distilled water Always use sterile distilled water to fill the pan The water level alarm lamp RH PAN on the control panel blinks when the water level is low Refill the sterile distilled water to the pan when the water level alarm lamp RH PAN blinks Note that when low temperature sterile distilled wate
94. the figure to 1 digit changes The key lock is set to ON 4 Press enter key The current chamber temperature is displayed Note e The key lock function is available for temperature and density setting e To cancel the key lock set to LO in the above procedure 23 72 WATER LEVEL SENSOR MCO 18AC UV MCO 18AC with MCO 18UVS3 Water level sensor for the humidifying pan is used with the MCO 18AC UV or the MCO 18AC with an optional UV system kit MCO 18UVS3 installed The sensor is set in active position with respect to the installation of the humidifying pan Take care not to damage the sensor at the time of removal or installation of the humidifying pan When the humidifying pan is removed side view Sensor Humidifying pan When the humidifying pan is installed side view Sensor NC O Note Atthe installation check the humidifying pan is located far depth and the sensor is down Note Lift the sensor before installing the humidifying pan if the sensor is in lower position after maintenance At the time of installation of humidifying pan check that the pan is set properly and sensor is down in the pan The water level alarm lamp RH PAN blinks if the sensor is not down completely this case set the pan again in adequate location The sensor detects the water level every 30 minutes In addition detection is executed after operation of the outer door It takes several seconds to detect t
95. then press the enter key ENT 7 The incubator automatically reverts to the display mode Note It is important to accurately measure the temperature inside the incubator when calibrating the temperature Particularly the temperature gauge used must have an accuracy of JIS 0 5 Class or better The temperature must be measured at several points The temperature setting must not change by more than 1 0 C during calibration If these values are exceeded an error buzzer will sound the input data will be ignored and the incubator will return to the display mode If it is necessary to change the temperature by more than 1 0 C perform calibration in several stages over a period of time 81 32 TROUBLESHOOTING the incubator does not seem to be working check the following items before calling for service Malfunction Check Remedy 33 The incubator does not operate at all If the alarm function and the buzzer operates If the chamber temperature is not equal to the set temperature If the gas density does not coincide with the set value If the chamber humidity does not rise If the consumption is too much If normal cultivation cannot be done and chamber gas density is suspect If it takes much time to recover the gas density gas is not being injected e The incubator is not plugged correctly into a power outlet e The circuit breaker at the power source i
96. tor E06 is displayed alternately with the temperature on the digital temperature indicator E09 is displayed alternately with the temperature on the digital temperature indicator E10 is displayed alternately with the temperature on the digital temperature indicator E11 is displayed alternately with the temperature on the digital temperature indicator E12 is displayed alternately with the temperature on the digital temperature indicator E13 is displayed alternately with the temperature on the digital temperature indicator E14 is displayed alternately with the temperature on the digital temperature indicator E16 is displayed alternately with the temperature on the digital temperature indicator Intermittent tone Intermittent tone Intermittent tone Intermittent tone Intermittent tone Intermittent tone Intermittent tone Intermittent tone Intermittent tone Continuous tone Heater OFF Remote alarm Intermittent tone with Remote alarm with 15 15 minutes delay All digits on the digital Intermittent tone with density indicator blink 15 minutes delay minutes delay minutes dela Fan motor OFF valve is closed UV lamp OFF 60 seconds after Heater OFF The setting mode is canceled The setting is disabled Remote alarm Gas supply line is altered Remote alarm Heater OFF Remote alarm Remote alarm CO valve is closed Remote alarm Low hum
97. tting is 5 0 and an actual measured value is 4 5 Press CAL key Press CAL key for approx 5 seconds The 3 digit on temperature display is flashing and other digits will go off Press CAL key again The 3 digit on CO display is flashing other digits will go off Input 4 5 4 5 by using and 4 key 8 Press ENT key to return the device to chamber temperature display lt Note gt 1 If density is less than 2 0 on CO display an error tone will emit to inform that the input data is invalid Device automatically returns to chamber temperature display Prior to commence calibration ensure to measure CO2 density for 3 times at least to avoid misdiagnosis Ensure capacity of analyzer should be less than 0 2L injection min Ensure to confirm chamber humidity should be more than 90 so that there should be no difference between actual density and CO display Commence CO calibration with 30 minutes delay after a door is open then closed When a chamber temperature deviates from set temperature 3 C CO calibration and measurement mode will be finished automatically Fan will stop activating when an outer door is open If you press CAL key during a unit is in CO calibration measurement mode a unit will start calibration measurement mode for another 5 minutes A fan will activate if an outer door is open CO calibration Note Ensure to
98. ubator is left where outsiders enter it may result unexpected accident for example children to become locked inside Before disposing a CO incubator with biohazardous danger decontaminate the CO incubator to the extent possible by the user 83 34 Note This symbol mark and recycle system are applied only to EU countries and not applied to the countries in the other area of the world Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC English Your SANYO product is designed and manufactured with high quality materials and components which can be recycled and reused This symbol means that electrical and electronic equipment at their end of life should be disposed of separately from your household waste Please dispose of this equipment at your local community waste collection recycling centre In the European Union there are separate collection systems for used electrical and electronic products Please help us to conserve the environment we live in German Ihr SANYO Produkt wurde entworfen und hergestellt mit qualitativ hochwertigen Materialien und Komponenten die recycelt und wiederverwendet werden konnen Dieses Symbol bedeutet da elektrische und elektronische Ger te am Ende ihrer Nutzungsdauer von Hausmull getrennt entsorgt werden sollen Bitte entsorgen Sie dieses Gerat bei Ihrer rtlichen kommunalen Sammelstelle oder im Recycling Centre In der Europaischen Union gibt es
99. unterschiedliche Sammelsysteme f r Elektrik und Elektronikger te Helfen Sie uns bitte die Umwelt zu erhalten in der wir leben 84 Votre produit Sanyo est con u et fabriqu avec des mat riels et des composants de qualit sup rieure qui peuvent tre recycl s et r utilis s Ce symbole signifie que les quipements lectriques et lectroniques en fin de vie doivent tre limin s s par ment des ordures m nag res Nous vous prions donc de confier cet quipement votre centre local de collecte recyclage Dans l Union Europ enne il existe des syst mes s lectifs de collecte pour les produits lectriques et lectroniques usag s Aidez nous conserver l environnement dans lequel nous vivons Les machines ou appareils lectriques et lectroniques contiennent fr quemment des mati res qui si elles sont trait es ou limin es de mani re inappropri e peuvent s av rer potentiellement dangereuses pour la sant humaine et pour l environnement Cependant ces mati res sont n cessaires au bon fonctionnement de votre appareil ou de votre machine Pour cette raison il vous est demand de ne pas vous d barrasser de votre appareil ou machine usag avec vos ordures m nag res Spanish Los productos SANYO est n disefiados y fabricados con materiales y componentes de alta calidad que pueden ser reciclados y reutilizados Este s mbolo significa que el equipo el ctrico y electr nico
100. utomatically Connect the main cylinder to port A and the reserve cylinder to port B After connecting the cylinders check to make sure that no gas is leaking 4 Set the pressure on the secondary side to 0 03 MPaG 0 3 kgf cm G 4 3 psiG at gas injection Excessive pressure may cause disconnection of internal pipes inside the CO incubator which will result in leakage of CO gas into the atmosphere Elevated level of CO gas can be hazardous to health and may lead to asphyxiation and risk of death The repair of the incubator will be necessary if the internal pipe is disconnected 4 Check that no gas is leaking at any point where the pipe connects with the CO regulator or the CO incubator Note The incubator including the gas supply pipes and services must be examined at frequent intervals to ensure they are safe Ensure that items such as pipes are replaced if there is any sign of deterioration 63 14 PREVENTING CONTAMINATION 15 To prevent contamination of the chamber select an appropriate location for installation as well as the complete disinfection of the chamber components e Avoid hot and humid location Avoid location with high temperature and or humidity as the presence of bacteria in the air is greater than normal environment e Avoid drafty location and location with many passers by Avoid locations near doors air conditioners fans etc where slight breezes can facilitate the entry of bac
101. while the UV lamp is lit the lamp will turn OFF Then when the door is closed the lamp will light for the preset period of time e f the UV lamp ON time is set for longer than 5 minutes or if only the outer door is repeated opened and closed condensation may occur in the chamber and may affect temperature distribution It will also shorten the service life of the UV lamp e When replacing the UV lamp contact a Sanyo sales representative or agent Pressing calibration key CAL for about 5 seconds leads the calibration mode In the calibration mode the calibration of temperature and CO density is possible Wrong key operation affects the basic performance Never touch any other keys on the control panel in the event of pressing calibration key CAL accidentally After about 90 seconds the incubator returns to chamber temperature display mode automatically 21 0 Lighting the UV lamp for 24 hours The clauses below are applicable when the model is MCO 18AC UV or an optional UV system kit MCO 18UVS3 is installed If the chamber has been contaminated by dirt or by spilling the medium use the following procedure to decontaminate the chamber by lighting the UV lamp for 24 hours 1 Remove all attachments from the chamber including the trays tray supports side supports fan cover duct fan humidifying pan and humidifying pan cover Clean all the attachments in an autoclave or with dis
102. written permission of Sanyo NCAUTION SANYO guarantees the product under certain warranty conditions SANYO in no way shall be responsible for any loss of content or damage of content 51 2 PRECAUTIONS FOR SAFE OPERATION It is imperative that the user complies with this manual as it contains important safety advice ltems and procedures are described so that you can use this unit correctly and safely lf the precautions advised are followed this will prevent possible injury to the user and any other person Precautions are illustrated in the following way WARNING Failure to observe WARNING signs could result a hazard to personnel possibly resulting in serious injury or death CAUTION Failure to observe CAUTION signs could result in injury to personnel and damage to the unit and associated property Symbol shows N this symbol means caution e this symbol means an action is prohibited this symbol means an instruction must be followed Be sure to keep this manual in a place accessible to users of this unit lt Label on the unit gt This mark is labeled on the cover in which the electrical components of high voltage AN are enclosed to prevent the electric shock The cover should be removed by a qualified engineer or a service personnel only NWARNING with any equipment that uses CO gas there is a likelihood of oxygen depletion in the vicinity of the equipment It is important that y
103. ylinder is used in turn At that time a lamp for cylinder which becomes empty starts flashing and another lamp for cylinder which is used in turn will illuminate 14 Temperature control Setting range 5 Display range 0C 99 9C How to set chamber Press SET key and set a value by pressing n key and pp key temperature Press ENT key to store the value and the device steps to setting mode Unacceptable When a value which deviates from setting range is input and ENT key is setting range pressed buzzer sounds for 1 second and the previous value remains Control PID control Temperature alarm When a chamber temperature deviates from setting temperature 1 C digits will flash and audible alarm sounds and remote alarm outputs after 15 minutes later CO control Setting range 0 20 Display range 0 99 9 How to set Press SET key two times level Set CO2 level by pressing key and gt Press ENT key to store the value and a device steps to Overheat protection temperature check mode Unacceptable When a value which deviates from setting range is input and ENT key is setting range pressed audible alarm sounds for 1 second and the previous value remains Control ON OFF control When CO level is lower than setting value 0 06 control is ON When CO level is higher than setting value 0 01 CO control is OFF Control OFF When setting value is 0 0 display w
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