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ProFlex™ PCR System User Guide (Pub. no. MAN0007697 Rev2.0)

1. 57 System specifications 59 Location of power point and ports on the instrument 60 APPENDIX D Predefined Run Methods 61 APPENDIX E Safety 74 Symbols on this instrument 74 Safety alerts on this instrument 75 Location of safety labels on this instrument 76 Contents 4 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Safety information for instruments not manufactured by Life Technologies Corpora tion 78 Instrument safety 78 General
2. 78 Physical injury 79 Electrical 79 Cleaning and decontamination 79 Laser 79 Safety and electromagnetic compatibility EMC standards 80 Safety 80 EMC 80 Environmental design 80 Chemical safety 81 Biological hazard safety 82 Document
3. 28 Editing a run method 31 Managing methods and folders 37 Performing a run 40 Monitoring a run 41 Monitoring via the Home screen 41 Monitoring via the run monitor screen 42 If the power fails 43 Viewing and exporting the run report 43 Removing the samples from the instrument 44 CHAPTER 4 Routine Maintenance 45 As needed maintenance 45
4. 9 Entering text 10 Entering numerals 11 ProFlex Touchscreen menu overview 12 CHAPTER 2 Initial Set up 14 Site requirements 14 Environmental requirements 14 Temperature and humidity requirements 14 Pollution 14 Altitude 14 Materials 15 Required materials
5. Temperature 68 0 Hold Time 60 AmpliTaq Gold 360 Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 95 0 72 0 Hold Time 300 15 420 Step 2 RampRate 2 3 2 3 Temperature 52 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 Appendix D Predefined Run Methods D 64 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures AmpliTaq Gold Fast PCR Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 96 0 72 0 Hold Time 600 3 10 Step 2 RampRate 2 3 2 3 Temperature 62 0 4 0 Hold Time 3 Infinite Step 3 RampRate 2 3 Temperature 68 0 Hold Time 5 AmpliTaq Gold Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 95 0 72 0 Hold Time 300 15 420 Step 2 RampRate 2 3 2 3 Temperature 55 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Temperature 72 0 Hold Time 30 AmpliTaq Method Cycle Step Stage Reaction volu
6. Temperature 55 0 Hold Time 30 Step 3 RampRate 2 3 Temperature 72 0 Hold Time 60 Platinium Taq High Fidelity Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 30 15 Infinite Step 2 RampRate 2 3 Temperature 55 0 Hold Time 15 Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 Platinium Taq SuperMix Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 30 1 50 105 0 Appendix D Predefined Run Methods D ProFlex PCR System User Guide 71 For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 30 30 Infinite Step 2 RampRate 2 3 Temperature 55 0 Hold Time 15 Step 3 RampRate 2 3 Temperature 72 0 Hold Time 60 SuperScript III 1 Step System Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 40 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 60 0 94 0 68 0 Hold Time 900 15 60 Step 2 RampRate 2 3 2 3 2 3 Temperature 94 0 55 0
7. System Chapter 2 Initial Set up Setting up the ProFlex System 2 ProFlex PCR System User Guide 19 For Research Use Only Not for use in diagnostic procedures Figure 13 ProFlex PCR System Network Connection screen 4 In the Network Connection screen touch Wired The Network Configuration screen opens up Figure 14 ProFlex PCR System Network Configuration screen In the Network Configuration screen you can choose to connect to the network by obtaining the IP address either automatically using DHCP or manually If your instrument is not on a network you do not need to set the IP address Note Ask your system administrator if the IP address is assigned statically or dynamically For static addresses you need to know the IP address for the in strument the subnet mask and the default gateway Automatically Chapter 2 Initial Set up Setting up the ProFlex System 2 20 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Touch Obtain an IP address automatically using DHCP A check mark ap pears when DHCP is selected or Manually Touch Use the following IP Address then enter the appropriate IP address es for the instrument the Subnet Mask and optionally the Default Gate way the Primary DNS Server and the Secondary DNS Server using the nu meric editor Addresses are in the form of X X X X where each X is a 3 digit number from 001 to 25
8. 3 Remove the current sample block from the base 4 Place the sample of block of your choice onto the base 5 Pull the lever towards the left and into the instrument to secure the module Chapter 2 Initial Set up Changing sample blocks 2 24 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Operating the ProFlex PCR System Loading samples into the instrument This section describes how to load samples in Tubes with attached caps 96 well reaction plates 96 well tray retainer assemblies Consumable MicroAmp Optical 96 Well Reaction Plate 0 2mL MicroAmp Reaction Tube with Cap 3 Loading samples in the ProFlex 96 Well PCR System ProFlex PCR System User Guide 25 For Research Use Only Not for use in diagnostic procedures Consumable MicroAmp 8 Tube Strip or MicroAmp Reaction Tube without Cap Loading tubes with attached caps There is no need to use mineral oil or glycerine in the sample wells or as a vapor bar rier over the PCR reaction mixture in the tubes The MicroAmp Reaction Tubes fit tightly in the wells and the heated cover exerts an even pressure on all tubes elimi nating condensation on the tubes 1 Set the 96 well tray on a splash free 96 well base 2 Place the reaction tubes in the tray 3 Pipette the samples into the reaction tubes 4 Cap the tubes 5 See Placing the sample tray
9. 9 Remove the USB drive from the computer when the download is complete To upgrade the ProFlex Instrument with the new firmware 1 Insert the USB drive with the new firmware in the USB port at the rear end of the ProFlex PCR System 2 In the Home screen touch Settings 3 In the Settings screen touch Maintenance amp Services 4 In the Maintenance amp Services screen touch Software Updates The Upgrade Firmware screen opens Chapter 4 Routine Maintenance As needed maintenance 4 48 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 39 ProFlex PCR System Software Updates screen 5 Touch the name of the file with the new firmware then touch Upgrade 6 Touch Yes to confirm that you want to upgrade IMPORTANT Ensure that you do not turn of the instrument when the upgrade is in progress When the upgrade process is complete the instrument will restart automatically Use the Self Verification Test feature for the instrument to check the ProFlex instru ment hardware The check includes testing the block heated cover and other compo nents Note Carry out the Self Verification test periodically or whenever there is an inter mittent instrument error Self Verification test Chapter 4 Routine Maintenance As needed maintenance 4 ProFlex PCR System User Guide 49 For Research Use Only Not for use in diagnostic procedures Figure 40 Pr
10. System 3x32 Well Sample Block Location of safety labels on this instrument Appendix E Safety Safety alerts on this instrument E 76 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 43 Location of the safety label on the ProFlex System96 Well Sample Block Figure 44 Location of the safety label on the ProFlex Dual 96 Well Sample Block Appendix E Safety Safety alerts on this instrument E ProFlex PCR System User Guide 77 For Research Use Only Not for use in diagnostic procedures Figure 45 Location of the safety label on the ProFlex Dual Flat Sample Block Safety information for instruments not manufactured by Life Technologies Corporation Some of the accessories provided as part of the instrument system are not designed or built by Life Technologies Corporation Consult the manufacturer s documentation for the information needed for the safe use of these products Instrument safety CAUTION Do not remove instrument protective covers If you remove the protective instrument panels or disable interlock devices you may be exposed to serious hazards including but not limited to severe electrical shock laser ex posure crushing or chemical exposure General Appendix E Safety Safety information for instruments not manufactured by Life Technologies Cor poration E 78 ProFlex PCR System User Guide For Research Use Only Not for use in diagn
11. 0 25 C 35 to 99 9 C Temperature range for protocol run 0 C to 100 0 C Temperature uniformity For each block lt 0 5 C 30 seconds after reaching 95 C Overall across two blocks lt 0 75 C 30 seconds after reaching 95 C Dimensions Height 27 2 cm 10 7 in Width 33 0 cm 13 in Depth 56 5 cm 22 in Weight 19 3 kg 42 5 lb 19 6 kg 43 2 lb PCR volume range 10 100 L lt 1 L Instrument memory USB on board Display interface 8 4 color TFT LCD Power 100 240 V 50 60 Hz Max 950 W Max Current 12 A Note For Australia AU New Zealand NZ only 230 VAC 50 Hz Max 950 W Max Current 10 A Ambient humidity 15 80 Relative Humidity non condensing acceptable range Ambient operating temperature 15 C to 30 C acceptable range Appendix C Instrument specifications Technical specifications C 58 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Table 12 Power and communication ports Port Description AC power cable port 10 100 Fast Ethernet port for connecting to a network USB v1 0 port for connecting to an external network drive jump drive or other USB storage device RS232 serial communication port for connecting the probe for the Temperature Verification Kit USB v1 0 port for connecting to USB enabled Wi Fi Card System specifications Table 13 Physical dimensions weight and power consumption Parameter I
12. High Specificity AccuPrime PFX 2 Step Method AccuPrime PFX 3 Step Method AccuPrime Taq DNA Polymerase Method AccuPrime Taq High Fidelity Method Next Gen Sequenc ing TargetSeq Exome Enrichment Method Long PCR Elongase 2 Step Method Elongase 3 Step Method AccuPrime Taq High Fidelity Method Platinum Taq High Fidelity Method These run methods cannot be edited or deleted but if you save them with a different name or to a different folder you can edit them The following sections provide the method details AccuPrime PFX 2 Step Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 60 15 Infinite Step 2 RampRate 2 3 Temperature 68 0 Hold Time 60 AccuPrime PFX 3 Step Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 25 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 120 15 Infinite Step 2 RampRate 2 3 Temperature 55 0 Appendix D Predefined Run Methods D 62 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Hold Time
13. 3 Close the heated cover This section describes how to load samples into the MicroAmp reaction tubes or the MicroAmp tube strips into a ProFlex 3X32 Well Sample Block 1 Pipette the samples into the MicroAmp reaction tubes or the MicroAmp tube strips 2 If you are using the MicroAmp tube strips secure the tube strip with the MicroAmp cap strip You can use the MicroAmp Cap Installing Tool Handle to fix and remove the cap or cap strip 3 Place the MicroAmp 3x32 Well Retainer for VeriFlex Systems onto the sample block IMPORTANT The retainer is essential and Life Technologies recommends its us age at all times along with the ProFlex 3x32 Well PCR System Loading samples in the ProFlex 3x32 Well PCR System Chapter 3 Operating the ProFlex PCR System Loading samples into the instrument 3 ProFlex PCR System User Guide 27 For Research Use Only Not for use in diagnostic procedures Figure 16 MicroAmp 3x32 Well Retainer Part number 4481669 4 Place the MicroAmp reaction tubes or the MicroAmp tube strips within the re tainer that s been placed on the sample block 5 Close the heated cover Creating a new run method 1 In the Home screen touch New Method Figure 17 ProFlex 3x32 Well System Touchscreen Home The Setup Run screen opens up 2 In the Setup Run screen touch Open Template Open Method or Incubate Chapter 3 Operating the ProFlex PCR System Crea
14. 7 For Research Use Only Not for use in diagnostic procedures Figure 1 ProFlex 3x32 Well PCR System Figure 2 ProFlex 96 Well PCR System This user guide provides information on how to maximize the capabilities of your ProFlex PCR System This user guide provides unique instructions for the different ProFlex block types at the beginning of each section Detailed specifications for the ProFlex PCR System are found in Appendix C In strument specifications Chapter 1 About the ProFlex PCR System About the system 1 8 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Using the ProFlex System Touchscreen You interact with the ProFlex System using a touchscreen Table 1 describes the sev eral buttons common to many of the screens in the ProFlex System Table 1 Buttons in the ProFlex System touchscreen Button Function Returns to the previous screen 1 Current temperature 2 Time remaining displays time elapsed if the block is in an infinite incubation stage 3 Status of the block pre heating run ning and so on 4 Block number in case of the ProFlex 3x32 Well Sample Block Scrolls up and down through a list of items and buttons View other stages Closes the current modal window Indicates whether the Wi Fi is on or off Chapter 1 About the ProFlex PCR System Using the ProFlex System Touchscreen
15. PCR System Using the ProFlex System Touchscreen 1 ProFlex PCR System User Guide 11 For Research Use Only Not for use in diagnostic procedures ProFlex Touchscreen menu overview On powering on the ProFlex PCR System you will first see the following Splash screen Figure 3 ProFlex PCR System Splash screen After the Splash screen the Home screen appears The ProFlex PCR System has in terchangeable blocks and detects which module is present on the system automatical ly After the block completes initializing depending on the block in use the Home screen displays status dials three for the ProFlex 3x32 Well Sample Block and one for the ProFlex 1x96 Well Sample Block the ProFlex Dual 96 Well Sample Block and the ProFlex Dual Flat Sample Block Chapter 1 About the ProFlex PCR System ProFlex Touchscreen menu overview 1 12 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 4 ProFlex 3x32 Well PCR System Home screen The Home screen includes the following features 1 Status dial The number of dials appearing on the Home screen varies with the block module in use While the ProFlex System is in use the status dial displays the current temperature of the block the time elapsed for a run and the run sta tus When the ProFlex System is not in use the status dial s will show the Set Up Run display You can start a run by touc
16. 100 0 C Temperature uniformity lt 0 5 C 30 seconds after reaching 95 C Dimensions Height 27 2 cm 10 7 in Width 33 0 cm 13 in Depth 56 5 cm 22 in Weight 18 75 kg 41 lb PCR volume range 10 80 L Instrument memory USB on board Display interface 8 4 color TFT LCD Power 100 240 V 50 60 Hz Max 950 W Max Current 12 A Note For Australia AU New Zealand NZ only 230 VAC 50 Hz Max 950 W Max Current 10 A VeriFlex blocks 3 x 32 well 0 2 mL independent control 5 C 2 VeriFlex zones 96 well 0 2 mL VeriFlex block 25 C 5 C zone to zone 6 VeriFlex zones C ProFlex PCR System User Guide 57 For Research Use Only Not for use in diagnostic procedures Block format Specification Ambient humidity 15 80 Relative Humidity non condensing acceptable range Ambient operating temperature 15 C to 30 C acceptable range The following table displays the system specifications for the ProFlex PCR System using the ProFlex Dual 96 Well Sample Block and the ProFlex Dual Flat Sample Block Table 11 ProFlex PCR System technical specifications ProFlex Dual 96 Well Sample Block and the ProFlex Dual Flat Sample Block Block format Specification ProFlex Dual 96 Well Sample Block ProFlex Dual Flat Sample Block Maximum block ramp rate 3 00 C sec 1 60 C sec Maximum sample ramp rate 1 60 C sec N A Temperature accuracy
17. 15 Optional protective hardware 15 Unpacking the ProFlex System 15 Setting up the ProFlex System 16 How to set up the wired connection in the ProFlex System 19 How to set up the wireless connection in the ProFlex System 21 Recommended instrument settings 22 Changing sample blocks 23 CHAPTER 3 Operating the ProFlex PCR System 25 Loading samples into the instrument 25 Loading samples in the ProFlex 96 Well PCR System 25 ProFlex PCR System User Guide 3 For Research Use Only Not for use in diagnostic procedures Loading samples in the ProFlex 3x32 Well PCR System 27 Creating a new run method
18. 4 0 Hold Time 120 30 Infinite Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 TargetSeq Exome Enrichment Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 72 50 105 0 Step 1 RampRate 2 3 Temperature 47 0 Appendix D Predefined Run Methods D 72 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Hold Time 3600 Time Release Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 40 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 95 0 72 0 Hold Time 60 15 420 Step 2 RampRate 2 3 2 3 Temperature 55 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Temperature 72 0 Hold Time 30 Touchdown PCR Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 20 10 50 105 0 Step 1 RampRate 2 3 2 3 Temperature 94 0 94 0 Hold Time 15 15 Step 2 RampRate 2 3 2 3 Temperature 65 0 55 0 Hold Time 30 30 AutoDelta Temperature 0 5 AutoDelta Starting Cy cle 1 Appendix D Predefined Run Methods D ProFlex PCR System User Guide 73 For Research Use Only Not for use in diagnos
19. Predefined Run Methods D ProFlex PCR System User Guide 69 For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Temperature 55 0 Hold Time 15 Platinium PFX 2 Step Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 120 15 Infinite Step 2 RampRate 2 3 Temperature 68 0 Hold Time 60 Step 3 RampRate Temperature Hold Time Platinium PFX 3 Step Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 120 15 Infinite Step 2 RampRate 2 3 Temperature 55 0 Hold Time 30 Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 Appendix D Predefined Run Methods D 70 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Platinium Taq DNA Polymerase Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 30 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 30 30 Infinite Step 2 RampRate 2 3
20. for indoor use Ensure that the installation site Meets the spatial and weight requirements see System specifications on page 59 Meets environmental requirements see Environmental requirements on page 14 Is within 1 m 3 ft of an AC power source receptacle Is away from water Environmental requirements Ensure that the installation site is maintained under the following conditions Table 4 Temperature and humidity requirements Condition Acceptable range Temperature 15 to 30 C 59 to 86 F Humidity 15 to 80 relative humidity non condens ing Avoid placing the instrument adjacent to heaters cooling ducts or in direct sunlight Fluctuations between day and night temperatures can cause system instability Place away from any equipment that vibrates such as a refrigerator or centrifuge The ProFlex PCR System has a Pollution Degree rating of 2 It may be installed in an environment that has non conductive pollutants only such as dust particles or wood chips Typical environments with a Pollution Degree II rating are laboratories and sales and commercial areas The safety of ProFlex PCR System use was tested for altitudes up to 6000 ft 2 Temperature and humidity requirements Pollution Altitude 14 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Materials Scissors pocket knife or box cutter Compre
21. onto the base Chapter 2 Initial Set up Setting up the ProFlex System 2 16 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 7 ProFlex System Base unit with the latching mechanism to the left Note If the sample block module is not seated in place correctly the instrument will not be able to detect the block type Note To change the sample block from one type to the other see Changing sample blocks on page 23 3 Close the heated cover 4 Connect the power cable to the ProFlex System Figure 8 Power cable port in the ProFlex System Base unit 5 Optional Install any of the recommended protective devices see Optional pro tective hardware on page 15 6 Connect the power cable to the AC power source receptacle 7 Connect the instrument to the network by inserting the ethernet cable into the ethernet port at the back of the ProFlex System For instructions on setting up the wired connection see How to set up the wired connection in the ProFlex System on page 19 In the absence of the ethernet cable or the ethernet port you can connect the in strument to the network via the USB enabled Wi Fi Card Part no 4483658 as shown in the following graphic Insert the Wi Fi Card into the USB port at the back of ProFlex System For in structions on setting up the wireless connection see How to set up the wireless connection in the ProFl
22. the chemical manufacturer Obtaining Certificates of Analysis The Certificate of Analysis provides detailed quality control and product qualification information for each product Certificates of Analysis are available on our website Go to www lifetechnologies com support and search for the Certificate of Analysis by product lot number which is printed on the box Obtaining support For the latest services and support information for all locations go to www lifetechnologies com support ProFlex PCR System User Guide 83 For Research Use Only Not for use in diagnostic procedures At the website you can Access worldwide telephone and fax numbers to contact Technical Support and Sales facilities Search through frequently asked questions FAQs Submit a question directly to Technical Support techsupport lifetech com Search for user documents SDSs vector maps and sequences application notes formulations handbooks certificates of analysis citations and other product support documents Obtain information about customer training Download software updates and patches Limited product warranty Life Technologies Corporation and or its affiliate s warrant their products as set forth in the Life Technologies General Terms and Conditions of Sale found on Life Tech nologies website at www lifetechnologies com termsandconditions If you have any questions please contact Life Technologie
23. the power failed Note If the time between the initial power failure and the time the power returns is more than 12 hours the run is not continued Viewing and exporting the run report To view a report 1 In the Home screen touch Settings 2 In the Settings screen touch Run History 3 In the Run History screen touch the Run ID of the run you want to view The report is displayed If the power fails Chapter 3 Operating the ProFlex PCR System Viewing and exporting the run report 3 ProFlex PCR System User Guide 43 For Research Use Only Not for use in diagnostic procedures 4 Touch Export to save the report on a USB drive Removing the samples from the instrument Grasp the sample tray retainer assembly or reaction plate firmly with both hands and gently rock it back and forth until the tubes or plate are free CAUTION Sample caps may pop off if the cover is opened when the sample block s temperature is above 27 C Chapter 3 Operating the ProFlex PCR System Removing the samples from the instrument 3 44 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Routine Maintenance As needed maintenance Preparation Before cleaning the instrument 1 Power off the ProFlex Instrument by disconnecting the power 2 Allow the instrument to cool until the heated cover and sample block s reach room temperature Cleaning the touchscreen Clean t
24. the status of a run using the Run Monitoring screen as shown in the following figure Figure 38 ProFlex System Run Monitoring screen The screen shows the run method profile and additional information that does not ap pear in the Dial on the Home screen Monitoring via the run monitor screen Chapter 3 Operating the ProFlex PCR System Monitoring a run 3 42 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Table 5 Elements of the Run Monitor screen Element Description Start Time Displays the time a run started Time Remaining Displays the time remaining for a run End Time Displays the approximate time at which the run will end Provides more details about the run Touching Details brings up the Progress Details screen with the following run infor mation Run ID Simulation Mode Start time Estimated End time Sample Temperature Heated Cover Reaction Volume Helps skip the current holding step How ever if the run is currently ramping the Skip button will not function Helps pause a run Touching Pause changes the button to Touch to continue the run Helps stop a run After a run has completed or been aborted the Home screen appears again If a power failure occurs during a run the instrument attempts to continue the run when the power returns The run begins at the first step of the stage where
25. type of the network Note When you touch in the Network Name field a keypad will come up to facilitate making the entry 5 From the Security type drop down menu touch to select the security type and enter the relevant information in the screen Note Contact your IT Systems Administrator for information on security type You can select from the following options WEP WPA Personal WPA2 Personal WPA Enterprise WPA2 Enterprise 6 Touch the Remember this network checkbox if you want the ProFlex System to remember the network Touch Join to continue or Cancel to exit from the Find and Join a Network screen 7 Depending on the security type you have selected enter the appropriate pass words and touch Join If you entered the correct information the Network Connection Complete screen will appear Touch OK to continue If you entered incorrect information the Net work Connection Failed screen will come up and you will require to go back to security type screen to resolve the issue Touch OK to continue You can configure the following instrument settings by touching Settings on the Home screen Device Settings Touch Device Settings to set various additional parameters Set the following in the Device Settings screen Instrument Name Touch the Instrument Name field and using the text edi tor enter up to 25 alphanumeric or 10 numeric characters to identify the in strument N
26. 1 ProFlex PCR System User Guide 9 For Research Use Only Not for use in diagnostic procedures When you touch a field that requires the input of text the text editor as seen in the following figure opens Table 2 displays the buttons to touch to enter different infor mation types Table 2 Buttons to enter different information To Touch Enter a lowercase letter A letter button Enter an uppercase letter then the letter s When you are done touch again Enter punctuation or other symbols then the symbol s When you are done touch again Delete the last letter Close the editor and save any changes to accept the entry and close the editor Close the editor and discard any changes Entering text Chapter 1 About the ProFlex PCR System Using the ProFlex System Touchscreen 1 10 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures When you touch a field that requires a numerical input the numeric editor as seen in the following figure opens Table 3 displays the buttons to touch to perform different functions while entering numbers Table 3 Functions performed by entering numbers To Touch Enter a number A number button Enter decimal colon or infin ity symbol the button with the respective symbol Delete the last number Close the editor and save any changes Entering numerals Chapter 1 About the ProFlex
27. 10 Infinite Step 2 Ramp Rate 2 3 Tempera ture 50 0 Hold Time 5 Step 3 Ramp Rate 2 3 Tempera ture 60 0 Hold Time 75 BigDye Direct PCR Run Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 96 0 72 0 Hold Time 600 3 120 Step 2 RampRate 2 3 2 3 Temperature 62 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Temperature 68 0 Hold Time 30 BigDye Kit Fast Run Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 25 1 50 105 0 Appendix D Predefined Run Methods D ProFlex PCR System User Guide 67 For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Step 1 RampRate 2 3 2 3 2 3 Temperature 96 0 96 0 4 0 Hold Time 60 10 Infinite Step 2 RampRate 2 3 Temperature 50 0 Hold Time 5 Step 3 RampRate 2 3 Temperature 60 0 Hold Time 75 BigDye Kit Standard Run Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 25 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 96 0 96 0 4 0 Hold Time 60 10 Infini
28. 15 Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 AccuPrime Taq DNA Polymerase Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 25 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 120 15 Infinite Step 2 RampRate 2 3 Temperature 55 0 Hold Time 15 Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 AccuPrime Taq High Fidelity Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 25 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 15 15 Infinite Step 2 RampRate 2 3 Temperature 52 0 Hold Time 15 Step 3 RampRate 2 3 Appendix D Predefined Run Methods D ProFlex PCR System User Guide 63 For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Temperature 68 0 Hold Time 60 AmpliTaq 360 Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 25 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 15 15 Infinite Step 2 RampRate 2 3 Temperature 52 0 Hold Time 15 Step 3 RampRate 2 3
29. 379983 MicroAmp 96 Well Reaction Tray Retainer for VeriFlex Systems 4381850 MicroAmp 3x32 Well Retainer for VeriFlex Sys tems 4481669 Seals and Covers MicroAmp Clear Adhesive Film 0 2 96 Well 4306311 MicroAmp Optical Adhesive Film 4360954 4311971 MicroAmp Optical Adhesive Film Kit 4313663 96 Well Plates MicroAmp Optical 96 Well Reaction Plate 0 2 96 Well N8010560 4316813 MicroAmp Optical 96 Well Reaction Plate with Bar code 4306737 4326659 MicroAmp Optical 96 Well Reaction Plate with Bar code and Optical Caps 403012 MicroAmp Optical 96 Well Reaction Plate with Bar code and Optical Adhesive Films 403012 8 Well Strips 2 Appendix A Ordering Information Consumables A 52 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Consumable Model For mat Cat No MicroAmp 8 Tube Strip 0 2 mL 0 2 32 Well and 96 Well N8010580 MicroAmp 8 Tube Strip Assorted Colors 0 2 mL N8010838 MicroAmp Optical 8 Tube Strip 0 2 mL 4316567 MicroAmp 8 Cap Strip N8011535 MicroAmp 8 Cap Strip Assorted Colors N8010835 MicroAmp Optical 8 Cap Strip 4323032 1 There are no 32 well Microplates available for use with the ProFlex 3x32 Well PCR System Instead single tubes with caps are used for running the PCR samples 2 There are no 32 well Microplates available for use with the ProFlex 3x32 Well PCR
30. 5 5 Touch OK to save the changes and go back to the Network Connection screen Touch Cancel to exit the screen without saving the changes See Setting up the ProFlex System on page 16 on connecting the USB enabled Wi Fi Card into the ProFlex System To set up the wireless connection 1 Refer to How to set up the wired connection in the ProFlex System on page 19 through step 3 to arrive at the Network Connection screen 2 In the Network Connection Type screen touch Wireless Figure 15 ProFlex PCR System Network Connection screen The Search Wireless Network screen will come up and the wireless symbol will appear active Touch Cancel to exit the screen Note During initial setup if you selected the Wired option in the Network Con nection Type screen you will be required to enter your IP address if you selected the Static IP wired option If you selected the Dynamic IP wired option the IP address is automatically populated 3 Once a wireless connection has been detected a list of the available networks will be displayed in the Choose Network screen Touch the network name of your choice or touch Join Other Network How to set up the wireless connection in the ProFlex System Chapter 2 Initial Set up Setting up the ProFlex System 2 ProFlex PCR System User Guide 21 For Research Use Only Not for use in diagnostic procedures 4 In the Find and Join a Network screen enter the name and security
31. CR System touch the dial corresponding to the block that you are using to perform the run Reaction volumes are specific to the type of instrument ProFlex 1x96 Well PCR System 10 to 80 L ProFlex 3x32 Well PCR System 10 to 80 L 4 Touch Run ID then enter the applicable information The default Run ID is YYYYMMDDHHMM where YYYYMMDD is the date and HHMM is the current time You can choose to retain the default Run ID 5 Touch Start Run The cover is heated to the required temperature then the run begins While the run is in progress the Home screen is displayed When the run is com plete the Status Dial s on the Home screen displays Done and Remove Samples 6 Touch Done once you have removed the samples The Status Dial is now ready for another run and displays Set Up Run Monitoring a run After a run has started the Home screen appears again with the dials updated with current information on the run The dials display the following run information Current block temperature Time remaining since the run started Run status Monitoring via the Home screen Chapter 3 Operating the ProFlex PCR System Monitoring a run 3 ProFlex PCR System User Guide 41 For Research Use Only Not for use in diagnostic procedures To monitor the run progress touch the dial that is running to go the Run Monitoring screen Figure 37 ProFlex System Home screen You can follow
32. Cleaning the instrument 45 Replacing fuses 47 Upgrading system firmware 47 Self Verification test 49 Ordering kits and replacement parts 50 APPENDIX A Ordering Information 51 Instrument part numbers 51 Consumables 51 APPENDIX B Troubleshooting 54 Troubleshooting problems 54 Returning an instrument for service 55 APPENDIX C Instrument specifications 57 Technical specifications
33. EC European Union Battery Directive MII Order 39 PRC Management Methods for Controlling Pollution by Electronic Information Prod ucts Chemical safety WARNING GENERAL CHEMICAL HANDLING To minimize hazards en sure laboratory personnel read and practice the general safety guidelines for chemical usage storage and waste provided below and consult the relevant SDS for specific precautions and instructions Read and understand the Safety Data Sheets SDSs provided by the chemi cal manufacturer before you store handle or work with any chemicals or hazardous materials To obtain SDSs see the Documentation and Support section in this document Minimize contact with chemicals Wear appropriate personal protective equipment when handling chemicals for example safety glasses gloves or protective clothing Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended in the SDS Handle chemical wastes in a fume hood Ensure use of primary and secondary waste containers A primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste material and meet federal state and local requireme
34. Is network functioning Try a different Ethernet cable Cycling time too long Peltier assembly failure Run the Cycle Performance Test When running on multiple instru ments cannot see instruments in Se lect Instruments screen Instrument s not connected to net work Switch powered off Ethernet cable bad IP address conflict Allow Remote Runs setting in Instru ment Name screen set to No Are all Ethernet cables connected to the instruments Are all Ethernet cables connected to the network switch Try a different Ethernet cable Change the IP addresses on the in struments In Instrument Name screen change Allow Remote Runs to Yes B 54 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Problem Possible cause Check and or remedy No screen display No response when you power on the instrument Blown fuse Not connected to power source Check fuses Is power switch on Is power cord connected Screen display is missing characters or is illegible LCD screen failure Request service Sample tubes melt during run Incompatible consuma bles used Heated cover too hot Sample block s too hot Use recommended consumables see Appendix A Ordering Information Run the Heated Cover Verification Test Heating cover stuck Cam platen or cover assembly malfunc
35. System Instead tube strips with caps or cap strips are used for running the PCR samples Table 9 ProFlex PCR System sample handling supplies Part Name Cat No MicroAmp Adhesive Film Applicator 4333183 MicroAmp Multi Removal Tool 4313950 MicroAmp Cap Installing Tool Handle 4330015 MicroAmp 96 Well Base N8010531 MicroAmp Splash Free 96 Well Base 4312063 MicroAmp Centrifuge Adapter N8013822 Appendix A Ordering Information Consumables A ProFlex PCR System User Guide 53 For Research Use Only Not for use in diagnostic procedures Troubleshooting Troubleshooting problems Use the following table to help troubleshoot problems with your ProFlex System Problem Possible cause Check and or remedy Cooling rate too slow Peltier assembly failure Run the Cycle Performance Test Temperature does not match specified temperature Instrument may require calibration Run the Temperature Verification Test Heating rate too slow Peltier failure Run the Cycle Performance Test Printing fails Printer incorrectly configured on in strument Printer not connec ted to network Instrument not connected to net work Ethernet cable bad Make sure the printer IP address on the instrument matches the IP ad dress from the system administra tor Is Ethernet cable connected to the printer Is Ethernet cable connected to the instrument
36. USER GUIDE For Research Use Only Not for use in diagnostic procedures ProFlex PCR System User Guide Installation Use and Maintenance for use with PCR reagents from Invitrogen and Applied Biosystems Catalog Number 4483636 4483637 4483638 4484071 4484073 4484074 4484075 4484076 and 4484078 Publication Number MAN0007697 Revision 2 0 For Research Use Only Not for use in diagnostic procedures The information in this guide is subject to change without notice DISCLAIMER LIFE TECHNOLOGIES CORPORATION AND OR ITS AFFILIATE S DISCLAIM ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT EXPRESSED OR IMPLIED INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE OR NON INFRINGEMENT TO THE EXTENT ALLOWED BY LAW IN NO EVENT SHALL LIFE TECHNOLOGIES AND OR ITS AFFILIATE S BE LIABLE WHETHER IN CONTRACT TORT WARRANTY OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL INCIDENTAL INDIRECT PUNITIVE MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT INCLUDING BUT NOT LIMITED TO THE USE THEREOF LIMITED USE LABEL LICENSE Research Use Only Notice to Purchaser The purchase of this product conveys to the purchaser the limited non transferable right to use the purchased amount of the product only to perform internal research for the sole benefit of the purchaser No right to resell this product or any of its components is conveyed expressly by implic
37. a b In the Edit AutoDelta screen enter the information for setting up Auto Delta Enter the Starting Cycle Enter the DELTA temperature and or time Use the and but tons set the DELTA values to be either positive or negative respec tively Touch Remove AutoDelta to remove the AutoDelta setting Touch Done when finished or Cancel to exit the screen Figure 30 Edit AutoDelta c Touch Done to return to the Edit Method screen Chapter 3 Operating the ProFlex PCR System Editing a run method 3 36 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Once you have edited a run method you can save the method to the destination folder by touching Save in the Run Method preview screen or choose to start a run without saving the run method 4 Touch Save to save the edited run method Figure 31 ProFlex System Run Method preview screen a In the Save screen enter a name of the edited run method Note Names can be up to 100 characters Names may not contain any of the following characters lt gt amp tab Spaces under scores and dashes are allowed b Touch a folder for example to select a destination folder where you want to save the run method 5 Touch Save when you are done Managing methods and folders Use this feature to copy a run method from one folder to another rename a particular method name or delete
38. a method 1 In the ProFlex System Home screen touch Open Method Touching Open Method takes you to the Select Method Screen Chapter 3 Operating the ProFlex PCR System Managing methods and folders 3 ProFlex PCR System User Guide 37 For Research Use Only Not for use in diagnostic procedures Figure 32 ProFlex System Home screen 2 In the Select Method screen touch Manage Folders Figure 33 ProFlex System Select Method screen Chapter 3 Operating the ProFlex PCR System Managing methods and folders 3 38 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 34 ProFlex System Manage Folders screen 3 In the Manage Folders screen Touch Copy to copy a run method from one existing folder to another For example you can copy a run method from the Public folder into the USB folder You can also copy a run method from an existing folder into a new folder Note Back up your method files to a USB drive frequently or before send ing in your ProFlex System for service To create a new folder 1 Select a run method s that you want to copy by touching the one or more run methods name under Method Name then touch Copy 2 In the Copy Method screen select the folder you would like to copy the methods to or touch New Folder Chapter 3 Operating the ProFlex PCR System Managing methods and folders 3 ProFlex PCR System User Guide 39 For Researc
39. acing fuses Upgrading system firmware Chapter 4 Routine Maintenance As needed maintenance 4 ProFlex PCR System User Guide 47 For Research Use Only Not for use in diagnostic procedures You will need a USB drive and if your instrument requires login Administrator ac cess to upgrade the firmware IMPORTANT You cannot upgrade the firmware while a run is in progress Upgrading the ProFlex PCR System firmware 1 Open the About Instrument screen to determine the version number of the exist ing firmware To open the About Instrument screen in the Home screen touch Settings In the Settings screen touch About Instrument 2 Record the version number of the firmware then touch Done 3 On a computer connected to the Internet go to www lifetechnologies com to de termine the version number of the new firmware 4 Click Support then Software Downloads 5 Select ProFlex PCR System in the list then click Updates amp Patches 6 Find the appropriate file If the version number is The same as the current version from step 3 you do not need to upgrade the firmware Different from the current version from step 3 follow the steps below to download and upgrade the firmware 7 Insert a USB drive into the USB port on the computer to download the new firm ware to the USB drive 8 Click the link in the Software column to download the file Select the USB drive as the location for the saved file
40. atego ry These run methods are based on recommended protocols from Life Technologies and should be considered as starting points for the different applications Your results may vary between instrument models so some optimization may be needed Please note that the ramp rates of these methods are set at 100 The following table lists the categories and the run methods they include Table 14 Pre defined categories and run methods Category Run method Blank Template General PCR Run Method Basic PCR AmpliTaq 360 Method AmpliTaq Method Basic PCR Taq Method Hot Start AmpliTaq 360 Method AmpliTaq Gold Method AmpliTaq Gold Fast PCR Run Method Platinum Taq DNA Polymerase Method Platinum Taq SuperMix Method Sequencing BigDye Direct Cycle Sequence Method BigDye Direct PCR Run Method BigDye Kit Fast Run Method BigDye Kit Standard Run Method Optimization Time Release Run Method Touchdown PCR Run Method RT PCR SuperScript III 1 Step System Method High Fidelity AccuPrime PFX 2 Step Method AccuPrime PFX 3 Step Method AccuPrime Taq High Fidelity Method Platinum PFX 2 Step Method Platinum PFX 3 Step Method Platinum Taq High Fidelity Method D ProFlex PCR System User Guide 61 For Research Use Only Not for use in diagnostic procedures Category Run method
41. ation or by estoppel This product is for internal research purposes only and is not for use in commercial applications of any kind including without limitation quality control and commercial services such as reporting the results of purchaser s activities for a fee or other form of consideration For information on obtaining additional rights please contact outlicensing lifetech com Trademarks The trademarks mentioned herein are the property of Life Technologies Corporation and or its affiliate s or their respective owners AmpliTaq and AmpliTaq Gold are registered trademarks of Roche Molecular Systems Inc Clorox is a registered trademark of The Clorox Company 2013 Life Technologies Corporation All rights reserved Contents About this Guide 6 Revision History 6 Purpose 6 CHAPTER 1 About the ProFlex PCR System 7 About the system 7 Using the ProFlex System Touchscreen
42. ation and Support 83 Related documentation 83 Obtaining SDSs 83 Obtaining Certificates of Analysis 83 Obtaining support 83 Limited product warranty 84 Contents ProFlex PCR System User Guide 5 For Research Use Only Not for use in diagnostic procedures About this Guide Revision History Revi sion Date Description 01 May 2013 New document 02 August 2013 Added information about the ProFlex Dual 96 Well PCR System and the ProFlex Dual Flat PCR System Purpose The ProFlex PCR System User Guide provides information about installing using and maintaining the ProFlex PCR System 6 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures About the ProFlex PCR System The ProFlex PCR System is an end point thermal cycler specifically designed for the ampl
43. ature 2 Edit the idling temperature if the button is in the On mode Note The temperature for the heated cover must be between 30 and 110 C 3 Click OK to save the changes or Cancel to exit the screen Remote Service In the Remote Service screen use the On and Off toggle button to enable or disable respectively remote service The feature allows the instrument to upload data periodically to a remote server Note Ensure that you are connected to a network if you want to enable the Remote Service feature After you have set the toggle button touch OK to save the change or Cancel to exit the screen About Instrument Touching About Instrument will take you to the About In strument screen where you can find out more information about the instrument as well as view the End User License Agreement EULA Touch About Instrument to find out more about the ProFlex PCR System In the About Instrument screen you can view information like firmware ver sion and instrument statistics Touch EULA to view the End User License Agreement You can also save the EULA document to a USB drive Run History The Run History screen displays the entire list of runs performed using a particular ProFlex PCR System a In the Run History screen touch a particular Run ID to view the details of that run b Touch Export to save the run details to a USB device Maintenance amp Services You can perform the foll
44. d labora tory use Part 2 010 Particular require ments for laboratory equipment for the heating of materials 21 CFR 1040 10 and 1040 11 as applicable U S FDA Health and Human Services HHS Radiological health performance stand ards for laser products and Radiological health performance standards for specific purpose laser products Reference Description Directive 2004 108 EC European Union EMC Directive EN 61326 1 Electrical Equipment for Measurement Control and Laboratory Use EMC Re quirements Part 1 General Requirements FCC Part 18 47 CFR U S Standard Industrial Scientific and Medical Equipment AS NZS 2064 Limits and Methods of Measurement of Electromagnetic Disturbance Characteris tics of Industrial Scientific and Medical ISM Radiofrequency Equipment ICES 001 Issue 3 Industrial Scientific and Medical ISM Ra dio Frequency Generators Reference Description Directive 2012 19 EU European Union WEEE Directive Waste electrical and electronic equipment Safety EMC Environmental design Appendix E Safety Safety and electromagnetic compatibility EMC standards E 80 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Reference Description Directive 2011 65 EU European Union RoHS Directive Re striction of hazardous substances in elec trical and electronic equipment Directive 2006 66
45. ell PCR System 4484073 ProFlex Dual 96 Well PCR System 4484076 ProFlex Dual Flat PCR System 4484078 Table 7 ProFlex Sample Blocks Description Cat No ProFlex 96 Well Sample Block 4483637 ProFlex 3x32 Well Sample Block 4483638 ProFlex Dual 96 Well Sample Block 4484071 ProFlex Dual Flat Sample Block 4484074 Consumables IMPORTANT In Table 8 the Model Format column indicates the model s of the ProFlex System that are compatible with the associated kit or disposable Before or dering verify that your ProFlex System supports the kit or disposable Table 8 ProFlex PCR System Consumables Consumable Model For mat Cat No Single Tubes 1 A ProFlex PCR System User Guide 51 For Research Use Only Not for use in diagnostic procedures Consumable Model For mat Cat No MicroAmp Reaction Tube with Cap 0 2 mL 32 Well and 96 Well N8010540 N8011540 MicroAmp Reaction Tube with Cap Assorted Colors 0 2 mL N8010840 MicroAmp Reaction Tube with Cap Autoclaved 0 2 mL N8010612 MicroAmp Reaction Tube without Cap 0 2 mL N8010533 N8011533 MicroAmp Reaction Tube without Cap Assorted Colors 0 2 mL N8010833 MicroAmp Optical Tube without Cap 0 2 mL N8010933 MicroAmp 96 Well Reaction Tube Tray Retainer Set 0 2 mL 96 Well 403083 403086 Reaction Trays MicroAmp 96 Well Reaction Tray for VeriFlex Sys tems 0 2 96 Well 4
46. ex System on page 21 Chapter 2 Initial Set up Setting up the ProFlex System 2 ProFlex PCR System User Guide 17 For Research Use Only Not for use in diagnostic procedures Figure 9 USB port in the ProFlex System Base unit 8 Press the power button then wait for the instrument to start up Proceed with the installation after the touchscreen displays the Main Menu indicating that the ProFlex System startup is complete When you power on the instrument the in strument may require about 45 seconds to start up Note See Recommended instrument settings on page 22 for instructions on completing the installation process of the ProFlex System from the Instrument touchscreen The Home screen will display following successful installation Figure 10 ProFlex 3x32 Well System Touchscreen Home Chapter 2 Initial Set up Setting up the ProFlex System 2 18 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures See Setting up the ProFlex System on page 16 for instructions on connecting the ProFlex System to the network To set up the wired connection 1 On the Home screen touch Settings Figure 11 ProFlex PCR System Settings screen 2 In the Settings screen touch Device Settings Figure 12 ProFlex PCR System Device Settings screen 3 In the Device Settings screen touch Network Connection How to set up the wired connection in the ProFlex
47. h Use Only Not for use in diagnostic procedures Figure 35 ProFlex System Copy Method screen 3 If you touched New Folder you will be prompted to edit the name of the new folder in the alphanumeric editor Touch Enter after editing the name 4 Touch Paste Method Touch Rename to rename a particular method folder name Touch Delete to delete a particular method folder Touch Done to save the changes and return to the Select Method screen Performing a run Prepare your samples and load the sample block s as described in Loading samples into the instrument on page 25 1 Close the cover 2 Start a run from the Home screen in one of the following ways Touch New Method Open Method Incubate Status dials Note See Creating a new run method on page 28 to create a new run method or see Editing a run method on page 31 to edit an existing run method 3 Based on the block type you are using touch Choose Block for the ProFlex 3x32 Well Sample Block or Start Run for the ProFlex 96 Well Sample Block The Run Parameters screen is displayed Chapter 3 Operating the ProFlex PCR System Performing a run 3 40 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 36 ProFlex System Run Parameters screen Verify the name of the run method that you have selected and in case of the ProFlex 3x32 Well P
48. having the instrument serviced at your facility or sending the instrument for repair maintenance trade in disposal or termination of a loan decontamination forms may be requested from customer service Before using any cleaning or decontamination methods except those recom mended by the manufacturer users should confirm with the manufacturer that the proposed method will not damage the equipment CAUTION LASER HAZARD Bar Code Scanner The bar code scanner inclu ded with the instrument system is a Class 2 laser To avoid damage to eyes do not stare directly into the beam or point into another person s eyes Physical injury Electrical Cleaning and decontamination Laser Appendix E Safety Instrument safety E ProFlex PCR System User Guide 79 For Research Use Only Not for use in diagnostic procedures Safety and electromagnetic compatibility EMC standards The instrument design and manufacture complies with the standards and require ments for safety and electromagnetic compatibility as noted in the following table Reference Description EU Directive 2006 95 EC European Union Low Voltage Directive IEC 61010 1 EN 61010 1 UL 61010 1 CSA C22 2 No 61010 1 Safety requirements for electrical equip ment for measurement control and labo ratory use Part 1 General requirements IEC 61010 2 010 EN 61010 2 010 Safety requirements for electrical equip ment for measurement control an
49. he touchscreen with any commercially available LCD cleaning product Be careful not to scratch the screen Cleaning the sample wells If you use any cleaning or decontamination method except those recommended in the manual you risk damaging the equipment Clean the sample wells once a month or as needed WARNING Always wear protective glasses and gloves when servicing the in strument Also make sure you disconnect the instrument from AC line power before you begin any service procedure Always wear protective glasses and gloves when servicing the instrument Also make sure you disconnect the in strument from AC line power before you begin any service procedure CAUTION During instrument operation the temperature of the heated cover can be as high as 113 C and the temperature of the sample block s can be as high as 100 C Before performing the procedure keep hands away until the heated cover and sample block s reach room temperature To clean the sample wells 1 Follow the steps in Preparation on page 45 2 Open the heated cover 3 Remove the sample tray from the sample block s and set it aside 4 Use a cotton swab soaked in isopropanol to clean the sample wells thoroughly Make certain that the isopropanol has evaporated completely before reloading a sample tray 4 Cleaning the instrument ProFlex PCR System User Guide 45 For Research Use Only Not for use in diagnostic procedures Decontaminating
50. hing the status dial where it says Set Up Run For instructions on starting a run by this method see Performing a run on page 40 2 My Account Touch My Account to enter or edit the account information 3 New Method Touch New Method to create a new run method with the help of the default templates that are pre loaded with the ProFlex System Touching New Method takes you to the Setup Run screen that allows you to create a new run method by using the default templates or existing methods You can also cre ate a run method for incubation from the Setup Run screen 4 Open Method Touch Open Method to select an existing run method to start a run Touching Open Method takes you to the Select Method screen that displays the existing run methods The run methods are sorted and stored in various fold ers Select a folder to display the run methods within the folder Touch a run method under the Method name to edit that run method in the edit mode 5 Incubate Touch Incubate to use the ProFlex instrument as a precise incubator for non PCR workflows 6 Settings Touch Settings to configure the ProFlex Instrument Chapter 1 About the ProFlex PCR System ProFlex Touchscreen menu overview 1 ProFlex PCR System User Guide 13 For Research Use Only Not for use in diagnostic procedures Initial Set up This chapter includes the procedures for installing the ProFlex PCR System Site requirements The ProFlex PCR System is
51. ification of nucleic acids using the Polymerase Chain Reaction PCR process The user interface consists of a touchscreen with a graphical display that shows the time status and temperature profile for each run A touchscreen keypad allows you to enter information into fields on the display screen About the system There are four models of the ProFlex PCR System Description Cat No ProFlex 96 Well PCR System 4484075 ProFlex 3x32 Well PCR System 4484073 ProFlex Dual 96 Well PCR System 4484076 ProFlex Dual Flat PCR System 4484078 Four interchangeable ProFlex Sample Blocks are available Description Cat No ProFlex 96 Well Sample Block 4483637 ProFlex 3x32 Well Sample Block 4483638 ProFlex Dual 96 Well Sample Block 4484071 ProFlex Dual Flat Sample Block 4484074 The ProFlex PCR System allows you to Run three experiments at a time with the 3x32 well 0 2 mL block Interchange the blocks e g exchange the ProFlex 3x32 Well Sample Block with the ProFlex 96 Well Sample Block the ProFlex Dual 96 Well Sample Block the ProFlex Dual Flat Sample Block and vice versa Access the system remotely through a mobile application Program the instrument from the touchscreen interface Simulate other PCR instruments with Thermal Simulation modes Optimize your PCR with the help of the Veriflex blocks 1 ProFlex PCR System User Guide
52. me L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Appendix D Predefined Run Methods D ProFlex PCR System User Guide 65 For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 95 0 72 0 Hold Time 60 15 420 Step 2 RampRate 2 3 2 3 Temperature 55 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Temperature 72 0 Hold Time 30 Basic PCR Taq Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 95 0 72 0 Hold Time 60 15 420 Step 2 RampRate 2 3 2 3 Temperature 55 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Temperature 72 0 Hold Time 30 BigDye Direct Cycle Sequence Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 5 Cycles 1 1 1 25 1 50 105 0 Step 1 Ramp Rate 2 3 2 3 2 3 2 3 2 3 Appendix D Predefined Run Methods D 66 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 5 Tempera ture 37 0 80 0 96 0 96 0 4 0 Hold Time 900 120 10
53. nces n gatives sur l environnement la suite de l limination de d chets lectroniques ne pas limin er ce d chet lectronique avec les d chets usuels non soumis au tri s lectif Se con former aux ordonnances lo cales sur les d chets munici paux pour les dispositions d limination et communi quer avec le service la cli ent le pour des renseigne ments sur les options d limi nation responsable Safety alerts on this instrument Additional text may be used with one of the symbols described above when more specific information is needed to avoid exposure to a hazard See the following table for safety alerts found on the instrument Appendix E Safety Safety alerts on this instrument E ProFlex PCR System User Guide 75 For Research Use Only Not for use in diagnostic procedures English French translation CAUTION Hazardous chemicals Read the Safety Data Sheets SDSs before handling ATTENTION Produits chimiques danger eux Lire les fiches signal tiques FS avant de manipuler les produits CAUTION Hazardous waste Refer to SDS s and local regulations for handling and disposal ATTENTION D chets dangereux Lire les fiches signal tiques FS et la r glementa tion locale associ es la manipulation et l limination des d chets Figure 41 Location of the safety label on the rear panel of the ProFlex System Figure 42 Location of the safety label on the ProFlex
54. ng biological samples in laboratory environment U S Department of Health and Human Services Biosafety in Microbiological and Biomedical Laboratories BMBL 5th Edition HHS Publication No CDC 21 1112 Revised December 2009 found at www cdc gov biosafety publications bmbl5 BMBL pdf World Health Organization Laboratory Biosafety Manual 3rd Edition WHO CDS CSR LYO 2004 11 found at www who int csr resources publications biosafety Biosafety7 pdf Appendix E Safety Biological hazard safety E 82 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Documentation and Support Related documentation The following related documents are shipped with the system Document Publication num ber Description ProFlex PCR System In stallation and Operation Quick Reference 4483807 Describes the procedures for instal ling and using the ProFlex PCR Sys tem that is described in detail in the ProFlex PCR System User Guide ProFlex PCR System Sys tem Documentation 4483803 Provides the reference for accessing user documentation from the product website and contact details for service and technical support Note For additional documentation see Obtaining support on page 83 Obtaining SDSs Safety Data Sheets SDSs are available from www lifetechnologies com support Note For the SDSs of chemicals not distributed by Life Technologies Corporation contact
55. nstrument foot print Recommended clear ance Total Height 27 2 cm 10 7 in 15 5 cm 6 1 in 1 42 5 cm 16 7 in Width 33 0 cm 13 in 0 cm 0 in 33 0 cm 13 in Depth 56 5 cm 22 in 23 0 cm 9 in 2 79 5 cm 31 in Weight ProFlex PCR System with the ProFlex 96 Well Sample Block or the ProFlex 3x32 Well Sam ple Block 18 75 kg 41 lb ProFlex Dual 96 Well Sample Block 19 3 kg 42 5 lb ProFlex Dual Flat Sample Block 19 6 kg 43 2 lb Power consump tion 950 W Maximum consumption 300 W Average during thermal cycling 10 W During sleep standby 1 With the heated cover open 2 At the rear of the instrument to ensure adequate airflow and cooling Appendix C Instrument specifications System specifications C ProFlex PCR System User Guide 59 For Research Use Only Not for use in diagnostic procedures Location of power point and ports on the instrument The following graphic displays the location of the power cord insertion point as well as the various ports provided in the ProFlex instrument Appendix C Instrument specifications Location of power point and ports on the instrument C 60 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Predefined Run Methods The following run methods distributed across ten categories are supplied with the ProFlex PCR System A particular run method can appear in more than one c
56. nts for container storage After emptying a waste container seal it with the cap provided Characterize by analysis if necessary the waste generated by the particular applications reagents and substrates used in your laboratory Ensure that the waste is stored transferred transported and disposed of ac cording to all local state provincial and or national regulations IMPORTANT Radioactive or biohazardous materials may require special handling and disposal limitations may apply Appendix E Safety Chemical safety E ProFlex PCR System User Guide 81 For Research Use Only Not for use in diagnostic procedures Biological hazard safety WARNING BIOHAZARD Biological samples such as tissues body fluids in fectious agents and blood of humans and other animals have the potential to transmit infectious diseases All work should be conducted in properly equip ped facilities using the appropriate safety equipment for example physical containment devices Safety equipment also may include items for personal protection such as gloves coats gowns shoe covers boots respirators face shields safety glasses or goggles Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state provincial and or national regulations The following references provide gener al guidelines when handli
57. o modify the advanced parameters 3 Touch Advanced Options to edit the VeriFlex AutoDelta Ramp Rates and Simulation Mode parameters Figure 24 Advanced Options screen Touch Simulation Mode to set the simulation mode Note Simulation modes make ProFlex System perform like another instru ment This is done my simulating the ramp rate of another instrument Chapter 3 Operating the ProFlex PCR System Editing a run method 3 32 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 25 Advanced Options Simulation Mode In the Simulation Mode screen a Select the instrument you would like to simulate Touch No Simulation to run the ProFlex instrument in the default mode b Touch Done to save the selection or Cancel to exit the screen c Use the button in the top right corner of the screen to return to the Ad vanced Options screen Touch Ramp Rates to edit the ramp rate of the individual steps Chapter 3 Operating the ProFlex PCR System Editing a run method 3 ProFlex PCR System User Guide 33 For Research Use Only Not for use in diagnostic procedures Figure 26 Advanced Options Ramp Rates a Touch a particular ramp rate to open the editor to edit the ramp rate b Touch Done to save the selection or Cancel to exit the screen c Use the button in the top right corner of the screen to return to the Ad vanced Options screen Note If yo
58. oFlex PCR System Self Verification Test screen Procedure To carry out the Self Verification test 1 In the Home screen touch Settings 2 In the Settings screen touch Maintenance amp Settings 3 In the Maintenance amp Settings screen touch Self Verification Test 4 In the Self Verification Test screen touch Start Test to begin testing or Cancel to exit the screen The test takes about 10 minutes Once the test is completed the test results will be displayed in the form of a report Note For the ProFlex 3x32 Well PCR System all the blocks are tested at the same time Ensure that no run is on at the time of the Self Verification test Note Contact your Life Technologies service representative in case of block fail ure 5 Touch Export to save the test report to a USB drive or Close to exit the screen Ordering kits and replacement parts Refer to Appendix A Consumables on page 51 to obtain part numbers for kits and replacement parts Chapter 4 Routine Maintenance Ordering kits and replacement parts 4 50 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Ordering Information Instrument part numbers Contact your regional Life Technologies sales office to order kits accessories consum ables and spare parts for the ProFlex System Table 6 ProFlex PCR Systems Description Cat No ProFlex 96 Well PCR System 4484075 ProFlex 3x32 W
59. ognized 12 5 A Type Time Lag 5 x 20 mm 250 VAC Note For Australia and New Zealand only Two 10 A Type Time Lag 5 x 20 mm 250 VAC Fine flat tip screwdriver Procedure 1 Power off the ProFlex instrument by disconnecting the power DANGER ELECTRICAL SHOCK HAZARD Severe electrical shock which could cause physical injury or death can result from working on an instrument when the high voltage power supply is operating To avoid electrical shock disconnect the power supply to the instrument unplug the power cord and wait at least 1 minute before working on the instru ment The fuses are in the fuse holder F1 F2 2 Insert a small flat tip screwdriver into the slot in the upper portion of the power entry module and open the door 3 Pull out the fuse compartment to inspect the two fuses in the fuse compartment 4 Pull out the blown fuse s from the fuse compartment and replace with new two UL Recognized 12 5 A Type Time Lag 5 x 20 mm 250 VAC Note For Australia and New Zealand use new two 10 A Type Time Lag 5 x 20 mm 250 VAC 5 Place the fuse compartment back into the power entry module there is only one orientation and close the door 6 Press the door until it locks in place 7 Connect the instrument power cord About the upgrade If Life Technologies provides a new version of the firmware for the ProFlex PCR Sys tem you may be required to load the new firmware on the instrument Repl
60. or plate onto the sample block s on page 27 Loading the reaction plate 1 Place the reaction plate on a splash free 96 well base 2 Pipette the samples into the sample wells 3 Cap the plates using the MicroAmp 8 Cap Strips or cover the plates with MicroAmp Clear Adhesive Film 4 See Placing the sample tray or plate onto the sample block s on page 27 Loading tubes without caps To load the 96 well tray retainer 1 Place the tray on the splash free 96 well base 2 Load tubes onto the tray using either single tubes or the 8 tube strips 3 Place the retainer over the tubes 4 Pipette the sample into the tubes Chapter 3 Operating the ProFlex PCR System Loading samples into the instrument 3 26 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures 5 Cap the tubes using a MicroAmp 8 Cap Strip 6 See Placing the sample tray or plate onto the sample block s on page 27 Placing the sample tray or plate onto the sample block s The steps for placing the sample tray in the sample block s are the same for A sample tray retainer A sample tray without a retainer A 96 well plate 1 Lift the sample tray from the splash free 96 well base and place it in the sample block s 2 Place the MicroAmp Tray or Plate onto the sample block s so that well A1 is at the upper left corner of the tray as shown in the following figure
61. ostic procedures CAUTION Moving Parts Moving parts can crush pinch and cut Keep hands clear of moving parts while operating the instrument Disconnect power before servicing WARNING Sharp Edges The edges of this container are sharp WARNING Ensure appropriate electrical supply For safe operation of the in strument Plug the system into a properly grounded receptacle with adequate current capacity Ensure the electrical supply is of suitable voltage Never operate the instrument with the ground disconnected Grounding con tinuity is required for safe operation of the instrument WARNING Power Supply Line Cords Use properly configured and approved line cords for the power supply in your facility WARNING Disconnecting Power To fully disconnect power either detach or unplug the power cord positioning the instrument such that the power cord is accessible CAUTION Cleaning and Decontamination Use only the cleaning and decon tamination methods specified in the manufacturer s user documentation It is the responsibility of the operator or other responsible person to ensure the fol lowing requirements are met No decontamination or cleaning agents are used that could cause a HAZ ARD as a result of a reaction with parts of the equipment or with material contained in the equipment The instrument is properly decontaminated a if hazardous material is spil led onto or into the equipment and or b prior to
62. ote The instrument name cannot have spaces Separate consecutive char acters with a hyphen or underscore for example My_Instrument Date Time Touch Date Time to set the date and time for a run 1 Enter the date and time in the Date Time field using the numerical edi tor that comes up when you place the cursor in the respective fields You can toggle between AM and PM by touching in the field directly 2 Touch OK to save the date and time changes or Cancel to exit Sleep Mode In the Sleep Mode screen touch the Off and On toggle button to disable or enable respectively the sleep mode In the On mode you can edit the time in minutes after which the instru ment will go from the idling state into standby mode Network Connection Touch Network Connection to select the type of net work connection You can select from the Wireless and Ethernet options For Recommended instrument settings Chapter 2 Initial Set up Setting up the ProFlex System 2 22 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures details on using the Wireless and Ethernet options see How to set up the wired connection in the ProFlex System on page 19 and How to set up the wireless connection in the ProFlex System on page 21 respectively Heated Cover 1 In the Heated Cover screen touch the On and Off button to enable or disable respectively the heated cover idling temper
63. owing in the Maintenance amp Services screen Software Updates Touch Software Updates to update the ProFlex System firmware See Upgrading system firmware on page 47 for instructions on updating the firmware Self Verification Test Touch Self Verification Test for the ProFlex instru ment to conduct a check on the instrument hardware The check includes testing the block heated cover and other components See Chapter 4 Rou tine Maintenance for instructions on conducting the self verification test Changing sample blocks IMPORTANT Ensure that you don t change the block while the ProFlex Instrument is powered on This can cause extreme damage to the hardware itself Chapter 2 Initial Set up Changing sample blocks 2 ProFlex PCR System User Guide 23 For Research Use Only Not for use in diagnostic procedures The ProFlex System features an interchangeable sample block module allowing port ability and versatility in sample configuration The ProFlex System supports the following sample blocks ProFlex 96 Well Sample Block ProFlex 3x32 Well Sample Block ProFlex Dual 96 Well Sample Block ProFlex Dual Flat Sample Block To change the sample block 1 Power down the instrument using the main power switch on the back of the in strument 2 Pull the lever out from the sample block module and move it towards the right to loosen the block from the base
64. pine in a neutral position Bend at the knees and lift with your legs Do not lift and twist your torso at the same time Required materials Optional protective hardware Chapter 2 Initial Set up Materials 2 ProFlex PCR System User Guide 15 For Research Use Only Not for use in diagnostic procedures 3 Verify that the package containing the ProFlex System Accessories includes Power cord cable ProFlex System Starter Kit USB enabled Wi Fi Card Part no 4483658 Setting up the ProFlex System 1 Remove the packing material a Open the ProFlex System heated cover b Remove the packing material from the ProFlex sample block s ProFlex 96 Well Sample Block or ProFlex 3x32 Well Sample Block c Using a can of compressed air deliver a blast of air into each well of the sample block s to remove any particles that may have collected during transportation 2 Install the sample block To place the sample block into the instrument base a Facing the ProFlex Base unit away from you pull the lever all the way to the right hand side Figure 5 ProFlex System Base unit and the ProFlex 3x32 Well Sample Block b Place the sample block onto the ProFlex Base with the latching mechanism to the right Figure 6 ProFlex System Base unit with the latching mechanism to the right c Push the latching mechanism all the way to the left to secure the block
65. r use in diagnostic procedures Editing a run method The Edit Method screen serves as a preview of the run method you have selected If you do not want to edit any of the parameters you can start a run directly by touch ing Choose Block for the ProFlex 3x32 Well PCR System or Start Run for the ProFlex 96 Well PCR System 1 In the Edit Method screen for the run method that you have selected touch Edit or anywhere on the graphical temperature profile to start editing the parameters Figure 22 Options available in the Run Method preview screen You will see that each of the numbers are surrounded by black fields indicating that they are editable At this point you can edit any of the temperatures times heated cover number of cycles and other parameters seen on the screen 2 Touch Manage Steps to manage the steps for example add or delete a stage or step or set more advanced parameters a Touch Add Steps to add a step b Touch Remove Steps to delete a step c Touch Add Stages to add a stage d Touch Remove Stages to delete a stage The buttons appear while adding a step or stage while the buttons appear while deleting a step or stage Chapter 3 Operating the ProFlex PCR System Editing a run method 3 ProFlex PCR System User Guide 31 For Research Use Only Not for use in diagnostic procedures Figure 23 Manage Steps screen Touch Done to finish managing the steps or Advanced Options t
66. rocedure keep hands away until the heated cover and sample block s reach room temperature To clean the heated cover 1 Follow the steps in Preparation on page 45 2 Open the heated cover 3 Soak a cotton swab or piece of clean cloth with isopropanol and gently wipe the heated platen WARNING CHEMICAL HAZARD Isopropanol is a flammable liquid and vapor Exposure may cause eye skin and upper respiratory tract irri tation Prolonged or repeated contact may dry skin and cause irritation Exposure may cause central nervous system effects such as drowsiness dizziness and headache Read the MSDS and follow the handling instruc tions 4 Remove any remaining isopropanol from the cover Note If the platen becomes contaminated with amplified DNA then raise the heated cover to the cleaning position wipe the platen with a cloth or cotton swab soaked in bleach then rinse with water Clean the heated platen once a month or as needed Chapter 4 Routine Maintenance As needed maintenance 4 46 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Decontaminate the heated cover If the heated cover become contaminated with amplified DNA raise the heated cover to the cleaning position and wipe the cover with a cloth or cotton swab soaked in 1 10 v v dilution of 5 25 sodium hypochlorite Clorox bleach then wipe the cover with a damp cloth Required materials Two UL Rec
67. ry Symbol English Fran ais Caution risk of danger Consult the manual for further safety information Attention risque de danger Consulter le manuel pour d autres renseignements de s curit Caution risk of electrical shock Attention risque de choc lectrique Moving parts Parties mobiles Caution sharp edges Attention bords coupants E 74 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Symbol English Fran ais Caution hot surface Attention surface chaude Potential biohazard Danger biologique potentiel Potential slipping hazard Danger de glisser potentiel Standby En attente Protective conductor terminal main ground Borne de conducteur de protection mise la terre principale Terminal that can receive or supply alternating or direct current or volt age Borne pouvant recevoir ou envoyer une tension ou un courant continu ou alternatif Do not dispose of this product in un sorted municipal waste CAUTION To minimize neg ative environmental impact from disposal of electronic waste do not dispose of elec tronic waste in unsorted mu nicipal waste Follow local municipal waste ordinances for proper disposal provision and contact customer service for information about respon sible disposal options Ne pas liminer ce produit avec les d chets usuels non soumis au tri s lectif CAUTION Pour minimiser les cons que
68. s at www lifetechnologies com support Documentation and Support Limited product warranty 84 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Headquarters 5791 Van Allen Way Carlsbad CA 92008 USA Phone 1 760 603 7200 Toll Free in USA 800 955 6288 For support visit lifetechnologies com support or email techsupport lifetech com lifetechnologies com 27 September 2013
69. ser Guide 55 For Research Use Only Not for use in diagnostic procedures 5 Pack the instrument in the provided packaging without any accessories or pow er cords Include a hard copy of the Certificate of Instrument Decontamination in the box Note Repairs for instruments without the Certificate of Instrument Decontami nation are delayed 6 Affix the provided postage to the box then ship the instrument to the designated facility The repair process requires 2 to 3 weeks Appendix B Troubleshooting Returning an instrument for service B 56 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Instrument specifications This chapter lists the instrument specifications for the ProFlex PCR System using the ProFlex 96 Well Sample Block the ProFlex 3x32 Well Sample Block the ProFlex Dual 96 Well Sample Block and the ProFlex Dual Flat Sample Block Technical specifications The following table displays the system specifications for the ProFlex PCR System using the ProFlex 96 Well Sample Block and the ProFlex 3x32 Well Sample Block Table 10 ProFlex PCR System technical specifications ProFlex 96 Well Sample Block and the ProFlex 3x32 Well Sample Block Block format Specification Maximum block ramp rate 6 00 C sec Maximum sample ramp rate 4 40 C sec Temperature accuracy 0 25 C 35 to 99 9 C Temperature range for protocol run 0 C to
70. ssed air Protective hardware as appropriate see below Life Technologies supports the use of the following devices to protect the ProFlex PCR System from damage resulting from electrical hazards and the resultant loss of data Before installing the system decide what additional hardware if any you want to install Power line regulator 1 5 kVA Surge protector line conditioner 10 kVA Uninterruptible power supply 1 5 kVA Unpacking the ProFlex System IMPORTANT Save the packing materials and box in case you need to ship the instru ment to Life Technologies for service 1 To unpack the ProFlex System a Cut the straps securing the instrument box b Cut the tape securing the top flaps of the instrument crate then open the flaps c Remove the ProFlex System Accessories from the instrument and set them aside d Lift and remove the cover from the instrument crate e Remove the packing material from the ProFlex System then inspect the in strument for shipping damage IMPORTANT If the ProFlex System is damaged note the location and ap pearance of the damage then contact Life Technologies Technical Support or your service representative see Obtaining support on page 83 2 Move the ProFlex System to the desired installation site Follow these guide lines for lifting and moving Make sure that you have a secure comfortable grip Keep your s
71. te Step 2 RampRate 2 3 Temperature 50 0 Hold Time 5 Step 3 RampRate 2 3 Temperature 60 0 Hold Time 240 Elongase 2 Step Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 30 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Appendix D Predefined Run Methods D 68 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Hold Time 60 30 Infinite Step 2 RampRate 2 3 Temperature 68 0 Hold Time 60 Elongase 3 Step Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 30 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 94 0 94 0 4 0 Hold Time 30 30 Infinite Step 2 RampRate 2 3 Temperature 55 0 Hold Time 30 Step 3 RampRate 2 3 Temperature 68 0 Hold Time 60 General PCR Method Cycle Step Stage Reaction volume L Cover temp C 1 2 3 4 Cycles 1 35 1 50 105 0 Step 1 RampRate 2 3 2 3 2 3 Temperature 95 0 95 0 72 0 Hold Time 60 15 420 Step 2 RampRate 2 3 2 3 Temperature 55 0 4 0 Hold Time 15 Infinite Step 3 RampRate 2 3 Appendix D
72. the sample wells If the sample wells become contaminated with any biological agents clean the wells thoroughly with a cotton swab soaked in 1 10 v v dilution of 5 25 sodium hypo chlorite Clorox bleach IMPORTANT Use bleach solution in moderation Life Technologies recommends the use of 10 bleach solution for removing contamination from the Veriti instru ment sample block s excessive use of the solution however can corrode the sample block s material To prevent damage to the sample block s Avoid applying excessive amounts of bleach solution If possible instead of using a squeeze bottle or soaked cloth use an atomizer to deliver the solution to the wells of the block s After treating with bleach solution rinse the sample block s thoroughly using de ionized water Note Removing residual bleach from the surfaces of the ProFlex instrument using water minimizes the long term effects of bleach treatments If the sample block s become contaminated with radioactivity use a commercially available decontaminant to remove the contamination If the block s cannot be de contaminated the instrument cannot be returned to Life Technologies for service Cleaning the heated cover Clean the heated cover once a month or as needed CAUTION During instrument operation the temperature of the heated cover can be as high as 113 C and the temperature of the sample block s can be as high as 100 C Before performing the p
73. tic procedures Safety WARNING GENERAL SAFETY Using this product in a manner not specified in the user documentation may result in personal injury or damage to the in strument or device Ensure that anyone using this product has received instruc tions in general safety practices for laboratories and the safety information pro vided in this document Before using an instrument or device read and understand the safety infor mation provided in the user documentation provided by the manufacturer of the instrument or device Before handling chemicals read and understand all applicable Safety Data Sheets SDSs and use appropriate personal protective equipment gloves gowns eye protection etc To obtain SDSs see the Documentation and Support section in this document Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information In this document the hazard symbol is used along with one of the following user attention words CAUTION Indicates a potentially hazardous situation that if not avoided may result in minor or moderate injury It may also be used to alert against un safe practices WARNING Indicates a potentially hazardous situation that if not avoided could result in death or serious injury DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious inju
74. ting a new run method 3 28 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 18 ProFlex System Setup Run screen a Touch Open Template to create a new method using an existing template Select from the categories listed in the Select Method Template screen Figure 19 ProFlex System Select Method Template screen b Touch Open Method to create a new method using an existing run method Select from the methods listed in the Select Method screen Note The Select Method screen opens up if you touch Open Method in the Home screen or in the Setup Run screen Chapter 3 Operating the ProFlex PCR System Creating a new run method 3 ProFlex PCR System User Guide 29 For Research Use Only Not for use in diagnostic procedures Figure 20 ProFlex System Select Method screen 3 Irrespective of the source for creating a new run method once you select one of the methods you will come to the Method Edit screen Figure 21 Preview of Run Method See Editing a run method on page 31 for instructions on editing a run meth od Note You can choose to directly start a run without editing a run method To start a run touch Choose Block for the ProFlex 3x32 Well PCR System or Start Run for the ProFlex 96 Well PCR System Chapter 3 Operating the ProFlex PCR System Creating a new run method 3 30 ProFlex PCR System User Guide For Research Use Only Not fo
75. tion ing Request service Heated cover not responsive Heated cover failure Run the Heated Cover Verification Test Sample tubes are crushed or de formed after run Incompatible consuma bles used or proper trays retainer accesso ries not used Use recommended consumables see Appendix A Ordering Information Returning an instrument for service Before returning an instrument for service back up the run methods and instrument settings as described in Managing methods and folders on page 37 This practice ensures that if the service center sends you a replacement instrument you can restore the run methods and other settings To return an instrument for service 1 Contact your local customer care center or technical support group to obtain a copy of the Certificate of Instrument Decontamination a service notification a service call number and if required packaging 2 Decontaminate the instrument IMPORTANT If the sample block s become contaminated with radioactivity use a commercially available decontaminant to remove the contamination If the block s cannot be decontaminated the instrument cannot be returned to Life Technologies for service 3 Complete and sign a copy of the Certificate of Instrument Decontamination 4 Fax the Certificate of Instrument Decontamination to the customer care center Appendix B Troubleshooting Returning an instrument for service B ProFlex PCR System U
76. u set a simulation mode other than No Simulation then the ramp rate will be locked and unavailable for editing Note Editable ramp rates are shown in the sample ramp rate This sample rate is a more accurate depiction of the block s thermal performance however it is slower than the maximum block ramp rates shown in the specifications section of this user guide Touch VeriFlex to edit the VeriFlex parameter a Touch the icon to choose the steps you want to apply VeriFlex Chapter 3 Operating the ProFlex PCR System Editing a run method 3 34 ProFlex PCR System User Guide For Research Use Only Not for use in diagnostic procedures Figure 27 Advanced Options VeriFlex b In the Edit VeriFlex screen adjust the temperature range across the block for the selected steps Figure 28 Edit VeriFlex Touch Remove VeriFlex to remove the VeriFlex setting Touch Done when finished or Cancel to exit the screen c Touch Done to return to the Edit Method screen d Use the button in the top right corner of the screen to return to the Ad vanced Options screen Touch AutoDelta to apply the AutoDelta setting to a step a Touch the icon to choose the steps you want to apply AutoDelta Chapter 3 Operating the ProFlex PCR System Editing a run method 3 ProFlex PCR System User Guide 35 For Research Use Only Not for use in diagnostic procedures Figure 29 Advanced Options AutoDelt

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ProFlex&trade; PCR System User Guide (Pub. no. MAN0007697 Rev2.0)

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ProFlex™ PCR System User Guide (Pub. no. MAN0007697 Rev2.0)