BIS910 User Guide
Contents
1. While the BIS910 is provided with the Dual UV configuration see details on page 15 below it has only UV illumination sources In order to provide the user with the ability to detect samples which are stained with visible dyes the user should use the visible dye converter as required for converting and detecting samples under UV illumination To Detect Visible Stained Gels Pull out the BIS910 UV drawer Place the Visible Dye Converter on the UV drawer Place your sample on the Visible Dye Converter Turn the illumination on from one of the light sources vv BE UU N e Continue to work as you would without the white light converter Hardware System Overview Fig 12 Visible Dye Converter Dual UV Configuration The standard D Transilluminator is equipped with single type of UV and a single type of white illumination source Upon user request we can implement a second type of UV source e g We can supply two of the following options 254 nm 312 nm 365 nm Implementing these two kinds of UV sources is defined as Dual UV configuration When you use the Dual UV configuration the D Transilluminator lacks white light sources Therefore you will have to use the White Light converter in order to detect samples which are stained with visible dyes Refer to the Visible Dyes Converter immediately above this section The BIS910 will be provided only with the UV drawer and the Visible Dye converter beside of the White Light drawer2. bot Mem ee 177 H n In some cases the resulted image provides only part of the expected bands In order to reveal more bands if any you may use the Automatic stretching button It will automatically stretch the image signals by a software algorithm and will reveal some low intensity bands You can also use the Contrast Low High level sliders as a manual software stretching action If you would like to return to the original image parameters after stretching you should click on the Restore Original button User should be aware that if you make stretching changes to the image and then save them those Note changes are saved with the image when opened in GelCapture If however you open the same stretched image in any other software apart from GelCapture the image appears as it did when originally captured Operating Instructions The image statistics is presented above the image to the left File Image 1tf Date taken 26 6 2014 11 54 8 Exposure 1 45 sec owa lt ae ZZ EEE ae E Pami 8 ml UE 1 ES 25 TREE cT win KE PA A 488820 zadnie LITLE TITEI TE Operating Instructions Using the Imaging Tools Home File Preferences Home Clicking on this button will take you to the opening screen File Preferences Open Tiff File Save Image Export Image Print Print Preview Page Setup Copy Exit File Open Tiff Image Opens a saved
3. Installing Your BIS910 System This section is designed to help you quickly install your new BIS910 system Once you have completed these steps your BIS910 should be completely operational The first part reviews e Safety requirements maintenance and electrical ratings e Outofthe Box a visual list of the items in the product box e PC system requirements The second part details software installation Safety Requirements e Ensure that all devices are turned off before making any connections e Do not block the BIS910 s ventilation openings e Place the BIS910 at least 30cm 12 inches away from the walls and ceiling e Do not position the equipment so that it is difficult to perform device disconnection appliance coupler e Do not store below 10 C The recommended operating conditions for the BIS910 are 25 C 782F and 55 relative humidity The maximum ambient temperature is 28 C the maximum humidity is 80 e Transportation conditions Ship at 102 25 C e Equipment intended for indoor use only and up to an altitude of 2000m e Do not open the system Due to the danger of exposure to high voltage only trained service technicians should open the system e Warning The unit incorporates a source of hazardous UV radiation and should only be operated with a protective cover or a UV protective filter in place Otherwise there is a risk of exposure to radiation e Do not place the system near any motorized vibrating
4. Save settings Load settings Pseudo Color Invert Over Exposure Contrast Stretch optical enhancement Troubleshooting Before you contact support it is recommended that you read over this section to see if you can resolve the problem by yourself To make this as easy as possible we have created a list of the most common problems and their possible solutions I don t see any image on the screen Try one of the following e Make sure that the Transilluminator is turned on e Wait a few seconds Each time the settings change it takes time for the image to be displayed e f you are working with White Light then check that the iris is slightly closed Move the Iris dial toward the Close side to reduce the amount of light entering the iris If you are working with UV light the iris should be almost completely opened Move the Iris dial toward the Open side to increase the amount of light entering the Iris If it is a very dark sample the iris can be completely opened e Check that the lighting conditions are satisfactory The image is out of focus Check that the focus is set with its center mark aligned with the center mark on the BIS910 The frame counter is progressing but the picture is black e Check that the light on the Transilluminator table is switched on e Verify the iris position When working with UV light the iris should be almost completely opened If it is a very dark sample the Iris can be opened al
5. Show over exposure Area of Exposure time 0 01 sec Sensitivity 1 Brightness 1 j Y 3 Interest Set the Sensitivity Sensitivity is a feature which amplifies the signal emerging from your sample A higher GSensitivity setting will result in a brighter image As this feature amplifies any signal from the image you may see some background noise Since it is preferable to maintain a dynamic image range increasing the Sensitivity parameters will decrease the maximum exposure time Set the Brightness Increasing the Brightness will result in a brighter image Increasing the Brightness parameters will provide a less dramatically image brightness effect than the Gain Operating Instructions Saving the Image GelCapture allows you save images in TIFF BMP and JPEG formats You should be aware that tiff format saves all the raw data of the image and it is highly recommended to choose this format while intending to analyze a given image Other formats save only part of the image information and therefore provide less accurate results from image analysis process Saving options a Saving a single image Saving image in tiff format After determining the settings for an image and if you are happy with the results you should save the image by choosing one of the following buttons amp Print Export Analysis Edit Image Multi images Acquisition File Preferences Open Tiff File ave u 5S Expo
6. brand products and service names are trademarks or registered trademarks of their respective holders This document may contain flaws omissions or typesetting errors no warranty is granted nor liability assumed in relation thereto unless specifically undertaken by DNR sales contract or order confirmation Information contained herein is periodically updated and changes will be incorporated into subsequent editions If you have encountered any error please contact DNR Contact us at 972 2 570 0818 USA Toll free number 1 866 300 4286 email support dnr is com Table of Contents Introduction Features and Benefits Topics Covered Hardware System Overview Installing BIS910 System Safety Requirements Maintenance Electrical Ratings Out of the Box Components and Accessories Minimum PC Requirements Hardware Setup 2 Configurations BIS910 Work Flow 19 GelCapture Installation Windows 7 GelCapture Installation XP Pro Camera Driver Installation Windows 7 Camera Driver Installation XP Pro GelCapture Overview Icons 21 25 28 37 40 Operating Instructions Insert the Sample Set the Lights Adjust the Lens and Preview the Image Setting Camera Exposure Sensitivity amp Brightness Saving the Image Capturing a series of images Open Image File Using Imaging Tools Inverted Image Pseudo color Over exposure Troubleshooting Glossary Introduction The BIS910 is a superior and affordable gel do
7. drive is selected below C Program Files DNR GelCaptureBI531OWrivers Choose MiniBIS USB camera 1352M and click Next Select the device driver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a T disk that contains the driver you want to install click Have Disk W Show compatible hardware Model Eg MiniBI5 USB camera 1352M This driver has an Authenticodeftm signature Tell me why driver signing is important After successfully completing the driver update the successful update window will be displayed Click Close Ke U Update Driver Software MiniBl5 USB camera 1352M Windows has successfully updated your driver software Windows has finished installing the driver software for this device MimiEI5 USB camera 1352M Close the Device Manager and Control Panel windows The BIS910 camera driver is now installed and ready for use Installing the Camera Driver for Windows XP Pro The camera driver is pre installed in the BIS910 If however the user finds that this is not the case he should install it by following these instructions Before installing the camera driver you must first install GelCapture software on your computer Turn the BIS9010 unit on Windows XP Pro automatically displays the Found New Hardware Wizard Choose No not this time and click Next Found New Hardware Wizard
8. light was turned on it automatically switches off as a safety precaution Fig 18 BIS910 Drawer Open 5 Close the drawer and door Operating Instructions Set the Lights The BIS910 has Trans WL and UV illumination Press the light selection switch on the front of the D Transilluminator to the appropriate position UW WL selection switch Fig 19 D Transilluminator WL UV Switch With the BIS910 you must also check that the Bypass switch at the rear of the D Transilluminator is set to the BIS910 position Interlocking cable port connecting to the BIS 9109 ON OFF BIS901 By Pass switch Fig 20 BIS910 Bypass Switch The D Transilluminator may be used as a stand alone system by setting the BIS910 Bypass switch to the Bypass option thereby allowing the user to turn the illumination on while the BIS910 is N te located next to the D transilluminator Warning since UV radiation is harmful the user must use appropriate safety equipment when operating the D Transilluminator without the BIS910 camera tower The user should also protect his eyes by using WL intensive illumination without the camera tower Operating Instructions Adjust the Lens and Preview the Image Positioning Even though the system uses professional lenses you do not need to be a professional photographer to capture great images Often when you capture a new image you need to make some adjustments after you see the initial res
9. or magnetic equipment e Verify that all devices are turned off before making any connections e Equipment protection can be impaired if equipment is used in a manner not specified by the manufacturer e Do not store the system in direct sunlight or in the direct air flow path of an air conditioner e Do not clean the system with harmful solvents Use only a soft cloth dampened with water e Dispose of all plastic bags and wrapping according to local environmental regulations and keep them away from children Maintenance In order to keep the BIS910 in good working order take the following steps e The BIS910 must be cleaned every time a sample is removed Use a soft non abrasive lint free cloth e To clean all parts of the BIS910 use a cloth dampened with water e Do not operate while wearing gloves with talcum powder e Calibrate the camera once a year as described in the Camera Calibration section Electrical Ratings 100 240 V 50 60 Hz 0 6 A Note Before performing maintenance on any part of the BIS910 turn off the power to the D Transilluminator and connected devices Out of the Box Components and Accessories BIS910 BIS910 1 7 5 3 4 Trans 5 GelCapture 6 Inter Volt DC Drawers 2 illuminator and GelQuant locking Power WL UV Power Supply Express Software Cable Supply Cable Fig 13 Components and Accessories Minimum PC Requirements In order to install and operate both the BI
10. while Setup installs GelCaptureBIS910 on your computer Extracting files C Program Files ONR GelCapture BISS10 GelCapture B1S910 exe A 0 0 0 0 Once installation has been completed click Finish to exit Setup a Setup GelCapture BIS9 10 Completing the GelCapture BIS910 Setup Wizard Setup has finished installing GelCapture 8152910 on your computer The application may be launched by selecting the installed icons Click Finish to exit Setup The GelCapture capturing software for Windows XP Pro is now installed and ready to use BIS910 Camera Driver Installation Installing the Camera Driver for Windows 7 The camera driver is pre installed in the BIS910 If however the user finds that this is not the case he should install it by following these instructions Before installing the camera driver you mustfirst install GelCapture software on your computer Go to the Windows Control Panel by clicking Start gt gt Control Panel Calculator A Computer 4 Sticky Notes m Control Panel SS Shipping Tool APS Viewer ee Windows Fax and Scan Devices and Printers Default Programs Help and Support va Remote Desktop Connection All Programs Shut down re Open the Hardware and Sound Adjust your computer s settings uls System and Security a Review your computer s status A Back up your computer Find and fix problems Network and Internet w View network s
11. 0 02 201315 18 File folds a Music J Bluetooth Exchange Folder 29 08 2012 22 17 File fold Pier 14 02 201310 36 File fold En 44 mr wa 4 98 BE Videos Filename Image bmp Operating Instructions Capturing a series of images In order to capture a series of images select Multi Images Acquisition Print Acquisition You need to set the number of images you wish to capture The exposure time is determined by moving the scales see image below By Exposure time Extended Detection Show over exposure Exposure time 11 29 sec Sensitivity 1 Brightness 1 Area of a sa 4 an Interest r BLE In addition it is possible to create a repetition rule by marking the Repeat every button The time is determined by the user Save Multi images Acquisition Operating Instructions Open an Image File You can open an image in two possible ways 1 Onthe opening screen select Open Image File see image below with red arrow Home File Preferences Setup View Files Open Image File lt e Acquire Image Select correct hardware configuration White Light Tray UV Light Tray Blue Light Tray Save Image Export Image Print Print Preview Page Setup Copy Exit Multi images Acquisition Operating Instructions When a user opens image images the following screen will be shown File Image 1 tif ataken 26 6 2014 11 54 8 Exposure 1 45 sec sit
12. BIS910 u ff NEO MEW PHA API PL Eastwin Life Sciences Inc koz a b site http www dnr is com Email support dnr is com Important Notice Copyright 2011 DNR Bio Imaging Systems Ltd All rights reserved The information contained in this document is proprietary and is subject to all relevant copyright patent and other laws protecting intellectual property as well as any specific agreement protecting DNR Bio lmaging Systems Ltd DNR rights in the aforesaid information Neither this document nor the information contained herein may be published reproduced or disclosed to third parties in whole or in part without the express prior written permission of DNR Such information is supplied solely for the purpose of assisting explicitly and properly authorized users of the BIS910 DNR reserves the right without prior notice or liability to make changes in equipment design or specifications The text and graphics are for the purpose of illustration and reference only The specifications on which they are based are subject to change without notice Information in this user guide is subject to change without notice Corporate and individual names and data used in examples herein are fictitious unless otherwise noted The software described in this user guide is furnished under license The software may be used or copied only in accordance with the written permission of DNR Bio Imaging Systems Other company and
13. Create shortcut Delete Rename Properties When the next window appears select Allow I trust this program know where it s from or l ve used it before to allow the program to run Click Next in the Welcome to the GelCapture Setup Wizard window ja Setup GelCaptureBIS910 x gt Welcome to the GelCaptureBIS910 Setup Wizard This will install B15910 1 0 on your computer It is recommended that you close all other applications before CL mil WIT Ic J Click Next to continue or Cancel to exit Setup The installation progress window will be displayed with the green progress bar in motion Installing Please wait while Setup installs GelCaptureB15910 on your computer Extracting files C Program Files ONR GelCapture BIS910 GelCapture B1S91 0 exe uuu r ts ssSSCSsS Once installation has been completed click Finish to exit Setup if Setup GelCaptureBI5910 Completing the GelCaptureBIS910 Setup Wizard Setup has finished installing GelCaptureB15910 on your computer The application may be launched by selecting the installed icons Click Finish to exit Setup The GelCapture capturing software for Windows 7 is now installed and ready to use Installing GelCapture for Windows XP Pro Insert the CD into your PC and click Next in the Welcome to the GelCapture BIS910 Setup Wizard window im Tr Wan LIFE ToT setup Gellaptu LO Installing Please wait
14. P JPEG formats these saving options are eliminated wn Some PC s lack the ability to automatically open 16 bit tiff files format as captured with the BIS910 If you want to save an image for other reasons than analyzing it you can save it in BMP JPEG format which can be opened in most of the PC s Saving the Image in BMP JPEG Formats If you would like to save the image in a lighter image size than the tiff format you can choose either BMP or JPEG formats You should be aware that DNR highly recommends analyzing only images which are saved in tiff format In order to save the image in these formats you need to select the Export Image option There are 2 possible ways to do so please see below image Home File Preferences Open Tiff File Print Preview Page Setup Copy Exit Be Edit Image E Multi images Acquisition No of images 20 gt M Repeat every 30 Sec A Now you can choose at the Save Dialog screen between the BMP and JPEG formats Organize v New folder 7 Favorites Documents library EE Desktop Includes 2 locations h Downloads Ej Recent Places T Dropha J Adobe Captivate Cached Projects 10 02 2013 15 24 File fold D Google Dive Application Notes 10 06 2014 14 41 File foldi Authorization letters 08 06 2014 10 37 File fold Arrange by Folder Name Date modified Type BN Libraries Biocompare leads 16 01 2014 11 50 File fold RSE J BIOLOGICAL INDUSTRIES 2
15. P or JPG file formats pers the image in the analysis software GelQuant Express Opens the edit screen Multi images Acquisition various options of capturing a series of images GelCapture Overview The BIS910 system requires that you configure and fine tune in order to optimally acquire an image of each different sample type This process involves the following steps 1 Pre capturing verification steps Transilluminator and camera switches turn on drawer type choice 2 Insert the Sample 3 Set the Lights 4 Adjust the Lens and Preview the Image Positioning 5 Set the Camera Exposure Sensitivity and Brightness 6 Save the image Additional actions Print Flat Field Analysis Using the White Light converter Save an Image in jpg and bmp formats Using the Imaging Tools Operating Instructions Insert the Sample The very first step inserting the sample on the BIS910 drawer is completed in a few quick steps To insert the sample 1 Turn on the D Transilluminator and the BIS910 camera 2 Check that the BIS910 Bypass switch is set to the BIS910 position 3 Select appropriate drawer for your sample stain for a fluorescence application you should use UV drawer for a sample which stains with visible dyes you should use the WL drawer Insert the drawer into the D Transilluminator 4 Pull open the BIS910 drawer and locate the sample on the center of the drawer If the transilluminator
16. S910 and the GelCapture software you must have the following e Processor Pentium Core Due 1 8 GHz e Memory minimum 1 GRAM recommended 2 GRAM e OS Windows XP Pro English version SP 3 OR Windows 7 Professional English version e Only PCs with a 32 bit operating system and local Administrator permission at a minimum for the software installation process After the installation there is no need of Administrator permission e Minimum monitor resolution 1024 x 768 pixels Port 1 free USB 2 0 USB 1 0 or 1 1 are not compatible Note User must set the PC settings to Never Hibernate Hardware Setup Fig 14 BIS91OHardware Configuration 6815910 CESSE T ld Fig 15 BIS910 Hardware Configuration Work Flow Choose appropriate tray open B15910 door and locate sample on the D Transilluminator Close door and turn lights ON Adjust camera capture seftingi and capture an image E eu Fig 16 BIS910 Work Flow Software Installation GelCapture Software Installation for Windows 7 Once you have set up the hardware you can install all of the required software To install GelCapture for Windows 7 proceed as follows Insert the CD into your PC and right click the GelCapture exe installation file Select Run as administrator Open Eg Run as administrator Troubleshoot compatibility Scan for Viruses Always available offline Restore previous versions send to b Cut Copy
17. Unknown device and select Update Driver Software EE Computer i e Disk drives Mu Display adapters 8 DVD CD ROM drives lt H Floppy disk drives ei Floppy drive controllers i S Human Interface Devices Eg IDE ATA ATAPI controllers a IEEE 1394 Bus host controllers 422 Keyboards JA Mice and other pointing devices ME Monitors a Network adapters y ip Other devices ETF Ports CC i M Processo Disable Uninstall ee System d Eh F Uniwersal Scan for hardware changes Launches the Update Driver Software Wizard for the selected device In the Update Driver Software window select Browse my computer for driver software O L Update Driver Software Unknown Device How do you want to search for driver software Search automatically for updated driver software Windows will search your computer and the Internet for the latest driver software for your device gt Browse my computer for driver software Locate and install driver software manually Select Let me pick from a list of device drivers on my computer G Update Driver Software DNR MiniLumi 14SOM Browse for driver software on your computer Search for driver software in this location l 7 Include subfolders Let me pick from a list of device drivers on my computer This list will show installed driver software compatible with the device and all driver software in the same cate
18. c light cut out The light only turns on following correct insertion of the drawer and after the BIS910 door is closed D Transilluminator e WL Tray g lt On Off WL UV Fig 4 D Transilluminator Front View Illumination Switch Hardware System Overview Rear View The back panel of the D Transilluminator holds the cable connections the Power On Off switch and the BIS910 Bypass switch pea wyda Interlocking cable port Power supply port D Transilluminator ON OFF switch Fig 5 D Transilluminator Rear View After connecting all the cables and setting the BIS910 Bypass switch to the appropriate position the user should then advance to the GelCapture operation section Operating the BIS910 Bypass When the D Transilluminator is used by itself as a standalone system the BIS910 Bypass switch should be set to the Bypass position before preparing for image acquisition When the BIS910 is combined with the D Transilluminator the BIS910 Bypass switch should be set to the BIS910 position Fig 6 BIS910 Bypass Switch Hardware System Overview The D Transilluminator may be used as a stand alone transilluminator Warning since UV radiation is harmful the user must use appropriate safety equipment when Note operating the D Transilluminator without the BIS910 camera tower The user should also protect his eyes when using WL intensive illumination without the camera tower Accessories Visible Dye Converter
19. cumentation system The simple user interface provides laboratory researchers with quick accurate image acquisition and results These two systems are table top gel imaging systems that support both fluorescent and colorimetric visible dye applications This Operator s Manual is designed to guide the operator through all the required steps in order to scan gel samples Once you are more familiar with the system you may want to refer to The BIS910 Quick Reference Guide which is a more condensed version of this guide intended for experienced users Features and Benefits The key features that make the BIS910 the choice for life science researchers include Real time image viewing and analysis Scientific high resolution camera Compact safe and easy to use Superior optics with super bright lens which brings to ultimate results Unique D Transilluminator with two dedicated drawers for Fluorescence and visible dye applications Introduction This User Guide is one of two documents packaged with your BIS910 system To better utilize your system read the next chapter Installing Your BIS910 System before proceeding to the rest of the guide Once your system is installed and operational you can then familiarize yourself with the system s different features and procedures Everything you need to know about your BIS910 system is included in this guide Pay special attention to the highlighted chapters below They conta
20. d installing the driver software for this device MiniBIS USB2 a Close the Device Manager and Control Panel windows The BIS910 camera driver is now installed and ready for use GelCapture Overview The GelCapture application seamlessly integrates with the BIS910 The application gives you complete control of configuring capturing and saving your sampled images for analysis The interface shows all of the features and tasks on the main screen Home File Preferences Setup View Files Open Image File Acquire Image Select correct hardware configuration White Light Tray UV Light Tray Blue Light Tray In the entry screen you are required to select between the type of light source you wish to use 1 UV 2 White Light 3 Blue Light In addition you can chose to open an existing image by clicking on the Open Image File Adjust focus and i s then select capture parameters amp Print gt Export Analysis rend White Light Tray Camera settings Show over exposure E ag Area of 1 gt Exposure time 0 04 sec Sensitivity 1 Brightness 1 Adjust focus and iris to Interest get recquired image E mr AA A AAA ee eee itd kak a d do Baii Pan kmh lc an ns Change Menu bar Toolbar icons and Function Buttons explained below Main UV Screen here e Toolbar Icons Allows quick access to frequently used functions Menu Bar The drop down menu c
21. ess Esc save the settings of the proceudre exposure time sensitivity and brighness Load Settings If you wish to work with a saved procedure clicking on Load Settings will set the settings of the proceudre for this current experiment exposure time sensitivity and brighness Pseudo Color Shows the image in pseudo colors Invert Presents an inverse version of the image Exposure Contrast Automatically increases the stretching of the image This option helps reveal low Optical The image presented both in UV and WL is processed through an algorithm Enhancement which eliminates the lens distortions Un checking the Optical Enhancement button will cancel the algorithm and will present the original image captured Home File Preferences NE ma NA About GelCapture 9 DNR Website About GelCapture displays the information about the software version you are working on DNR Website directs you to DNR s website Operating Instructions Inverted Image An inverted image shows the image with the color scale inverted light areas appear dark and dark ones appear light much like a negative This feature is useful in such scenarios as helping the user to decipher dark areas Inverted Image Inverted image inverts the color scale i e light areas appear dark and dark areas appear light much like a negative This feature is particularly useful when you need to decipher close bands To Invert an Ima
22. ge Invert s Click the Invert Image icon Click the icon again to revert to original view of image You can also view an Image in invert position by selecting Preferences gt Invert Home File Preferences gt gt gt Area Of Interest Full Screen Save settings Load settings Pseudo Color Over Exposure Contrast Stretch optical enhancement Pseudo color To View an Image in Pseudo color Pseudo Colors Click the Pseudo color icon m Click the Pseudo color icon again to revert to the original view of the image You can also view an Image in Pseudo color by selecting Preferences gt Pseudo Color Area Of Interest Full Screen Save settings Load settings Pseudo Color Invert Over Exposure Contrast Stretch optical enhancement Overexposure Overexposure occurs when the camera receives too much light which usually results in white or bright areas This phenomenon leads to inaccurate results when analyzing the image information In order to avoid this distortion GelCapture is equipped with the Overexposure tool which displays overexposed areas in red To View an Image Using the Overexposure Tool Click the Overexposure button El AP Sa GG Exposure 1 45 sec Senstivity 2 Bnahtness 0 MA Unclick the Overexposure button again to revert to the original view of the image You can also view an Image in over exposure by selecting Preferences gt Over Exposure Preferences Full Screen
23. gory as the device Choose MiniBIS USB camera 13S2M and click Have Disk G Update Driver Software MiniBIS USB camera 1352M Select the device driver you want to install for this hardware Select the manufacturer and model of your hardware device and then click Next If you have a lt 8 disk that contains the driver you want to install click Have Disk 4 Show compatible hardware Model Cal MiniBIS USB carnera 1352M ESI This driver has an Authenticode tm signature Tell me why driver signing 15 important H Insert the manufacturers installation disk and then y2 make sure that the correct drive is selected below Copy manufacturer s files from Browse the following location Computer gt Local Disc C gt Program Files gt DNR gt GelCaptureBIS910 gt drivers gt PgrUsb and click Open E Recent Items FE Desktop chi Network Libraries S maor d Homegroup j Computer 05 C pe Program Files u DNR Lo GelCaptureB15910 whi drivers _ DVD RW Drive D 232933 on highleam 2002 cet ac il 233038 on highleam 2002 cet ac jl My Web Sites on MSN Choose MiniBIS from the PGRUSBCam inf and click Open H Locate File EE Look in MiniBIS E Bi Er Hr l Date modified ab Recent Places 4 File name Mini BIS Files of type Setup Information inf Click OK Insert the manufacturers installation disk and then make sure that the correct
24. image Save Image Saves newly scanned images Export Image Saving image in BMP JPG Print Prints the image Print Preview Allows you to preview the print before printing Page Setup Allows you to arrange the print before printing Copy Copies the image to the clipboard for pasting in any number of Window applications Exit Exits the software Operating Instructions Preferences Area Of Interest Full Screen Save settings Load settings Pseudo Color Invert Over Exposure Contrast Stretch optical enhancement Preferences Area of interest Defines an area of interest A green frame will appear on screen as seen in the below image Adjust the image size or position You can either stretch the pivot points on the frame or mouse left click and drag down to open a new frame of a region of interest Place the cursor anywhere within the green frame and right click to display the menu options A green frame will appear on screen as seen in the below image Adjust the image size or position You can either stretch the pivot points on the frame or mouse left click and drag down to open a new frame of a region of interest Place the cursor anywhere within the green frame and right click to display the menu options peceocecequereeeeer NOKEEEKEE LEEREREAJK zd Een E _ a Z M au oe m m obra iment main screen pr
25. in information that you may need to refer to in your day to day use of the BIS910 system Topics Covered Chapter An introduction to the manual and the topics covered Introduction 1 An introduction to the BIS910 and the GelCapture software Hardware and software 2 Hardware installation instructions and configurations Installation procedures 3 Work Flow BIS910 4 Installation procedures Software and drivers 5 Capturing saving imaging Step by step Operating Instructions tools etc 6 A listing of the most common issues and how to resolve each Troubleshooting 7 A list of terms used in this guide and their definitions Glossary 8 Hardware System Overview Each compactly designed BIS910 contains a high resolution camera and GelCapture software to provide the perfect solution for high quality imaging results The BIS910 Imager is a user friendly high sample imaging tool The BIS910 is constructed of two components a camera tower and a D Transilluminator which provides UV and White Light lower illumination GelCapture also previews and saves the scanned images which are then exported for analysis Configurations can also be saved and later recalled for use with future samples The BIS910 Unit The BIS910 comprises of the following main components Tower D Transilluminator Fig 1 BIS910 Hardware System Overview BIS910 Camera Tower The BIS910 camera tower holds a high resolution camera It connects directly to a PC
26. iv Brightness 7 Show over exposure El Print gt Export E Analysis Area of Intreset r OOO atak f Copy H Crop IT SALES TT ORLE NN ee T T s M Le Invert Kaj N 5 I Pseudo Colors MT 3 E E y 2 O Auto contrast gt Restore Original AJ Operating Instructions The Area of Interest feature lets you define a specific area of the Area of Intreset l sample in order to perform various actions on itfggtgttgt la Copy Copies the image to the clipboard for pasting in any number of Window applications Defines a specific Region of Interest in the Preview Screen in order to perform various actions on a specific area of the sample either in Live Mode or in a saved image de kai Presents an inverse version of the image IS Pseudo Colors Shows the image in pseudo colors Auto contrast Automatically increases the sIetching of the image This option helps reveal low concentrations of biological samples in the gel Restore Original Restores any stretching actions to the original image values Digitally zooms in and out to the image Displays the image so that it fills the entire screen To return to the GelCapture main screen press Esc Presents the image in its actual size Resizes the image to fit within the image window EC LITE TIES TELEN zaa sa NAC C MIL Qu WE di M BALU TI SHE BEI REII i re en ene HiME BRI DI
27. l the way The image quality is poor Recalibrate the camera as explained in the Camera Calibration section of the Installation Guide Please note that this takes at least 40 minutes to complete Glossary CCD The CCD charge coupled device is a major piece of technology in digital imaging In a CCD image sensor pixels are represented by capacitors These capacitors are based above the threshold for inversion when image acquisition begins allowing the conversion of incoming photons into electron charges at the semiconductor oxide interface the CCD is then used to read out these charges Although CCDs are not the only technology to allow for light detection CCD image sensors are widely used in professional medical and scientific applications where high quality image data is required Dynamic Range The range that a sensor is capable of differentiating If the sensor can differentiate between two points in an image then each point is translated into a pixel of the image if the sensor is not able to differentiate then the two points will be pixels of noise Dynamic Range is also referred to as Signal to Noise Ratio SNR Exposure Time The length of time that light is allowed to pass through to the camera lens to form an image Fluorescence The process whereby a fluorescent molecule is excited at one wavelength and immediately re emits it at another longer wavelength Gain Gain is a camera feature which increases or decreases
28. ontains all of the software features h El Print Export Analysis GelCapture Overview Area of Interest Exposure time 1 sec sensitivity 1 Briehtness 1 e Exposure Refers to the image exposure time This is the time available for the camera sensor to collect emitted light from the sample to form an image Increased exposure time means more time for light to reach the camera sensor to display sections of the image that were not visible with a lower exposure time e Sensitivity When the sensitivity is changed by moving the slider it will change the image lightness As a result of the Gain changes the maximum exposure time will be automatically changed accordingly e Brightness When the Brightness is changed by moving the slider it will change the image lightness it acts more sensitively than the Sensitivity parameters e Area of Interest Defines a specific Region of Interest in order to perform various actions on a specific area of the sample either in Live Mode or in a saved image Focus amp Ins recommended position Adjust focus and iris to get recquired image Please adjust the focus and iris for each image GelCapture Overview Icons The most common tasks and features can usually be accessed with a single click of a button Ee gt Fdit Image Multi images kaj min Saves newly scanned images amp Print The image will be printed gt Export Expoerts the image to BM
29. rt Invert Over Exposure Contrast Stretch optical enhancement Edit Image Multi images Acquisition Close position the iris is close and minimum light will go from the sample to the camera Open position The iris is open and maximum light will go from the sample to camera Focus Adjust this dial in order to obtain a fully focused image For professional focus adjustment you can use the Histogram bar in the Edit screen see image below pointed with a red arrow Slide the focus slide bar till you obtain the ultimate Edge number Adjust the focus until the Edge reading is at its highest The higher the number the sharper the image The user should be aware that every image and every sample in a given illumination intensity will have a different Edge number If you are not happy with the results you can revert the results received after adjusting the histogram by selecting Restore Original see image below pointed with a green arrow Home File Preferences Setup Capture a new image File Preview Image Date taken 25 6 2014 15 2 57 Exposure sec Sensitivity 0 Brightness 1 Show over exposure X X Value m u 8 039 ii Histogram Bar Operating Instructions Set the Camera Exposure Sensitivity and Brightness Set the exposure time by moving the sliders to the appropriate exposure time until you can clearly detect your sample By Exposure time Extended Detection
30. rt Image Print Print Preview Page Setup Copy Exit Multi images Acquisition Clicking on each one of the above buttons will display a pop up save image dialog as shown below o Save As B Libraries Documents Organize w New folder Documents library Includes 2 locations sk 4 Libraries Arrange by Folder gt 4 Documents _ eh Mic Name Date modified Type gt ee Pictures Adobe Captivate Cached Projects 10 02 2013 15 24 File fold gt Videos Application Notes 10 06 201414 41 File fold k Authorization letters 08 06 2014 10 37 File fold gt ly Homegroup M Biocompare leads 16 01 2014 11 50 File foldi gt BIOLOGICAL INDUSTRIES 20 02 201315 18 File fold a E Computer Je Bluetooth Exchange Folder 29 08 2012 22 17 File folch P 05 C Le Budget 14 02 201310 36 File fold a f CH 1737333 on hig Ki m 4 ik FMA FELG aan man FL FBI i Pk no File name Image tif Save as type TIFF Files tif Hide Folders You should provide an image name at the File name section Operating Instructions 1 The tiff format saves all the image raw data As a result the image sizes are slightly bigger several MB If you would like to save the image in a lighter format save it as BMP JPEG format You should be aware that while using analysis software it is highly recommended to use images in tiff format to gain accurate results Notes While saving images in BM
31. tatus and tasks Choose homegreup and sharing options Hardware and Sound View devices and printers s Adda device kal Programs a Uninstall a program View by Category User Accounts and Family Safety EP Add or remove user accounts fig Set up parental controls for any user Appearance and Personalization Change the theme Change desktop background Adjust screen resolution Clock Language and Region Change keyboards or other input methods Ease of Access Let Windows suggest settings Optimize visual display Open the Device Manager under Devices and Printers Pr Devices and Printers SA Adda device Add a printer Mouse ei Device Manager Connect the USB 2 0 imaging system between the system and the PC and turn on the imaging system camera The device manager should recognize the new device connection and alert you G L Computer xy Disk drives m9 ME Display adapters DVD CD ROM drives He Floppy disk drives E 8 Floppy drive controllers Human Interface Devices H E IDE ATA ATAPI controllers G IEEE 1394 Bus host controllers E gt Keyboards B M Mice and other pointing devices Monitors G Y Network adapters p Other devices ip Unknown device a ulv cl a Processors E 4 Sound video and game controllers lt gt Storage controllers 7 e System devices Aj Universal Serial Bus controllers Under Other devices right click
32. the strength of an electrical signal When the gain is increased the image s brightness also increases but so does any electronic noise This results in a brighter image with less clarity Glossary Iris A mechanical diaphragm which adjusts the lens aperture When there are high levels of illumination then iris should be closed and when there are low levels of illumination the iris should be opened Pixel A pixel or picture element is the basic unit that the camera uses to build an image Image size is also measured in pixels Pseudocolors Illustrates the levels throughout the sample s image The cooler colors such as blue indicate where the image is undersaturated and the hotter colors such as yellow or red indicate where the image is oversaturated Green indicates the levels are in the mid range Quantum Efficiency A measure of the efficiency by which photons generate electrons in a pixel The Q E is function of the camera sensor in regards to wavelengths Resolution Although definitions vary the most common one is the number of pixels on a camera
33. through its attached 2 0 USB compliant cable The camera tower sits on the D Transilluminator A vertical empty chamber extends directly below the camera providing the measured distance between the camera lens and the D Transilluminator where the sample image is captured BIS910 Camera tower rasiluminato Fig 2 BIS910 In order to prepare the BIS910 for operational use the user must connect the following cables e The USB 2 0 cable from the camera tower to the PC e The Interlocking cable between the camera tower and the D Transilluminator e The D Transilluminator Power supply cable from the D Transilluminator to the electrical socket Hardware System Overview D Transilluminator Fig 3 D Transilluminator The D Transilluminator can be used by itself as a standalone system or with the camera tower The D Transilluminator is provided for use with two interchangeable drawers one for white light WL and one for ultraviolet UV light However when the BIS910 operates on Dual UV see details on Page 15 below the UV drawer must be used with a visible dye converter see Visible Dye Converter on Page 14 below The Light Selection Switch is located on the front panel of the D Transilluminator This lower D Transilluminator unit is designed for maximum safety ensuring that personnel are not exposed to harmful light This integral safety feature assures you that if the BIS910 door or drawer is opened there is automati
34. ults For this reason we recommend that you start by previewing the image making the necessary adjustments and then getting more previews until you are happy with the results To preview the image L 2 Perform all of the required steps to prepare the sample Activate the GelCapture software by double clicking on the software shortcut or by using the start 4 ry menu Cl AM The opening screen appears and asks you to define the drawer type Home File Preferences Setup View Files Open Image File Acquire Image Select correct hardware configuration White Light Tray UV Light Tray Blue Light Tray Operating Instructions The software will be opened in Preview position Home File Preferences El Print Export E Edit Image Multi images Acquisition White Light Tray camera sotn Seno in Exposure time 0 04 sec Sensitivity 1 Brightness 1 Area of Adjust focus and iris to gt j i Interest get recquired image 8888 Operating Instructions While you are in Preview mode finger tip adjust the following dials which are located in the BIS910 camera tower Iris Adjust it till you reach satisfactory image illumination It is recommended to activate the Over Exposure icon see Page 80 for using over exposure Home File Preferences Area Of Interest Full Screen Save settings Load settings Pseudo Color Print Expo
35. welcome to the Found New Hardware Wizard Windows will search for current and updated software by looking on your computer on the hardware installation CD or on the windows Update web site with pour permission Read our privacy policy Can windows connect to windows Update to search for software O Yes this time only Yes now and every time connect a device Click Next to contmue Please wait while the wizard mstalls the software Eo DNA Camera LJ If the Windows Logo testing window is displayed choose Continue Anyway Hardware Installation A The software you are installing for this hardware DNA Camera has not passed Windows FE ech to ve ay with windows AP Tel me why this portant Continuing your installation of this software may impair or destabilize the correct operation of pour system either immediately or in the future Microsoft strongly recommends that you stop this installation now and contact the hardware vendor for software that has passed Windows Logo testing Cornus Anyway ETOP irate Once the Wizard completes the driver installation click Finish Found New Hardware Wizard Completing the Found New Hardware Wizard The wizard has finished installing the software for DNR Camera Click Fintsh to close the wizard pdate Driver Software MiniBIS USB2 Windows has successfully updated your driver software Windows has finishe
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