Sample manager troubleshooting
Contents
1. Leak in binary solvent manager Troubleshoot sample management system Degraded contami nated or improperly prepared sample Use fresh sample Column contaminated Clean or replace column Loss of column efficiency Clean or replace column2. Call Waters Technical Service Ambient temperature range error value Set room temperature to operational temperature range Battery backed file system reset value Call Waters Technical Service Battery backed memory h w failure Call Waters Technical Service Column heater over temperature or h w fault value Call Waters Technical Service Column heater cooler over temperature or h w fault value Call Waters Technical Service Column temperature range error value Set temperature to within method parameters Sample manager alarm messages Continued Message Resolution Column temperature Call Waters Technical Service sensor h w fault value Diagnostic test inter Displayed for information only No action is rupted by user required Drawing sample rate Set lower draw rate excessive value eCord connected Displayed for information only No action is required eCord data invalid Replace the eCord eCord disconnected Displayed for information only No action is required eCord driver h w fault Call Waters Technical Service eCord h w fault Ensure the eCord fob is attached to the receptacle on the side of the column manager eCord not present Attach the eCord fob to the receptacle on the side of the column manager eCord sw fault Call Waters Technical Service Sample manager alarm messages Continued Me
3. Switch 3 Out Allows control of third party instruments Switch 4 Out Allows control of third party instruments Run Stopped Out Indicates that the sample manager stopped oper ation because of an error condition or an operator request clicking Stop Flow button for example Changing rear panel interface connections Via the rear panel display you can open and close certain output connections Opening or closing output connections can be useful when you need to start or stop an injection or troubleshoot system connectivity To change sample manager rear panel interface connections 1 In the console select Sample Manager from the system tree 2 In thesample manager information window click Troubleshoot gt Rear Panel 3 In the Sample Manager Rear Panel dialog box locate a signal connection with an Out label and then click the red or green LED The output signal for the selected signal connection is either opened or closed J Sample organizer troubleshooting Power LED The power LED on the left side of the sample organizer s front panel indicates when the instrument is powered on or powered off Run LED The run LED to the right of the power LED indicates the run status Sample organizer run LED indications LED mode and color Unlit Description Indicates the sample organizer is currently idle Steady green Indicates the sample organizer is operating normall
4. freezes Possible cause Sample compartment temperature too cold Corrective action Defrost sample manager then choose a sample compartment temperature 8 C 46 4 F or greater Sample compartment door opened too frequently Minimize sample door access Alarm messages The following table lists the sample manager alarm messages and their resolutions To clear alarm messages 1 Follow the corrective action in this table and reset the sample manager via the control panel or console 2 Ifthe alarm message still appears on the console after you reset the sample manager reboot the sample manager If the alarm message persists contact Waters Technical Service Sample manager alarm messages Message Air sensor calibration h w fault Resolution Possible cause Air in system Resolution 1 Prime sample manager 2 Characterize needle and sample loop volumes Possible cause Damaged volume detection device tube Resolution 1 Inspect volume detection device tube for damage 2 Replace if necessary Possible cause Damaged volume detection device electrical line Resolution 1 Inspect volume detection device cable for damage 2 Replace if necessary Sample manager alarm messages Continued Message Air sensor calibration out of range value Resolution Call Waters Technical Service Air sensor volume not calibrated
5. Call Waters Technical Service Peak not found D2 656 nm Flowcell Bubbles Try Calibrate Flush flow cell and or replace lamp Peak not found xxx x Erbium 257 nm F lowcell Bubbles Try Calibrate Flush flow cell Peak not found xxx x Erbium 379 nm F lowcell Bubbles Try Calibrate Flush flow cell TUV detector alarm messages Continued Message Peak not found xxx x Erbium 521 nm F lowcell Bubbles Try Calibrate Resolution Flush flow cell Remote command receive while calibrating Wait for calibration to complete Displayed for information only No action is required Scan not found Defaults Set Call Waters Technical Service System cannot respond Call Waters Technical Service System not calibrated Try to Calibrate Recalibrate the detector This is not an ACQUITY TUV personality card Call Waters Technical Service Wavelengths span 370nm Order Filter not in use for nm XXX Displayed for information only No action is required Displaying TUV detector rear panel interface connections You can use the console to determine the status of input output signal connections on the rear panel of the TUV detector This display gives you a real time status of the instrument s signal connections A green LED indicates a signal cable is connected to the terminal A red LED indicates no signal cable is connected to the terminal To
6. Corrective action 1 Inspect line cord connections 2 Test electrical outlet for power Open or defective fuse Replace fuse Calibration or energy error on startup Nosolvent flowing through flow cell UV absorber in flow cell Make sure that solvent is flowing through the flow cell Ensure the mobile phase is transparent methanol or water Flush the flow cell Perform manual calibration Communication problems Configuration problem Check Ethernet configuration Bad cable Replace the cable TUV detector troubleshooting Continued Symptom Deuterium lamp does not light lamp LED is off solid red or blinking red or green Possible cause Faulty lamp Corrective action Replace the lamp Lamp not plugged in Plug in the lamp connector Bad lamp power supply board Contact Waters Tech nical Service Lamp switch in off position Inspect the rear panel connections or look for a timed event within a method possibly programmed to extin guish the lamp In dual wavelength mode time scale is incorrect Data rate on controller gt 2 point sec Use a data sampling rate of 1 or 2 point sec TUV detector troubleshooting Continued Symptom Nosample and refer ence energy lamp LED is off solid red or blinking red or green Possible cause Lamp burned out Corrective action Attempt to rei
7. Life Time Defaults Set Call Waters Technical Service Lamp energy low Replace lamp Lamp external input conflict Set the input on the rear panel so that it does not conflict with the lamp command Lamp failure Replace lamp Lamp lighting failure Replace lamp Light Level Error Air in Cell Try to Calibrate Flush flow cell TUV detector alarm messages Continued Message Light Level Error Ref Saturated Cycle Power Resolution Flush flow cell Lowered gain to avoid saturation Ref Light Level Increased Calibrate the detector Lowered gain to avoid saturation Sample Light Level Increased Displayed for information only Indicates that flow cell bubbles were cleared No action is required Method not found Defaults Set Call Waters Technical Service Mux ADC Calibration command failure Call Waters Technical Service Mux ADC default register error Call Waters Technical Service Mux ADC input selection failure Call Waters Technical Service Mux ADC reset command failure Call Waters Technical Service TUV detector alarm messages Continued Message Nointerrupt from preAmp board Average Samples Resolution Call Waters Technical Service Nointerrupt from preAmp board Lock Cannot Continue Call Waters Technical Service Nointerrupt from preAmp board Waiting for Data in Dual
8. for information only No action is required Calibration unsuccessful 257 Peak out of range Flowcell Bubbles Flush flow cell Calibration unsuccessful 379 Peak out of range Flowcell Bubbles Flush flow cell Calibration unsuccessful 521 Peak out of range Flowcell Bubbles Flush flow cell Communication failure Reference or Sample A D Call Waters Technical Service TUV detector alarm messages Continued Message Resolution Configuration not found Call Waters Technical Service Defaults Set Dark current Reference Close detector door Dark too high Filter initialization failure Erbium position Clear bubbles from flow cell Filter initialization failure No filters found Clear bubbles from flow cell Filter initialization failure No reference energy Clear bubbles from flow cell Filter initialization failure Order filter position Clear bubbles from flow cell Filter initialization failure Shutter position Clear bubbles from flow cell TUV detector alarm messages Continued Message Grating initialization failure Backlash too high Flowcell Bubbles Try Cal Resolution Clear bubbles from flow cell Grating initialization failure Missing Bad Sensor Call Waters Technical Service Lamp data not found Life Time Defaults Set Call Waters Technical Service Lamp data not found
9. panel interface connections You can use the console to determine the status of input output signal connections on the rear panel of the sample manager The console display gives a real time status of the instrument s signal connections A green LED indicates a signal cable is connected to the terminal A red LED indicates no signal cableis connected to the terminal To display rear panel interface connections 1 In the console select Sample Manager from the system tree 2 In thesample manager information window click Troubleshoot gt Rear Panel The Sample Manager Rear Panel dialog box appears Sample Manager Rear Panel dialog box Sample Manager Rear Panel Inject Start Out Switch 1 Out Auxiliary In Switch 2 Out Ground Switch 3 Out Hold Inject In Switch 4 Out Ground OO NDR WN EE 1 2 3 4 5 6 7 8 9 0 2 Y Analog Out Run Stopped Out o Close The following table describes the sample manager I O connections Analog out event in connections Signal connections Inject Start Out Description Indicates that an injection has started Auxiliary In Reserved for future use Inject Hold In Input signals from other system instruments to delay the injection 0 2V Analog 2 Out Chart output functionality Switch 1 Out Allows control of third party instruments Switch 2 Out Allows control of third party instruments
10. solvent manager tion times head M alfunctioning check Clean or replace inlet check valve val ves cartridges Leaking plunger seals Replace seals Separation chemistry Determine the suitability of mobile phase and column Clogged solvent filters Replace filters Equilibration timeafter Use the column manager a gradient may be insufficient Chromatography troubleshooting Continued Symptom Increased reten tion times Possible cause Incorrect flow rate Corrective action Change flow rate Incorrect solvent composition Change solvent composition Incorrect mobile phase Use correct mobile phase Column contaminated Clean or replace column Incorrect column Use correct column Fluid leak causes lower flow rate Inspect fittings for leaks Perform leak test Chromatography troubleshooting Continued Symptom Decreased reten tion times Possible cause Incorrect flow rate Corrective action Change flow rate Incorrect solvent composition Change composition High column temperature Reduce column temperature Incorrect mobile phase Use correct mobile phase Column contaminated Clean or replace column Incorrect column Use correct column Sample diluent may be stronger than the initial mobile phase Dilute sample in a weaker solution Inject less Rep
11. LED indicates the lamp status TUV detector lamp LED indications LED state Unlit Description Indicates the detector is currently idle Steady green color Indicates the detector is operating normally Flashing green color Indicates the detector is initializing or calibrating Steady red color Indicates a serious error that prevents further operation Information about the error can be found in the console TUV detector lamp LED indications Continued LED state Description Flashing red color Indicates a serious failure within the detector that prevents further operation Clearing bubbles from the flow cell To clear bubbles from the flow cell 1 Ensure the pressure regulator at the outlet of the flow cell isin place If the pressure regulator has been bypassed ensure that a device that develops a minimum of 17 bar 250 psi but not greater than 69 bar 1000 psi for the given flow rate and mobile phase is placed downstream from the detector 2 Establish liquid flow through the detector flow cell with degassed acetonitrile or methanol at a flow rate that is anticipated to be used for subsequent analysis TUV detector troubleshooting Symptom Possible cause Corrective action Analog output incorrect AUFS setting changed Reset the AUFS setting TUV detector troubleshooting Continued Symptom Both LEDs unlit Possible cause No power
12. Sample manager troubleshooting Power LED The power LED on the left side of the sample manager s front panel indicates when the instrument is powered on or powered off Run LED The run LED to the right of the power LED indicates the run status Sample manager run LED indications LED mode and color Unlit Description Indicates the sample manager is currently idle Steady green Indicates the sample manager is operating normally attempting to complete any outstanding samples or diagnostic requests When sample and diagnostic requests are finished the LED returns to the unlit state Flashing green Indicates the system is waiting for an instrument to reach operational condition as when the column manager is reaching its set point Sample manager run LED indications Continued LED mode and color Flashing red Description Indicates a serious error that prevents further operation Information about the error can be found in the console Steady red Indicates a serious failure within the sample manager that prevents further operation Sample manager troubleshooting Symptom Both LEDs unlit Possible cause No power Corrective action 1 Inspect line cord connections 2 Test electrical outlet for power Open spent or defec tive fuse Replace fuse Sample manager troubleshooting Continued Symptom Sample compartment
13. anagement system or sample management system P ower off and power on again If failure persists call Waters Technical Service Alarm messages The following table lists the sample organizer alarm messages and their resolutions To clear alarm messages 1 Follow the corrective action in this table and reset the solvent organizer via the control panel or console 2 Ifthe alarm message still appears on the console after you reset the sample organizer reboot the sample organizer If the alarm message persists contact Waters Technical Service Sample organizer alarm messages Message Resolution Organizer communica Check external cable connections tions failure Organizer configuration Reconfigure the shelves through the console modified Organizer failure Call Waters Technical Service Organizer not present Check external cable connections Sample organizer alarm messages Continued Message Organizer plate does not exist Resolution Ensure sample plate is in the organizer Organizer processes interrupted Restart the process that was interrupted Sample Organizer temper ature range error value Call Waters Technical Service TUV detector troubleshooting Power LED The power LED on the left side of the detector s front panel indicates when the instrument is powered on or powered off Lamp LED The lamp LED to the right of the power
14. ctor Troubleshoot detector The mobile phase might absorb too much at the wavelengths selected Do not monitor at such low wavelengths Lower the concentration of the additive Use a different additive or solvent with less absorbance and the chosen wavelength Chromatography troubleshooting Continued Symptom Flat baseline no peaks Possible cause Nosolvent flow Corrective action Determine flow rate Detector lamp not on Use detector diagnostics to determine reference sample energy Zero energy indi cates lamp not on Power on lamp If this does not resolve the problem replace the lamp Detector not zeroed Zero the detector baseline Incorrect detector wavelength Check wavelength setting Leak in solvent path Check fittings The mobile phase might absorb too much UV at the wavelengths selected Do not monitor at such low wavelengths Lower the concentration of the additive Use a different additive or solvent one with less absorbance at the chosen wavelength Refer to Appendix D in the Waters ACQUITY UPLC System Operator s Guide Chromatography troubleshooting Continued Symptom F lat topped peaks Possible cause Sample concentration or injection volume exceeds voltage output of detector Corrective action Decrease sample concentration or injection volume Sensitivity loss
15. display TUV detector rear panel interface connections 1 In the console select TUV Detector from the system tree 2 Click Troubleshoot gt Rear Panel The TUV Detector Rear Panel dialog box appears TUV Detector Rear Panel dialog box TUY Detector Rear Panel Analog 1 Out Ground st ik Analog 2 Out Switch 1 Out Ground A el A el Switch 2 Out tl t pol Inject Start In Ground Lamp On Off In Chart Mark In Ground Auto Zero In Close The following table describes the TUV detector I O connections TUV detector analog out event in connections Signal connections Description Analog 1 Out Used for analog chart output functionality TUV detector analog out event in connections Continued Signal connections Analog 2 Out Description Used for analog chart output functionality Switch 1 Out Controlled by threshold and timed events Switch 2 Out Controlled by threshold and timed events Inject Start In Activates timed events by triggering the run time clock to start Lamp On Off In Enables and disables the lamp When the input is active the lamp is on Chart Mark In Adds a chart mark at 10 of full scale to either or both analog output channels Analog Out 1 and Analog Out 2 and is configurable Auto Zero In Calculates an offset value that when added to the sample signa
16. gnite the lamp If it fails to ignite replace the lamp Lamp off Ensurethe lamp switch is in the on position Run the Sample and Reference Energy diagnostic Peak out of range error at start up M obile phase absor bance too high or air bubbles in the flow cell Ensure the mobile phase in the flow cell does not absorb above 250 nm Ensure no air bubbles are in the flow cell Recalibrate the detector Alarm messages The following table lists the TUV detector alarm messages and their resolutions To clear alarm messages 1 Follow the corrective action in this table and reset the detector via the control panel or console 2 Ifthe alarm message still appears on the console after you reset the detector reboot the detector If the alarm message persists contact Waters Technical Service TUV detector alarm messages Message Resolution Calibration differs x xx Recalibrate the detector 257 nm Flowcell bubbles possible Try Calibrate Calibration differs x xx Recalibrate the detector 379 nm Flowcell bubbles possible Try Calibrate TUV detector alarm messages Continued Message Calibration differs x xx 521 nm Flowcell bubbles possible Try Calibrate Resolution Recalibrate the detector Calibration not found Defaults Set Call Waters Technical Service Calibration successful Max nm deviation since last calibration x xx Displayed
17. hooting Continued Symptom Change in reference spectrum Possible cause Mobile phase contains gas or is contaminated Corrective action Prepare fresh mobile phase and degas thoroughly Air bubbles trapped in flow cell Reseat and check align ment of flow cell Flush the flow cell or apply slight backpres sure on the detector waste outlet Ensure the backpres sure regulator is connected to the detector waste outlet 2996 PDA detector troubleshooting Continued Symptom Detector not responding to console Possible cause Detector not connected tolEEE to Ethernet Corrective action Inspect IEEE cable connections tighten converter connectors Incorrect IEEE address 1 Ensure that the detector IEEE address is unique and within therange 2 to 29 see the E mpower System Installation and Configuration Guide 2 Rescan the acquisition server properties F or details seeE mpower Help Shutter failure message Shutter failed Run the Shutter diag nostic For details see Empower Hdp 2996 PDA detector troubleshooting Continued Symptom Solvent in drain line Possible cause Leak from flow cell gasket Corrective action Replace the flow cell Leak from flow cell fittings Check fittings for over tightening or undertightening and replace fittings if necessary Status light blinks and lamp lig
18. ht is off The detector is running confidence tests No corrective action required 2996 PDA detector troubleshooting Continued Symptom Status light blinks and lamp light on Possible cause Failed startup diagnostics Corrective action Reseat and check align ment of flow cell Flush the flow cell Insufficient energy reaching photodiode array because of air bubble or dirty flow cell can cause shutter diagnostic to fail Flush the flow cell To prevent air bubbles from forming check that there is a 1 to 2 foot 30 to 60 cm length of 0 009 inch 0 23 mm ID tubing connected to the detector waste outlet Weak lamp Replace lamp Chromatography troubleshooting Warning To prevent injury always observe good laboratory practices when you handle solvents change tubing or operate the ACQUITY UPLC system K now the physical and chemical properties of the solvents you use Refer to the Material Safety Data Sheets for the solvents in use The table below lists chromatographic symptoms possible causes and suggested corrective actions for the ACQUITY UPLC system Before you use the table read the General Troubleshooting section and follow the basic troubleshooting steps to isolate the cause of the chromatographic symptom Chromatography troubleshooting Symptom Possible cause Corrective action Erratic reten Air bubble in pump Prime binary
19. l makes the resulting baseline signal zero a Toconfigure an inject start chart mark auto zero and lamp input set the appropriate parameter to High Changing the rear panel interface connections Via the rear panel display you can open and close certain output connections Opening or closing output connections can be useful when you need to start or stop an injection or troubleshoot system connectivity To change TUV detector rear panel interface connections 1 In the console select TUV Detector from the system tree 2 IntheTUV detector information window click Troubleshoot gt Rear Panel 3 IntheTUV Detector Rear Panel dialog box locate a signal connection with an Out label and then click the red or green LED The output signal for the selected signal connection is either opened gt or closed 2996 PDA detector troubleshooting The 2996 PDA detector runs a series of internal diagnostics on start up The indicator LEDs on the front of the detector and messages at the console show the results of the startup diagnostics Refer to the table to troubleshoot problems encountered during startup diagnostics and during detector operation 2996 PDA detector troubleshooting Symptom Both LEDs unlit Possible cause No power Corrective action 1 Inspect line cord connections 2 Test electrical outlet for power Open spent or defec tive fuse Replace fuse 2996 PDA detector troubles
20. nt no plate expected Remove plate from instrument Power supply fan h w fault Call Waters Technical Service Power supply h w fault value Call Waters Technical Service Sample fluidics high pres sure limit value Possible Cause Clogged fluid line volume detec tion device sample loop needle internal valve passages Resolution 1 Check for kinked or crushed tubing volume detection device sample loop and needle 2 Replace damaged tubing Sample heater cooler over temperature or h w fault value Call Waters Technical Service Sample manager alarm messages Continued Message Sample Manager door closed during defrost Resolution Open sample manager door Sample Organizer door closed during defrost Open sample organizer door Sample Organizer temper ature range error value Set temperature to within method parameters Sample manager alarm messages Continued Message Sample pressure low value possibly no wash solvent or needle damaged Resolution Possible Cause Wash solvents low or empty Resolution Refresh wash solvents and prime the sample manager Possible Cause Damaged needle Resolution Run Change Needle diagnostic and inspect needle for damage Replace if damaged Possible Cause Loose plumbing connection or leaking injection seal Resolution Verify fittings and ferrules are tigh
21. roducibility errors Incorrect chemistry integration Review chemistry and integration Sample management system problem Troubleshoot sample management system Chromatography troubleshooting Continued Symptom Baseline drift rapid Possible cause Column not equilibrated Corrective action E quilibrate column Detector not allowed to warm up Allow detector to warm up until baselineis stable Time varies with wavelength and sensitivity Solvent contaminated Use fresh solvent Flow fluctuations rapid or slow drift Prime pump replace pump seals check valves see Erratic retention times symptom earlier in this table Incorrect wavelength for solvent Ensure solvent does not absorb at the wavelength used Chromatography troubleshooting Continued Symptom Baseline drift slow Possible cause Solvent contaminated Corrective action Use fresh solvent Decreased UV lamp energy Determine lamp energy at the console Ambient temperature fluctuations Stabilize operating environment tempera ture enough to allow full equilibration UV detector flow cell leaking internal cross port Inspect flow cell tighten connections Dirty flow cell Clean flow cell Absorbance of A and B mobile phases are different due to spectral differences in the addi tives in water and organic solvent
22. s Balance the absorbance of the two mobile phases by reducing the concentration of the additive in the higher absorbing mobile phase Caution This makes the mobile phases slightly different with respect to the additive Chromatography troubleshooting Continued Symptom Baseline noise cycling short term 30 to 60 seconds Possible cause F low fluctuating Corrective action Look for leaks See Erratic retention times symptom earlier in this table Radio frequency noise short or long term cycling Eliminate interference Baseline noise cycling long term approxi mately 10 minutes to 1 hour Ambient temperature fluctuations Stabilize ambient temperature Chromatography troubleshooting Continued Symptom Baseline noise random Possible cause Air in detector Corrective action Purge detector to remove air Bubble detected Reprime the solvent management system Remove and vacuum filter solvents Solvents contaminated Use fresh solvent Column contaminated Clean or replace column Dirty flow cell Clean flow cell System improperly grounded Plug into outlet on different electrical circuit Use power conditioner Unit not cooling properly Operate unit with covers in place Check back panel for proper clearance Radio frequency noise Eliminate interference Defective dete
23. ssage Failed to calibrate system volume Resolution Possible cause Air in system Resolution 1 Prime sample manager 2 Characterize needle and sample loop volumes Possible cause Damaged volume detection device tube Resolution 1 Inspect volume detection device tube for damage 2 Replace if necessary Fuse h w fault Call Waters Technical Service General failure Call Waters Technical Service Home and or plate sensors h w fault Call Waters Technical Service Home and or reference sensors h w fault Call Waters Technical Service Injection count exceeded value Displayed for information only No action is required Sample manager alarm messages Continued Message Resolution Injection valve home h w_ Call Waters Technical Service fault Injection valve move h w _ Inspect the injection valve pod and ensure it is fault securely fastened to the valve drive Sample manager alarm messages Continued Message Loop calibration out of range value Resolution Possible cause Air in system Resolution 1 Prime the sample manager 2 Characterize needle and sample loop volumes Possible cause Clogged or kinked needle injector valve or loop Resolution 1 Inspect needle and loop for damage 2 Replace if necessary Possible cause Damaged volume detection device tube Resolution 1 Inspect
24. t and replace if necessary Possible Cause Pressure regulator out of range low Resolution Call Waters Technical Service Sample manager alarm messages Continued Message Sample pressure sensor h w fault value Resolution Possible Cause No power to transducer loose el ec trical connection Resolution Inspect transducer cable and ensure it is properly secured in fluidics interface board Possible Cause Zero float out of range Resolution Call Waters Technical Service Sample syringe h w lost steps value Call Waters Technical Service Sample syringe home h w fault Call Waters Technical Service Sample syringe move h w fault Call Waters Technical Service Sample syringe move to sensor h w fault Call Waters Technical Service Sample temperature range error value Set temperature inside method parameters Sample temperature sensor h w fault value Call Waters Technical Service Sample manager alarm messages Continued Message Sample valve home h w fault Resolution Call Waters Technical Service Sample valve move h w fault Call Waters Technical Service Temperature controller watchdog time out Call Waters Technical Service Trap valve h w communi cation fault Call Waters Technical Service Trap valve h w fault Call Waters Technical Service Trap valve not installed Install
25. trap valve Wash syringe h w lost steps value Call Waters Technical Service Wash syringe home h w fault Call Waters Technical Service Wash syringe move h w fault Call Waters Technical Service Wash syringe move to sensor h w fault Call Waters Technical Service Sample manager alarm messages Continued Message X amp Y axes not calibrated Resolution Calibrate the XYZ mechanism X axis h w lost steps value Call Waters Technical Service X axis home h w fault Call Waters Technical Service X axis move h w fault Call Waters Technical Service Y axis h w lost steps value Call Waters Technical Service Y axis home h w fault Call Waters Technical Service Y axis move h w fault Call Waters Technical Service Z axis h w lost steps value Insert correct plate Ensure plate is inserted correctly Z axis home h w fault Call Waters Technical Service Z axis move h w fault Call Waters Technical Service Z axis not calibrated Calibrate the XYZ mechanism Zp axis h w needle lost steps value Insert correct plate Ensure plate is inserted correctly Zp axis home h w fault Call Waters Technical Service Sample manager alarm messages Continued Message Zp axis move h w fault Resolution Call Waters Technical Service Displaying the sample manager s rear
26. volume detection device tube for damage 2 Replace if necessary Possible cause Incorrect loop configuration Resolution Change loop configuration on the console Sample manager alarm messages Continued Message Loop volume not calibrated Resolution Possible cause Change in configuration and loop not characterized Resolution Characterize needle and sample loop volumes Missing wrong plate or vial Check if correct plate is selected and if ANSI version of plate is selected Modified SN Displayed for information only No action is required Sample manager alarm messages Continued Message Needle calibration out of range value Resolution Possible cause Air in system Resolution 1 Prime the sample manager 2 Characterize needle and sample loop volumes Possible cause Clogged or damaged needle injector valve or loop Resolution 1 Inspect needle and loop for damage 2 Replace if necessary Possible cause Damaged volume detection device tube Resolution 1 Inspect volume detection device tube for damage 2 Replace if necessary Possible cause Incorrect needle configuration Resolution Change needle configuration on the console Sample manager alarm messages Continued Message Needle volume not calibrated Resolution Characterize needle and sample loop volumes Plate prese
27. y attempting to complete any outstanding samples or diagnostic requests When sample and diagnostic requests are finished the LED returns to the unlit state Flashing green Indicates the system is waiting for an instrument to reach operational condition Sample organizer run LED indications Continued LED mode and color Flashing red Description Indicates a serious error that prevents further operation Information about the error can be found in the console Steady red Indicates a serious failure within the sample organizer that prevents further operation Sample organizer troubleshooting Symptom Both LEDs unlit Possible cause No power Corrective action 1 Inspect line cord connections 2 Test electrical outlet for power Open spent or defec tive fuse Replace fuse Sample organizer troubleshooting Continued Symptom Fans do not run Possible cause Sample organizer not powered on Corrective action Power on sample organizer Fan wiring or fan motor problem Call Waters Technical Service Power supply not working Call Waters Technical Service Sample plates jam Plate latch is bent Remove plate latch from plate straighten it and then reinstall it If problem persists call Waters Technical Service Startup diagnostics failure Internal problem with controller board solvent m
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