User Manual
Contents
1. E II c The plate name will appear on the Input Stack 8 After the machine finish the operation change the care solution bottle with polymer bottle according to the software protocol and it s ready to run the sample list The Care Solution Run can now start 16 ES MCLAB After pot 1 650 872 0245 www mclab com 17 T T TT SR n i mi ni E pin Wi i Al i A E M T lj n 1002. CARE Solution ABI 3730XL User Manual 384 Oyster Point Blvd Suite 15 South San Francisco CA 94080 Phone 1 888 MCLAB 88 Fax 1 650 872 0253 www mclab com Care Solution Protocol 1 Create a new module a Open Foundation Data Collection Version 3 0 b Under GA Instruments gt ga3730 click on Module Manager in the left hand column c On the bottom of the window click New to set up a new module Foundation Data Collection Version 3 0 No User is logged in AB Bis perita incas Nos Vonage jesus croup casa versos Loon Elen iege Fun voue na e E 1 REGULAR ves 5 Gilt E Cgoxeoo seons REGULAR GeneMspper o ves Bremen PORTA tepa por 1 REGULAR vs ves 1 REGULAR vs Lanarun_Genemaoet REGULAR Fastsess0_POP7 ne Meira porra REGULAR Lonaseasu_por7 er KapltSonst 1 REGULAR v Seater SPATIAL peta yc Seatamor SesmaL pataNort 1 SPECTRAL Speco bed POP v POPT 1 SPECTRAL Spec POP7 1 SPECTRAL Ms ves Spec sonst FOP SPECTRAL Spec sed ves Se o POPT 1 REGULAR Ed XLRSeq50 POPT 1 REGULAR ves
3. close the main door and wait for the green status lightto illuminate Then click Fill Array If you choose notto fill the array atthis time proceed to step 2 Please wait approximately 2 minutes for the Fill Array procedure to complete 2 Remove the buffer jar rinse with purified water then fill itto the indicator line with 1X run buffer 3 Install the buffer jar on the lower polymer block then close the main door 4 Click Finish 4 amp MCLAB 6 Create a new sample sheet just for the wash a In the left hand column click on plate manager 6 Choose any template and click on Duplicate Change the name to For Wash then click on OK Found Fio AB pom Gros p cha pete onna Trese Eine Marea Typo of Soarch Maneger ai run story 4 ese 0102130 55 2013 01 02 20 13 09 over 2013 01 02 15 47 44 oaa 2012 01 02 18 15 48 D D213RCAtoct 2013 01 02 13 31 01 onozvarcanda WE 20201 02 1331 15 crono 2013 01 02 102205 or dores Nae 2012 01 03 133327 opos 2013 01 03 18 3039 010313 ai 2013 01 03 18 30 33 orbus 201301 04 144255 oras 2012 01 04 4430 orate 2012 01 04 211231 onorise 15457 ora 20 processed 2012 01 07 to 044 oue 26 processed 2012 01 07 211589 mona mora Semerinkahi
4. Epor 1650 872 0245 www mclab com 1 Run Module Editor Run Module Description Name for Was Type REGULAR Template Description Capillary wash only Value Range 60 18 70 Deg PreRun_Voltage E PreRun_Time 1 1800 sec Injection Voltage Injection Time First ReadOut Time y i Second ReadOut Time 100 16000 ms Voltage v 0 15 KV Voltage Number Of Steps 100 Steps Voltage Step Interval El 180 secs Current Stability c 000 uA Ramp Delay E 1800 sec 0 0 Voltage_Tolerance 0 6 0 KV 0 1 1 Data_Delay 1800 sec E Run Time q 300 14000 sec Lo 2 Setting Run Module Editor Values 2 Name the module For Wash 6 Under Type choose REGULAR c Under Template choose LongSeq50_POP7 d Name Description Capillary wash only e Adjust settings to match Value column listed above Click Ok 2 4 amp MCLAB 3 Creat a new run protocol a In the left hand column under GA Instruments ga3730 click on Protocol Manager 6 Click New IE Foundation Data Collection Version 30 No Usar e logged In BEE o Yon S A carris W ca uno rita croup Pa Sauna Menager T Lem ES Eu pectin POP 1 CJoxsao seaoo es ie DESO
5. PORTA Run Sere msc Spes Meet 1 EXE EX TONO Cay ws ay 3 a Long Lan Gees a Yor SpatalFilL 1 Spat alFillt Created with populator tar Corto emcee crested th soplete 1 ERES rc EE Em 5 lt ra 1650 872 0245 www mclab com Name Description Type Run Module Dye Set Protocol Editor Capillary wash only Z BigDyey3 4 Setting Protocol Editor Values a Name the Protocol For Wash b Name Description Capillary wash only c Under Type choose REGULAR d Under Run Module choose For Wash e Under Dye Set choose Z BigDyeV3 Click OK 4 4 amp MCLAB 5 Perform Change polymer Wizard simply follow the normal protocol From Run Scheduler screen go Change Polymer Wizard and click it Then we can go the procedure of Change Polymer Wizard b Step 1 choose Different Lot then Next EB Foundation Data Collection Version 3 0 No User is logged in w Instrument Se HAS ALGA instruments Bubble Remove Wizard results Group i Database Manager Water Wash Wizard a Instrument Shutdown Wizard Add Plat ilo Manager Input Stack E es Protocol
6. Mash mobil FOZ Wah melabt1 MCLab E Drive For Wash mclab11 MOLsbE rive Foch mobil MOD em MCLeb E Drive Fox Wash A03 em meia MoLab E Drve ih mobil B03 Drive Fox Wash mobil C08 Wah mclabi MCLab E Drive For Wash mclab11 00 Mab EOrve Fox ih mobil MCLab E Drive Foch Wash mclab11 MCLab E Drive For Wash mclab11 C NCLab E Drve Foch mobil lt gt Description 7 Schedule a run for the Wash program a Click on Run Scheduler in the left hand column and then click on Search A button Add Plates to Input Stack will appear 6 In the Add Plates to Input Stack window enter your plate name in the search area then click Search The plate name will appear on the bottom of this window Click Add then Done 1 650 872 0245 www mclab com 15 FE Foundation Data Collection Yersion 3 0 _ FE ea ERR Se EE No Usar logged in HAS Eolas Hone Find Stacker Pale Adel Rate Sean or Type DJ B Proiccai ew LTEM Add Plates to Stack viewer Blocs Viewer El of Search Barcode v Cortrol Soan or Type Plate D ram ES Search Resute Append Rezute RunD
7. Manager Output s Module Manager Plate 1D Plate Name Plate Type Run History mi El Cjoxezo seoos 5 E3 nstrument Status Es Run Schedule Run Scheduler capillary Viewer E ll Spectral viewer lt z 4 Manuel Control Up Auto Sampler Plate Name Plate Type Status Current Runs RunID Application Run Protocol Status 1650 872 0245 www mclab com Change Polymer Wizard Step 1 Select Polymer Lot Type NOTE Always wear appropriate protection when handling the fluids used on the instrument To change polymer you will need Bottle of polymer 1 Is the lot of polymer to be installed the same or different from the currently installed lot O Same Lot Click Next Step 2 enter and the expiration date Finish 6 MCLAB Change Polymer Wizard Step 2 Enter Polymer Information 1 Enter the polymer lot information Polymer Type Polymer lot number care Polymer expiration date mm dd yy or daMMMyy 127213 _ 2 Click Next Prev Next gt d Step 3 physically remove the polymer bottle and replace with the care solution bottle Then click Next 1 650 872 0245 www mclab com 7 Change Polymer Wizard Step 3 Replace Polymer Notel Always wear appropriate protection when handling fluids used on this instrument Open th
8. Wash A0 MCLab E Drive For Wash SG i MCLeb E Drive For Wash mclabi CO 003 MCLob E Drive For Wash mclab11 Es MCLab E Drive For Wash MCLeb E Drive For Wash mclab11 603 1 MCLab E Drive For Wash 1 melab11 MCLab E Drive For Wash mclabit A04 d melab11 MCLeb E Drive For Wash a lt gt Description d Click Edit then Add Sample Run and set up a total of 10 cleaning runs Choose For Wash as the run protocol in the instrument protocol column Then click OK 14 4 amp MCLAB MA sequencingAnalysis Plate Editor Edit _ E well Results Group 10 Instrument Protocol 10 Analysis Protocol 10 AM MOLeb E Drive metab 801 Mash mclab1 1 MCLab E Drive New A me Mab EDrve pn mobil DO wah mclabit MCLab E Drive any dye promega mclabtt EM Mah mclabit MCLab E Drive o mclab11 FO mclab1 1 MCLab E Drive GA FHTRL moelab11 GU Longrun_cenemapper nci HO Wash mclab1 1 MCLab E Drive STORN mciabtt AD mci Mab EDive E mobil 802 Mah mclab11 MCLab E Drive For Wash mclab11 CO2 wah mclab1 1 MCLab E Drive For Wash mclab11 02 Mab E Dive Foch mobil 82 m MCLeb E Drive For
9. ay be difficult to remove with this procedure Any bubbles in this region will be removed later Wes Bubbles are present Ceme O No All bubbles are gone 2 Click Remove Bubbles Please wait approximately 1 minute for the Remove Bubbles procedure to complete 3 If bubbles are still present click the Remove Bubbles button again When all bubbles are gone click No All bubbles are gone Next gt 10 ES MCLAB Change Polymer Wizard Step 6 Remove Array Port Bubbles 1 Are bubbles visible in the array port around the array tip in the pump block carefully inspect the space above the array tip NOTE Bubbles less than about 0 2 mm in diameter may be ignored Flush array Port O No Al bubbles are gone 2 Loosen the array knob one turn counter clockwise Click Flush Array Port 3 Inspect the array port area for bubbles If bubbles are present make sure the array knob is loosened and repeat the flush procedure by clicking Flush Array Port 4 When the bubbles are gone tighten the array knob by turning it clockwise until it seats firmly in the pump block Click No All bubbles are gone mer J KEE zm g Step 7 Fill array with care solution Then click Finish 1 650 872 0245 www mclab com Change Polymer Wizard Step 7 Fill Array With Polymer 1 Ifyou would like to fill the array with polymer
10. e main door and remove the polymer bottle and supply tube cap from the instrument With a damp lint free lab wipe clean any liquid and dried polymer from the polymer supply tube and cap Dry these parts with another lab wipe tod Install a bottle of fresh polymer on the instrument Replace the shipping cap on the polymer bottle with the 1 hole supply tube cap Slip the polymer supply tube through the hole in the cap and mount the bottle in the holder Click Next i e Step 4 Click Prime to fill the fluid system with fresh care solution Then click Next 8 SB MCLAB Change Polymer Wizard Step 4 Prime the Pump with Fresh Polymer 1 Click Prime to fill the fluid system with fresh polymer Please wait approximately 1 minute for the Prime procedure to complete 2 Inspect the pump chamber the channels of both polymer blocks and all tubing for bubbles NOTE Removal of bubbles from all areas of the fluid system is important for problem free electrophoresis 3 Click Next Step 5 and Step 6 remove bubbles Finish 1 650 872 0245 www mclab com Change Polymer Wizard Step 5 Remove Bubbles 1 Are bubbles visible in the pump chamber the channels of either polymer block or any tubing NOTE Very small bubbles less than about 0 2 mm in diameter may be ignored Bubbles in restricted spaces of the array portin the pump block m
11. s 20 processen 2012 01 07 5457 Sema Neve 060612N perdia 0606120 0806120 Sesuencingvalsis perdina 2012 06 08 182120 807121 Dn Senueneincanansis serana 2012 06 07 191337 lt Cw Ca Cone Ce C Coon C Coma c The SequencingAnalysis Plate Editor will appear as the following screenshot 1 650 872 0245 www mclab com 13 Il sequencingAnalysis Plate Editor File 2 Fil Down Copy Paste oes Clear row s Shift Delete Fill Down Special 48 Cap At D Fil Down Special 96 Alt Shift D Resuts Group 10 instrument Protocol 10 Analysis Protocol10 Eum MCLab E Drive For Wash Bor melab11 MCLab E Drive For Wash co d MCLob E Drive For Wash melabi Dor d melab11 MCLab E Drive For Wash mclabit MCLab E Drive For Wash melabtt FU d melab11 MCLab E Drive For Wash cot MCLab E Drive For Wah Hort MCLab E Drive For Wash A02 melsbit MCLab E Drive For Wash B02 melab11 MCLab E Drive For Wash co 1 MCLab E Drive For Wash 002 1 MCLab E Drive For Wash melab11 melsbit MCLab E Drive For Wash 1 melab11 MCLeb E Drive For Wash mclab11 602 d melab11 MCLab E Drive For Wash 1 MCLab E Drive For
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