User Guide: HTS Customer Care Kits
Contents
1. the primary pump or in pump syringe or syringe needs replacement or pump syringes Symptom Possible Causes Solution See High carryover Wash station connections Replace wash station Chapter 3 are worn components Secondary Clog in sample coupler Replace sample coupler Chapter 5 pump Pump 2 tubing or in pump tubing or pump Chan error syringe or syringe needs syringes yi replacement Primary pump Clog in probe or tubing Replace probe tubing Chapter 4 Pump 1 error assembly from probe to assembly components Chapter 6 HTS Customer Care Kits User s Guide take up sample clogged assembly Symptom Possible Causes Solution See Wash station Wash station clog Replace the wash Chapter 3 overflows station assembly No events Clog in sample coupler Replace tubing Chapter 5 detected tubing or tubing assembly components assembly or sample coupler tubing Leak in sample Loose coupler or clog in Tighten coupler see Chapter 5 coupler coupler tubing precautions or replace sample coupler tubing when necessary Reduced sheath Sheath fluid filter is dirty Replace fluidic filter Chapter 7 flow or clogged every six months or when clogged Probe does not Probe might be bent or Replace probe tubing Chapter 8 About the HTS Illustrations of the HTS and a schematic of the fluidics system are provided for reference For more detailed information see2. 32 292 33 37 38 39 41 41 42 Overview of HTS Customer Care About the HTS Customer Care Kits The HTS Customer Care kits can help keep the BD High Throughput Sampler Option HTS in peak operating condition The HTS will experience problems if sheath fluid crystallization occurs in the connectors tubing and syringes Replacing these components every six months or annually along with daily cleaning and fluidics cleaning and shutdown can prevent clogs and optimize system performance The HTS Customer Care Kit offers a convenient way to replace clogged connectors tubing and syringes on site The kit which contains customer replaceable components and instructions reduces downtime and the need for depot repair 2 Kit Configurations The HTS Customer Care Kit is designed to custom fit the HTS configuration for each cytometer or group of cytometers Therefore order the correct kit number by using the following table Part number Description Cytometer 644787 HTS Kit Parts Replacement BD FACSCanto BD FACSCanto II 644788 HTS Kit Parts Replacement BD FACSCalibur BD LSR II including special order BD LSR II Special order BD LSRFortessa When to Use the Kit The parts in the HTS Customer Care Kit can be used to resolve common problems The following table is lists of symptoms possible causes and solutions that are described in this user s guide
3. Customer Care Kits User s Guide
4. accuracy BD Biosciences assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information BD Biosciences welcomes customer input on corrections and suggestions for improvement BD BD Logo and all other trademarks are property of Becton Dickinson and Company O 2010 BD FCC Information WARNING Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment NOTICE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his or her own expense Shielded cables must be used with this unit to ensure compliance with the Class A FCC limits This Class A digital apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Cet appareil num rique de la classe A respecte toutes les exigences
5. du R glement sur le mat riel brouilleur du Canada Compliance Information NOTICE This laboratory equipment has been tested and found to comply with the EMC and the Low Voltage Directives This includes FCC Part 15 compliance for a Class A Digital Device CAUTION Any unauthorized modifications to this laboratory equipment may affect the Regulatory Compliance items stated above History Revision Date Change Made 23 11165 00 Rev A 1 2010 Initial release Contents Chapter 1 Overview of HTS Customer Care 1 About the HTS Customer Care Kits 1 Kit Configurations ehh 2 When to Use the Kit s mr glee a chee whee og 2 About the PTS wate Kassa Br RE RA 3 HTS Configurations ehh 3 Location of Commonly Clogged Components 5 Safety and General Precautions 7 Biological Safety cuna ann en UR S 7 Electrical Safety ur 2a e E EU S wa ce UNA ead 7 AA ped acu du sx ess 7 Chapter 2 HTS Removal and Reinstallation 9 BD FACSCanto II System ccc ee ee ee hn 9 Removing the HT Sie seas sehen s eH 9 Reinstalling the HTS xk oec e RIPE a 11 BD FACSCanto BD LSR II BD FACSCalibur and BD LSRFortessa Systems 12 Removing we el EAR EPIS eb 12 Reinstalling the ATS ue ruin a weed 15 Chapter 3 Wash Station Assembly Replacement 17 Required Kit Com
6. your BD High Throughput Sampler User s Guide HTS Configurations The following figures show the front and rear views of the HTS for each cytometer Chapter 1 Overview of HTS Customer Care 3 4 Figure 1 1 HTS for the BD FACSCanto II system Figure 1 2 HTS for the BD FACSCalibur and BD FACSCanto systems Note The module body color for the BD FACSCanto is blue HTS Customer Care Kits User s Guide Figure 1 3 HTS for the BD LSR II special order BD LSR Il and BD LSRFortessa systems Note The module body color might be unique to your system NOTE The main HTS module shown is the same for all BD LSR products The BD LSRFortessa analyzer has an extra protective cover that mounts to the original BD LSR II HTS cover Location of Commonly Clogged Components The clogs that occur and need repair are in the fluidics system The following schematic identifies the most commonly clogged components The numbers refer to component parts in the Required Kit Components tables throughout this guide Chapter 1 Overview of HTS Customer Care 5 Figure 1 4 Schematic of the HTS fluidics system Flow cell 6 HTS Customer Care Kits User s Guide Safety and General Precautions Biological Safety A All instrument surfaces that come in contact with biological specimens EA can transmit potentially fatal disease Use universal safety precautions when changing tubing or removing part in contact wi
7. Chapter 7 Fluidic Connection and In line Fluidic Filter Replacement 39 THIS PAGE INTENTIONALLY LEFT BLANK 8 Probe and Probe Tubing Body Assembly Replacement The probe is located at the end of the probe assembly arm Replace the probe along with the probe tubing or body assembly annually or when the following occurs Probe is bent or is not taking up sample e Probe is clogged Required Kit Components Item number Part number Description Qty E 34389017 Probe tubing body assembly NOTE A spare probe is included in the HTS accessory kit Chapter 8 Probe and Probe Tubing Body Assembly Replacement 41 Replacing the Probe and Probe Tubing Body Assembly The sample injection port is fragile and can be easily damaged Use care when handling the sample injection port 1 Turn off the cytometer and remove the HTS cover NOTE On the BD FACSCanto BD LSR IL and BD FACS Calibur systems you will need to turn off the power on the cytometer and the HTS separately 2 Pull the HTS arm up slowly and move it toward the front of the instrument Probe tubing body assembly 42 HTS Customer Care Kits User s Guide 3 Unscrew the tubing connector from the probe Tubing connector Probe nut Probe 4 Unscrew the probe nut and remove the probe from the arm Probe Probe nut Tubing connector 5 Install and tighten a new probe a Tighten the probe nut at the top of the probe an
8. HTS Customer Care Kits User s Guide For Research Use Only Not for use in diagnostic or therapeutic procedures bdbiosciences com 23 11165 00 Rev A 1 2010 Becton Dickinson and Company BD Biosciences San Jose CA 95131 Tel 877 232 8995 Fax 800 325 9637 ResearchApplications bd com Asia Pacific Tel 65 6 861 0633 Fax 65 6 860 1593 Europe Tel 32 2 400 98 95 Fax 32 2 401 70 94 help biosciences europe bd com Brazil Tel 55 11 5185 9995 Fax 55 11 5185 9895 bdbiosciences brasil bd com Japan Nippon Becton Dickinson Company Ltd Toll Free 0120 8555 90 Tel 81 24 593 5405 Fax 81 24 593 5761 AY Canada Toll Free 888 259 0187 Tel 905 288 6000 Fax 888 229 9918 canada bd com Mexico Toll Free 01 800 236 2543 Tel 52 55 5999 8296 Fax 52 55 5999 8288 biocienciasmexico bd com O 2010 Becton Dickinson and Company All rights reserved No part of this publication may be reproduced transmitted transcribed stored in retrieval systems or translated into any language or computer language in any form or by any means electronic mechanical magnetic optical chemical manual or otherwise without prior written permission from BD Biosciences The information in this guide is subject to change without notice BD Biosciences reserves the right to change its products and services at any time to incorporate the latest technological developments Although this guide has been prepared with every precaution to ensure
9. Tighten the fitting until it contacts the sealing washer and then turn the fitting an additional 1 6 to 1 4 turn If leaking is observed tighten the fitting no more than an additional 1 8 turn Install the HTS on the cytometer see Chapter 2 Chapter 5 Sample Coupler and Tubing Replacement 29 BD FACSCalibur BD LSR Il or BD LSRFortessa Systems Item number Part number Description Qty Sa 335452 Sample coupler secondary pump to SIT 1 Sample coupler secondary pump to SIT Secondary pump 1 Fill a small clean container with approximately 300 mL of DI water 2 Remove the sheath line from the HTS 3 Connect the purge tubing to HTS sheath port see Chapter 9 30 HTS Customer Care Kits User s Guide 6 Pump Syringe Replacement Glass syringes are used in the primary and secondary pumps of the HTS unit For reference the syringe associated with the secondary pump is shown the following figure or Sharp raised edge Syringe Narrow up Syringe barrel 1 4 28 thread Perform the replacement of the pump syringe maintenance tasks annually or when the following symptoms occur e Primary or secondary pump Pump 1 or Pump 2 errors occur A Pump syringe has a clog NOTE It is not necessary to remove the HTS when replacing the primary pump syringe located on the front of the HTS However it is necessary to remove the HTS when replacing the secondary pum
10. are aligned to one another before screwing them together All fittings should be secured only finger tight and not beyond Connect the sample injection port to the sample injection port tubing assembly Connect the injection port tubing assembly to the sample injection tubing assembly Lower the sample injection port tubing assembly into the port hole and connect the silicon tubing to the sample injection port base as shown in the following figure The magnets will secure the sample injection port in place 20 HTS Customer Care Kits User s Guide 4 Install and tighten the sample injection tubing assembly to the top of the secondary pump and install the HTS on the cytometer see Chapter 2 Chapter 3 Wash Station Assembly Replacement 21 THIS PAGE INTENTIONALLY LEFT BLANK 4 Tubing Assembly Replacement Perform the tubing assembly from probe to the primary pump maintenance tasks when the following symptoms occur Tubing is clogged e The systems displays a primary pump Pump 1 error e No events are detected Required Kit Components Item m number Part Number Description Qty 4 335454 Tubing assembly sample probe to the 1 primary pump Chapter 4 Tubing Assembly Replacement 23 Clamp Tubing assembly Primary pump NOTE Use caution when handling the sample injection port assembly This assembly is fragile and can be easily damaged Replacing Tubing Assembly Compo
11. coupler Reinstalling the HTS 1 Place HTS on support bracket 2 Connect the interlock cable to the HTS 3 Connect the interface cable to the HTS 4 Connect the waste tubing to the waste connector on the HTS 5 Connect the sheath tubing to the sheath connector on the HTS 6 Connect the sampler coupler to the SIT on the cytometer Chapter 2 HTS Removal and Reinstallation 11 7 8 Tighten the captive screw that secures the HTS to the support bracket Turn on the cytometer BD FACSCanto BD LSR II BD FACSCalibur and BD LSRFortessa Systems 12 Removing the HTS 1 2 10 Turn off the cytometer and HTS Remove the HTS cover Unscrew the captive screw located at the bottom of the HTS support bracket NOTE There is no captive screw in BD LSRFortessa because the HTS is placed on a sliding drawer Pull the HTS out about 2 inches from the support bracket Remove the probe and the overflow reservoir Disconnect the power cable and the serial communications cable Disconnect the sampler coupler from the cytometer SIT Disconnect the sheath and waste connectors from the cytometer Remove the HTS from the support bracket a Support the HTS on its base raise the back of the HTS to balance the load and lift it off the support bracket Place the unit on a stable work beach and replace the necessary part HTS Customer Care Kits User s Guide Captive screw HTS positioned on its support bracket Chapt
12. d attach the tubing connector to the probe 6 Turn on the cytometer and HTS if needed 7 Start BD FACSDiva software and perform an HTS prime Chapter 8 Probe and Probe Tubing Body Assembly Replacement 3 THIS PAGE INTENTIONALLY LEFT BLANK 9 Cleaning Shutdown Procedure The BD FACSCalibur BD LSR IL and BD LSRFortessa do not have a Fluidic Shutdown cycle that automatically replaces BD FACSTM sheath solution with BD FACS shutdown solution or DI water These procedures are for the BD FACSCalibur BD LSR II Special Order BD LSR II and Special Order BD LSRFortessa systems as they do not have a fluidic shutdown cycle built into the software We recommend daily cleaning and maintenance of the sample coupler and tubing to avoid clogs Required Kit Components Item number Part number Description Qty 13 340088 HTS purge tubing 1 Cleaning Procedures 1 Fill a clean container with approximately 300 mL of deionized DI water 2 _ Remove the sheath line from the HTS 3 Connect the purge tubing to the HTS sheath port Chapter 9 Cleaning Shutdown Procedure 45 46 HTS purge tubing BD HTS sheath port Purge tubing DI water 4 Submerge the other end of the purge line into the DI water 5 Using BD FACSDiva software perform a system prime Repeat 3 times For BD FACS Calibur use BD Plate Manager software to run the prime 6 Remove the purge tubing 7 Reinstall the sheath line HTS
13. embly sample injection tubing to 1 HTS pump Chapter 3 Wash Station Assembly Replacement 17 Sampler coupler Sealing washer Injection port tubing Injection port wash station Replacing Wash Station Components 1 Perform the monthly cleaning procedure to decontaminate the sample injection port wash station For instructions see your BD High Throughput Sampler User s Guide Turn off the cytometer and HTS if needed Remove the HTS according to procedure in Chapter 2 Move the probe arm up and toward the front of the HTS Unscrew and remove the sample injection tubing from the top of the secondary pump 18 HTS Customer Care Kits User s Guide Tubing assembly Secondary pump The tubing nuts are normally finger tight If it is difficult to unscrew a tubing nut use a 5 16 wrench to loosen it Turn the sample injection port see figure slightly to disengage the magnets and lift the port approximately 1 inch Disconnect the silicon tubing from the sample injection port Do not disconnect the silicon tubing at the lower end because this could damage the lower connector Silicon tubing Chapter 3 Wash Station Assembly Replacement 19 Secure the silicon tubing so that it does not slide back into the hole and carefully remove the sample injection port assembly Assembling the Sample Injection Tubing Assembly A To prevent the cross threading of fittings ensure that mating fittings
14. er 2 HTS Removal and Reinstallation 13 Figure 2 1 Electrical connection to the HTS Power plug Serial cable 14 HTS Customer Care Kits User s Guide Figure 2 2 Fluidic connections to HTS Routing of power and communication cables Waste fluidic connection Sheath fluid connection Reinstalling the HTS 1 Place the HTS on the support bracket 2 Reconnect the power cable and the serial communications cable 3 Reconnect the sheath and waste connectors to the HTS Chapter 2 HTS Removal and Reinstallation 15 4 Slide the HTS unit as far as it can go 5 Secure the mounting screw to the bottom on the HTS 6 Place the cover back on the HTS and turn it on 7 Attach the sample coupler to the SIT 8 Turn on the cytometer 9 Start BD FACSDiva software or Plate manager software if connected to the BD FACS Calibur and perform an HTS prime 16 HTS Customer Care Kits User s Guide 3 Wash Station Assembly Replacement Perform the wash station assembly maintenance tasks every six months or when the following symptoms occur The HTS exhibits higher than normal carryover between samples e The wash station overflows Required Kit Components The sample injection tubing 3 connects the injection port wash station 1 to the secondary pump valve not shown Item Part Description Qty number Number 1 336947 Injection port wash station 1 2 335526 Tubing injection port 1 3 335451 Tubing ass
15. he new syringe a Screw the syringe barrel onto the valve until it contacts the seal washer then turn the syringe an additional 1 6 to 1 4 turn If you observe a leak tighten a maximum of an additional 1 8 turn b Pull the syringe plunger down to the plunger holder see the following figure Chapter 6 Pump Syringe Replacement 33 Tighten the plunger lock screw making sure it is securely tightened P d we Pl ee Lubricant Plunger holder 6 Apply a generous amount of O ring lubricant to the plunger lock screw to prevent seizing in the future You can apply the lubricant in the lock lever hole before placing the plunger in it This will help keep the saline out of the whole assembly Do not lubricate the syringe barrel 7 For the secondary syringe reinstall the HTS on the cytometer see Chapter 2 34 HTS Customer Care Kits User s Guide 8 Turn on the cytometer and using the BD FACSDiva software perform a Prime Lg Secondary pump Syringe Chapter 6 Pump Syringe Replacement 35 THIS PAGE INTENTIONALLY LEFT BLANK 7 Fluidic Connection and In line Fluidic Filter Replacement This chapter covers the replacement of the two fluidic connections between the cytometer and the HTS These two fluidic connections are sheath and waste connections Perform the fluidic connections maintenance and replace the sheath filter e Every six months e When the sheath flow is reduced or inadequate Chapter 7 Flu
16. idic Connection and In line Fluidic Filter Replacement 37 Required Kit Components Item number Part number Description Qty 8 335710 Filter 25 mm 17 pm screen 1 9 343527 Coupling insert hose barb blue 1 10 59 10090 05 Coupling insert hose barb orange 1 waste 11 343529 Coupling insert hose barb white 1 Figure 7 1 Filter Filter Figure 7 2 BD FACSCanto Il connectors Blue hose barb Orange hose barb Filter White hose barb 38 HTS Customer Care Kits User s Guide Replacing the Filters 1 Turn off and remove the HTS from the cytometer see Chapter 2 2 Replace the two liquid connections between the cytometer and the HTS a Move the HTS away from the cytometer to provide access behind the HTS Disconnect the quick connector at the cytometer interface panel and the back of the HTS orange quick connector end in Canto II Disconnect the quick connector at the cytometer interface panel and at the white quick connector on the back of the HTS unit blue quick connector end in Canto IT Remove the tubing from the filter by twisting the Luer connections Install the sheath line with the lock ring end toward the cytometer panel This will prevent foreign material that accumulates on one side of the filter from flushing into the HTS if the filter is reversed 3 Replace the filter and reconnect all tubing 4 Install the HTS on the cytometer see Chapter 2
17. nents 1 Turn off the cytometer and HTS and move the sample probe into the sample injection port 2 Remove the sample probe tubing assembly 3 Unscrew the sample injection tubing from the probe and the top of primary pump The sample injection tubing connectors are indicated in the following figure 24 HTS Customer Care Kits User s Guide Remove the sample probe tubing by rotating the tubing clamp on the arm to allow enough clearance for the tubing to be removed If a connector is difficult to remove due to saline deposits clean it with DI water If it still does not unscrew then carefully use the 5 16 wrench and gently loosen the clamp Do not force Connect the sample probe tubing NOTE Valves contain sealing washers in each port If you over tighten the fitting the sealing washers can be compressed resulting in a blocked port Tighten the fitting until it contacts the sealing washer and then turn the fitting an additional 1 6 to 1 4 turn If leaking is observed tighten the fitting no more than an additional 1 8 turn Install the sample probe tubing in the clamp on the arm and rotate the clamp back into position Install and tighten the sample injection tubing to the sample probe and to the top of the primary pump Turn on the cytometer and perform a Prime Chapter 4 Tubing Assembly Replacement 25 THIS PAGE INTENTIONALLY LEFT BLANK gt Sample Coupler and Tubing Replacement The sample coupler is freque
18. ntly installed or removed from the SIT to run the cytometer in tube acquisition mode The sample coupler tubing is attached to the HTS secondary pump Replace the sample coupler tubing annually or when the following symptoms occur e The system displays a secondary pump Pump 2 error The sample coupler continues to leak after being tightened A rare symptom can be no events are detected Chapter 5 Sample Coupler and Tubing Replacement 27 BD FACSCanto and FACSCanto II Systems Item number Part number Description Qty 3 339340 Sample coupler secondary pump to SIT Figure 5 1 Sample coupler and tubing for the BD FACSCanto and BD FACSCanto II systems Secondary pump Sample coupler secondary pump to SIT 1 Turn off the cytometer and remove the HTS from the cytometer see Chapter 2 2 Unscrew the sample coupler tubing from the left port of secondary pump valve indicated in the figure and discard the sample coupler with tubing into a biohazardous waste container 28 HTS Customer Care Kits User s Guide Secondary pump valve Install the new sample coupler tubing provided in the HTS Customer Care Kit Connect the new tubing to the left port in the secondary pump valve and tighten the tubing until the fitting is finger tight NOTE Valves contain sealing washers in each port If you over tighten the fitting the sealing washers can be compressed resulting in a blocked port
19. o disconnect the waste and sheath fittings from the HTS All instrument surfaces that come in contact with biological specimens A can transmit potentially fatal disease BD FACSCanto II System Removing the HTS 1 Turn off the cytometer and HTS 2 Remove or open the BD High Throughput Sampler safety cover Chapter 2 HTS Removal and Reinstallation 9 3 Detach the sample coupler from the cytometer SIT a Unscrew the top nut b Pull the sample coupler down and away from the SIT leaving the nut attached to the sample coupler 4 Unscrew the captive screw located at the bottom of the HTS support bracket 5 Pull the HTS out about 2 inches from the support bracket 6 Remove the probe and the overflow reservoir 7 Disconnect interlock and interface cables 8 Disconnect the sheath and waste connectors from the HTS 9 Disconnect the sampler coupler from the cytometer SIT 10 Disconnect the door sensor cable a Lift the front feet of the HTS just over the front edge of the enclosure and tilt the unit at a 45 angle toward you for access to the door sensor cable on the right side b Unplug the door sensor cable by pulling back on the ferrule Place the cable over the right side of the enclosure carrier 10 HTS Customer Care Kits User s Guide 11 Place the HTS unit on a stable work bench Sheath tubing connection Waste tubing Interlock cable connection connection Interface cable connection Sampler
20. p syringe located on the rear of the HTS Chapter 6 Pump Syringe Replacement 31 Required Kit Components Item number Part number Description Qty 6 344912 Syringe 500 pL with black plunger seal 1 y 347306 Lubricant O ring Super O Lube 1 ounce tube Pump Syringe Replacement Procedures e If you are replacing the primary pump use both procedures in this chapter e If you are replacing the secondary pump remove the HTS see Chapter 2 then use the Replacing the Syringe procedures Removing Liquid from the Syringe For Primary Pump Syringe 1 Verify that the cytometer and BD FACSDiva software are running 2 From the Acquisition Dashboard select RUN PLATE 3 Remove the liquid from the syringe a Disconnect the sheath connector from the back of the HTS b Disconnect one end of the sheath filter c Select HTS gt Prime d Repeat until the syringe barrel is empty e Exit BD FACSDiva software 4 Turn off the cytometer 32 HTS Customer Care Kits User s Guide Replacing the Syringe 1 Loosen the plunger lock screw indicated in the following figure approximately three full turns Plunger lock screw 2 Lower the plunger drive by pushing the plunger lock screw down 3 Unscrew the syringe barrel and remove it from the lower part of the valve Grasp the metal ring at the top of the syringe and turn counterclockwise 4 Discard the used syringe into a biohazard sharps container 5 Install t
21. ponents 17 Replacing Wash Station 18 Assembling the Sample Injection Tubing Assembly 20 Chapter 4 Tubing Assembly Replacement Required Kit Components Replacing Tubing Assembly Components Chapter 5 Sample Coupler and Tubing Replacement BD FACSCanto and FACSCanto Systems BD FACSCalibur BD LSR II or BD LSRFortessa Systems Chapter 6 Pump Syringe Replacement Required Kit Components Pump Syringe Replacement Removing Liquid from the Syringe For Primary Pump Syringe Replacing the Syringe Chapter 7 Fluidic Connection and In line Fluidic Filter Replacement Required Kit Components Replacing the Filters 2 222 220 a a Chapter 8 Probe and Probe Tubing Body Assembly Replacement Required Kit Components Replacing the Probe and Probe Tubing Body Assembly Chapter 9 Cleaning Shutdown Procedure Required Kit Components Cleaning Procedures vi HTS Customer Care Kits User s Guide 23 23 24 27 28 30 31 32
22. th the fluidics system Wear suitable protective clothing eyewear and gloves Electrical Safety Ensure that the power to the HTS is turned off before handling electrical cables and their connections Precautions 1 Tighten all fittings finger tight Do not use tools to further tighten connectors 2 Ifa connector is difficult to remove because of saline deposits clean with deionized DI water then carefully use the 5 16 wrench in the accessory kit to remove the connector 3 The sealing washers in the valves of each port can be compressed and will eventually block the port To avoid compression tighten fittings until they make contact with the sealing washer and then turn the fitting an additional 1 6 to 1 4 turn If there is a leak tighten the fitting no more than an additional 1 8 turn Chapter 1 Overview of HTS Customer Care 7 THIS PAGE INTENTIONALLY LEFT BLANK 2 HTS Removal and Reinstallation Before performing replacement procedures remove the HTS from the cytometer Pay particular attention to the following e Always disconnect electrical connections interlock cable and interface cable and fluidic connections waste sheath and sample injection tubing SIT The waste and sheath fittings on the back side of the HTS can easily be broken when moving the HTS away from the cytometer while these fittings are connected Move the HTS just enough approximately 2 inches to provide the room necessary t
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