ToxiVialsT'Vl type A & B User Guide
Contents
1. X fo y 1 gt JA A INTERFINE CHEMICALS ANALYTICAL SCIENCES BIOCHROMATOGRAPHY LIFE SCIENCES 33 4 70 03 73 01 interfine interchim fr www interchim com ITM 2013 09 23 C P ToxiVials type A amp B User Guide Step 1 Sample introduction Step 2 Mixing ToxiVials type A Add 5ml of sample w internal standard ToxiVials type B Add 4 5ml of sample w internal standard Shake manually for 2 to 5 minutes Internal standards are not mandatory Step 3 Centrifugation Centrifuge at high speed 2500 rpm for 5 minutes for the extraction of neutral amp basic compounds ToxiVials type B are recommended for the extraction of neutral amp acidic compound Step 4 Supernatant treatment Step 5 Reconstitution Transfer the organic supernatant into appropriate concentration cups then evaporate to dryness Reconstitute using the appropriate solvent and smallest volume and get ready for the analysis Results can be different depending on the analysis technique used following the ToxiVials extraction ANALYTICAL SCIENCES 2 m 211 bis avenue JF Kennedy interc h M 22122 22100 montisn Hotline 33 4 70 03 73 09 Be interchrom interchim com
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