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iEMS Incubator Shaker - User Manual

1. Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 15062004 Thermo Scientific EMS Incubator Shaker Cat no 5112200 and 5112207 User Manual Rev 3 4 June 2009 Cat no 1506200A Copyright Copyright O 2009 Thermo Fisher Scientific Corporation All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks 8 and EMS and Microtiter are trademarks of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Contact information Thermo Fisher Scientific Oy P O Box 100 Fl O1621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com
2. Fig 7 1 Opening the voltage selector fuse block assembly 3 Replace the blown fuses with the same type 3 15 A See Fig 7 2 1 Fuses 2 Voltage selector Nee A y 7 Fig 7 2 Fuse block assembly 4 Place the fuse holder back into the slot and push it in until you hear a click 5 Connect the power cable to the instrument and switch it ON Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 35 Chapter 7 Maintenance 7 3 Disposal of materials Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used microplates disposable gloves syringes disposable tips etc as biohazardous waste 7 4 Decontamination procedure A A A A Caution Only use a cloth dampened with a decontaminant to decontaminate the instrument The use of spraying may damage the instrument Warning To prevent electrical shock do not reach into a slot and or touch the power contacts at the rear of the slot when the power is turned on to the instrument If you need to access the slot for example for cleaning turn the power off to the instrument and unplug the power cable Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed A decontamination procedure is only recommendable when infectious substances ha
3. Chapter 6 Operation Program the incubation time by dialing the number keys first hours then minutes and finally seconds o Note Check where the cursor underline is located before you enter a value I1 The value will be entered at the location of the cursor For example for an incubation time of 1 hour 30 minutes Press 01 30 00 INC TIME 01 30 00 SHAKE TIME 00 00 00 n Note If you want to interrupt the programming press Stop to return to the 1 READY state Your changes will be saved Program the shaking time by dialing the number keys first seconds then hours and finally minutes It is recommended that the same shaking time is used in all slots in one module If you need different shaking times use slots in different modules o Note Check where the cursor underline is located before you enter a value 1 The value will be entered at the location of the cursor For example for a shaking time of 20 seconds SHAKE TIME 00 00 20 Press 00 00 20 INT TIME 00 00 00 Note If you want to interrupt programming press StOp to return to the 1 READY state Your changes will be saved Interval time is a programmable parameter that allows you to specify at what interval the shaking is alternately switched off and on for example an interval time of 00 00 10 results in 10 seconds of shaking turned on 10 seconds of shaking turned off 10 seconds of shaking turned on etc for the duration of the programmed shaking
4. Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 2 Table of Contents Table of Contents O oo N OO Bb Y DY 2 gt 2 N 0 13 Appendix A Appendix B Appendix C Appendix D Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A Safety Symbols and MarkingS oocoonncccccnoncccconononccnonnnnncnnnnnnnnnnnnnnnnnnnannnnnrnnnnnnnrnnaannrs 7 About the User Manal oai iicisccccccsteccccctecccccsetsscceccctececesteccecssetecesestneee cine 8 Introduction to the EMSTM Incubator Shaker ccccccesceeseeeeeeeeeeeeeeeseeeeeseeeeeeneees 9 Functional Description ccccceeeeeeeeeeeeeeeeeeeeeeeaeeeeeee sees eaaeaeeeeeeeseeeaaeeesaeeeeeseeeeaes 10 Installation jcccccsicccccsseccceesescceedeccteecevesencdeesecdeetecctecevscdeceeesdccceevssscdeevsscteecuvsbecceuvsscceeeyasces 14 Operativo aia ad oi A AEE EEA ONANAN AAAS 18 MAINTENANCE ec 34 Troubleshooting Guide cc iii dina 39 Technical SpecificatiONS ooocoonnocccnnnocncnnnnonnnnnnnonnnononnannnnnnnnnnnrnnnnnn nr rnnn nn nr rn anar rr rra 44 Ordering InformatioN occccinnocr diiniita ianiad atadas 48 Warranty Certificate iii ii ee he 49 AAA me A A 50 Glossary and Abbreviation iicc cccceisceccessscccevesssccdeeessdeveesssscevaessccveveesscaveessssceeaesace 53 Thermo Scientific EMS Incubator Shaker Quick Reference Guide 54 Certificate of Decontamin
5. NCCLS Document 117 P Vol 11 No 15 Villanova Pa NCCLS 1991 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 37 Chapter 7 Maintenance 7 5 How to pack for service When you ship the instrument for service remember to e Inform about the use of hazardous materials e Decontaminate the instrument beforehand e Pack the instrument according to the enclosed packing instructions e Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional labor charges e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department See Section 9 1 for details on storage and transportation temperatures See also Section 8 3 Service request protocol 7 6 Disposal of the instrument Warning Decontaminate the instrument prior to disposal See Section 7 4 and Appendix B on decontamination Follow laboratory and country specific procedures for biohazardous or radioactive waste dispoal Warning The used batteries are regulated waste and must be dispose
6. Shaker User Manual Rev 3 4 Cat no 1506200A 40 Chapter 8 Troubleshooting Guide Do not reach into slot when power is turned on gt To prevent electric shock do not reach into a slot and or touch the power contacts at the rear of the slot when the power is turned on to the instrument If you need to access the slot for example for cleaning turn the power off to the instrument and unplug the power cable High temperatures gt The thermal microplate holders are designed to stop heating if temperatures reach 53 C 10 C in the unlikely event that temperature control to a holder is lost or enters a runaway condition Microplate holders at this temperature will be hot when touched To help prevent loss of temperature control ensure the power contacts on the back of the microplate holder are clean and free from dirt or corrosion and the holder is correctly inserted into the slot before you start the processing protocol 823 Temperature Changing programmed temperature setting When you change the programmed temperature setting make sure the slot temperature is at the new target setting before you start the processing protocol The instrument does not prevent the processing protocol from starting if the slot is not at the target temperature You can view the actual slot temperatures on the instrument display Refer to Section 6 5 Slot warmup time Allow the slots to reach the programmed target temperature before you star
7. that the holder is correctly inserted into the slot before you start the processing protocol A Warning The thermal microplate holders are designed to stop heating if Instrument reset due to power loss If the power to the instrument is lost the instrument will reset to the READY mode when power is restored All shaking and incubation functions including timing will be turned off No LEDs will be lit and the current time of day will be shown on the display 6 5 Preheating and heating Operation Display Press temp followed by the slot number to display the temperature in a particular slot or to start the preheating of the thermal microplate holders PLACE NUMBER Press temp Press the slot number 1 9 For example Press 1 PLACE 1 SET 30 0 C HEAT OFF TRUE 25 7 C The actual TRUE slot temperature o and heating status HEAT OFF or HEAT PEACE SET 30 0 C ON are displayed HEAT ON TRUE 37 0 C If the thermal microplate holder is not PLACE 1 SET 30 0 C installed or inserted correctly the display will show HEATON TRUE C Press start to turn the heating on Press stop to turn the heating off Press a number key or J Enter to move to another slot Before the temperature has reached the set value the LED flashes with an orange color After the temperature has reached the set value the orange LED lights up continuously Press temp 0 to display the heating status of all slots If the heating is ON in ever
8. 1150 1400 Caution Splashing may occur if you use shaking with wells that contain too much liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3 in most cases Program the shaking speed by pressing the appropriate number key SHAKE SPEED O rpm corresponding to the required speed VALUE 012345 For example for a speed of 900 rpm SHAKE SPEED 900 rpm VALUE 012345 Press 3 Enter If no shaking is desired select the value 0 In this incubation module shaking is now disabled in every slot If you try to start a slot with a protocol PLACE 2 with shaking an error message will PROHIBITED OPERATION appear on the display On the display the READY state will 09 26 15 appear after the programming phase ere Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 21 Chapter 6 Operation 6 3 Slot status control LEDs and audio signal Each slot of the iEMS Incubator Shaker has a control LED light emitting diode that displays the status of the slot The LED will change to a specific color based on the status of the slot An audio signal is given when the green LED is turned on see Section 6 4 3 6 The colors of the LED are defined in the table below
9. 27 6 4 3 6 Turning off the audio signal asses T EE EET E 27 6 4 4 Removing the microplate ccccceeeeseeseeeeeeeeeeeeeeneaaeaeee seen nnnnn rre rra 28 6 5 Preheating and heating xs ccc morir dr ide A ta di ai an AEA 30 6 6 Saki cid ii a aii 32 6 7 Verifying incubation and shaking parameters cccconocccccnononcnnnanoncnnnnna cnn nn nano nnnnnnnannnnnnns 33 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 4 7 1 7 1 1 7 1 2 7 2 7 3 7 4 7 4 1 7 4 2 7 5 7 6 8 1 8 2 8 2 1 8 2 2 8 2 3 8 2 4 8 2 5 8 2 6 8 3 9 9 1 9 2 9 3 9 4 9 5 10 10 1 11 11 1 12 13 13 1 Appendix A Appendix B Appendix C Appendix D Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A Table of Contents A ccd bsuhesnactcenccuadanncedehccdceuadhncedsacess cussvandvancdntecedsadtacese 34 Regular and preventive maintenance occcccccccnnconnnoncnnnnnnnnnnnnnn cnn nnnnn nana nr rr nr rre 34 Ce A vetoes sees Eien AA E PE tiered EE E naa fave EE E 34 immediates a ci a e a aa daaa te a added 34 How to replace FUSES etenee ien aaa dal Aa E LEANE KEEA eea R 35 Disposal of materialS serioa aE EEE E E dh 36 Decontamination procedure cococcccccnnccononononcnnnnnnnnnnnn conc cnn nn nena rn rre 36 How to decontaminate the instrument SUITACO oooocccccnononcnnnaninnnnnanonnnnnnnannnnnnnnnnnnnnnns 37 How to decontaminate the thermal microplate holder ooonnnnin
10. F Feedback For Micra n E E E EEEE EA E liti A 56 FUSE block assembly esiin ananin anaa aiaia la aaa aaa A dit 16 A NN 35 44 H A A aa aaua a NA 30 FAS ATI GUTTA Saag A ias 20 Holder o ii id 13 Incubation temperature A A deudcenasc us vaglsadbudenasvasuacsuancwedte 19 incubation CANE iia A e o i 20 Incubation TIME trackING cxioconits tin A e a a Ed A AR e 40 Incubator Slot prelneatInd siii a ci di 25 39 Inserting THE microplate miii ae da cies 23 Installation ca 14 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 50 Chapter 12 Index CALOGORY A OT 46 check ee Re ee edn Res ee ling E 14 SOULS ira A A eee EE AAA A ADA AA AA A dhe duees 16 Mila o nte a io In ia ddr 15 VEN HUI CA a a sede e rn adi 9 advantages ii Sie ead i let ee Re ee 9 CLEANING iia iaa 34 decontamination A dd a 36 A A AO 38 INS PEC ir ed add 15 MAIN a tit a aaa 47 packing for Servi iia E A ct tas 38 A O 14 E AO TE 9 Interval iMac ii ias 20 K KO YD hel atta deed aa alea edie adh Apel E E Pete dans hotel che pe ens pete teceeds 12 CLEANING ta At A ans ii 34 Keyboard KEYS A A A eae 12 L LC GIS Dla ies huida dada idad dada data 12 M Maihs supplyCablE cin a ed 17 MAINS SWITCH 0 it ii tt ai 17 MaintemanCe 2 aaa aea aaa ete ee eee 34 Microplate USOS di A A A A E AA aise 36 42 E NN 23 Microplate holder cta A ia 13 O Operating CONGITIONS iii iia 15 Operation Noain a dad 26 27 preheating iandih atind trad 30 A
11. Inaccuracy 0 3 C calculated as the mean of the minimum and maximum well temperatures of wells A1 A12 H1 H12 and E7 after 1 hour at 37 C with an ambient temperature of 24 C with 200 ul water well without a plate sealer and without shaking Uniformity lt 0 3 C across the entire plate calculated as the difference between the maximum and minimum well temperatures of wells A1 A12 H1 H12 and E7 after 1 hour at 37 C with an ambient temperature of 24 C with 200 ul water well without a plate sealer and without shaking Evaporation lt 300 mg plate after 1 hour at 37 C without plate sealer 9 3 Shaker specifications Performance of the incubator with flat bottomed microplates with nominal voltages Frequency 400 1400 revolutions per minute rpm in steps of 250 rpm Speed settings 0 0 rpm 1 400 rpm 2 650 rpm 3 900 rpm 4 1150 rpm 5 1400 rpm Tolerance 50 rpm Diameter 1 mm radius 0 5 mm Programmable shaking time Up to 48 hours in steps of 1 second Programmable interval time Up to 48 hours in steps of 1 second Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 45 Chapter 10 Device Interfaces 9 4 Safety specifications The iEMS Incubator Shaker fulfills the following requirements EN 61010 1 2001 Ed 2 and including CA US National Differences EN 61010 2 010 2003 Ed 2 The safety specifications are
12. Section 6 3 Slot status control LEDs and audio signal for more information The LED turns to a continuous orange color when the slot reaches the target temperature However the continuous orange LED may falsely indicate that a slot is at the target temperature under the following conditions e If you remove the thermal microplate holder and then reinsert it into the slot during the initial preheating of the slot e If the programmed target temperature is lower than the actual temperature of the slot Incubator slot preheating When you power on the instrument heating of the incubator slots does not occur automatically You must turn the temperature function on Preheating after you have powered on the instrument Refer to Section 6 5 Preheating and heating Module incubation temperature All slots within a module will be heated to the same temperature The slots within a module are kept at the temperature that is programmed into the first started processing protocol in the module If another temperature is programmed to another slot in the same module the program cannot be started The message PROHIBITED OPERATION will display Refer to Section 6 4 3 Operation Module shake speed All slots within a module will shake at the same speed The slots within a module shake at the speed that is programmed into the most recently started processing protocol Refer to Section 8 2 4 Shaking Do not place the microplate holder in or remove
13. Table 6 2 LED color codes Color code Explanation Flashing orange O The heating has been turned on but the set temperature has not yet been reached Orange The slot has reached the set temperature and is ready for operation The slot is in use shaking and or incubating Green The processing protocol is complete and the heating of the slot is still on The orange control LED flashes when a slot is heating up to the programmed target temperature The LED turns to a continuous orange color when the slot reaches the target temperature However the continuous orange LED may falsely indicate that a slot is at the target temperature under the following conditions e f you remove the thermal microplate holder and then reinsert it into the slot during the initial preheating of the slot e lf the programmed target temperature is lower than the actual temperature of the slot Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 22 6 4 Chapter 6 Operation Normal operation A A A A 6 41 Placing the microplate Caution To minimize the possibility of splashing aerosols and evaporation during shaking and incubation it is recommended that you use microplate sealers Open the cover of the thermal microplate holder and place the microplate into the holder so that well A1 is located in the upper left corner as shown in Fig 6 2 Caution Do no
14. also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude up to 2000 m Temperature 5 C 40 C Mains supply fluctuations 10 from nominal Installation category ll according to IEC 60664 1 see Note 1 overvoltage category Pollution degree 2 according to IEC 60664 1 see Note 2 o Note 1 The installation category overvoltage category defines the level of 1 transient overvoltage which the instrument is designed to withstand safely It depends on the nature of the electricity supply and its means of overvoltage protection For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply o Note 2 The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Both of these affect the dimensioning of the electrical insulation within the instrument Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 46 Chapter 10 Device Interfaces 9 5 In conformity with the re
15. connectors at the rear of the slot Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 24 Chapter 6 Operation 6 4 2 Before pressing start Ensure that the thermal microplate holder is latched into its normal operating position Ensure that you have inserted the thermal microplate holder correctly into the slot before you start the processing protocol When inserting a microplate holder into a slot push the holder completely in and down to latch it into its normal operating position see Fig 6 3 and Fig 6 4 If the microplate holder is not latched into position it may lose contact with the temperature controlling connectors at the rear of the slot while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism Incubator slot preheating When you power on the instrument heating of the incubator slots does not occur automatically You must turn the temperature function on see Section 6 5 Preheating and heating after you have powered on the instrument Allow the slots to reach the programmed target temperature before you start any processing protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange Before you start the processing protocol ensure that the temperature of the slot is within the acceptable range appropriate for the application
16. it from the instrument while the instrument is shaking This may damage the shaking mechanism 8 2 Warnings and cautions 8 2 1 Installation When unpacking the instrument it is recommended that two people lift the instrument together taking proper precautions to avoid injury Do not touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 39 Chapter 8 Troubleshooting Guide Do not operate the instrument in an environment where potentially damaging liquids or gases are present Never operate your instrument from a power outlet that has no ground connection Never use a mains supply cable other than the mains supply cable designed for your region 8 2 2 General Thermal microplate holders Do not handle the thermal microplate holder from its cover since it will come off The covers of the thermal microplate holders are not spring loaded but you should take care not to trap your fingers when closing the cover of the holder Avoid bumping the microplate holder while inserting it into the slot Viewing programmed processing parameters You can only view programmed processing parameters while in the programming mode When you review the parameters make sure that you do not unintentionally change the programmed values Refer to Sec
17. nano nn rra nr rr 15 5 2 3 Checking for damage during transport nnccccnnnicccnnnnocccnnano cnn nnano rn rnnnnr rr rra rra 15 5 2 4 Climatic requirements iii dada 15 5 2 5 TRINOS LO AMO lis ta nata 15 5 2 6 Technical prerequisite cria a O Abs cobb ie air aeia 16 5 3 Setups before you put the instrument into OperatiON ooocccconcccncnancnnnnnonananncnnnnnnnncnnnos 16 5 3 1 Voltage Selector ia di 16 5 3 2 Mains Supply Gable iia a tn 17 5 3 3 SOLU O E EE E A A A RE A A EE AET 17 6 A a EERE EEEE E ET E E T TT 18 6 1 Principle Of OperatiON onnccconnnoccccnnononnnnnononnn nan nn e EEEREN A rra 18 6 2 Programming Protocol Simnar ad a rs 19 6 3 Slot status control LEDs and audio SigNal ooooccnnnocccccnonoccncnanincnnnnnnnnnnnnnnonnnnnnarannnnnnos 22 6 4 Normal OperatiO cod ra E A A A E 23 6 4 1 Placing the Microplate kiise inean bo 23 6 4 2 Before pressing Start eorias rnn Ea ERE A AAEE E a RENEA EEEE E EES 25 6 4 3 OPE FALL ON sea rea A R E te EE AOS 26 6 4 3 1 Starting the OPESrati ON e eeoa Ere RE Ea EE Pe A E EA RE ENNEA EE EFENA REKENAARS EEN 26 6 4 3 2 Checking the Operation time ooccccnnnoccccnnonocncnnanoncnn nano n nn nro cnn 26 6 4 3 3 Starting a program during operation in another SlOt ooconnnccnnnnicccnnnnccncnnanarrncnnann cnn 26 6 4 3 4 Checking the Operation stop tiMB oonnccccnnnocccnnononnnnnononnnnnnrnnnn cnn ron nn nn rra nn rn r narran 27 6 4 3 5 Stopping the operatios serr eienen R ENa AE cnn REKE AI RE ENANA rre
18. not included in the warranty Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 49 Chapter 12 Index A ACCESS OMGS asione ie a a a E 48 INS POCO isis aaaea iaa a Ea aaa aieea aaa aiaa a A ea TARA AA DEA li 15 unpatkiN gissen aa a A A A AA 14 Aerosol A a O O AN 40 Application A diia 56 AUdiO SI lists ceedeensieatences cue deciass ii ta abia ida 22 TUNING Oh EE E a a T E A das T 27 C Catal OG MUMDG lS vecina A A ias 2 Certificate of DECONLAMINA ION iii A A a 55 CHECK A AO 14 Glock Meu A ta 17 Complaint Ord OM caia iaa 43 Consumables warranty MIMI tatl ONS sinian ia A aa Aed aa a ETE aaaeaii aeaa 49 COMmtaCt informatio asies A a ea 57 Control LED Snina aaa ea ias 10 22 39 D DECONTAMINATION vive ccecceseccacecascvecsvarescusves odeceeesstiavesstceaneestcesceresdvaasssetssueasacseucssucsederesdversveedsarerctacetes 36 38 Certificate diia ia SER 55 instrument SUMA COL cocaina ei a e e declarada 37 POCO UE A A EN 36 thermal microplate holder ooconnncnccccnnnnnnanonononncnnnnn cnn eee nea cnn nn nene 37 DIMENSIONS ci ad ens 44 Directive S Sui titanio 47 DisinfectaniSiaci iia ia daa aaa tas 36 Display Sea anaa aA AAA ARA A AE AS 12 Disposal MVS EDIE N see oe ch at ences aee eena a aa e ea e a ea 38 materials icon a n a 36 E Environmental CONdItiONS cccccccccccccnnnncncnononononononononononononn nono nn nn nn nn nono nn nono AA aE DAA ENAA E AE A EARE 44 Evap rati M aiii A A A A A Re 40 45
19. press start The shaking time and speed are the same as the parameters set in the programming phase After pressing shake you can select the slot numbers by pressing number keys or by scrolling with the Enter key Within a module if a slot has a protocol in progress with a shaking PLACE 2 speed of 0 another protocol with a PROHIBITED OPERATION nonzero shaking speed will not start and the display will show After the shaking protocol has finished the control LED turns to green and 09 29 35 you will hear a beeping sound To stop shaking before the shaking time has elapsed Press the slot number and the stop key The protocol stops the LED turns to green and you will hear a beeping sound Press C to return to the READY state if 09 29 15 you do not need to start a shake Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 32 Chapter 6 Operation 6 7 Verifying incubation and shaking parameters You can display the parameters programmed for each slot To display the programmed parameters press the appropriate slot number and then press Enter Continue to press J to display the programmed parameters for temperature incubation time shake time interval time and shake speed slot while the slot is not heating or shaking When you review the parameters ensure that you do not make any unintentional changes to the programmed values A Caution You can only view programmed incubat
20. single protocol for all slots or an individual protocol for each slot The parameters are stored in the instrument memory until you enter new parameters You can change the parameters of one slot without affecting the parameters of the other slots In one module all slots have the same incubation temperature and they shake at the same speed You should preheat the slots to the programmed target temperature before you start any protocol see Section 6 5 Preheating and heating Operation Display 09 25 15 Switch the power ON Note If the power is already on any shaking or heating must be turned off in the slot to be able to program a protocol To program the incubation and shaking parameters press the appropriate slot number 1 9 For example PLACE 1 09 25 15 Press 1 Enter TEMPERATURE 37 0 C If you want to program the same parameters to all slots PLACES 1 9 Press 0 J TEMPERATURE 37 0 C Program the new temperature value by dialing the number keys or press 4 Enter to accept the existing one Note Check where the cursor underline is located before you enter a value The value will be entered at the location of the cursor For example TEMPERATURE 30 0 C Press 30 INC TIME 00 00 00 Note If you want to interrupt the programming press Stop to return to the READY state Your changes will be saved Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 19
21. still on If you wish to turn the heating off press the slot number 1 9 and then press stop Note Do not remove the thermal microplate holder until the incubation time has elapsed If you remove the microplate holder from the slot before the incubation time has elapsed the incubation timer does not stop and continues to count down causing tracking of actual incubation time to be lost 2 After the shaking and incubation have been completed you can remove the thermal microplate holder lift the handle upwards Fig 6 5 and pull out Fig 6 6 Be careful not to spill the contents of the microplate when you take the microplate holder out of the slot and when you remove the microplate from the holder Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 28 Chapter 6 Operation Fig 6 6 Removing the microplate holder pulling the holder out 3 Place the microplate holder on a heat resistant surface Open the cover and remove the microplate Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 29 Chapter 6 Operation temperatures reach 53 C 10 C in the unlikely event that temperature control to a holder is lost or enters a runaway condition Microplate holders at this temperature will be hot when touched To help prevent loss of temperature control ensure that the power contacts on the back of the microplate holder are clean and free from dirt or corrosion and
22. time Program the interval shaking time by dialing the number keys first minutes then seconds and finally hours It is recommended that the same interval time is used in all slots in one module If you need different interval times use slots in different modules o Note Check where the cursor underline is located before you enter a value 1 The value will be entered at the location of the cursor For example for an interval shaking time of zero SHAKE TIME 00 00 20 Press 00 00 J Enter INT TIME 00 00 00 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 20 Chapter 6 Operation the most recently started processing protocol Incorrect shaking speed may result if you start a protocol within the same module that has a shaking speed different from the other slots already in use To minimize the likelihood of this occurring it is recommended that you program all processing protocols within a module with the same shaking speed Note The slots within a module shake at the speed that is programmed into while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism The shaking speeds in revolutions per minute corresponding to the values you can enter are shown in the table below Table 6 1 Speed settings of the shaker Value 0 1 2 3 4 5 Shake speed rpm 0 400 650 900
23. with most laboratory detergents Dilute Clean the keyboard with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 4 Periodically inspect the connectors on the thermal microplate holders for dirt or corrosion If dirty or corroded wipe the connectors with a soft cloth slightly dampened with water 7 1 2 Immediate Wipe up all spills of liquids immediately from all surfaces of the instrument to prevent leakage into the electronics and mechanics of the instrument Clean and decontaminate the surfaces if necessary Although the iEMS Incubator Shaker is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 34 Chapter 7 Maintenance 7 2 How to replace fuses The fuses are located on the left side of the instrument Fig 4 1 item 8 1 Switch the instrument OFF Unplug the instrument and disconnect the power cable from the power input socket 2 Use a small flat screwdriver to pry the voltage selector fuse block assembly away from the power entry port housing and remove Fig 7 1 g m ysis
24. 18 Microplate in the thermal microplate hOldeTooooooococccacauacauaoooaonononononononononononononos 23 Inserting the thermal microplate holder into a SlOtciccccccccccctctetetstttttetssssststteeeees 24 Pushing down the microplate holder to latch it properly oooiioiiininnnccinicccccicccns 24 Removing the microplate holder lifting the handle ooocoocococccocacanananonannononononos 29 Removing the microplate holder pulling the holder OUt c ceccccceeeteeeeeees 29 Opening the voltage selector fuse block assembly oooooniiniininonocicicinannccccccccanano 35 FUSE DIOCK GSSCIMIDIV EE E ET 35 Installauon CheGReist ina nias eA ey ih eS Bi ee ee 14 Sp d settings OF THE SHAKEF saris ii a A dido 21 LED COIR A810 EEE AT A ne ei PN eh ae ae 22 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 6 Chapter 1 Safety Symbols and Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated Safety symbols and markings used on the iEMS Incubator Shaker Power ON C Power OFF SN Serial number REF Catalog number Date of manufacture Consult Instructions for Use WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A B Warning markings used in the documentation Warning Risk of
25. 2 item 4 rotate it 180 and replace Make sure that the correct voltage value is visible through the slit at the end of the voltage selector fuse block assembly Fig 5 2 item 3 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 16 Chapter 5 Installation ae T E y R ps N Fuse Fuse holder Slit showing the correct voltage Voltage selector module RUP Fig 5 2 Selecting the correct voltage 4 Slide the fuse block assembly back into the power entry port housing Snap the assembly securely into place 5 Reinsert the power cable 5 3 2 Mains supply cable connection Never use a mains supply cable other than the Thermo Warning Never operate your instrument from a power outlet that has no ground Scientific mains supply cable designed for your region 1 Ensure that the mains switch Fig 4 1 item 7 at the back of the left side panel is in the OFF position 2 Connect the mains supply cable to the mains input socket Fig 4 1 item 6 at the back of the left side panel 3 Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded 5 3 3 Setup Power on the instrument The firmware version is shown on the display Set the clock time 1 While in the READY state press C to select the setup menu 2 Select 1 for clock Press J Enter 3 Program the new time hh mm ss and press J 24 hour clock The baud rat
26. 4 2 3 shake OC start A _ aaa Fig 43 IEMS Incubator Shaker keyboard Listed below are the functions of the keyboard keys stop Stops the ongoing operation temp Sets the incubation temperature ON or OFF Used for starting preincubation preheating of the thermal microplate holders to the temperature set in processing protocol parameters shake Starts shaking with the speed and time set in processing protocol parameters start Starts operation defined by processing protocol parameters 0 9 Are used for entering slot numbers and parameter values C Clears an incorrectly keyed numeric value a Enters a keyed value or continues the program TEMPERATURE 37 0 C INC TIME 00 30 00 Fig 44 EMS Incubator Shaker display The alphanumeric liquid crystal display LCD has two 2 lines with up to 20 characters per line Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 12 Chapter 4 Functional Description 4 4 Thermal microplate holder PONS 5 Detachable cover with heating element Thermal and sensor contacts Bottom with heating element Adjustment screws Handle A1 Position of the A1 well on the microplate Fig 4 5 Thermal microplate holder The thermal microplate holder is designed for holding the microplate during the incubation process ensuring accurate temperature control o Note Adjust the screws Fig 4 5 item 4 with a screwdriver so t
27. 61000 4 6 2007 3 V 1 s 150 kHz 80 MHz EN 61000 4 8 1993 A1 2001 30 A m Criteria A EN 61000 4 11 2004 0 0 5 cycles Criteria B 0 1 cycle Criteria B 40 10 cycles Criteria B 70 25 cycles Criteria B 0 250 cycles Criteria C Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 47 Chapter 10 Ordering information 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Code 5112200 Instrument iEMS Incubator Shaker with nine 9 thermal microplate holders 220 240 V 5112207 iEMS Incubator Shaker with nine 9 thermal microplate holders 100 120 V 5921200 iEMS thermal microplate holder 10 1 List of accessories and recommended spare parts Code Item 1506200A EMS Incubator Shaker User Manual 1610420 Dust cover for iEMS Incubator Shaker 3 modules 5180190 RS Device link cable 1210070 1210550 Fuse 3 15A Power cord Euro 230 V 1210520 Power cord USA 100 V and 115 V 5921200 iEMS thermal microplate holder Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 48 Chapter 11 Warranty Certificate 11 Warranty Certificate Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against defective parts and materials including defects caused by poor wo
28. 6200A 57 Notes Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 58 Notes Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 59 Notes Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 60 Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2 P O Box 100 Fl 01621 Vantaa Finland www thermo com 15062004
29. A 9 18 programming Protocols mit aia 19 SHAKING ita ld 32 Ordering infortunio 48 P Packing disposal otimaterialS init aa tada dida 38 OA 38 SEL A A ed A E 15 Parameters Incubation t mPerature A A ads ees ai 19 INCUDA ION IMEI A ed ere eed idee 20 Intervalo A A tl 20 same Toral Slots ii AA A ad E AAA A in 19 A A dana cas dees toa Sane a gone aang po sass Ree a 19 SHAKING TIME bit A ee ee ee a etd ee de 20 VIG WWUING oc aczises dice stec Sens boised co s bani peas ceded A TEE 33 Placing the microplate into thermal microplate NOICED c ccceceeeeeeeeeeee cece ee ee tena aaaaeaeeeeeeeeeeaaeas 23 Pl A eg ee ee ee 9 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 51 Chapter 12 Index Power cables a eed hel fe cared ete Tes Seay Seng a nie O succeed ik as reads 17 A a a a E aa E a a EE decade 25 30 Program mMINg arier a ree eek At ea haa ie aa de Meee tay cede aaa ave eae a aa aa dd aa AAt 19 A ieee hese ence ae ace de ae dae ae ethene tape 19 Q Quick Reference QUIE cintia a A EEEE A EAE EREET Sea 54 R Res dahanede ash adiduvawtebvvat Sausitulivan E A E dadaauets 30 42 Ss SOVIET 34 43 packing tor dde 38 rEQUEest protocol srinasa ari ana ias 43 SELINA AMES A ad 19 SU A aa 17 Shakira ada 32 SPC OC PATEE EEE A ais h silos avd tin Valatd ais edGEMs aia bs E E oes ets saves evans seicad aad 25 39 41 ae a E chee dad eate cp thed ale teec dag cdb cai te ts 20 SITS OF operon A NN 15 DIOL
30. IA or ELISA Orbital shaking The shaking of a microplate in a circular movement with a fixed orbit and adjustable speed rpm Revolutions per minute 13 1 Keywords for web pages drug discovery EIA ELISA enzyme immunoassay immunoassay incubating incubator incubator shaker liquid handling microplate microplate incubator Microtiter plate microwell plate orbital shaker orbital shaking orbital shaking incubator plate separation technique s shaker shaking solid phase assay s Thermo Fisher Scientific Thermo Scientific Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 53 Appendix A Thermo Scientific EMS Incubator Shaker Quick Reference Guide Appendix A Thermo Scientific iEMS Incubator Shaker Quick Reference Guide 1 Place the microplate into the thermal microplate holder so that well A1 is located in the upper left corner when viewed with the numbers on the holder s handle the right side up Refer to Fig 6 2 2 Insert the thermal microplate holder into an incubation slot Refer to Fig 6 3 and Fig 6 4 Switch the instrument ON Refer to Fig 4 1 item 7 4 Program the incubation and shaking parameters for the appropriate slot Press the slot number 1 9 and Enter For all slots press 0 J Enter the incubation and shaking parameters in the following order refer to Section 6 2 and confirm each selection by pressing 4 Temperat
31. OOC A qu E E E deeeeddaveustbe one 50 Glossary and Abbreviations oooconnccccnnnoccccononcncconnnnnononnnnonnnnnnnn cnn n name nn nn rnnnnnrnrnnnnnnnnnnnns 53 Keywords for Web pageS cccconocccccononnncnannnncnannnnnncnnnnnnnrnannnnnrrnnn nn nrrnnn rra rrnnn nr nr nE Ennn nrrnnanss 53 Thermo Scientific EMS Incubator Shaker Quick Reference Guide 54 Certificate of Decontamination coocnnnnnnnnnnincnononananinoncnanananana nan nn nono naaa nan r nr anna nana 55 Thermo Scientific EMS Incubator Shaker Feedback Form c0c0csscceseeeseeees 56 aa le EL E ON 57 List of Figures Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 4 1 4 2 4 3 4 4 4 5 5 7 5 2 6 7 6 2 6 3 6 4 6 5 6 6 7 1 7 2 List of Tables Table 5 1 Table 6 1 Table 6 2 List of Figures and List of Tables IEMS I eubator Shaker front VOW seriene en ae a Nna 10 EMS Incubator ShaKker rear ViQW reter darei e aa nn nc cnn kaata i eein 11 EMS Incubator Shaker Keyboard isir risiedi iakie iriiri arira irine E E i i 12 IEMS Incubator Shaker display oooooocococonanononononononononononnnnonononononnnnnnnnnononononononononos 12 Thermal microplate holder 100 Ad Midweek 13 Opening the voltage selector fuse block assembly ooooociciicicnnonicicicannacocicncananos 16 Selecting ING CONECE VONAGC sa mepe gaea sh onde dh ts a a ae a 17 PHACIDICLOF opea O ito a Se sft bees tiie aaa e ida
32. Ordering Information and 11 Warranty Certificate In an effort to produce useful and appropriate documentation we appreciate your comments on this document to your local Thermo Fisher Scientific representative Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 8 Chapter 3 Introduction to the iEMS Incubator Shaker 3 Introduction to the iEMS Incubator Shaker The iEMS Incubator Shaker consists of three modules each module having a capacity of three 3 microplates at a time 3 1 Intended use The iEMS Incubator Shaker is a high quality standalone microplate incubator and orbital shaker intended for laboratory research use by professional personnel The instrument is used to incubate and shake microplates that meet the SBS standards with a temperature of up to 40 C and a shaking speed of up to 1400 rpm For verification of the entire system it is recommended that Good Laboratory Practices GLP be followed to guarantee reliable analyses Use for self testing is excluded 3 2 Principle of operation The iEMS Incubator Shaker consists of a keyboard and three 3 incubation modules Each module has three incubation slots for microplates All slots can be used for processing microplates individually within certain limitations A control LED above each slot indicates the status of that slot The keyboard is used to enter incubating and shaking parameters and to start thus programmed protocol
33. STATUS o E E sd e 22 Slot temperat E Abi 41 Slot Warmup iMac A A A AAA 41 SPECIFICATIONS A A A a 44 Jalil ca 44 INCUDALOF ii a E la td il 45 MV iii A EEE AA A DAA EA AAN a E Dd tias 46 Shaka O ia 45 Speed SettinO STi A A REEN a rd e ERS 21 Sl ie A A ade eb 34 40 Splashing ti A A DAA itis AE tite ai ti i 21 32 40 T Temp r t r setting ia ek Le cet 25 41 Thermal microplate holder rniii riasami ieaiaia apaa e adaini ariaa a anaia iea e 13 elenini iia deena need aie eek wie Ras 34 PIA CING Oi latas 23 Trans POrt damage reheat a a oe een ee act ale ee add 15 Transport package ta ed At Ad ait ats 14 15 U Unpaid Gee a el ei oe 14 V Viewing Parameters cui a aan tek aAa E DAE aAa data 33 40 Voltage Selec O ites hs a a a 16 W Wa MV A A A A A A A A 14 49 Med Diana 15 44 Working adecco a A aa e Fedea E data eles 2 15 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 52 Chapter 13 Glossary and Abbreviations 13 Glossary and Abbreviations EIA Enzyme immunoassay EN European Norm EU European Union IEC International Electrotechnical Commission Incubator An apparatus for maintaining optimal conditions temperature humidity etc for growth and development Microplate A rigid or framed polystyrene plate with microwells in different well formats for example 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as E
34. ation cononnnnncninincnoncnananinonanacananana cnn nn no nana an rar crac ananana nana 55 Thermo Scientific EMS Incubator Shaker Feedback Form 0000eeee 56 A A OO 57 Table of Contents Table of Contents 1 Safety Symbols and Marking cccccccccsssseceesseeeeeeeseeeeeenseeeeeeesseaeeeesseaeeeenseaeeeensneaes 7 2 About the User Manual mois ie 8 3 Introduction to the EMSTM Incubator Shaker oooococonocccnnococccnoconnnononnonncnnnnannnnnnannnnnos 9 3 1 intended Use ve Ai tana tas 9 3 2 Principle OF Operation idas 9 3 3 Advantages of using EMS Incubator Shaker oocccconncccccnanoncnnnanonnnnnanannncnnanonnnnnnnannnnnnns 9 4 Functional Descriptions c i 5 ccpeccesissasencdetcercesenssnenscceternssussnenetsdiecusanasnusdeecteress echenns 10 4 1 EMS Incubator Shaker front ViOW cccccccccceeeeeseeeeeeeseeneeeesseaeeesseaeeesseaeeeesenaeeesseaas 10 4 2 EMS Incubator Shaker rear ViW cccccceesceseeeeeeeeeeeeseenaeeesecaeeeesseaeeesseaeeeesenaneeeseaas 11 4 3 Keyboard and display esaiar ted esnips en hei ide a 12 4 4 Thermal microplate holder cccccceceeeeeeeeeee cece ener ee eeeaaeaeeeeeee eee eaaaaaaeaeeeeeseeeenneaaeeenees 13 5 talla dai ainda 14 5 1 Installation check lista nia aaa 14 5 2 Whatto do Upon delivery vicario 14 5 2 1 Howto unpack secta dildo dlls sac octee a N 14 5 2 2 Checking delivery for completeness occcnnoccccnonocccnnononccnnano conc n
35. by a dated and signed Certificate of Decontamination which must be attached to the outside of the package containing the instrument See Section 7 4 Decontamination procedure Failure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE 1 Please include decontaminating solution used Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 55 Appendix C Thermo Scientific
36. containing decontaminating solution and clean the surfaces of the instrument 5 Place the disposable towels into a biohazard container 7 4 2 How to decontaminate the thermal microplate holder In the event of spills of infectious agents on the surfaces of the thermal microplate holder perform the following decontamination procedure 1 Use disposable gloves 2 Absorb the spill with disposable towels 3 Dampen a disposable towel with 2 glutaraldehyde containing decontaminating solution and clean the surfaces of the thermal microplate holder 4 Place the disposable towels into a biohazard container If the abovementioned cleaning procedure is insufficient because of the extensiveness of the spill of infectious agent perform the following decontamination procedure 1 Place the thermal microplate holder into a container containing decontaminating solution with 2 glutaraldehyde for 30 minutes or according to the directions provided by the manufacturer of the decontaminating solution 2 Rinse the thermal microplate holder with water 1 Ref National Committee for Clinical Laboratory Standards Protection of Laboratory Workers from Infectious Disease Transmitted by Blood Body Fluids and Tissue Tentative Guideline NCCLS Document M29 T2 Vol 11 No 14 Villanova Pa NCCLS 1991 Ref National Committee for Clinical Laboratory Standards Protection of Laboratory Workers from Instrument Biohazards Proposed Guideline
37. d of according to local waste regulations The Li batteries have to be changed by an authorized service technician only Instructions for changing the Li batteries are described in the service manual Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS Regarding the original packaging and packing materials use the recycling operators known to you For further information contact your local Thermo Fisher Scientific representative Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 38 Chapter 8 Troubleshooting Guide 8 Troubleshooting Guide A Warning Do not use the instrument if it appears that it does not function properly 8 1 Limitations Control LEDs The orange control LED light emitting diode flashes when a slot is heating up to the programmed target temperature Refer to
38. e is used for service purposes only If you want to interrupt the setup press stop to return to the READY state Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 17 Chapter 6 Operation 6 1 Principle of operation When you power on the instrument it will be in the READY state From the READY state you can choose to modify the setup parameters Section 5 3 3 Setup or the protocol parameters Section 6 2 Programming protocols If you have already programmed protocols while in the READY state you can start to preheat the required slots Section 6 5 Preheating and heating before starting the operation Section 6 4 Normal operation It is also possible to start shaking the slots without heating as a separate function Section 6 6 Shaking For an overview of the operations see Fig 6 1 below Slot requires Start Change protocol f preheating protocol READY parameters Shaking only 1 9o0r0 Slot no 1 90r0 Slot no for all slots for all slots Slot no 1 90r0 1 9o0r0 for all slots J Enter for all slots Set protocol HEAT ON parameters SHAKING READY Protocol starts see 6 2 ON READY READY READY Fig 6 1 Principle of operation Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 18 Chapter 6 Operation 6 2 Programming protocols Use the keyboard to enter processing parameters and to start thus programmed protocols You can program a
39. electric shock AN Warning Biohazard risk A Warning Hot surface risk of burns A Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application Other markings used in the documentation o Note Marks a tip important information that is useful in the optimum 1 operation of the system or an item of interest Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 7 Chapter 2 About the User Manual 2 About the User Manual The user manual has been written for the actual end user for example laboratory technician and provides information on the Thermo Scientific iEMS Incubator Shaker including installation and operating instructions Read the manual in its entirety prior to operating the instrument The user manual has been designed to give you the information you need to e Review safety precautions e Install the EMS Incubator Shaker e Carry out incubating and shaking procedures e Perform basic maintenance procedures e Troubleshoot the instrument performance e Maintain the instrument The user manual also describes features and specifications of the EMS Incubator Shaker hardware and onboard software Chapter 6 Operation explains the operating procedures The user should be familiar with the contents of Chapter 7 on maintenance For warranty and ordering information refer to Chapters 10
40. fications General specifications Overall dimensions 400 mm W x 450 mm D x 400 mm H 15 7 in W x 17 7 in D x 15 7 in H Weight total 30 kg 66 lbs Mains power supply 100 120 Vac 60 Hz nominal 200 240 Vac 50 Hz nominal Power consumption 380 VA max Heat dissipation 1297 BTU max Fuses 2 x T3 15 A 250 V 5 x 20 mm IEC 127 3 IIl Operating conditions indoor use 10 C 40 C RH 90 Transportation conditions 40 C 70 C packed in transport packaging Storage conditions 25 C 50 C packed in transport packaging Microplate dimensions Maximum 86 mm W x 128 mm L x 15 mm H Device interfaces 1 Serial RS 232C interface for computer 2 Serial RS 485 interface for other device link Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 44 9 2 Incubator specifications Chapter 9 Technical specifications Performance of the incubator with flat bottomed microplates with nominal voltages Programmable temperature range 14 C 40 C Controlled incubation range Ambient plus 3 C to 40 0 C Resolution 0 1 C Programmable incubation time Up to 48 hours in steps of 1 second Slot warmup time lt 20 minutes to reach a 37 C 1 0 C target temperature in the empty plate carrier when starting from ambient temperature approx 24 C
41. gases are present 5 2 5 Things to avoid Do not smoke eat or drink while using the iEMS Incubator Shaker Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Ensure that the working area is well ventilated Never spill fluids in or on the equipment Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 15 Chapter 5 Installation 5 2 6 Technical prerequisites Place the instrument on a normal laboratory bench The net weight of the instrument is 30 kg 66 lbs The instrument operates at voltages of 100 120 Vac 220 240 Vac and the frequency range 50 60 Hz 5 3 Setups before you put the instrument into operation 5 3 1 Voltage selector The voltage selector fuse block assembly Fig 5 1 is located on the left hand side Fig 4 1 item 8 of the instrument Check that the selected voltage corresponds to the local voltage You can see the current voltage setting through the slit in the fuse holder Fig 5 2 item 3 To change the selected voltage 1 Remove the power cable from the power inlet 2 Use a small flat screwdriver to pry the voltage selector fuse block assembly away from the power entry port housing and remove Fig 5 1 amy 2 Fig 5 1 Opening the voltage selector fuse block assembly 3 Remove the voltage selector module Fig 5
42. hat the microplate is held firmly in place during shaking Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 13 Chapter 5 Installation 5 Installation 5 1 Installation check list The chapter on installation will contain an outline of the points mentioned in the check list below Table 5 1 Installation check list Tick Item Unpack the iEMS Incubator Shaker instrument carefully Refer to 5 2 1 Keep the original packaging and packing material for future transportation Check the delivery for completeness Refer to 5 2 2 Check for damage during transport Refer to 5 2 3 Place the instrument on a normal laboratory bench taking into account both the climatic and technical prerequisites Refer to 5 2 4 and 5 2 6 Leave sufficient clearance on both sides and at the rear of the unit Install the instrument Refer to 5 3 OO O OD O Connect the mains supply cable to the mains input socket Refer to 5 3 2 5 2 What to do upon delivery 5 2 1 How to unpack Move the unpacked instrument to its site of operation Unpack the EMS Incubator Shaker instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package e Warranty Certificate card e packing instructions packing list e transportation damage and discrepancy report e Manufacturing Quali
43. iEMS Incubator Shaker Feedback Form Please send to Thermo Fisher Scientific Oy Fax 358 9 32910415 Appendix C Thermo Scientific EMS Incubator Shaker Feedback Form Cat no Serial no PURCHASED BY PURCHASED FROM Company Organization Distributor Department Address Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr O Mr O Mrs O Ms O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions o O O a Instrument User manual O O O O Operational reliability O Oo Oo O Design a O O O Ease of use O O o o Operational costs O o o o Customer support O O O o Further instrument system developments desired Further applications desired Where did you first learn about the product Would you like to receive information about other Thermo Scientific products Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 56 Appendix D Addresses Appendix D Addresses For the latest information on products and services visit our websites at http www thermo com Manufactured by Distributed by Thermo Fisher Scientific Oy P O Box 100 FI 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 150
44. iate slot and then start to start the protocol For example to start slot 1 PLACE 1 Press 1 start The protocol will start and the LED will light up with a red color 09 26 30 The READY state appears while the instrument is shaking This may damage the shaking a Caution Do not place the microplate holder in or remove it from the instrument mechanism 6 4 3 2 Checking the operation time During operation you can check the remaining incubation time by pressing the slot number For example Press 1 If the remaining time is 23 minutes and PLACE 1 15 seconds the display will show TIME LEFT 00 23 15 Press C to return to the READY state 6 4 3 3 Starting a program during operation in another slot Within a module if a slot has a protocol in progress with a shaking speed of 0 another protocol with PLACE 2 shaking will not start and the display will show PROHIBITED OPERATION o Note The slots within a module shake at the speed that is programmed into the most recently started processing protocol For example if there is a protocol in progress with speed 5 and the adjoining slot is started with speed 3 all slots within the module will shake at speed 3 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 26 Chapter 6 Operation Within a module a second protocol with a programmed temperature different from the protocol already in PLACE 3 progress will not start and
45. ing protocol When inserting a microplate holder into a slot push the holder completely in and down to latch it into its normal operating position If the microplate holder is not latched into position it may lose contact with the temperature controlling connectors at the rear of the slot Refer to Section 6 4 1 Instrument reset due to power loss If the power to the instrument is lost the instrument will reset to the READY mode when the power is restored All shaking and incubation functions including timing will be turned off No LEDs will be lit and the current time of day will be shown on the display During shaking Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism 8 2 6 Maintenance AN Turn power off and unplug instrument during maintenance procedures To prevent electric shock turn the power off to the instrument and unplug the power cable during maintenance procedures Dirty thermal microplate holder contacts Ensure the power contacts on the back of the thermal microplate holder are clean and free from dirt or corrosion Dirty contacts may affect temperature control of the microplate holder in the slot Corrosive materials Do not use corrosive materials during maintenance procedures Refer to Chapter 7 Maintenance in this manual for information on maintenance procedures Painted surfaces can be cleaned with most laboratory de
46. ion and shaking parameters of a Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 33 Chapter 6 Operation Warning To prevent electrical shock turn the power off to the instrument and unplug the power cable during maintenance procedures 7 1 Regular and preventive maintenance 7 1 1 General Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied For reliable daily operation keep the instrument free of dust and spills from liquids It is also advisable to cover the instrument with the dust cover supplied when not in use In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended that you clean the case of the instrument periodically to maintain its good appearance A soft cloth dampened in a warm mild detergent solution will be sufficient Clean the outside of the instrument and the thermal microplate holder with clean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Caution Painted surfaces can be cleaned
47. is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Thermo Fisher Scientific representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department The Warranty Claim Technical Sheet contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill out and forward the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department Check Section 7 5 How to pack for service You will find instructions on how to proceed before shipping the instrument for service Check that any necessary decontamination procedure has been carried out before packing See Section 7 4 Decontamination procedure Ensure the Certificate of Decontamination see Appendix B as well as the return authorization number RGA are sent with the instrument The Thermo Fisher Scientific technical service department will keep you up to date with the progress of service and provide you with any further details you might need for example on maintenance serviceability troubleshooting and replacement Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 43 Chapter 9 Technical specifications 9 Technical Specifications 9 1 General speci
48. ncnnnnnnnnnnnnnanecccnnnnnnns 37 How To pack TOF ServicE iria da plc dit 38 Disposal of the instrUMeNt oonocccnnnnoccccnononnnnnonannnnnnn non nnnnn nan n nn r nn r nn ranma rra rra 38 Troubleshooting Guide coooooccccnncocccnnnnocccononocnnnnnnnnnonnnnnnnonrnnnnn rr nrnnn nn rr rnnn nn rr nennen rr rana anno 39 LIMITATIONS gan rona td de dae de T 2 39 Warnings And Caution tara aE eE E AE A EEL EE 39 Installation td id A A 39 General nc ii a Ad dise 40 TEMPE ci an AEE En AEE 41 ShakiNgis iia add tada 41 OPEN A a a 42 MalNten aC ito a IA Ponape in e a 42 Service request protocol ico cidad idad diia daci n dd dd dad 43 Technical SpecificatiONS oooocoonnoconnnooncnnnconcnnnnnonnnononnnnncnnnnnnnnrrnnnnnnrrnna nn nr nennen nennen nanena 44 General specifications secme iaria reunaa rra a rr eein erkan erdirir 44 Incubator SPECIFICATIONS merisead aa rra rra 45 Shaker SpeCifications serres erp anane eaea enu E E KAEKA EERE PARRAS NE KEEA N KEETE rr naar ra NAE PEANY 45 Safety Specification e da A OEL EAE 46 In conformity with the requirementS nnocccnnnicccnnonocnnnnonannnnnnnonnn nn nr nnnn nn anna nn rr rrnnn rra 47 Ordering Information cccccccesseceesseeeeeesseeeeeenseaeeeeeseaaeeeesseaeeesnseaeeeseseaeeeesseaeeeenaaes 48 List of accessories and recommended spare Palts c cccccecsseeeceesteeesseneeeesseneeeesenas 48 Warranty Certificate cional ia ia ida aii 49 Warranty llMitations silla iria ahi add 49 O APP NU
49. nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Thermo Fisher Scientific representative 5 2 4 Climatic requirements When you set up your iEMS Incubator Shaker avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables reagent bottles etc e Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make sure the ambient temperature range is between 10 C 50 F and 40 C 104 F e Make sure relative humidity is between 10 and 90 non condensing The iEMS Incubator Shaker does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Warning Do not operate the instrument in an environment where potentially damaging liquids or
50. plate holder For effective mixing a powerful variable speed orbital shaker is incorporated Featuring an orbit of 1 mm and speeds from 400 to 1400 rpm in 250 rpm increments the shaker motion ensures efficient mixing of even very viscous liquids All slot positions within a module are shaken simultaneously Each slot of the iEMS Incubator Shaker has a control LED light emitting diode that displays the status of the slot The LED will change to a specific color based on the status of the slot When the incubation and shaking operations end the instrument also gives an audio signal 4 1 iEMS Incubator Shaker front view Display Keyboard Control LED light emitting diode Incubation slot Incubation module with three 3 slots Mains input socket Mains switch Voltage selector and fuses Fig 4 1 EMS Incubator Shaker front view DN OOP ON gt Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 10 Chapter 4 Functional Description 4 2 EMS Incubator Shaker rear view 1 1 1 Cooling air inlet DIP switches not in use Device link RS 485 not in use Computer RS 232C device link RS 485 not in use Cooling air outlet Type label DOP ON Fig 4 2 EMS Incubator Shaker rear view Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 11 Chapter 4 Functional Description 4 3 Keyboard and display 7 8 9 stop 4 5 6 temp
51. quirements iEMS Incubator Shaker bears the following markings Type 1410 100 120 Vac 200 240 Vac 50 60 Hz 380 VA max CE mark CSA monogram with US designation iEMS Incubator Shaker conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B 2002 96 EC Waste of Electrical and Electronic Equipment 2006 66 EC Directive on batteries and accumulators and waste batteries and accumulators Safety performance EN 61010 1 2001 Ed 2 and including CA US National Differences EN 61010 2 010 2003 Ed 2 EMC performance EN 61000 6 3 2007 Generic emission standard Residential commercial and light industry EN 61000 6 1 2007 Generic immunity standard Residential commercial and light industry EN 61326 1 2006 Product family standard Test standards Performance limits EN 55011 1998 A1 1999 A2 2002 Class B 150 kHz 1 GHz EN 61000 3 2 2006 EN 61000 3 3 1995 A1 2001 A2 2005 ANSI C63 4 2003 Class B 450 kHz 1 GHz 30 MHz 1000 MHz EN 61000 4 2 1995 A1 1998 A2 2001 4 kV CD 8 kV AD 4 kV ICD Criteria B EN 61000 4 3 2002 A1 2002 EN 61000 4 4 2004 3 V m 80 MHz 2 7 GHz Criteria A 0 5kV 1kV 2kV Criteria B EN 61000 4 5 2001 0 5kV 1kV 2kV Criteria B EN
52. rkmanship for a period of twelve 12 months from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions The warranty is invalid if products have been misused or abused For this warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the character listed above e Refurbished products that are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connection with the use of the product or other indirect damages Full warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer 11 1 Warranty limitations Consumables are
53. s These protocols can be the same for all slots or entered individually for each slot The parameters are stored in the instrument memory until new parameters are entered It is possible to change the parameters of one slot without affecting the parameters of the other slots In one module all slots have the same incubation temperature and they shake at the same speed The slots should be preheated to the programmed target temperature before protocols are started 3 3 Advantages of using EMS Incubator Shaker The iEMS Incubator Shaker provides several advantages relating mainly to the principle of operation in that it has e Exceptional temperature uniformity across the plate e Efficient orbital shaking e Capacity of up to nine 9 microplates Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 9 Chapter 4 Functional Description 4 Functional Description The iEMS Incubator Shaker uses an individual thermal microplate holder designed for ease of use and uniform heating of each microplate Temperature variation is less than 0 3 C across the microplate To eliminate temperature gradients and edge effects microplates are evenly heated from all sides This ensures assay reproducibility Both the lid and the bottom of the thermal microplate holders are heated electrically via connectors in the plate carrier An upper heater within the top of the module prevents condensation from forming within the micro
54. t any processing protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange Refer to Section 6 3 Slot temperature Before you start the processing protocol ensure the temperature of the slot is within the acceptable range appropriate for the application to be carried out You can view the actual slot temperature on the instrument display Refer to Section 6 5 8 2 4 Shaking Module shake speed set by the most recently started processing protocol The slots within a module shake at the speed that is programmed into the most recently started processing protocol Incorrect shaking speed may result if you start a protocol within the same module that has a shaking speed different from the other slots already in use To minimize the likelihood of this occurring it is recommended you program all processing protocols within a module with the same shaking speed Refer to Sections 6 2 and 6 4 3 3 During shaking Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 41 Chapter 8 Troubleshooting Guide 8 25 Operation Ensure the thermal microplate holder is latched into its normal operating position Ensure you have inserted the thermal microplate holder correctly into the slot before you start the process
55. t handle the thermal microplate holder from its cover since it will come off Use the handle Fig 6 2 Caution Do not place the microplate holder into the instrument while the instrument is shaking This may damage the shaking mechanism Fig 6 2 Microplate in the thermal microplate holder Be careful not to spill the contents of the microplate when you load it into the thermal microplate holder and when you insert the holder into the slot Carefully insert the plate into the microplate holder and close the cover Caution The covers of the thermal microplate holders are not spring loaded but you should take care not to trap your fingers when closing the cover of the holder Avoid bumping the microplate holder while inserting it into the slot Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 23 Chapter 6 Operation When you are inserting a thermal microplate holder into a slot push the holder completely in Fig 6 3 and then down Fig 6 4 to latch it into its normal operating position Caution Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Fig 6 3 Inserting the thermal microplate holder into a slot Fig 6 4 Pushing down the microplate holder to latch it properly o Note If the thermal microplate holder is not latched into position it may lose I contact with the temperature controlling
56. tergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Decontamination If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 4 The samples can be potentially infectious Dispose of all used microplates disposable gloves syringes disposable tips etc as biohazardous waste Only use a cloth dampened with a decontaminant to decontaminate the instrument The use of spraying may damage the instrument If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect the enzyme being used in EIA tests in a negative way resulting in bad test results Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 42 Chapter 8 Troubleshooting Guide Always use disposable gloves and protective clothing and operate in a well ventilated area Decontaminate the instrument prior to disposal See Section 7 4 and Appendix B on decontamination 8 3 Service request protocol If the EMS Incubator Shaker requires service contact your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Do not under any circumstances send the instrument for service without any prior contact It
57. the display PROHIBITED OPERATION will show When the protocol is completed the LED will light up with a green color and you will hear a beeping sound The heating stays on For turning off the beeping sound see Section 6 4 3 6 6 4 3 4 Checking the operation stop time Before taking the thermal microplate holder out of the slot you can check the actual time of day when the incubation stopped Press the slot number 1 9 For example Press 1 PLACE 1 INC STOP 09 20 31 Press C to return to the READY state If you take the thermal microplate holder out of the slot you can no longer check the time indicating the stop of the incubation 6 4 3 5 Stopping the operation If you press a slot number and stop before the processing protocol time elapses the protocol stops and incubation stays on The LED will light up with a green color and you will hear a beeping sound For turning off the beeping sound see Section 6 4 3 6 The READY state appears 09 27 30 6 4 3 6 Turning off the audio signal while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism If you want to turn off the audio signal but wish to continue the incubation proceed as follows 1 Make sure the control LED is green and that the module is not shaking 2 Remove the thermal microplate holder from the slot The audio signal and the control LED are t
58. tion 6 7 Verifying incubation and shaking parameters Splashing from overfilled wells Splashing may occur if you use shaking with wells that contain too much liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3 in most cases Refer to Section 6 2 Programming protocols Splashing aerosols and evaporation To minimize the possibility of splashing aerosols and evaporation during shaking and incubation it is recommended you use microplate sealers Spills Wipe up all spills of liquids immediately from all surfaces of the instrument to prevent leakage into the electronics and mechanics of the instrument Clean and decontaminate the surfaces if necessary Refer to Section 7 1 2 Incubation time Do not remove the thermal microplate holder until the incubation time has elapsed If you remove the microplate holder from the slot before the incubation time has elapsed the incubation timer does not stop and continues to count down causing tracking of actual incubation time to be lost Refer to Section 6 4 3 6 During shaking Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Thermo Scientific EMS Incubator
59. to be carried out You can view the actual slot temperature on the instrument display see Section 6 5 Preheating and heating Changing programmed temperature setting When you change the programmed temperature setting ensure that the slot temperature is at the new target setting before you start the processing protocol The instrument does not prevent the processing protocol from starting if the slot is not at the target temperature You can view the actual slot temperatures on the instrument display See Section 6 5 Preheating and heating Module shake speed All slots within a module will shake at the same speed The slots within a module shake at the speed that is programmed into the most recently started processing protocol Incorrect shaking speed may result if you start a protocol within the same module that has a shaking speed different from the other slots already in use To minimize the likelihood of this occurring it is recommended you program all processing protocols within a module with the same shaking speed Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 25 Chapter 6 Operation 6 4 3 Operation 6 4 3 1 Starting the operation contact with the temperature controlling connectors at the rear of the de Caution If the thermal microplate holder is not latched into position it may lose slot see Section 6 4 1 Placing the microplate Operation Display Press the number of the appropr
60. ty Report e EMS Incubator Shaker User Manual Caution Do not touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty To lift the instrument put your fingers under the bottom on both sides and lift it with your back straight Caution When unpacking the instrument it is recommended that two people lift the instrument together taking proper precautions to avoid injury Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 14 Chapter 5 Installation 5 2 2 Checking delivery for completeness Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative 5 2 3 Checking for damage during transport Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer
61. ure C Incubation time hh mm ss Shaking time hh mm ss Interval time hh mm ss Shaking speed as shown below Value Shake speed rpm 0 400 650 900 1150 1400 5 Press temp followed by the slot number to start the preheating of the thermal microplate holder Refer to Section 6 5 6 Wait until the control LED changes from a flashing orange to a continuous orange 7 Press the slot number 1 9 and then start to start the protocol The protocol will start and the LED will light up with a red color 8 When the protocol is completed the LED will light up with a green color and you will hear a beeping sound The heating stays on 9 To turn off the heating press temp followed by the slot number 1 9 and then stop 10 Remove the thermal microplate holder from the slot when the module is not shaking while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism 11 Turn the instrument OFF Thermo Scientific iEMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 54 Appendix B Certificate of Decontamination Appendix B Certificate of Decontamination The decontamination procedure is required prior to shipping the instrument to Thermo Fisher Scientific Oy for example for repair If for any reason the instrument is shipped back to Thermo Fisher Scientific Oy it must be accompanied
62. urned off and the heating of the slot is turned off 3 Re insert the microplate holder into the slot The orange LED is turned on and the heating of the slot turns back on Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 27 Chapter 6 Operation If one of the slots has completed the protocol but another slot in the same module is still shaking you can turn off the audio signal as follows 1 Press temp n stop where n is the slot number that has completed the shaking The audio signal and the control LED are turned off and the heating of the slot n is turned off The module continues to shake until the programmed time of the other slots in the module has elapsed It is recommended to use the same shaking time in all slots in one module to avoid these kinds of situations If you want to stop the heating and the shaking of the module but do not want to remove the microplate holder yet from the instrument proceed as follows 1 Press shake stop The shaking of the module will stop 2 Press temp stop The heating of the module will stop and the green LED and the audio signal will turn off 6 4 4 Removing the microplate Caution Do not remove the microplate holder from the instrument while the instrument is shaking This may damage the shaking mechanism 1 Wait until the slot s control LED is green and you hear the audio signal The processing protocol is complete but the heating of the slot is
63. ve been in direct contact with any part s of the instrument If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended the complete decontamination procedure is performed before relocating the instrument from one laboratory to another Refer to Section 7 5 How to pack for service Decontamination is not required for the proper functioning of the instrument Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect the enzyme being used in EIA tests in a negative way resulting in bad test results Warning Always use disposable gloves and protective clothing and operate in a well ventilated area Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 36 Chapter 7 Maintenance 7 4 1 How to decontaminate the instrument surface In the event of spills of infectious agents on the surfaces of the instrument perform the following decontamination procedure 1 Switch the power off and unplug the instrument 2 Use disposable gloves 3 Absorb the spill with disposable towels 4 Dampen a disposable towel with 2 glutaraldehyde
64. y slot the PLACES 1 9 display will show HEAT ON Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 30 Chapter 6 Operation Operation Display If the heating is OFF in every slot the PLACES 1 9 display will show HEAT OFF If some slots are ON and some are PLACES 1 9 OFF the display will show HEAT ON OFF At this point press start to turn the heating ON in all slots or press stop to turn the heating OFF in all slots Press C to return to the READY state 09 29 10 Thermo Scientific EMS Incubator Shaker User Manual Rev 3 4 Cat no 1506200A 31 Chapter 6 Operation 6 6 Shaking while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism much liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting Caution Splashing may occur if you use shaking with wells that contain too When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3 in most cases Operation Display You can activate shaking without heating as a separate function by pressing the shake key PLACE NUMBER Press shake Press the slot number 1 9 For example PLACE 1 Press 1 900 rpm 00 00 20 To start shaking

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