Hybex Microarray Incubation System User Manual
Contents
1. 9 Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 3 408 733 7337 techserv scigene com I SAFETY NOTICES A Warnings Failure to comply with the following warnings that are affixed to the product can lead to possible personal injury or death B Cautions Failure to comply with the following cautionary statement affixed to the product may lead to possible personal injury Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 4 408 733 7337 techserv scigene com II UNPACKING AND SET UP A Unpacking All components of the system are supplied in a single carton with three interior cartons Open the largest interior carton which contains the heating base Remove the packaging material around the unit and lift it out taking care not to damage the hinged cover Unpack the remaining two small interior cartons which contain the incubation chambers Carefully inspect the heating unit carefully for damage If there is evidence of damage do not discard the shipping materials since they may be needed to return the unit B Parts Provided The following items are provided with the system Please inspect to ensure that all components are included Hybex Microsample Incubator heating base User Manual Power Cord Microarray Incubation Chambers Two Complete Sets Each Set Provided Includes o Microarray Slide Racks 2 Shipped inside chamber o2. C Using the Temperature Controller The temperature controller has a single LED read out and four push buttons The LED displays the set temperature when the SET key is pressed and held Otherwise the ACTUAL block temperature is displayed The push buttons are used to set the block temperature and when required to calibrate the temperature controller 1 To set the block temperature hold in the SET button The previous set temperature will be displayed 2 While depressing the SET button press the up or down arrow buttons until the desired set temperature is shown 3 Release the buttons and the actual block temperature will again be displayed The unit will now adjust the heat of the block until the new set temperature is attained The controller is calibrated at the factory to provide an accurate block temperature when operated between 35 to 99 C Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 7 408 733 7337 techserv scigene com D Calibrating the Temperature Controller The temperature controller comes calibrated from the factory to provide accurate block temperatures from 35 to 99 C The temperature controller will require calibration only if When checking the block temperature with a calibrated digital thermometer the block temperature differs by more than one degree 1 C from the actual temperature shown on the controller display An NIST calibrated digital thermometer sold se
3. Controls and Accessories 5 B Handling the Incubation Chambers 6 C Using the Temperature Controller 6 D Calibrating the Temperature Controller 7 IV MAINTAINING YOUR HYBEX SYSTEM 8 A Powering Off 8 B Checking and Replacing Fuses 8 C Cleaning 8 V TROUBLESHOOTING 9 VI SPECIFICATIONS 9 VII ORDERING INFORMATION 9 A System Components 9 B Optional Tube Blocks and Accessories
4. T handled allen wrench for assembling chambers o Handle for duplexed slide racks o Four pronged screw in handle for chambers o Pack of replacement absorbent pads for chamber cover 25 o Hybex Microarray Incubation Chamber User Guide C Installation Place the heating unit on a level surface within a few feet of the power source The unit should be positioned to have clearance along the top so that the lid can be opened completely without interference Leave at least 3 inches of clearance from the back panel for air circulation Assemble and insert both incubation chambers into the heating unit See Section III for how to handle chambers Both incubation chambers must be inserted into heating unit before turning on power Plug the power cord provided into the back of the unit and then to a properly grounded outlet Use only the power cord provided Turn on power to the system using the switch on the front of the unit Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 5 408 733 7337 techserv scigene com III USING YOUR HYBEX SYSTEM A Components Controls and Accessories Name Function Power Switch Turns on main power to unit Temperature Controller Used to set and observe block temperature Heated Lid Heats the sealed air around the block Temperature Probe Senses the temperature of chambers blocks Digital Thermometer Jack For calibrating system temperature Microarray Incubation Ch
5. amber For array hybridization Heat Block optional Heats tubes and plates without cap condensation Digital Thermometer optional For calibrating system temperature Waterbath Insert optional For heating buffers or water Temperature Controller Power Switch OFF ON WATLOW S D31 Temperature Probe Heated Lid C F JENCO MAX MIN RESET 7001H Microarray Incubation Chamber Heat Block Waterbath Insert Digital Thermometer Digital Thermometer Jack Power Cord Receptacle BACK OF UNIT Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 6 408 733 7337 techserv scigene com B Handling the Incubation Chambers See the Hybex Microarray Incubation User Guide included with each chamber set for details on how to use the slide racks and assemble the chamber The following information is limited to how to insert and remove the chambers from the heating unit Chambers are inserted and removed with the aid of a single four pronged black plastic handle provided 1 Thread handle several turns into the chamber cover 2 Lower the assembled chamber into the unit aligning the cutout on the side of the chamber with the temperature probe in the base 3 Repeat with second chamber 4 Remove the handles from the chamber turn on power and set desired temperature See next section Do not operate the system without both chambers inserted into the heating unit
6. ion Chamber Cover and Base Each B Optional Tube Blocks and Accessories Cat Description UoM 1057 31 0 PCR Tube block holds 96x0 2 ml tubes or single plate Each 1057 33 0 0 6mL tube heat block holds 60x0 6mL tubes Each 1057 34 0 1 5mL tube heat block holds 32x1 5mL microcentrifuge tubes Each 1057 35 0 Waterbath Insert for Hybex heating unit Each 1051 52 0 Digital thermometer Includes cable and NIST certificate Each
7. m loss arising out of or in connection with the sale use or performance of the product Copyright Copyright 2004 2007 SciGene Corporation All rights reserved SciGene and Hybex are trademarks of SciGene Corporation Sunnyvale CA All other trademarks used in this manual are the property of their respective owners Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 2 408 733 7337 techserv scigene com Table of Contents I SAFETY NOTICES 3 A Warnings 3 B Cautions 3 II UNPACKING AND SET UP 4 A Unpacking 4 B Parts Provided 4 C Installation 4 III USING YOUR HYBEX SYSTEM 5 A Components
8. on process starting at Step 1 for the highest temperature for the range of temperatures you will be using Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 8 408 733 7337 techserv scigene com IV MAINTAINING YOUR HYBEX SYSTEM A Powering Off Turn the power switch to the OFF position and unplug the power cord before performing any service procedure B Checking and Replacing Fuses There are two fuses located in a removable fuse block below the power cord receptacle on the back of the unit Insert a small flat blade screwdriver into the tab recess just below the plug receptacle Push down to release the fuse block Slide out the fuse holder from the fuse block while holding the retaining tab out of the way Gently pry out the fuses A blown fuse appears dark Always replace fuse s with those of the same amperage and voltage as shown on the label below the fuse block C Cleaning Clean the metal surfaces and heated lid using a mild detergent based spray cleaner and wipe with a soft cloth Avoid abrasive cleaners that can scratch surfaces The bottom of the chamber is sealed and will retain spills and condensation Clean the chamber and temperature probe frequently to avoid build up of residue that can interfere with the heat control system Back of unit Use small screwdriver behind tab to open Fuse Block Fuse Block Push clip up to release Fuse Holder Digital Thermometer Jack Hybe
9. parately Cat 1051 52 0 is required to calibrate the unit Follow these steps to adjust the controller to achieve accurate temperatures 1 Set the temperature on the controller to 65 C and allow the temperature in the unit to stabilize 2 Using the cable provided with the digital thermometer SciGene Cat 1051 52 0 plug one end into the blue receptacle found on the back panel of the unit and the other into the digital thermometer 3 Turn on the thermometer using the ON OFF button on the keypad The temperature of the block will be displayed 4 On the temperature controller press the infinity key for three seconds until OPEN appears 5 Press the down arrow four times until Cal appears 6 Press and hold the set key The existing offset value between the controller and digital thermometer is displayed 7 Calculate the difference in the temperature shown on the controller and the digital thermometer e g the controller displays 52 5 C and the digital thermometer displays 51 0 C the difference is 1 5 C 8 Press and hold the SET key and use the up and down arrows to enter the offset value calculated in Step 7 For example if the controller displays a temperature that is 1 5 C higher than the digital thermometer adjust the offset value to minus 1 5 1 5 9 Press the Infinity Key to exit calibration The block temperature is now calibrated for the set temperature you selected Repeat the calibrati
10. www scigene com SciGene 306 Potrero Avenue Sunnyvale CA 94085 USA 408 733 7337 Fax 408 733 7336 techserv scigene com Hybex Microarray Incubation System USER MANUAL Cat 1057 36 1 1057 36 2 FOR RESEARCH USE ONLY Not for Use in Diagnostic Procedures Hybex Microarray Incubation System USER MANUAL Version 1 0 January 2006 1 408 733 7337 techserv scigene com Serial Number The following serial number identifies the specific instrument you have purchased and must be referenced when requesting service A copy is affixed to the instrument Technical Service 408 733 7337 techserv scigene com Warranty SciGene warrants that the heating unit described in this manual shall be free of defects in materials and workmanship for a period of 12 months from date of delivery This warranty does not cover removable blocks or accessories In the event of a defect during the warranty period SciGene s limit of liability will be to provide replacement parts at no charge or at its sole discretion replace the product The foregoing warranty is void in the event the unit was abused or modified or used in a manner inconsistent with its intended purpose SciGene makes no other warranty expressed or implied including warranties of merchantability and fitness for a particular purpose In no event shall SciGene be liable for any direct indirect special incidental or consequential damages or for any damages resulting fro
11. x Microarray Incubation System USER MANUAL Version 1 0 January 2006 9 408 733 7337 techserv scigene com V TROUBLESHOOTING Symptom Cause Solution Power switch light does not turn on Blown fuse s Replace fuse s on back of unit beneath power cord receptacle VI SPECIFICATIONS Electrical Cat 1057 36 1 115V AC 50 60 Hz 250W Cat 1057 36 2 220V AC 50 60 Hz 250W Dimensions Outside cover closed H x W x D 8 x 6 x 12 inches 12 x 14 x 30 cm Weight Net 5 2 lbs 2 4 kg Gross in shipping carton 4 1 to 6 0 lbs 1 9 to 2 7 kg Performance and Controls Temperature Range Ambient 5 C to 99 C Temperature Regulation 0 2 C Heat up Time gt 5 C per minute Temperature Controller Digital PID single loop Temperature Display Actual or Set single LED Digital Thermometer Output Thermocouple VII ORDERING INFORMATION A System Components Cat Description UoM 1057 30 0 Hybex Microsample Incubator heating base only 115V Each 1057 30 2 Hybex Microsample Incubator heating base only 220V Each 1057 37 0 Additional Microarray Incubation chamber Complete with slide racks assembly tool and handle Each RP1057 32 1 Replacement absorbent pad for Microarray Incubation Chamber Cover 25 pack Pack RP1057 32 3 Additional Hybex Microarray Rack holds 4 slides Each RP1057 32 5 Additional Handle for duplexed Hybex racks Each RP1057 32 3 Replacement Microarray Incubat
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