User manual
Contents
1. Equipement basse tension N 2006 95 ieee amp lectromagn tique N 2004 108 CEE compatibility D chets d quipement lectrique et lectronique N 2002 96 CEE Waste Electrical and electronic equipment Restncton des substances dangereuses 2011 65 CEE Et sont conformes aux normes Compatibilit amp lectromagn tique NF EN 61326 1 S curit lectrique NF EM 61010 1 Electrical safety NF EN 61010 2 081 S curit laser MF EN 60825 1 Normes aux versions en vigueur lars de la fabrication des scanners Current version standards at the time of scanners manufacturing Sous r serve d installation et d utilisation conformes aux Instructions constructeur An n e du marquage CE 09 Lieu et date d mission Stephane Lebrun a Place and date of issue POG gt lt Y Carbonne France 02 07 2014 C line Hardy Qualit amp Affaires r glementaires Responsable du Dossier technique Quality and Regulatory Affairs c Technical file Manager Ref D ciaration canfarmit Rev 05 1 1 I nnoScan user manual 36 Technical support INNOPSYS 1 Parc d Activit s Activestre 31 390 Carbonne France Phone 33 561 971 974 Email support innopsys fr InnoScan user manual 37 Function Signature Signature num rique de JULIEN JULIEN g DN cn JULIEN CALVE o lnnopsys ou recherche et developpement email j calve inno2. Size Weight Electric consumption InnoScan 1100 AL Specifications 322 x 656 x 439 mm L x P x H 33Kg 100 240 V 220 V 230V 110 V 124V Initialization 45 W 45 W Stand by 40 W 40 W Connected 41 W 41 W Barcode 44 W 44 W reading Scan 45 W 45 W Description Size Weight Electric consumption InnoScan 300 R InnoScan 300 G Specifications 322 x 656 x 439 mm L x P x H 35Kg 100 240 V 220 V 230V 110 V 124V Initialization 46 W 45 W Stand by 43 W 41 W Connected 45 W 45 W Barcode 47 W 45 W reading Scan 48 W 47 W Description Dimensions Poids Puissance InnoScan user manual 344 x 426 x 163 mm L x P x H 11Kg 100 240 V 220 V 230 110 V 124 V I nitialisation 30 W 29 W En attente 20 W 18 W Connect 21W 20 W Lecture CAB 25 W 25 W Lecture lame 25 W 24 W 35 Declaration of conformity INNOPSYS 7 DECLARATION DE CONFORMITE Declaration of Conformity Nous INNOPSYS We Parc Activestre 31390 CARBONNE France D clarons me les produits Declare that the products Scanners de fluorescence de biopuces MicroArray Scanners Modele INNOSCAN 710 INNOSCAN 910 INNOSCAN 710 IR Range IMMOSCAN 710 AL INNOSCAN 910 AL INNOSCAN 710 IR AL INNOSCAN 1100 AL INNOSCAN 300 G Satisfont aux exigences des directives Satisfy the requirements of the directives Machine N 2006 42
3. When the scanner is powered up the LEDs light up as indicated the chapter Meaning of visual and audio signals Moving parts detectors laser power and the Ethernet port are all checked If an error is detected three long beeps sound and the red LED indicating a technical problem lights up If this happens switch off the scanner immediately and contact Technical Support During slide scanning laser power and the autofocus are checked constantly The scanner will report if either of these servo controls did not function correctly as this can produce errors If such errors keep recurring refer to the Troubleshooting chapter with slide check You can also check the scanner more precisely if you so wish 710 710 IR 910 and 1100 AL scanners Insert the Innopsys slide provided with your scanner and run a check from the Scanner gt Check scanner menu in Mapix The fluorescence signal image red green shift 710 910 image red infrared shift with 710 IR or image red green shift red blue shift with 1100 AL and detection gain linearity are all checked For 710 crosstalk is also checked The fluorescence signal and image shift are compared to a reference value and to values obtained when the last check was run A data integrity check is also performed The scanner indicates any malfunction detected In this case contact Technical Support The slide supplied with your scanner is valid for one year The s
4. Y pinhole L Pn focus lens pinhole lens fluorescence from a closer plane best focalisatian plane fluorescence from a farther plane For 710 710 IR 910 and 1100 AL scanners the InnoScan s autofocus system achieves excellent measurement reproducibility irrespective of microscope slide quality plastic slides which are not perfectly flat can be used 300 R and 300 G do not have autofocus fixed focalization but the large depth of field of these systems enables them to compensate imperfect flatness of the slides The XY drive system provides very high sample throughput and makes the InnoScan the fastest scanner on the market up to 35 lines a second while maintaining accurate positioning of the read head thanks to a real time control system For InnoScan 710 and 910 CY3 CY5 Alexa 647 Alexa 660 Alexa 546 and Alexa 555 dyes can be used For InnoScan 710 IR AlexaFluor 700 AlexaFluor 680 Dylight 680 IRDye 680 IRDye700 Dy 680 AlexaFluor 790 Dy 780 IRDye 800 Dylight 800 dyes can be used For Innoscan 1100 AL Cy2 CY3 CY5 Alexa 647 Alexa 660 Alexa 546 Alexa 555 Alexa 488 FITC Sytox orange Drag5 dyes can be used I nnoScan user manual 26 For InnoScan 300 R CY5 Alexa 647 and Alexa 660 can be used For InnoScan 300 G CY3 Alexa 546 and Alexa 555 can be used For InnoScan 710 710 IR and 910 two laser sources are used For InnoScan 710 and 910 one is a wavelength of 635nm for CY5 type che
5. Meaning of icon power on connecting microarray read present I nnoScan user manual 14 Meaning of icon technical problem Information displayed status LED on flashing slowly flashing rapidly beep scanner on powering up switching on power DA end of power up seguence scanner ready to connect short connecting zu connection sequence completed scanner ready to take a slide long disconnecting short slide inserted scanner ready to read reading reading completed slide ready for removal shorts technical problem In the event of a technical problem the scanner must be switched off and Technical Support contacted immediately I nnoScan user manual 15 Operating the scanner Please refer to the software user manual supplied with the device Microscope Slides Only insert ISO 8037 1 compliant microscope slides into the device to the exclusion of any other object These slides are 25 to 26 mm wide 75 to 76 mm long 0 9 to 1 2 mm thick Make certain these slides are not damaged chiped edges for instance Take care about labels sticked on slides They have not to stick up from the slide or to come unstuck These precautions are of the most importance when using an autoloader system in association with InnoScan The edges of the slides should be refl
6. a scanner with wavelength 635nm It is able to scan Slides with 10um pixel size InnoScan300 G a scanner with wavelength 532nm It is able to scan Slides with a 10um pixel size InnoScan710AL is an InnoScan710 scanner associated with 24 slides loader InnoScan710 IR AL is an InnoScan710 IR scanner associated with a 24 slides loader InnoScan910AL is an InnoScan910 scanner associated with a 24 slides loader as well InnoScan 1100 AL with a 24 slides loader has excitation lasers with wavelengths 635nm 532nm and 488nm It is able to scan slides with a 0 5 pixel size I nnoScan user manual 11 Installing the scanner List of packaged components e Scanner mains power cable ethernet crossover cable STP Shielded Twisted Pairs e User manual software installation CD e Software user manual e nnopsys check slide for 710 710 IR 910 and 1100 AL scanners Electrical Communication Connection Before you connect the scanner to your electrical communication please ensure that the wall socket is grounded e your electrical communication follows these specifications Power supply 100 240 V 47 63 Hz e Fuse 4A at 110V at 220V Electrical communication circuit breaker able to switch at least in 40ms Without a grounded socket and or if your electrical circuit does not comply with these specifications the equipment should be damaged and the user s safety should not be ensu
7. must call Technical Support if the machine exhibits any failure or malfunction The housing may only be opened by qualified personnel appointed by Innopsys With 710 710 IR and 910 Innoscan single slide a protective shutter obstructing the slide insertion port closes when the slide is swallowed by the scanner If this shutter remains in the closed position for any reason whatsoever preventing you from inserting a slide or removing a previously Inserted slide do not attempt to force it open call Technical Support immediately Damage to this shutter may expose you to laser hazards With InnoScan equiped with AutoLoader system the user has to close a sliding door after inserting slides in the autoloader system This door will be automatically locked during a scan Do not attempt to force it open during a scan Damage to this sliding door may expose you to laser hazards If for any reason the door is still locked after scanning or if you are able to open the door during a scan immediately switch off the machine and contact Technical Support With 300 R and 300 G InnoScan the user must close the door after inserting the slide If the user opens the door during the reading the laser source is switched off If a slide is broken inside the scanner or cannot be easily extracted do not try and get it by force do not attempt to open the housing but contact Technical Support If the scanner housing is damaged do not use the equipment and conta
8. could be adjustable with the gain The lasers are on when the scanner connects and are off when it disconnects To economize the lifetime of the lasers it is advisable to disconnect the scanner when it is not being used for long periods of time A read with the following parameters lasts 4 minutes e pixel size 10um Speed 35 lines s I nnoScan user manual 27 e read area full slide Yes scan speed is adjustable from 10 lines s to 35lines s Reducing scanning speed provides a better signal to noise ratio which can be useful In applications characterised by low fluorescence Yes pixel size can be adjusted from 10 to 40um with InnoScan 300 and InnoScan 300 G from to 40um with InnoScan710 InnoScan710 IR from 1 to 40um with InnoScan910 and from 0 5 to 40um with InnoScan 1100 AL The memory size of a full Slide image acquired at a pixel size of 5um saved to a tiff file is approximately 256Mb If you reduce pixel size you obtain better resolution but a larger image file and you have to ascertain whether the PC is capable of handling files this big For instance a full slide image at a pixel size of Sum requires at least 2Gb of RAM We use photomultipliers because it provides results with better signal to noise ratios thanks to very low background noise and high sensitivity Yes detection gain may be modified from 0 to 100 The gain variation is linear allowing the user to adjust this setting very precisely It m
9. to 26 C Humidity 20 95 HR Altitude 0 2300 m Description Laser Power PMT Gain Autofocus Detection Dynamique Range Uniformity InnoScan user manual Adjustable 2 laser powers available Adjustable from 0 to 100 Real time autofocus Real confocal and high performances PMT Over than 10 Lower than 5 CV 32 Sample Max Scanning Area Scan Speed Scanning Time Barcode Interface Operating condition All standard microscope slide Width 25 26 mm Length 75 76 mm Thickness 0 9 1 2 mm 22 x 74 mm Adjustable from 20 to 35 lines second max 25 lines second with a 0 5um pixel size 3 5 minutes with a scan area of 22x74mm with a 10um pixel size for a 1 2 or 3 color simultaneous acquisition Codabar code39 linterleaved 2 of 5 code93 code 2 of 5 lata code 2 of 5 matrix 2 of 5 code11 code128 telepen upcA E0 E1 eanJ AN13 J AN8 UCC msi plessey rss 14 limited expanded china post code pdf417 Ethernet Temperature From 19 C to 26 C Humidity 20 95 HR Altitude 0 2300 m InnoScan 710A InnoScan 710AL InnoScan 710 IR InnoScan 710 IR AL Specifications Description Optical resolution 2 7 um 635 nm spot diameter FWHM 3 um 670nm spot diameter FWHM Pixel Resolution From 3 to 40 um InnoScan 910 InnoScan 910AL Specifications Description Optical resolution lt 0 6um 635 532 nm spot diameter FWHM Pi
10. 0 IR AL Specifications 532 nm Cy3 Alexa 546 Alexa 555 532 nm Description Excitation wavelengths Compatible dyes InnoScan 710A 710AL 710 1 670 785 AlexaFluor 700 AlexaFluor 680 Dylight 680 Dy 680 IRDye680 700 670nm Alexa 790 780 IRDye 800 Dylight 800 785nm Description InnoScan user manual 31 InnoScan 710 IR AL InnoScan 300 R InnoScan 300 G Specifications Laser Power PMT Gain Autofocus Detection Dynamique Range Uniformity Sample Max Scanning Area Scan Speed Scanning Time Barcode Interface Operating condition InnoScan 910 InnoScan 910AL Innoscan 1100 AL Specifications Adjustable 2 laser powers available excepted for 300 R which has a fixed laser power Adjustable from 0 to 100 Real time autofocus Real confocal and high performances PMT Over than 107 Lower than 5 CV All standard microscope slide Width 25 26 mm Length 75 76 mm Thickness 0 9 1 2 mm 22 x 74 mm Adjustable from 10 to 35 lines second 3 5 minutes with a scan area of 22x74mm with a 10um pixel size for a 1 or 2 color simultaneous acquisition Codabar code39 linterleaved 2 of 5 code93 code 2 of 5 lata code 2 of 5 matrix 2 of 5 code11 code128 telepen upcA E0 E1 AN13 J AN8 UCC msi plessey rss 14 limited expanded china post code pdf417 Ethernet Temperature From 19 C
11. DANGER Class 3B laser radiation when open AVOID DIRECT EXPOSURE TO BEAM This device complies with 21 CFR 1040 10 amp 1040 11 amp 21 CFR 1010 Attention laser radiation inside Attention dangerous voltage inside Attention read carefully instructions for use Separate collection for the electrical and electronic equipment EPI I nnoScan user manual 2009 03 709001 MODEL IS 710 90021 Manufacturing date VN Serial number MODEL Scanner model 300 R 300 G 710 710 IR 910 1100 AL with or without Autoloader system VN Version number of the instrument Pictograms are associated to each connector to explain its function DIAGNOSTIC RS232 port used for diagnostic issue Ethernet RJ45 port Alternating current Current amp rating of fuse to be replaced InnoScan user manual Safety important information Only qualified personnel who knows understands the safety warnings and precautions described in this document may be authorized to use the InnoScan scanner Use for any purpose other than the purpose indicated in this manual exposes the user to serious hazards Caution use of controls or adjustments or performance of procedures other than those specified herein may result in hazardous radiation exposure The following safety precautions are compulsory and must be stringently observed at all times Laser Safet InnoScan 710 710 IR 910 1100 AL These devices cont
12. Scanners InnoScan User manual PAGE FINDER IDENTIFICATION AND CERTIFICATION LABEL SAFETY IMPORTANT INFORMATION LASER SAFETY HIGH VOLTAGE PARTS SAFETY SYSTEM USE TERMS THE INNOSCAN SERIES INSTALLING THE SCANNER LIST OF PACKAGED COMPONENTS ELECTRICAL COMMUNICATION CONNECTION INSTALLING THE SCANNER SCANNER CHECK AFTER INSTALLING THE SCANNER RESTORING FACTORY SETTINGS MEANING OF VISUAL AND AUDIO SIGNALS OPERATING THE SCANNER USING THE SOFTWARE MICROSCOPE SLIDES OPERATING THE SINGLE SLIDE SCANNER OPERATING THE AUTOLOADER SCANNER SCANNER SELF TESTS AND CHECKS INITIALIZATION CHECKS SCAN CHECKS INNOPSYS SLIDE CHECK MAINTENANCE AND SERVICE TROUBLESHOOTING FAQ TECHNICAL DATA EMBEDDED FIRMWARE SOURCE CODE OF PARTS OF THE INNOSCAN EMBEDDED FIRMWARE SPECIFICATIONS DECLARATION OF CONFORMITY TECHNICAL SUPPORT InnoScan user manual Identification and Certification Label Two labels are affixed at the rear of the InnoScan as show on the picture below UINNOPS YS 000000 XX XXX 000X0 710 710 scanners I nnoScan user manual 3 0000 00 INNIOPS YS 000 XXXXX 00000 300 R and 300 G scanners The following pieces of information are present ININOP SYS 7 2 240 NINI k Made in France 7 100 240 VAC INNOPSYS www innopsys fr 1 2 230 Parc d activit s Activestre 31390 Carbonne FRANCE 50 60 Hz CLASS x LASER PRODUCT
13. ain two laser sources These laser sources are harmless when the equipment is used for its intended purpose In all other cases the laser sources may cause bodily injury gt For 710 and 910 scanners the technical specifications of the laser sources located in the machine are as follows e sourcel visible wavelength 635nm continuous wave maximum output 15mW divergence 1mrad Class 1106 e SOUrce2 visible wavelength 532nm continuous wave maximum output 15mW divergence 1mrad Class IIIb For 710 IR scanners the technical specifications of the laser sources located in the machine are as follows e sourcel visible wavelength 670nm continuous wave maximum output 15mW divergence 1mrad Class IIIb e source2 non visible wavelength 785nm continuous wave maximum output 25mW divergence 1mrad Class IIIb I nnoScan user manual 6 gt For InnoScan 1100 AL scanners the technical specifications of the laser sources located in the machine are as follows e Sourcel visible wavelength 635nm continuous wave maximum output 15mW divergence 1mrad Class IIIb e Source2 visible wavelength 532nm continuous wave maximum output 15mW divergence 1mrad Class 116 e SOUrce3 visible wavelength 488nm continuous wave maximum output 15mW divergence 1mrad Class IIIb InnoScan 300 R and 300 G These devices contain one laser source This laser source is harmless when the equipment is used for its intended purp
14. ay be useful to cancel out Saturation or to equalize channels for instance The scanner features a high precision mechanical positioning system based on a patented real time control loop Absolute positioning is typically over the entire Slide with InnoScan300 R and 300 G 10 um with InnoScan710 InnoScan710 IR on a 22mmx22mm scanned area InnoScan910 InnoScan 1100 AL I nnoScan user manual 28 The scanner is capable of reading all these symbologies e codabar code39 Interleaved 2 of 5 code93 code 2 of 5 lata code 2 of 5 matrix 2 of 5 codell 128 telepen e UpcA EO E1 e eanJAN13 J ANS UCC MSI e plessey rss 14 limited expanded china post code pdf417 I nnoScan user manual Embedded firmware Source code of parts of the InnoScan embedded firmware This is the description for the source code of the parts of InnoScan firmware that fall under open source licenses GNU GPL v2 0 Parts of the firmware under GNU GPL v2 0 license are made available on demand at contact innopsys fr Innopsys makes no warranties whatsoever either express or implied including any warranties of merchantability or fitness for a particular purpose regarding this software Innopsys offers no support for this software The preceding does not affect your warranties and statutory rights regarding any Innopsys product s you purchased It only applies t
15. ct Tecfnical Support For 710 710 IR 910 and 1100 AL if any laser light is visible for any reason whatsoever immediately switch off the machine and contact Technical Support For 300 R and 300 G scanner if the laser remains switch on when the door is opened immediately switch off the machine and contact Technical Support Warning replace the fuse by a fuse of the same type e 2AT 250V e Type 5 20 mm e Breaking capacity 150A Instructions to replace the fuse e Disconnect power cable and switch off the scanner e Turn 5 5 mm plat screwdriver from right to left inside the fuse holder to remove it e Replace the fuse and move back the fuse holder on the rear side of the scanner InnoScan user manual 8 e Turn the screwdriver from left to right inside the fuse holder to lock it InnoScan user manual Use terms The scanner is designed for use in research only It is not designed or certified for use in the diagnosis of human or animal diseases InnoScan user manual 10 The InnoScan series The range Innoscan includes nine different models InnoScan710 has excitation lasers with wavelengths 635nm and 532nm It is able to scan slides with a um pixel size InnoScan710 IR has excitation lasers with wavelengths 670nm and 785nm is able to scan slides with a um pixel size InnoScan910A has excitation lasers with wavelengths 635nm 532nm It is able to scan slides with a 1 um pixel size InnoScan300 R
16. e the new filter and then interlock the protection grid by pressing each side No other user maintenance is required No other user service is allowed Ifa problem occured please contact your distributor InnoScan user manual 24 Troubleshooting Sympton Diagnosis The image is dark or the measured signal is weak Make sure the detector gain is not too low set to 100 to check If laser power is adjustable make sure the laser power settings are not too low set to high to check Make sure the slide fluorescence marking is compatible with InnoScan see list of supported fluorophores in FAQ Technical Data Check the scanner usi see Scanner self tests and checks For scanners 710 710 IR and 910 and 1100 AL the image is striped or Mapix displays a Focus was not stable during the scan message The autofocus may not work properly with certain types of slide It needs a clean reflective surface A large bar code may cause problems You can set the bar code height in Mapix see your Mapix manual Certain types of slide e g with black edges may also cause problems You can select the slide type in Mapix see your Mapix manual Thick spots deposited around the edges of the scan area may also confuse the autofocus system Contact Technical Support to try to resolve the problem Otherwise you can deactivate the autofocus The image is distorted and Shapes are the wrong size See previ
17. ective to be detected by the autoloader system Verify that there is no safety film on the slide or any other sticky material The slide could not be removed from the scanner otherwise The scan area is shown below The slide is inserted in the direction indicated by the arrow 185mm for InnoScan910 or InnoSCan910AL I nnoScan user manual 16 If spots are deposited outside the scan area they could be damaged by the slide gripping system The scanner is easy to operate The only direct user actions required without using the software are e powering up switching off the scanner using the on off button located on the instrument rear panel InnoScan 710 Scanner 910 I nnoScan user manual 17 Scanners 300 R et 300 G When the scanner is powered up it performs a sequence of self tests and will Indicate any malfunction via the Technical Problem LED The test sequence lasts for approximately 30 seconds When the sequence has completed a short beep is emitted If the Technical Problem LED is off the scanner is ready for use It is nevertheless advisable to wait 10 minutes after the scanner has connected as opposed to powered up before use so that the lasers have time to achieve thermal stability For 710 710 IR and 910 scanners placing a microscope slide in the insertion port located on the instrument front panel For 300 R 300 G scanners open the front door and insert the slide Cl
18. he scanner has connected as opposed to powered up before use so that the lasers have time to achieve thermal stability e Slid down the door until full opening Insert slides horizontally in the loader slots numbered from 1 to 24 Gently push them until they reach the stop I nnoScan user manual 20 T d p The barcode and microarray spots must be on the same face of the slide i e upwards as shown in the previous illustration Gently slide up the door to close the autoloader system The microarray presence detector detects that a slide has been inserted and the microarray present LED comes on indicating that the scanner is ready to read the sample During a scan the door is locked Do not attempt to force it open you may harm the scanner Wait for the end of the scans see chapter Meaning of visual and audio signals before sliding down the door and getting back the slides Any other use of the scanner may be dangerous or may harm the scanner Do not attempt to loosen screws or insert any object other than slides Be sure that inserted slides complied with ISO standard 8037 1 see chapter Microscope Slides Be sure that inserted slides are pushed until the end stop Be sure that inserted slides are horizontal and not inserted on two different levels InnoScan user manual 21 Scanner self tests and checks The scanner runs three levels of self tests and checks Initialization lt
19. mifluorescent dyes and the second is a wavelength of 532nm for CY3 type chemifluorescent dyes For InnoScan 710 IR one is a wavelength of 670nm for AlexaFluor700 type chemifluorescent dyes and the second is a wavelength of 785nm for AlexaFluor790 type chemifluorescent dyes For InnoScan 1100 AL three laser sources are used One is a wavelength of 635nm for CY5 type chemifluorescent dyes the second is a wavelength of 532nm for CY3 type chemifluorescent dyes and the third is a wavelength of 488nm for CY2 type For InnoScan 300 R 300 one laser source is used For InnoScan 300 the wavelength used is 635nm for CY5 type chemifluorescent dyes For InnoScan 300 the wavelength used is 532nm for CY3 type chemifluorescent dyes The laser sources are temperature and current controlled to provide an extremely stable power output Power fluctuation does not exceed 2 of power output To achieve the most accurate results you are advised to wait 10 minutes after the scanner has connected The lasers will have reached their stable nominal operating temperature by then Yes for InnoScan 710 710 IR 910 1100 AL and 300 the lasers power can be set at two different levels high and low Low power is useful to decrease the bleaching of the fluorophores and to increase the lifetime of lasers High power is used to obtain the best sensitivity results when it is a critical point InnoScan 300 R has only one laser power the intensity
20. o this source code made available to you Every software listed below is under GNU GPL V2 0 open source license which Is available at the following address http www qgnu org licenses gqgpl 2 0 html U boot 1 2 0 and modifications This software is used to initialise and to launch the system noyau linux DENX ELDK based on kernel 2 4 25 and modifications This software is the kernel of the linux embedded operating system busybox 1 2 1 This software provides a fairly complete environment for any small or embedded system boa 0 94 14 0 5 21 This software is a http server eraseall 0 1 0 This software is used to erase flash memory I nnoScan user manual 30 Specifications InnoScan 710A InnoScan 710AL InnoScan 910A InnoScan 910AL Specifications Description Excitation wavelengths Compatible dyes InnoScan 1100 AL Specifications 635 nm and 532 nm Cy5 Alexa 647 Alexa 660 635 nm Cy3 Alexa 546 Alexa 555 532 nm Description Excitation wavelengths Compatible dyes InnoScan 300 R Specifications 635 nm 532 nm et 488nm Cy5 Alexa 647 Alexa 660 Draq5 635 nm Cy3 Alexa 546 Alexa 555 Sytox orange 532 nm Cy2 Alexa 488 FITC 488nm Description Excitation wavelength Compatible dyes InnoScan 300 G Specifications 635 nm Cy5 Alexa 647 Alexa 660 635 nm Description Excitation wavelength Compatible dyes I nnoScan 710 IR InnoScan 71
21. oftware will no longer allow you to run checks with it beyond this date and you must procure a new one The slide must be stored in its box at room temperature and away from light when not being used You are advised to check the scanner once a month or each time you move it Only use this slide with the scanner it was delivered with InnoScan user manual 22 300 R and 300 G scanners For these scanners the check slide is already included in the scanner The user runs a check directly from the Scanner gt Check scanner menu The fluorescence signal and detection gain linearity are checked The fluorescence signal is compared to a reference value and to values obtained when the last check was run A data integrity check is also performed The scanner indicates any malfunction detected In this case contact Technical Support The slide included in the scanner is valid for 3 years Beyond this date the slide has to be changed InnoScan user manual 23 Maintenance and Service Fan filter replacement For models equipped with a fan filter it is necessary to replace the filter by a new one every year In the event of failure to meet the deadline for replacement of the filter the scanner warranty deed should be revoked Customer has to order a new filter to its distributor To replace the filter see picture remove the protection grid which is interlocked at the back of the scanner remove the old filter plac
22. ose In all other cases the laser source may cause bodily injury For 300 R scanner the laser source located in the machine has the following specifications visible wavelength 635nm continuous wave maximum output 15mW divergence 1mrad Class IIIb For 300 G scanner the laser source located in the machine has the following specifications visible wavelength 532nm continuous wave maximum output 15mW divergence 1mrad Class IIIb For all types of scanners do not attempt to gain access to the interior of the device Some electronic assemblies in the device require high voltages gt 1000V to operate normally These sources are harmless when the equipment is used for its intended purpose In all other cases high voltage may cause severe or fatal bodily injury Do not attempt to gain access to the interior of the device Because the InnoScan is a scanner it contains moving parts These parts are harmless when the equipment is used for its intended purpose In all other cases these parts may cause bodily injury Do not attempt to gain access to the interior of the device The InnoScan has been designed to reduce all the above risks to zero when the equipment is used for its intended purpose In the case of Innoscan single I nnoScan user manual 7 slide the device housing and its mechanical shutter system protects you from all danger Never attempt to open the housing No user serviceable parts inside The user
23. ose the door before starting the scan I nnoScan user manual 18 Scanners 300 R et 300 G The barcode and microarray spots must be on the same face of the slide i e upwards as shown in the previous illustration Two rails guide the slide by gripping it by the edges The insertion mechanism is designed not to damage the slide or the array spots deposited on it Gently push the slide home The microarray presence detector detects that a slide has been inserted and the microarray present LED comes on indicating that the scanner is ready to read the sample Any other use of the scanner may be dangerous or may harm the scanner Do not attempt to loosen screws or insert any object other than slides complying with ISO standard 8037 1 Operatinq the autoloader scanner The scanner is easy to operate The only direct user actions required without using the software are e powering up switching off the scanner using the on off button located on the instrument rear panel InnoScan user manual 19 Scanner 710AL Scanners Innoscan 910AL InnoScan 1100 AL When the scanner is powered up it performs a sequence of self tests and will Indicate any malfunction via the Technical Problem LED The test sequence lasts for approximately 30 seconds When the sequence has completed a short beep is emitted If the Technical Problem LED is off the scanner is ready for use It is nevertheless advisable to wait 10 minutes after t
24. ous problem in the case of 710 710 IR 910 and 1100 AL scanners Check if the same problem occurs with all types of Slides Check that the problem isn t being caused by the direction in which the slide is inserted by turning it around 180 and then reinserting it If the defects are also rotated 180 the slide is causing the problem Otherwise contact Customer Support There is a spot in the same place on all images whatever slide you use Contact Customer Support Avoid using slides with liquids likely to run during scanning I nnoScan user manual 25 FAQ Technical data The InnoScan is a confocal fluorescence scanner For 710 710 IR and 910 scanners the imaging system consists of two laser sources to excite the chemifluorescent dyes 635nm and 532nm for InnoScan 710 and 910 670nm 785nm for InnoScan IR two detection photomultipliers For InnoScan 1100 AL scanners the imaging system consists of three laser sources 635nm 532nm and 488nm and three detection photomultipliers A very high precision optical filters provide results with enhanced signal to noise ratios 300 R and 300 G scanners consist of one laser source 635nm for 300 R and 532 for 300 G and one detection photomultiplier Confocal means that fluorescence emitted from planes other than the plane on which the scanner s optical system is focussed is not measured thus cancelling out all parasite fluorescence photodetector
25. psys fr c FR Written by R amp D department Date 2014 07 03 14 34 03 02 00 Celine Hardy cn Celine Hardy o Innopsys ou Responsable Qualite et affaires reglementaires email c hardy innopsys fr c FR 2014 07 03 17 15 13 02 00 Approved by Quality manager I nnoScan user manual 38 NNOPSYS www innopsys fr Originale instruction version 3 4 1 InnoScan user manual 39
26. red Installing the scanner The scanner must be located on a base not subject to strong vibrations centrifuge vortex etc not exposed to direct sunlight that prevents the housing from coming into any contact with chemicals especially acetone e Block scanner ventilation allow 5 cm of space from the rear panel of the scanner Move the scanner while it is functioning Remove any slide from inside of the scanner before moving it If necessary ask for colleague assistance to move the scanner Put the scanner on a table strong enough to bear the weight of the scanner I nnoScan user manual 12 Connect the scanner to a grounded mains outlet 110V or 220V by using the mains power cable supplied Connect the scanner to a dedicated PC via the crossover ethernet cable supplied or to the network via a STP Shielded Twisted Pairs straight ethernet cable Change the IP address and the various network parameters of the scanner as required for your network ask your network administrator for advice To do this enter the scanner s default address 192 168 0 254 in your web browser s navigation bar This will connect you to the machine s web server INNCOPSYS Welcome Network About You re on the embedded web site of an Innoscan machine Thanks to it you can change network parameters Click on Network you may now edit the machine s network parameters NNOPSYS y Welcome M Net
27. work E ETE About Host Name innoscan Domain Name IP Address 192 lhes Gateway 192 158 Netmask 255 255 Broadcast 255 255 Serveur 1 Name Serveur 2 Validate the configuration To confirm your parameters click on Validate the configuration Press Reset to cancel changes To configure the scanner as a DHCP client type 0 0 0 0 as IP Address Scanner check after installing the scanner InnoScan 710 710 910 and 1100 AL Insert the delivered check slide I nnoScan user manual 13 Run Mapix software Click on the lt check scanner gt button in the lt Scanner gt menu InnoScan 300 R and 300 G For these scanners the check slide is included in the scanner Run Mapix software Click on the lt check scanner gt button in the lt Scanner gt menu Mapix displays the checked points and if the scanner is functionnal Otherwise please contact your distributor Restoring factory settings If for any reason the IP address is badly configured or forgotten you can restore the machine s factory IP settings by holding down the reset button as shown in the illustration below while the scanner is powered up L of visual and audio signals 4 LED pictogrammes and a buzzer keep the user informed about the scanner s status at all times See below for the meaning of pictogrammes
28. xel Resolution From 1 to 40 um Sp cifications InnoScan 1100 AL Description Optical resolution lt 0 6um 635 532 488 nm spot diameter FWHM Pixel Resolution From 0 5 to 40 um InnoScan 300 R InnoScan 300 G Specifications Description InnoScan user manual 33 Optical resolution 10 um 532nm spot diameter FWHM Pixel Resolution From 10 to 40 um InnoScan 710A nnoScan 710 IR Specifications Desc ription Size Weight Electric consumption I nnoScan 910 Specifications 278 x 457 x 369 mm L x P x H 15 5 Kg 100 240 V 220V 110 V Initialization 41 W 30 W Stand by 30 W 20 W Connected 32 W 24 W Barcode 35 W 26 W reading Scan 33 W 25 W Description Size 316 x 549 x 432 mm L x P x H Weight 28 Kg Electric consumption 100 240 V 220 V 230 V 110 V 124 V Initialisation 42 W 42 W En attente 43 W 37 W Connect 36 W 38 W Lecture CAB 39 W 38 W Lecture lame 39 W 40 W InnoScan 710AL InnoScan 710 IR AL Description Specifications Size 333 x 595 x 422 mm L x P x H Weight 27Kg Electric consumption 100 240 V 220 V 110 V Initialization 37 W 20 W Stand by 40 W 31 W Connected 35 W 27 W Barcode 35 W 26 W reading Scan 33 W 25 W InnoScan user manual 34 InnoScan 910AL Specifications Desc ription
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