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1. 1 Message fie Program on block 1 manually stopped 26 09 08 Loops b In the sheet Temperature Data each second data for the programmed temperature the block temperature and lid temperature are recorded c The sheet Temperature Curve gives a graphical presentation of the recorded temperature data User Manual TProfessional Manager Software January 2009 43 Biometra An Analytik Jena Company Tomperature Curve of Progra ABO Hemera ENT OT 3 11302 RA aa H EIS anana No H M SS JURON IANI Sa ill AANE AALi By the function Zoom the display of the temperature curves can be optimized Click on Zoom a new window opens Change the settings of specific parameters to optimize the display Min Temperature 20 Max Temperature C Major Unit C M Show Lines Minor Uniti T M Show Lines Automatically Set Time Scale Min Time Ohh mm ss 00 00 00 Max Time hh mm ss 00 00 01 Major Unit hh mm ss 00 00 00 T Stem ince Minor Unit hh mm ss 00 00 00 E Cancel OK 44 User manual TProfessional Manager Software January 2009 10 Subject Index Biometra Shared directory 40 COM ports Installation 11 Configuration Menu 9 Connection disconnect 13 establish 12 Cycler Manager 9 Cycler View 10 Detailed Log File 20 Directory copy 26 open 24 Display menu 9 Error code 782 38 Fehlerbehebung Cycler on COM Port not available 38 File Menu 8 Help Menu 9 Image Fil
2. An Analytik Jena Company I Gerate Manager Anschl sse COM und LPT F ECP Druckeranschluss LPT1 Kommunikationsanschluss COM1 i JF Prolific USB to Serial Comm Port COM12 JF Prolific USB to Serial Comm Port COM13 I Prolific USB to Serial Comm Port COM1 4 oY Prolific USB o Serial Comm Port COM15 aF Prolific USB o Serial Comm Port COM16 3 Prolific USB to Serial Comm Port COM17 i JF Prolific USB to Serial Comm Port COM18 l 3 Prolific USB to Serial Comm Port COM19 H D Audio ideo und Gamecontroller H Computer 4 Diskettencontroller H A Diskettenlaufwerke S DYD CD ROM Laufwerke H Grafikkarte H E IDE ATAYATAPL Controller mre will cele oe le Ta SOM ela tret rel If an external serial hub is used the newly installed serial ports are assigned as USB to Serial Comm Port by the system Make a note of the numbering of the installed serial ports and activate the corresponding ports in the setup menu of the TProfessional Manager Software see chapter 5 1 Note The TProfessional Manager Software can address up to COM port 16 User Manual TProfessional Manager Software January 2009 37 Biometra An Analytik Jena Company 7 2 Error code 782 While connecting TProfessional Thermocycler to the computer you may get a message with error code 782 Cycler didn t respond Repeat procedure 782 eem J Boc Program Cycler didn t answer repeat procedure 782 This messa
3. Remaining Time D Display with connected Thermocycler COM 1 Cycler TProfessional Block Gradient 96 User 44 Remaining Time Status Block free F Login SE Save Detailed Logfile 1 COM Nr number of the addressed serial port 2 Cycler instrument type 3 Block block type used on the Thermocycler 4 User logged in user 5 Status a nothing displayed no Thermocycler connected b Block free c Block active remote control 6 Remaining Time Thermocycler free Thermocycler active program runs remaining time to the end of a run User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 5 Addressing TProfessional Thermocyclers 5 1 Initial installation of COM ports After the network has been installed and the TProfessional Manager was started for the first time there is no Thermocycler addressed by the software In the Cycler Manager window the status message not connected is displayed see chapter 4 2 and in the Cycler View window the status view bar of the serial port contains no entries See chapter 4 3 picture C Before Thermocyclers can be addressed by the PC first the configuration of COM ports has to be defined in the software Open the setup menu in the TProfessional Manager menu bar see chapter 4 1 2 The TProfessional Manager Software automatically starts to read out the available serial ports A new window opens were the serial ports ad
4. AA SESSRERRRERERRRRRAER Remaining Time 2h 13m Estimated End 15 58 Lid 98 9 c COM 4 Fom RICK Show Grad Cycler TProfessional Standard Block 96 well Status Block active Remote control User Aa SESRSRRREREREREEEERR Remaining Time 2h 15m Status connected COM 5 Cycler TProfessional Basic Gradient COM 5 V Block Grad 96 Basic Cycler TProfessional Basic Gradient User AA Block Grad 96 Basic Status Block active Remote control User AA SRSSESSRRRERRRSRERER Remaining Time 2h 29m COM 7 iv Save Detailed Logfile Cycler TProfessional Basic Block 96 well Basic Status starting program Remote control User AA SRSSSSSSARRRERRAARAR Remaining Time 2h Sim 4 1 TProfessional Manager Menu Bar The menu bar of the TProfessional Manager offers four buttons e File e Setup e Configuration e Display e Help The buttons can be activated by a single mouse click whereupon the menu items will be displayed 4 1 1 File Menu The file menu contains the menu item Exit Clicking on Exit stops the TProfessional Manager and the window closes 4 1 2 Setup Menu Activating the setup menu automatically opens a new window where the configuration of serial ports can be defined Chapter 5 1 8 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 4 1 3 Configuration Menu By clicking on the setup menu button a message window opens When activating the check box
5. COM 3 M D Save Detailed Logfile Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Block free User 44 Remaining Time Note You have only access to directories on Thermocyclers that are connected to the computer and addressed by the Manager Software User Manual TProfessional Manager Software January 2009 25 Biometra An Analytik Jena Company Click on the button Program in the menu bar of the TProfessional Manager user interface Click on Open Cycler Directory to open a directory on a Thermocycler Select directory C PP Service Pr director Program directory 6 7 Copy Directories The TProfessional Manager Software allows to copy user directories saved on the PC or saved on the TProfessional Thermocyclers 6 7 1 Copy directories from Cycler to PC Select the TProfessional Thermocycler from where the directory shall be copied in the Cycler View window The field for the actively addressed Thermocycler appears with a white background COM 3 M D Save Detailed Logfile Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Block free User 44 Remaining Time Note You can only copy directories from Thermocyclers to the computer that are connected to the computer and addressed by the Manager Software Click on the menu button System in the menu bar of the TProfessional Manager user interface From the systems menu select item 1 User Configuration and activate subit
6. lt GManGe supervisor PIN sebiesassos sksedea savigskniebsntansslssstnussb rss nisdtnisknss nns its es 32 610 3 Greate Image File eassa eee eee nee een meee ec ee eee SANNE 33 60104 Den Image TOC YCIST arrear EEE a EO ROE A 34 7 Troubleshooting QUIiCC sssssssssssssssarsssrssnnrssnnnnnnnnnn nn ARR ARR ARAA ARA ARR ARA ARR ARR AA RR AA RR RA RR RA RR RAR 36 7 1 Check configuration of serial ports at the computer ssnsssrssssrrsrrrsrrrrsrrrerrrrrerrrrn rr rr nr r nana 36 7 2 ENO COS JOZ tates ease Ge orca cea reead nt caer tea areas doe s eid bsr E Rs 38 7 3 Message Cycler on COM Port not available eisesnsmssessrressrrrsrrrrrrrrrrrrrrrrrrnn rr rr nn rann 38 User manual TProfessional Manager Software January 2009 3 Biometra An Analytik Jena Company 8 Biometra Shared directory sicsisceescicdceicarescndticesdieencesciexaciseeasstesasiecuduersigosseseseusdessecdotadeanten 40 8 1 Transfer of the complete directory including all ProgramMS cccceecceeeeeeeeeeeeeeeeees 40 8 2 Transfer of selected programs ccccsecccesceceececceeecceeecceueecsueeecueeececessueessaeeseeeesnaees 41 9 Analysis of Detailed Log Files ssssssssssssssssrssassrsnnarnnn nnnnannn nn RARE RA ARR RA RENA ARR RARE R ARR RA RENA RR RR RA RR nn 42 10 SUD OCU sss eset cs ccssetenccneen E a a E EEEa 45 4 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 2 Introduction For the TProfession
7. programs if necessary will be adapted to the corresponding block type or cycler type 6 8 Create Service Info File Service Info File contains useful technical data for remote diagnosis and can be sent for readout to the Biometra Service Department To create a Service Info File click on the menu button System in the menu bar of the TProfessional Manger user interface Form the systems menu select item 4 Service and activate subitem 4 4 Create Service Info File 4 Service select topic 41 Poweron selftest logfile 42 Extended block selftest logfiles 43 Error Logfiles 44 Create Service Info File User Manual TProfessional Manager Software January 2009 29 Biometra An Analytik Jena Company The following message will be displayed in the TProfessional Manager user interface 44 Create service into file For diagnosis by Biometra Service create service info file Press Ok enter file name and wait till process ends Email service file to Biometra By activating the check box SINF in the status view of the Cycler View window you can select for which Thermocycler a Service Info File will be saved COM 5 fF SINF IS Save Detailed Logfile Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Block free User 44 os Remaining Time To conform your selection click on OK in the TProfessional Manager user interface Subsequently the Save as windows opens Enter a
8. Directories Be aware to choose the appropriate directory corresponding to the TProfessional Thermocycler the directory will be installed to 3 directory Basic 20081209 dix directory Basic Gradient_ 0081209 dix directory Standard 60 96 384 20061209 dix verwendete D directory Standard Grad 60 20081209 dix E directory Standard Gradient96_TProfessional_ 20061209 dix Desktop Eigene Dateien Arbeitsplatz 4o NERE Dateiname di Dateityp Abbrechen TProfessional Basic directory_Basic_20081209 dix b TProfessional Basic Gradient directory_Basic Gradient_20081209 dix TProfessional amp TProfessional Standard directory_Standard 60 96 384 20081209 dix d TProfessional amp TProfessional Standard Gradient 60 directory Standard Grad 60 20081209 dix e TProfessional amp TProfessional Standard Gradient 96 directory Standard Gradient96 TProfessional 20081209 dix Note The programs of the selected directory are copied from the PC to the Thermocycler directory of the user who is currently logged in to the instrument At first all programs in the 40 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company directory are deleted and then the protocols of the selected PC directory are transferred Please make sure that you are not logged in to a user directory that must not be overwritten 8 2 Transfer of selected programs At the Thermocycler log in the user
9. components are necessary 1 Up to 5 TProfessional Thermocyclers 2 Upto 5 serial null modem cable with mode full handshaking max length up to 15 m 3 Up to 5 serial ports COM ports 4 PC with Windows XP Service Pack 2 SP2 or Vista operating system 512 MB RAM 5 TProfessional Manager Software 3 3 Connecting TProfessional Thermocyclers to the PC At first the connection of the TProfessional Thermocylers to the PC has to be established via a serial null modem cable with mode full handshaking Attach the serial null modem cable to the RS232 serial port at the backside of the TProfessional Thermocycler Note Biometra offers null modem cables with mode full handshaking Ord No 050 821 At second the connection to the PC is established by plugging the cable into the serial port at the computer Note In dependence on the number of TProfessional Thermocyclers to be connected an upgrade of the number of serial ports at the computer may be necessary For this purpose internal PCI serial cards or external serial hubs may be used recommended by Biometra Biometra offers internal PCI serial cards with 4 ports or 8 ports Ord No 050 822 050 823 The next pictures show possible configurations of a network with 5 TProfessional Thermocyclers by connection via an internal PCI serial card A or an external serial hub B A Configuration of a network with internal PCI serial card T mm mT Serial null modem
10. files will be copied to the computer 8 Press Install to start the installation 9 Copy the file TProfManager license in the folder where you have installed TProfessional Manager By standard the folder for the installation IS C Programs Biometra T ProfessionalManager O NOD O18 W PO Note For unrestricted use the TProfessional Manager Software has to be activated by a license Without a valid license the program can be opened and tested up to 20 times User manual TProfessional Manager Software January 2009 7 Biometra An Analytik Jena Company 4 The TProfessional Manager Operating Interface The operating interface of the TProfessional Manager is divided into three areas The menu bar 1 at the top of the screen offers 4 different buttons In the Cycler Manager window 2 on the left a programming interface and a status view for the actually addressed Thermocycler are displayed In the Cycler View 3 window on the right the available serial ports and the connected TProfessional Thermocyclers are listed TProfessional Manager Monitoring Cyclers1 File Setup Configuration View Help ro Cycler Manager Cycler View Select Al Cycler TPrafessional A Program Block Gradient 60 Status Block active Remote control GGL 5 01 standard User AA KEKNNNEENENENERENENER Remaining Time 2h 15m 06 Steps COM 2 iv S Cycler TProfessional Block Gradient 96 Status Block active block was changed Remote control User
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12. on each connected TProfessional Thermocycler 6 5 1 Save Program on PC Click on Save on PC Save program Select program number T LCJ AB Ab 1 Prograrns biometra 02 free 03 free O4 free 05 free 06 free Save on Cycler The dialog box save as opens 22 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company Save ihi B PROGRAMS My Recent Documents Desktop My Documents My Computer My Network File name les Places Save as type Thermacycler Pragram Files tcp Cancel Enter a name for the program in the field file name Press the button Save to save the program 6 5 2 Save Program on Thermocyclers Click on Save on Cycler Save program Select program number biometa 02 free 03 free 04 free 05 free 06 free Save on Cycler lt appears a notification that in the Cycler View window it can be defined at which connected TProfessional Thermocyclers the program will be saved User Manual TProfessional Manager Software January 2009 23 Biometra An Analytik Jena Company Copy program AA 06 standard to To use multiple cycler Control check boxes in Cycler View window By activating deactivating the check box Save in the status view of the Cycler View window you can select at which Thermocyclers the program will be saved By routine the check boxes of all available TProfessional Thermocy
13. serial ports by clicking on the Connect button in the connect Disconnect window of the Cycler View screen The network is installed now and all Thermocyclers can be addressed 38 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company User Manual TProfessional Manager Software January 2009 39 Biometra An Analytik Jena Company 8 Biometra Shared directory Since January 2009 all TProfessional Thermocyclers are delivered with preinstalled PCR protocols These are located in the directory Biometra Shared If you would like to transfer the sample protocols to instruments delivered before this date you can use the T Professional Manager Software Either the complete directory including all programs or selected programs can be transferred presupposed during the setup of the TProfessional Manager Software the directories respectively the programs have been installed to the computer see chapter Fehler Verweisquelle konnte nicht gefunden werden 8 1 Transfer of the complete directory including all programs At the Thermocycler create a new user directory Biometra Shared and log in to this directory Transfer the data to the instrument by using the function Copy directory from PC to Cycler see chapter Fehler Verweisquelle konnte nicht gefunden werden Note The standard installation folder of the Biometra Shared directories is C Programs Biometra T ProfessionalManager BiometraShared
14. Biometra An Analytik Jena Company Professional Manager Software User Manual January 2009 Biometra GmbH Service Department Rudolf Wissell Str 30 Rudolf Wissell Strasse 14 16 D 37079 G ttingen D 37079 G ttingen Tel 49 551 50686 0 Tel 49 551 50881 10 12 Fax 49 551 50686 66 Fax 49 551 50881 11 email info biometra com email service biometra com Internet http www biometra com Biometra An Analytik Jena Company User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 1 Table of contents g Tabe o gece 9 gt 9 eee ee ee ee eee 3 2 A142 2 0 e Ul 2 9 Sahin EE ene eae ee eee eee eee ene 5 2 1 TProtessional Manager sccsstrsss stirs cones secetsdacepdacenanssauydedaaateauneysetsrasscouatannumoass SNORRES 5 3 Installation Of the NETWOFK mssssssssssssssssssssssrsrnn rn rss rn rn nnn nn An RR RAR RR ERA RAR RAR RR ERNER RR RAKA RAR R REAR RR RR RR RR RR R 6 3 1 GONIENT OF GOI CNY sgsstisossiueiotsedensorennsnssutosssorsbesislitnsigsd ONS 6 3 2 ESSSNNANG OMP ODES 0 oan ne en ene ee E 6 30 Connecting TProfessional Thermocyclers to the PC ccccccceseeceseeeseeeeseeeeseeeeeees 6 3 4 Installation of the TProfessional Manager Software ccccccsececeeeeceeeeeeeeseeeeeseeeees 7 4 The TProfessional Manager Operating Interface ccccsssseeeeeseeeeeneeseenseeeenneesennees 8 4 1 TProfessional Manager Menu Bat ccccssccceeeccesee
15. Save automatically Run Logfiles will be stored automatically in a preset folder for each run Configuration Save each Run Logfile automatically on PC after a run Cancel Save automatically Run Logfile Folder is C Documents and Settings All Users Documents BIOMETRA TProfManager RUM LOGFILES 4 1 4 Display Menu In the display menu the user can switch between the two windows Cycler Manager and Cycler View In each case the active displayed window is tagged by a check mark in the menu 4 1 5 Help Menu The help menu offers information on the software Manager Info or useful hints for the use of the TProfessional Manager Tips and Tricks 4 2 Cycler Manager Window In the Cycler Manager window a user interface like on the TProfessional Thermocyclers is integrated Menu buttons Connect to cycler or edit existing programs Work on PC You can write or edit programs on PC Please press softkey New program or Open PC program To start them you have connect to cycler Work on cycler You can write or edit programs start them and record logfiles on PC To work on cycler press button Connect Disconnect in cycler view window fer Open PE pa pT en ERE Context sensitive menu buttons The menu navigation functions as it is described in the manuals of the TProfessional line In contrast to the Thermocyclers the four buttons Login out System Block and Program in the u
16. T Professional Manager Software Connect the adapter to USB port that has been used at first The numbering of serial ports is different between single computers und single USB ports The Setup menu of the TProfessional Manager Software offers only displays active COM ports and therefore offers some help to select the appropriate serial ports see also chapter 5 1 Serial ports that are not installed at the computer appear greyed out and are not selectable To check the configuration of the serial ports at the computer activate the menu item lt System control gt under lt Settings gt in the Windows start menu The window System Properties opens System Properties Spain Ambag Dirag D mgpuii Hara Deyme h rus The Derme Mars bhiir a the hadat daroe metalied pn on pour computa Woe he Device Mares bo changa he SE properties of ary descr Di pest S kneg les pt von ound Ia erties doser ae etta wath vand Wicki Update belt pou bet up Far Wmi corrects bo yikes Update lon dyrt o Dieii L inw Update Hagha Pree Hadasa poiss provide 5 vs f r d bo set op d shoe el diet handen conigueahond Activate the register lt Hardware gt and click on the button lt Device Manager gt The new window Device Manager opens At lt Ports COM amp LPT gt you get a list with the numbering of the serial ports installed by the Windows system 36 User manual TProfessional Manager Software January 2009 Biometra
17. Version Blocktype Block Serial No Block No Messages Time Message To open a Detailed Log File click on open Logfile and choose the appropriate file Note Detailed Log Files are saved to a user specific folder by the computer The files are stored to the directory My Documents of the user that is currently logged in to Windows The files are located in the following folder My Documents BIOMETRA TProfManager LOGFILES DETAILED LOGFILES The Excel file is divided into three sheets a Logfile Data b Temperature Data c Temperature Curve a The sheet Logfile Data displays Log File data messages that have been recorded during the run and the used PCR program 42 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company Detail_Logfile 2908141_ AA_1_20080926_11h39m txt Run Logfile Printing Time and Date Open Run Logfile of Program Logfile Log Time and Date Start End Time Program User Cyclertype Company Serial No Software Version Blocktype Block Serial No Block Ho Messages Time Date 11 44 26 09 08 Temperature Program View program 44 01 testlagfile Blocktype 46 well Preheat Lid ON 99 C Steps a0 biometra Part of GE Healthcare Clear 11 40 26 09 08 AA 01 testlogtile 11 44 26 09 08 11 44 11 44 26 09 08 AA Pro User TProfessional Standard Biometra 2408141 0330 1 30 1 30 1 20tpr 96 well 20081568
18. al Manager Software Biometra pursues the idea to provide a software that allows to control TProfessional thermocyclers in a powerful network To achieve this goal an efficient control software has been developed and the concept for programming thermocyclers of the TProfessional line was integrated The result is an easy to use software with intuitive operating interface as it is known from the TProfessional Thermocyclers themselves 2 1 TProfessional Manager The Biometra TProfessional Manager is a PC Software that allows to install and to control a network of up to 5 Thermocyclers of the TProfessional series With the Manager Software programs can be created edited or saved and user directories and programs can be exchanged between the PC and the TProfessional Thermocyclers The handling of the TProfessional Manager is based on the intuitive user interface of the Thermocyclers with the choice for tabular or graphical programming For documentation of PCR runs detailed log files with temperature data can be saved If problems occur in an extended self test Service Info Files are created that can be analysed by Biometra s Service for rapid failure diagnosis User manual TProfessional Manager Software January 2009 5 Biometra An Analytik Jena Company 3 Installation of the network 3 1 Content of delivery 1 TProfessional Manager Software and license file 2 Manual 3 2 Essential Components For the installation of the network the following
19. cables LJ pc 6 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company B Configuration of a network with external serial hub AE Serial null modem cables External serial hub USB cable O PC Note The ports of an external serial hub are usually numbered e g P1 to P8 The numbering of the ports installed in the Windows system corresponds the numbering of the serial port at the external hub Chapter 7 1 E g port 1 at the hub corresponds to the first port installed for the external hub in the Windows system 3 4 Installation of the TProfessional Manager Software Open the file SetupTProfManager200 exe An Install Shield setup routine opens that guides through the installation process Choose a setup language Accept the license agreement Enter your name and the name of your company or institute Choose between complete and user defined installation Optional Change the directory the program is installed to For user defined installation select the files that will be installed You can choose etween the following components a TProfessional Manager Software for controlling TProfessional Thermocyclers b Temperature Profile for the graphical display of temperature data from Detailed Log Files See chapter 6 3 1 and 9 c BiometraShared folder with pre installed PCR programs e Directories e Programs If complete installation has been chosen all
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21. clers are activated To confirm you selection press OK in the Cycler Manager window Note If a program is saved from PC to Thermocyclers programs occupying the same store will be overwritten Therefore always make sure that no important programs are overwritten that you would like to keep Note If a program is saved on different thermocyclers if necessary it will be adapted to the corresponding block type or cycler type 6 6 Open Directories The TProfessional Manager Software allows to open directories saved on the PC or saved on the TProfessional Thermocyclers 6 6 1 Open PC Directory Click on Program in the menu of the TProfessional Manager user interface To open your personal directory on the PC click on Open PC Directory 24 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company Select directory Service Open Cycler directory The Open window appears Look in B PROGRAMS hy Recent Documents E Desktop Mo Documents 5 hy Computer 2 My Metwork File name Places Files of type Select the program you would like to open in the directory PROGRAMS and click on Open 6 6 2 Open TProfessional Thermocycler Directory To access a directory on a TProfessional Thermocycler the instrument has to be specifically addressed in the Cycler View window The field for the actively addressed Thermocycler appears with a white background
22. directory in which the programs are going to be copied Open the PC directory where the programs are installed The standard installation path is C Programs Biometra T ProfessionalManager BiometraShared Programs At the PC choose the appropriate folder corresponding to the Thermocycler see also chapter 8 1 Select a single program from the folder and open it Click on Save Save as select a store number and click on Save on Cycler Repeat this procedure for each single program User Manual TProfessional Manager Software January 2009 41 Biometra An Analytik Jena Company 9 Analysis of Detailed Log Files For the analysis of Detailed Log Files by Microsoft Excel the file Temperature profile V113 xIs has to be installed see chapter 3 4 The Excel file allows the readout of temperature data and the graphical display of Detailed Log Files Open the Excel file Temperature profile V113 xls Note During the installation the file is copied into the folder C Programs Biometra TProfessionalManager unless another installation folder has been chosen during the setup see chapter 3 4 ABCDEFGH 1 JKL MN OP GRIS TT Lv De Y Z AA LALALALA LALALALA ALA LALALA LALALA LALA ALATA AAA BIBIBIBIBIB Detailed Logfile biometra Run Logtile Part of GE Healthcare Clear rinting Time and Date Open fun Logtile of Program Logfile Log Time and Date Start End Time Program User Cyclertype Company Serial No Software
23. dressed by the software can be defined Only COM ports that are installed at computer can be selected For serial ports that are not available the corresponding check boxes appear in grey and cannot be selected COM Port fexisting Ports are enabled Select COM Ports displayed in Cycler View window max 5 W COM 1 P come P coma come coms EE come P com7 PW come M come M comio EH comir M comiz EE comis P comis P comis P comie Select All f Reset Selection Note Depending upon the configuration of the network see chapter 3 3 during the hardware installation process serial COM ports are registered in a numbered order by the PC The TProfessional Manager Software supports up to 5 the serial COM ports Select the appropriate serial ports by activating single check boxes or by activating the button lt Select All gt and confirm with OK For each selected serial port a status view bar is displayed in the window Cycler View up to max 5 ports User Manual TProfessional Manager Software January 2009 11 Biometra An Analytik Jena Company Status COM 2 Cycler Block Status User COM 4 Cycler Block Status User Remaining Time The configuration of the serial ports is only necessary for the first start of the network or when the configuration of the network is changed The TProfessional Manager Software saves the configuration and loads it when the program is started again Note If
24. e create 33 User Manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company send 34 Installation Software 7 Login 17 Network installation 6 Operating Interface 8 PIN Code 31 32 Program create 16 open 15 save 22 start 19 stop 21 Service Info File 29 Setup menu 11 Setup Menu 8 Supervisor 31 Troubleshooting guide 36 Error code 782 38 45
25. em 1 3 Copy directory from PC to Cycler 26 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 1 System Configuration User select topic 11 Set language 1 Change PIN 13 Copy directory from Cycler to PC 14 Copy directory from PC to Cycler 15 Clear directory All programs in user directory will be deleted Cee eee PF Service directory The selected directory is copied by the TProfessional Manager into the preset non changeable folder Directory to the PC After successful file transfer the following message is displayed TProfessional Manager i x A The process was successfull DD All data saved in C Dokumente und Einstellungen holden Lokale Einstellungen 4anwendungsdaten BIOMETR A TProfManager DIRECTORY directory 1902200 AB 20090126 13h25m dix User Manual TProfessional Manager Software January 2009 27 Biometra An Analytik Jena Company 6 7 2 Copy Directories from PC to Cycler Click on the menu button System in the menu bar of the TProfessional Manager user interface From the systems menu select item 1 User Configuration and activate subitem 1 4 Copy directory from Cycler to PC Click on Selection 1 System Configuration User select topic Set language Change PIN Copy directory from Cycler to PC Copy directory from PC to Cycler Clear directory All programs in user directory will be deleted Click on Select File to cop
26. er your supervision PIN code OK lt appears a notification that in the Cycler View window it can be defined to which connected TProfessional Thermocyclers the user can log in 18 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company Enter 7 digit PIN code for AA Pc User To use multiple cycler Control check boxes in Cycler View window By activating deactivating the check box Login in the status view of the Cycler View window you can select to which Thermocyclers you will log in By routine on the start of the program the check boxes of all available TProfessional Thermocyclers are activated To confirm your selection press OK in the Cycler Manager window Note If a false pin code is entered the message Access denied incorrect Pin is shown in the Cycler Manager window and Incorrect PIN in the Thermocycler status view in the Cycler View window COM 3 Login Se Save Detailed Logfile Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Incorrect PIN User Remaining Time 6 3 Start Program When a program has been selected for start click on the button Start in the Cycler Manager window The following message appears in the user interface User Manual TProfessional Manager Software January 2009 19 Biometra An Analytik Jena Company Select program name or enter program number Start Start program o
27. fessional Thermocycler to that an Image File shall be send The field for the actively addressed Thermocycler appears with a white background COM 3 fe FM Save Detailed Logfile Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Block free User 44 Remaining Time Note You can only send Image Files to Thermocyclers that are addressed by the Manager Software 34 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 4 Send image to cycler Press softkey Select File to send to select an image file Existing user and programs will be overwritten with selected irmage tile After restart cycler programs willbe adapted to cyclertype if necessary Image Expvers_200007 30_14h33m imag a Hj Image_Expers_20080731_13h45m img Zuletzt Image Expvers_20050731_1TbhU5m img verwendete Desktop Eigene Dateien Aee Dateiname ung Dateityp Abbrechen Select the Image File to be send and confirm your selection by clicking on Open The selected Image File will be send to the TProfessional Thermocycler Note During the transfer of an image file first the complete memory is deleted and then the data of the image file is transferred Note If necessary programs will be adapted the specific type of Cycler For example heating and cooling rates may be adapted if programs are transmitted from a TProfessional or TProfessional Standard Thermocycler t
28. ge occurs if the serial communication to one or several Thermocyclers cannot be established This may happen if the Connect button in the Cycler View window has been clicked see chapter 5 2 while at least one Thermocycler has not yet passed the initial self test completely Since the Thermocycler is still busy the connection initiation fails The TProfessional Manager Software then is waiting for a serial signal from the Thermocycler and is in loop process The program will not react to user input for 2 minutes Please do not close the software and do not switch off the Thermocyclers Wait for 2 minutes and start the connection initiation by clicking the Connect button again see chapter 5 2 7 3 Message Cycler on COM Port not available If a serial port is selected that is installed at the computer and entered in the Windows registry but no TProfessional Thermocycler is connected to or if the Thermocycler is switched off or not ready to the following message is shown TProfessional Manager Fa X Cycler on Com Port number 13 not available Check connector Warming IF block is active record data can be missing Make a note for all of COM ports for that the error message has occurred Ensure that all TProfessional Thermocyclers are switched on and have passed the initial check up Inactivate all serial ports in the Setup menu see chapter 5 1 for that you have received the error message and reconnect the remaining
29. gfile Cycler TProfessional Block Gradient 96 Status starting program Remote control User HD eee Remaining Time 1h 40m Start the program Chapter 6 3 Note To analyse Detailed Logfiles you can use the file Temperature_profile xls see chapter 9 if the file has been copied to the computer during the installation of the TProfessional Professional Manager Software see chapter 3 4 20 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 6 4 Stop Program Press the button Stop in the Cycler Manager window The following message is displayed in the user interface Program hata 88 01 biometra Stop active program It appears a notification that in the Cycler View window it can be defined at which connected TProfessional Thermocyclers the program will be stopped To use multiple cycler Control check boxes in Cycler View window By activating deactivating the check box Stop in the status view of the Cycler View window you can select at which Thermocyclers the program will be stopped User Manual TProfessional Manager Software January 2009 21 Biometra An Analytik Jena Company Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Block active Remote control User AA To confirm you selection press OK in the Cycler Manager window 6 5 Save Program Created or edited programs can be saved on the PC or
30. id ON ie Pos ms goto loops so owo 7 95 0 rol _ 620 Joo 72 0 fous 2 a oetions 720 o 60 Pase _ The window Save as is opened Enter a name for the program in the field file name Press the button Save to save the program Save irr PROGRAMS x My Recent Documents Desktop My Documents hy Computer My Network File name hes Places Save as type Thermocycler Program Files tcp Cancel Note The TProfessional Manager saves the new program with the file extension Thermocycler Program File in the directory PROGRAMS of the Windows user account tcp 6 2 Log in Menu After connecting TProfessional Thermocyclers Chapter 5 2 to the PC the start screen changes and the directory of the actually addressed thermocycler is displayed in the user interface of the TProfessional Manager Moreover the menu buttons Login out Setup and Block become activated and are clickable Select a user account from the list and click on Log in to log in To log in as supervisor click on Supervisor Log in User Manual TProfessional Manager Software January 2009 17 Biometra An Analytik Jena Company To log in select user or enter first letter PP Service Log in In the following window enter the PIN code and confirm with PIN code OK Enter 7 digit PIN code for UG Uolker Remember to log out when this cycler is no longer und
31. n cycler Save Detailed Logfile Create Detailed Logfile for this run To select the Thermocylers at which the program will be started activate deactivate the check box Start for each Thermocycler in the Cycler View window COM 5 I Stark Cycler TProfessional Block Gradient 96 Status Block Free User HD Remaining Time To confirm your selection press OK in the Cycler Manager window Note If a program is started on instruments via the TProfessional Manager Software and if it does not exist on the thermocyclers it is saved at the designated store to the actually opened user directory Existing programs will be overwritten Therefore always make sure that no important programs are overwritten that you would like to keep The most suitable practice is to save the program to a free store on the thermocycler before starting it Note If a program is started on serveral different Thermocyclers the program is if necessary adapted to the corresponding type of block cycler 6 3 1 Documentation of Detailed Log Files The TProfessional Manager offers a feature for the documentation of detailed temperature data during a run When using this feature each second a temperature profile for the thermoblock is recorded and saved by the software Activate the check box Save Detailed Logfile in the Cycler View window for each single Thermocycler a detailed log file shall be recorded COM 5 7 Start gt VV save Detailed Lo
32. name in the field File name for the file to be saved and click on Save Save in DETAILED_LOGFILES My Recent Documents Desktop Mu Network File name il Logfile 2809232 Places Save ag type Text Files tet Cancel Send the file to the Biometra Service Department service biometra de The file is saved in the path lt User Files T ProfManager V100 Detailed_Logfiles gt on the computer 30 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 6 9 Language settings To set the system language select System in the TProfessional Manager operating interface In the system menu activate User configuration and menu item 1 1 Select language an You can choose between German and Englisch Note Only the language of the TProfessional Manager programming interface is changed see also chapter 4 The language for the menus and operating interface are not affected The language setting for the Thermocyclers has to be done at the instruments 6 10 Supervisor System Menu The supervisor has access to an own system menu with some specific functions System configuration Supervisor topic list 1 Change user PIN 2 Change SUP PIN 3 Create image on PC 4 Send image to cycler 6 10 1 Change user PIN The supervisor can change the PIN for each user account Select a user directory from the list and press Select User User Manual TProfessional Manager Software Jan
33. nciple the user interface of the TProfessional Manager is designed like the screen of the TProfessional Thermocyclers Please read the corresponding chapters in the TProfessional Thermocycler manuals if you have basic question for the handling Subsequently it is described to what extent the user interface of the TProfessional Manager differs from the display on the instruments and which additional functions are integrated in the software 6 1 Start screen After the TProfessional Manager is started in the Cycler View window the following start screen is shown Logout Biok Program Connect to cycler or edit existing programs Work on PC You can write or edit programs an PC Please press softkey New program or Open PC program To start PC program save on cyler and press Start Work on cycler You can write or edit programs start them and record logfiles on PC To work on cycler press button ConnectDisconnect in cycler view window Program PC directory As long as there is no TProfessional Thermocycler connected to the PC the menu buttons Login Out System and Block are inactive and are displayed in grey The menu button Program is active and programs stored on the PC can be opened Chapter 6 1 1 or new programs can be created and stored on the PC Chapter 6 1 2 6 1 1 Open a program stored on the PC TO open a program stored on the PC click on Open PC directory in the start screen of the TProfe
34. o a TProfessional Basic Thermocycler Accordingly gradient protocols become adapted if these are transmitted from a gradient enabled TProfessional Thermocycler to a non gradient Thermocycler The temperatures at this step will normally be set to the middle temperature of the gradient Please check the transmitted protocols and if applicable adapt them to your specific needs User Manual TProfessional Manager Software January 2009 35 Biometra An Analytik Jena Company 7 Troubleshooting guide 7 1 Check configuration of serial ports at the computer Most computers have one serial port that is installed as COM port 1 Depending upon the individual configuration of the computer some additional true or virtual COM ports may be added that are registered after COM port 1 by the system During the installation of an internal PCI serial card or an external serial hub see chapter 3 3 the newly installed COM ports become automatically numbered by the computer Depending upon the number of already occupied serial ports the newly added serial ports become ordered by the following next free numbers Note If serial serial adaptors become installed via USB for each USB port the added serial ports will be recognozied as new by the Windows Plug amp Play function and will be installed with new numbers If the USB port was repeatedly changed the serial ports will be installed with numbers higher than 16 by the system and can no longer addressed by the
35. pper part of the operating interface and the context sensitive menu buttons and the Back button in the lower part can be activated by mouse clicks Moreover the buttons Start and Stop to start and stop programs are clickable To navigate in the inner user interface use the cursor keys and the return key of the PC keyboard To enter numbers and characters also use the PC keyboard In the lower part of the Cycler Manager window a status view display for the addressed Thermocycler is integrated User manual TProfessional Manager Software January 2009 9 a Display if no Thermocycler is connected Status not connected Cycler Block User Biometra An Analytik Jena Company Display when Thermocycler is connected Status connected COM 1 Cycler Professional Block Gradient 96 User AA Status serial port the Thermocycler is connected to Cycler instrument type Block block type used on the Thermocycler User logged in user As long as no Thermocycler is connected to the PC the message not connected is displayed in the status view display A AS soon as a connection has been established the actually addressed type of Thermocycler is displayed B 4 3 Cycler View Window The Cycler View window displays a status message for each Thermocycler connected to a specific serial port C Display if no Thermocycler is connected COM 1 Cycler Block Status I Save Detailed Logfile User
36. ssional Manager Chapter 6 1 The window open appears User Manual TProfessional Manager Software January 2009 15 Biometra An Analytik Jena Company Look in B PROGRAMS A hy Recent Documents Desktop My Documents PE hay Tonde z My Network File name ae bal Places Files of type Thermacycler Pragram Files tcp Cancel Select the program you would like to edit from the directory PROGRAMS and click on Open In the user interface of the TProfessional Manager the opened program is displayed LoginOut System Block Program Edit program 00 Block type 96 well Preheat Lid ON BME 06 Steps aE mis goto loops 1 so joo f 2 a et f ax 3 550 00 30 options L 4 s 720 fosoj 6 16 0 Pause l ll Note The TProfessional Manager creates a folder PROGRAMS in the personal directory for each single user that is logged in to Windows Hence in the folder PROGRAMS only the stored programs of the currently logged in user are shown 6 1 2 Create a new program Click on New Program in the start screen of the TProfessional Manager Chapter 6 1 to create a new program Enter the new program To save the program select Save Save As in the user interface of the TProfessional Manager 16 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company LoginQut j j Program Edit program Block type 96 well Preheat L
37. the following message should appear Cycler on COM Port number xx not available please read chapter 7 3 5 2 Establish a connection to TProfessional Thermocyclers Press the button lt Connect Disconnect gt in the Cycler View window of the TProfessional Manager if you would like to establish a connection of the Manager Software to the Thermocyclers The Connect Disconnect window opens Connect Disconnect Cycler Disconnect Select All Select All Connect Disconnect Connect l Disconnect Activitate the check box lt Connect gt for each serial port you would like to connect or select all serial ports to be connected by activating the check box lt Select All gt Press the button lt Connect gt The Connect Disconnect window is closed by the TProfessional Manager and subsequently the connected TProfessional Thermocyclers are displayed in the Cycler View window 12 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company COM 4 fa Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Glock free User Remaining Time COM 5 Cycler TProfessional Block Gradient 60 Status Block free User Remaining Time The field for the actively addressed Thermocycler appears with a white background Furthermore a black arrow is displayed next to the field directing to the neighbouring window In the Cycler Manager window the status view for the acti
38. uary 2009 31 Biometra An Analytik Jena Company SUP Change user PIN select user PF Service Bock Program Enter new PIN Repeat PIN By activating deactivating the check box Change PIN in the status view of the Cycler View window you can select at which Thermocyclers the user PIN will be changed COM 1 Change PIN Cycler TProfessional Block Gradient 60 Status Block free User SUP Remaining Time 6 10 2 Change supervisor PIN Enter your actual PIN the default Supervisor PIN is 000 000 0 do not enter blanks enter your new PIN and repeat your input 32 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company SUP Change SUP PIN SUP WAttention Forgotten SUP PIN can only be changed by Biometra Service in Germany Enter current PIN Enter new PIN Repeat PIN Confirm with PIN OK in the Cycler Manager window By activating deactivating the check box Change PIN in the status view of the Cycler View window you can select at which Thermocyclers the superuser PIN will be changed COM 1 gt Change PIN Cycler TProfessional Block Gradient 60 Status Block free User SUP Remaining Time Note Please make a note of the Supervisor PIN und keep it on a safe spot A forgotten Supervisor PIN can only be reset by the Biometra Service Department and requires to send back the instrument 6 10 3 Create Image File This f
39. unction allows to copy all folders of a TProfessional Thermocycler The content of all user directories and programs therein will be stored in a file on the PC Select the TProfessional Thermocycler for that an Image File shall be created in the window Cycler View The field for the actively addressed Thermocycler appears with a white background COM 3 M I Save Detailed Logfile I Cycler TProfessional Basic Gradient Block Grad 96 Basic Status Block free User 44 Remaining Time Note You can only create Image Files for Thermocyclers that are connected to the computer and addressed by the Manager Software if you are logged in as supervisor see chapter 6 2 User Manual TProfessional Manager Software January 2009 33 Biometra An Analytik Jena Company 3 Create image on PC Press softkey Create image to create an memory image file an PC All userdirectories and programs will be copied Filename is Image Serialno cycler DateTirme img To create an Image File confirm by clicking on Create image The file will automatically be stored in a special folder on the PC All Image Files are stored with the date and time when they have been created 6 10 4 Send Image to Cycler By sending an Image file to a TProfessional Thermocycler stored user directories and programs become transmitted Prerequisite is the existence of an Image file created afore Chapter 0 In the window Cycler View select the TPro
40. vely addressed Thermocycler is displayed Status connected COM 4 Cycler TProfessional Basic Gradient Block Grad 96 Basic User Note If problems during the setup of serial ports should occur in chapter 7 1 a comprehensive description how to check to configuration of serial ports at the computer is given Note If you receive a message with error code 782 Cycler didn t answer repeat procedure 782 please read chapter 7 2 5 3 Disconnect TProfessional Thermocyclers Press the button lt Connect Disconnect gt in the Cycler View window of the TProfessional Manager if you would like to disconnect TProfessional Thermocyclers The window connect Disconnect opens Addressed Thermocyclers are detected automatically by the TProfessional Manager software Connect Disconnect Cycler Disconnect Select Al W Select All Connect if Disconnect Activate the check box lt Disconnect gt for each serial port you would like to disconnect or select all serial ports by activating the check box lt Select All gt Press the button lt Disconnect gt The User Manual TProfessional Manager Software January 2009 13 Biometra An Analytik Jena Company connect Disconnect window is closed by the TProfessional Manager and the chosen Thermocyclers become disconnected 14 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company 6 Operating the TProfessional Manager In pri
41. y in the next window 1 4 Copy directory from PC to cycler Press softkey Select File to copy to select a file generated with 1 3 Copy directory from cycler to PC Directory 44 will first be deleted and then overwritten with selected directory file Write permission only in personal directory to copy A new window opens and displays the available directories of the user that is logged in to the PC Select the directory to copy and confirm by clicking on the button Open The TProfessional Manager copies the contents of the selected directory into the user directory of the logged in user During this process existing programs at first are deleted and then replaced by the programs of the selected PC directory 28 User manual TProfessional Manager Software January 2009 Biometra An Analytik Jena Company Look in B PROGRAMS A hy Recent Documents Desktop My Documents hy Coneis z My Metwork File name tepl i Places Files of type Thermacycler Pragram Files tcp Z Cancel Note As for the Thermocyclers the logged in user can only edit and delete his own directory and programs The user can open and copy but not edit or delete other directories and programs When directories and programs are copied only the directory of the user who is logged in at a Thermocycler become modified other directories will not be overwritten Note If a directory is saved on several different Thermocyclers

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