EASY-Spray Series Ion Source User Guide
Contents
1. 3 mm gap between the ion transfer tube EASY Spray column and an actual emitter J shown without its cable lon transfer tube Thermo Scientific EASY Spray lon Source User Guide 11 2 Installing the EASY Spray Source Adjusting the Emitter Tip Position To adjust the emitter tip position Tip To enhance your view of the emitter positioning tool s tip install the video camera and monitor before you begin this procedure For instructions see Chapter 3 Connecting the LCD Monitor and Video Camera 1 Retract the z axis front to back position by turning the control knob located on the column holder counterclockwise Figure 11 2 Insert the emitter positioning tool into the top of the column holder and then slowly slide it forward until it clicks into place 3 Align the x axis side to side position of the emitter positioning tool to the bore of the ion transfer tube by inserting the hex key into the left side hex socket head screw Figure 11 Figure 11 X and z axes position controls on the EASY Spray source ag pe ee Inserting the emitter positioning tool i CH tC 6 Z axis position control knob X axis position control hex socket head screw 12 EASY Spray lon Source User Guide Thermo Scientific Thermo Scientific 2 Installing the EASY Spray Source Adjusting the Emitter Tip Position 42. 3 Power adapter cable The total length of the output cord and power adapter cable is approximately 9 ft 274 cm Make sure that there is an electrical wall outlet within reach for the power supply unit and that you provide suitable clearance around it for cooling EASY Spray lon Source User Guide 5 LLL m Installing the EASY Spray Source Follow these procedures to install or remove the EASY Spray source The only tool required is the 3 mm hex key Note The complete EASY Spray source installation including the digital camera and LCD monitor requires three electrical wall outlets Ensure that your lab has the appropriate outlets near the nanoLC MS system Contents Installing the Ion Source onto the Mass Spectrometer Connecting the Controller Power Supply e Adjusting the Emitter Tip Position Removing the EASY Spray Source Thermo Scientific EASY Spray lon Source User Guide 7 2 Installing the EASY Spray Source Installing the lon Source onto the Mass Spectrometer Installing the lon Source onto the Mass Spectrometer Before you install the EASY Spray source follow the procedure to prepare the mass spectrometer lt To prepare the mass spectrometer 1 If installed remove any other source from the mass spectrometer after it has cooled to room temperature For instructions refer to the mass spectrometer s documentation CAUTION HOT SURFACE Accidental contact with the part of the API probe locat
3. 2012 Thermo Fisher Scientific Inc All rights reserved Acclaim EASY Column EASY nLC EASY Spray Exactive Foundation nanoViper PepMap Q Exactive Velos Pro and Viper are trademarks and Dionex LCQ LTQ Orbitrap Thermo Scientific UltiMate and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc in the United States The following are registered trademarks in the United States and other countries Dino Lite is a registered trademark of AnMo Electronics Corporation IDEX is a registered trademark of IDEX Health amp Science LLC Microsoft and Windows are registered trademarks of Microsoft Corporation PEEK is a trademark of Victrex plc All other trademarks are the property of Thermo Fisher Scientific Inc and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and
4. 23827 0008 medium 23827 0009 large Two column setup Any nanoViper equipped precolumn This image shows the Acclaim PepMap100 Nano Trap For the PepMap100 Nano Trap Fisher Scientific 164705 Viper union for use with the EASY nLC 1000 Thermo Scientific Dionex 6040 2304 EASY Spray lon Source User Guide ZDV adapter union 1 16 to 1 32 in and plug not shown for use with the EASY nLC II UltiMate 3000 RSLCnano EASY Spray Application Kit IDEX P 881 Thermo Scientific Dionex 6720 0395 Thermo Scientific 4 Connecting the LC to the EASY Spray Column Installing the EASY Spray Column for the First Time Installing the EASY Spray Column for the First Time The EASY Spray column Figure 14 contains an integrated heatable column and a fused silica emitter For available columns see Chapter 8 Replaceable Parts IMPORTANT The EASY Spray column assembly includes an emitter protective cover If you push back this cover by hand you could damage the fused silica emitter and make the EASY Spray column unusable Figure 14 EASY Spray column nanoViper fitting for the LC connection mE Emitter protective cover Protective cap Heater cable and plug l To install the EASY Spray column 1 Follow the procedure To adjust the emitter tip position on page 12 Note You can skip this step after the first time if you have not adjusted the column ho
5. 20 um ID valves WPS 3000RS EASY Spray lon Source User Guide Thermo Scientific MEN E Configuring the Mass Spectrometer for NSI Mode Follow the applicable procedures to configure the Thermo Scientific mass spectrometer for nanoelectrospray ionization nanoESI or NSI mode Contents Selecting Nanospray as the Ion Source Configuring the nanoESI NSI Source Parameters Selecting Nanospray as the lon Source Note This section is for the LCQ Deca XP Max mass spectrometer which uses the Thermo Xcalibur data system version 2 0 7 or earlier After you install the EASY Spray source use the Instrument Configuration window to configure the mass spectrometer for NSI mode To configure the mass spectrometer for NSI mode 1 If open close the Xcalibur data system and Tune Plus application 2 On the Microsoft Windows taskbar choose Start gt All Programs gt Xcalibur gt Instrument Configuration to open the Instrument Configuration window 3 Select the devices to control from the Xcalibur data system if not already selected a In the Device Types list select All b Under Available Devices double click the icons for the mass spectrometer and nanoelectrospray LC instrument to add them to the Configured Devices list 4 Double click the mass spectrometer icon to open the Model Configuration dialog box 5 In the left pane select Ion Source to display the ion source configuration page and then
6. Align the y axis up and down position of the emitter positioning tool to the bore of the ion transfer tube by inserting the hex key into the bottom hex socket head screw Figure 12 Figure 12 Y axis position control on the EASY Spray source J m KL Y axis position control AN KO 5 Adjust the z axis front to back position of the emitter positioning tool s tip by turning the control knob located on the front of the column holder Figure 11 on page 12 Move the tool s tip so that it gently touches the bore of the ion transfer tube IMPORTANT The fused silica emitter is fragile Make sure that the emitter positioning tool gently touches the ion transfer tube when you adjust its position or you will damage the actual emitter when fully inserted requiring you to replace the EASY Spray column 6 After you adjust the tool tip s position slowly remove the emitter positioning tool The position of the column holder is now calibrated for use with any EASY Spray column You must repeat this procedure if you later change the x y or z axis position EASY Spray lon Source User Guide 13 2 Installing the EASY Spray Source Removing the EASY Spray Source Removing the EASY Spray Source Follow this procedure if you need to remove the EASY Spray source from the mass spectrometer S Ze To remove the source from the mass spectrometer In the Tune Plus application place the m
7. If you need further troubleshooting assistance contact your local Thermo Fisher Scientific field service engineer See Contacting Us on page xv Table 7 EASY Spray source problems causes and solutions Sheet 1 of 2 Problem Cause Solution The spray is There is a leak somewhere in Check all of the LC connections unstable the liquid path Air bubbles in the emitter might Try degassing the mobile phase or purging the line and cause the spray to spit then recheck the line for air bubbles The EASY Spray column is out Follow the procedure Adjusting the Emitter Tip Position of alignment on page 11 The source high voltage HV Check the high voltage contact on the back of the connection might be unstable EASY Spray source Figure 7 on page 9 and the front of the mass spectrometer If the problem continues do the following 1 Open Tune Plus choose View Display Status View and then click the Alll tab 2 Choose Setup NSI Source to open the NSI Source dialog box 3 Compare the setting in the NSI Source dialog box to the readback value for the NSI source absolute spray voltage A green check mark v Spray Voltage kv indicates that the parameter is functioning properly There is an emitter blockage Try adjusting the spray voltage If that does not resolve the from particles in the sample blockage problem insert a new EASY Spray column other small particles from the flow lines or valves and s
8. N ES081 Sheet 1 of 2 Image Description Quantity Part number al EASY Spray source housing 1 EASY Spray column 15 cm x 3 um ID 1 ES800 xii EASY Spray lon Source User Guide Thermo Scientific Preface Table 2 EASY Spray lon Source Kit P N ES081 Sheet 2 of 2 Image Description Quantity Part number Controller power supply assembly 1 ES231 E De m Power supply unit 24 Vdc output shown without the input plug Power adapter cable not shown Dino Lite digital video camera and 1 ES216 power supply unit not shown Monitor power supply unit and BNC 1 ES217 adapter not shown Tools l set ES232 e Emitter positioning tool 3mm hex key 1 5 mm hex key 1 A B mixing venting tee stainless steel l set SC901 with three attached ferrules and nuts Sleeves PEEK 1 16 in OD 3 IDEX F 233 To order another EASY Spray source housing you must order the EASY Spray Ion Source Kit P N ES081 Thermo Scientific EASY Spray lon Source User Guide xiii Preface Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by an appropriate CAUTION symbol IMPORTANT Highlights information necessary to preve
9. assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history Revision A May 2012 For Research Use Only Not for use in diagnostic procedures e AA Contents it a eee Tere rr ra oa ee ret xi Product Requirements va sa Re ER ER ERR ERO Eee OR ER che ORA xii Contents of the EASY Spray Ion Source it 24 454 easet xii Safety and Special Notices AEN EEN Peer a sens bags xiv Contacting Us cedes tii pas tren ex a rre bx a ae xv Chapter Introduction Mr 1 Advantages of Nanoelectrospray d 2 9 xe d ln CERE OUR Re RR alii 2 Functional Description near an 2 Source Housing cosa to kr EN ea ex e ERR a 3 Conor e cassa eese Vcr wanted ER bandi dpa 4 Controller Power Supply 552a a geck be oen en 5 Chapter2 Installing the EASY Spray Source 7 Installing the Ion Source onto the Mass Spectrometer 8 Connecting the Controller Power Supply SEN ces cae a0 eee eens 10 Adjusting the Emitter Tip Position see nen cies Re eek vane 11 Removing the EASY
10. controller PCB includes an input power socket an output heater socket a temperature control dial and an actual temperature display Figure 3 Controller PCB located on the bottom of the EASY Spray source Actual temperature display Controller PCB not visible SP amp amp 1 Input power connection 24v BEZow TW The controller power supply unit connects to the input power socket which is located on the bottom right side of the EASY Spray source The cable from the EASY Spray column connects to the heater socket which is located on the front and labeled Heater The temperature control dial has an OFF setting and temperature settings in 5 C increments for the 30 60 C range As the column heats up the actual temperature appears on the display Thermo Scientific 1 Introduction Functional Description Controller Power Supply Thermo Scientific Figure 4 shows the controller power supply assembly that consists of a wall mounted power supply unit with a detachable input plug and a power adapter cable The other end of the power adapter cable connects to the input power socket located on the bottom of the EASY Spray source Figure 3 on page 4 and Figure 8 on page 10 to provide the 24 Vdc input for the controller PCB Figure 4 Controller power supply assembly shown with a European input plug m Power supply unit with output cord
11. ferrule and qut La cicer dE AE 26 Figure 20 Column Out line with smaller fitting from the ZDV adapter union 27 Figure 21 Example of a two column setup for the EASY nLC II 28 Figure 22 Example of a one column setup for the RSLCnano system direct injection sanas omiani e eC CR REOR E Eabb eR dl Pap de o eier 29 Figure 23 Example of a two column setup for the RSLCnano system e EE 30 Figure 24 Tune Plus window for an NSI configured Velos Pro example 32 Figure 25 NSI Source dialog bots wii vedere E dob da ee rane eee ER 33 Figure 26 Controller PCB removed from the EASY Spray source 36 Figure 27 Bottom of the EASY Spray source without the controller PCB 37 Thermo Scientific EASY Spray lon Source User Guide V Regulatory Compliance Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations When the system is delivered to you it meets all pertinent electromagnetic compatibility EMC and safety standards as described in the next section or sections by product name Changes that you make to your system may void compliance with one or more of these EMC and safety standards Changes to your system include replacing a part or adding components options or peripherals not specifically authorized and qualified by Thermo Fisher Scientific To ensure continued compliance with EMC and sa
12. for 5 replacing 36 E EASY Spray column assembly 21 installing 21 EASY Spray ion source connecting controller power supply unit 10 monitor and camera 15 EASY Spray column installing 21 housing description 3 installing 9 kit contents xii replaceable parts 41 locking levers positions 9 power connection 10 removing 14 x and z axes controls 12 y axis control 13 electrical wall outlets 7 electromagnetic compatibility vii EMC compliance vii Thermo Scientific emitter adjusting the tip position 12 troubleshooting 39 environmental requirements xii F FCC compliance vii figures list v G gloves part numbers 20 H heater troubleshooting 40 high voltage connector location 9 internal cable location 37 troubleshooting 39 housing see EASY Spray ion source jon source see EASY Spray ion source ion sweep cone 8 K kits EASY Spray ion source xii UltiMate 3000 RSLCnano EASY Spray Application 28 EASY Spray lon Source User Guide 45 Index L L LC connections EASY nLC 1000 connecting to 23 EASY nLC II connecting to 26 RSLCnano system connecting to 28 tools and supplies 19 locking levers positions 9 maintenance controller PCB replacing 36 guidelines 35 tools and supplies 35 mass spectrometer diagnostics 39 ion source removing 14 ion sweep cone 8 NSI mode manually LCQ Deca XP Max 31 power requirements xii preparing for the ion source 8 setting the NSI source parameters 32 monitor conne
13. heater cable routed above the controller PCB Figure 26 Controller PCB removed from the EASY Spray source De f Actual Temperature Set E mperature P Le is y e e re i ern sof Mig Hester Scien i Wl SEN aA Wl meo eA UA Exsy Spray 4 Unpack the new controller PCB and make sure it is not damaged CAUTION To prevent damage to the electronic components on the PCB due to ESD hold the new controller unit by the metal enclosure instead of the PCB 36 EASY Spray lon Source User Guide Thermo Scientific Thermo Scientific 6 Maintenance Replacing the Controller PCB 5 Slowly push the new controller PCB enclosure into place and route the heater cable so that it does not become damaged pinched Figure 27 Figure 27 Bottom of the EASY Spray source without the controller PCB r High voltage cable connects to the column holder Hole for the hex socket head screw 6 Using the 2 5 mm hex key install the four hex socket head screws into the bottom of the controller PCB enclosure You can now install the EASY Spray source back onto the mass spectrometer To dispose of the old controller PCB see WEEE Compliance on page ix EASY Spray lon Source User Guide 37 MEN O Troubleshooting Table 7 lists some EASY Spray source and column problems their causes and their possible solutions You can resolve most problems by using a new EASY Spray column
14. nanoLC MS system using the EASY Spray ion source camera and DODItOI nee ke pre en weap avers acides or eb ed daddy a 1 Figure 2 EASY Spray source housing id sed Ek Bee 3 Figure 3 Controller PCB located on the bottom of the EASY Spray source 4 Figure 4 Controller power supply assembly shown with a European input plug 5 Figure 5 Jon sweep cone removed from the mass spectrometer s spray cone 8 Figure 6 Locking lever pasttlaliss Len eat aie sistema 9 Figure 7 Ion source housing connection iss cios KEEN EE EEN tante CE ERR 9 Figure 8 Power connection for the controller PCB ann 10 Figure 9 Emitter positioning tool esa beso Seba dE AR bb ER parere 11 Figure 10 Gap positions in front of the mass spectrometer s ion transfer tube 11 Figure 11 X and z axes position controls on the EASY Spray source 12 Figure 12 Y axis position control on the EASY Spray source 13 Figure 13 Camera and column installed on the EASY Spray source 16 Figure 14 EASY Spray column eeh cee cede coarse erase oe chew d ed erba 21 Figure 15 mo Viper fitting ENEE AEN ENEE A ds 22 Figure 16 Recommended plumbing locations on the venting tee nanoViper example 24 Figure 17 Example of a one column setup with nanoViper fittings 24 Figure 18 Example of a two column setup with nanoViper fittings and a Viper pun qmm 25 Figure 19 EG line with sleeve
15. select Nanospray in the Default Source list 6 Click OK and then click OK again to close the message box 7 Configure the LC device if you have not done so For instructions refer to the LC instrument documentation Thermo Scientific EASY Spray lon Source User Guide 31 5 Configuring the Mass Spectrometer for NSI Mode Configuring the nanoESI NSI Source Parameters 8 In the Instrument Configuration window click Done 9 Restart the data system computer and the mass spectrometer Configuring the nanoESI NSI Source Parameters After you complete the instrument configuration use the Tune Plus application to configure the NSI source parameters in the NSI Source dialog box Li To set the NSI source parameters 1 Do one of the following to open the Tune Plus window Figure 24 On the Windows taskbar choose Start gt All Programs gt Thermo Instruments gt model gt model Tune where model is your specific mass spectrometer ON LINE n the Xcalibur application click the Roadmap View icon the Instrument Setup icon the model MS icon and then Tune Plus ve uda ooo ep jt or In the Xcalibur application click the Roadmap View icon the Instrument Setup icon and the model MS icon Then from the main toolbar choose model gt Start Tune Plus Figure 24 Tune Plus window for an NSI configured Velos Pro example Icon for the NSI source f Thermo Tune Plus Untitled File View Contro
16. 21 Example of a two column setup for the EASY nLC II Column Out line EASY Spray column Waste In line Connecting an RSLCnano System to the EASY Spray Column 28 Follow the appropriate procedure for a one or two column setup The two column setup includes a precolumn connected across the autosampler valve Note To connect the RSLCnano system to the EASY Spray column you must use the Thermo Scientific Dionex UltiMate 3000 RSLCnano EASY Spray Application Kit P N 6720 0395 To plumb a one column setup from an RSLCnano instrument To plumb a two column setup from an RSLCnano instrument on page 30 RL 1 To plumb a one column setup from an RSLCnano instrument Insert the nanoViper fitting on the 20 pm ID nanoViper capillary into port 5 of the autosampler valve Figure 22 Insert the fitting on the other end of the capillary into the Viper union tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn 45 90 degrees Do not tighten the fitting by more than a 1 4 turn CAUTION Because the fittings seal against each other inside the union do not tighten them by more than 1 4 turn 90 degrees Overtightening the fittings can irreparably damage their sealing surfaces For additional information about nanoViper fittings see Using the nanoViper Fitting on page 22 EASY Spray lon Source User Guide Thermo Scientific Thermo Sc
17. FAX siemens 43 0 1 333 50 34 26 Belgium Clintonpark Keppekouter Ninovesteenweg 198 B 9320 ERMEBODEGEM AALST E mail service sid benelux thermofisher com Phone men 32 0 24 82 30 30 als eae Ae tre 32 0 24 82 30 31 Denmark Fruebjergvej 3 2100 Kabenhavn Y E mail service sid dk thermofisher com Phone 45 70 23 62 67 F X5 tedio cine 45 70 23 62 63 xvi EASY Spray lon Source User Guide Finland see Sweden Norway and Finland France Also representing French speaking North Africa Algeria Morocco and Tunisia 16 Avenue du Qu bec Silic 765 Z A de Courtaboeuf F 91963 Les Ulis C dex E mail service sid lesulis thermofisher com Phone 33 0 160 92 49 50 Fauna iia 33 0 160 92 48 99 Germany Im Steingrund 4 6 D 63303 Dreieich E mail service dreieich thermofisher com Phone 49 0 6103 408 1050 FAX sun 49 0 6103 408 1213 Italy Strada Rivoltana I 20090 Rodano Milano E mail assistenza tecnica it thermofisher com Phone Numero Verde 800 823 162 FAX sec uiae meines 39 02 9532 0225 Netherlands Takkebijsters 1 NL 4817 BL Breda E mail service sid benelux thermofisher com Phone mee 31 0 76 57 95 555 g cpm 31 0 76 58 10 961 Norway see Sweden Norway and Finland Spain C Valportillo I n 22 1a Planta Edificio Caoba ES 28108 Alcobendas Madrid E mail service sid
18. Spray Source 2 444 eaa 240 44er choked sale 14 Chapter3 Connecting the LCD Monitor and Video Camera 15 Connecting the Monitor and Camera nen ne 15 Adjusting the Video Pere voe x ve x le 17 Chapter4 Connecting the LC to the EASY Spray Column 19 Tools and EE 19 Installing the EASY Spray Column for the First Time oooooomm 21 Using the nanoViper Fitting Yen Meee epee eed retiens 22 Connecting the LC to the EASY Spray Column 23 Connecting an EASY nLC 1000 Instrument to the EASY Spray Column 23 Connecting an EASY nLC II Instrument to the EASY Spray Column 26 Connecting an RSLCnano System to the EASY Spray Column 28 Chapterb Configuring the Mass Spectrometer for NSI Mode 31 Selecting Nanospray as the Ton Source da e SNE a nener 31 Configuring the nanoESI NSI Source Barameters om 32 Thermo Scientific EASY Spray lon Source User Guide iii Contents iv Chapter 6 Chapter 7 Chapter 8 EASY Spray lon Source User Guide Maintenance Med mE 35 Tools and AAA di qnt evel aio de relate de 25 Replacing the Controller PEDIA VPE RE E 36 Troubleshooting m llckx eher a 39 Replaceable Parts 2 Spare Partsi Ach cun e n EES 4 Consumables oo cede o hae E 4 GRAN er an anna en 43 O UT 45 Thermo Scientific ENS E Figures Figure 1
19. ass spectrometer in Standby mode For instructions refer to the mass spectrometer s documentation Turn the source s temperature dial to the OFF position CAUTION HOT SURFACE Although below the maximum safety limit of 70 C 158 F the EASY Spray source can reach approximately 50 C 122 F with the heater unit turned on which can severely burn you Allow the source to cool to room temperature approximately 20 minutes before you touch it Turn off the LC instrument Disconnect the LC plumbing from the EASY Spray column and then remove the EASY Spray column Note Do not pull on the cable from the EASY Spray column Use the plug to disconnect the column from the heater socket Remove the camera as follows a Unplug the camera power supply unit from the electrical wall outlet b Disconnect the cameras yellow video connector from the back of the monitor and the black power connector from the camera power supply unit c Using the 1 5 mm hex key loosen the hex socket head screw securing the camera and then remove the camera Unplug the controller power supply unit from the electrical wall outlet and then disconnect the output cable from the source Rotate the locking levers 90 degrees toward the front of the source to the unlocked position Figure 6 on page 9 Grasp the source housing with both hands and slowly pull away from the mass spectrometer until it clears the guide pins Af
20. bers see page 41 To contact Technical Support Phone 800 532 4752 Fax 561 688 8736 E mail North and South America us techsupport analyze thermofisher com Other continents eu techsupport cmf thermofisher com Web site WWW proxeon com Address Thermo Fisher Scientific Edisonvej 4 DK 5000 Odense C Find software updates and utilities to download at mssupport thermo com To find local contact information for sales or service Go to www thermoscientific com wps portal ts contactus or Use the list on the following pages Note The country code is enclosed in square brackets The city code or area code is enclosed in parenthesis For countries other than the U S when you dial from within the specified country dial the 0 of the city code For countries other than Italy when you dial from outside the country do not dial the 0 of the city code EASY Spray lon Source User Guide xv Preface North America United States 1400 North Point Pkwy 10 West Palm Beach FL 33407 E mail us customer support analyze thermofisher com Phone nii 1 800 532 4752 F XL ci cedet deeg 1 877 373 4006 Canada 2845 Argentia Road Unit 4 Mississauga Ontario L5N 8G6 E mail us customer support analyze thermofisher com Phone 4444 1 800 530 8447 gre 1 905 890 9161 Europe Austria Wehlistrasse 27b A 1200 Wien E mail service sid austria thermofisher com Phone uc 43 0 1 333 50 34 0
21. ch is also called nanoESI nanoLC gradient separation EASY Spray lon Source User Guide 43 Glossary nanoES nanoLC gradient separation nanoESI nanoLC gradient separation Employs microscale capillary columns to separate the analytes in complex mixtures The sample is loaded onto a column using an injection valve or a gas pressure vessel The mixture components are then eluted by a solvent gradient and pumped through the emitter nanoESI NSI source The entire nanoelectrospray ion source nanoESI NSI spray current The flow of charged particles in the nanoESI NSI source The voltage on the NSI spray needle supplies the potential required to ionize the particles nanoESI NSI spray voltage The high voltage that is applied to the spray needle in the nanoESI NSI source to produce the NSI spray current as liquid emerges from the nozzle The NSI spray voltage is selected and set the NSI spray current varies nanospray ionization NSI See nanoclectrospray ionization nanoESI or NSI S source Seeion source static nanoelectrospray A device that performs continuous analysis of small analyte solution volumes over an extended period of time 44 EASY Spray lon Source User Guide Thermo Scientific LLL _ Index C camera connecting 15 light button and focusing wheel locations 16 removing 14 compliance FCC vii regulatory vii WEEE ix consumables 41 contacting us xv controller PCB description 4 power supply
22. ct your local Thermo Fisher Scientific field service engineer or go to www proxeon com easyspray Contents Spare Parts Consumables Spare Parts A B mixing venting tee eg addo RR rd dE AR dU ol duc endo Ad SC901 EASY Spray controller PCB with a 2 5 mm hex key ES230 EASY Spray controller power supply with power adapter cable ES231 EASY Spray tools emitter positioning tool and hex keys 1 5 mm and 3 mm E3232 Monitor and power supply unit sans a ce EGER RR a ES217 Video camera and power supply unit ici ek oaa ea Eque EROR RR ee ES216 Consumables EASY Spray column 15 cm x 75 pm ID PepMap C18 3 pm particles ES800 EASY Spray column 15 cm x 50 pm ID PepMap RSLC C18 2 um particles ES801 EASY Spray column 25 cm x 75 pm ID PepMap RSLC C18 2 um particles ES802 EASY Spray column 50 cm x 75 pm ID PepMap RSLC C18 2 um particles ES803 Thermo Scientific EASY Spray lon Source User Guide 4 CCC Nm Glossary AB CD EF GH JK LMNOPQOURSTUVWXYZ A API ion transfer tube A tube assembly that assists in desolvating ions that are produced by the ESI nanoESI NSI or APCI probe atmospheric pressure chemical ionization APCI A soft ionization technique done in an ion source operating at atmospheric pressure Electrons from a corona discharge initiate the process by ionizing the mobile phase vapor molecules A reagent gas forms which efficie
23. cting 15 video picture adjusting 17 nanoelectrospray advantages of 2 nanoESI see NSI mode nanoViper fittings using 22 NSI mode manual configuration LCQ Deca XP Max mass spectrometer 31 setting the spray voltage 33 NSI Source dialog box 33 P power connection location 4 power supply units connecting camera 15 controller 10 monitor 15 product requirements xii regulatory compliance vii replaceable parts 41 46 EASY Spray lon Source User Guide S safety standards vii signal intensity troubleshooting 40 source see EASY Spray ion source spray voltage readback value 39 setting the value 33 spray troubleshooting 39 T temperature control dial settings 4 display codes 22 display location 4 tools and supplies connecting the LC 19 troubleshooting 39 Tune Plus opening 32 V venting tee recommended LC connections 24 W WEEE compliance ix X x y and z coordinates column holder 12 Thermo Scientific
24. e by using the cameras focusing wheel Figure 13 on page 16 Thermo Scientific EASY Spray lon Source User Guide 17 O Connecting the LC to the EASY Spray Column Follow these procedures to connect the plumbing from the Thermo Scientific LC instrument to the EASY Spray column Contents Tools and Supplies Installing the EASY Spray Column for the First Time Using the nanoViper Fitting Connecting the LC to the EASY Spray Column Tools and Supplies Table 4 lists the required tools and supplies for connecting the LC plumbing configurations to the EASY Spray column Not all parts are required for each configuration Note The EASY Spray Ion Source Kit P N ES081 supplies the venting tee fittings and sleeves You are responsible for supplying the remaining parts and tools Table 4 Tools and supplies Sheet 1 of 2 Image Description Part number One and two column setups A B mixing venting tee stainless steel SC901 with three attached ferrules and nuts Sleeves PEEK 1 16 in OD IDEX F 233 Wrench open end 1 4 in Thermo Scientific EASY Spray lon Source User Guide 19 4 Connecting the LC to the EASY Spray Column Tools and Supplies 20 Table 4 Tools and supplies Sheet 2 of 2 Description Gloves lint free and powder free Part number Fisher Scientific 19 120 2947A small 19 120 2947B medium 19 120 2947C large 19 120 2947D X large Thermo Scientific
25. e screw beyond an angle of 90 degrees from where you felt the initial resistance IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting do not overtighten the fitting Connecting the LC to the EASY Spray Column This section describes how to connect the one or two plumbing lines from a nanoLC instrument to the EASY Spray column in either a one or two column configuration Table 4 on page 19 lists the required parts Note The EASY nLC series instruments identify the two solvent lines as Column Out and Waste In CAUTION Wear protective gloves when handling the solvent lines Follow one of these procedures Connecting an EASY nLC 1000 Instrument to the EASY Spray Column Connecting an EASY nLC II Instrument to the EASY Spray Column on page 26 Connecting an RSLCnano System to the EASY Spray Column on page 28 Connecting an EASY nLC 1000 Instrument to the EASY Spray Column Follow the appropriate procedure for a one or two column setup The two column setup includes a precolumn connected in the column out flow path to the venting tee Note Remove the nuts and ferrules from the venting tee To plumb a one column setup from an EASY nLC 1000 instrument To plumb a two column setup from an EASY nLC 1000 instrument on page 25 Thermo Scientific EASY Spray lon Source User Guide 23 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column To p
26. ed inside the housing can severely burn you as this area of the probe can reach 350 C 662 F In addition although the exterior surface of the housing and the exposed part of the probe fall below the limit of 70 C 158 F for accessible metal surfaces they too pose a burn hazard Therefore handle ALL parts of the probe and housing with extreme caution Before removing the API probe and housing allow the part to cool to room temperature approximately 20 minutes before you touch it Ifthe mass spectrometer connects to an LC system leave the solvent flow from the LC pump on while the probe cools to room temperature 2 Ifinstalled remove the optional ion sweep cone Figure 5 from the mass spectrometer by grasping the outer ridges of the ion sweep cone and pulling it off The spray cone is directly behind the ion sweep cone Figure 5 lon sweep cone removed from the mass spectrometer s spray cone lon sweep cone Spray cone shown is the offset orifice type for ESI H ESI and APCI 8 EASY Spray lon Source User Guide Thermo Scientific 2 Installing the EASY Spray Source Installing the lon Source onto the Mass Spectrometer To install the EASY Spray source onto the mass spectrometer 1 Follow the procedure To prepare the mass spectrometer on page 8 2 Rotate the locking levers toward the front of the source to the unlocked position right lever in Figure 6 Figure 6 Locking lever po
27. electrostatic discharge ESD especially when the lab environment is at the lower end of the relative humidity specification see page xii For additional guidelines refer to the Operating Environment chapter in the preinstallation requirements guide for your mass spectrometer Proceed methodically Always place the components on a clean lint free surface Never overtighten a screw or use excessive force Contents Tools and Supplies Replacing the Controller PCB Tools and Supplies Table 6 lists the required tools and supplies for the maintenance of the EASY Spray source Table 6 Tools and supplies Description Part number Controller PCB and 2 5 mm hex key ES230 Thermo Scientific EASY Spray lon Source User Guide 35 6 Maintenance Replacing the Controller PCB Replacing the Controller PCB If the controller PCB becomes damaged the actual temperature display might remain off and the EASY Spray column might not heat Follow this procedure to install a new controller PCB To replace the controller PCB 1 Follow the procedure To remove the source from the mass spectrometer on page 14 2 Using the 2 5 mm hex key remove the four hex socket head screws located on the bottom of the source Figure 12 on page 13 3 Slowly pull out the enclosure for the controller PCB Figure 26 IMPORTANT Ifyou remove the controller PCB too quickly or at an angle you might damage the PCB components or the internal
28. eve On the Column Out line insert the fittings into the venting tees side port Figure 16 on page 24 tighten the nut by hand until you feel resistance and then use the 1 4 in wrench to tighten the nut until firmly secured do not overtighten On the Waste In line insert the fittings into the venting tee s bottom port tighten the nut by hand until you feel resistance and then use the 1 4 in wrench to tighten the nut until firmly secured do not overtighten Connect the venting tee to the EASY Spray column to complete the one column setup Figure 17 on page 24 shows an example of a one column setup for the EASY nLC 1000 instrument which uses nanoViper fittings 26 EASY Spray lon Source User Guide Thermo Scientific 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column To plumb a two column setup from an EASY nLC Il instrument 1 Assemble and connect the Column Out line as follows a Remove the smaller fitting from the ZDV adapter union insert the Column Out line into the fitting and then slowly push the line forward until it extends past the fitting Figure 20 Figure 20 Column Out line with smaller fitting from the ZDV adapter union Smaller fitting P N F 126S for the adapter union b With the plug inserted into the ZDV adapter union s larger end insert the Column Out line into the union s smaller end and then tighten the smaller fitting until you feel res
29. fety standards replacement parts and additional components options and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives EMC Directive 2004 108 EC EMC compliance has been evaluated by Intertek Semko AB EN61326 1 2006 Low Voltage Safety Compliance This device complies with Low Voltage Directive 2006 95 EC and harmonized standard EN 61010 1 2001 FCC Compliance Statement THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES OPERATION IS SUBJECT TO THE FOLLOWING TWO CONDITIONS 1 THIS DEVICE MAY NOT CAUSE HARMFUL INTERFERENCE AND 2 THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION CAUTION Read and understand the various precautionary notes signs and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturing location see the label on the instrume
30. he Technical Publications Editor at techpubs lcms thermofisher com Thank you in advance for your help SURVEY Thermo Scientific EASY Spray lon Source User Guide xi Preface Product Requirements Table 1 lists the environmental requirements for the EASY Spray source The requirements for the mass spectrometer supersede these requirements Refer to the Operating Environment chapter in the preinstallation requirements guide for your Thermo Scientific mass spectrometer CAUTION The EASY Spray source must connect to a certified Thermo Scientific mass spectrometer which supplies high voltage capable of delivering a maximum of 8 kV and 100 pA If you connect the EASY Spray source to another type of mass spectrometer you might impair the protection provided by the equipment Table 1 EASY Spray source environmental requirements Parameter Specification Altitude Up to 2000 m 6562 ft Humidity Maximum relative humidity 80 for temperatures up to 31 C 88 F decreasing linearly to 50 relative humidity at 40 C 104 F Operating environment Pollution degree 2 indoor environment normally only nonconductive pollution Temperature Ambient temperature 15 27 C 59 81 F Contents of the EASY Spray lon Source Kit Table 2 lists the parts supplied in the EASY Spray Ion Source Kit P N ES081 For a list of replaceable parts see Chapter 8 Replaceable Parts Table 2 EASY Spray lon Source Kit P
31. ientific 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column 3 Connect the union to the EASY Spray column to complete the one column setup Figure 22 Figure 22 Example of a one column setup for the RSLCnano system direct injection d P EASY Spray L D column of e Viper union 20 um ID Autosampler 1 uL loop EASY Spray lon Source User Guide 29 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column 30 To plumb a two column setup from an RSLCnano instrument Connect the precolumn to ports 2 and 5 on one of the switching valves Figure 25 Insert the nanoViper fitting on the 20 pm ID nanoViper capillary into port 4 on the same switching valve Insert the fitting on the other end of the capillary into the Viper union tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn 45 90 degrees Do not tighten the fitting by more than a 1 4 turn Note See the caution statement on page 28 Connect the union to the EASY Spray column to complete the two column setup Figure 23 Figure 23 Fxample of a two column setup for the RSLCnano system preconcentration aa BAR BEER Wach solvent ie NCS 3500RS EASY Spray column Viper union Precolumn Column compartment with two switching
32. in Figure 1 maintains excellent spray stability to ensure efficient evaporation and ionization of the liquid samples the key to achieving the highest sensitivity at nano flow rates Key benefits of the EASY Spray source are as follows Simple reliable and robust design Effortless nano liquid chromatography nanoLC MS integration Better separation with zero dead volume ZDV connections Increased productivity with the temperature controlled EASY Spray column Figure 1 nanoLC MS system using the EASY Spray ion source camera and monitor Thermo Scientific a Thermo Scientific mass spectrometer EASY nLC series instrument with the EASY Spray ion source Contents Advantages of Nanoelectrospray Functional Description Thermo Scientific EASY Spray lon Source User Guide 1 1 Introduction Advantages of Nanoelectrospray Advantages of Nanoelectrospray The use of electrospray ionization ESI has evolved as a leading technique for generating intact gas phase ions from thermally labile polar analytes in solution In this technique ionization is induced by the use of an emitter a capillary tube or needle at a controlled distance from a counter electrode Direct current dc voltage is applied either to the needle or to the solvent to produce a strong electrical field at the emitter tip The electric field excites the ions in the solution as they leave the emitter tip This interaction results in electrohydrody
33. install on the mass spectrometer The source housing includes two locking levers two observation windows a position adjustable column holder and a controller printed circuit board PCB For installation instructions see Installing the Ion Source onto the Mass Spectrometer on page 8 Figure 2 EASY Spray source housing Opening for the camera Source housing Column holder Using the top and front observation windows you can view the tip of the emitter positioning tool while you move it into position To enhance your view of the tool and then later of the emitter connect the digital video camera and LCD monitor For instructions see Chapter 3 Connecting the LCD Monitor and Video Camera The column holder is mounted onto the front observation window and includes a position control knob Use the control knob to align the z axis front to back of the emitter positioning tool Figure 9 on page 11 with the mass spectrometer ion transfer tube To align the tool s x axis side to side and y axis position up and down use the controls that are built into the EASY Spray source housing For instructions see Adjusting the Emitter Tip Position on page 11 EASY Spray lon Source User Guide 3 1 Introduction Functional Description Controller PCB 4 EASY Spray lon Source User Guide The EASY Spray source contains a controller PCB Figure 3 that powers and controls the heat generated for the connected EASY Spray column The
34. istance c Remove the plug from the adapter union and then connect the adapter unions larger end to the appropriate end of the nanoViper precolumn Note Follow the recommended flow direction stated on the precolumn label d Insert the fitting at the other end of the precolumn into the venting tee s side port Figure 16 on page 24 and then tighten the fitting by an additional 1 8 to 1 4 turn 45 90 degrees Do not tighten the fitting by more than a 1 4 turn CAUTION Because the fittings seal against each other inside the union do not tighten them by more than 1 4 turn 90 degrees Overtightening the fittings can irreparably damage their sealing surfaces For additional information about nanoViper fittings see Using the nanoViper Fitting on page 22 2 Assemble and connect the Waste In line as follows a Attach a nut and ferrule to the Waste In line See step la and step 1b in To plumb a one column setup from an EASY nLC II instrument on page 26 b Insert the nut and ferrule fittings into the venting tee s bottom port tighten the nut by hand until you feel resistance and then use the 1 4 in wrench to tighten the nut until firmly secured do not overtighten 3 Connect the venting tee to the EASY Spray column to complete the two column setup Figure 21 Thermo Scientific EASY Spray lon Source User Guide 21 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column Figure
35. itor and Camera Figure 13 Camera and column installed on the EASY Spray source Camera light button Camera focusing wheel Hex socket head screw to secure the camera 4 On the back of the monitor make the following connections BNC adaptor to one of the Video IN ports Cameras yellow video connector to the BNC adaptor Output cord from the other power supply unit to the DC 12V IN socket CAUTION After completing the power supply connections route the output cords so that they are not a trip hazard 5 Place the monitor on top of the mass spectrometer near the source by using its support arm Make sure that the monitor is not too close to the edge 16 EASY Spray lon Source User Guide Thermo Scientific 3 Connecting the LCD Monitor and Video Camera Adjusting the Video Picture Adjusting the Video Picture Use this procedure to focus the picture from the video camera For additional information refer to the manuals that came with the monitor and camera S Ze To adjust the video picture 1 Follow the procedure To connect the monitor and camera on page 15 2 Turn on the monitor by pressing its POWER button The picture appears after a few seconds and is probably blurred If there is no picture press the monitors SOURCE button to change to the other video input channel 3 If the picture is too dark turn on the camera light by pressing its top light button Figure 13 on page 16 4 Adjust the pictur
36. l ScanMode Display Setup Diagnostics Help LG a z EE ze Bele M E la Dee EE 1 VELOS PRO Instrument is off H Y VN i No spectral data available For Help press FL NUM 1 31 2012 2 35 PM 32 EASY Spray lon Source User Guide Thermo Scientific 2 Click the NSI Source icon or choose Setup gt NSI Source to open the NSI Source p dialog box Figure 25 Figure 25 NSI Source dialog box Sheath Gas Flow Rate arb Aus Gas Flow Rate arb Sweep Gas Flow Rate arb Spray Voltage kW Spray Current pA Capillary Temp C S Lens RF Level ST 0 0 0 275 00 60 0 a e a e Y 2 e a e Y Actual 0 00 0 00 0 00 SS 0 00 0 00 OK Cancel Help 3 In the Spray Voltage kV box enter 1 90 Enter the absolute value Use 1 90 kV as the start value for the spray voltage If the intensity of the full scan spectrum is low gradually increase the spray voltage to improve the spectrum The recommended range for the spray voltage is 1 40 2 40 kV 4 Click OK Thermo Scientific EASY Spray lon Source User Guide 5 Configuring the Mass Spectrometer for NSI Mode Configuring the nanoESI NSI Source Parameters 33 ee MM Maintenance This chapter describes how to replace the controller PCB in the EASY Spray source For optimal results follow these guidelines when performing the procedure in this chapter Take precautions against
37. lder s position 2 Remove the protective cap on the end of the EASY Spray column Figure 14 3 With the cable on the bottom slowly push the tip of the EASY Spray column into the column holder opening until it clicks into place As the EASY Spray column moves forward into the source the protective cover over the emitter retracts to expose the emitter tip 4 Connect the cable to the front output heater socket Note Do not pull on the cable from the EASY Spray column Use the plug to connect and disconnect the column Thermo Scientific EASY Spray lon Source User Guide 21 4 Connecting the LC to the EASY Spray Column Using the nanoViper Fitting 5 Follow the appropriate procedure in Connecting the LC to the EASY Spray Column on page 23 6 Using the temperature control dial set the temperature for the EASY Spray column Table 5 lists the display readings that can appear on the actual temperature display Table 5 Readings for the actual temperature display Display Description EASY Spray column is not connected xXx Actual temperature while heating up gs Actual temperature stabilized BL Disconnect and reconnect power to the source If the display does not change you must replace the controller PCB See Replacing the Controller PCB on page 36 Using the nanoViper Fitting 22 For some of the LC instruments the plumbing connects to the EASY Spray column through several nanoVipe
38. lumb a one column setup from an EASY nLC 1000 instrument 1 On the Column Out line insert the nanoViper fitting into the venting tee s side port as shown in Figure 16 tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn Do not tighten the fitting by more than a 1 4 turn Figure 16 Recommended plumbing locations on the venting tee nanoViper example I Location for the Column Out line Location for the EASY Spray column Location for the Waste In line CAUTION Because the fittings seal against each other inside the union do not tighten them by more than 1 4 turn 90 degrees Overtightening the fittings can irreparably damage their sealing surfaces For additional information about nanoViper fittings see Using the nanoViper Fitting on page 22 2 On the Waste In line insert the fitting into the venting tee s bottom port tighten the fitting by hand until you feel resistance and then tighten the fitting by an additional 1 8 to 1 4 turn Do not tighten the fitting by more than a 1 4 turn 3 Connect the venting tee to the EASY Spray column to complete the one column setup Figure 17 Figure 17 Example of a one column setup with nanoViper fittings Column Out line EASY Spray column Waste In line 24 EASY Spray lon Source User Guide Thermo Scientific 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Co
39. lumn lt To plumb a two column setup from an EASY nLC 1000 instrument 1 Assemble and connect the Column Out line as follows a Insert the nanoViper fitting on the Column Out line into the Viper union tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn 45 90 degrees Do not tighten the fitting by more than a 1 4 turn Note See the caution statement on page 24 b Connect the other end of the union to the appropriate end of the nanoViper precolumn Note Follow the recommended flow direction stated on the precolumn label c Insert the fitting on the other end of the precolumn into the venting tee s side port Figure 16 on page 24 and then tighten as noted in step 1a 2 On the Waste In line insert the fitting into the venting tee s bottom port tighten the fitting by hand until you feel resistance and then tighten the fitting again by an additional 1 8 to 1 4 turn Do not tighten the fitting by more than a 1 4 turn 3 Connect the venting tee to the EASY Spray column to complete the two column setup Figure 18 Figure 18 Example of a two column setup with nanoViper fittings and a Viper union Column Out line EASY Spray column Bai Waste In line Tip When you install the two column assembly clear the One Column Setup check box in the EASY nLC device section on the Devices page of the Maintenance menu F
40. namic disintegration of the fluid generation of droplets and formation of an aerosol jet Conventional ESI employs flow rates from 1 pL min to 1 mL min Due to the high volume of liquid exiting the emitter a drying gas thermal heating or both are often required to expedite desolvation and droplet shrinkage A form of ESI that employs low flow rates of 10 1000 nL min is nanoelectrospray ionization nanoESI or NSI which is also known as nanospray ionization NSI NSI or nanoESI generally does not require a drying gas or thermal heating Compared with ESI NSI tolerates a wider range of liquid compositions including pure water As you lower the flow rate a lower volume of mobile phase passes through the emitter producing smaller aerosol droplets This makes NSI more effective than conventional ESI at concentrating the analyte at the emitter tip producing significant increases in sensitivity demonstrated by the signal response of the mass spectrometer Note The Thermo Tune Plus application uses the terms nanospray and NSI Functional Description This section describes the following principal components of the EASY Spray source and their functions Source Housing Controller PCB Controller Power Supply 2 EASY Spray lon Source User Guide Thermo Scientific Source Housing Thermo Scientific 1 Introduction Functional Description The EASY Spray source housing Figure 2 arrives assembled and is easy to
41. nt WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product See www thermoscientific com rohsweee for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country WEEE Konformitat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96 EC erf llen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann Mehr Information ber die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific ber die Verwerter und weitere Hinweise die n tzlich sind um die Produkte zu identifizieren die unter diese RoHS Anweisung fallen finden sie unter www thermoscientific com rohsweee Thermo SCIENTIFIC Conformite DEEE Ce produit doit tre conforme a la directive europ enne 2002 96 EC des D chets d Equipements Electriques et Electroniques DEEE Il est marqu par le symbole suivant Thermo Fisher Scientific s est as
42. nt damage to software loss of data or invalid test results or might contain information that is critical for optimal performance of the system Note Highlights information of general interest Tip Highlights helpful information that can make a task easier Table 3 lists additional caution specific symbols that appear in the EASY Spray Ion Source User Guide Table 3 Caution specific symbols and their meanings Chemical Hazardous chemicals might be present in the instrument Wear gloves when handling carcinogenic corrosive irritant mutagenic or toxic chemicals Use only approved containers and procedures for disposing of waste oil Electric Shock An electric shock hazard is present in the instrument Proceed with caution Hot Surface Allow heated components to cool before touching or servicing the instrument PPP Pb xiv EASY Spray lon Source User Guide Trip Hazard The power supply cords might present a trip hazard Be aware of the cords locations and secure them in place as needed Thermo Scientific Contacting Us Thermo Scientific Preface There are several ways to contact Thermo Fisher Scientific for the information you need To copy manuals from the Internet Go to mssupport thermo com agree to the Terms and Conditions and then click Customer Manuals in the left margin of the window lt To order consumable and spare parts Go to www proxeon com easyspray For part num
43. ntly produces positive and negative ions of the analyte through a complex series of chemical reactions atmospheric pressure ionization API Ionization performed at atmospheric pressure by using atmospheric pressure chemical ionization APCD electrospray ionization ESI or nanoelectrospray ionization nanoESI or NSI dynamic nanoelectrospray See nanoESI nanoLC gradient separation E electrospray ionization ESI A type of atmospheric pressure ionization that is currently the softest ionization technique available to transform ions in solution into ions in the gas phase Thermo Scientific H H ESI Heated electrospray ionization H ESD a type of atmospheric pressure ionization converts ions in solution into ions in the gas phase by using electrospray ionization ESI in combination with heated auxiliary gas ion source A device that converts samples to gas phase ions ion sweep cone A removable cone shaped metal cover that fits on top of the API ion transfer tube and acts as a physical barrier to protect the entrance of the tube nano liquid chromatography nanoLC Liquid chromatography with typical flow rates of 10 1000 nL min and 10 150 pm diameter columns nanoelectrospray ionization nanoESI or NSI A type of electrospray ionization ESI that accommodates very low flow rates of sample and solvent on the order of 1 20 nL min for static nanoelectrospray or 100 1000 nL min for dynamic nanoelectrospray whi
44. o on Thermo Scientific EASY Spray lon Source User Guide 39 7 Troubleshooting Table 7 EASY Spray source problems causes and solutions Sheet 2 of 2 Problem The signal intensity is weak Cause The parameters for the LC MS or both might need adjusting Solution Try these solutions e Verify that the EASY Spray column is correctly positioned that is clicks on insertion Check the LC method and MS tune method parameters Runa known standard to check the sensitivity The mass spectrometer s spray cone and ion transfer tube are dirty Clean both the spray cone and ion transfer tube For instructions refer to the mass spectrometer s documentation Heater issues The column is not heated or The actual temperature display is blank The heater is off or there might be a loose connection or a Try these solutions Turn on the heater by selecting a temperature setting damaged PCB Check all of the connections for the controller power supply unit from the electrical wall outlet to the EASY Spray source See Replacing the Controller PCB on page 36 The EASY Spray column is Replace the EASY Spray column damaged 40 EASY Spray lon Source User Guide Thermo Scientific EM Replaceable Parts This chapter lists the consumable and replaceable parts for the EASY Spray source and their corresponding part numbers To order any of these parts conta
45. or instructions refer to Setting Up the Properties for the HPLC Device in Chapter 4 of the EASY nLC 1000 User Guide When the One Column Setup check box is clear not selected valve W on the EASY nLC 1000 moves to position 1 6 during the sample loading step Excess solvent A exits the venting tee and passes through valve W to the waste beaker Thermo Scientific EASY Spray lon Source User Guide 25 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column Connecting an EASY nLC Il Instrument to the EASY Spray Column Follow the appropriate procedure for a one or two column setup The two column setup includes a precolumn connected in the column out flow path to the venting tee Note Remove the nuts and ferrules from the venting tee 4 To plumb a one column setup from an EASY nLC II instrument To plumb a two column setup from an EASY nLC II instrument To plumb a one column setup from an EASY nLC II instrument Attach the nuts and ferrules to the Column Out and Waste In lines as follows a Insert one of the lines into a sleeve and then slowly push it forward until the line is parallel with the end of the sleeve b Slide the nut and then the ferrule onto the sleeved line until the sleeve extends past the ferrule Figure 19 c Repeat step la and step 1b with the second line Figure 19 LC line with sleeve ferrule and nut m Nut E Ferrule e Sle
46. pply unit Figure 4 on page 5 3 Plug the power supply unit into an electrical wall outlet 4 Connect the power adapter cable to the input power socket located on the bottom right side of the source Figure 12 on page 13 as follows a Align the red dot on the plug with the red dot on the socket which is on the right side of the socket Figure 8 Figure 8 Power connection for the controller PCB Red dot on the socket K Red dot on the plug 24VDC 20w b Push the plug upward into the socket CAUTION After completing the power supply connections route the output cord so that itis not a trip hazard 10 EASY Spray lon Source User Guide Thermo Scientific 2 Installing the EASY Spray Source Adjusting the Emitter Tip Position Adjusting the Emitter Tip Position Before installing the EASY Spray column use the emitter positioning tool Figure 9 and 3 mm hex key to adjust the position of the column holder Figure 10 shows the emitter positioning tool touching the mass spectrometer s ion transfer tube which then correctly provides a 3 mm gap for the EASY Spray column s emitter Figure 9 Emitter positioning tool Figure 10 Gap positions in front of the mass spectrometer s ion transfer tube 0 mm gap between the ion transfer tube and the emitter positioning tool B Emitter positioning tool ee Mass spectrometer spray cone
47. r fittings such as the one shown in Figure 15 Although these fittings can withstand ultra high performance LC UHPLC backpressures up to 1034 bar 15 000 psi they are fingertight fittings which require only very small torques to seal Therefore you must follow the procedure below to avoid damage by overtightening Figure 15 nanoViper fitting PEEK sealing surface Removable knurled tightening tool Screw ceo lt To use a nanoViper fitting 1 Insert the nanoViper fitting into the target port and slowly rotate the screw clockwise until you feel resistance 2 Using the black knurled fitting tool tighten the screw clockwise to an angle of 0 45 degrees 1 8 turn 3 Start operating the system at the desired working pressure and check the backpressure EASY Spray lon Source User Guide Thermo Scientific 4 Connecting the LC to the EASY Spray Column Connecting the LC to the EASY Spray Column 4 If the backpressure is too low check the system for leaks For instructions refer to the documentation for the LC instrument 5 If the backpressure continues to be too low return the system to atmospheric pressure IMPORTANT To extend the lifetime of the nanoViper fittings open and close the connections at only atmospheric system pressures Opening and closing connections at high system pressures can reduce the lifetime of the fittings 6 Tighten the screw by as much as an additional 45 degrees Do not turn th
48. sitions Locked position Unlocked position f K J ht Top observation window J 3 Align the two pin holes on the back of the source with the guide pins on the mass spectrometer ion source mount assembly Figure 7 Note Your mass spectrometer might have a slightly different mount assembly ion sweep cone or both Figure 7 lon source housing connection High voltage HV connector Guide pins on the mass spectrometer s Guide pin holes on the back of the ion source mount assembly EASY Spray source housing Thermo Scientific EASY Spray lon Source User Guide 9 2 Installing the EASY Spray Source Connecting the Controller Power Supply 4 Carefully press the source onto the mass spectrometer 5 Rotate the locking levers 90 degrees toward the top window to the locked position left lever in Figure 6 on page 9 The locked levers are now parallel with the front of the mass spectrometer Connecting the Controller Power Supply CAUTION To avoid an electric shock always use the detachable input plug provided with the power supply unit that is suitable for your country or region lt To connect the controller power supply unit 1 Turn the temperature dial on the EASY Spray source to the OFF position 2 If not already connected connect the detachable input plug and power adapter cable to the power su
49. soci avec une ou plusieurs compagnies de recyclage dans chaque tat membre de l union europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Fisher Scientific ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la d tection des substances sujettes la directive RoHS sont disponibles sur www thermoscientific com rohsweee LL m Preface The EASY Spray Ion Source User Guide describes the hardware components and provides installation and configuration procedures for the Thermo Scientific EASY Spray ion source Use the EASY Spray ion source with the following Thermo Scientific mass spectrometers MSs for nanoelectrospray commonly referred to as nanoES or nanospray analysis Exactive Q Exactive e Orbitrap Series e LTQ Series TSQ Series LCQ Deca XP Max For information about your specific mass spectrometer refer to the user documentation provided with your instrument Contents Product Requirements Contents of the EASY Spray Ion Source Kit e Safety and Special Notices Contacting Us To suggest changes to the documentation Complete a brief survey about this document by clicking the button below If you have a printed copy of this document fill out a reader survey online at www surveymonkey com s PQM6P62 or send an e mail message to t
50. spain thermofisher com Phone 34 914 84 59 65 Fax 34 914 84 35 98 Thermo Scientific Europe continued Sweden Norway and Finland Pyramidbacken 3 S 141 75 Kungens Kurva Stockholm Sweden E mail service sid nordic thermofisher com Phone 46 0 855 64 68 20 Fax stan cit 46 0 855 64 68 08 Switzerland Neuhofstrasse 11 4153 Reinach E mail service sid ch thermofisher com Phone 41 617 16 77 40 Fax cu tenaces 41 617 16 77 20 United Kingdom Stafford House 1 Boundary Park Boundary Way Hemel Hempstead Hertfordshire HP2 7GE E mail service sid hemel gthermofisher com Phone 44 0 870 241 1034 ig cp bici 44 0 144 223 3667 Thermo Scientific Preface Australasia and Asia Australia P O Box 9092 5 Caribbean Drive Scoresby VIC 3179 E mail analyze au thermofisher com Phone 61 39757 4300 E ees 61 9763 1169 Japan C 2F 3 9 Moriya cho Kanagawa ku Yokohama 221 0022 E mail analyze jo thermofisher com PRONE 81 45 453 9100 O 81 45 453 9110 P R China 7th Floor 7F Tower West Younghe Plaza No 28 Andingmen East Street Beijing 100007 E mail analyze cn thermofisher com Phone free lines 800 810 5118 400 650 5118 Fax feats eins 86 108 837 0548 EASY Spray lon Source User Guide xvii LLL m Introduction The Thermo Scientific EASY Spray ion source shown
51. ter you remove the EASY Spray source from the mass spectrometer place it in its original shipping box The EASY Spray source does not require cleaning 14 EASY Spray lon Source User Guide Thermo Scientific M Connecting the LCD Monitor and Video Camera Follow these procedures to connect the LCD monitor and digital video camera and to adjust the video picture The only tool required is the 1 5 mm hex key Contents e Connecting the Monitor and Camera e Adjusting the Video Picture Connecting the Monitor and Camera To enhance your view of the emitter tip install the LCD monitor and digital video camera that are provided in the EASY Spray Ion Source Kit see Table 2 on page xii lt To connect the monitor and camera 1 Follow the procedure To install the EASY Spray source onto the mass spectrometer on page 9 2 Plug the two power supply units into electrical wall outlets 3 Install the camera as follows a Insert the camera into the opening above the top observation window with the focusing wheel facing toward you Figure 13 b Using the 1 5 mm hex key tighten the hex socket head screw on the left side of the top observation window Figure 11 on page 12 until it touches the camera do not overtighten the screw c Connect the camera s black power connector to its power supply unit Thermo Scientific EASY Spray lon Source User Guide 15 3 Connecting the LCD Monitor and Video Camera Connecting the Mon
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