OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION OF
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1. D Electronic 9 Instruments for the Life Sciences n Qa e wade 70 OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION OF THE ISO STIM 01M STIMULUS ISOLATION UNIT FOR EPMS SYSTEMS s0 STIM 01M OMM AMPLITUDE x D IV x CURRENT RANGE OUTPUT MODE VOLTAGE BIPOLAR ON w CURRENT BIPOLAR OFF z Q OFFSET 9 Q BALANCE C CARCOMP ve 2 GATE TIL SIGNAL INPUT STIMULUS BOLED OUTPUT INDICATOR CAUTION HIGH VOLTAGE UN npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com ISO STIM 01M User Manual Table of Contents Iris OU LATIONS T E 3 2 Special Safety Notice for High Voltage Instrumentts cccccccccccsssssseeeceeeeeeeaeeeseeeeeeees 4 3 EPMS 07 Modular Plug In System deett pe en ntt ots tu dara rave in a den ases cu deduce atels 5 3 1 General System Description Operation essssssoeeessssssssecerrssssssssceerrssssssseerressssseee 5 5 2 EPM SO HOUS N e A E EN 5 I PPMS EO 7 ar OU SIS aa e E E A 5 JA PWR O PEINT 5 23s DYSE OrOUndIn Ta Fal epa Doe tuto Puerta Eus pa lont us is cc Dolo tu d dud dS 6 DOr TC UIDI Mri 6 4 ISO S TIM 01M Stimulus Isolation U nits ue cristo heteaiaeacii ah arial 7 du SV Ste CSC ON aorta hose a E atau nice eMe 7 4 2 Description of the Front Panel iisudenassaxcsecesandenaasesasinsiunedeeadenuatedevandeetacesaake2. The amplitude of the pulse is set by the AMPLITUDE thumb wheel switch The reading of this switch is multiplied by 0 1 V in VOLTAGE OUTPUT MODE In CURRENT OUTPUT MODE the reading is multiplied by the factor set with the CURRENT RANGE switch For example 250 at the thumb wheel switch will give 25 V or 250 uA CURRENT RANGE switch in position x1 uA at the output plugs depending on the setting at the OUTPUT MODE switch If the CURRENT RANGE switch is in position x10 uA 250 at the thumb wheel switch will give 2 5 mA at the output plugs DIRECT INPUT MODE The signal fed into the SIGNAL INPUT connector is transformed into the isolated output signal and amplified by a factor of ten OUTPUT MODE VOLTAGE i e an input of 1 V will lead to an output stimulus of 10 V In CURRENT OUTPUT MODE the amplitude of the output stimulus is again dependent on the setting the CURRENT RANGE switch With the CURRENT RANGE switch in position x1 uA an input voltage of 1 V will result in an output stimulus of 100 uA and with the CURRENT RANGE switch in position x10 uA the output current will be 1 mA The polarity switch must be in 0 position The frequency of the input signal should not exceed 4 kHz ANALOG INPUT MODE The signal fed into the SIGNAL INPUT connector will be isolated from earth and amplified OUTPUT MODE voltage as in the DIRECT INPUT MODE The trigger level is set to 300 mV i e the input signal must have an amplitude of 300 mV or more If th
3. rackmount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel 2 The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 3 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing V EPMS 07 cabinet with built in power supply Mains cord V Fuse2A 1 A slow Front covers 3 In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing 3 3 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow Front covers SW NNN SS The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the power supply 3 4 PWH 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing is connected by a 6 pole cable from the one of the three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 1 Fig
4. General safety regulations for operating electrical devices are to be considered AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet disconnect mains power plug Disconnect mains power plug when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care This can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e a
5. the input signal but is triggered by the input signal itself and the duration of the output stimulus is set at the ISO STIM 01M Two LEDs to indicate if the amplifier is 10 below its positive or negative limit limit is 100 V or 10 mA respectively and one LED to indicate the application of a voltage or current stimulus Three trim pots allow compensation of electrode artifacts CAP COMP OFFSET and for electronic offsets BALANCE SPECIAL WARNING THIS INSTRUMENT HAS A HIGH VOLTAGE OUTPUT PINS MARKED ISOLATED OUTPUT CAUTION HIGH VOLTAGE Do not touch these pins nor bare wires connected to this pins Always turn power off if you manipulate devices connected to these pins version 3 8 page 7 ISO STIM 01M User Manual 4 2 Description of the Front Panel D ISO STIM 01M npi O AMPLITUDE x0 1V x CURRENT RANGE OUTPUT MODE m VOLTAGE BIPOLAR ON 9 a a CURRE BIPOLAR OFF X1QUA x UA x0 111A CURRENT RANGE ANALOG MAN TRIGGER CHASSIS RANGE DIRECT TIME BASE INPUT MODE 9 O 9900 0 o Oo GATE TIL SIGNAL INPUT STIMULUS ISOLATED OUTPUT INDICATOR CAUTION HIGH VOLTAGE O O Figure 4 ISO STIM 01M front panel view 1 2 OUTPUT MODE switches 1 BIPOLAR ON BIPOLAR OFF switch erreg Two position switch for selecting the signal OUTPUT mode Upper position BIPOLAR ON Lower position BIPOLAR OFF BIPOLAR OFF version 3 8 page 8 ISO STIM 01M User
6. ANALOG INPUT BNC Output voltage will occur if the input voltage is higher than 300 mV this is the internal analog trigger level and only for the desired time here 300 us The output signal can be varied by using other input amplitude values and or several time settings Caution If you are working in CURRENT MODE realize that the input resistance of an oscilloscope is normally 1 MQ e g an oscilloscope connected to the outputs is a 1 MQ load for the current source producing an output signal that is measured version 3 8 page 16 7 Technical Data Input operating modes Function modes Source ranges Time range factor Amplitude potentiometer Time potentiometer Input voltage range Input impedance Output Output voltage Output current version 3 8 ISO STIM 01M User Manual DIRECT ANALOG TRIGGER TTL TRIGGER selectable with toggle switch voltage source current source selectable with toggle switch 100 uA 1 mA 10 mA current source range selectable with 3 position rotary switch 100 V voltage source x10 us x100 us x1 ms selectable with toggle switch time range up to 99 ms 3 digits 10 V amplified internally with gain of 10 voltage source or scaled with source range switch current source polarity selectable with toggle switch 2 digits 0 99 as multiplication factor selectable 10 V amplified internal with gain of 10 10 kQ monopolar or bipolar selected by toggle switc
7. duration is not defined and should not be set 17 18 OVER LEDs LEDs indicating when the isolator exceeds it s linear range limit 90 V or 9 mA respectively version 3 8 page 11 ISO STIM 01M User Manual Switch for setting the polarity of the output signal in GATE TTL INPUT MODE In 0 position the internal stimulus generation is disabled Note In ANALOG or DIRECT INPUT MODE this switch must be in 0 position 20 CURRENT RANGE switch ieee Three position rotary switch to set the current range of the build in timing unit Available ranges 0 1 99 9 uA lower position 1 999 uA middle position 10 9999 uA upper position AMPLITUDE Ten turn potentiometer for setting the AMPLITUDE of the output ATES sional Range in VOLTAGE mode 0 1 99 9 V Amplitude in CURRENT mode is the reading of this potentiometer multiplied by the factor set with switch 20 version 3 8 page 12 ISO STIM 01M User Manual 5 Modes of Operation GATE TTL INPUT MODE In this mode the output signal is generated by the build in timing unit It is triggered either manually via the MANUAL TRIGGER switch or via a TTL pulse at the GATE TTL BNC connector The duration of the pulse is set at the DURATION thumb wheel switch and the 3 position range switch below The duration time is determined by reading of the thumb wheel switch multiplied by the factor set via the range switch The minimum duration is 10 us the maximum is 99 ms
8. realize that a connection is also possible through the protective ground connections between oscilloscope and function generator most mains supplied oscilloscopes have a connection between ground and protective earth Thus we recommend to use a battery driven oscilloscope for test measurements to avoid unwanted grounding Now the OUTPUT MODE switch is set in position CURRENT CURRENT RANGE switch set to x1 uA and the amplitude of the input signal is 2V The max signal at the isolated output plugs has to be 20 V according to the current of 200 uA flowing through the load resistance of 100 kQ 6 2 TTL trigger mode Settings INPUT MODE switch is in position GATE TTL OUTPUT MODE switch in position VOLTAGE The TTL output of the function generator has to be connected to the GATE TTL BNC the SIGNAL INPUT BNC is not connected The output voltage will be preset from the value dialed at the AMPLITUDE potentiometer and the polarity from the position of the corresponding toggle switch Remember at any time Ohm s law to realize what potentials should be generated theoretically the maximum output voltage of the system is 100V 6 3 ANALOG trigger mode Settings INPUT MODE switch is in position ANALOG TRIG OUTPUT MODE switch in position VOLTAGE Toggle switch for DURATION range in position 100 us TIME potentiometer dialed on value 3 Input voltage from a function generator has to be positive referring to ground value 5 V and connected to the
9. rear panel of the instrument see Figure 3 Important Always adhere to the appropriate safety measures Figure 3 Rear panel connectors of the EPMS E 07 3 6 Technical Data 19 rackmount cabinet for up to 7 plug in units Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep EPMS 07 Power supply 115 230 V AC 60 50 Hz fuse2 A 1 A slow 45 60 W EPMS E 07 External power supply for EPMS B 115 230 V AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions of External power supply W x D x H 225 mm x 210 mm x 85 mm version 3 8 page 6 ISO STIM 01M User Manual 4 ISO STIM 01M Stimulus Isolation Unit 4 1 System Description The ISO STIM 01M stimulus isolation unit is a plug in unit for the np1 EPMS 07 modular system It is designed for application of extracellular stimuli e g in brain slices The output signal 1s optically separated from ground and can either be a constant voltage up to 100 V or a constant current up to 10 mA The ISO STIM 01M has a built in timing unit with adjustable pulse length amplitude and polarity The ISO STIM 01M has three modes of operation 1 In the DIRECT input mode the output signal follows exactly the input signal 2 In the GATE TTL input mode the output signal is generated by the build in timing unit and triggered either by a TTL trigger at the GATE TTL connector or manually with the MAN TRIGGER switch 3 In the ANALOG input mode the output signal follows like in direct mode
10. Manual 2 VOLTAGE CURRENT switch Two position switch for selecting the isolator OUTPUT mode Upper position VOLTAGE Lower position CURRENT Important If the CAP COMP 5 is overcompensated in CURRENT MODE the stimulus isolator will ring and not work properly 3 OFFSET trim pots OFFSET C trim pot T Trim pot to compensate for the CURRENT OFFSET of the stimulating electrode in all input modes It is recommended to compensate the offsets only in a completely warmed up condition 1 e after 30 minutes warm up time C V 0 NS OFFSET V trim pot Trim pot to compensate for the VOLTAGE OFFSET of the stimulating electrode in TTL mode It is recommended to compensate the offsets only in a completely warmed up condition i e after 30 minutes warm up time Compensation procedure is the same for current and voltage in the TTL MODE 4 BALANCE trim pot Trim pot for compensating for baseline differences of the stimulus when switching from DIRECT to GATE TTL mode or vice versa Even if no stimulus is applied in DIRECT MODE or ANALOG MODE the baseline of the stimulus isolator may not be zero Using this trim pot the stimulus output can be balanced to zero OFFSET BALANCE adjustment First the voltage source OFFSET is compensated in TTL mode using the VOLTAGE OFFSET trim pot 18 The same procedure is repeated in TTL mode for current source OFFSET if the ISO STIM 01D is in CURRENT mode using the CURRENT OFFSET tri
11. e trigger level is exceeded the input signal is transformed to the isolated output plugs and the output stimulus follows the input signal as long as set by the DURATION thumb wheel Figure 5 gives some examples how the different input modes work Please note that this figure does not show original recorded data It s for illustration only version 3 8 page 13 ISO STIM 01M User Manual BIPOLAR The isolator is capable to generate biphasic stimuli by switching the stimulus mode switch to BIPOLAR ON In this mode the stimulus that is set by the user will be followed by a second stimulus with the same amplitude and duration but with reverse polarity There is no delay between first and second part of the stimulus For instance if the user sets a positive stimulus of 1 V amplitude and 100 us duration this stimulus will be followed immediately by a negative stimulus of 1 V and 100 us duration OPERATION IN THE 10 mA max RANGE According to Ohm s law with 100 V voltage 10 mA current can be achieved only if the resistance of the stimulus electrode is not higher than 10 kQ Otherwise the current will be less The output is short circuit protected with a 1 KQ resistor This implicates that when using low resistance stimulation electrodes with high current flow there is a voltage drop at this resistor CAUTION This means that in voltage operation mode the output swing is reduced due to the voltage divider between the electrode resis
12. fter internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient 5 HANDLING Please protect the device from moisture heat radiation and aggressive chemicals 6 SPECIAL WARNING THIS INSTRUMENT HAS A HIGH VOLTAGE OUTPUT PINS MARKED ISOLATED OUTPUT CAUTION HIGH VOLTAGE Do not touch these pins nor not insulated wires connected to these pins Always turn power off if you manipulate devices connected to these pins version 3 8 page 3 ISO STIM 01M User Manual 2 Special Safety Notice for High Voltage Instruments 1 2 3 4 5 6 DANGER HIGH VOLTAGE RISK OF ELECTROCUTION Observe extreme caution when working with this instrument Always connect high voltage power supplies to protective earth Do not touch connections unless the instrument is turned off and the capacitance of both the load and power supply are earthed Allow adequate time for discharge of internal capacitance of the power supply Do not ground yourself or work under wet or damp conditions Servicing should be only done by qualified personnel aware of the hazards If in doubt return to supplier for servicing version 3 8 page 4 ISO STIM 01M User Manual 3 EPMS 07 Modular Plug In System 3 1 General System Description Operation 1 The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19
13. h 100 V max isolated 10 mA max isolated page 17
14. m pot 17 If these baselines are set the isolation amplifiers may still produce a signal in ANALOG or DIRECT mode even if no stimulus is applied This offset signal is balanced to zero using the BALANCE trim pot 5 CAP COMP trim pot Potentiometer that compensates for the input capacitances in isolated c cowe current output operation Capacity compensation is achieved by turning the potentiometer with a small screw driver clockwise until the current signal at the oscilloscope is as square as possible o CARCOMP Important If the CAP COMP is overcompensated in CURRENT MODE the stimulus isolator will ring and not work properly version 3 8 page 9 ISO STIM 01M User Manual 6 CHASSIS jack Jack linked to the CHASSIS The green banana connector of the supplied cable is connected here 7 8 ISOLATED OUTPUT jacks Jacks providing the ISOLATED OUTPUT signal The polarity of the input of the 0 switch 19 Figure 4 respectively is not changed 1 e the red plug is positive if the output polarity switch is set to or the input signal is positive This signal is completely isolated from earth The black jack serves as reference point for the isolated output signal The black ea ena Ves VNTR banana connector of the supplied cable is connected to the black jack and the red to the red jack WARNING THIS INSTRUMENT HAS A HIGH VOLTAGE OUTPUT UP TO 100 V Do not touch these pins or bare wires connected to the
15. se pins Always turn power off if you manipulate devices connected to these pins 9 10 STIMULUS INDICATOR 9 STIMULUS INDICATOR LED Yellow LED indicating that a stimulus is applied 10 STIMULUS INDICATOR BNC Need BNC connector providing a TTL signal whenever a stimulus is applied 11 MAN TRIGGER button MESE Button for triggering the isolator in GATE TTL INPUT MODE manually 12 SIGNAL INPUT BNC BNC connector for the analog INPUT SIGNAL The signal is transformed into the isolated output signal and amplified by a factor of ten see also chapter 5 version 3 8 page 10 ISO STIM 01M User Manual 13 GATE TTL BNC BNC connector for triggering the stimulus isolator in GATE TTL INPUT MODE see also chapter 5 GATE TIL 14 INPUT MODE switch ANALOG Three position switch to set the operating mode of the stimulus isolator either to ANALOG to GATE TTL or to DIRECT INPUT MODKE see also JI BEA chapter 5 D B oec DIRECT INPUT MODE 15 TIME BASE RANGE switch Three position switch to set the time base of the built in timing unit Available ranges 10 99 us lower position 100 999 us middle position 1 99 ms upper position 16 DURATION potentiometer DURATION 2 digits digital potentiometer to set the stimulus duration in GATE TTL INPUT MODE The stimulus duration is the reading of this potentiometer 5 multiplied by the factor set with switch 16 Note Setting of 00 as
16. tance and the protection resistor version 3 8 page 14 ISO STIM 01M User Manual Gate TIL Mode input at GATE TIL BNC Gate TIL input j Ll L if DURATION is set to 20us V isolated output Pulse amplitude is set by AMPLITUDE potentiometer 20us Analog input input at SIGNAL INPUT BNC mode V signal input V isolated output BENE if DURATION is set Z us to 20us Pulse amplitude is set by signal input Direct mode input at SIGNAL INPUT BNC V signal input V isolated output amplification of the output signal is not shown Figure 5 Modes of operation illustration only version 3 8 page 15 ISO STIM 01M User Manual 6 Test Procedures For the following test procedures a function generator is required The frequency for testing should be around 100 Hz waveform is triangle The value of the test pulse amplitude is always described detailed in each of the following steps In this test condition a load resistor of 100 kQ is connected to the output plugs red and black plugs 6 1 DIRECT mode Settings INPUT MODE switch is in position DIRECT OUTPUT MODE switch in position VOLTAGE Analog input voltage from a function generator is 2 V and connected to the SIGNAL INPUT BNC Max output voltage has to be 20 V Caution If you measure the output signal with an oscilloscope take care that the function generator has not the same ground connection because of the isolation of the output It is important to
17. twandeecdebnaaients 8 2 Modes OF CODO Atl OU eine ie ese a Ata p teu anda adnan veces M IU MP ADAM MU DES 13 GATE TTEINPUT MODE s udetintuser d ctia mestecdi annie tuse soe meato nat ccdactannte ase eecae 13 DIREC Foal Wis el Ys DB arenes epee eer ae mem ee ee eee ee Ce ee ee ere 13 ANALOG INPUT MODE ranni ink iat onenatiea eked 13 BIPOLAR qc 14 OPERATION IN THE 10 mA max RANGE eee 14 6 Test Procedures uidi bietet iud darte bue bi tain a a v quie teeta M bm re tub sue ih 16 om EM DIRECT un 1 16 6 25 EAC doen db tones dtes tasers tare tat tinea E Diettdii ded etaim 16 Oc ZEN AE OG Cr O vides eek ere ped d um c tes putt dide 16 Te E eiiis DNE UU UU T UU UU 17 version 3 8 page 2 ISO STIM 01M User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic expressively disclaims any warranties for such purpose Equipment supplied by npi electronic shall be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 GENERAL This system is designed for use in scientific laboratories and should be operated by trained staff only
18. ure 3 A POWER LED indicates that the PWR 03D is powered on see Figure 1 Power switch voltage selector and fuse are located at the rear panel see Figure 2 Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected version 3 8 page 5 ISO STIM 01M User Manual Figure 1 PWR 03D front panel view Figure 2 PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only 3 5 System Grounding EPMS 07 The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration a EPMS E 07 e The 19 cabinet 1s connected to the PROTECTIVE EARTH connector at the rear panel The chassis is linked to protective earth only if the PWR 03D is connected It can be connected also to the SYSTEM GROUND SIGNAL GROUND on the
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