UltiMate 3000 SRD-3x00 Solvent Rack Operating Instructions, Rev
Contents
1. 3 4 1 Connecting the SRD to an UltiMate 3000 System Pump Locate the connection cable in the accessories kit for the SRD and connect the 15 pin D Sub port on the rear panel of the SRD with the Solvent Rack port on the rear panel of the pump To ensure trouble free operation use only the cable shipped with the instrument When the SRD is connected to the pump the solvent rack is powered from the pump The SRD must not be connected directly to the main power supply For information about the pin assignment for the 15 pin D Sub connector see page 53 3 4 2 Connecting the SRD to the Main Power Supply Optional The SRD needs to be connected to the main power supply only if it is not operated from an UltiMate 3000 system pump For this scenario an external power supply part no 6510 0004 is available from Dionex as an option for the SRD Use the external power supply unit to connect the DC input on the rear panel of the SRD to a power source that is connected to a true ground No manual adjustment is required to adapt the line voltage to local voltage requirements A Important Do not connect the external power supply unit when the SRD is connected to an UltiMate 3000 system pump section 3 4 1 A Important N utilisez pas d alimentation lectrique externe si l instrument est raccord un pompe du syst me UltiMate 3000 chapitre 3 4 1 Operating Instructions Page 19 UltiMate 3000 Series SRD 3x00 Solvent Racks2. Page 20 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 4 Preparation for Operation Startup 4 1 Overview After you have unpacked positioned and connected the SRD described in sections 3 1 through 3 4 page 15 and following pages prepare the SRD for operation 1 Connect drain tubing section 4 2 2 Connect the solvent reservoirs and then connect the solvent lines to the degassing channels page 22 3 Select one of the following alternatives e To operate the SRD with the pump of the UltiMate 3000 System Connect the pump to the degassing channels page 25 e To operate the SRD stand alone Connect the degassing channels as required by your application 4 2 Connecting Drain Tubing To discharge liquid leaks that might have accumulated in the interior the SRD has a drain port at the bottom right of the instrument Fig 6 Drain port Direct liquid leaks to waste through the drain system of the UltiMate 3000 system by using the components from the drain kit The components required for connecting the drain tubing are shipped with the UltiMate 3000 pumps and the NCS 3x00RS but can be ordered also separately The drain kit part no 6040 0005 includes all required components and detailed installation instructions Operating Instructions Page 21 UltiMate 3000 Series SRD 3x00 Solvent Racks 4 3 Solvent Reservoirs All Solvent Racks are shipped with solvent reser
3. UltiMate 3000 Series SRD 3x00 Solvent Racks 1 2 Safety Information The CE Mark label and cTUVus Mark safety label on the instrument indicate that the instrument is compliant with the related standards 1 2 1 Symbols on the Instrument and in the Manual The table shows the symbols used on the instrument Alternating current Courant alternatif Refer to the Operating Instructions to prevent risk of harm to the operator and to protect the instrument against damage R f rez vous ce manuel pour viter tout risque de blessure l op rateur et ou prot ger l instrument contre tout dommage Label according to the Measures for Administration of the Pollution Control of Electronic Information Products China RoHS guideline Etiquette Measures for Administration of the Pollution Control of Electronic Information Products China RoHS WEEE Waste Electrical and Electronic Equipment label For more information see the WEEE Information section in the Installation and Qualification Documents for HPLC Systems binder Etiquette WEEE Waste Electrical and Electronic Equipment Pour plus d informations r f rez vous au chapitre WEEE Information dans le classeur Installation and Qualification Documents for HPLC Systems At various points throughout the manual messages of particular importance are indicated by certain symbols D Tip Indicates general information as well as information intended to optimize the perform
4. contact Dionex Service or your local distributor An RMA Return Material Authorization number is required in order to track your instrument Always use the original packaging and observe the packing instructions when shipping the SRD Shipping the SRD in anything other than the original packaging will void the warranty For more information see the warranty statement in the terms of sale For instructions on shutting down the unit see page 34 Operating Instructions Page 43 UltiMate 3000 Series SRD 3x00 Solvent Racks 7 2 Rinsing the Degassing Channels To avoid contamination of the degasser you should prepare fresh solvents clean the solvent lines and rinse the degassing channels at regular intervals Rinsing the degassing channels is especially important for the channel that degasses aqueous solvents for reverse phase chromatography Usually it is sufficient to rinse all channels with organic solvent Use a fresh bottle When you use water and acetonitrile or methanol it is usually sufficient to rinse the degassing channels once per week However adapt the rinsing intervals to the solvents in use In persistent cases for example reproducible ghost peaks in the chromatogram follow these steps l Install a backpressure capillary on the pump outlet The capillary should be appropriate for generating a backpressure of 20 to 30 MPa Rinse the degassing channels for 1 hour with 20 nitric acid at the flow rate
5. for example as shown in Fig 4 and interconnecting them on the rear panel as shown in Fig 5 However the arrangement of the system modules depends on the application CN Solvent Rack eMac 3000 e Pump 3700 732 5 000 Autosampler Column compartment Detector Fig 4 Module arrangement for an UltiMate 3000 system example If the solvent rack is part of an UltiMate 3000 RSLCnano system or Proteomics MDLC system the related system manual provides information about how to arrange the modules and set up the system for these applications Operating Instructions Page 17 UltiMate 3000 Series SRD 3x00 Solvent Racks Solvent Rack Pump here RS pump Autosampler Column compartment Detector here DAD 3000 MWD 3000 Power Fig 5 Example for the rear panel connections on an UltiMate 3000 system Apart from the Solvent Rack all modules of the UltiMate 3000 system can be connected separately to the Chromeleon computer by using the USB port on the rear panel of the instrument However Dionex recommends interconnecting all modules and then connecting the system to the Chromeleon computer by using only one connection For systems with a DAD 3000 RS or MWD 3000 RS you can use only the hub on the detector for the connection For systems with a VWD 3x00 RS only use the hub on the pump Page 18 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 3 4 Connecting the SRD
6. 0047 Included in 6270 0013 6268 0115 6268 0110 Included in Included in 6000 0042 TE E Quantity Part No in the kit GH 6000 1006 Included in 6000 0047 Included in 6270 0013 2 Included in Included in 2 2 2 Included in 6000 0042 2 2 6268 0115 6268 0110 ane amon ooo Towns pene a oo CT 1 0 1 Page 50 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 9 2 Optional Accessories Description Part No Remarks Drain kit for UltiMate 6040 0005 The drain kit includes all required components and detailed 3000 systems installation instructions External power supply 6510 0004 To connect the SRD to the power source if the SRD is not unit operated together with the pump of the UltiMate 3000 system 9 3 Consumables and Spare Parts Solvent line analytical Blind fittings for the degasser Solvent filter filter holder top and bottom parts set of 6 filters Filter frits in the solvent filter Filter frit stainless steel porosity 10 um set of 10 frits for 6268 0110 RS SD A and M pumps Filter frit titanium porosity 2 um set of 10 frits for 6828 0117 BM pumps and NCS 3x00 Filter frit titanium porosity 10 um set of 10 frits for 6828 0111 AB pumps Bottle cap for solvent reservoirs including caps to close the holes in the bottle cap 4 caps Retaining guide for solvent bottle set of 5 guides Set of caps and retaining guides for solvent bott
7. Avant de commencer utiliser l instrument assurez vous que vous vous tes familiaris s avec le contenu de ce manuel Observez les tiquettes d avertissement sur l appareil et r f rez vous aux sections correspondantes dans ce mode d emploi Veuillez observer les consignes g n rales de s curit suivantes lorsque vous utilisez l instrument ou que vous proc dez des op rations de maintenance e Installez le syst me HPLC dans un laboratoire bien ventil Si la phase mobile contient des solvants volatils ou inflammables emp chez qu ils ne p n trent dans l espace de travail e Afin d viter au maximum les interf rences tous les l ments du syst me analytique doivent tre raccord s la m me ligne secteur m me phase e Lorsque vous soulevez ou l instrument tenez le toujours par le dessous ou par les c t s de l unit Soulever l instrument par la partie avant inf rieure ou par le panneau avant peut endommager la porte e Pour raison de s curit ne soulevez pas l instrument avec des r servoirs e Ne placez aucun objet lourd sur la porte ouverte du panneau avant Ceci pourrait endommager la porte e R glez toujours une limite de pression minimum pour le syst me HPLC Ceci pr vient les dommages r sultant de fuites ou du fonctionnement sec de la pompe e Si la phase mobile contient des solvants volatils ou inflammables vitez les flammes nues et les sources d tincelles proximit e
8. Client Reference Burkhard Seyferth Tested to UL 61010 1 2004 CAN CSA C22 2 61010 1 2004 Certified Product Solvent Rack License Fee Units Model Designation SR 3000 SRD 3100 SRD 3200 SRD 3300 SRD 3400 SRD 3500 SRD 3600 SRD 3700 SRD 3800 Rated Voltage DC 24V Rated Power 15W Protection Class III Special Remarks Replaces Certificate CU72050110 Appendix 1 Licensed Test mark Signatures Date of Issue day mo yr Ei gt d d 01 05 2006 t d o e Dipl Ing M Raap Stephan Schmitt President QA Certification Officer TUV Rheinland of North America Inc 12 Commerce Road Newtown CT 06470 Tel 203 426 0888 Fax 203 426 4009 Page Il Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks Table of Contents e VO D E 1 1 1 How to Use fhis Mandal NEE 1 1 2 Safety Informations ssena naa ee a e a 2 1 2 1 Symbols on the Instrument and in the Manual 2 1 2 2 General Safety Ee 3 1 23 Consignes G n rales de S curit ue 5 1 3 reelle H 1 4 Federal Communications Commission FCC Note ccc ecceseseereeeeees 7 D gt OAL 4 CA ATE IEO A EERE T E E EER 9 2 1 Beeler EE H 2 2 SRD Configurations akeen eelere 9 2 3 Front Panel Elements ss ann nig ce E dirt ttes Aue eee os 11 2 4 Rear Panelni Ds a A ER EE 12 2 5 a Ree E 13 2 6 eelere nn nn 14 2 7 KE 14 3 D taba 1 111 ER ee RER Rene Re Ee 15 3 1 Facility Reguitements EST Mex TT nan a 15 32 Beete TEE 15 3 3 Positioning the SRD in the Ul
9. Panel Tabset or click the corresponding icon on the toolbar E and then connect to the Chromeleon server Chromeleon creates centralized control panels called panel tabsets for all timebases available on the Chromeleon server A panel tabset provides control panels for the individual instruments in a timebase and in addition one or more panels for performing system wide functions for example creating and running sequences For more information about panel tabsets see the Chromeleon Help 2 On the panel tabset for your timebase click the pump page Fig 11 page 31 or NCS page Home Sequence Cortect Heed Separation Pump Sampler Col Comp DAD Commands Flow Pressure Audit Trail nt min 2 477 mm Disconnect Hold StopFlove Wellness Relays Inputs 4 Ret Time Signals Pump Pressure Fig 11 Control panel for the pump here RS pump on a panel tabset Operating Instructions Page 31 UltiMate 3000 Series SRD 3x00 Solvent Racks 3 Verify that the pump or NCS is connected to Chromeleon the LED next to the Connect button is green If it is not click Connect 4 Click More Options and select On or Off under Degasser Mode To turn the degasser on or off from the Commands dialog box 1 In Chromeleon open a control panel for the pump or the pump module of the NCS respectively and connect to the timebase in which the pump is installed For details see Pump and or
10. SRD 3x00 Solvent Racks 2 3 Front Panel Elements POWER 1 VACUUM STATUS Fig 1 Front panel view No Front Panel Description Element 1 LEDs Power The LED is blue when the SRD power is on Vacuum The LED is green if the degasser is working properly The LED is red if the degasser vacuum is insufficient for proper degassing In this case also the Status LED is red Status The LED is green if the degasser is working properly and no leaks have been detected The LED is red if the leak sensor reports a leak The Vacuum and Status LEDs are red if the degasser vacuum is insufficient for proper degassing 2 Standby button Switches the SRD to standby mode The LED of the standby button is red when the SRD is in standby mode To resume operation press the standby button again the LED is not lighted Note To allow the SRD to change the mode press and hold the Standby button for at least 1 second A Important If you switch a pump to which an SRD 3x00 Solvent Rack is connected to the Standby mode the Solvent Rack too will be set to Standby mode A Important Si vous commutez une pompe a laquelle est raccord un d gazeur SRD en mode Veille le d gazeur passera galement en mode Veille Operating Instructions Page 11 UltiMate 3000 Series SRD 3x00 Solvent Racks 2 4 Rear Panel Fig 2 Rear panel view 15 pin D Sub port Connects the SRD to the pump
11. The SRD has been developed as part of the UltiMate 3000 system and is a reliable module for the secure and functional positioning of the solvent reservoirs and for automatic degassing of typical HPLC high performance liquid chromatography solvents The SRD is controlled by the Chromeleon Chromatography Management System from the HPLC pump of the UltiMate 3000 series However the SRD can be used with other HPLC systems if adequate control inputs and outputs are available Being part of the UltiMate 3000 system the SRD and the pump can also be operated with other data systems such as Analyst Applied Biosystems MDS Sciex Compass HyStar Bruker Daltonics or Xcalibur Thermo Scientific To do so installation of the DCMS Dionex Chromatography Mass Spectrometry Link software is required in addition to the installation of the data system Operation in stand alone mode is possible as well Please note that the SRD may be operated only by using Dionex accessories and spare parts page 49 and within its technical specifications page 47 If there is any question regarding appropriate usage contact Dionex before proceeding Dionex cannot be held liable for any damage material or otherwise resulting from inappropriate or improper use of the instrument 1 4 Federal Communications Commission FCC Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part
12. and reformation of gas bubbles in the solvent make sure that the reservoirs are on the same level or higher as the pump Therefore stack the rack onto the pump as shown in Fig 4 page 17 Normal phase eluents usually show only a low concentration of dissolved gases Therefore it is normally not required to use a degasser with these eluents Before connecting the solvent lines make sure that the connectors are free of contaminants Even minute particles can allow air to enter the degasser and thus reduce the degassing effectiveness Page 22 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks e When replacing solvents make sure that the solvents are miscible Mix immiscible solvents with an intermediate solvent for example isopropanol to replace them step by step D Tip Ifthe UltiMate 3000 system includes an UltiMate 3000 series autosampler Dionex recommends degassing the wash liquid on a continuous basis for example by using the degasser of an appropriate SRD The procedure how to prepare and install the wash liquid lines is similar to the connecting the solvent lines For details see the Operating Instructions for the autosampler 4 3 2 Connecting the Solvent Reservoirs 1 Feed the solvent line through the retaining guide which holds the tubing in place in the bottle and then into the open hole in the reservoir cap 2 Verify that an appropriate frit is installed in the filter holder Co
13. ccsssssscasseesddvatasdccssatessaasevsandatandsnaae 35 D Troubl shootingsss sssssssscssssssossssssscssiccossesotssossessssssossossisssovassoecssssssssssos rsi ant enten Sn 37 6 1 RAR 37 6 2 Messages on the Pump Display ss suisse hate 38 6 3 Chromeleon Diagnostics Message eet ee dee 38 6 4 Operating PrOBI MS EE 39 ol CO RSR ER ER En 43 Sc General E AR Tr rie 43 72 Rinsing the Degassing Channels g eegent 44 7 3 Eliminating Leakage in the Solvent Rack 45 8 Technical Information se soeseeseessoesoesoessoesoeseeseoesoesseseoesoossessoesoossessossoossessossoossossoseeoe 47 9 Accessories Consumables and Spare Parts sseessesssooesooessoesssessseessoossoossoosessesssese 49 9 1 ER Re EE 49 9 2 HEES 51 9 3 Consumables and Spare E int nn an ne Re nn ne RE nt 51 10 Pin ASSIPRMENE E 53 10l TS AUD SUD Eege 53 PO RDS ET oi i AEE EE E A E A oo ahs a es 53 Ts E 55 Page ii Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 1 Introduction 1 1 How to Use this Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader However in order to obtain a full understanding of the Solvent Rack Dionex recommends that you review the manual thoroughly before beginning operation Almost all descriptions in the manual apply to all models in the UltiMate 3000 SRD 3x00 Solvent Rack series Therefore the term the SRD is used throughout the manual If s
14. degasser should be as short as possible to avoid reformation of gas bubbles in the solvent Operating Instructions Page 25 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 26 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 5 Operation and Maintenance 5 1 Choosing the Solvents Observe the following precautions Use only water of at least HPLC grade or better LC MS grade 0 2 um filtered If water from water purification systems is used that are not properly maintained polymeric contamination may seriously damage the column rapidly block solvent frits and result in early piston seal wear Use only standard solvents and buffers that are compatible with all parts of the UltiMate 3000 system that may be exposed to solvents For information about the wetted parts in the Solvent Rack see the Technical Information section page 47 For information about the wetted parts in the other UltiMate 3000 system modules refer to the Technical Information section in the operating instructions for the modules Make sure to use special highly pure solvents They are usually labeled accordingly by the vendor Mind the special properties of the solvents such as viscosity boiling point UV absorption UV VIS detector refractive index refractive index detector and dissolved gas degasser Buffer concentration Typically up to 1 mol L lt 0 1 mol L chloride ions pH range 1 through 1
15. page 19 In this case connect an external power supply unit part no 6510 0004 from here to the main power supply page 19 2 DC Input Power Only to be used if the SRD is not controlled by the UltiMate 3000 system pump Page 12 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 2 5 Fluid Connections The front panel door tilts upward to provide easy access to the fluid connections in the SRD The open door locks in its topmost position a Description ATT Fig 3 SRD with open front panel door here SRD 3600 2 Tubing guides for the solvent lines guides for the solvent lines Exhaust air outlet Oh Connect tubing to vent vapors exiting the degassing chambers Leak sensor page 14 Warning D Avertissement A Important A Important For safety reasons you should not lift the SRD with one or more solvent reservoirs being in the tray Pour raison de s curit ne soulevez pas l instrument avec des r servoirs Do not lift the SRD by the front panel door When lifting or moving the SRD always lift by the bottom or sides of the instrument Lifting the SRD by the front panel may damage the front panel door The open front panel door is not designed to carry weight Therefore you should not place any objects on the open front panel door Lorsque vous soulevez ou l instrument tenez le toujours par le dessous ou par les c t s de l unit Souleve
16. serial number of the SRD and follow the steps of the wizard For more information about the test see the Chromeleon Help If the test fails see page 38 for a short description of possible causes along with recommended courses of action Operating Instructions Page 33 UltiMate 3000 Series SRD 3x00 Solvent Racks 5 5 Shutting Down the SRD Observe the following precautions before interrupting the operation or before shipping the SRD e Thoroughly rinse the degasser with alcohol after operation e For longer periods of inactivity and when using saliferous buffers which may result in salt crystallization in the gas separation membrane thereby impairing the degassing performance rinse with de ionized water followed by either methanol or ispropanol e Before returning an SRD to Dionex for repair contact Dionex Service or your local distributor An RMA Return Material Authorization number is required in order to track your instrument Always use the original packaging and observe the packing instructions when shipping the SRD Shipping the SRD in anything other than the original packaging will void the warranty For more information see the warranty statement in the terms of sale When the SRD is connected to the pump of the UltiMate 3000 system and when you are running Chromeleon you can set the pump with the SRD and HPLC system into the standby mode or automate system shutdown For details see the Pump or NCS Manual and Ch
17. with all local regulations Do not allow flammable or toxic solvents to accumulate Follow a regulated approved waste disposal program Never dispose of flammable or toxic solvents through the municipal sewage system e Use only standard solvents HPLC grade and buffers that are compatible with all parts that may be exposed to solvents page 47 e In an UltiMate 3000 system some components are made of PEEK While this polymer has superb chemical resistance to most organic solvents it tends to swell when in contact with trichlormethane CHCI3 dimethyl sulfoxide DMSO or tetrahydrofuran THF In addition it is attacked by concentrated acids such as sulfuric acid and nitric acid or a mixture of hexane ethyl acetate and methanol Swelling or attack by concentrated acids is not a problem with brief flushing procedures e Donot use PEEK tubing that is stressed bent or kinked e Wear goggles when handling fused silica tubing during installation cutting etc e Before interrupting operation for several days or more observe the precautions on page 34 e Use original Dionex spare parts only Substituting non Dionex parts or using non Dionex accessories may impair the performance of the SRD e Do not use the SRD in ways other than those described in these operating instructions Page 4 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 1 2 3 Consignes G n rales de S curit D Veuillez noter
18. 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his expense Operating Instructions Page 7 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 8 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 2 Overview 2 1 Unit Description The SRD is a modern high quality instrument designed for HPLC analysis especially as part of the UltiMate 3000 system The SRD provides a secure location for installation of the solvent reservoirs and when mounted on top of the UltiMate 3000 system saves valuable bench space The SRD includes a reservoir tray solvent reservoirs and appropriate tubing The integrated analytical vacuum degasser continuously degasses solvents through special polymer membranes that are permeable to gas but not to liquids Degassing solvents guarantees reproducibility and reduces pulsation in the connected pump All parts in the flow path are made of Teflon AF PEEK FEP and Tefzel filter frit in the inline filte
19. 3 In rare cases reactions with the stainless steel or titanium have been observed for pH values lt 3 in combination with special solvents and longer application times Therefore you should purge the system thoroughly after these applications and monitor the system behavior Observe the solvent compatibility of the degasser Whenever possible avoid using the following solvents hexafluoroispropanol hydrofluorid containing solvents perfluorinated solvents and freons The UltiMate 3000 series pumps are shipped with reversed phase piston seals RP Keep in mind that using chloroform trichlorobenzene methylene chloride tetrahydrofuran or toluene as solvents chemically damages the UHMW PE seals Chemical reactions may also occur when using tetrachloromethan diethyl ether ethyl ether di isopropyl ether ketones methylbenzene methycyclohexane and monochlorobenzene If you use these solvents contact your Dionex sales representative In an UltiMate 3000 system some components are made of PEEK While this polymer has superb chemical resistance to most organic solvents it tends to swell when in contact with trichlormethane CHCI3 dimethyl sulfoxide DMSO or tetrahydrofuran THF In addition it is attacked by concentrated acids such as sulfuric acid and nitric acid or a Operating Instructions Page 27 UltiMate 3000 Series SRD 3x00 Solvent Racks mixture of hexane ethyl acetate and methanol Swelling or attack by c
20. 44 Fluid Connections 13 RIMES NOR cosets akan Ser PUMP EE 25 S Solvent Reservoirs cecceseeseeeteeseeesseeeeees 23 See ar ane PARA tre Ee 2 I Safety Precautions cccccccccscsesecssseseseescecseneeseees 3 Service E e Nee 1 De Che owe cere 44 ntended USE sise dents on Gopal EE 43 Leak Sensor n s SATA a felled 45 Operating Instructions Page 55 UltiMate 3000 Series SRD 3x00 Solvent Racks Shut DOWN Ee wie at 34 U ee ENEE 15 Solvent Unit Description Overview 9 Compatibility ccccccscscsssesssssssssessssssseeseeee 27 Uer 15 Degasser Compatibility 27 e 27 V Solvent Rack Leak 33 NACRE retenu etes on 33 Solvent Reservoirs 22 Vacuum Degasser Solvent Compatibility 27 CONN CT een nent 22 Vacuum Monitoring 33 G n ral Notes nu dents ints 22 Spare Parts issues init nt exec 51 W Specification EE 47 SRD Display SEENEN Re 11 Wellness We hosel Mers foes 14 SRD Drainage 21 Standalone Operation c cecseesceeseeeeeteetees 32 Eelsten aa eege 2 T Troubleshooting 37 Diagnostics Messages 38 Display Messages ss sesseeseseeeseseesereesseeeessee 38 Operating Problem 39 Page 56 Operating Instructions
21. Action Check the connection to the pump or NCS of the UltiMate 3000 system or the connection of the external power supply unit page 18 Verify that the degasser is turned on If the SRD is operated from the pump or NCS of the UltiMate 3000 system see page 30 If the SRD is operated in standalone mode see page 32 Press the Standby key on the front panel Contact Dionex Service Check the USB cable and connection to the computer Check the USB port on the computer Check the connection to the pump or NCS gt page 19 Clean or replace the column Flush the system until equilibration Use fresh solvent and check the eluent filter frits In aqueous solvents growth of microorganisms is possible Make sure that the temperature and the humidity are constant Operating Instructions Page 39 UltiMate 3000 Series SRD 3x00 Solvent Racks High baseline drift Cont d Poor reproducibility The detector has not yet reached the operating temperature The solvent is delivered in circles The flow cell is dirty The lamp is too old The autosampler draws air from the vial Poor degassing There are air bubbles in the syringe There are air bubbles in the flow path The autosampler the injection valve or the syringe valve is not tight Carry over occurs in the system The capillary connections are not installed properly or they are not tight There are d
22. CT pe RS and SD pumps Stainless steel porosity 10 um 6268 0110 BM pumps and NCS 3x00RS Titanium porosity 2 um 6268 0117 AB pumps Titanium porosity 10 um 6268 0111 3 Slide the filter holder with filter frit onto the end of the solvent line 4 Ifrequired Cut the tubing straight if necessary The end of the solvent line should be cut straight and not deformed Use only the original Dionex solvent lines 5 Place the entire assembly in the solvent reservoir 6 Tighten the reservoir cap hand tight E Retaining guide K C Filter holder with ef filter frit Fig 7 Connecting the solvent lines to the reservoirs When replacing a solvent line remove the frit first then the solvent line and then the retaining guide 7 Connect the lines to the appropriate connection ports on the degassing module Route the solvent lines through the opening between the housing and the front panel door Operating Instructions Page 23 UltiMate 3000 Series SRD 3x00 Solvent Racks Fig 8 Opening for the solvent lines 8 Remove the blind fitting from the degasser inlet if necessary and connect the solvent line with the associated line label Degasser inlets je je e e je le solvent line connection A BC A BE o Degasser outlets i i to pump Tubing guides Fig 9 Example for degasser connections here SRD 3600 D Tip The degasser ports in the upper row are combined in pairs with the related ports in t
23. DIONEX UltiMate 3000 Series SRD 3x00 Solvent Racks Operating Instructions Revision 1 4 Date January 2010 2010 Dionex Doc No 4820 3550 UltiMate 3000 Series SRD 3x00 Solvent Racks Operating Instructions DIONEX CE Declaration of Conformity Product Solvent Rack Types SR 3000 SRD 3200 SRD 3400 and SRD 3600 Dionex GmbH herewith declares conformity of the above products with the respective requirements of the following regulations e Low Voltage Directive 2006 95 EC e EMC Directive 2004 108 EC The electrical safety of the products was evaluated based on the following standard e EN 61010 1 2002 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General Requirements The Electromagnetic Compatibility EMC of the products was evaluated based on the following standards e DIN EN 61326 2006 Electrical equipment for measurement control and laboratory use EMC Requirements This declaration is issued for the manufacturer Dionex Softron GmbH DornierstraBe 4 D 82110 Germering by the President Dr Peter Jochum January 15 2010 Operating Instructions Page UltiMate 3000 Series SRD 3x00 Solvent Racks Certificate TUV License Holder Manufacturing Plant Dionex Softron GmbH Dionex Softron GmbH Dornierstr 4 Dornierstr 4 82110 Germering 82110 Germering Germany Germany Test report no USA RC 30580091 003
24. NCS Manual and the Chromeleon Help 2 On the Control menu select Command 3 Depending on the pump type click the plus sign next to PumpModule or Pump to display the items underneath 4 Set Degasser to On or Off to turn on or off the degasser Commands UltiMate_RLSC EE Modelo A Retention Time min D I ModelVariant J D I HeadType Degasser Off On RearSealWashPump RearSealwashStatus On Leak SolventRackLeak LeakSensorMode OverideRearSealDry Degasser 0 Degasservacuum Ready Alarm v gt A Fig 12 Commands dialog box To turn the degasser on or off automatically It is also possible to include the command for turning the degasser on or off in a program file PGM for automated operation of the pump For details about program creation see the Pump Manual and Chromeleon Help 5 3 2 Turning the Degasser On or Off Stand alone Operation If the SRD is directly connected to the power source by using the external power supply unit page 19 the degasser reaches the appropriate operating vacuum after a short time When the front panel LEDs are blue green green the degasser is ready for operation Page 32 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 5 4 Special Chromeleon Functions This section provides a short overview of some special functions that Chromeleon supports for the SRD These functions are available in the Commands dialog
25. Operating Instructions cceeceseeeseeeeereeeteeees 1 Configurations nine 9 i Operating Problems 39 Connection Operations saciid ncn acai 27 15 pin D Sub nanas ins ks 19 After Power Failure 33 DC Input EE 19 Control Elements 11 WIS 225 ee ea ns 13 P 19 Desass r rnern an i EA 29 UMP egiri nee from UltiMate 3000 System nu 30 Solvent Reservoirs ccccesceeseeeteeseeeseeeeeees 23 F Safety Precautions cccceccceeseeeseereeeeeeerees 3 Consumables 51 s Control El t 11 ETH Eeer R der ee ie 30 STEE EE Solvent Selection 0 cceccsseeseesceesteeneeeteeees 27 D SRD Display 11 Standalone eege eeh 32 Reg ll E 19 with Chromeleon ccccecceeseeeeeeeeeteeeteeees 30 Degasser Solvent Compatibility 27 Degasser Operation Notes 29 P es ls Ri EE KE A Pin Assignment 53 Pi SAANS Res Piston Seal Solvent Compatibility 0 27 IR E 33 l 5 i Power Failure sisi au he eek 33 Diagnostics Messages 38 Power Supply Gvtermal 19 Diagnostics Tests ne Ne ner 33 Por 21 Drain System cccceccceesceeeeseceeeceteceseenseeseensees 21 EE ER Drainage R KLORE 21 SYSTEM serrer rente a E rent den dev 21 Rear Pa nel ssstahunnaltsandt 12 19 Rear Panel Connectors sss sessssesseeseeseeserseeseeee 12 F Rinse Degassing Channels
26. Si une fuite se produit arr tez imm diatement l instrument et rem diez au probl me e Remplacez les cordons d alimentation lectrique et les c bles de communication d fectueux e De nombreux solvants organiques et solutions salines sont toxiques Informez vous des propri t s toxicologiques de toutes les phases mobiles que vous utilisez e Les propri t s toxicologiques de nombreux chantillons peuvent tre mal connues Au moindre doute concernant un chantillon traitez le comme s il contenait une substance potentiellement dangereuse Operating Instructions Page 5 UltiMate 3000 Series SRD 3x00 Solvent Racks e Portez des lunettes de protection lorsque vous manipulez des phases mobiles ou que vous utilisez l instrument Une installation permettant de se laver les yeux ainsi qu un lavabo doivent se trouver proximit du syst me Si une phase mobile quelle qu elle soit entre en contact avec vos yeux ou votre peau rincez abondamment la zone affect e l eau puis e D barrassez vous de tous les d chets de phase mobile de mani re cologique conform ment la r glementation en vigueur au niveau local Emp chez imp rativement l accumulation de solvants inflammables et ou toxiques Suivez un programme d limination des d chets r glement et approuv Ne jetez jamais de solvants inflammables et ou toxiques dans le syst me municipal d vacuation des eaux us es e Utilisez uniquement des solvants q
27. ame phase e The front panel tilts upward When lifting or moving the SRD always lift by the bottom or sides of the instrument This is to avoid damage to the instrument e For safety reasons you should not lift the SRD with one or more solvent reservoirs being in the tray e The open front panel door is not designed to carry weight Therefore you should not place any objects on the open front panel door e Always set a lower pressure limit for the HPLC pump This prevents damage resulting from leakage or from running the pump dry e Ifthe mobile phase includes volatile or flammable solvents avoid open flames and sparks e Ifa leak occurs turn off the SRD and remedy the situation immediately e Replace faulty power cords and communication cables e Many organic solvents and buffers are toxic Know the toxicological properties of all mobile phases that you are using e The toxicological properties of many samples may not be well known If you have any doubt about a sample treat it as if it contains a potentially harmful substance e Wear goggles when handling mobile phases or operating the instrument An eyewash facility and a sink should be close to the unit If any mobile phase splashes on the eyes or skin wash the affected area and seek medical attention Operating Instructions Page 3 UltiMate 3000 Series SRD 3x00 Solvent Racks e Dispose of waste mobile phase in an environmentally safe manner that is consistent
28. ance of the instrument A Important Indicates that failure to take note of the accompanying information could cause wrong results or may result in damage to the instrument A Important Indique que ne pas tenir compte de l information jointe peut conduire de faux r sultat ou endommager l instrument D Warning Indicates that failure to take note of the accompanying information may result in personal injury D Avertissement Indique que ne pas tenir compte de l information jointe peut entra ner des blessures corporelles Page 2 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 1 2 2 General Safety Precautions When working with analytical instrumentation you should know the potential hazards of using chemical solvents D Tips Before initial operation of the SRD make sure that you are familiar with the contents of this manual Observe any warning labels on the device and see the related sections in these Operating Instructions To avoid the possibility of personal injury or damage to the instrument observe the following general safety precautions when operating the instrument or carrying out any maintenance work e Install the HPLC system in a well ventilated laboratory If the mobile phase includes volatile or flammable solvents do not allow them to enter the workspace e For minimum interference effects all components of the analytical system should be connected to the same mains output s
29. box for the pump or on the control panel for the pump For more information see the Chromeleon Help 5 4 1 Leak and Vacuum Monitoring The following SRD related parameters appear in the Commands dialog box under PumpModule or Pump depending on the pump type Degasser Vacuum Reports whether the degasser in the SRD has reached the operating vacuum SolventRackLeak Reports whether the leak sensor in the SRD detected a leak If the SRD is controlled by Chromeleon together with the pump as part of the HPLC system the program file PGM File can include a command that automatically restarts operation as desired after a power failure For details see the Chromeleon Help 5 4 2 SRD Diagnostics Chromeleon 6 80 supports Diagnostics functions for the pumps of the UltiMate 3000 system also allowing you to check the performance of the solvent rack degasser For details see the Pump or NCS Manual and Chromeleon Help You can run the test for an SRD for which the last four digits of the serial number end in 0701 or higher or for which the serial number starts with is 8 or 9 The serial number is provided on the type label on the rear of the SRD 1 On the Control menu select Diagnostics The Control menu is visible only when a control panel is open 2 The Diagnostics dialog box lists all tests that are available for the devices in the current timebase Select the Degasser Vacuum Test 3 A wizard guides you through the test Enter the
30. button on the front of the solvent rack page 11 Press and hold the button for one second to allow the SRD to change the mode Observe the following e Turning off the pump to which the SRD is connected also turns off the SRD The same applies to Standby mode e Dionex recommends always leaving the degasser on 5 3 1 1 Turning the Degasser on and off from the Pump Display 1 On the front panel display of the pump show the function keys and select Menu For details see the Pump Manual and or NCS Manual 2 On the Main menu select Preferences and then select Degasser 3 Select On or Off to turn on or off the degasser Page 30 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 5 3 1 2 Turning the Degasser on and off in Chromeleon If the pump is operated with Chromeleon select one of the following alternatives to turn the degasser on and off e Directly from the Panel Tabset page 31 e Directly from the Commands dialog box for the pump page 32 e Automatically with a program file PGM for the pump page 32 If the Solvent Rack is connected to an UltiMate 3000 SD RS or BM pump or to an NCS 3x00RS verify in the Properties dialog for the device that Degasser Control is set to External with SD RS and BM pumps on the Devices page with the NCS on the Pumps page To turn the degasser on or off from the Panel Tabset 1 In Chromeleon click the View menu and Default
31. d with Chromeleon the associated message appears on the pump display and also in the Chromeleon Audit Trail page 37 Page 14 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 3 Installation 3 1 Facility Requirements Make sure that the installation site meets the power and environmental specifications listed in the Technical Information section page 47 Install the SRD in the laboratory on a stable surface that is free of vibrations Typically the SRD is placed on top of the pump Make sure that the surface is resistant to solvents Avoid locations with extreme changes in temperature Avoid locations with extreme changes in temperature such as direct sunlight or drafts and high humidity Allow sufficient clearance behind and to the sides of the SRD for power connections and ventilation 3 2 Unpacking All electrical and mechanical components of the SRD are carefully tested before the instrument is shipped from the factory After unpacking inspect the SRD for any signs of mechanical damage that may have occurred during transit D Tips Immediately report any shipping damage to both the incoming carrier and Dionex Shipping insurance will compensate for the damage only if reported immediately Keep the original shipping container and packing material They will provide excellent protection for the SRD in case of future transit Shipping the unit in any other packaging automatically voi
32. ds the product warranty Place the shipping container on the floor Remove the accessories kit Grasp the SRD by the sides Slowly and carefully pull the SRD out of the shipping container and place it on a stable surface A Important To prevent the SRD from falling grasp the SRD by the sides and then lift the SRD together with the foam spacers out of the shipping container Do not lift the SRD by the foam spacers or the front panel Operating Instructions Page 15 UltiMate 3000 Series SRD 3x00 Solvent Racks A Important Afin d emp cher l instrument de tomber saisissez la par les c t s Ne soulevez l instrument l aide du mat riau d emballage ou par la porte du panneau avant 3 Remove the foam spacers and then remove the polythene packaging 4 Before connecting the SRD to the power source wait approximately 4 hours to allow the instrument to come to room temperature and to allow any condensation that might have occurred during shipping to evaporate After 4 hours check the SRD if condensation still exists allow the SRD to continue to warm up without connecting it to the power source until the condensation is completely gone Page 16 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 3 3 Positioning the SRD in the UltiMate 3000 System If the SRD is part of an UltiMate 3000 system for example for analytical HPLC applications Dionex recommends that you stack the individual modules
33. e Temperature 10 C to 35 C 50 to 95 F Air humidity 80 relative humidity non condensing Overvoltage category II Dimensions H x W x D 10 x 42 x 51 cm Technical information April 2009 All technical specifications are subject to change without notice Operating Instructions Page 47 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 48 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 9 Accessories Consumables and Spare Parts Accessories consumables and spare parts for the SRD are always maintained at the latest technical standard Therefore part numbers are subject to alteration However updated parts will always be compatible with the parts they replace 9 1 Standard Accessories The following accessories are shipped with the instrument the list is subject to change without notice The part number always refers to the packing unit Unless otherwise stated the packing unit is 1 unit For more information contact the Dionex sales organization Description SRD 3600 accessories including Cable connecting the SRD and the UltiMate 3000 system pump or NCS Cap to close the holes in the solvent bottle caps Bottle cap for solvent reservoirs including caps to close the holes in the bottle cap Solvent filter including Filter holder top and bottom parts and filter frit stainless steel 10 Retaining guide solvent bottle 1 liter solvent reservoir Solve
34. ead volumes in the capillary connections Probable Cause Remedial Action Allow the full detector warm up time Direct the solvent to waste Clean the flow cell Replace the flow cell if necessary Detector Manual Replace the detector lamp gt Detector Manual There is not enough amount of sample in the vial the needle height setting is incorrect Autosampler Manual or there are too many replicates Inspect the capillary and solvent tube connections for leakage tighten loose fitting connections Reduce the flow rate Flush the syringe Autosampler Manual Perform a wash cycle Autosampler Manual Non degassed wash liquid is used Degas the wash liquid Autosampler Manual Inspect the autosampler and the connections on the injection valve and syringe valve Tighten leaking connections It might be necessary to replace the injection valve or syringe valve In this case contact Dionex Service Flush the needle using an appropriate solvent Check and tighten the capillary connections Exchange the needle port if necessary Autosampler Manual Exchange the needle if necessary Autosampler Manual Replace the fittings Make sure that the capillaries are installed correctly Page 40 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks Poor reproducibility Cont d Poor degassing RS SD and BM pumps The piston seals a
35. erformance of the SRD degasser page 33 Operating Instructions Page 35 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 36 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 6 Troubleshooting 6 1 Overview The following features help you to identify and eliminate the source for problems that may occur during the operation of the SRD or UltiMate 3000 system Status Indicators The status indicators on the front panel display provide a quick visual check of the operational status of the SRD They indicate whether the SRD is turned on and operating properly page 11 Messages If the SRD is operated from a pump of the UltiMate 3000 system and if a fault or error is detected during the operation of the SRD a message appears on the front panel display of the pump Check the Messages on the Pump Display section for recommended courses of action page 38 If the pump is operated with Chromeleon a message is also displayed in the Chromeleon Audit Trail D Tip For information about operating problems that might occur during the operation of an UltiMate 3000 system see Operating Problems page 39 Diagnostics Test When the SRD is operated with the pump of an UltiMate 3000 system and when the pump is operated by Chromeleon you can check the performance of the SRD degasser from Chromeleon page 33 If the test fails check the Chromeleon Diagnostics Message section for a
36. he lower row Before connecting the solvent lines make sure that the connectors are free of contaminants Even minute particles can allow air to enter the degasser and thus reduce the degassing effectiveness 9 Connect the solvent lines from the pump to the degasser page 25 Page 24 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 4 3 3 Connecting the Solvent Lines from the Pump to the Degasser With all UltiMate 3000 system pumps the solvent supply lines are connected to the pump at the factory 1 Ifnecessary route the solvent lines through the opening between the front panel door and top cover Fig 10 Regen gg et ei The solvent lines are routed through this opening Pump housing Pump door i Fig 10 Preconnected solvent lines here on a DGP 3600 2 Tubing guides in the SRD facilitate the connection of the solvent lines Fig 9 page 24 Place the solvent line in a tubing guide remove the blind fitting from the associated degasser outlet if necessary Fig 9 and connect the solvent line with the associated line label 3 Locate the solvent reservoirs as close as possible to the pump To avoid formation of gas bubbles in the reservoirs and reformation of gas bubbles in the solvent make sure that the reservoirs are on the same level or higher as the pump Therefore stack the SRD onto the pump as shown in Fig 4 page 17 The tubing connecting the pump to the
37. hten loose fitting connections Reduce the flow rate Operating Instructions Page 41 UltiMate 3000 Series SRD 3x00 Solvent Racks Probable Cause Remedial Action Rinse the degassing channels page 44 Reproducible ghost peaks in the chromatogram Degasser noise The degassing channels are contaminated The solvents are degraded or dirty or their purity is insufficient Contamination occurs somewhere in the system The vacuum pump of the degasser is running at high speed Use fresh and appropriate solvents Flush the system using an appropriate solvent Fill and degas all channels even if they are not used for the application Page 42 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 7 Service 7 1 General Notes The following sections describe all service and repair procedures for the SRD that the user may perform All other maintenance and service procedures must be performed by Dionex personnel Observe the following precautions e Keep in mind that the fluid components of the SRD may be filled with toxic solvents Before starting maintenance rinse toxic solvents from the instrument and put on protective clothing e Use original Dionex spare parts only Substituting non Dionex parts or using non Dionex accessories may impair the performance of the instrument thereby voiding the product warranty e Before returning an SRD to Dionex for repair
38. les including 6030 9101 5 caps to close the holes in the solvent bottle caps and 10 retaining guides for solvent bottle Operating Instructions Page 51 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 52 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 10 Pin Assignment 10 1 15 pin D Sub Port leet Tea Je Oo T oe O pe O EE a a Co pee O EE Ka a a DE PL DC a a E TC LC ofe f i pe E a a Fig 14 15 pin D Sub port male 10 2 DC Input The DC input is used to connect the SRD to the main power supply This is required only if the SRD is not operated from an UltiMate 3000 system pump e 24 Volt DC tee Fig 15 DC input Operating Instructions Page 53 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 54 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 11 Index 15 pin Dees eege 19 L is Leak n E RE A E RR 33 ACCESSOT reen e r a 49 51 Leak Monitoring 33 EE EE 14 45 C M Chromeleon Diagnostics ssrsiranininnsuninienanes 33 Maintenance ooo eee eeseeeseescseeeescneeseeseneeseeeens 35 Error Messages c ccsseceseeseeseeeeeeeeenreeerens 37 Maintenance Intervals 35 Leak Monitoring 0 0 0 cceceeseeseereeeeeereeeeees 33 Messages iii i ico Me ne ogee ioe 38 Operati Ot eerste ge r wees 30 Special FUNCTIONS c ccccccscsesscssseseeeeteeeseees 33 O Macon MONO N rar ten
39. mp display to remove the message Allow the sensor to adjust to the ambient temperature for a few minutes If no errors are reported operation can be resumed D Tip Ifthe sensor is not dry the Status LED remains red Operating Instructions Page 45 UltiMate 3000 Series SRD 3x00 Solvent Racks Page 46 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 8 Technical Information Bottle capacity Eight 1 L reservoirs or four 2 5 L reservoirs or two 5 L reservoirs or five 1 L reservoirs and one 5 L reservoir or three 2 5 L reservoirs and two 1 L reservoirs or three 1 L reservoirs and two 2 5 L reservoirs or sixteen 0 5 L reservoirs Degassing channels SRD 3600 6 vacuum channels analytical SRD 3400 4 vacuum channels analytical SRD 3200 2 vacuum channels analytical Channel volume 670 uL Max flow rate per channel 12 mL min Control Controlled by the UltiMate 3000 system pump with Chromeleon or standalone operation Communication 15 pin D Sub connector through the pump of the UltiMate 3000 system Power supply 15 pin D Sub connector through the pump of the UltiMate 3000 system or external power supply unit optional User input display 3 LEDs Power Vacuum and Status Standby button Wetted parts Teflon AF PEEK FEP and Tefzel Inline filter frit SST or Titanium Safety features Leak monitoring vacuum monitoring Environmental conditions Range of use Indoor us
40. normally used for your application Use fresh HPLC grade water Rinse the degassing channels with fresh HPLC grade water until the pH value is neutral Rinse the degassing channels for two hours with fresh acetonitrile at the flow rate normally used for your application Prepare fresh solvents for your application using new bottles and connect them to the degassing channels Install new filter frits on the solvent lines Uninstall the backpressure capillary and reconnect the system as required by your application Equilibrate the system Page 44 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 7 3 Eliminating Leakage in the Solvent Rack The leak sensor is installed inside the SRD and reports a leak when liquid collects in the drip tray under the fluid connections Locate the source for the leak eliminate the cause and dry the leak sensor l Inspect the degasser inlets and degasser outlets and the connected solvent lines for signs of leaks Tighten or replace leaking connections Dry the components With a cloth or tissue absorb all liquid that has collected in the tray Make sure that you do not bend or damage the sensor Fig 13 Drying the leak sensor If the SRD is operated from the pump of the UltiMate 3000 system the message remains on the pump display for information purposes even after the source for the leak has been eliminated Select Clear on the navigation bar on the pu
41. nt line analytical Tubing labels white A B C D Assortment box Quantit Part No y in the kit 6000 1006 1 0 Included in 6000 0047 E sd a Included in 6268 0115 Included in 6268 0110 Included in 6000 0042 2270 0012 6030 2548 mao 6000 0043 Operating Instructions Page 49 UltiMate 3000 Series SRD 3x00 Solvent Racks Description SRD 3400 accessories including Cable connecting the SRD and the UltiMate 3000 system pump or NCS Cap to close the holes in the solvent bottle caps Bottle cap for solvent reservoirs including caps to close the holes in the bottle cap Solvent filter including Filter holder top and bottom parts and filter frit stainless steel 1011 Retaining guide solvent bottle 1 liter solvent reservoir Solvent line analytical Tubing labels white A B C D Assortment box Description SRD 3200 accessories including Cable connecting the SRD and the UltiMate 3000 system pump or NCS Cap to close the holes in the solvent bottle caps Bottle cap for solvent reservoirs including caps to close the holes in the bottle cap Solvent filter including Filter holder top and bottom parts and filter frit stainless steel 1011 Retaining guide solvent bottle 1 liter solvent reservoir Solvent line analytical Tubing labels white A B C D Assortment box Quantit Part No y in the kit 6000 1006 1 20 Included in 6000
42. ome detail applies to only one model the model is identified by name Note The device configuration may vary therefore not all descriptions necessarily apply to your particular instrument The software descriptions in this manual refer to Chromeleon 6 80 Service Release 8 The information contained in this manual is subject to change without notice and should not be construed as a commitment by Dionex Dionex assumes no responsibility for any errors that may appear in this document This document is believed to be complete and accurate at the time of publication In no event shall Dionex be liable for incidental or consequential damages in connection with or arising from the use of this document The information contained in this document is subject to change without notice CHROMELEON and UltiMate Il are registered trademarks of Dionex All other trade or company names mentioned are subject to the copyright and the property and trademark rights of the respective companies All rights reserved including those for photomechanical reproduction and storage on electronic media No part of this publication may be copied or distributed transmitted transcribed stored in a retrieval system or transmitted into any human or computer language in any form or by any means electronic mechanical magnetic manual or otherwise or disclosed to third parties without the express written permission of Dionex Operating Instructions Page 1
43. oncentrated acids is not a problem with brief flushing procedures e Before switching from buffer to organic solution rinse the system thoroughly with deionized water e When switching to another solvent ensure that the new solvent is miscible with the one contained in the system Otherwise for example the pump can be damaged by flocculation e After operation rinse out buffers and solutions that form peroxides A Important Do not recycle the solvent or use methanol from aluminum reservoirs This may impair the performance of the seals A Important Dionex d conseille de recycler les solvants Ceci peut nuire aux performances des joints Page 28 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 5 2 General Notes for Degasser Operation The degassers run quietly Even if the vacuum pump is running the operating noise is very low With higher load the operating noise may slightly increase However this does not impair the degassing performance In addition observe the following to ensure optimum degassing performance Dionex recommends filling a channels with eluent and degassing all channels even if they are not used for the application This will reduce the speed of the vacuum pump and reduce the degasser noise To avoid contamination of the degasser you should prepare fresh solvents clean the solvent lines and rinse the degassing channels at regular intervals page 44 Dionex ad
44. r stainless steel or titantum to provide optimum resistance to the most commonly used HPLC solvents and buffer solutions 2 2 SRD Configurations The SRD is available in the following configurations SRD 3600 SRD with analytical 6 channel vacuum degasser 5035 9230 typically for use with the following pumps one DGP 3600 two HPG 3200 pumps in a two stack system one HPG 3200 and one HPG 3400 in a two stack system one HPG 3400 if you want to degas the solvents and the wash liquid of the UltiMate 3000 autosampler SRD 3400 SRD with analytical 4 channel vacuum degasser 5035 9245 typically for use with the following pumps one HPG 3400 two HPG 3200 pumps in a two stack system one HPG 3200 or ISO 3100 if you want to degas the solvents and the wash liquid of an UltiMate 3000 series autosampler one NCS 3500RS SRD 3200 SRD with analytical 2 channel vacuum degasser 5035 9250 typically for use with the following pumps one HPG 3200 one ISO 3100 Operating Instructions Page 9 UltiMate 3000 Series SRD 3x00 Solvent Racks In addition a solvent rack without degasser channels is available SR 3000 part no 5035 9200 The SR 3000 is intended only for use as a solvent organizer for example together with an LPG 3400 pump For this pump type the degasser is incorporated in the pump D Tip An SRD with an analytical degasser cannot be used with a semipreparative pump Page 10 Operating Instructions UltiMate 3000 Series
45. r l instrument par la partie avant inf rieure ou par le panneau avant peut endommager la porte Ne lacez aucun objet sur la porte ouverte du panneau avant Ceci peut endommager la porte Operating Instructions Page 13 UltiMate 3000 Series SRD 3x00 Solvent Racks 2 6 Leak Sensor A leak sensor is installed inside the SRD If liquid collects in the drip tray under the fluid connections the leak sensor reports a leak and the Status LED on the front panel door changes to red If the SRD is operated from a pump of the UltiMate 3000 system a message Solvent Rack leak detected appears on the front panel display of the pump When the pump is operated by Chromeleon and if the leak sensor is exposed to liquids for at least 10 minutes the related message is displayed also in the Chromeleon Audit Trail When the leak sensor reports a leak eliminate the cause for the leakage and dry the leak sensor page 45 2 7 Wellness System Wellness monitors the health of the SRD Therefore the SRD supports several performance and reliability features that can help you detect small problems before they turn into big ones e Leak detectionStatus LED on the front panel door e Degasser vacuum level monitoring Status and Vacuum LEDs on the front panel door When an error is detected the Status LED or the Status and Vacuum LEDs on the front panel door change to red If the SRD is connected to an UltiMate 3000 system pump that is operate
46. re not tight There are air bubbles in the pump heads and or flow meter After changing solvents the previous solvent has not been flushed out completely RS SD and BM pumps Pump pulsation The sample is unstable and decomposes The gradient is irreproducible The environmental conditions are unstable Contamination occurs somewhere in the system There is a leak in the capillaries or solvent lines The flow rate is too high Probable Cause Remedial Action Replace the seals Pump Manual Purge the pump check general function Pump Manual NCS Manual Purge the pump NCS Manual Use degassed solvents Check the filter frits in the solvent lines for contamination and replace as necessary Use new sample or change the conditions RS SD and BM pumps Change the gradient Check the pump function and degassing Check the filter frits in the solvent lines for contamination and replace as necessary NCS 3x00RS The pressure transducer offset may not be correct Check the offset and calibrate the pressure transducers if required NCS Manual Make sure that the temperature and the humidity are constant Use a column compartment if necessary Avoid draft Flush the system using an appropriate solvent Check the filter frits in the solvent lines for contamination and replace as necessary Inspect the capillary and solvent tube connections for leakage tig
47. related to the pump see the Pump and or NCS Manual 6 3 Chromeleon Diagnostics Message When the degasser in the SRD fails the Degasser Vacuum Test the degassing module most probably did not reach the appropriate operating vacuum Check the tubing of the vacuum system and the degassing module and repeat the test If the test fails again the degassing module may be defective and should be replaced Page 38 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 6 4 Operating Problems The following table provides information about common operating problems that might occur with an UltiMate 3000 system and lists probable causes as well as remedial actions For more information also see the manuals for the other modules of an UltiMate 3000 system No function The SRD does not work correctly when the pump is controlled by Chromeleon High baseline drift The instrument is not connected to the mains The degasser is turned off The instrument is in standby mode An error occurred in the electronic system There is no connection between the SRD and the Chromeleon computer by using the pump or NCS The USB port on the computer is not ready for operation The degasser is not connected correctly to the pump or NCS The column is contaminated The system is not sufficiently equilibrated The solvent is degraded The environmental conditions are unstable Probable Cause Remedial
48. romeleon Help Page 34 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 5 6 Routine and Preventive Maintenance The SRD is made of high quality components and materials to minimize maintenance requirements All surfaces are resistant to weak acids alkali and organic solvents Nevertheless immediately wipe up all liquids spilled onto the SRD surface by using lint free cloth or paper If surfaces are exposed for longer periods these liquids can cause damage Perform the maintenance procedures listed in the table at regular intervals to ensure optimum performance and maximum uptime of the SRD The exact maintenance schedule for the SRD will depend on a number of factors Regularly To avoid contamination of the degasser e Prepare fresh solvents e Clean the solvent lines e Rinse the degassing channels section 7 2 Check the fluid lines for any signs of leakage Check the fluid connections for any signs of salt deposits Check the drain tube connected to the drain port on the bottom right of the SRD Verify that the tubing is unclogged and is routed below the drain port Check the volume of the liquid in the waste container and empty as needed Annually Have a Dionex service representative check the detector once a year to prevent contamination and excessive wear D Tip Ifthe SRD is operated with the pump of an UltiMate 3000 system and if the pump is operated by Chromeleon you can check the p
49. short description of possible causes along with recommended courses of action page 38 If you are unable to eliminate a problem following the instructions given here contact Dionex Service Operating Instructions Page 37 UltiMate 3000 Series SRD 3x00 Solvent Racks 6 2 Messages on the Pump Display Each time a fault or error occurs during the operation of the SRD the Status LED or the Status and Vacuum LEDs on the front panel door change to red If the SRD is operated from the pump of the UltiMate 3000 system one or more messages appear on the pump display When the pump is operated with Chromeleon the message also appears in the Chromeleon Audit Trail The table lists SRD related messages along with recommended remedial actions In addition to the messages in the table other messages may appear In this case note the exact wording of the message and contact Dionex Service if you are unable to eliminate the problem Degasser malfunction The vacuum level monitoring function of the degasser in the SRD was activated Turn the SRD off and on again by pressing the standby button on the front panel If the message appears again contact Dionex Service Solvent Rack leak detected The leak sensor in the SRD has reported a leak There is a leak in the system or a fluid connection is loose Locate the source for the leak eliminate the cause and dry the leak sensor page 45 D Tip For information about the messages
50. tiMate 3000 System 17 3 4 Connecting the RID srt anh cee Re nn coast au ok 19 3 4 1 Connecting the SRD to an UltiMate 3000 System Pump 19 3 4 2 Connecting the SRD to the Main Power Supply Optional eee 19 4 Preparation for Operation Startup cscccscscsscssscsssscssssssscssssssssesssssssssccesees 21 4 1 COVERY TSW a nn th fe cages thea et nt tte A Pie en st ee 21 4 2 Connecting RE RK bises nr stress Gr hs Msn E 21 4 3 Solvent RESERV OIF rentre datant Are bang Ase REA 22 43 1 G netalINOteS EE masthead n SEE ih 22 43 2 Connecting the Solvent Reservoirs eut eesnislinientereenteteteesse 23 4 3 3 Connecting the Solvent Lines from the Pump to the Degasser 0 0000000000 25 5 Operation and Maintenance reese eebe deeg 27 5 1 Choosing TEE 27 52 General Notes for Degasser Operaton 29 Operating Instructions Page i UltiMate 3000 Series SRD 3x00 Solvent Racks 5 3 Op rational EE 30 5 3 1 Turning the Degasser on or off from the UltiMate 3000 System Pump 30 5 3 1 1 Turning the Degasser on and off from the Pump Display 30 5 3 1 2 Turning the Degasser on and off in Chromeleon ceeecceesseeeteeees 31 5 3 2 Turning the Degasser On or Off Stand alone Operatnon cece 32 5 4 Sp cial Chromeleon e 33 5 4 1 Leak and Vacuum Monitor ssssrstesnetisnaesnttetehianeitaisces 33 5427 RE 33 5 5 Shutting Down th SRD einer iier iie a a ies Mest 34 5 6 Routine and Preventive Maintenance 3 c s is 20
51. ualit HPLC et des solutions salines compatibles avec les mat riaux expos s phase mobiles page 47 e Dans un syst me UltiMate 3000 certaines composantes sont en PEEK Bien que ce polym re pr sente une excellente r sistance chimique la plupart des solvants organiques il a tendance gonfler lorsqu il est en contact prolong avec du chloroforme CHCH du dim thyle sulfoxyde DMSO ou du t trahydrofurane THF De plus il est attaqu par des acides concentr s tels que l acide sulfurique et l acide nitrique ou d un compos du hexane thyle ac tate et m thanol Ces acides peuvent cependant tre utilis s dans le cadre de proc dures de nettoyage condition que l exposition soit br ve e N utilisez pas de tubes PEEK cras s pli s ou abim s e Portez des lunettes de protection lorsque vous manipulez des capillaires en silice fondue pendant l installation d coupe etc e Avant d interrompre le fonctionnement pendant plusieurs jours ou plus observez les pr cautions figurant sur la page 34 e Utilisez des pi ces de rechange d origine Dionex Effectuer des remplacements par des pi ces ne provenant pas de Dionex ou utiliser des accessoires ne provenant pas de Dionex peut affecter les performances de l instrument e N utilisez pas l instrument de mani re autre que celles d crites dans ce manuel Page 6 Operating Instructions UltiMate 3000 Series SRD 3x00 Solvent Racks 1 3 Intended Use
52. vises against recycling the solvents This may impair the degassing performance Before connecting the solvent lines make sure that the connectors are free of contaminants Even minute particles can allow air to enter the degasser and thus reduce the degassing effectiveness When replacing solvents make sure that the solvents are miscible Mix immiscible solvents with an intermediate solvent for example isopropanol to replace them step by step Thoroughly rinse the degasser with alcohol after operation The alcohol does not need to be removed afterwards For longer periods of inactivity and when using saliferous buffers which may result in salt crystallization in the gas separation membrane thereby impairing the degassing performance rinse with de ionized water followed by either methanol or ispropanol Also observe the information about the solvent compatibility of the degasser page 27 Operating Instructions Page 29 UltiMate 3000 Series SRD 3x00 Solvent Racks 5 3 Operational Settings 5 3 1 Turning the Degasser on or off from the UltiMate 3000 System Pump When the SRD is connected to the pump of an UltiMate 3000 system and when the pump is powered up the SRD is powered from the pump The degasser is ready for operation You can turn the degasser on or off e from the front panel display of the pump e in Chromeleon if the pump is operated with Chromeleon e For routine on off control use the standby
53. voirs and appropriate tubing including frit holders with filter frits 4 3 1 General Notes When connecting the solvent reservoirs observe the following general precautions Observe the precautions for solvent selection page 27 Always install filter frits on the solvent lines This prevents contaminants from reaching the HPLC system that may increase wear and cause damage to the system Regularly check the filter frits for permeability This is especially important when using aqueous solvents Aqueous solvents may contaminate the filters with algae and other microorganisms that deposit on the filter frits Therefore also replace the solvents at regular intervals Rinse the reservoirs thoroughly before refilling them Replace the filter frits as necessary As a standard filter holders with 0 um stainless steel frits are provided in the accessories kit for the Solvent Rack Do not use these frits if the system includes a biocompatible pump or NCS 3x00RS Replace the stainless steel frits with the titanium frits from the accessories kit of the pump or NCS Open the filter holder and remove the filter frit When placing the new filter frit into the bottom part make sure that the frit is in a level position avoid tilting the frit Make sure that the tubing connecting the pump to the degasser is as short as possible and locate the solvent reservoirs as close as possible to the pump To avoid formation of gas bubbles in the reservoirs
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