Leica ASP300 User's Manual
Contents
1. Tuesday 13 February 2001 11 38 33 Adding changing reagents Highlight the desired station e Press REAGENT NAME e The SELECT REAGENT entry window pops up i e Highlight the desired reagent and press OK to confirm Only those reagents that are compat ible with the respective station will be available for selection 46 Fig 23 Allocating reagents e Only processing reagents except paraffin can be allocated to stations 1 10 e Only a cleaning reagent can be allocated to Station 11 e Only a cleaning alcohol can be allocated to station 12 e Only cleaning water detergent can be allo cated to stations 13 and 13 ext e Only paraffin wax can be allocated to the wax baths Instruction Manual V 1 3 12 2003 5 Operation 5 2 7 Reagent groups In this screen Fig 24 you find the colors used for each gt reagent group in the graphic program display Edit Reagent Groups Tuesday 13 February 2001 12 3923 T Reagent Group Name Colour 2 Dehydrating diluted m le J Dehydrating absoluta a 8 Cleaning waten detergent l ee ES Fig 24 Selecting a new color for a reagent group e Highlight the line containing the reagent color you wish to change e Press COLOR in the table header the entry window SELECT COLOR pops up e Select the desired color and press OK to confirm e Ifyou want to close the entry field without changing the color2. The duration of the wax clean program is user programmable Follow the instructions in Fig 37 61 7 Cleaning and maintenance 7 1 3 Smart clean 62 Smart Clean The Smart Clean prepares the ASP300 for transport Prior to starting a Smart Clean a complete retort clean program must be run To start a Smart Clean press SMART CLEAN on the MORE MENU FUNC TIONS screen and press OK to confirm The Smart Clean takes approx 15 minutes and consists of 3 phases Phase 1 Make sure all reagent containers are inserted correctly All internal tubing is cleaned with pressurized air Phase 2 Before phase 2 starts the operator is prompted to remove all reagent bottles from the instrument All internal tubing is again cleaned with pressurized air Phase 3 Before phase 3 starts the operator is prompted to insert an empty bottle into station 11 The tubing is cleaned with pressurized air for the third time remaining rests of reagents if any are forced back into station 11 ing them into the instrument to make sure there will be no re agent spills from the bottles that could cause damage during transport A Empty and clean all reagent bottles thoroughly before reinsert The SMART clean is ideal for removing residual liquid from the internal pipes of the instrument as it thoroughly purges them with pressurized air A SMART clean should be carried out once every month Instruction
3. Leica ASP300 Advanced Smart Processor Automated vacuum tissue processor Instruction manual Leica ASP300 V1 3 English 12 2003 amp Always keep this manual together with the instrument CLCa Read this instruction manual carefully before working with the instrument MICROSYSTEMS NOTE The information numerical data notes and value judgments contained in this manual rep resent the current state of scientific knowl edge and state of the art technology We are under no obligation to update the present manual according to the latest tech nical developments nor to provide our cus tomers with additional copies updates etc of this manual For erroneous statements drawings techni cal illustrations etc contained in this manual we exclude liability as far as permissible ac cording to the national legal system applicable in each individual case In particular no liabili ty whatsoever Is accepted for any financial loss or consequential damage caused by or related to compliance with statements or other information in this manual Statements drawings illustrations and other information as regards contents or technical details of the present manual are not to be considered as warranted characteristics of our products Published by Leica Microsystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Telephone 49 0 6224 143 0 Fax 49 0 62 24 143 200 Internet http www histo solution
4. Leica ASP300 55 6 Trouble shooting 6 1 General The Leica ASP300 Is equipped with a trouble shooting system lf an error is detected detailed error messages and corresponding user instructions on what to do are displayed on the touch screen For all correctable error conditions the instrument will take corrective actions to protect the specimens PE e Details of all events are recorded in Run Log Run log can be viewed by pressing MENU and then RUN LOG 6 2 Power failure Power failure during a processing program e Inthe event of a power failure the instrument automatically compen sates any impact the power failure might have on the specimens Power failure during a paraffin or retort clean cycle e fa power failure occurs during a clean cycle the total duration of the cycle will be extended by the duration of the power failure 56 Instruction manual V 1 3 12 2003 6 Trouble shooting 6 3 Trouble shooting e Check for error messages Indicating the nature of the problem e Press the Help button on the message for any information relating to the cause tg e Check the Run Log for any information on the nature of the failure i Use the SMART SCREEN functions to individually test opera tions such as fill drain pressurizing and evacuation operations Power failure e Verify thatthe mains lead is plugged in to the wall socket and that if applicable the wall soc
5. press CANCEL Leica ASP300 47 5 Operation 5 3 Programs Programs can be started from two different screens FAVORITES max 5 programs Favourites Tuesday 13 February 2007 10 25 03 Day Run Small gt Biopsies Finish ASAF Fig 25 A Favorite is a processing program that is frequently used All settings are already programmed Only the number of blocks has to be entered if this function is enabled Starting a Favorite To start a Favorite press the corresponding symbol on the Favorites screen the program will be started immediately 48 ALL PROGRAMS All Programs Tuesday 13 February 2001 10 34 12 Routine D Overnight 6 High Priority Program 2 Day Run Small P Very Fast Biopsies B Test step Fig 26 In the Favorites screen press ALL PROGRAMS All Programs Press the symbol corresponding to the pro gram you wish to start A graphic representation of the stations retort will be displayed with the stations re quired for the program you are about to start colored in the corresponding reagent group colors Fig 27 Prior to starting it modify the program as required All processing step modifications made will only be applied to the current program Instruction Manual V 1 3 12 2003 5 Operation 5 3 1 Editing a selected program ee eee eee y Redefine the program finish time za e Press FINISH TIME In the entry window set Se a n
6. ASP300 touchscreen press the corresponding button symbols Examples of buttons are Clean Screen Buttons on the Leica ASP300 touch screen can either be enabled or dis abled A button that is disabled appears with a thinner border than a button that is enabled Instruction manual V 1 3 12 2003 4 Setting up the instrument 4 7 2 Access control The Leica ASP300 may be configured to allow two levels of user ac cess Operator symbol Operator access level e Operators may run programs and view results On this level the OP a ERATOR symbol ts displayed in the upper right corner of the touch screen Supervisor symbol Supervisor access level e Supervisors may perform all Operator functions and additionally cre ate programs and perform the Instrument sit up functions e To access Supervisor level press SUPERVISOR enter the required password and confirm The SUPERVISOR symbol will then be dis Soera played instead of the OPERATOR symbol and all enabled buttons will be surrounded by a black border instead of a blue one u Lock feature against unauthorized use e The Leica ASP300 provides a feature called Lock to prevent ac cess to any operations of the Leica ASP300 to unauthorised users e The Lock mode and the corresponding Lock password are config ured from the SYSTEM SETUP menu Leica ASP300 27 4 Setting up the instrument 4 8 Check list for first operation at su
7. After a wax bath has been filled tt must be marked as full in the REAGENT STATUS screen Software control e Ifthe wax baths are filled with molten wax it is possible to override the Leica ASP300 melting time calculation For that purpose in supervisor mode access the SERVICE FUNCTIONS screen Fig 16 Service Functions Pe E ia e Press TIME UNTIL WAX MELTED WARNING The functions below should only be used by certified Service Staff Incorrect use of Enter the req ulred delay time and Press OK these functions can cause the instrument to malfunction z to confirm Reset Primier inae Miadi Wiii Bod Malton inne Last Retort Cleaning Solvent Reagent cscs Fig 16 If the instrument attempts a paraffin step with the paraffin only partly molten severe instru ment malfunctions may occur Leica ASP300 33 5 Operation 5 2 Setting up the instrument parameters From the start screen press MENU to go to the MENU FUNCTIONS screen then press MORE In the MORE MENU FUNCTIONS screen press SYSTEM SETUP stem Setup 5 2 1 System setup Monday 23 April 2001 09 28 46 G SYSTEM SETUP screen Program Options Display Buzzer Date Time Fi g l 1 7 Station LCD Backlight 4 Li 1 Wax Bath Onder Auto 09 28 46 LCD Contrast Prompt for Enabled 1 Buzzer Volume Security Instrument Modus Lock Mode Enabled Fig 17 Display Buzzer The DISPLAY BUZZER field
8. Leica ASP300 43 5 Operation 5 2 5 Favorites maximum of 5 programs Favorites can be programmed to end either e as soon as possible ASAP or e ata predetermined finish time ViewlEdit Favourites E Monday 05 March 2001 12 11 10 nmm Name Routing Lvernight Progra 1 e Adding and or modifying Favorites e Highlight the program line to be altered e Press PROGRAMS e Select the desired program and press OK to confirm Setting a finish time e Press FINISH TIME e Enter the finish time 24 hour clock Select ASAP if the program finish time is not to be delayed e Press OK to confirm 44 Instruction manual V 1 3 12 2003 5 Operation Delete Leica ASP300 Assigning a finish day e Press DAY e Select the desired week day in the list and press OK to confirm Assigning an icon to a Favorite e Press SYMBOL in the table header e Select the desired icon and press OK to confirm The first icon on the list is empty select this option if you do not want to assign an icon to a specific program Deleting a Favorite e Highlight the Favorite to be deleted e Press DELETE i The Favorite is deleted from the FAVORITES list only the program itself is not deleted 45 5 Operation 5 2 6 Editing the stations Fig 23 shows all stations allocated reagents cur rently defined in the instrument ViewEdit Stations
9. Remote Then press STATION DRAIN Remote Station Drain e Several queries will be displayed each of which has to be confirmed individually Leica ASP300 31 5 Operation Filling with wax pellets Set as Full Cycles Daye 2 Blocks Cyclaa Mays eee Slace Since Sinca Since Simce Sinius Changed Eime Changed Ohaned Cleaned Cleaned se en E E E E E E pae ferea pei S tue L aE A bid Paran Votan a ae E Fig 15 A Remove the splash protector Fill the wax bath Fig 15 with wax pellets up to the top Press BACK and REAGENT STATUS to ac cess the reagent status table Fig 14 High light the wax bath you just filled and press SET AS FULL After about 90 minutes add another 0 5 kg of wax pellets The pellets will take a total of approx 10 hours to melt Important During the melting process the paraf fin level MAY NOT drop below the MIN level 2 Fig 15 Once the paraffin is completely molten re insert the splash protector Software control The software calculates when the wax will be ready for use and selects program start ing times accordingly i e the paraffin will definitely be molten when it is required for the first paraffin step Instruction Manual V 1 3 12 2003 5 Operation Adding molten paraffin e Molten wax may have a maximum temperature of 70 C e ffilling with molten wax do not fill above the MAX level see Fig 15 e
10. an alarm situation occurs the circuit is closed The remote alarm device connected to the instrument must be rated at less than 2 amp A maximum voltage of 30 V DC may be present Connecting a remote alarm device A remote alarm device can be connected to the Leica ASP300 via a 6 3 mm diameter jack connector optional accessory e For details on how to connect a remote alarm device to the Leica ASP300 please contact your local Leica office or the manufacturer Leica Microsystems Nussloch directly 23 4 Setting up the instrument 4 6 Switching the instrument on Once the instrument has been switched on the mains switch ON OFF 5 should always re main in position ON e After switching it on the instrument will take a few minutes to initialize see opposite Initialising the instrument Fi g 7 i e After initialization the FAVORITES screen will be displayed Fig 8 Screen saver e A screen saver will operate turning the screen off when no key has been pressed Rome ovem for a user programmable time L Press any part of the touchscreen to restore Day Run Small the screen Biopsies Finish ASAP After having been restored the screen will be inoperational for a few seconds to avoid accidental activation of any key symbols Very Fast Fmi ASAF 24 Instruction Manual V 1 3 12 2003 4 Setting up the instrument Monday 05 March 2001 07243 1 The following f
11. content of the concluded agreement The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively Technical service information lf you require technical service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information e Model name and serial number of the instrument e Location of the instrument and name of the person to contact e Reason for the service call e Date of delivery Decommissioning and disposal Leica ASP300 The instrument or parts of the instrument must be disposed of in compli ance with the local laws 69 Notice 70 Instruction manual V 1 3 12 2003
12. is practically self explanatory ee e The number next to DISPLAY OFF indicates the remaining time in min 7 until the screen saver after the last user action is activated av e LCD CONTRAST can be set from 0 to 14 La nas e The BUZZER VOLUME can be set between 1 and 10 Buzzer Volume 34 Instruction manual V 1 3 12 2003 5 Operation Program Options Sequential 1 Wax l 2 Wax ll 3 WaxiIll Prompt for Disabled Num Blocks Wax Bath rer Date Time Date 13 02 01 Time 12 45 42 Instrument Leica ASP300 Program Options Selection of the options on how programs are carried out e STATION ALLOCATION By age or Sequential By age RMS ts activated reagents will automatically be used in order of increasing cleanliness Sequential reagents will be used in sequential order of stations e WAX BATH ORDER Auto or 1 2 3 Wax bath order can be activated only when STATION ALLOCATION is set to Sequential e PROMPT FOR NUM BLOCKS Enabled or Disabled Enabled When starting a program the actual number of blocks must be entered This is recommended with the RMS being enabled Disabled Number of blocks can be entered optionally not mandatory Date Time Be sure to verify that the date and time entered do actually correspond to the local time date Press the buttons to access the entry windows where time date can be corrected if necessary Instrument Press
13. min System setup Password status Type of password Reagent Management Status Software access control Lock Mode 16 Supervisor User alphanumeric freely selectable ON OFF ON OFF Instruction manual V 1 3 12 2003 3 Instrument components and specifications 3 4 Technical Data continued Hardware and Software Large color liquid crystal display LCD and touchscreen User friendly intelligent software 3 5 floppy drive and printer port Alarm system with two remote alarm sockets Password protected instrument supervisor mode Multiple specimen protection system Capacities Leica ASP300 15 programs of up to 10 reagent and 3 paraffin wax steps each e Time per program step 0 to 99 hours 59 minutes e Delay time max 7 days Up to 300 cassettes can be processed simultaneously Four user programmable retort clean programs Wax clean program 10 Reagent bottles 3 Wax baths 3 Cleaning solution bottles 1 Condensate bottle Reagent temperature adjustable from 35 C to 55 C or ambient Paraffin temperature adjustable from 40 C to 65 C Choice of three retort drain rates of 80 120 and 140 seconds Up to 100 reagent names in memory 17 3 Instrument components and specifications 3 5 Compatible reagents 18 Fixatives 1 Formalin buffered or unbuffered 2 Formalin saline Dehydration 1 Ethanol 4 Butanol 2 lsopropanol 5 Industrial Methylated
14. name and allocate the new reagent to the reagent group it belongs to 2 Define the stations bottles that will be filled with the new reagent 3 Fill the stations bottles according to the reagent list Enter reagent names Enter reagent names via the REAGENTS screen You must be logged on at supervisor access level to proceed e From the start screen press MENU to go to the MENU FUNCTIONS screen then press MORE to go to MORE MENU FUNCTIONS e Press REAGENTS e The SET UP REAGENTS AND WARNING THRESHOLDS screen opens e To add a reagent e Press INSERT e Enter the new reagent name e Press OK to confirm e You will then automatically be prompted to select the reagent one Cycles TE maci Geles F i Reagent Group since since since m oh Champel Cleaned Cloned Ceased Dehydrating absoluta Allocate the new reagent to the desired group and press OK to confirm Fig 19 Leica ASP300 37 5 Operation compatibility monitoring Allocation to the wrong reagent group can lead to reagent cross contamination A Allocation of reagents to the correct reagent group is the basis for Changeable parameters Entering modifying reagent thresholds Blocks Cycles Days e Highlight the reagent to be modified since since since Changed Changed Changed Press the headline key symbol of the parameter to be changed e Enter a new threshold or if no warning Is desired press CLEAR to remove
15. on the title page A name plate indicating the instrument serial number is attached to the back of the instru ment Leica ASP300 This instrument has been built and tested in accordance with the following safety regula tions on electrical measuring control regulat ing and laboratory devices e DIN EN ISO 9001 e EN 61010 1 e EN 61010 2 e EN 61326 1 and EN 61326 2 e EN 61000 3 2 and EN 6100 3 3 The protective devices on both in strument and accessories may neither be removed nor modified Only authorized and qualified service personnel may repair the instrument and access the instrument s internal components 2 Safety 2 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention Primarily responsible for accident free operation is above all the institution which owns the instrument and in addition the designated personnel who operates services or repairs the instrument To ensure trouble free operation of the instrument make sure to comply with the following instructions and warnings Transport and installation Warnings and instructions on transport and installation of the instrument can be found on a separate information sheet attached to the outside of the instrument s shipping crate Warnings reagent handling A To avoid damage to the instrument only use the reagents listed in chapter 3 5 When handling re
16. protection as an additional electrical safeguard Make sure to use the appropriate mains cable for the local volt age supply wall outlet e Connect mains cable 6 to mains power supply socket 1 see Fig 3 e Jumper cable 3 connects the mains power supply output 2 to the electronics module input 4 e Switch ON OFF switch 5 ON e Switch ON STOP switch atthe right of the instrument ON Electrical connections at the _ rear panel of the om 7 instrument E Q eg7e2e2s328393939329 o 4 x l 7 MATII A gt Cables UU connected Wt Fig 3 correctly eaeeeeee8 4 800800808 a A 0 6 A D a 9 Important Specification for connection 2 is as follows 100 120 V or 230 240 V max 200 VA 20 Instruction manual V 1 3 12 2003 4 Setting up the instrument Fig 4 Leica ASP300 4 3 Installing the accessories Unpack the activated carbon filter and in sert it into the instrument see Fig 4 Make sure to insert the filter with the correct side facing upwards See label 6 at the front of the filter the arrow must point upwards Unpack the magnetic stirrer and insert into the retort Install the console flap covers floppy drive and ports Prior to filling the bottles with reagent for the first time check the connections If neces sary tighten manually Remote fill drain hose Co
17. specimen cassette baskets may be used with or without cas sette dividers and spring separators designed to separate cassettes and improve fluid penetration In process fluid recirculation tidal agitation for continuous and ef ficient mixing of fluids Remote fill drain system for reagents Remote fill drain system for paraffin Automatic optical fluid level sensor Active paraffin wax solvent removal feature Magnetic stirrer Programmable program start time delay Programmable finish time for processing programs 3 step retort drain feature Specimen processing under pressure vacuum alternating pressure vacuum cycles or under ambient pressure User programmable clean cycles 11 3 Instrument components and specifications 3 2 Overview instrument components ST nit it 4g A san an j m h 3 a nes a wf if i it i Hi Si ab ji Ae E l pop lg ii r St lt eee zi 4 Z es il f n d di We Fig 1 12 Instruction manual V 1 3 12 2003 3 Instrument components and specifications Overview Instrument components ao eo OO C1 Aa GW N I N e m oe eo OO C1 FS GW HB a O I I I I I 1 I I I 1 I I N 1 N N GS NY 1 I Basic instrument processor module Basic instrument reagent module Wax bath lids Retort lid Touch screen Cover flap of instrument console Instrument console comprising Prin
18. Inspect and if necessary clean wax bath lid seal Inspect and if necessary adjust paraffin levels Inspect reagent container fluid levels if the automatic fill and drain function is actually being used Leica ASP300 67 7 Cleaning and maintenance Maintenance activity daily weekly monthly every 4 months Inspect and if necessary y clean wax bath strainers Inspect wax bath air vent and y if necessary clear Inspect and empty condensate bottle y Clear inlet holes Examine activated carbon filter y Replace carbon filter y Remove fluid level sensor float inspect and clean y Inspect electrical connectors y at the rear panel of the instrument Inspect and if necessary y clean inside reagent bottles Check that the bottle caps and seals y O rings of the reagent bottles are in place and tight Check that the reagent bottles are fully y inserted in the connection manifolds 68 Instruction manual V 1 3 12 2003 8 Warranty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality con trol procedure based on the Leica in house testing standards and that the product is faultless and complies with all technical specifications and or agreed characteristics warranted The scope of the warranty is based on the
19. ME The colors on the left border of the table indicate the reagent groups to which the reagent of the step belongs The program steps max 13 steps per program are displayed in the order in which they are carried out The following characteristics can be edited Leica ASP300 reagent name duration of step with the exception of fill and drain steps retort temperature if Ambient is selected the retort temperature indication remains empty type of pressure and or vacuum cycle retort drain time delay step 41 5 Operation Editing program steps To edit a step highlight the corresponding line and press the re spective headline In the entry windows that pop up enter select the program step set tings 42 Instruction manual V 1 3 12 2003 5 Operation Editing program steps continued Setting a delay step A so called delay step ensures that a program is finished at a specified Delay time 7 e Highlight the program step you wish to define as a delay step e Press DELAY The delay symbol is moved to the selected step thus defining the step as delay step Copying steps e Highlight the step to be copied e Press COPY e Use the MOVE UP MOVE DOWN symbols to move program steps up or down within an existing program without having to recreate those steps Move Up hove Down DELETING PROGRAM STEPS e Highlight the step to be deleted Delete e Press DELETE
20. Spirits 3 Methanol Clearing 1 Xylen recommended 2 Toluol Prior to using these reagents contact 3 Chloroform Paraffin wax your Leica representative or Leica di rectly for information on necessary pro tective measures Using these reagents 1 Paraffin will require an increased number of main tenance interventions by Leica Techni cal Service Caution Reagents other than those listed here may damage some com ponents of the instrument Do not use acetone benzene or trichlorethane in the instru ment Fixatives containing mercuric salts acetic or picric acid will corrode metallic components in the instrument and shorten in strument life If you choose to work with such fixatives it is essential to per form a clean cycle which contains multiple water rinses each time after use to minimize damage In addition we recommend frequent and regular preventive maintenance by the Leica Technical Service Instruction manual V 1 3 12 2003 4 Setting up the instrument 4 1 Site requirements e The instrument needs approx 650 x 700 mm floor space e Practically vibration free floor e Room temperature constantly between 15 C and 35 C e Relative humidity maximum 80 non condensing e Avoid vibrations direct sunlight and heavy variation in temperature The chemicals to be used in the Leica ASP300 are both flam mable and noxious Therefore the instrument must be
21. WAX BATH SET TEMP C to access the wax bath temperature entry window Select the highest temperature compatible with the type of paraffin used as paraffin temperature will drop slightly once the paraf fin is in the retort 35 5 Operation Security To determine the level of access to the instrument e SUPERVISOR PASSWORD Enabled or Disabled _ Enabled A password is required for access to the supervisor level Enabled Set Pwd Lock Mode Enabled Disabled All instrument and software functions are fully accessible to all staff e LOCK MODE Enabled or disabled Enabled No screen functions can be accessed To unlock a password has to be entered Disabled Screen can be accessed at any time until the screen saver turns off the screen Entry keyboard The keyboard is displayed any time text needs to be entered The key board headline 1 tells you what kind of text to enter 30 characters can be entered into each entry field though sometimes not all characters entered can be displayed Important keys Shift a a B 4 o 6 e E o i To shift to upper case symbols AltGr aff S S S S ole aa For access to a number of special characters 000000000 Bn _ 2 B le on m a ES Deletes last character entered one Clear Deletes entire line ok Fig 18 36 Instruction manual V 1 3 12 2003 5 Operation 5 2 2 Editing the reagent list Adding new reagents 1 Enter the reagent
22. Z Syste M SeN enea a a 34 522 Ed ngthereagentlis t ensanena a A NS 37 D29 VIEWING the program liS Ttime AO REANA 40 5 2 4 Adding and or modifying programs cccccesessesssssssssscsececsrsessesesrsseseesessnsensasensensaneaees 41 5295 Favorites MAXIMUM Of 5 Programs sscssisvatcsiscvinseisavasivetachannd asasnelvatiuiudadineutiNatetindaia 44 4 Instruction manual V 1 3 12 2003 Table of contents Leica ASP300 D260 EGIL G THC SLA LIONS menkes A AAE RRES 46 5 2 7 Reagent QrOUPS cccceccesssccscssssssssssscesessucecsesseseeseeseseesensecseseusausessesenseesesseseusensassnsensansesenseneates 47 5 3 PrO gt 1 Kc a Ce en ee err 48 531 VEGIUNG a selected Progra sariani a T 49 532 olanda PlOU ball aiauecd sinciavantceciactsinctecunsatasdeunaatienueaaesaneniianduientonadaamatena aaa danmenaete 51 5 4 Reagent STATUS oc ceccccccssscsecsesscsessecsesessnseececseseeseesesseseesessesensensessesenseesensesensensesnsensassesneaneess 52 5 5 DV SCOT MONIO ananena a baseiomeesieneutaea 53 5 6 On line REl pretest eerie ene sc PO nS nee re eee 54 5 7 GOnTI UrINgG a DENLEF ODO NA errenneren aaraa AN RTE RR 55 6 Trouble SNOIN sasis a aaa 56 6 1 Gener atl sca sevaateccyten aa ancaed aah nana tetcael atseasadustedorauu manatees 56 6 2 FOO Eero hat tna ar hte ate ven aah caeaiguta ayy es Datanteaneanarad ee siesuuaueiner Nts 56 6 3 Trouble Shootin gouienn na N Roane 57 6 4 Typicaltilor drain DroDIEMS iectsecunseleworehandediediaitebuieciniteaus
23. agents follow the instructions of the manufacturer and make sure all laboratory regulations in force in your country are complied with Dispose of waste solvents with care according to local regulations and the waste manage ment policy of the company or institution Use caution when handling hot molten paraffin Do not touch paraffin stations and retort walls they can be very hot Do not clean the reagent bottles in an automatic dishwasher Material safety data sheets can be obtained from the supplier of the chemicals They are also available on the Internet http www msdsonline com g Instruction manual V 1 3 12 2003 2 Safety Warnings cleaning and maintenance A Prior to each maintenance switch the instrument off and disconnect mains power Do not clean the instrument with solvents containing acetone or xylene No liquid may be spilled into the internal components of the instrument Empty the condensate bottle at regular intervals Warnings working with the instrument The Leica ASP300 may only be operated by trained laboratory personnel and as per the present instruction manual In case of emergency switch off mains item no 5 in Fig 3 at the rear of the instrument and unplug the power cord The instrument must be installed so that the mains switch at the rear of the instrument can easily be accessed at any time Always press PAUSE before opening the retort lid during pr
24. ce Status Full In the reagent status screen Fig 32 you can display and update the actual reagent status of each station and you can set the stations e Warning thresholds that trigger warning messages appear on a red background Full eons toa E EEs a JABSIMS O o O IMS w2 5 e m TE 2 M Ea i ABS IMS 516 4 Ful 7 asses ee eh C fisne wm e pe o o CE A a foua E oe ep Peon en ray 3 Ce Reagent warnings If the RMS is enabled the instrument will give you warning messages when reagents have been used for too long Warning messages for normal reagents are given based on up to 3 criteria warning mes sages for paraffin wax on up to 6 criteria The warning messages are displayed at the end of a cleaning cycle From there you can go directly to the REAGENT STATUS screen In addition warning messages on a yellow background are displayed in the program start screens 52 Ful m e To change a reagent status highlight the corresponding line and press the corresponding key symbol Fig 32 Criteria for warning messages For normal reagents the following warning cri teria apply e number of blocks processed e number of programs carried out e number of day lapsed since the reagent has last been changed For paraffin wax three additional criteria can be applied e number of blocks processed e number of programs carried out e number of days
25. deantshddilvets siaduasheie Avenida budselteiaenst 58 7 Cleaning and maintenance sie sess szisiscs sacsascascaciaccaaishdescticanssssndeatusiaansussinnscisanatvouachcdnataaveiuanenacs 59 7 1 Cean modras size tcssalecinaressasersush anecsasaectasaussunsubineaieasadeacsuneactacneusweatuceneaseastucniersaakauaanausenmatieaaes 59 Flek AXETOE CC ati Drogi AIS eraann ana N 59 REZ Wax Cle ath DOG GINS apenn aa 61 1 1 3 Smart clean i sccssiscaziasavecaiaavaysasndeasetnaiatdonanacesagueadioavallantaglatognaubcghain ina aehetonjastarsoleasiaiaaueeiernniaieas 62 7 2 Ged Aven ei ees Ceire HAH 1S STEDS ee Re en ee Rn oe ee ee ee 63 7 3 1 Daily Cleaning and Maintenance saienisi T 65 7 3 2 Periodic ClEANMING ANA MAINTENANCE ceceecessesssessssecsessncsscessnsenseesessnseesesersensensasensensanes 66 7 4 Preventative Maintenance schedule cccesssccssssssessesrssessesesseseessneesesenseesetenetseeess 67 8 Warranty and service ccssssssssssssssssseeseessessessecsesecsesaesaesaesaesaesseeserseesersersessessersesseesneess 69 1 Important notes 1 1 Symbols used in this manual and their meaning gt Bp gt 5 Warnings appear in a grey box and are marked by a warning triangle Notes i e important user information appear in a grey box and are marked by an information symbol i Flammable solvents and reagents are marked with this symbol Instrument surfaces which beco
26. emer E Press BACK 2 to go back to the screen o o from where you accessed Help MAPICS CUSTOM HELP 3 is only displayed if user specific help files have been installed Wiha ia a favorite A favorie allows to quick steet a program Smart Start A favorite is a processing program that optionally has predetesmined finieh bine and day also includes the appropriate run oplions eg pretest agitation fic and appears om ihe Faenrbes form To sei up a program as a fronte seo the Edi Favores form Fig 34 Detailed information on how to create and install customer specific help files can be found in the What is Custom Help help text Accessing Help in the event of error messages e Press DETAILS or e Press the HELP button on the error message 54 Instruction Manual V 1 3 12 2003 5 Operation 5 7 Configuring a printer optional B To select and configure a printer proceed as follows gt Installation e With a Centronics standard cable connect the printer to Printerlype the printer port refer chapter 4 4 Fig 6 More Printing run logs Printing is possible when the PRINT symbol is displayed Print fa For the PRINT symbol to be displayed a printer must have been configured for the instrument The following lists and protocols can be printed e reagent list e stations list e reagent status list e all programs e run log e error log
27. ew finish time Finish Time JEBB Thursday 26 April 2001 08 50 Fig 27 To modify a program prior to starting it press Thursd PE EDIT to access the Edit Program screen oor Fig 28 Eda Routine Overnight Program ENE SIAF i e Enter the day when you want the program Fomai 7 P e Enter the finish time 24 hour clock format nsus majaz fev ssm onan ov oo e Press OK to confirm ABS IMS os wv ew ate te pees feja few e _ esms eo ww ew wo five es oe ew oo me e ow ew om fe es few oe Porat too ee ev va emm vefe w Proviso e few wo Fig 29 Leica ASP300 49 5 Operation Access the RUN OPTIONS function to display O a the options selected for the current program Run Options Disabled Cancel Fig 30 LEVEL CHECK enabled The lower level sensor in the retort is acti vated RECIRCULATION enabled During each processing step the reagent is pumped periodically from the bottle to the retort and back for continuous and efficient mixing of fluid The first pump step is carried out after 16 minutes all subsequent pump steps in 12 minute intervals PRETEST enabled After starting the program a test run Is per formed the first reagent is pumped into the retort and back into the bottle STIRRER enabled Magnetic stirrer Is on PRINTER enabled At the end of the program run a full
28. ill or drain lf the retort is empty Run the extended retort clean program Use the maximum set tings for number of clean cycles and temperature consistent with the type of reagents solvents used in the clean program listed steps call Leica Technical Service Instruction manual V 1 3 12 2003 7 Cleaning and maintenance 7 1 Clean programs You find the clean programs at the end of the programs e re gt list Fig 35 To display a clean program highlight the re T spective line and press EDIT The ASP300 offers three retort clean programs and one paraffin clean program Clean programs cannot be copied or deleted 7 1 1 Retort clean programs Oo 2 B j SE 23 October 2001 10 41 54 00 48 enviar 2 oe 1 Cleaning Solvent 1 step from station 11 2 Cleaning Alcohol 1 step from station 12 3 Cleaning Water 1 step from station 13 A clean program comprises a minimum of three and a maxi mum of four steps Optional step between steps 2 and 3 Cleaning Water Detergent 1 step from station 13 ext Fig 35 e The steps of retort clean programs are listed in the order in which they are carried out e The clean programs skip any steps not required to complete the re tort clean Leica ASP300 59 7 Cleaning and maintenance Editing steps 60 To edit a program step highlight the corresponding line and press the respective table header To disab
29. ket is switched ON e Verify thatthe jumper lead or UPS lead are fully inserted into their re spective socket e Verify thatthe power switches on the rear adjacent to the power lead and on the side of the instrument are both switched ON lf by carrying out the above listed steps you cannot identify the problem call Leica Technical Service Leica ASP300 57 6 Trouble shooting 6 4 Typical fill or drain problems 58 Fill and drain problems may be due to several causes 1 There is insufficient reagent Verify that both the reagent bottles and the wax stations have sufficient reagent 2 Insufficient pressure or vacuum The Leica ASP300 is unable to create sufficient vacuum for a fill or pressure for a drain in the retort Verify that the reagent bottle in question is correctly inserted in the reagent cabinet Verify that all reagent bottles are fully pushed home into their sockets Check whether there is some debris under the retort lid seal causing a leak 3 There is a blockage in the air or reagent lines lf there is reagent in the retort that cannot be drained try to remove the blockage as follows i If you cannot eliminate the problem by carrying out the above Heat the retort to the maximum possible temperature consistent with the reagent currently in the retort if any and leave it at this temperature for at least 15 minutes Whilst the retort is at maximum temperature attempt to f
30. lapsed since the wax has last been cleaned Instruction Manual V 1 3 12 2003 5 Operation y e aes The SYSTEM MONITOR screen supplies important infor mation on the Leica ASP300 system status System Monitor Y PU M P AG E Indication of hours of service of the pressure vacuum pump After approx 1 000 hours of use have Leica Technical Service carry outa preventive maintenance of the pump CARBON FILTER AGE Indication of hours of service of the activated carbon filter The carbon filter must be ex changed after approx 4 months of use 5 5 System Monitor Fig 33 After replacing the filter press RESET Reset ROTARY VALVE RETRIES lf a figure higher than 50 is indicated a preventive maintenance is recommended FILL DRAIN This screen displays the average fill drain times of all stations in seconds Fill Drain Short term average duration of last 5 fills drains Long term average duration of last 20 fills drains lf one of the figures displayed exceeds 250 call Leica Technical Service Leica ASP300 53 5 Operation 5 6 On line help The Leica ASP300 features an extensive on line Help system For information on basic problems refer to the Help Index to access press INDEX on the Help screen Help What is a favourite Thursday 26 April 2001 14 24 59 Use the UP DOWN arrows to scroll Press PREVIOUS TOPIC 1 to sec a acca a return to the index A ei
31. lastic scraper provided Make sure to remove all wax from around the retort lid seal e For convenience the lid may be removed during cleaning by lifting it vertically and sliding it towards the console Cleaning the retort e The retort may be wiped clean using a cloth moistened with solvent xylene or alcohol or mild detergent Ensure that the air holes at the top front of the retort are open remove all dirt Cleaning the retort strainer e Use alcohol or xylene to clean the strainer located at the bottom of the retort For convenience the strainer may be taken out to remove all solid dirt Cleaning the touch screen e On the MENU FUNCTIONS screen press CLEAN TOUCH SCREEN e Clean the touchscreen e Press ENABLE EXIT and then BACK to return to MENU FUNCTIONS Cleaning the processor module top surface e Remove both lids for cleaning e First scrape the stainless steel surface with the plastic scraper and then wipe down Reagent and condensate bottle seal lubrication e To ensure easy removal of the reagent and condensate bottles lu bricate the O ring seals on the plug in nozzles with the O ring lubri cant supplied e Check regularly whether the reagent bottles are still properly con nected to the connection manifolds in the instrument Retighten If necessary 65 7 Cleaning and maintenance This procedure is particularly important with bottles containing chloroform Chloroform tends to swell the bottle sea
32. le a step set the number of cycles to 0 Only cleaning wa ter steps can be disabled The following characteristics of each step can be edited Reagent name only those reagents that are compatible with the re agent station being edited will be displayed Number of cycles the duration displayed is calculated based on the number of cycles Retort temperature temperatures from 50 to 65 C can be set Station 13 ext The term 13 ext stands for an additional station which can be used instead of station 13 Station 13 always contains cleaning water lf a program contains a 13 ext step during the clean cycle the op erator is prompted to insert bottle 13 ext when needed and to re move the bottle as soon as the step has been completed located to station 13 ext i Only reagents belonging to the cleaning water group can be al Station 13 ext may be used for clean programs only Instruction manual V 1 3 12 2003 7 Cleaning and maintenance 7 1 2 Wax clean programs hen Wax Clean Ill Rapid Cleaning Last cleaned 180401 program Wax Clean Il Standard Cleaning Last cleaned 18 04 01 Program Wax Clean I Extended Cleaning Last cleaned 250401 program opori Leica ASP300 Fig 37 Fig 36 The Leica ASP300 contains a wax clean fea ture that removes solvents xylene from used wax Follow the instructions on the left Fig 36 to run a wax clean program
33. ls making them difficult to remove This problem can be prevented by regular lubrication Checking the retort and wax bath lid seals e Regularly check the retort and wax bath lid seals for damage If a seal is damaged It must replaced without delay 7 3 2 Periodic cleaning and maintenance Cleaning the reagent bottles e Remove the reagent bottles from the reagent cabinet Empty and clean them using a bottle brush and a laboratory deter gent in warm water e Never clean the reagent bottles in an automatic dishwasher e Wipe the stainless steel inner walls of the reagent cabinet with a damp cloth moistened with a mild detergent e Refill and reconnect the bottles once cleaned Make sure the bottle lids are tight e Make sure the bottles are properly pushed home into the connection manifolds at the rear inner wall of the reagent module 66 Instruction manual V 1 3 12 2003 7 Cleaning and maintenance 7 4 Preventative maintenance schedule Maintenance activity daily weekly monthly every 4 months Lubricate reagent bottle O rings and check for damage y Wipe dry retort and lid after completing retort clean cycle Clean exterior of instrument with soft cloth and minimal amount of xylene Check retort strainer for any tissue or wax debris Remove residual paraffin from inner surface of wax bath lid Inspect and if necessary clean retort lid seal
34. manual V 1 3 12 2003 7 Cleaning and maintenance 7 2 General cleaning steps Paraffin wax baths Warning Be careful as the walls of the wax baths are very hot and may cause burns e Wipe the wax baths and lids clean The lid can be removed for cleaning purposes e fthey are dirty remove the wax strainers from the wax baths Clean dry and reinsert them e Ensure that the air vent hole at the top rear right hand corner is un obstructed Instrument exterior Clean the instrument exterior as necessary Wipe with a damp cloth moistened with mild detergent and dry Do not use solvents on painted surfaces and or the touch screen Checking the drip tray Regularly check the drip tray for spilled reagents refer Fig 38 Fig 38 Leica ASP300 63 7 Cleaning and maintenance Draining the condensate bottle e Remove and empty the condensate bottle and reinsert Fig 39 e Exchange the filter as shown in Fig 40 e Replace the filter at least every four months make sure to Insert correctly see arrow on label e Perform a Smart Clean prior to changing the filter Fig 40 T U Penance THIS FILTER ON LEICA PART NO 0476 34150 Filter label 64 Instruction Manual V 1 3 12 2003 7 Cleaning and maintenance 7 3 1 Daily cleaning and maintenance Leica ASP300 Cleaning the retort lid e Remove wax from the inside of the retort lid with the p
35. me hot during operation are marked with this symbol Figures in brackets refer to item nos in drawings Function keys to be pressed on the instrument touch screen are written in bold print capital letters 1 2 Designated use The Leica ASP300 is an automated tissue pro cessor for the following laboratory applications e fixation e dehydration e paraffin wax infiltration of histological tissue specimens The Leica ASP300 must be operated exclu sively with the reagents listed in Chapter 3 5 Compatible reagents Any other use of the instrument is considered improper Instruction Manual V 1 3 12 2003 2 Safety A use of other Leica products Make sure to comply with the safety instructions and warnings in this chapter Make sure to read these instructions even if you are already familiar with the operation and 2 1 Safety instructions This instruction manual includes important in formation related to the operating safety and maintenance of the instrument and It is an im portant part of the product If additional requirements on accident prevention and environmental protec tion exist in the country of operation this instruction manual must be supplemented by appropriate instruc tions to ensure compliance with such requirements i Instrument type All information provided in this manual applies only to the instrument type indicated
36. nnect the remote fill drain hose as shown in Fig 5 Important When inserting the hose into the fill drain connection item 7 in Fig 5 the connecting device 8 of the hose must lock with an au dible click 21 4 Setting up the instrument 4 4 Setting up the data connections Printer optional e Lift off the instrument console flap 12 e Connect the printer to the printer port 7 via a standard Centronics cable refer Fig 6 Other connections e For additional data transfer and storage the instrument console is equipped with a stan dard 1 44 MB floppy drive 10 for 3 5 dis kettes and with an RS 232 serial port 11 Only instruments which satisfy the re quirements for SELV circuits may be connected to interfaces 7 8 9 and 11 22 Instruction Manual V 1 3 12 2003 4 Setting up the instrument 4 5 Alarm functions Leica ASP300 The Leica ASP300 Is equipped with two different alarm functions Instrument alarm This alarm is generated from within the Leica ASP300 The instrument alarm is generated in all alarm situations Remote alarm This alarm device is installed outside of the Leica ASP300 All alarms all types of alarm are forwarded to the remote dialer con nected to the instrument regardless of whether the local instrument alarm type 1 is connected or disconnected The optional remote alarm system works via a potential free relay If
37. ocessing to allow the retort to vent Do not remove the wax drain hose or the remote fill drain hose until the fill or drain has been entirely completed After refilling replacing the reagent bottles ensure that the lids are tight The bottles must be properly pushed home into the connection manifolds at the rear inner wall of the reagent module Fixatives containing mercuric salts acetic or picric acid will corrode metallic components In the instrument After each paraffin step a retort clean cycle must be run Leica ASP300 9 2 Safety 2 3 Integrated safety devices The instrument is equipped with the following protection features e overpressure protection e protection against excessive vacuum e overcurrent protection e overheating protection Overpressure protection The design of the pressure system ensures that no dangerous levels of pressure can build up Protection against excessive vacuum The vacuum system is not capable of generating a dangerous vacuum condition Overcurrent protection Overcurrent conditions are protected against by both the mains fuse and the separate heating power fuses Overheating protection The design of the heating system ensures that no dangerous overheat ing conditions can occur 10 Instruction manual V 1 3 12 2003 3 Instrument components and specifications 3 1 Specific instrument options Leica ASP300 Reagent Management System RMS The
38. of labels for reagent bottles each set containing 15 labels 1 Splash protector 1 Set of power cords 1 Power cord D 1 Power cord UK ST BU F 5A 13 Reagent bottles plastic included in the instrument Condensate bottle plastic included in the instrument Service kit for pump included in the instrument Drip tray included in the instrument Cover sheet for overflow included in the instrument Console cover mounted to the instrument 3 5 Diskette empty taped to the back of console cover Instruction manual Leica ASP300 multilingual Demo Program CD Rom ssp 0476 34100 0476 35929 0476 34193 0476 34274 0476 34150 0476 34716 0476 34721 0476 35923 0336 35460 0411 34604 0476 35921 0476 34202 0476 35792 0476 34770 0411 13558 0411 27822 0476 34274 0476 34278 0476 35922 0476 34145 0476 34852 0476 34162 0704 37102 14 Instruction manual V 1 3 12 2003 3 4 Technical data Nominal voltage Nominal frequency Main fuses Nominal power Dimensions W x D x H in mm Dry weight unpacked Weight packed Operating temperature range Relative humidity IEC 1010 classification Maximum operating altitude Local remote alarm relays Paraffin wax baths Number of baths Volume liters Melt time Temperature Temperature accuracy Leica ASP300 3 Instrument components and specifications Two factory preset voltage settings not user adjustable 100
39. pervisor access level Press button Installation System Monitor Screen gt gt 2J B A m M ru hiore Favorite 28 Parameter settings Enter instrument name and select lan guage Activate printer optional Enter carbon filter age warning and pump warning threshold Set all parameters as required especially wax bath set temperature Check whether date and time are correct Add the desired reagents and edit the REPLACE REAGENTS warning thresholds Allocate a reagent to each of station Fill the reagent stations bottles either via the ASP300 remote drain fill function SMART SCREEN or manually REAGENT STATUS Create the programs you need Programs can be copied and modified Allocate the most popular programs to the FAVORITES menu and if desired set a fin ish time for these programs and allocate a symbol Instruction manual V 1 3 12 2003 5 Operation 5 1 Reagent handling 5 1 1 Fill drain reagents other than wax 1 Manually changing the reagents e Remove the appropriate reagent bottle from the Leica ASP300 re agent cabinet remove the bottle screw cap X e Drain refill the reagent by pouring to from a bulk container e On the REAGENT STATUS screen verify correct allocation and set the reagent bottle as full 100 Set as Full 2 Use the Remote Fill Drain features of the Leica ASP300 Connect the supplied remote fill drain ho
40. run log will be printed out Important The options selected will be applied to ALL programs All run options can be enabled disabled during the program run if necessary The printer option will be displayed only if a printer has been configured for the instrument Instruction Manual V 1 3 12 2003 5 Operation 5 3 2 Starting a program Fig 31 e Press START to start the program Press OK to confirm the query that follows Enter the number of blocks if the NUMBER OF BLOCKS op tion has been enabled 08 13 irsday e The FINISH TIME button shows the time when the current program Apri will be finished Finish Time e Before a program in progress can be modified the PAUSE symbol must be pressed To modify or delete program steps press EDIT and in the entry win dow carry out any changes you wish to make Press CONTINUE to re start the program Continue The finish time of a program in progress can only be changed as long as the delay step has not yet been completed e Press TABLE to change from graphic to tabular program display press GRAPHICAL to go back to graphic display Leica ASP300 51 5 Operation 5 4 Reagent status as full or empty Reagent Status Blocks Cycles Days Blocks Cycles D Since Since Since Since Since Changed Changed Changed Cleaned Cleaned Cleaned Station Reagent Foarmalin a TS I Thursday 26 April 2001 13 19 51 Sin
41. s com Leica ASP300 These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing pro cesses without prior notice Only in this way Is it possible to continuously improve the tech nology and manufacturing techniques used in our products This document Is protected under copyright laws Any copyrights of this document are re tained by Leica Microsystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photo copy microfiche web cam or other methods including any electronic systems and media requires express prior permission in writing by Leica Microsystems Nussloch GmbH Leica Microsystems Nussloch GmbH Table of contents l Important TOU S sarsma edt 6 1 1 Symos used iMiss Mal Ua laana 6 1 2 Designated USe tesnanar sess shes sctucaavacsasnuapeaueianancactgatdetnasedarvacaaidacbenacdaiaoieiaarateatetcancectantastoenetaraauasinncnte 6 2 ANCE aiaceve size aecvnasast dase ace ava E EO 7 2 1 Sale NnS UC 1 0 og A 1 2 2 WV Al NING Sie ste ceseeushasstcetienueneuntudubeditcespenbunsdiace eTa aE AnA D TERI 8 2 3 integrated safety COVIC OS iia vninsissidecncisetacaetyinisidtstansrdieh a 10 3 Instrument components and specifications cccccssceseseesesesesenessenesesenseeenenseseeas 11 3 1 Specie instrument OP ULOLVS eeo a A 11 3 2 Over
42. se to the remote drain connection refer Fig 11 Important Ensure that the hose is pushed all of the way onto the connection until a clear click is heard e Putthe loose end of the remote fill drain hose into the desired container for the drain fill operation as desired Leica ASP300 29 5 Operation Fig 12 Select station Press key symbol ie ie Station Drain Station Refill 30 Fill drain reagents In FAVORITES press MENU In MENU FUNC TIONS press SMART SCREEN The SMART SCREEN Fig 12 pops up Remote drain e Qn the screen select reagent bottle no 3 and the bulk container Remote The press STATION DRAIN e Check for leaks Remote fill e Qn the screen select reagent bottle no 3 and the bulk container Remote The press STATION REFILL e The remote fill procedure takes less than 3 minutes e Check for leaks Instruction Manual V 1 3 12 2003 5 Operation 5 1 2 Changing the wax Fig 13 Draining the wax e Connect the paraffin drain hose 2 as shown Make sure the hose end is securely kept in in Fig 13 and insert the loose end of the hose the remote container until both the drain has into a remote collecting container been completed and the hose has been purged with pressurized air From the FAVORITES screen press SMART SCREEN see previous chapter e Select the wax bath to be drained and the remote container
43. set up in a well ventilated area free from any ignition sources The room where the instrument is installed is not suitable for people to stay in for longer periods of time unless it is equipped with a fume extraction system Do not operate the instrument in rooms with explosion hazard Moving the instrument The instrument must be installed such that the mains switch at the rear of the instrument item no 5 in Fig 3 can easily be ac cessed at any time After the instrument has been unpacked see unpacking instructions attached to outside of transport crate it must be touched only at the points marked with dots for transport to Its final site of installation Once the instrument has been installed there the brakes on the instru ment rollers must be locked 4 2 Electrical connection e Check the voltage label Fig 2 on the rear of the instrument to ensure that the instrument Is set to the correct voltage range i CAUTION Unit wired for 230 240 Voc Refer changing to qualified service personnel i The power supply voltage for the instrument is factory preset Fig 2 and CANNOT be altered by the user Leica ASP300 19 4 Setting up the instrument Connecting the instrument to mains The instrument MUST be connected to an earthed mains power outlet socket It is recommended that the ASP300 be plugged into a wall socket that has Ground Fault Circuit Interruption GFCI
44. ter port Local alarm connection Remote alarm connection Floppy disk drive Serial port Activated carbon filter Condensate bottle Reagent bottle 13 units Drip tray Wax drain connection Remote drain connection Retort Wax baths Specimen basket Lid for specimen basket Splash protector Leica ASP300 Instrument components and accessories The processor module comprises three paraf fin wax baths and the processing retort Three baskets 21 contain the cassettes Each basket can hold up to 300 cassettes All processing occurs in the stainless steel re tort under the pressure vacuum and tempera ture conditions selected The reagent bottles are stored in the reagent cabinet 13 3 Instrument components and specifications 3 3 Standard delivery packing list Leica ASP300 basic standard delivery 1 Leica ASP300 Basic instrument 1 Accessory kit consisting of 1 Basket hook 0476 34713 3 Specimen baskets assy including lid dividers springs and handle accessories for maximum and optimum usage of basket space 1 Reagent bottle plastic 2 Activated carbon filters assy 1 Remote fill drain hose assy with special connecting device for remote filling draining of processing retort 1 Paraffin drain hose Paraffin wax scraper plastic 1 Lubricant Molykote 111 for valves and O rings 100 g Jumper cable mains 1 Maintenance kit 2 spare lids 9 O rings 1 Stirrer bar assy 2 Sets
45. the threshold altogether e Press OK to confirm The same threshold value applies for all reagent stations con taining the same reagent podo Changing reagent names or reagent groups e al H a ame Bagent Group r t corresponding headline key symbol e n the entry window or via the keyboard enter the new reagent group allocation e Press OK to confirm and save e Reedit all station and programs allocated to the reagent changed 38 Instruction manual V 1 3 12 2003 5 Operation Deleting reagents e Highlight the reagent to be deleted in the SET UP REAGENTS AND WARNING THRESHOLDS screen e Press DELETE e Press OK in the screen prompt Please remember that a reagent which is already used in a pro gram cannot be deleted Adding new reagents to stations Go to SMART FUNCTIONS to remote fill the reagent bottle lf a bottle is filled manually it must be defined as full afterwards For that purpose e Press MENU to access the MENU FUNCTIONS screen e Press REAGENT STATUS e Inthe reagent list highlight the station reagent that has been filled Menu J manually 4 e Press SET AS FULL to mark the station as full Upon activation of the RMS all warning thresholds for the se lected reagent are automatically reset to 0 Leica ASP300 39 5 Operation 5 2 3 Viewing the program list This list Fig 20 displays all programs currently exis
46. ting in the ASP300 You can create e upto 15 processing programs Routina Qveright Program 12 05 ea 3 retort cleaning programs a eee Wax cleaning program High Prionty Very Fast on T AT the processing program names oan can be edited M E Sm new processing programs can be created and existing ones can be deleted Fig 20 Important New processing programs are created by copying existing ones Therefore the list must contain at least one program at all times e Program duration cannot be edited directly e To alter the duration of a program the duration of one or more indi vidual program steps has to be modified Retort and wax cleaning programs are predefined and cannot be altered 40 Instruction manual V 1 3 12 2003 5 Operation 5 2 4 Adding and or modifying programs Creating a new program You must be logged on at supervisor level In the VIEW EDIT PROGRAMS screen Fig 20 highlight a program as similar as possible to the program you wish to create Press COPY to copy the selected program The new program will have the same name as the program copied however the figure 2 will be added Highlight the line containing the new program Press PROGRAM NAME Enter the new program name Editing program steps Press EDIT refer Fig 20 to access the PROGRAM STEPS screen In the headline you will find the PROGRAM NA
47. to 120V 10 or 230 to 240 V 10 50 to 60 Hz 2 fast blowing melting fuses 20 x 5 mm UL listed Order no e 100 to 120 V F 10 A 250 VAC 69411002 e 230 to 240 V F 5 A 250 VAC 69411000 1 000 VA 595 x 680 x 1 325 approx 140 kg 220 kg 15 C to 35 C 10 to 80 non condensing Protective Class 1 Pollution Degree 2 Overvoltage Installation Category Il e 800 V impulse 120 V systems e 1500 V impulse 240 V systems maximum 2 500 m 30 V DC maximum 2 A 2 connections both single voltage free change over contacts can be operated as both normally open and normally closed connections 3 4 3 per bath approx 10 hrs 40 to 65 C 1 C 15 3 Instrument components and specifications Retort Capacity Reagent volume in liters Temperature paraffin Temperature processing reagents Temperature cleaning reagents Temperature accuracy max 300 cassettes 4 3 40 to 65 C ambient or 35 to 55 C 50 to 65 C 1K Filling time approx 90 sec Drain time approx 80 120 140 sec adjustable Impregnation vacuum 70 kPa g Impregnation pressure 35 kPa g Fill vacuum 70 kPa g Drain pressure 35 kPa g General Reagent bottles 10 Cleaning solution bottles 3 Maximum bottle volume 4 3 Printer Optional accessory Pretest check ON OFF Fluid level sensor ON OFF Recirculation pump in out ON OFF a Time before 1st cycle 12 min b Time between cycles 20
48. unctions can be selected 1 Access for service engineers only 2 Access INSTALLATION menu 3 Abort the current program 4 Abort the current program and delete the current allocation of reagents to reagent bottles and retort 5 Delete all programs and reset instrument Status all lists will be empty 6 Store actual instrument status on floppy disk 7 Display run log 8 Restart instrument Fig 10 Leica ASP300 System diagnostics menu lf during initialization Touch here is touched refer Fig 7 and sub sequently the supervisor pass word is entered the SYSTEM DIAGNOSTICS menu Fig 9 opens up giving access to standard in strument settings Attention Serious instrument malfunctions may occur if any settings are modified wrongly Fig 9 To exit this menu the instrument has to be re started press RESTART APPLICATION Press YES to confirm ARE YOU SURE YOU WANT TO refer Fig 10 e Initialization starts again from the screen shown in Fig 7 25 4 Setting up the instrument 4 7 Touchscreen functions 4 7 1 User interface overview Enabled Disabled 26 The Leica ASP300 is programmed and operated via a colored LCD touch screen An on line help feature provides detailed information on all instrument functions HELP can be accessed from any screen by pressing the HELP button Button symbols To activate any functions on the Leica
49. view instrument COMPONENTES ccceccsccsesssssssessesessesseseesesensescessesensesesensaeseesesenseeeans 12 3 3 Slandale delivery Wa CKING NS binsin a A 14 3 4 Technical data scccsavacaaasseaansatancpananiaabsndoncaaeiatnanndaastnaad cscainaaidagoisanachasn panaadcaatantanaaandetnantanenasaaiaenas 15 3 5 Companple redden Sennin a N 18 4 Setting up the instrument ec sseseseseseeeeseseseeeeeeeeaesesesenaeaeseseseeenaeaeseseseneeaeaesesesenenenaeas 19 4 1 IETS EEA e aN AE A EE E EE I EE E 19 4 2 EV EC Lil Gall ONG CG ionnan a a a vaniadiseeueanistan 19 4 3 mstalling TG a CCOSSOMOS ariii a E 21 4 4 Senma UD TE data connections nranskiccemnan ea a anan 22 4 5 Alarm TUNC ONS ernea ta Saat nan a a a N 23 4 6 SWITCHING the INSTFUMENT ON uu cecccccccssssecsesssessececsessesecsessuseesetseseesancessesenseesessesansensasensensas 24 4 1 Touchscreen TUNGUM ana a 26 AV WSECPINECTT ACE OV GE VIG W muson a a N 26 A T 2 ACCESS CONTO ever cescusetices suctwaduataductiecuscusbinae ubbessencutusdasasauatantadnaniutdventercnaidatabueoaiadetgatetenceniae 27 4 8 Check list for first operation at Supervisor access leVEl ccs esessesesessceeeeeeees 28 5 Operatioune Ea ONEEN 29 5 1 Reagen nanaihua a A 29 5 1 1 Fill drain reagents other than Wax arcensiriniinan a 29 SUEZ CHandind te WAK ea ee anieeataaagnanadaaeies 31 5 2 Setting up the instrument parameters ccecccccesssscscsseeessessesesessuceeesenseesessesenseesetsnsensas 34 D
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