GE GM-1 User's Manual
Contents
1. 3 Lid 19 0452 01 B 1 4 Switch 5 Screw 6 Stirrer blade 19 0455 01 A 1 7 Reservoir cylinder 19 0457 01 D 1 8 O ring 19 0364 01 E 11 Base plate 12 Control valve complete 19 0462 01 A 1 13 Knob 14 Screw 15 O ring 16 Foot 19 7320 01 F 4 17 O ring 19 0680 01 E 10 18 Flow control valve FCV 1 19 0049 01 A 1 complete 19 Sealing plug 19 0039 01 E 5 20 Retaining knob 19 0489 01 G 1 21 Stand mount 19 0463 01 H 1 22 Tubing 19 0040 01 10m 23 Stopper 19 0752 01 H 10 i 56 2853 00 AE 2002 12 p3 material of main component Materials Polypropylene Polyvinylchloride Polyamide Fluororubber Polyurethane Superpolyoxymethylene Stainless steel Polyethylene A B C D Polymethylpentene TPX09 E F G H 10 Important user information Meaning Consult the instruction manual to avoid personal injury or damage to the product or other equipment Warranty and liability GE Healthcare Bio Sciences AB guarantees that the product delivered has been thoroughly tested to ensure that it meets Warning its published specifications The warranty included in the i aes conditions of delivery is valid only if the products has been The Warning sign is used to call attention to the necessity to y y P installed and used according to the instructions supplied by follow an instruction in detail to avoid personal injury Be i GE H ealthcare Bio Sciences AB sure not to proceed until the instructions are clearly G2. cleanly at a 45 angle 2 Remove the screw cap and slip it over the tubing 3 Remove the rubber cone with the help of the metal stopper 4 Push the tubing through the rubber cone until it projects about 1 cm 5 Seat the tubing in the nipple Slide the cone along the tubing into the nipple 6 Finger tighten the screw cap GE imagination at work Dismantling the Gradient Mixer The reservoir cylinders 7 control valve 12 and flow control valve 18 may be removed from the base plate by unscrewing them To remove the stirrer blade 6 from the motor unit 1 2 unscrew the nylon screw 5 a few turns It is not necessary to remove the screw completely The lid of the motor unit should not be removed 6 Operation Operating the Gradient Mixer 1 Close the control valve 12 and flow control valve 18 and pour the required amount of starting solution into the mixing reservoir The graduations may be used as an approximate guide to the volume of liquid in the reservoirs 2 Mount the motor unit 1 2 with the stirrer blade 6 on the mixing reservoir 3 Open the control valve 12 slightly so that the channel between the reservoirs fills with solution and a small amount of liquid emerges into the other reservoir Close the control valve 4 Pour the gradient forming solution into the empty reservoir until the liquid levels in the two reservoirs are nearly the same The level in the mixing reservoir should
3. rpm synchronous motor mounted in polypropylene housing A phasing condensor ensures that the direction of stirring is always the same and that the Numbers refer to the enclosed Spare Parts drawing i 56 2853 00 AE 2002 12 pl stirrer blade 6 lifts the liquid entering the mixing reservoir The motor unit is supplied for operation from 100 120 or 220 240 V AC 50 or 60 Hz THE STIRRER BLADE 6 is secured to the motor axle by a nylon screw 5 It is unnecessary to remove the screw to release the stirrer blade THE CONTROL VALVE 12 regulates the flow of liquid between the two reservoirs It is fully open when screwed out one complete revolution from the closed position THE FLOW CONTROL VALVE 18 is a needle valve designed for shut off as well as flow control The valve nipple accepts capillary tubing 1 6 1 9 mm o d Eluent flow is regulated with the large knob A The line on this knob and the markings on the valve housing can be used as a flow rate guide The valve can thus be opened to a previously determined position to obtain a given flow rate To obtain reproducible flow rates the valve should always be returned to the shut position before setting THE STAND MOUNT 21 may be used to support the Gradient Mixer on a laboratory stand When not required it may be removed from the base plate 11 by unscrewing the retaining knob 20 5 Installation Connection of tubing to the flow control valve 1 Cut the tubing
4. 1 051 g 56 2853 00 AE 2002 12 p4 GE imagination at work
5. 27302 212 Japan Tel 81 3 5331 9336 Fax 81 3 5331 9370 Latin America Tel 55 11 3933 7300 Fax 55 11 3933 7315 Middle East and Africa Tel 30 2 10 96 00 687 Fax 30 2 10 96 00 693 Netherlands Tel 0165 580 410 Fax 0165 580 401 Norway Tel 2318 5800 Fax 2318 6800 Portugal Tel 21 417 7035 Fax 21 417 3184 Russia amp other C I S amp N I S Tel 7 095 232 0250 956 1137 Fax 7 095 230 6377 South East Asia Tel 60 3 8024 2080 Fax 60 3 8024 2090 Spain Tel 93 594 49 50 Fax 93 594 49 55 Sweden Tel 46 18 612 19 00 Fax 46 18 612 19 10 Switzerland Tel 01 802 81 50 Fax 01 802 81 51 UK Tel 0800 616 928 Fax 0800 616 927 USA Tel 1 800 526 3593 Fax 1 877 295 8102 GE Healthcare are trademarks of General Electric Company GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden GE Healthcare Bio Sciences Ltd GE Healthcare Place Little Chalfont Buckinghamshire HP7 9NA England GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 USA GE Healthcare Europe GmbH Munzinger Strasse 9 D 79111 Freiburg Germany GE Healthcare Bio Sciences KK Sanken Building 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan All goods and services are sold subject to the terms and conditions of sale of the company within the General Electric Company group that supplies them A copy of these terms and conditions is available on request Produced by Wikstr ms Sweden 1021611 12 2002 Printed matter Licence 34
6. Bi uctions e Gradient Mixer GM 1 1 Introduction The Gradient Mixer GM 1 120 V Code No 19 0495 01 230 V Code No 19 0485 01 consists of two identical reservoirs mounted on a base plate connection between the reservoirs is made via a valve Mounted above the mixing reservoir is a motor unit which drives a stirrer blade The outlet from the mixing reservoir to the column is fitted with a flow control valve FCV 1 The Gradient Mixer is conveniently supported on a laboratory stand Capillary tubing 2 m is also supplied 2 Safety Warning When using hazardous chemicals all suitable protective measures such as protective glasses must be taken 3 Unpacking Unpack the equipment carefully and check the contents of the carton against the packing list 4 Description THE BASE PLATE 11 is a precision machined polypropylene block on which the reservoir cylinders 7 are mounted Sealing between the base plate and the cylinders is provided by O rings 8 THE RESERVOIR CYLINDERS 7 are straight polymethylpentene tubes threaded at one end and with air inlets at the other end Each cylinder has a capacity of 300 ml and is graduated to 250 ml at 25 ml intervals to give a rapid check of the volume of liquid remaining in the reservoirs The mixing reservoir carries the motor unit 1 2 and the other reservoir is fitted with a snap on lid 3 THE MOTOR UNIT 1 2 consists of a fully encapsulated low speed 250 or 300
7. E Healthcare Bio Sciences AB shall in no event be liable for incidental or consequential damages including without understood and all stated conditions are met Caution boas i limitations lost profits loss of income loss of business The Caution sign is used to call attention to instructions or E opportunities loss of use and other related exposures conditions that shall be followed to avoid damage to the PP gt P 7 however caused arising from the faulty and incorrect use of product or other equipment Be sure not to proceed until the the product instructions are clearly understood and all stated conditions are met Note The Note is used to indicate information important for trouble free or optimal use of the product Should you have any comments on this instruction we will be pleased to receive them at GE Healthcare Bio Sciences AB S 751 82 Uppsala Sweden to order Asia Pacific Tel 852 2811 8693 Fax 852 2811 5251 Australasia Tel 61 2 9899 0999 Fax 61 2 9899 7511 Austria Tel 01 576 0616 20 Fax 01 576 0616 27 Belgium Tel 0800 73 888 Fax 03 272 1637 Canada Tel 1 800 463 5800 Fax 1 800 567 1008 Central East South East Europe Tel 43 1 982 3826 Fax 43 1 985 8327 Denmark Tel 45 16 2400 Fax 45 16 2424 Finland amp Baltics Tel 358 0 9 512 3940 Fax 358 0 9 512 1710 France Tel 0169 35 67 00 Fax 0169 41 9677 Germany Tel 0761 4903 401 Fax 0761 4903 405 Italy Tel 02 27322 1 Fax 02
8. be slightly higher than the level in the other reservoir to avoid a step at the beginning of the elution 5 Open the control valve 12 fully one complete revolution start the motor and begin the elution by opening the flow control valve 18 If a pump is used to control the eluent flow the flow control valve 18 should be fully open one complete revolution The Gradient Mixer will give linear ionic strength gradients and reproducible but non linear pH gradients The exact shape of the pH gradient obtained will depend on the buffer salts used and the ionic strengths and pH s of the buffer solutions The use of gradient elution in ion exchange chromatography is described in the booklet Ion Exchange Chromatography principles and methods 7 Maintenance Warning When using hazardous chemicals all suitable protective measures such as protective glasses must be taken Cleaning Ordinary laboratory detergents may be used to clean the gradient mixer Enzyme detergents are recommended for removing protein aceous contaminants The mixer including i 56 2853 00 AE 2002 12 e p2 the stirrer blade but not the motor unit may be autoclaved at 121 C for 20 min Caution Do not autoclave the motor unit or drain the motor in strong detergents 8 Technical specification Mixing chamber 300 ml volume Environment 4 40 C 20 95 relative humidity Material of wetter parts Polymethylpentene Polypropylen
9. e glass fibre reinforced polypropylene and fluororubber Chemical resistans The wetted parts are resistant to aqueous and organic solvents commonly used in ion change chromatography and solutions containing inorganic and organic acids alkalis and alcohols 100 120 VAC or 220 240 VAC 50 60 Hz Power requirements Power consumption 7VA Safety standards This products meets the requirements of the Low Voltage Directive LVD 73 23 EEC through the harmonized standards EN61010 1 and EN60742 Note The declaration of conformity is valid for the instrument when it is e used in laboratory locations e used in the same state as it was delivered from GE H ealthcare Bio Sciences AB except for alterations described in the user manual e used asa stand alone unit or connected to other CE labelled GE H ealthcare Bio Sciences AB instruments or other products as recommended 9 Spare parts Caution Only spare parts approved or supplied by GE Healthcare Bio Sciences AB may be used for maintaining and servicing of Gradient Mixer GM 1 The figure indicates the correct locations of the different parts of the Gradient Mixer For replacements please order according to the spare part list below using the appropriate code number Detail No Designation Code No Material No per pack 1 Motor unit complete with 18 1121 66 A 1 detail Nos 3 4 5 110V 2 Motor unit complete 230 V 18 1121 67 A 1
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