Home

UltiMate 3000 AFC-3000 Automated Fraction Collector

Contents

1. Drain Close the diverter valve prior to a run to avoid accidental spillages AFC CollectMode vessels a Sequence AFC MovementMode SawVertical Define how the drop former travels over the collection Do only define once and do not change during a run or Interrupt Define how the diverter valve behaves between tube changes AFC TubePosition FractionCollection TubePosition Synchronize the fraction collector position with Operating Instructions Page T1 UltiMate 3000 Series AFC 3000 Automated Fraction Collector the current position of the fraction collection driver prior to a run PRK KKK kc ck ck KK ke kc KK ko ke ko kk ke ck ck A KAZ KAZ KA KK ke ke kk ek kc ke kc ke ck kc c kc kc kc kc kk EEEE Definition of triggers for fraction collection starts here PRK kk kk koc AK AZ kc kc KK KK khe koc kk ke ck ec ke A KAZ kc kc ke kc KKK ke ko ke ke ke kc kc ke ck kc kc kc kc kc kc kk e KK Trigger FracStartDetected AFC Valve Log Log Fractioncollection EndTrigger Trigger FracTubeChange AFC Valve AFC TubePosition FractionCollection AFC Valve Log Log FractionCollectiorn EndTrigger Trigger FracEndDetected AFC Valve AFC TubePosition FractionCollection Log Log Fractioncol EndTrigger lection TubePosition TubePosition TubePosition TubePosition TubePo
2. ccccccsseceeeceesseeeeees 71 Tay eoe eo me tee 20 30 69 Troubleshooting u 59 Communication Problems 59 MESSAB CS ire intended 60 KR E 59 MUD ES ec detta aei We REI GTI de 20 U Unit Description een 13 Configurations esses 15 OVerVie WA esee tu ROI EIS 13 Principle of Operation ee 14 Universal Drip Tra 20 30 69 et TEE 24 EE 19 36 User Information eese 11 V Verify Installation 43 W Weekly Cleaning z eee eee 63 Page 82 Operating Instructions
3. If you detect a leak or other damage to a fraction collector component you must replace it Operating Instructions Page 63 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 7 4 Installing the Kit for Normal Phase LC Description Part No Kit for normal phase LC including 6702 0400 1 0 mm ID stainless steel drop former and grounding cable D Warning Do not perform normal phase applications without a proper grounding protection of the drop former Non conductive non polar normal phase eluents for example hexane can lead to a build up of electrostatic charges on an ungrounded drop former The electro static discharge can create a fire and or explosion hazard D Avertissement N effectuez pas d applications en phase normale sans vous tre assur que votre faiseur des gouttes est proprement isol e et mise la terre Les solvants de la phase normale non conducteurs et non polaires par exemple hexane peuvent entra ner une accumulation de charges lectrostatiques sur le faiseur des gouttes en l absence de mise la terre Le d charge lectrostatique peut repr senter un risque d incendie et ou d explosion 1 Switch the valve to Drain position then shut down and unplug the fraction collector Set the pump flow to 0 2 Carefully move the diverter valve assembly along the Y arm to the front 3 Remove the current drop former Then screw the SST drop former finger tight into the diverter valve 4 Pu
4. For instructions on shutting down the fraction collector see section 5 3 page 57 7 2 Cleaning the Fraction Collector Cleaning the AFC 3000 is the primary maintenance task you perform Failure to do so regularly causes increased wear and reduces the fraction collector s life You must clean the AFC 3000 both daily and weekly to prevent damage and extend its life It is especially important to clean up spills and remove contaminants such as abrasives from the fraction collector s moving parts It may also be necessary to chemically neutralize spills The following sections explain daily and weekly cleaning procedures 7 2 1 Daily External Cleaning Use of the AFC 3000 often results in spills on fraction collector components such as the fraction tray Good maintenance requires that you clean the fraction collector daily To do so complete the following steps 1 Shut down and unplug the fraction collector i Tip For information about shutting down the fraction collector see section 5 3 page 57 Page 62 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 Wipe the fraction tray fraction collector cabinet and fraction collector arm using a towel dampened with a lab grade cleaning agent A Important Do not allow the cleaning agent to come into contact with the lead screws Never lubricate either of the two lead screws A Important Assurez vous que les broches filet es ne soi
5. If the Demo Mode is enabled the Port list will be unavailable on the AFC page Page 40 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector AFC Page This page is used to configure the connection between Chromeleon and the fraction collector Dionex AFC 3000 Fraction Collector Dionex AFC 3000 Fraction Collector AFC Error Levels Device Name AFC Serial Communication Port feta Sh Y Baudrate 9600 Parity None Data Bits 8 Stop Bits 1 Handshake Xon Xoff Fig 15 AFC page e Device Name The name used to identify the fraction collector in the installation environment and in the Chromeleon client program To control the fraction collector with the existing control panels accept the default name If you enter a different name you may have to re link the controls on the control panels and edit the device name in the program files e Serial Communication In the Port list select the serial interface port to which the fraction collector is connected This is either the COM port on the Chromeleon computer to which the RS 232 serial cable is connected or if a USB cable is used for the connection the virtual COM port USB Seria Port that was created during installation of the USB drivers Windows automatically allocates a number for the virtual COM port for example COMS usually this is the COM port with the highest number In addition the current interface parameters are dis
6. page 40 Chromeleon Cont d The USB port on the computer is Check the USB port on the computer not ready for operation 6 3 Messages in the Chromeleon Audit Trail When the fraction collector is operated with Chromeleon messages are displayed in the Chromeleon Audit Trail The following table lists fraction collector related messages along with appropriate remedial actions In addition to the messages in the table other messages may appear In this case note the exact wording of the message and contact Thermo Fisher Scientific Service for Dionex HPLC Products if you are unable to eliminate the problem Can t open communication channel Check the configuration for the correct COM port selection Serial Device Driven COM2 section 3 5 2 page 40 Command for function Connect The AFC 3000 cannot connect to Chromeleon See failed Communication Problems page 59 Command for function Rack Verify that you have configured the racks in the appropriate failed order and that you have not configured more racks than can be installed page 49 Command to change property You have entered a tube position that exceeds the number of TubePosition failed positions on the installed racks Verify that you have defined a valid rack type Enter a valid tube position Device is not remote The AFC 3000 is not connected to Chromeleon Execute the Connect command Error P0040 Value out of range Verify that you have entered value
7. 2 Operating Principle The AFC 3000 Automated Fraction Collector collects fractions from the UltiMate 3000 or any other HPLC system A solenoid diverter valve directs the flow either to the waste or to the fraction collection tubes When Chromeleon sends a trigger signal to the AFC 3000 calling for fractions to be collected the diverter valve moves over the assigned collection tube and is then switched to route eluent flow into the tube During any movements all tubes remain stationary while the moving diverter valve assembly positions the drop former over the appropriate tube or above the rinse station The fraction collector includes the following fluidic components Sample inlet capillary Eluent is supplied from the detector outlet to the common inlet port of the diverter valve through this tubing Diverter valve This valve directs the eluent from the detector to either waste or to a collection tube Drop former This component is attached to the diverter valve and forms droplets or a jet of eluent for collection into tubes Waste tubing This tubing connects the waste outlet port of the diverter valve to a waste container or to a drainage system Rinse station A tube like shaped port which is mounted to the head of the fraction collector The outlet of this station is connected to a waste container or a drainage system through a tubing Collection tube The tube into which eluent sample is collected The fraction collector includ
8. Chromeleon as described in section 3 5 page 39 Before you can operate the fraction collector with Chromeleon you have to connect the timebase in which the fraction collector is installed to the Chromeleon client program section 5 2 1 Two modes of software control are available e Direct control with the parameters and commands in the Commands dialog box F8 box or on a control panel page 46 e Automated control with a control program PGM gt page 52 Operating Instructions Page 45 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 2 1 Connecting to Chromeleon Start the Chromeleon Server Monitor if it is not already running page 39 Click Start to start the server Start the Chromeleon client by clicking the Chromeleon icon GH on the desktop If the Chromeleon icon is not on the desktop click Start on the taskbar point to Programs or All Programs depending on the operating system point to Chromeleon and then click Chromeleon Connect the Chromeleon client to the timebase in which the fraction collector is installed For details about how to do this from the Commands dialog box see page 46 For details about how to do this on a control panel see page 47 Before turning off the fraction collector by the main power switch always disconnect the module in Chromeleon Disconnect command 5 2 2 Direct Control With direct control you select operating parameters and command
9. Instructions Page 71 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 72 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 9 Accessories Spare Parts and Consumables Accessories spare parts and consumables for the fraction collector are always maintained at the latest technical standard Therefore part numbers are subject to alteration However updated parts will always be compatible with the parts they replace Because the life span of the fraction tubes varies you should maintain an adequate supply of spare tubes When you need to purchase additional supplies it is extremely important that you choose the appropriate sizes and materials When you purchase fraction tubes make sure they meet the following requirements e The diameter ofthe fraction tube matches the rack size you are using e The height does not exceed 150 mm e The material is compatible with the samples and solvents you are using A Important Do not use mismatching fraction tubes Be sure your tubes meet the given requirements A Important N utilisez pas de tubes de fraction incompatible Assurez vous que vos tubes r pondent aux exigences donn es 9 1 Standard Accessories included in the shipment The following accessories are shipped with the fraction collector The list is subject to change without notice The part number always refers to the packing unit For more information contact the
10. Q3 Reset Connected Q4 Disconnect ga Connect Fig 16 Commands dialog box 6 Verify that the fraction collector is connected to Chromeleon If it is not connected execute the Connect command In addition to the fraction collector commands and parameters the Commands dialog box provides access to all commands and parameters available for all devices that are installed in the selected timebase To open the AFC 3000 control panel 1 Open the Commands dialog box and connect the AFC 3000 to Chromeleon page 46 2 Onthe View menu click Default Panel Tabset or click the corresponding icon on the toolbar EE and then connect to the Chromeleon server Chromeleon creates centralized control panels called panel tabsets for all timebases available on the Chromeleon server A panel tabset provides control panels for the individual instruments in a timbase and in addition one or more panels for performing system wide functions for example creating and running sequences For more information about panel tabsets see the Chromeleon Help 3 On the Panel Tabset for your timebase click the AFC 3000 tab page Fig 17 Operating Instructions Page 47 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Chromeleon Panel Tabset1 FI File Edit View Workspace Qualification Control Batch Window Help PC1UltiMate3000RS 77 gt f Home Sequence Control DGP 3600 RS SD Sampler Col Comp DAD Comma
11. Thermo Fisher Scientific sales organization for Dionex HPLC Products Description Part No Quantity in the accessories kit AFC 3000 accessories kit including Mee 24 V external power supply 6702 9010 Diverter valve assembly including screws for installation and 1 mm ID PEEK drop former Fluidics kit incl sample inlet tubing 1 0 mm x 4 m and waste tubing 1 0 mm x 1 8 m with nuts and ferrules Drain tubing 2 x 1 8 m Included in 6040 0005 2 Elbow Y and T pieces for drain connections Included in 6040 0005 7 Polypropylene tubes 8 mL OD 13 mm ee d 180 USB cable type A to type B USB 2 0 5 m Spiral tube Rack 90 position 8 mL 13 mm x 100 mm 6702 0090 Operating Instructions Page 73 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 9 2 Optional Accessories Kit for low flow rates 6702 0300 Kit for flow rates up to 10 mL min Includes different diverter valve assembly drop former and tubing Kit for normal phase LC 6702 0400 SST drop former for use with normal phase solvents Provides grounding via a cable Drain kit for UltiMate 3000 systems 6040 0005 The kit includes all required components for system drainage Fraction Collection License 5960 0038 Additional Chromeleon license required for basic fraction collection page 21 Extended Fraction Collection License 5960 0039 Additional Chromeleon license required for advanced fraction collection page 21 This licen
12. collector components that wear out under normal use and purchase the appropriate supplies for use with the fraction collector A Important Damage or malfunction that results from unsatisfactory operating conditions may constitute misuse or abuse and may therefore be excluded from warranty coverage A Important Dommage ou de dysfonctionnement qui r sulte de conditions d exploitation insatisfaisantes peut constituer une utilisation abusive ou l abus et tre exclu de garantie The installation site must meet the following requirements e The main power switch and the main power receptacle are on the rear panel Make sure that Free and unrestricted access to the main power switch 1s ensured at all times The power cord of the device can be easily reached and disconnected from the power line at all times Provide sufficient space behind the device to unplug the cable e Make sure that the installation site meets the power and environmental specifications listed in the Technical Information section page 71 e Install the instrument in the laboratory on a stable surface that is free of vibrations e Avoid rough handling of the AFC 3000 Do not expose the fraction collector to vibration or shock e Make sure that the surface is resistant to solvents e Avoid locations with extreme changes in temperature for example caused by direct sunlight or drafts and high humidity e Allow sufficient clearance behind and on the side
13. completely gone Operating Instructions Page 25 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 3 Installing the Fraction Collector The AFC 3000 is designed for easy installation consisting of two parts assembling the fraction collector and connecting it to the analytical instrument For the most part you can install the AFC 3000 without using tools In fact using tools such as screwdrivers or pliers to perform most installation tasks may result in a damaged or unusable instrument You can remove the knurled screws with tools if necessary but do not tighten them with anything other than your fingers To install the fraction collector you must complete the following tasks each of these tasks will be discussed in detail later in this section e Mount the diverter valve assembly page 27 e Establish connections on the diverter valve page 28 e Attach the rinse station page 29 e Connect the rinse station tubing page 29 e Check the proper alignment of the universal drip tray e Place the fraction tube racks on the tray page 30 e Setthe drop former height adjustment page 33 e Establish external connections page 35 e Setup the fraction collector in Chromeleon page 39 A Important Ensure that the power is off before proceeding with installation A Important Assurez vous que l alimentation secteur est teint avant de proc der l installation Page 26 Ope
14. en contact prolong avec du chloroforme CHCI3 du dim thyle sulfoxide DMSO ou du tetrahydrofuran THF De plus il est attaqu par des acides concentr s tels que l acide sulfurique et l acide nitrique ou d un compos du hexane thyle ac tate et m thanol Ceci peut causer des capillaires de fuite ou risquer des capillaires d clater Ces acides peuvent cependant tre utilis s dans le cadre de proc dures de nettoyage condition que l exposition soit br ve N utilisez pas de capillaires cras s pli s abim s ou endommag s Les connecteurs capillaires pour pourrait tre contamin par des substances dangereuses ou des substances dangereuses pourrait sortir des connexions ouvertes Portez des lunettes de protection lorsque vous manipulez des capillaires en silice fondue pendant l installation d coupe etc e Quand les capots de protection de l appareil sont d mont s vous tes expos s des connexions lectriques sous haute tension deviennent accessibles D branchez l instrument de toute source d alimentation lectrique avant de retirer les capots Ne d montez les capots de protection que si cela est explicitement demand au cours de ces instructions Les capots de protection devraient tre d mont s uniquement par le personnel de service de Thermo Fisher Scientific e Remplacez les c bles de communication d fectueux e Remplacez les cordons d alimentation lectrique d fectueux Utilisez unique
15. phases for example methanol that are commonly used in reversed phase RP liquid chromatography For normal phase NP applications observe the safety instructions for the fraction collector on page 4 For the safety instructions in French see page 7 e The fraction collector may be operated only using the components originally supplied with the unit and within the technical specifications and environmental conditions page 71 that are outlined in this manual e Use only standard solvents HPLC grade preferably LC MS grade 0 2 um filtered and buffers compatible with the flow path materials Note any special properties of the solvents such as viscosity boiling point UV absorption UV VIS detector refractive index refractive index detector and dissolved gas degasser If there is any question regarding appropriate usage contact Thermo Fisher Scientific before proceeding D Warning If the device is used in a manner not specified by Thermo Fisher Scientific the protection provided by the device could be impaired Thermo Fisher Scientific assumes no responsibility and will not be liable for operator injury and or instrument damage Whenever it is likely that the protection is impaired the instrument must be disconnected from all power sources and be secured against any intended operation Page 10 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector D Avertissement Si l instrument e
16. reached While the valve is switched to drain and the drop former moves to the next tube the delivered sample will be directed to the waste Continue The diverter valve maintains the previously defined valve state during and after any diverter valve movement In this mode either all sample goes to the waste or all sample goes through the drop former This mode is designed to be used with the funnel rack A Important To avoid accidental contamination of empty tubes never use Continue movement mode in combination with SawVertical collect mode A Important Afin d viter contamination des tubes n utilisez pas le mode de mouvement Continue avec le mode de collection SawVertical 4 Click Execute Operating Instructions Page 51 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 2 6 Automated Fraction Collection with Chromeleon The Fraction Collection driver in Chromeleon facilitates the process of collecting fractions Before you start verify that this driver is installed in the same timebase as the AFC 3000 page 40 With automated control you create a program file PGM for automated operation of the fraction collector The Program Wizard assists you in creating a program for fraction collection control On the File menu select New and then select Program File The wizard guides you through program creation On each wizard page make the desired settings or accept the default values For additional infor
17. the Kacktonbteurabion seu ste seasacsuchecestessvsavoncbatnsarsasvecented 49 5 24 Defining the Collect Mode biu derer ber ita t e ta i kubus 50 52 0 Defining the Movement Mode oerte tarea ete e sd e aie 5 5 2 6 Automated Fraction Collection with Chromeleon ee 52 5 3 Shutting Down the Fraction Collector sac cain eet unes e ert eene 57 5 4 Flushing the Rinse Station and Eluent Flow Path 57 5 5 Routine and Preventive Malntenatiee i eim era pte tae iaa t eda red A ed tan 58 NEM RID ICI E 59 6l Ee ee eege 59 6 2 Power and Communication Problems 1 i reete dte trt annee reae eene 59 6 3 Messages in the Chromeleon Audit Trail sse 60 To SBE VIGO sn sn 61 7 1 General Notes and Safety Precautions s edo eu tenda i Qe posed nan 61 7 2 Cleaning the Fraction Collector 4332503 math th o asd dert da 62 Tal Daily External Clean sonne annees ec iib abu iatis 62 2 27 Weekly E e oso cai eiu Oo DA de o E ud 63 13 Checking Tor E ues Eten qve bte oce aid dei 63 7 4 Installing the Kit for Notmal Phase LC aee ee eot en eee ert eS 64 7 5 Installing the Kit Tor Low Flow Rates 2e ee a nee ss ee 66 7 6 Replacing the Diverter Valves sido ee nd Sad er ues rie Re tds 67 7 7 Replacing the Universal Drip tay ee pti ea tr IR Coda uei qr Ub Pase a fes 69 1 8 Updating the e 69 8 Technical Information uei se gegeckst Eed deca Una epa d
18. the drop former the device will turn Off after ca 20 seconds to avoid damage 5 Turn the fraction collector off and manually move the diverter valve assembly to the four corner positions of the installed fraction racks This is to verify that the adjusted height is suitable for all positions despite any tolerances that may exist Page 34 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 4 Connecting the Fraction Collector The next steps in the installation process involve connecting the AFC 3000 to the power source and to the Chromeleon computer The following sections explain how to establish these connections 3 4 1 Connecting the External Desktop Power Supply A voltage specific external desktop power supply is delivered with each AFC 3000 Use the external desktop power supply shipped with the fraction collector to connect the instrument to the main power source 1 Place the AFC 3000 within 1 2 meters of a power outlet 2 Connect the external desktop power supply to the DC power input connector on the rear panel of the fraction collector Fig 3 page 19 3 Connect the supply side of the power supply to the power source that is connected to a true ground The input rating is AC 100V 240V 1 9 A with an output of DC 24V maximum 3 33 A No manual adjustment is required to adapt the line voltage to local voltage requirements D Warning Never use a power cord other than the pow
19. the screw in the diverter valve bottom side Use the screwdriver tool shipped with the kit to carefully tighten the screw You may have to retighten the screw if you observe leakage during operation x Fig 27 Installing the low flow drop former 6 Mount the diverter valve to the diverter valve assembly by using the hexagon wrench shipped with the kit Fig 28 page 67 The diverter valve must be mounted with the waste connection facing upward 7 Tighten the finger tight knurled screw that attaches the rear cable to the mounting block Fig 30 page 68 8 Tighten the finger tight knurled screw that attaches the rear cable to the diverter valve and connect the grounding cable from the rear cable to the ground cable screw and tighten the screw Fig 29 page 68 Page 66 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 9 Install the diverter valve connections section 3 3 2 page 28 A low flow inlet capillary is shipped with the kit 10 Verify that the alignment is still correct page 44 i Tips After installing the low flow inlet capillary always determine the delay time or volume Note that the carry over volume of the kit for low flow rates is smaller than with the standard configuration Exclusively use the low flow drop former within the specified flow rates up to 10 mL min At higher flow rates the Jet from the drop former may cause sprays or spill
20. valve assembly Assembly to which the diverter valve is attached The mounting height of the diverter valve assembly is manually adjustable to attain the proper clearance above the collection tubes and rinse station 4 X travel slot Front cover opening which facilitates travel of the fraction collector arm in the right to left or X direction see also section 2 2 page 14 5 Rinse station The tube shaped port mounted to the cabinet of the fraction collector where eluent may be dispensed and delivered to the waste The outlet of the rinse station is connected to a waste container or drainage system 6 Universal drip tray with Drip tray which allows mounting of supported rack types The tray drain guides spilled solvents into a waste container Operating Instructions Page 17 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 5 Rear Panel Devi EN 110905AF C3800 Pom en LS SIE AUXILIARY Fig 2 Rear panel view No Description 1 Type label 2 Auxiliary I O connection Connects to diverter valve assembly to provide power to the diverter valve and grounding to the stainless steel drop former if equipped Power switch Main power receptacle Serial communication port unused USB port Universal Serial Bus for connection to the Chromeleon computer COM 1 RS 232 port serial for connection to the Chromeleon computer OOM O
21. 2 0100 is required The vials should have an opening as large as possible for unerring fractionation 22 vials WPS 3000 4 mL 15 mm OD 6820 4084 In addition a rack adapter part no 6702 0100 is required 10 vials WPS 3000 10 mL 22 mm OD 6820 4086 In addition a rack adapter part no 6702 0100 is required 4 well plates Four 96 well plates 6702 0200 In addition up to six 250 mL Schott bottles part no 2270 0026 can be placed directly on the universal drip tray To ensure correct installation of the Foxy racks a set of positioning pins is available from Thermo Fisher Scientific part no 6702 9006 For details on rack installation and allowed combinations of fraction formats see section 3 3 5 page 30 Page 20 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 7 Chromeleon Software The fraction collector can exclusively be controlled by the Chromeleon Chromatography Management System For operating the fraction collector with Chromeleon the following licenses are required Timebase Class 1 Fraction Collection part no 5960 0038 for basic fraction collection with wizard based program setup and fraction reporting Extended Fraction Collection part no 5960 0039 for advanced fraction collection The Extended Fraction Collection license includes the Fraction Collection license In addition it supports functions for advanced fraction collection such as au
22. 2 0300 Diverter valve replacement for standard diverter valve Funnel rack 6702 1021 Rack 10 position WPS 10 mL 22 mm OD 6820 4086 Rack 22 position WPS 4 mL 15 mm OD 6820 4084 Operating Instructions Page 75 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 76 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 10 Appendix Chromeleon Example Program Ne A utomated Fraction Col HPG 3x00 WPS 3000SL VWD 3x00 TempCtrl Temperature Nominal Temperature LowerLimit Temperature UpperLimit ReadyTempDelta Pressure LowerLimit Pressure UpperLimit MaximumFlowRampDown MaximumFlowRampUp A Equate B Equate DrawSpeed DrawDelay DispSpeed DispenseDelay VasteSpeed SampleHeight InjectWash LoopWashFactor PunctureOffset Sampler PumpDevice InjectMode SyncWithPump Pump Pressure Step Example program for fraction collection with the AFC 3000 Pump Pressure Averag Data Collection Rate TimeConstant AFC Valve lector and the following instruments On 20 0 C 4 0 C 45 0 C None 0 bar 150 bar 30 000 ml min 30 000 ml min W SA W SB ud 20 000 ul s 3000 ms 2 000 u1 s 2000 ms 4 000 ul s 1 000 mm NoWash 0 000 0 0 mm W Pump W Normal Off Auto On 25 0 Hz 0 06 s
23. 30th 2013 Operating Instructions Page I UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page II Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector Table of Contents MB inci uri fr 1 IVA Howto USE This Mania eo teta Le ale oe v oie ooo ooo l 1227 Safety formation eene eo eternal da red oeste i don dd 2 1 2 1 Symbols on the Fraction Collector and in the Manual 2 IESSE 3 1 2 3 ASONMSISNES e EE 6 EE G RE 10 1 4 Federal Communications Commission FCC Note 11 D gt CVV CR VICW ee 13 2A Ben dE EE 13 22 Operating Prmetple ues a oe nals pa de ea od rente esso tasca eam Step 14 2 3 Fraction Collector Configurations x c ccccisscccctecciesssvateosstsceseccsapnisne de ccbeasvvndeodatasecesn s 15 2 4 Front Panel GE 17 2 29 c Rear PANNES P O O ades T t aed uaa ast dieu me Medi ed 18 2 5 PONS Is dece tite csi s tac voa teretes Seed de aduer i ttes 19 2 53 USB POR es sis uec eaaet oet Scd ate cael t Led oui icta ste 19 275 3 TRS 292 Seri l E EE 19 2 5 4 Auxiliary O Connection Sees Sea Sete Sere ER e e duse de e eile a 19 2 6 Universal Fraction Tray Racks and Tubes 20 2 41 Chromieleon SOfDWAEe EE 2 EM rri sn s h 23 St Facility Komerode deed 23 Did UNpaACKIN EE 24 3 3 Installing the Fraction Collector uie Ie reete teer aree dee 26 3 3 1 Mounting the Diverter Valve Assembly eee een 27 3 3 2 Diverter Va
24. UHPLC Thermo focused SCIENTIFIC Thermo Scientific Dionex UltiMate 3000 Series Automated Fraction Collector AFC 3000 Operating Instructions Original Operating Instructions Revision 1 2 Date October 2013 2013 Thermo Fisher Scientific Doc No 4820 7250 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector CE Declaration of Conformity Original Declaration Product Thermo Scientific Dionex UltiMate 3000 Fraction Collector Type AFC 3000 Dionex Softron GmbH herewith declares conformity of the above products with the respective requirements of the following regulations e EMC Directive 2004 108 EC e Low Voltage Directive 2006 95 EC The electrical safety of the products was evaluated based on the following standard e DIN EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General Requirements The Electromagnetic Compatibility EMC of the products was evaluated based on the following standard e DIN EN 61326 2006 Electrical equipment for measurement control and laboratory use EMC Requirements This declaration 1s issued for the manufacturer Dionex Softron GmbH Part of Thermo Fisher Scientific Inc DornierstraBe 4 D 82110 Germering by the Managing Director R diger Obst and the Vice President HPLC Fraser McLeod August
25. a cud eS ess 71 9 Accessories Spare Parts and Consumables e eeee eee eerte ee eren eee en ne ennu 73 9 1 Standard Accessories included in the shipment eee 73 22 he KEREN 74 9 3 Consumables and Spare Parts hrs tas et teens p Rede te ed een ee nn nier 75 10 Appendix Chromeleon Example Program 77 iMi CR c 81 Page ii Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 1 Introduction 1 1 How to Use This Manual The layout of this manual is designed to provide quick reference to the sections of interest to the reader However in order to obtain a full understanding of the fraction collector Thermo Fisher Scientific recommends that you review the manual thoroughly before operating the fraction collector All descriptions in the manual apply to the AFC 3000 Automated Fraction Collector Therefore the term the fraction collector or the AFC 3000 is used throughout the manual Notes The device configuration may vary for example the fraction collector may be equipped with differing rack configurations therefore not all descriptions necessarily apply to your particular instrument If some detail applies to only one fraction collector model or version that model version is identified by name It may happen that the representation of a component in this manual is different from the real component However this does not influe
26. a saw shaped path The movement matches the imprinted numbering on all rack types that are available from Thermo Fisher Scientific J 36 2 x MK JOE VC M AC SS NL e N dg N YI ATE SAS e lt K CX X Ewe Je 7 WEN AC 7 eO 4 sf LH SawVertical Vertical Fig 19 Vertical and SawVertical collect modes A Important To avoid accidental contamination of empty tubes never use SawVertical collect mode in combination with Continue movement mode A Important Afin d viter contamination des tubes n utilisez pas le mode de collection SawVertical avec le mode de mouvement Continue 4 Click Execute i Tip Schott bottles will always be processed in SawVertical collect mode The collect mode setting 1s not supported with Schott bottles Page 50 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 2 5 Defining the Movement Mode Define the diverter valve behavior during any drop former movements l 2 Open the Commands dialog box page 46 Click the plus sign beside AFC Click MovementMode In the MovementMode box select the movement mode Interrupt default If the diverter valve is open Collect prior to any movement of the drop former the diverter valve will always close Drain prior to a movement and will open again Collect after the target position has been
27. ages 7 6 Replacing the Diverter Valve Diverter valve with 1 0 mm ID PEEK drop former 6702 0500 up to 150 mL min Diverter valve with 0 4 mm ID drop former up to10 mL min 6702 0300 including tools and tubing The rear cable is pre installed on the diverter valve 1 Shut down and unplug the fraction collector Set the pump flow to 0 2 Carefully move the diverter valve assembly along the Y arm to the front 3 Remove the sample inlet and waste tubing and unscrew the current drop former from the diverter valve 4 Loosen the two diverter valve screws by using the hexagon wrench shipped with the kit Fig 28 Hexagon wrench Diverter valve Screws Fig 28 Dismounting the diverter valve Operating Instructions Page 67 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 Loosen the two finger tight knurled screws that attach the rear cable and grounding cable to the diverter valve assembly Fig 29 Rear cable screw Ground cable screw Fig 29 Cable screws on the diverter valve assembly 6 Disconnect the diverter valve cable from the auxiliary I O connection on the AFC 3000 rear panel Fig 2 7 Loosen the finger tight knurled screw that attaches the rear cable to the mounting block on the AFC 3000 rear panel Fig 30 Fig 30 Removing the rear cable screw 8 Reinstall the new diverter valve in reverse order If you are replacing a low flow diverter valve als
28. al Converter and USB Serial Port are usually found and installed automatically see below If this is not the case install the drivers manually as described above under nstalling the Virtual COM Port on the Chromeleon Computer Windows Vista Windows 7 and Windows Server 2008 will automatically detect the fraction collector and perform the USB installation The message Your device is ready to use appears When you open the message you can see the number of the new virtual COM port e g COM3 Windows XP will automatically detect the fraction collector and launch the Found New Hardware Wizard which guides you through the installation of the USB Serial Converter If asked whether Windows can connect to Windows Update to search for software select Yes this time only If no internet connection is available you must manually install the drivers as described in section 2 2 Click Finish when the wizard reports that the software has been installed The Found New Hardware Wizard is launched again Repeat the same steps for installation of the USB Serial Port Operating Instructions Page 37 UltiMate 3000 Series AFC 3000 Automated Fraction Collector ne WEN m EA e e AUXILIARY on E POWER bes USB COM1 Power External Power Supply Fig 13 Rear panel connections on an UltiMate 3000 system with AFC 3000 E
29. ality and fraction collection quality e If more than one detector is used enter the delay time offset between the first detector after the separation column and the subsequent detector or detectors under Detector Delay Offsets Program Wizard Fraction Collection Delay and Detector Offset Options Delay Delay time 5 0 0 0 9999 0 s C Delay volume o The delay measured in s or ul is defined between the first detector and the collection device Detector Delay Offsets Detector Name Delay Time Offset s Fig 23 Fraction Collection Wizard Delay and Detector Offset Options page After completion of the wizard Chromeleon creates a program file method Use this program as a basis and add the required AFC 3000 commands and properties for example by copying them from the example program provided in the appendix Operating Instructions Page 55 UltiMate 3000 Series AFC 3000 Automated Fraction Collector To modify a program 1 Open an existing program Select and double click the program you want to open On the File menu select Open In the dialog box select Program on the Object of Type list and select the program Chromeleon Program File Example Commands L File Edit View Workspace Qualification Control Window Help Oa BHS k Title Example program for fraction collection with the amp FC 3000 Automated Fraction Collector Commands Jy V Post acquisition st
30. and Preventive Maintenance Routine maintenance of the AFC 3000 consists of regular cleaning of specific fraction collector components Routine maintenance also includes checking AFC 3000 components for leaks or other damage Additional periodic maintenance tasks may be required including replacement of the following fraction collector components sample inlet tubing rinse station tubing and fraction tray Perform the maintenance procedures listed in the following table at regular intervals to ensure optimum performance and maximum uptime of the fraction collector The exact maintenance schedule for the fraction collector will depend on numerous factors and will vary by application Frequency What you should do Check the fluid lines for any signs of leakage page 63 Check the fluid connections for any signs of salt deposits Clean the fraction collector general page 62 When using buffer solutions flush the fraction collector flow path thoroughly after use with a suitable solvent Weekly Clean the fraction collector thorough page 63 Periodically Inspect the tubing for possible damage such as cracks nicks cuts or blockages Replace rinse station tubing and sample inlet waste tubing connected to the diverter valve as needed Check the rinse station tubing connected to the rinse station Verify that the tubings are not clogged and are routed to the appropriate drain system or waste container without trap
31. c koc ko K K KK ke kk kc KK ck kk ck ck AK K ke ke kc e ke ke e ke ke kc ke kk ck ck kk kk ke ke e e KK Sample run ends here prkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkk kkkkkkkkkkkkkkkkkkk kkkkkkkkkkkk Page 80 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 11 Index Diverter Valve Assembly sss 27 A Adjust Height 33 A Alignment dni ea S Ros 44 eis vai Mount Assembly 27 Optional i i eene 74 i Diverter Valve Connector seee 19 Standard ite RA 73 h Demut Rises ie eh nearer REESE 29 Alignment x caedi RE 44 Auxiliary I O C i 19 Drip Tray Tubung eee 29 HESS S e NN nomo dau Drop e 15 33 44 e E Chromeleon 2e manie dis 21 45 AB d Comm c orne ter 52 Example Program 77 Bath RB e 57 F CollectMode essere 50 Connect inserer IB end 46 Facility Requirements sse 23 Direct Control 46 Firmware Update 69 Example Program 77 Flush Rinse Station 57 Fraction Collector Configuration 40 Found New Hardware Wizard 37 Fraction Collector Installation 39 Foxy Racks eoi ense ERU 32 Installation Program 39 Fraction Collection Driver 39 52 TACENSE amp cero Rte te OR ete t 21 Fraction Collection Wizard ee 52 MovementMode seen 51 Fraction Tube Racks 20 30 49 Program Start 57 Fra
32. cause damage to the system e Use only the capillaries shipped with the fraction collector and original Dionex spare capillaries Connect the waste and sample inlet tubing to the diverter valve Verify that the tubings are properly connected and tightened Waste Sample inlet 6 ef lt 5 vo Fig 5 Connections on the diverter valve Note the following when connecting the sample inlet In order to make sure that the capillary end is not pushed into the valve bore place the ferrule at the capillary end so that the capillary does not protrude from the ferrule Fig 6 Then carefully install the capillary imm D M right Wrong Fig 6 Connecting the sample inlet tubing to the diverter valve Page 28 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 3 3 Attaching the Rinse Station The rinse station 1s located at the extreme left position of the fraction collector head Fig 1 page 17 This station is used to dispense liquid to waste through normal operation of the HPLC system operation The dispensed liquid is evacuated by gravity drain via a gravity drain arrangement into a waste reservoir The rinse station is installed in its holding bracket at shipment but can be removed easily by pulling the station out of the bracket The rinse station easily snaps back into place i Tip Ensure that the height of the drop former and rinse station is properly adjusted
33. ction Tubes 20 Rack Configuration 49 Front Panel ooo eo eo 17 Server Configuration Program 39 Server Monitor 39 I Son Dialog Boxes Inspect Components seee 43 Eege ee eret RENE 41 Installation 26 Dionex AFC 3000 Fraction Collector 40 st Os ment en ON n PA E i in Chromeleon sse 39 Gleaming sme ste 62 Power Connection 35 Daily te met etes 62 Teste et dto od Rs eee red 43 Weekly Hart en tte ee ne 63 f Verification 43 Collect Mode 5 sies 50 Intended Use 2 10 Commands Dialog Box 46 Interface Test 43 E E ed e ed s eA ne 40 ELE Configurations 15 K Connection re ee Re n 35 Chromeleon Computer 36 Kit for Low Flow Rates 15 66 Diverter Valve sees 28 Kit for Normal Phase LC 15 POWOE Renan WO Piment 35 RS 232 Cable einen ette 36 L USB Cable esce cepto e mieten an 36 Control Automated 45 52 Mau a eo E e Control Direct caserne 2 EASQUE EE Control Panel ineo 47 M D Maintenance 45 58 Daily Cleaning sere een aes 62 EE 2 j M SSAB S ai cce tree EEN Qe d 60 Diverter Valve eee 28 67 Movement Mode 51 Connection Sen zsz teet nete eet dvor de 28 Replace unde RECEN 67 Operating Instructions Page 81 UltiMate 3000 Series AFC 3000 Automated Fraction Collector N Normal Phase LC 15 64 O Operating Instructions eee 1 Operation senes eres 45 Chtromeleem seed eer 45 Safety Pr
34. d instrumentation analytique vous devez connaitre les risques d utilisation de produit chimiques i Veuillez noter Avant de commencer utiliser l instrument assurez vous que vous vous tes familiaris s avec le contenu de ce manuel D Avertissement Toutes les personnes utilisant l instrument doivent observer les consignes de s curit suivantes et dans les autres chapitres de ce manuel pour viter une mise en danger de sa personne ou de dommage l instrument pendant l utilisation et des op rations de maintenance ou service de l instrument Observez les tiquettes d avertissement sur l instrument et r f rez vous aux sections correspondantes dans ce mode d emploi e Equipment de protection Pour tous les travaux sur le syst me HPLC ou proximit portez l quipement de protection personnel v tements de protection gant de s curit lunettes de protection qui correspond aux risque d coulant de la phase mobile et ou de l chantillon Pour les informations sur la manipulation correcte des compos s et des recommandations pour les situations de risque sp cifiques veuillez consulter la fiche de donn es de s curit des substances que vous utilisez Veuillez respecter des directives des Bonnes Pratiques de Laboratoire BPL Une installation permettant de se laver les yeux ainsi qu un lavabo doivent se trouver proximit du syst me Si une substance quelle qu elle soit entre en contact avec vos yeux ou votre peau
35. device must observe the following safety precautions and all additional safety precautions in this manual to avoid the possibility of personal injury or damage to the device when operating the device or carrying out any maintenance or service procedures Observe any warning labels on the device and refer to the related sections in these Operating Instructions e Protective equipment When performing any work on or near the HPLC system wear personal protective equipment protective clothing safety gloves safety glasses as required by the hazard of the mobile phase and sample For information about the proper handling of a particular substance and for advice on specific hazards refer to the material safety data sheet for the substance you are using Observe the guidelines of Good Laboratory Practice GLP An eyewash facility and a sink should be close to the device If any substance splashes on the eyes or skin wash the affected area and seek medical attention Operating Instructions Page 3 UltiMate 3000 Series AFC 3000 Automated Fraction Collector e Hazardous substances Many organic solvents mobile phases and samples are harmful to health Be sure that you know the toxic and infectious properties of all substances that you are using You may not know the toxic or infectious properties of many substances that you are using If you have any doubt about a substance treat it as if it contains a potentially harmful substance Fo
36. e fraction collector will not function properly if the level of radioactivity is above background 3 2 Unpacking All electrical and mechanical components of the fraction collector are carefully tested before the instrument is shipped from the factory After unpacking please inspect the instrument for any signs of mechanical damage that might have occurred during transit i Tips Immediately report any shipping damage to both the incoming carrier and Thermo Fisher Scientific Shipping insurance will compensate for the damage only if reported immediately Keep the original shipping container and packing material They provide excellent protection for the instrument in case of future transit The product warranty will not be honored if the fraction collector is shipped in any other packaging AN Important Ship the unit only in the original shipping container and observe the packing instructions Shipping the unit in any other packaging automatically voids the warranty For more information see the warranty statement in the terms of sale A Important Exp dier l unit uniquement dans le conteneur d exp dition original et d observer les instructions d emballage L unit dans toute autre emballage automatiquement d exp dition la garantie en vides Pour plus d informations consultez la d claration de garantie dans les conditions de vente Page 24 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collecto
37. e tubes might be disturbed It is therefore important to test it before you actually run samples with the fraction collector i Tip Before testing the alignment of the drop former ensure that you have installed all fraction collector components correctly Also ensure that you have securely tightened all finger tight screws Testing the drop former involves observing its operation To do so complete the following steps 1 Load the universal drip tray with the rack type and tubes intended to be used section 3 3 5 2 Turn the fraction collector power switch on and verify that the LED power indicator is on blue 3 Set the correct rack configuration in Chromeleon section 5 2 3 page 49 4 In Chromeleon open the Commands dialog box page 46 Click the plus sign beside AFC and then click TubePosition 5 Inthe TubePosition box select a suitable position for example position 1 and click Execute to move the drop former to the designated dispense position Check that the drop former tip correctly accesses each position at the center of the tube Repeat for further suitable positions as needed i Tip Ifthe fraction collector alignment is not correct contact Thermo Fisher Scientific Service for Dionex HPLC Products Page 44 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 Operation and Maintenance The AFC 3000 is both reliable and easy to use Before using it ho
38. ecautions 3 Safety Symbols eee 2 Operation Principle esee 14 P Panel Tabie torna u S RE 47 Power Connection 35 Power SWitCz iac een 19 Powet Up assise tiennent 45 Program Creation 52 R Racks rte rh artist 20 30 49 R at Pariel shell t eet uit 18 Rear Panel Connector ssesessseseeesseseesseeee 19 36 Repair Jta eie ehe 61 General Notes 4x34 ettet e eR 61 Replace Diverter Valve 67 Rinse Station sese tet eter tene 29 57 Rinse Station Tubing sss 29 RMA Number 62 RS2 232 Port ceret aote Eegen 19 S Safety Information 2 Safety Precautions sss 3 Safety Symbols 2 Serial Communication cccceeceeseeceseeeeeeeees 41 Serial Portas etes eed ee eU 19 Server Configuration Program 39 Server Monitor 39 None c EE 61 Check for Leaks 63 Clean Fraction Collector 62 Firmware Update 69 General Notes 61 Install Low Flow Kit 66 Install Normal Phase Kit 64 Replace Diverter Valve ee 67 Safety Measurements eee 61 Shutdown eee een nn 57 Software Control 21 45 Solveuts sind nn mae dens 10 Spare Parts ouo rrr odes 75 Specifications sereine e de 71 Stainless Steel Drop Former 15 64 Startup o reset Re EE PER ERU CH 45 T Technical Information
39. ent dans l espace de travail Si la phase mobile ou l chantillon contient des solvants volatils ou inflammables vitez les flammes nues et les sources d tincelles proximit e D charge lectrostatique D charge lectrostatique peut provoquer la formation d tincelles et peut pr senter un risque d incendie Veuillez noter que des solvants fluides dans les capillaires peuvent se charger automatiquement Cet effet se peut produire particuli rement forte dans les capillaires isolants et avec des solvants non conducteurs par exemple l acetonitrile pur Prenez des mesures appropri es pour viter les charges lectrostatiques proximit du syst me HPLC Par exemple s assurez qu il y a une humidit de l air suffisante et une ventilation ad quate dans le laboratoire portez des v tements ou quipement de protection antistatique vitez l accumulation de bulles d air dans les lignes de d chets et utilisez des r servoirs d chets mis la terre Utilisez uniquement des capillaires non conducteurs pour diriger solvants au r servoir de d chets Capillaires lectriquement conducteur devrait tre mis la terre Applications en phase normale N effectuez pas d applications en phase normale sans vous tre assur que votre faiseur des gouttes est proprement isol e et mise la terre Les solvants de la phase normale non conducteurs et non polaires par exemple hexane peuvent entrainer une accumulation de charges
40. ent jamais en contact avec les agents de nettoyage Ne jamais lubrifiez les deux broches filet es 3 Repeat step 2 using a towel dampened with clear water This process removes any remaining contaminants 4 Dry the fraction tray fraction collector cabinet and fraction collector arm using a dry towel The AFC 3000 must be thoroughly dry before you turn the fraction collector power on 7 2 2 Weekly Cleaning Although cleaning the fraction collector daily removes spills and contaminants from most of the fraction collector components it is necessary to clean the AFC 3000 more thoroughly once a week To do so complete the following steps 1 Shut down and unplug the fraction collector 2 Remove the rack 3 Wipe the fraction collector exterior base and tray until they are clean using a towel dampened with a lab grade cleaning agent followed by a towel dampened with clear water 4 Replace the rack on the fraction collector tray 7 3 Checking for Leaks Several of the fraction collector components have a limited life and will wear out under normal use the drop former eluent tubing and the rinse station tubing Standard maintenance procedures require that you periodically check these components for leaks To do so complete the following steps 1 Shutdown and unplug the fraction collector 2 Visually inspect the drop former eluent tubing and rinse station and tubing for leaks or signs of deterioration or salt loading
41. eps Fig 24 Chromeleon program file shown in the Commands view here not connected to a timebase Change the settings in the program as desired and add the required AFC 3000 commands A program example can be found in the appendix Click Commands to open the Commands View The Commands View shows the entire program listing the control commands in chronological order Edit the parameter of interest or enter a new parameter A Important The rack configuration Rack and the collect mode CollectMode must not be changed during the course of a program file A Important Ne changez pas la configuration du rack Rack et le mode de collection CollectMode au cours d un programme 3 To start the program follow the steps below For more information on fraction collection see the Chromeleon Help Page 56 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector To start a program Program for sample analysis 1 Create a sample list sequence A sequence must include the program and a method for evaluating the sample data for example for peak identification area determination and amount determination 2 Assign the program and method to each sample on the list 3 Add the sequence to the batch and start the batch For information about each of the above steps see the Chromeleon Help 5 3 Shutting Down the Fraction Collector To shut down the fraction collector complete the followi
42. er With electrically conductive capillaries make sure that they are properly grounded Operation with Normal Phase Eluents Do not perform normal phase applications without a proper grounding protection for the drop former Non conductive non polar normal phase eluents for example hexane can lead to a build up of electrostatic charges on an ungrounded drop former The electrostatic discharge can create a fire and or explosion hazard Note that a grounded drop former part no 6702 0400 is available for normal phase NP applications Use normal phase eluents with the AFC 3000 Automated Fraction Collector only after it has been modified with this grounded drop former Operating the device without the grounded drop former can result in a fire and or explosion hazard e Self ignition of solvents Do not use solvents for which the self ignition temperature is below 150 C In case of leakage these solvents may self ignite on a hot surface Page 4 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector Capillaries capillary connections open connections Capillaries especially non metallic capillaries may burst slip out of their fittings or may not be screwed in This may result in substances spraying out of the open connections In an UltiMate 3000 system some components are made of PEEK This polymer has superb chemical resistance to most organic solvents However it tends to swell whe
43. er cords provided for the device Do not use multiple sockets or extension cords Using defective multiple sockets or extension cords may cause personal injury or damage to the device D Avertissement Utilisez uniquement les cordons d alimentation lectrique sp cifique Pinstrument N utilisez pas des blocs multiprise ou des c bles prolongateurs Cela pourrait entra ner des blessures corporelles ou endommager l instrument A Important The AFC 3000 is intended to operate from an AC power source that will not apply more than 240V AC between the supply conductors and ground A protective ground connection by way of the grounding connector in the power cord is required for safe operation A Important L AFC 3000 est destin a fonctionner d une source d alimentation courant alternatif qui s appliquera pas plus que 240V courant alternatif entre le conducteur et la terre Une connexion de protection a la terre par le fil neutre dans le fil d alimentation est n cessaire pour la s curit d exploitation Operating Instructions Page 35 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 4 2 General Communications Information To connect the fraction collector to the Chromeleon computer select one of the following alternatives e Use an RS 232 cable part no 8914 0153 shipped with the instrument to connect the COM 1 port on the rear panel of the fraction collector to a serial COM port on the computer secti
44. er with grounding cable D Warning Do not perform normal phase applications without a proper grounding protection of the drop former Non conductive non polar normal phase eluents for example hexane can lead to a build up of electrostatic charges on an ungrounded drop former The electrostatic discharge can create a fire and or explosion hazard Operating Instructions Page 15 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Q Avertissement N effectuez pas d applications en phase normale sans vous tre assur que votre faiseur des gouttes est proprement isol e et mise la terre Les solvants de la phase normale non conducteurs et non polaires par exemple hexane peuvent entrainer une accumulation de charges lectrostatiques sur le faiseur des gouttes en l absence de mise la terre Le d charge lectrostatique peut repr senter un risque d incendie et ou d explosion For information about the optional accessories that are available for the fraction collector see section 9 2 page 74 Page 16 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 4 Front Panel Elements Fig 1 Front panel view No Front Panel Element Description 1 Power indicator LED The LED is illuminated blue when the fraction collector is turned on 2 Fraction collector arm Transports the diverter valve assembly fore and aft see also section 2 2 page 14 3 Diverter
45. es the following axis components Axis Component Description When facing the front of the unit the left to right movement axis of the fraction collector arm When facing the front of the unit the fore and aft movement axis of the diverter valve assembly on the fraction collector arm The manually adjustable height axis of the diverter valve assembly Page 14 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 3 Fraction Collector Configurations The fraction collector is available in the following standard configuration for flow rates up to 150 mL min AFC 3000 Automated Fraction Collector including 5702 1000 two 90 position racks 180x 8 mL tubes 13 mm OD 1 6 mm ID diverter valve and 1 mm ID PEEK drop former Kit for low flow rates A kit for flow rates up to 10 mL min is available It includes a different diverter valve which replaces the standard valve Kit for low flow rates including 6702 0300 1 0 mm ID diverter valve with 0 4 mm ID PEEK drop former Kit for normal phase LC In addition to the standard and low flow PEEK drop formers Thermo Fisher Scientific offers a stainless steel drop former with 1 0 mm ID for use with normal phase solvents The stainless steel drop former provides grounding via the diverter valve cable and is suitable for flow rates up to 150 mL min Kit for normal phase LC including 6702 0400 1 0 mm ID stainless steel drop form
46. es to be used for collecting a single fraction in the Max number of tubes per fraction box e Type the total number of installed tubes in the Total number of tubes box e Program Wizard Fraction Collection Collection Device Options X Collecting Pump Select the pump device that delivers the flow to the collecting device Pump device Pump DI Collecting Device Parameters Max tube volume Unlimited Unlimited 1000 000000 ml Max number of tubes per fraction Unlimited Unlimited 999 Total number of tubes Unlimited Unlimited 9999 Minimum time for tube change 2 0 0 0 100 0 s After last tube retum to first r Fig 21 Fraction Collection Wizard Collection Device Options page Operating Instructions Page 53 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Fraction Collection Peak Detection Options Modify the peak detection algorithm for fraction collection if required Fig 22 For more information see the Chromeleon Help Info These are the parameters for the peak detection algorithm used collection s Program Wizard Fraction Collection Peak Detection Options Peak Start Conditions Jl Peak Start True Time noos Peak End Conditions Jl Peak End True Time noos Time Step for Slope and Curve Dervatvestep mos SpeciaPeakEndThreshod Threshold DoNotResove Off Peak Max C
47. ette DEEE D chets d Equipements Electriques et Electroniques Pour plus d informations r f rez vous au chapitre WEEE Information dans le classeur Installation and Qualification Documents for Chromatography Instruments Page 2 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector At various points throughout the manual messages of particular importance are indicated by certain symbols i Tip A Important A Important Q Warning D Avertissement Indicates general information as well as information intended to optimize the performance of the instrument Indicates that failure to take note of the accompanying information could cause wrong results or may result in damage to the instrument Indique que ne pas tenir compte de l information jointe peut conduire de faux r sultat ou endommager l instrument Indicates that failure to take note of the accompanying information may result in personal injury Indique que ne pas tenir compte de l information jointe peut entra ner des blessures corporelles 1 2 2 Safety Precautions When working with analytical instrumentation you must know the potential hazards of using chemical solvents i Tip D Warning Before initial operation of the fraction collector make yourself familiar with the contents of this manual For the safety precautions in French see page Fehler Textmarke nicht definiert All users of the
48. fting or moving the fraction collector When lifting or moving the autosampler always lift by the bottom or sides of the unit This is to avoid damage to the instrument After operation rinse out buffers and solutions that form peroxides Before switching from buffer to organic solution rinse the analytical system thoroughly with deionized or HPLC grade water When switching to another solvent ensure that the new solvent is miscible with the one contained in the HPLC system If the solvents are not miscible the system can be damaged for example by flocculation Operating Instructions Page 5 UltiMate 3000 Series AFC 3000 Automated Fraction Collector e Ifaleak occurs turn off the fraction collector immediately stop the pump flow and remedy the situation e Use only standard solvents HPLC grade and buffers that are compatible with all parts that may be exposed to solvents e To avoid personal injury do not reach inside the sample area during a running analysis e Before interrupting operation for several days or more or when preparing the fraction collector for transport observe the precautions for shutting down the instrument page Fehler Textmarke nicht definiert e Do not use the fraction collector in ways other than those described in these Operating Instructions e Keep the operating instructions near the device to be available for quick reference 1 2 3 Consignes de S curit Si vous utilisez
49. gned or create a new timebase on the Edit menu click Add Timebase Open the Add device to timebase dialog box To do so click Add Device on the Edit menu or right click the timebase and click Add Device on the menu On the Manufacturers list click Dionex and on the Devices list click AFC 3000 Fraction Collector There are three configuration tab pages Make the required settings on the first two tab pages For more details see section 3 5 2 10 Click OK to complete the installation of the fraction collector Operating Instructions Page 39 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 11 Open the Add device to timebase dialog box again On the Manufacturers list click Generic and on the Devices list click Fraction Collection Accept the settings on the two configuration pages then click OK 12 On the File menu click Save Installation and then close the Server Configuration program 3 5 2 Configuring the Fraction Collector This section describes the settings that must be made on the first two configuration pages Dionex AFC 3000 Fraction Collector Page On this page you can activate or deactivate the Demo Mode Dionex AFC 3000 Fraction Collector Dionex AFC 3000 Fraction Collector AFC Error Levels Demo Mode Fig 14 Dionex AFC 3000 Fraction Collector page e Demo Mode Verify that the check box is cleared In the Demo Mode Chromeleon simulates the functions of the fraction collector
50. ice for Dionex HPLC Products 6 2 Power and Communication Problems The following table provides information about operating problems and lists probable causes as well as remedial actions Probable Cause Remedial Action The blue LED The instrument is not connected Connect the power cord power indicator is to the mains section 3 4 1 page 35 ff S The power is turned off Turn on the fraction collector power The power switch of the external Turn on the power switch of the power supply is turned off external power supply if equipped An error occurred in the Contact Service electronic system The AFC 3000 does There is no connection between Check the USB or RS 232 cable and not work correctly the fraction collector and the connection to the computer If the cable when controlled by Chromeleon computer is connected ensure that it is tightened Chromeleon properly Verify that the cable is connected to COM 1 port on the AFC 3000 rear panel or to the appropriate module USB hub if connecting in that manner section 3 4 page 35 Test the interface section 4 3 page 43 Operating Instructions Page 59 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Problem Probable Cause Remedial Action The AFC 3000 does The fraction collector is not Check the configuration for the correct not work correctly correctly configured in COM port selection when controlled by Chromeleon section 3 5 2
51. indows Vista are registered trademarks of Microsoft Corp All other trademarks are property of Thermo Fisher Scientific Inc and its subsidiaries Operating Instructions Page 1 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 1 2 Safety Information The CE Mark label on the instrument indicates that the instrument is compliant with the related standards page I 1 2 1 Symbols on the Fraction Collector and in the Manual The table below shows the symbols used on the fraction collector Description Direct current Courant continu Power supply is on L instrument est mis sous tension and Power supply is off O L instrument est mis hors tension O Pinch point hazard Risque de pincement Refer to the Operating Instructions to prevent risk of harm to the operator and to protect the instrument against damage R f rez vous ce manuel pour viter tout risque de blessure l op rateur et ou prot ger l instrument contre tout dommage Label according to the Measures for Administration of the Pollution Control of Electronic Information Products China RoHS guideline tiquette Measures for Administration of the Pollution Control of Electronic Information Products China RoHS WEEE Waste Electrical and Electronic Equipment label For more information see the WEEE Information section in the Installation and Qualification Documents for Chromatography Instruments binder tiqu
52. ks installed select NotUsed for all unused rack positions Note the following restrictions e All racks have to be installed and defined following the order from Rack1 to Rack6 e Unused rack positions NotUsed must always be the last positions For example it is not allowed to set NotUsed for Rack1 and then select a rack type for Rack2 Observe the rack layout scheme example for four 96 well plates see Fig 18 details for all rack types see page 31 e Racks for 96 well plates must not be mixed with any other rack types 4 Click Execute Fig 18 Rack layout scheme here four 96 well plates Tube numbering assumes SawVertical collect mode Operating Instructions Page 49 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 2 4 Defining the Collect Mode Define how the drop former travels across the racks A Important The CollectMode must not be changed during fraction collection or during the course of a program file or sequence A Important Ne changez pas le CollectMode au cours de la collection de fraction ou au cours d un programme ou s quence 1 Open the Commands dialog box page 46 2 Click the plus sign beside AFC 3 Click CollectMode In the CollectMode box select the collect mode Vertical The drop former follows a meander shaped path Be aware that this mode minimizes tube change times but negates imprinted tube numberings SawVertical default The drop former follows
53. l service and repair procedures observe all precautionary statements provided in these operating instructions e When performing maintenance or repair work on the fluidic components always move the diverter valve assembly into the appropriate position to facilitate safe working conditions and to allow access to all components without obstructions The power should be turned off for almost all service procedures to ensure the safety of the operator technician Follow the instructions in the sections for the individual components e Use only the original spare parts authorized for the device by Thermo Fisher Scientific e Before returning any instrument to for repair contact Thermo Fisher Scientific Service for Dionex HPLC Products An RMA Return Material Authorization number is required to track your instrument Always use the original shipping container and observe the packing instructions when shipping the fraction collector Shipping the instrument in anything other than the original packaging voids the warranty If the appropriate shipping container is not available appropriate shipping containers and packing material can be ordered from the Thermo Fisher Scientific sales organization for for Dionex HPLC Products The packing instructions are included in the Installation and Qualification Documents for Chromatography Instruments binder and are available on request For more information see the warranty statement in the terms of sale
54. lectrostatiques sur le faiseur des gouttes en l absence de mise la terre Le d charge lectrostatique peut repr senter un risque d incendie et ou d explosion Operating Instructions Page 7 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Notez que un faiseur des gouttes isol e et mise la terre est disponible pour applications en phase normale r f rence 6702 0400 Utilisez les solvants de type phase normale uniquement avec le collecteur de fraction AFC 3000 modifi l aide du cette faiseur des gouttes isol e et mise la terre L utilisation du collecteur de fraction sans le faiseur des gouttes isol e et mise la terre peut presenter un risque d incendie et ou d explosion e Inflammation spontan e des solvants N utilisez aucun solvants avec une temp rature d auto inflammabilit inf rieure 150 C Si une fuite se produit ces solvants peuvent s auto enflammer au contact d une surface chaude e Capillaires connecteur capillaires connexions ouvertes Des capillaires en particulier les capillaires non m talliques pourraient fendre ou glisser des connecteurs ou ne peuvent pas tre viss s Ceci peut en r sulter aussi que des substances pourraient jaillir des connexions ouvertes Dans un syst me UltiMate 3000 certaines composantes sont en PEEK Bien que ce polym re pr sente une excellente r sistance chimique la plupart des solvants organiques il a tendance gonfler lorsqu il est
55. lve e 28 3 3 3 Attaching the Rinse Station sis e ote iet areata eere apte tede aa dot e 29 3 3 4 Connecting the Drain Tubing ioi o pe ER eg tpi te ape Red 29 3 3 5 Placing Fraction Racks on the Universal Drip Tray sesssss 30 3 3 6 Adjusting the Drop Former Heights 33 3 4 Connecting the Fraction E 35 3 4 1 Connecting the External Desktop Power Supply 35 3 4 2 General Communications Information 0 cccccecccceseceseeeeeeeeeeeeeneceteeeeeeensees 36 3 4 3 Communication via RS 23 2685 os er e RE nr en Ravens 36 3444 Communication via USB ee nids 36 3 5 Setting Up the Fraction Collector in Chromeleon esses 39 3 5 1 Installing the Fraction Collector iso dates e ER ee edet ed 39 3 5 2 Configuring the Fraction Collector qe e eee ne 40 4 Verifying Stallone sis nn tnt nes ntm sn ee sind 43 Mil OVERVIEW eege od na skladu sak dt 43 4 2 Inspecting Components Prior to Fluidic Operation 43 Operating Instructions Pagei UltiMate 3000 Series AFC 3000 Automated Fraction Collector 43 Testme the Interfaces c ue dore ei onte tei be fecu io ductae lode aule 43 4 4 Testing the Drop Former Alignment cedi e tote prae isse EEN 44 5 Operation and Maintenance eco eo sero be oret os eo obese uve vetu o revu lo jeecae obe Cope bU Pa eege 45 Sel GE EI EE 45 5 2 Operation with Chromeleon m tdi e rix reed i bo cac eR Cede een a 45 S2 Confec ngs to EE EE 46 5 2 2 lt Direct Control inneni E CE 46 5 2 3 Setting
56. mation about a page click Help Fraction Collection General Options Determine whether fractions are collected by peak or by time e f you select Collect by peak using channel choose the detection channel from the list Select the Collect outside peaks too check box to collect sample in between peaks e f you select Collect by time type a time period in the Collection period box e Under Collect Time Frame you can restrict the time for collecting fractions Click Collect only from and type or select the start and end times in the boxes Program Wizard Fraction Collection General Options D Fraction Collection Mode Off do not collect at all Collect by peak using channel v C Collect by time Collection Time Frame C No Start Time min End Time min ERR 0 000 30 000 Info Collect fraction if a peak is detected or collect by time using a fixed collection period Collect during data acquisition or restrict the collection range Fig 20 Fraction Collection Wizard General Options page Page 52 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector Fraction Collection Collection Device Options Enter collection parameters such as the maximum tube volume maximum number of tubes per fraction or total number of tubes e Type the volume of the installed tubes in the Max tube volume box e Type the maximum of number of tub
57. ment les cordons d alimentation lectrique sp cifique l instrument e Utilisez seulement des pi ces de rechange originales et des accessoires autoris s par Thermo Fisher Scientific Page 8 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector e R glez toujours une limite de pression minimum pour la pompe HPLC Ceci pr vient les dommages r sultant de fuites ou de long terme fonctionnement sec de la pompe e Le collecteur de fraction p se plus de 8 kg 17 7 livres Par cons quent soulevez le collecteur de fraction avec pr caution e Lorsque vous soulevez ou d placez le collecteur de fraction soulevez toujours par le bas ou les c t s afin de ne pas endommager l instrument e Apr s utilisation purgez le syst me des tampons et des susceptibles de former des peroxydes e Lorsque vous passez d une solution saline un solvant organique effectuez un rin age interm diaire du syst me HPLC l eau d ionis e ou qualit HPLC e Lorsque vous passez un autre solvant assurez vous que le nouveau solvant soit miscible avec celui qui se trouve dans la pompe Dans le cas contraire la pompe peut tre endommag e par exemple par des floculations e Siune fuite se produit arr tez imm diatement l instrument stoppez le d bit de la pompe et rem diez au probl me e Utilisez uniquement des solvants qualit HPLC et des solutions salines compatibles avec les mat riaux ex
58. n be operated with the UltiMate 3000 or any other U HPLC system The design has been optimized for robust and easy use as well as for minimized carry over volume A wide range of fraction collection formats and a flow range up to 150 mL min guarantees a maximum of flexibility A universal drip tray accommodates a large variety of fraction collection racks and collects spilled solvent For detailed information about the supported rack types see section 2 6 page 20 The universal drip tray includes an integrated drain at its front left corner to guide spilled solvents into a waste container The diverter valve is mounted to a height adjustable plate to allow closest possible positioning over a fraction collection tube and to cut down the carry over volume to a minimum A field upgradable grounded drop former is available for normal phase NP applications The fraction collector is designed for easy access to the fluidic components allowing fast and reliable maintenance All parts that may be exposed to solvents are made of materials that provide optimum resistance to the most commonly used solvents and buffer solutions The fraction collector is controlled by the Chromeleon Chromatography Management System Connection of the fraction collector to the Chromeleon computer is achieved by using either a RS 232 serial or USB cable Operating Instructions Page 13 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2
59. n in contact with trichlormethane CHCl5 dimethyl sulfoxide DMSO or tetrahydrofuran THF In addition it is attacked by concentrated acids such as sulfuric acid and nitric acid or a mixture of hexane ethyl acetate and methanol In both cases capillaries may start leaking or they can burst Swelling or attack by concentrated acids is not a problem with brief flushing procedures Do not use tubing that is stressed bent kinked or damaged Capillary connections can be contaminated by harmful substances or harmful substances can escape from open connections Always wear safety glasses when handling fused silica tubing for example during installation or when cutting capillaries to the length Disconnect the fraction collector from all power sources before removing the panels When the panels are removed dangerous electrical connections will be exposed The enclosure must be opened only by Thermo Fisher Scientific service personnel Replace faulty communication cables Replace faulty power cords Never use a power cord other than the power cords provided for the device Use only the original spare parts and accessories authorized for the device by Thermo Fisher Scientific When operating the HPLC system always set a lower pressure limit for the pump This prevents damage resulting from leakage or from running the pump dry The fraction collector weighs more than 8 kg 17 7 lbs Therefore you should use caution when li
60. nce the descriptions The descriptions in this manual refer to firmware version 1 03 and Chromeleon 6 80 Service Release 13 This manual is provided as is Every effort has been made to supply complete and accurate information and all technical specifications have been developed with utmost care The information contained in this manual should not be construed as a commitment by Thermo Fisher Scientific Thermo Fisher Scientific assumes no responsibility for any errors that may appear in this document that is believed to be complete and accurate at the time of publication and in no event shall Thermo Fisher Scientific be liable for incidental or consequential damages in connection with or arising from the use of this document We appreciate your help in eliminating any errors that may appear in this document The information contained in this document is subject to change without notice All rights reserved including those for photomechanical reproduction and storage on electronic media No part of this publication may be copied or distributed transmitted transcribed stored in a retrieval system or transmitted into any human or computer language in any form or by any means electronic mechanical magnetic manual or otherwise or disclosed to third parties without the express written permission of Thermo Fisher Scientific Inc Trademarks PEEK is a trademark of Victrex PLC Simriz is a trademark of Carl Freudenberg KG Windows and W
61. nds Lea Tube Position Instrument Tube Position Software Tube Position Home AFC Audit Trail 11 55 55 AFC Send HOME10d 11 55 56 AFC Send SOL 010d 11 55 56 AFC Send SOLMODE 010d 11 55 56 AFC Send NEXTMODE 210d 11 55 57 AFC Disconnect Ret Time 11 55 58 AFC Connect 11 55 58 AFC Send HOME10d 11 55 58 AFC Send SOL 010d e 1 55 58 AFC Send SOLMODE 00d 911 55 58 AFC Send NEXTMODE 210d Fig 17 AFC 3000 Control Panel The control panel provides access to the operating parameters and commands required for routine operation of the fraction collector Additional functions are available in the Commands dialog box To open the Commands box from the panel tabset select Command on the Control menu Page 48 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 2 3 Setting the Rack Configuration Define the installed rack types in Chromeleon It is not recommended to define a rack type in a program file A Important The rack configuration must not be changed during fraction collection or during the course of a program file or sequence A Important Ne changez pas la configuration du rack au cours de la collection de fraction ou au cours d un programme ou sequence 1 Open the Commands dialog box page 46 2 Click the plus sign beside AFC 3 Click Rack In the Rack1 to Rack6 boxes select the installed racks If you have less than six rac
62. ng steps 1 Flush flow paths as required and as outlined in section 5 4 2 Shutdown the HPLC system to which the fraction collector is connected as is required and as instructed via the associated documentation and outlined procedures therein 3 Turn offthe AFC 3000 power switch 5 4 Flushing the Rinse Station and Eluent Flow Path Generally you can operate the AFC 3000 without flushing the rinse station Under normal circumstances you can simply drain the rinse station prior to shutting down the fraction collector However you should flush the rinse station and flow path under two circumstances e during initial startup of the AFC 3000 after installation e following the use of strong bases acids or organic solvents as may be present in the eluent Flushing the rinse station during initial startup of the AFC 3000 removes any contaminants Flushing the rinse station after using strong rinse eluent agents solvents prevents degradation and failure of the flow path components If additives such as buffers are used as a part of the mobile phase flush the system with several volumes of a suitable solvent before shutting the AFC 3000 down This will prevent buildup of precipitates inside the flow path To flush the rinse station and flow path flush the entire HPLC system as outlined in the system s operating instructions Operating Instructions Page 57 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 5 Routine
63. o BR Mounting block of diverter valve assembly Page 18 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 5 1 Power Switch The power switch on the rear panel is the main power switch for the fraction collector Turn on the power switch before initial operation of the fraction collector Turn off the main power switch when instructed to do so for example before performing a service procedure or when interrupting operation for longer time periods In this case also observe the safety precautions outlined in this manual 2 5 2 USB Port You can use a USB connection to connect the fraction collector to the Chromeleon computer for example if no RS 232 COM port is available The communication data is transferred digitally via the appropriate USB cable However a USB converter software must be installed so that Chromeleon can communicate with the fraction collector To ensure trouble free operation all USB cables must be ordered from the Thermo Fisher Scientific sales organization for Dionex HPLC Products For information about how to connect the fraction collector to the Chromeleon computer see section 3 4 page 35 2 5 3 RS 232 Serial Port The Chromeleon Chromatography Management System uses a serial connection RS 232 to control the fraction collector The communication data is transferred digitally via the appropriate RS 232 cable To ensure trouble free operati
64. o see section 7 5 page 66 Page 68 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 7 7 Replacing the Universal Drip Tray For proper operation the universal drip tray must be properly seated on the base plate of the fraction collector An improperly installed universal drip tray may lead to the impression that the fraction collector is misaligned 1 Remove the rack adapter or the fraction racks from the universal drip tray as shown in Fig 11 on page 33 2 Lift the drip tray from the base plate such that the holes in the two screws in the base plate disengage from the two holes in the drip tray 3 Place the new universal drip tray on the fraction collector such that the two screws on the base plate are placed inside the holes in the tray rw Hole Screw Fig 31 Installing the universal drip tray 4 Verify that the universal drip tray is not misaligned This means that the drip tray and the base plate have to be arranged in parallel compare figures below Fig 32 Misaligned universal drip tray Fig 33 Properly aligned universal drip tray 7 8 Updating the Firmware The fraction collector is shipped with the most recent firmware version However in some cases a firmware update may be required Only qualified service personnel should perform firmware updates For more information please contact Thermo Fisher Scientific Service for Dionex HPLC Products Ope
65. on all RS 232 cables must be ordered from the Thermo Fisher Scientific sales organization for Dionex HPLC Products 2 5 4 Auxiliary UO Connection The auxiliary I O connection is used to provide power to the diverter valve and grounding to the stainless steel drop former The appropriate cable is pre installed on the diverter valve Operating Instructions Page 19 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 6 Universal Fraction Tray Racks and Tubes The fraction collector supports a large variety of fraction collection racks including most of the Isco Foxy Jr R1 and wellplate sampler racks For information about how to install a rack see page 30 The following rack types are supported Rack Adapter for Tube Size Part No 21 tubes 50 mL 30 mm x 100 mm 6702 0021 A funnel rack part no 6702 1021 in conjunction with this rack allows collecting fractions of unlimited volumes into appropriate vessels 24 tubes 30 mL 24 mm x 100 mm 6702 0024 40 tubes 20 mL 20 mm x 100 mm 6702 0040 60 tubes 14 mL 16 mm x 100 mm 6702 0060 90 tubes 8 mL 13 mm x 100 mm 6702 0090 60 tubes Foxy Jr R1 1 5 mL 5701 2023 72 tubes Foxy Jr R1 Mini tubes 18 mm OD 5701 2024 36 vials Foxy Jr R1 Scintillation vials 28 mm OD 5701 2025 2 well plates Foxy Jr R1 Two 96 well plates 5701 2021 40 vials WPS 3000 2 mL 12 mm OD 6820 4070 In addition a rack adapter part no 670
66. on 3 4 3 page 36 e Use a USB cable part no 6911 0002 shipped with the instrument to connect the USB port on the rear panel of the fraction collector to a USB port on the computer To use USB a virtual COM port has to be installed to the Chromeleon computer section 3 4 4 page 36 i Tip When interconnecting any computing devices keep the communications cables away from sources of electromagnetic or radio frequency RF interference such as electric motors transformers fluorescent light ballasts or RF energy sources 3 4 3 Communication via RS 232 To establish connection via a serial RS 232 cable 1 Connect the cable to COM 1 serial RS 232 port on the rear panel of the fraction collector Fig 2 2 Connect the cable to a serial RS 232 port on the Chromeleon computer 3 4 4 Communication via USB Before you can operate the fraction collector with Chromeleon via USB an additional virtual COM port must be created on the Chromeleon computer Select this new COM port when configuring the fraction collector in Chromeleon page 41 The required drivers are usually found and installed automatically when the fraction collector is plugged in If this is not the case complete the following steps Installing the Virtual COM Port on the Chromeleon Computer 1 Locate the Drivers USB Virtual COM Port folder on the Chromeleon Service Release DVD 2 Check which files are contained in the folder the shipped drive
67. onditions Peak Max Sope J of Peak Max True Time 1008 n Fig 22 Fraction Collection Wizard Peak Detection Options page Page 54 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector Fraction Collection Delay and Detector Offset Options Set the delay time or delay volume between the first detector in the system and the fraction collector e Delay Time This is a constant value which should only be applied with a constant pump flow rate of your method Note that changing the flow rate requires an according adaption of the delay time to allow for proper fraction collection Delay Volume This is the fluidic volume travelled by a peak after triggering the set start threshold conditions between a detector and the separation point of the diverter valve Using the delay volume offers more flexibility as Chromeleon automatically calculates the delay time from the pump flow rate and the defined delay volume Both the delay volume and the delay time should be determined experimentally with a suitable method As a first approximation the delay volume can be determined as a result of the volume calculation of all tubing between the detector cell and the diverter valve Note that the volume of the drop former itself does not contribute to the delay volume but to the carry over volume It is recommended to perform a suitable test with some collected fractions to assure proper function
68. ot already running page 39 2 Verify that the cable is properly connected 3 Start the Chromeleon client by clicking the Chromeleon icon Se If the Chromeleon icon is not on the desktop click Start on the taskbar point to Programs or All Programs depending on the operating system point to Chromeleon and then click Chromeleon 4 Open the Chromeleon Browser and double click the UltiMate 3000 AFC 3000 pan control panel in the Dionex Templates Panels Dionex LC folder Operating Instructions Page 43 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 Connect the control panel to the timebase in which the fraction collector is installed On the Control menu select Connect to Timebase and then select the timebase on the Timebase tab For information about the Timebase dialog click Help 6 Verify that the fraction collector is connected to Chromeleon If it is not click Connect The fraction collector resets its position with the diverter valve assembly moving in defined in X and Y direction and back over the rinse position If the fraction collector does not reset see the Troubleshooting section page 59 4 4 Testing the Drop Former Alignment The drop former must align to the center of each fraction tube to ensure satisfactory sample dispensing Shipping or rough handling can disturb the fraction collector s cabinet to base alignment If it is incorrectly aligned the fraction collection into th
69. p Former Height 1 Grasp the diverter valve assembly firmly by the vertical stainless steel element while rotating the adjustment locking lever upward This will unlock the slider assembly allowing you to move it up and down freely Fig 12 2 Slide the assembly so that the drop former is several millimeters above the highest point of the fraction tubes or vials Adjust as needed 3 Grasp the diverter valve assembly firmly by the vertical stainless steel element while rotating the adjustment locking lever downward This will lock the diverter valve assembly so that its height is fixed If the lever cannot be moved downward easily the diverter valve assembly may not be positioned properly in one of the grooves Operating Instructions Page 33 UltiMate 3000 Series AFC 3000 Automated Fraction Collector i Adjustment locking lever a NM Diverter valve e 4 4 Drop former Fig 12 Diverter valve assembly with drop former d 4 Slide the rinse station up or down in its holder so that the lowest part of the drop former is slightly above the top of the rinse station when it is in the rinse position i Tip This setting ensures that the diverter valve assembly will not impinge on the rinse station during movement while allowing for the minimum distance between those two components to minimize the risk of eluent being dispensed outside of the rinse station barrel In case of an obstruction during the movement of
70. ping liquid in the tubing which could cause an overflow condition Check the volume of the liquid in the waste container and empty as needed Check the following components for wear and replace if necessary e Drop former e Diverter valve e General tubing If you fail to replace these components when they deteriorate the fraction collector will not function properly Annually Have Thermo Fisher Scientific Service check the fraction collector once a year to prevent for example contamination and excessive wear Page 58 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 6 Troubleshooting 6 1 General Notes The AFC 3000 is both easy to operate and reliable When the fraction collector does not function properly isolate the problem to determine if it originates in the Chromeleon computer the analytical instrument the communications cable or the fraction collector itself The following sections help you to identify and eliminate the source of problems that may occur during the operation of the AFC 3000 If you determine the problem is in the AFC 3000 check the power system the communications interface or the diverter valve assembly to locate the cause of the problem and resolve it If a problem occurs always check the Chromeleon audit trail for messages section 6 3 If you cannot solve a problem using the steps given in this chapter please contact Thermo Fisher Scientific Serv
71. played The port selection list is unavailable when Chromeleon is in demo mode Operating Instructions Page 41 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 42 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 4 Verifying Installation 4 1 Overview of Actions Once the installation of the AFC 3000 has been completed it is important to verify the correct installation to ensure proper functionality of the fraction collector Installation verification of the fraction collector consists of three parts e Visual inspection of all components e Ensuring that the communications interface between the fraction collector and the Chromeleon computer is working e Ensuring that the diverter valve assembly functions properly This chapter explains how to test the above items before you use the fraction collector 4 2 Inspecting Components Prior to Fluidic Operation Complete the following steps 1 Shut down and unplug the fraction collector 2 Visually inspect the drop former rinse station and all attached tubing for leaks or signs of damage If you detect a leak or other damage to a fraction collector component you must replace the component For more information see the Service section page 61 4 3 Testing the Interface Test the communications interface between the fraction collector and the Chromeleon computer 1 Start the Chromeleon Server if it is n
72. pos s phase mobiles e Afin d viter des blessures corporelles ne mettez pas la main l int rieur du compartiment chantillons lorsqu une analyse est en cours e Avant d interrompre le fonctionnement pendant plusieurs jours ou plus observez les pr cautions figurant en page Fehler Textmarke nicht definiert e N utilisez pas l instrument de mani re autre que celles d crites dans ce manuel e Conservez ce manuel proximit de l instrument pour pouvoir le consulter facilement Operating Instructions Page 9 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 1 3 Intended Use For Research Use Only Not for use in diagnostic procedures The device is designed to be operated only be qualified and authorized personnel All users must know the hazards presented by the device and the used substances The fraction collector is designed for laboratory research use in high performance liquid chromatography HPLC and ultra high performance liquid chromatography UHPLC applications The fraction collector is part of the UltiMate 3000 system but can also be used with other systems A control computer with RS 232 or USB port is required The fraction collector is controlled by the Chromeleon Chromatography Management System Observe the following for using the fraction collector e The fraction collector has been designed for the multidimensional separation of biomolecules with aqueous and polar organic mobile
73. r 1 Place the shipping container on the floor and remove any accessories 2 Grasp the fraction collector by the sides Slowly and carefully pull the instrument out of the shipping container and place it on a stable surface A Important The fraction collector weighs more than 8 kg 17 7 lbs Therefore you should use caution when lifting the fraction collector To prevent the unit from falling always lift by the bottom or the sides of the unit Do not lift the fraction collector by the packing material A Important Le collecteur de fraction p se plus de 8 kg 17 7 livres Par cons quent soulevez le collecteur de fraction avec pr caution Afin d emp cher l instrument de tomber saisissez la par le bas ou les c t s de l unit Ne soulevez l instrument l aide du mat riau d emballage 3 The fraction collector is encased in protective wrap and is fitted with form fitting foam spacers which allow for secure placement and positioning inside the shipping box Remove the foam spacers and then remove the protective wrap 4 Before connecting the fraction collector to the power source wait 4 8 hours to allow the instrument to adapt to room temperature and to allow any condensation that might have occurred during shipping to evaporate After 4 8 hours check the fraction collector 1f condensation still exists allow the fraction collector to continue to warm up without connecting it to the power source until any condensation is
74. r advice on the proper handling of a particular substance refer to the Safety Data Sheet SDS of the manufacturer Observe the guidelines of Good Laboratory Practice GLP Dispose of waste substance in an environmentally safe manner that is consistent with all local regulations Do not allow flammable toxic and or infectious substances to accumulate Follow a regulated approved waste disposal program Never dispose of flammable toxic and or infectious substances through the municipal sewage system e Hazardous gases Install the HPLC system in a well ventilated laboratory If the mobile phase or sample includes volatile or flammable solvents do not allow them to enter the workspace If the mobile phase or sample includes volatile or flammable solvents avoid open flames and sparks e Electrostatic discharge Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard This effect is particularly pronounced in insulating capillaries and with non conductive solvents for example pure acetonitrile Take appropriate measures to prevent the generation of static electricity near the HPLC system For example make sure that the air humidity level in the laboratory is sufficiently high and provide proper ventilation wear anti static clothing or shoes prevent accumulation of air bubbles in waste lines and use grounded waste containers Use only non conductive capillaries to direct solvents into the waste contain
75. r version depends on the Chromeleon version e The folder contains the CDM 2 06 00 exe file or a later version Double click the file This will install the drivers Page 36 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector e The folder contains the 2 08 24 folder or a higher number and further sub folders and PDF files The installation instructions PDF file for the drivers can be found in this folder Install the drivers as described in the instructions for your operating system Connecting a USB Cable i Tip Verify that Chromeleon is installed on the computer and that the license code is entered before you connect the fraction collector to the Chromeleon computer To establish a connection via a USB cable 1 Turn on both the Chromeleon computer and the fraction collector 2 Plugone end of the USB cable into the USB port on the Chromeleon computer and the other end to the USB port on the fraction collector i Tips Asan alternative you can connect the fraction collector to the internal USB port on another module in the UltiMate 3000 system that is connected to the Chromeleon computer or connect the fraction collector to the Chromeleon computer via an external USB hub gt Fig 13 page 38 The USB standard limits the USB cable length to 5 meters Each USB device can be separated from the computer or next USB hub by no more than 5 meters 3 The required drivers USB Seri
76. rating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 3 1 Mounting the Diverter Valve Assembly Mounting the diverter valve assembly on the fraction collector arm is the first major task The diverter valve assembly attaches to the fraction collector arm using two knurled finger tight screws 1 Place the diverter valve assembly on the fraction collector arm and mount it with two knurled finger tight screws No tools are required to fasten the screws Fig 3 shows the assembly attached ry Knurled screws Dir Fig 3 Diverter valve assembly 2 Attach the mounting block to the AFC 3000 rear panel with the finger tight mounting block screws provided Fig 4 7 M Fig 4 Attaching the mounting block 3 Connect the diverter valve cable to the auxiliary I O connection on the AFC 3000 rear panel Fig 2 page 18 Tighten the knurled nut finger tight 4 Screw the PEEK drop former finger tight into the diverter valve Operating Instructions Page 27 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 3 2 Diverter Valve Connections When connecting capillaries to the fraction collector observe the following general precautions e Observe the precautionary statements for capillaries and capillary connections in section 1 2 2 page 3 e When you connect capillaries make sure that the connectors are free from contaminants Even minute particles may
77. rating Instructions Page 69 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 70 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 8 Technical Information Tube capacity Depends on rack configuration standard configuration 180 x 8 mL tubes 13 mm x 100 mm Tube change time Typically 0 2 to 0 4 s depending on collect mode and rack type Maximum flow rate 150 mL min Carry over volume 15 uL standard 1 3 uL with optional low flow kit Wetted parts ETFE FFKM Simriz amp PFA PEEK with standard and low flow drop former stainless steel with grounded drop former for normal phase LC Control All functions software controlled via RS 232 or USB requires Chromeleon Chromatography Management System GLP features In Chromeleon All system parameters are recorded in the Chromeleon audit trail Power requirements 100 240 V 47 63 Hz AC 1 9 A with an output of DC 24V maximum 3 33 A Automatic voltage selection Emission sound pressure lt 70 dB A typically 62 dB A level Environmental Range of use Indoor use conditions Temperature 10 to 35 C 50 to 95 F Air humidity 20 70 relative humidity non condensing Overvoltage category II Pollution degree 2 Dimensions h x wxd 43x34x46cm 16 5 x 13 4 x 18 1 in rg 7 Technical information August 2013 All technical specifications are subject to change without notice Operating
78. rformed only by Thermo Fisher Scientific service personnel D Warning D Avertissement The fluid components of the device may be filled with solvents that are harmful to health In the same way harmful substances may be present at the wetted parts Wear appropriate personal protective equipment Rinse the fluid components with an appropriate solvent to remove harmful substances For information about the proper handling of a particular substance and for advice on specific hazards refer to the material safety data sheet for the substance you are using Observe the guidelines of Good Laboratory Practice GLP Les composants fluidiques de l instrument peuvent tre remplis de solvants nocifs De la m me des substances nocives peuvent tre pr sents sur les pi ces en contact avec le fluide Portez l quipement de protection personnel appropri Rincez les composants fluidiques avec un solvant appropri afin d liminer les substances nocives Pour les informations sur la manipulation correcte des substances et des recommandations pour les situations de risque sp cifiques veuillez consulter la fiche de donn es de s curit des substances que vous utilisez Veuillez respecter des directives des Bonnes Pratiques de Laboratoire BPL Operating Instructions Page 61 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Before starting maintenance or service procedures observe the following precautions e For al
79. rincez abondamment la zone affect e l eau puis Page 6 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector e Substances dangereuses De nombreux solvants organiques phases mobiles et chantillons sont nuisibles a la sant Informez vous de propri t s toxicologiques et infectieuses de toutes les substances que vous utilisez Les propri t s toxicologiques et infectieuses de nombreuses substances peuvent tre mal connues Au moindre doute concernant une substance traitez la comme s il contenait une substance potentiellement dangereuse Pour des instructions comment utiliser correctement des compos s particuliers veuillez consulter a la fiche de donn es des s curit s du fabricant respectif Veuillez respecter des directives des Bonnes Pratiques de Laboratoire BPL D barrassez vous de tous les d chets de substances de mani re cologique conform ment la r glementation en vigueur au niveau local Emp chez imp rativement l accumulation de solvants inflammables toxiques et ou infectieux Suivez un programme d limination des d chets r glement et approuv Ne jetez jamais de solvants inflammables toxiques et ou infectieux dans le syst me municipal d vacuation des eaux us es e Gazdangereux Installez le syst me HPLC dans un laboratoire bien ventil Si la phase mobile ou l chantillon contient des solvants volatils ou inflammables vous devez assurer qu ils ne p n tr
80. rueTime 1 00 s PeakStartCurve 1 000 signal s PeakEndCurve 1 000 signal s BaselineDrift 0 000 signal s BaselineOffset 0 000 signal DelayTime 5 5 s To be determined by suitable experiment on site OffsetTime 0 0 s PRK Kok KK AK K AK ck ck KK KK KK RK EK KEKE ok AK AK KA KKK ke ke ck ke KK AK K K AK AKA KKK ke ke e he e e e x Definition of fraction collection driver settings ends here gOCKCKkCKkC KK KK A K AK AK K K KK ko K ko kk ke kk AK A K AK AK KK ke kc ke ke ke heck ko KK ck KK AKA KKK ke e e he e e KEK UV VIS l Wavelength 190 nm Flow 25 000 ml min SB 0 0 isocratic Pump Curve 5 gORKCkCk kk kk ck kc kckckckc kk kk kk kc AK ck AK AK kk ke ke kc kk kk ko EKEKKEKEKKKKEKKKKKKKK KKK Sample run starts here zk kk AK Kk KKK kk ko kc kc kk ko KK kk kk ck kk ko ke ke hec he KK kk ck kk kk ke ke e ke ke ke KKK 0 000 10 000 10 142 Autozero Wait UV Ready and Sampler Ready Inject Pump Pressure AcqOn CollectFractions By Peak CollectOutsidePeaks No UV VIS 1 AcqOn Pump Pressure AcqOff CollectFractions No UV VIS l Acqoff AFC Valve Drain Close the diverter valve after a run to avoid accidental spillages End End time is crooked because of internal processing times required by triggers Operating Instructions Page 79 UltiMate 3000 Series AFC 3000 Automated Fraction Collector pOCKCKCKCk kk kk kk kk k
81. s in the Commands F8 dialog box Direct commands are executed as soon as they are entered For routine operation most parameters and commands are also available on a control panel To open the Commands dialog box l Open a control panel any panel is possible To open a control panel open the Chromeleon Browser and double click a control panel in the Dionex Templates Panels folder Connect the control panel to the timebase in which the fraction collector is installed On the Control menu select Connect to Timebase and then select the timebase on the Timebase tab For information about the Timebase dialog click Help i Tip The Control menu is visible only when a control panel is already open Press F8 or click Command on the Control menu To view the available parameters and commands for the fraction collector click the plus sign beside AFC The commands and parameters available in the dialog box may vary depending on the Chromeleon version and the display filter level Normal Advanced or Expert Page 46 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 Change the display filter level if necessary Right click in the commands list and select the filter level on the menu Commands PC1_UltiMate3000 93 Rack 93 MoveTo suapte EISE 9 RinsePosition Y POUPO OYES TISA iB ModelNo Normal D I CollectMode Advanced MovementMoc Expert Valve D I TubePosition
82. s of the fraction collector for power connections and ventilation e Make sure the unit is not exposed to excessive flammable or corrosive materials e Protect the fraction collector from long term exposure to condensation corrosive materials solvent vapor continual standing liquids or large spills into the fraction collector cabinet or fraction collector arm Exposures of this type can damage the drive mechanisms as well as the electronics Operating Instructions Page 23 UltiMate 3000 Series AFC 3000 Automated Fraction Collector e Observe the same general electrostatic discharge precautions as with any other integrated circuit electronic devices Low humidity environments especially when combined with static generating materials require maximum care A Important Discharge static buildup and ground to the fraction collector base or cabinet before performing any maintenance Do not touch or short circuit bare contacts for example COMI or auxiliary ports A Important D chargez accumulation d lectricit statique et mettez la terre la base et le cabinet du collecteur de fractions avant de proc der tous travaux d entretien Ne touchez ou court circuitez pas des contacts nus par exemple COMI ou ports auxiliaires e Avoid using the AFC 3000 if strong electromagnetic interference radio frequency interference or radioactivity is present Interference fields can cause erratic operation of the fraction collector Th
83. s that are within the allowed range Internal error required Verify that you have defined a legal rack type for all used rack parameter was missing positions and NotUsed for unused rack positions page 49 Invalid enumerated value 3 Verify that you have entered valid rack types only page 49 Parameter TubePosition value You have entered a tube position that exceeds the number of 384 exceeded its allowed range Set to Enter a valid tube position This error appears only with the use closest valid value 384 of the 96Pos rack type Page 60 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 7 Service This chapter explains how to clean the AFC 3000 inspect it for leaks and replace damaged components A Important A Important Discharge static buildup and ground to the fraction collector base or cabinet before performing any maintenance Do not touch or short circuit bare contacts for example COMI or auxiliary ports D chargez accumulation d lectricit statique et mettez la terre la base et le cabinet du collecteur de fractions avant de proc der tous travaux d entretien Ne touchez ou court circuitez pas des contacts nus par exemple COMI ou ports auxiliaires 7 1 General Notes and Safety Precautions The following sections describe all procedures for the fraction collector that the user may perform All other maintenance and service procedures must be pe
84. se includes the Fraction Collection license Rack 21 position 50 mL 30 mm x 100 mm 6702 0021 Rak poston 2 m m 10 mm Joer Rack 40 position 20 mL 20 mm x 100 mm 6702 0040 Rick 6 postion am 16m om 87024080 eo Adapter for WPS racks 6702 0100 Required for use of all WPS racks with the AFC 3000 Rack 36 position Foxy scintillation vials 5701 2025 28 mm OD Rack 60 position Foxy 1 5 mL 5701 2023 E pan be USO Tor oxy TUIS T fraction collectors Rack 72 position Foxy mini tubes 18 mm 5701 2024 OD Positioning pins for Foxy racks 6702 9006 Pins facilitate installation of Foxy racks on the AFC 3000 Adapter for 4 well plates 6702 0200 An adapter is required for using well lat ith the AFC 3000 Adapter for 2 well plates Foxy Jr R1 5701 2021 OS E Funnel rack 6702 1021 To be used in conjunction with the 21 position rack 6702 0021 Tubing included Page 74 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 9 3 Consumables and Spare Parts The part number always refers to the packing unit Unless otherwise stated the packing unit is unit For more information contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products Description Part No 24 V external power supply 6702 9010 Bottle 0 25 L 2270 0026 Capillary markers 10 pcs 6040 2399 Diverter valve 0 4 mm ID drop former and tubing Kit for low flow rates 670
85. shthe open clip end of the grounding cable from the normal phase kit between the diverter valve and the drop former Fig 25 Fig 25 Installing the normal phase ground strip Page 64 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 5 Remove the ground cable screw and reinstall with the normal phase grounding cable attached Make sure to lead cable along the front side of the diverter valve assembly Fig 26 Ground cable screw Fig 26 Normal phase ground strip i Tip The carry over volume of the normal phase kit is the same as for the standard configuration Operating Instructions Page 65 UltiMate 3000 Series AFC 3000 Automated Fraction Collector 7 5 Installing the Kit for Low Flow Rates The kit for low flow rates includes a tool for installation and a piece of 0 3 mm ID PTFE tubing Before you start make sure that you have all parts of the kit at hand 1 Shut down and unplug the fraction collector Set the pump flow to 0 2 Dismount the current diverter valve from the assembly page 67 3 Connect the diverter valve cable to the auxiliary I O connection on the AFC 3000 rear panel Fig 2 page 18 Tighten the knurled nut finger tight 4 Remove the two valve screws that are attached to the new diverter valve 5 Remove the drop former and drop former screw from their packaging and insert the drop former into the screw Fig 27 Then place
86. sition Fracs5tatt Collect AFC TubePosition TubeChange Drain Collect AFC TubePosition FracEnd Drain AFC TubePosition PRK K K kk AK kk KA KK KK KK ko kk ke ck ck AZ KAZ KZ ke kc KKK ke kk ke ke kk kk ck ck kc kc kc kc kk e KK Definition of triggers for fraction collection ends here gOCKCKCKCKCkUK kk KK AK AZ KK KK KK KK kc kk kc kk koc c KAZ kc kc kc kc kk kc kc kk kk kk kk ck kk kc kc kc kc kc k ke kk PRK K K kk AK AK KZ KK KK kk ke KK ck ck AK kc kc kc KA ke ke ke ke ko kk ke kk ck ck ck kc kc kc kc ke ke e ke x Definition of fraction collection driver settings starts here gORCKCKCKCKkCkk kk kck ck AK KA kc ko KK kk KA KK KK AK ck AK KAZ K kc KK kc kc ko kk ke kk ck ck ck kc kc ke ke ke ke e e KE FractionCollection TubeMaxVolume TotalNumberInstalle MaxTubesPerFraction TubeWrapping TubeChangeDuration DetectionChannell N PumpDevice d ane Pump 40 000000 42 10 No 0 5 Fs ml UV VIS 1 Page 18 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector PeakStartThreshold 10 00 signal PeakStartSlope 0 500 signal s PeakStartTrueTime 1 00 s PeakEndThreshold 9 00 signal PeakEndSlope 1 000 signal s PeakEndTrueTime 1 00 s DerivStep 1 00 s ThresholdNoPeakEnd 2000 00 signal ThresholdDoNotResolve Off PeakMaxSlope Off PeakMaxT
87. st utilis de fa on non sp cifi e par Thermo Fisher Scientific la protection pr vue par l instrument pourrait tre alt r e Thermo Fisher Scientific n assume aucune responsabilit et ne sera pas responsable des blessures de l operateur et ou des dommages de l instrument Si la protection de l instrument n est pas garanti tout moment d branchez l instrument de toutes les sources d alimentation lectrique et assurez vous que l instrument n est pas utilis involontairement 1 4 Federal Communications Commission FCC Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his expense Operating Instructions Page 11 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 12 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 2 Overview 2 1 Unit Description The fraction collector ca
88. stalling funnel racks on the 21 position rack connect tubing shipped with the rack to each funnel and route the tubing to an appropriate container or bottle Observe the instructions for drain tubing page 29 Depending on the rack type you can install a certain number of racks deep well plates or bottles The maximum number of racks and the order in which the racks must be placed and configured in Chromeleon can be taken from table on page 31 The rack layout is shown facing the front of the unit Note that it is not allowed to install a rack for example in position 2 if no rack is installed in position 1 For details on setting the racks in Chromeleon refer to section 5 2 3 page 49 Fraction rack Maximum number of racks bottles Rack layout 21Pos 24Pos 40Pos 60Pos 90Pos Two racks can be installed on the universal drip tray holding the specified number of tubes Any two racks may be used together at one time mmm mm Se min min e Ae M JJ a a Din in D A ba ep nt Jon De AAA AA hmm mmm mm es e n Ton Den en en en n m t eh jn jet e Jan AURA AAA AAA mmm RRP RP KEN KKH on the Foxy well plate holder 36Pos Foxy One Foxy rack can be installed on the S 4 2 60Pos Foxy universal drip tray holding the specified D f 4 72Pos Foxy number of tubes H f L i dire DH LEL 96Pos_Foxy Two well plates 96 positions can be installed Operating In
89. structions Page 31 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Fraction rack Maximum number of racks bottles Rack layout 96Pos Four well plates 96 positions can be installed E on the well plate adapter Well plates must not be mixed with other rack types RATS Get Rackt Rack2 10Pos WPS Four WPS 3000 well plate sampler racks can 22Pos WPS be installed on the rack adapter Any four 40Pos WPS racks may be used together at one time Bottle Six 250 mL Schott bottles can be placed directly on the universal drip tray You must always install all six bottles It is not possible to mix bottles with other rack types Foxy rack positioning pins When Foxy Jr R1 racks are used Thermo Fisher Scientific recommends installing two positioning pins part no 6702 9006 to prevent incorrect installation and orientation of a Foxy rack Place the pins in the holes provided in the universal drip tray With the pins installed a Foxy rack cannot be installed incorrectly on the universal drip tray Fig 10 Positioning pins for Foxy racks Positioning pins Page 32 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector i Tip To prevent the universal drip tray from accidentally being lifted out of its proper position always remove the rack adapter toward the side as shown here Fig 11 Removing the rack adapter 3 3 6 Adjusting the Dro
90. ted recesses in the tray Fig 9 Other rack types such as Thermo Scientific Dionex well plate sampler racks and the 4 x 96 well plates require special adapters which can be ordered separately The adapter block 1s correctly installed on the drip tray in one position only when the recess on the bottom of the adapter block is properly aligned with the elevated parts of the drip tray KS E Universal drip tray CE CE Rack L CE L CE Fig 9 Racks placed in universal drip tray Page 30 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector AN Important A Important Before loading or unloading any fraction racks on the universal drip tray move the arm with the drop former to the home position by cycling the power off and on or by sending the RinsePosition comand Never attempt to load unload or reposition a fraction rack or fraction tube while the fraction collector is operating Avant de charger ou d charger aucun des racks de tubes de fraction du plateau de fractions parquez le bras avec le g n rateur des gouttes en position d origine par eteindre et allumer cons cutivement l instrument ou donner la RinsePosition commande Jamais tentez charger d charger ou repositionner un des racks de tubes de fraction ou un tube de fraction tandis que le collecteur de fraction est en operation When in
91. to avoid crashes section 3 3 6 page 33 and section 4 page 34 3 3 4 Connecting the Drain Tubing The rinse station drain tubing attaches to the rinse station Use an elbow piece shipped with the fraction collector to connect the drain tubing to the rinse station Fig 7 Connect another drain tubing in the same way to the integrated drain at the front left corner of the drip tray You can use the Y piece shipped with the fraction collector to connect the two drain tubes to a single drain You can also connect them to an UltiMate 3000 drain system if applicable The tubing should be routed to an appropriate waste container or drainage system Tray drain Rinse station Elbow piece Drain tubing P SH Fig 7 Connection of the drain tubing Place the waste container below the fraction collector to allow any liquids to flow off Ensure that the free end of the tubing inside the waste container is always above the liquid level If necessary shorten the tubing Operating Instructions Page 29 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Fig 8 Rinse station tubing in waste container 3 3 5 Placing Fraction Racks on the Universal Drip Tray The fraction tube racks in the AFC 3000 accessories kit are shipped unassembled First assemble the racks using the supplied instructions Then position them on the universal drip tray using care to line up the rack locating pins with the associa
92. tomatic sample purification via the associated post acquisition steps color coded sample and fraction tracking and sophisticated fractionation algorithms with peak shoulder detection For more information about the Chromeleon licenses contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products Two modes of software control are available Direct Control With direct control you select operating parameters and commands in the Chromeleon Commands F8 dialog box Direct commands are executed as soon as they are entered For routine operation most parameters and commands are available also on a control panel For more information about direct control see page 46 Automated Control With automated control you use a program file This is a list of control commands executed in chronological order for automated operation of the fraction collector For more information about automated control see page 52 Operating Instructions Page 21 UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 22 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 Installation 3 1 Facility Requirements The AFC 3000 operates reliably even under less than ideal conditions It is not however indestructible Malfunction or damage can occur if specific operating conditions are not met Meeting these conditions requires that you create a proper lab environment replace fraction
93. wever ensure that your lab environment provides operating conditions that will prolong the life of the AFC 3000 When you finish using the fraction collector you may need to flush the rinse station and flow path before shutting the fraction collector down The AFC 3000 can be operated with the Chromeleon Chromatography Management System Stand alone operation is not supported 5 1 Power Up Once you arrange the tube racks and ensure that the arrangement is correctly defined in the software you can start the fraction collector and let it run until the sampling sequence is finished To do so complete the following steps 1 Ensure that the rinse station is properly connected section 3 3 4 page 29 2 Turnthe AFC 3000 power switch on The blue LED indicator along the fraction collector x axis lights up when the power is on 3 Ensure the waste tubing is in the correct waste container and proper drain flow is achieved 4 Connect the AFC 3000 to Chromeleon section 5 2 1 5 2 Operation with Chromeleon Before you begin verify that 1 The Chromeleon software is installed on the computer and the license code is entered The minimum requirement for control of the AFC 3000 is Chromeleon 6 80 SR7e Driver Update If a USB cable is used for communication a USB converter software must be installed in addition page 37 2 The fraction collector is connected to the Chromeleon computer 3 The fraction collector is set up in
94. xample Page 38 Operating Instructions UltiMate 3000 Series AFC 3000 Automated Fraction Collector 3 5 Setting Up the Fraction Collector in Chromeleon This section provides brief instructions for installing and configuring the fraction collector in Chromeleon i Tip The minimum requirement for control of the AFC 3000 is Chromeleon 6 80 SR7e Driver Update 3 5 1 Installing the Fraction Collector l Start the Chromeleon Server Monitor program by double clicking the Chromeleon Server Monitor icon a5 on the Windows taskbar If the Server Monitor icon is not on the taskbar click Start on the taskbar point to Programs or All Programs depending on the operating system point to Chromeleon and then click Server Monitor Click Start to start the server Click Close to close the Chromeleon Server Monitor window The Server Monitor icon e appears on the taskbar i Tip Clicking the Quit Monitor button in the Chromeleon Server Monitor window quits exits the Server Monitor program but does not stop the server To stop the server click Stop Start the Chromeleon Server Configuration program by clicking Start on the taskbar Point to Programs or All Programs depending on the operating system point to Chromeleon and then click Server Configuration If necessary click the plus sign beside the server icon S to display the items underneath Select the timebase to which the fraction collector will be assi

Download Pdf Manuals

Related Search

Related Contents

Poulan Pro PP025 Trimmer User Manual  Melissa 635-072 User's Manual  Poinçon de rayure d`après Sikkens Modèle 463  Sun Blade 6000 Disk Module Service Manual  12A - PF photon factory  Bintec-elmeg S560  R&S FSW-K7 Analog Demodulation User Manual  Integral IN3T4GEZBIXK2 memory module    Severin KS9872 fridge-freezer  

Copyright © All rights reserved. Failed to retrieve file