Leica EG 1160
Contents
1. 0386 12303 DAK 25 9 DIT 0386 12304 902 24 2 9 MIN riae ameet RR uL EE MERO 0386 12305 91 25 X 5 MI sacs cse sussumc 0386 12306 Embedding molds deep version for samples up to 24 x 24 x 12 mm eene 0386 12860 912524 12 0386 12961 Marker pencil for marking of cassettes and embedding rings ccccsccseessessssssssssesessesessnsesenseesnseesessesesensenees 0386 12300 Filing box for convenient storage of cassettes lids molds and embedding rings 0394 12398 Plastic storage cabinet with 4 drawers for cassettes OF rings 0386 12393 Embedding frames size 1 56 x 40 x 26 mm adjustable eene 0339 06427 Embedding frames size 2 34 x 26 x 18 mm adjustable seen nnne 0339 06432 Base plate for Catalog No 0339 06427 or Catalog No 0339 06432 0339 06438 Paraflex embedding mold for 8 samples 25 x 25 x 25 mm each senes 0339 10963 Paraflex embedding mold for 4 samples 40 x 40 x 30 mm each 0339 10961 Embedding mold for plastics flexible but stable in form 8 recesses 25 4 of which with gate for specimen2. goo EE LN Leica EG 1160 Paraffin Embedding Center Instruction manual Leica EG1160V4 1 English 05 2001 Always keep this manual near the instrument Read carefully prior to operating the instrument MICROSYSTEMS 1 Important information Serial No Year of manufacture Manufactured in Federal Republic of Germany CLCCL MICROSYSTEMS Leica Microsystems Nussloch GmbH Heidelberger Strasse 17 19 D 69226 Nussloch Germany Telephone 062 24 143 0 Telefax 0 62 24 143 200 eMail histo_info leica microsystems com Internet http www histo solutions com Leica EG 1160 Paraffin Embedding Center The information numerical data notes and value judg ments contained in this manual represent the current state of scientific knowledge and state of the art tech nology as we understand It following thorough investi gation in this field We are under no obligation to up date the present manual periodically and on an ongo ing basis according to the latest technical develop ments nor to provide our customers with additional copies updates etc of this manual For erroneous statements drawings technical illus trations etc contained in this manual we exclude li ability as far as permissible according to the national legal system applicable in each individual case In par ticular no liability whatsoever is accepted for any fi nancial loss or conseq
3. 0502 30213 tbe iret bitte t edocti 0502 30214 290 V 50 600 Z 0502 30215 50 COZ ncs o pau EE het Dna 0502 30216 If you need further accessories please contact your Leica sales office or dealer using above ordering information Leica EG 1160 Paraffin Embedding Center 11 8 Components and features Paraffin reservoir 13 The paraffin reservoir can hold up to 3 liters The paraffin temperature can be adjusted in a range from 45 to 70 C The reservoir has an overheat pro tection in case of a temperature controlfailure The paraffin quantity level is indicated in the display graphically both in the standby and operating con dition The display indicates two levels 1 Reservoir filling quantity 11 31 2 Reserve max 1 When the reserve diagram is indicated there is still approximately 1 liter in the reservoir so it is not necessary to interrupt work immediately Never theless it is necessary to refill the paraffin reservoir as soon as possible A grid inside the paraffin reservoir provides that only molten paraffin flows through the dispenser outlet nozzle to ensure troublefree embedding An integrated filter at the junction point of dispenser and reservoir prevents any contamination of molten paraffin Each refill of the reservoir can cause the formation of air bubbles that im pede a smooth paraffin flow This can be overcome by pushing the paraffin dispenser handle
4. C aM EI 5 echnical ad opened aaa 6 deseriplioli ee 7 Iain EC 8 Leica EG 1160 DVOEVIGW icis aisi d E RE A Fu EL E FUE CU CR Tear lu sa pa FOEDE ICE MCI AE 10 Components and features nitet ect t P va fudit tu esa 12 Controls 7 Display tpe nod Sa estes scent Gu ed En MM EE 16 Operation 17 Tissue emiedding 24 Cleaning maintenance Service 26 POU PEG SH OOUIIG 28 Ordering informatii etica ite incon bc idc bc b Bc Pc Rb C 31 Warrenty and SOU VIG nO Bec on ev n 32 Instruction manual V4 1 05 2001 3 Installation The Leica EG 1160 is a compact bench top unit and can be set up easily All components come in a cardboard box The components that are packed separately can be attached to the instrument easily see page 5 Part list 1 Transport and assembly position The Leica EG 1160 must never not even for a short while be positioned as shown These positions will inevitably cause damage to the condenser Therefore please check the Tip N Tell indicators which are attached to the package upon receipt Shoul
5. Heater failure ACTION Technical service Technical service Activate heater paraffin reservoir Technical service Replace lamp Push dispenser handle for a while until flow is homogeneous Technical service Check temperature setting Activate heater Technical service Check if lid is properly oppositioned Check seal Check knob adjustment Technical service Technical service 29 13 Troubleshooting FUNCTIONAL UNIT SYMPTOM CAUSE ACTION Cold plate Insufficient cooling Ambient temperature too Check temperature high atlower ambient temperature Leak in cooling system Technical service Air supply to compressor Clean condenser fins insufficient Technical service Compressor does not Compressor failure Technical service start Compressor stops after Compressor failure Technical service short operation Compressor stops after Compressor failure Technical service short operation and restarts after a short while Telephone number of your local sales office MICROSYSTEMS 30 Instruction manual V4 1 05 2001 Leica EG 1160 Paraffin embedding center 14 Ordering information Catalog Number Basic instrument 115 VIGO AZ 0386 30527 0386 30528 Model with additional forceps holder on the left 115 V 60 Hz 0386 30351 endroit end oid RA vato erp eed ne vut bac 0386 30352 Additional cassette Dat adeoteeanidaiodiatetr eed
6. programming operation some of the push buttons have mul tiple functions initialisation ACT Mode SET Mode Operation Programming Standby a Deactivation or reactiv ation of the previously ON n selected temperature OFF To turn instrument ON To turn instrument ACT mode ON OFF status line default reading b Programming of the workdays Language selection Adjustment of the setting of variable only after turning on paraffin flow rate parameters temperature with the main switch day time on the back SET ACT mode gt SET mode Illumination Illumination ON OFF ON OFF scrolling through scrolling through the menu the menu For details on operation and programming please refer to chapter 10 Operation 16 Instruction manual V4 1 05 2001 10 Operation Please ensure that all preparatory steps described in chapter 11 have been completed 1 Putting the instrument into operation after delivery Turn the instrument on with the main switch green toggle switch in the lower right corner on the back of the instrument while pushing the ARROW UP button on the control panel for approx 2 seconds Then push the ON OFF button The instrument will then be initialised The main switch should always remain in ON position even after daily work In daily routine the tissue embedding center is turned on off by pushing the ON OFF button on the control panel Turning on agai
7. cassette bath which is a standard accessory The vacuum lid allows for vacuum infiltration of the tissue while stored in the wax bath that means com plete infiltration of the tissue in less time The vacuum pump not included is connected to the connecting branch 27 Care should be taken that the lid 26 is seated flat on the cassette bath The seal of the lid must be free of paraffin to ensure tightness so that a vacuum can build up To improve the tightness the lid should be pushed on to the cassette bath when the vacuum pump starts to oper ate A vacuum can be generated only if the aeration knob 28 is shut After infiltration the lid cannotbe opened immediately First the knob 28 has to be opened slowly to allow normal pressure to establish inside Note After infiltration the vacuum should be neutralised very slowly as air entering too rapidly could cause hot paraffin to splash and therefore should be avoided Fiber optical light guide for connection to the coldlight sources of Leica CLS series To optimally illuminate the dispensing area a fiber optical light guide with a cold light source can be used Leica EG1160 Paraffin Embedding Center 15 9 Controls Display Control panel 1 The control panel with push buttons and the 2 line display 2 is protected with a PE foil Leica EG 1160 SC OFF Set ET OFF a Push button functions Depending on the mode of operation initialisation
8. low Heater not activated Wrong temperature selected Instrument failure Paraffin not entirely molten Foot pedal not properly connected ACTION Turn on main switch Hold down ON OFF button for some seconds Wait until the wax Is molten completely then check again Increase temperature of paraffin reservoir Check if paraffin reservoir heater Is activated Check temperature setting and activation Technical service Check the consistency of the wax in the reser voir and wait for some time If necessary Check foot pedal connection Instruction manual V4 1 05 2001 13 Troubleshooting FUNCTIONAL UNIT Paraffin dispenser Mold warmer Cassette bath Work area Vacuum attachment tional accessory Forceps holder SYMPTOM No paraffin flow Paraffin drops when valve closed No heating No illumination Inhomogeneous paraffin flow e g air bubbles Wrong temperature indication No heating No vacuum buildup Vacuum deteriorates too rapidly No heating Leica EG 1160 Paraffin Embedding Center CAUSE Foot pedal dispenser valve switch failure Valve pump failures Heater paraffin reservoir not activated Heater failure Lamp defective Air bubbles in dispenser at the start Pump failure Wrong temperature selected Heater not activated Heater failure Lid not properly positioned Seal of lid contaminated Aeration knob open Seal untight
9. orientation 19 mm deep enn 0379 12698 Embedding medium JUNG Histowax 1 bag 2 5 0374 08585 Largesbagi259 0374 14374 Leica EG 1160 Paraffin Embedding Center 31 15 Warrenty and service Warranty Leica Microsystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control procedure based on the Leica in house testing standards and thatthe productis faultless and complies with all technical specifications and or agreed characteristics warranted The scope of the warranty is based on the content of the concluded agreement The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively Technical service information If you require technical service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information Model name and serial number of instrument Location of the instrument and name of the person to contact Reason for the service call Date of delivery Decommissioning and disposal The instrument or parts of the instrument must be disposed of in compliance with the local laws 32 Instruction manual V4 1 05 2001
10. until a smooth flow is achieved The paraffin flow rate is adjustable in 10 increments see nitial operation Controls A pump ensures a smooth and precisely controlled paraffin flow Due to Its special design the pump 15 protected against damage by solid or semiliquid paraffin Paraffin dispenser with illumination 8 The dispenser is separately heated and always has the same temperature as the paraffin reservoir The dispenser handle 9 is used for manually op erating the paraffin flow and is provided with a dispenser handle extension clip 29 The paraffin flow is released by lightly pushing the mold backward against the extension clip of the dispenser handle thus opening the dis penser valve and activating the pump Releasing the handle a spring causes Itto return to the start position Thus the valve is shut and the pump opera tion Is stopped When dispensing is controlled with the foot pedal the dis penser handle is not needed and can be flipped back out of the way This provides ample space allowing convenient filling of larger molds The homogeneous diffuse illumination of the embedding area and refrig eration spot provides an optimal view of the embedding process and speci men orientation The light bulb can be removed after removing the cap 23 12 LLL Instruction manual V4 1 05 2001 8 Components and features Mold warmer 14 The temperature of the mold warmer is adjustable fr
11. vacuum attachment please ensure that af ter infiltration the vacuum is neutralised very slowly Therefore open the aeration knob very cautiously Air entering too rapidly could cause hot paraffin to splash and should therefore be avoided Priorto connecting the instrumentto the power supply in the labora tory please make sure that the power supply complies with the val ues specified on the nameplate The tissue embedding center should be connected to a grounded socket only The equipment mustnotbe setup near an air condition vent or should not be exposed to direct sunlight Fullrefrigeration capacity is ensured only ifthe clearance on the back Qa of the instrument is not less than 15 cm Therefore itis necessary to mount the spacers provided Prior to any maintenance and service action the tissue embedding center must be disconnected from the power supply Leica 1160 Paraffin Embedding Center 9 1 Leica EG 1160 Overview Illumination button 3 LCD display 2 Leica EG 1160 p OFF Menu button 4 Arrow UP DOWN ON OFF button 7 buttons 6 SET ACT button 5 Conto panel 1 Dispenser with integrated illumination 8 Forceps holder 11a only on model with additional forceps holder on the left Paraffin reservoir 13 Mold warmer 14 Cover cap for illumination 23 Cold plate 17 Dispenser handle extension clip 29 Refrigeratio
12. H ALLY elect the desired language within 10 15 seconds Sel he d di hin 10 15 d Standby MO 12 30 LLL Status line ACT on SET OFF SET 10sec PAR TANK XX C 4 SET TEMPERATURES 10 sec Status line SHEE PAR TANK XX C UU OS 10sec COLD XX C AI SET TEMPERATURES 10 sec Status line TTT COLD PLAT XX C 10sec f CASS BATH SET SET TEMPERATURES 10 sec Status line BEEN 4 gt CASS BATH XX C 9 WU MOLD TRAY XX C SET SET TEMPERATURES 10 sec Status line SEES 4 MOLD TRAY XX C LU O ee 10 WORK SURF a SET TEMPERATURES 10 sec Status line TIT WORK SURF XX C 9 HOO TODAY 15 10 sec MO vm JO THE TIME IS 10 sec A 12 30 HRS qu X Illumination ON OFF ry n O START WORK AT 10 sec ON 10 00 HRS ON OFF Standb or ON Standby emo STOP WORK AT 10 sec 16 00 HRS WORKDAYS 10 sec MO YES AIL 0 HA 1009 SET SET SET FLOW RATE 10 ACT AJUST ACT Status line NEOWRATE 10 10 sec Illumination ON OFF Activation of temperature control OFF Activation of workdays Leica EG 1160 Paraffin Embedding Center 23 11 Tissue embedding The paraffin reservoir is filled with wax pellets e g Leica HISTO WAX The level of the molten wax should reach up to 2 3 cm under the upper edge of the reservoir When the symbol for paraffin tank on reserve 15 displayed there
13. araffin can drain to the tray and prevent the removable tray from sticking to guide 5 Illumination of the dispenser outlet Prior to exchanging the light bulb the Leica EG 1160 must be turned off with the green main switch on the back Remove the cap atthe front end ofthe dispenser The light bulb can be removed pulling it toward the user The replacement bulb is inserted by lightly pushing it between the con tacts 6 Fuses See Technical data Replacement fuses must meet the specifications of the manufacturer Otherwise this could invalidate the warranty Leica EG1160 Paraffin Embedding Center 27 13 Troubleshooting Whenever a malfunction occurs please ensure that It is not the result of an operating mistake The list below includes the most frequent operating errors Equipment malfunctions are indicated as error messages error codes 1 to 13 in the LCD display If such an error code is displayed please contact your local Leica service center FUNCTIONAL UNIT Initial operation Paraffin reservoir Paraffin dispenser 28 SYMPTOM Instrument does not turn on Paraffin quantity level indic ation does not correspond to the actual quantity Paraffin does not melt Temperature readout Incorrect or no heating No paraffin flow CAUSE Main switch on the back notin ON position ON OFF button pushed long enough Paraffin not entirely molten selected temperature too
14. d it become necessary to bring the instrument in a position other than normal lay the instrument on its back turning it slowly and carefully 2 Unpacking The instrument may only be lifted holding it at the sides of the base plate of the housing Dot not carry the instrument holding it at the top cover atthe front handholds of the removable trays or the dispenser Risk of fracture 3 Place of installation The instrument should be set up on a stable plane laboratory table Please ensure that there Is no air condition vent nearby The location must fulfil the following conditions to ensure that air circulation is not affected The back of the instrument must be atleast 15 cm away from 15 the wall shipmentincludes two spacers which must be attached to the E back Non compliance with this minimum distance can cause substantial damage to the instrument and will invalidate the warranty 4 Power supply Prior to connecting the instrument to the mains outlet please ensure that the power supply specified on the nameplate complies with the local mains conditions 5 Foot pedal The foot pedal is connected to the socket in the right hand bottom corner on the back Leica EG1160 Paraffin Embedding Center 5 4 Technical data Power supply 115V 60Hz Dimensions Programmable parameters 230V 50Hz LxDxH 910 x 575 x 320 mm Temperature Fuses Weight 50 kg Paraffin reservoir 115V 60Hz F1 F2 T5 0A Mold war
15. e indicated day will be put in the memory automatically The ON MO OFF button is used to confirm if the program shall be activated YES or not NO the day indicated in the display ON OFF If the programming of workdays is not desired i e if the user can do entirely without the automatic turn on function of the tissue embedding oo NI center NO must be entered for each day of the week 6 5 How to quit the programming SET mode 1 10 seconds after activation of any of the buttons the in T PARTUM DO strument will automatically return to the ACT mode de PARTANK XX C SEC rU 151 NEEE fault reading This feature is a protection against unintended program ming ET 2 the SET ACT button will also lead to the ACT E mode The flow rate cannot be selected in the SET mode It can only be selected in the ACT mode AUTOMATIC TURN ON FEATURE If this feature is not used it should be ensured that the tissue embed ding center is turned on early enough to allow all components to reach the appropriate temperatures to avoid unnecessary delays Especially the paraffin reservoir heater should be turned on early enough as it can take some hours until the wax is molten The indi vidual warmup times are listed in chapter 4 Technical data 22 Instruction manual V4 1 05 2001 Y Flow chart Main switch 4 4 2 sec ON OFF Self test SPEAK ENGLIS
16. e inte grated in the instrument The heated recessed area behind the dispenser handle is designed to keep the par Leica EG1160 Paraffin Embedding Center affin in the cassette molten Excess paraffin can be drained through an outlet after the removing the stopper In frontofthe paraffin dispenser out let a refrigeration spot is integrated in the cold plate ensuring optimal cooling The large cold plate pro vides space for more than 60 embed ding molds A removable tray is lo cated under the work area to collect excess paraffin drained from the work area The Leica EG 1160 is operated and programmed via a menu driven con trol board The menu is displayed in German English French Spanish or Italian as selected All operations are microprocessor controlled Malfunctions are indicated by a nu merical error code in the display A battery backup prevents loss of all programmed parameters if a power failure occurs The individual functional units of the Leica EG 1160 are ergonomically po sitioned to enable rapid and conve nient operation The instrument may be operated by qualified personnel only in accordance with this operat ing Instruction manual If used in accordance with the op erating instructions and appropriate maintenance is ensured the tissue embedding center will operate trouble free and give perfect embed ding results for many years 6 Safety precautions instrument may be used b
17. e micro tome 8 Place the mold on the cold plate The wax will become entirely solid within a short time Finally the par affin block including the specimen can be easily removed from the mold All steps should be performed with out delays to ensure that the wax homogeneously solidifies in the mold to prevent the formation of layers which could affect the sectioning results Please note that the removable tray where excess paraffin is drained should be emptied at regular inter vals to ensure that liquid paraffin drains easily and the removable tray does not stick to the guides Caution The tray can be hot Excess paraffin collected in the re movable tray should not be reused as it would be contaminated 9 On termination of embedding the Leica EG 1160 should be turned off pushing the ON OFF button Conden sation water on the cold plate should be wiped off and liquid paraffin re moved from the work area with soak ing paper For further information on cleaning and maintenance please refer to chapter 12 Note The tissue embedding center need notbe turned off with the green main switch on the back If it is pro grammed to automatically turn on in advance it must not be switched off with the main switch Leica EG1160 Paraffin Embedding Center When the automatic turn on feature Is not used please make sure that the instrument is turned on early enough to allow the individual com ponents to reach the
18. ination 8 User menu available in 5 lan guages 9 Fault detection by error codes in the display 10 Optional accessories Magni fier vacuum attachment fiber optical light guide for connec tionto the coldlightsouces ofthe Leica CLS series The Leica EG 1160 is a modern tis sue embedding center providing all features required for rapid conve nient and efficient paraffin embed ding of tissue including a separately heated 3 liter capacity paraffin res ervoir Paraffin quantity level shown on LCD display in two steps Sepa rately heated paraffin delivery sys tem with integrated filter and pump ensure a smooth consistent paraf fin flow The paraffin flow rate is ad justable in 10 increments The paraffin outlet nozzle can be op erated either manually or via foot pedal non glare illumination is in tegrated at the paraffin dispenser outlet to provide a well lighted work area The separately heated mold warmer to the right of the paraffin reservoir Is designed to store and heat the molds prior to embedding The work area is divided into three parts Cassette storage working and embedding area and cold plate The cassettes containing the tissue are stored in a separately heated re movable tray with a capacity of approx 100 specimen capsules Thelarge work area ensures conve nient block removal and easy access to the cassettes The forceps hol der s is are designed to accommo date three forceps and is ar
19. ing mode SET mode Note If a flow rate of 10 is selected the SET ACT button will lead to the programming mode ADJUST FLOW RATE see 5 3 PAR TANK ON TU 13 15 LLL 10 1315 LEL 5 5 SWITCHING OFF with the ON OFF button By pushing the ON OFF button the instrument au tomatically returns to the standby condition Itis not necessary to switch the tissue embedding center off with the main switch If switched off with the main switch all programmed parameters are maintained In this case however the program will not be activated In the SET mode the ON OFF button has an addi tional function for the programming of temp eratures and workdays see 6 1 6 4 Leica EG 1160 Paraffin Embedding Center 19 10 Operation SET Mode Programming 6 The SET mode is activated by pushing the SET ACT button The SET mode is used for programming only The programmable parameters are as fol SET lows ACT Paraffin reservoir temperature Cold plate temperature Cassette bath temperature Mold warmer temperature Work area temperature Actual day Actualtime Planned start time Planned finishing time Workdays The instrumentis programmed to have all functional units ready to oper ate at the preselected time on the preselected workdays i e all heated and refrigerated components are turned on automatically so that they will have reached the preselected temperatures whe
20. ing to work the cold plate should be free of paraffin and dry to prevent frost buildup Paraffin collecting tray 18 A large removable tray is located under the heated work area to collect excess paraffin drained from the surface The paraffin that collects in the tray should not be reused The tray should be emptied every day Leica EG1160 Paraffin Embedding Center 13 8 Components and features Work area 20 The temperature of the work area is adjustable from 45 C to 70 C Itincludes the embedding area forceps holder 11 recessed area for cassettes 10 and space to remove the lids The forceps holder 11 is separately heated The heated recessed area Is designed for approximately eight cassettes By closing the drain hole with the stopper provided it can be utilised as a paraffin tray Several grooves and drain holes in the area where the lids are removed provide that molten paraffin drains rapidly Main switch 21 The main switch a green toggle switch is located on the back of the in strument It is not necessary however to turn the instrument off with the main switch after daily operation In daily routine the tissue embedding center should be switched on and off with the ON OFF button on the control panel Note If the Leica EG 1160 s automatic turn on feature is used I e if it is pro grammed to turn on automatically to be ready to operate when the user arrives the main switch must be in
21. is still approximately 1 litre left However the paraffin reservoir should be re filled immediately at that stage The set temperature for the paraffin reservoir should be set to a value between the melting point and the maximum value indicated by the manufacturer of the paraffin that Is used Once all functional units have reached the preselected tempera tures and the wax in the paraffin res ervoir is completely molten tissue embedding can begin Note Overheating of the molten wax must be avoided as important components can be damaged resulting in damage to the sample 24 1 The embedding cassettes contain ing the dehydrated and infiltrated tis sue sample are transferred to the cassette bath approx 100 cas settes which is filled with molten wax to prevent the solidification of the wax The cassette bath temperature Is optimally maintained constant when itis filled with molten paraffin lf the cassette bath Is not filled with wax it should not be filled with cas settes up to the top edge as a film could form on the cassettes at the top reducing the quality of the em bedding By use of the optional vacuum at tachment for the cassette bath the tissue can be infiltrated under vacuum The vacuum is generated by a vacuum pump that Is connected to the connecting branch on top of the vacuum lid The seal of the lid must be free of paraffin to be seated flat on the cassette bath to allow for the vacuum
22. mer 230V 50Hz acc to IEC 127 2 Capacities Cassette bath Work surface Power consumption 800 VA Paraffin reservoir 3 Cold plate Cassette bath approx 100 cass Workdays Operating Heated Daily work times beginning end temperature range 18 35 recessed area 8 cassettes Weekday Cold plate approx 60 blocks Time Cooling system Refrigerant 140 g 5g R134A Operation either manual or via the Compressor oil 150 ccm foot pedal EMKARATE RL15S Temperature control Functional unit Temperature Heated Separate Warm up time range separately temperature prior to operation control Paraffin reservoir 45 70 C 1 C increments Mold warmer 35 70 C 1h 1 C increments Cassette bath 45 70 C 1 5h 1 C increments Work area 45 70 C 1h 1 C increments Forceps holder left 70 C not basic instrument Paraffin dispenser 45 70 C pump at an ambient temperature of 22 C and at an air humidity of 60 All heated components include overheat protection 6 Instruction manual V4 0 4 98 5 General description Features 1 Paraffin flow rate is pump con trolled 2 Paraffin flow rate adjustable in 10 increments 3 Paraffin quantity level indicated in the display 4 Separate paraffin collecting tray 5 Removable cassette bath 6 Forceps holder on the right ba sic instrument or on the right and left not basic instrument 7 Paraffin dispenser with inte grated illum
23. n after several weeks When the instrumenthad been switched off with the main switch and was out of use for several weeks please pursue the same procedure as described above 2 After initialisation the language selection reads in the display The SPEAK ENGLISH ICH SPRECHE DEUTSCH desired language E S can be selected within 10 15 seconds pushing the ARROW UP ARROW DOWN buttons Once the button is relased the selected language Is stored automatically i 3 Approximately 10 seconds after releasing the ARROW button the in strument automatically switches over to the standby In the standby condition the display is not illuminated In the lower line of the display reads actual day and actual time as well as the current paraffin quantity level The upper line remains empty IP lw P P P TU 13 09 IT Day Time Quantity 4 The Leica EG 1160 is put into operation by pushing the ON OFF but OFF ton This button must be held down for approx 10 seconds This fea ture protects against unintended setting into operation The instru ment will then be ready for operation in the ACT mode PAR TANK 58 C TU 13 11 BENE Default reading Push one of the ARROW buttons to display the FLOW RATE reading In order to obtain a smooth bubble free paraffin flow the flow rate should first be set to 100 pushing one of the ARROW buttons Push eile the dispenser handle backward or press the foot pedal until the pa
24. n spot 16 Dispenser handle 9 L Cassette bath 15 Paraffin collecting tray 18 Heated area for Work area 20 cassettes 10 Forceps holder 11 10 Instruction manual V4 1 05 2001 1 Leica EG 1160 Overview Fuses Main switch 21 Socket for foot pedal gt Spacers Condenser fins gt a of Fs Power cord Areation knob 28 Vacuum attachment 25 Magnifier 24 Conection branch 27 Lid 26 The Leica EG 1160 is a compact benchtop unit It is available in two different configurations basic instrument and model with an additional forceps holders on the left Both models are shipped in a wooden crate Please check if the following accessories are included in the shipment and if they are in perfect condition NOTE If equipment or accessories are found to be damaged please keep the original packing material and contact your local sales office immediately Piecs Accessory Component Catalog Number 1 COMI E E 0386 19544 BUDE EMT TT TU 0356 08793 2 0386 24590 1 SEUOMSIO MP LO DES sessuali misit vnu ra rtt ud eut RE 0386 24782 Replacement bulb nnne nnne nnn nnns 0187 21220 Optional accessories S 0386 21462 Vacuumattachnment 0356 21980 FIDEFODUCALIIGMEGUIGG 0386 31352 1 Coldlight source Leica CLS 100 100 V 50 60 2
25. n the operator starts working see chapter 4 Technical data SET TEMPERATURES The MENU hutton is used to scroll through the individual items of the menu Once the last item of the menu WORKDAYS ARE is reached the first item SET TEMPERATURES PAR TANK will be indicated again after pressing the MENU button SET TEMPERATURES COLD PLAT XX C SET TEMPERATURES CASS BATH XX C SET TEMPERATURES MOLD TRAY XX C SET TEMPERATURES WORK SURF XX C TODAY 15 MO THE TIME 18 10 00 HRS START WORK AT 10 00 HRS STOP WORK 16 00 HRS WORKDAYS MO YES SET TEMPERATURES PAR TANK XX C etc 20 Instruction manual V 4 1 05 2001 10 Operation SET Mode Programming The ARROW buttons are used to preselect the temperature time and days The speed of the display increases 1 2 step mechanism the longer the button is held down 6 1 TEMPERATURE SETTING The temperature is preselected with the ARROW buttons Once the minimum or maximum value is reached the indication will stop The value displayed when releasing the ARROW button will be stored automatically and heating and cooling will be activated accordingly The individual temperature settings can be activated or deactivated with the ON OFF button ON The previously entered temperature reads in the display If the cur rent value is not changed the microprocessor will adjust the tem perature to the dis
26. om 35 to 70 C The hinged lid can be locked in position while open NN Cassette bath 15 The cassette bath temperature is adjustable from 45 to 70 C The cas sette bath can hold more than 100 cassettes It can be easily removed An integrated lid protects against thermal loss and contamination 0 igl Refrigeration spot 16 The refrigeration spot is integrated in the cold plate thus ensuring con sistent low temperatures Positioned directly in front of the embedding area it ensures convenient filling of the molds in optimal ergonomic con ditions The mold containing the sample is filled approximately one third and placed on the refrigeration spot where the paraffin starts to solidify rapidly In the semiliquid paraffin the sample can be oriented as required After orientation the mold is filled up with paraffin Note Care should be taken thatthe paraffin does not become too solid during orientation as this could cause an inhomogeneous block which would make sectioning more difficult Cold plate 17 The cold plate is turned on and off via the menu The temperature is 5 C The rate of cooling at 5 C ensures optimal consistency of the blocks and minimises the risk of brittleness as a result of too rapid cooling and a high level of productivity The cold plate provides ample space for ap proximately 60 paraffin blocks Controlled cooling ensures that the preselected temperature is accurately maintained Before start
27. played value OFF The temperature control function can be switched off if no specific temperature is required The previously entered temperature remains in the memory but will not be activated The display reads In this case the default reading is as follows 6 2 ACTUAL TIME DAY Actual time and day are set with the ARROW buttons An endless search mechanism provides that the search run restarts from the beginning automatically after attaining 23 59 or SUN The time day indicated on releasing the ARROW buttons will be stored automatically 6 3 BEGINNING AND END OF WORK The time when you start and stop work is selected with the ARROW buttons The endless search mechanism provides thatthe search run automatically restarts from the beginning after attaining 23 59 Thus the instrument will be operational on all days of the week defined as workdays see 6 4 at the previously selected time The time indicated on releasing the ARROW buttons will be stored automatically Leica EG1160 Paraffin Embedding Center gt SET TEMPERATURES PAR TANK ON OFF 58 C SET TEMPERATURES 58 C SET TEMPERATURES PAR TANK PAR TANK FR 11 10 TODAY 15 MO THE TIME 15 12 30 START WORK 10 00 STOP WORK AT 16 00 Tl z HRS HRS HRS 21 10 Operation SET Mode Programming 6 4 WORKDAYS The workdays are selected with the ARROW buttons Releasing the but Sanne ton th
28. r affin flow is smooth and free of bubbles 0 m The Leica EG 1160 has two modes of operation the actual operating mode ACT and the programming mode SET To change from one mode to the other push the ACT SET button gt Leica EG1160 Paraffin Embedding Center 17 10 Operation ACT Mode Operation D Normal operation of the Leica EG 1160 takes place in the ACT mode In this mode all display readings are actual values Starting from the default reading PAR TANK XX C each step of the 5 step menu of the temperature controllable functional units can be dis played to review the actual temperature by scrolling the menu with the MENU button TU 131 The status line always is the same aso COLD PLAT XX C TU 13 11 EEEE CASS BATH XX C TU 13 12 EEEE MOLD TRAY XX C TU 13 12 BEEE All display readings both in the ACT and SET programming mode auto ETE matically return to the ACT default reading after approximately 10 sec TU 1312 onds PAR TANK XX C TU 13 15 EBEN 5 1 The ILLUMINATION of the work area is switched ON OFF with the LAMP button 5 2 The PARAFFIN FLOW RATE is individually adjustable and can be 5 3 Adjustment of the FLOW QUANTITY 18 preselected in the ACT mode with the ARROW buttons in 1096 increments FLOW RATE 10096 TU 13 18 EEEE The flow quantity for the lowest flow rate 10 can be adjusted a
29. required tem peratures This applies particularly to the heater of the paraffin reser voir as It can take several hours un til the solid wax Is entirely molten The warmup times of the individual components are listed in chapter 4 Technical data 25 12 Cleaning maintenance service Regular cleaning and maintenance will keep your Leica EG 1160 In good operating condition for many years 1 General instructions Paraffin Paraffin is flammable and therefore must be handled with care Avoid spillage of liquid paraffin All Leica EG 1160 components that come into contact with paraffin and the interior of the instrument are carefully sealed to prevent wax from entering Nevertheless if paraffin is spilled it should always be removed carefully paraffin reservoir and cassette bath if required should be filled with care Avoid overfilling wax in the cassette bath and heated recessed area must be ex changed every day to avoid contamination Solid wax particles on the surface of the work area must not be removed with sharp tools as this could damage the finish A soft plastic spatula Is ideal for wax removal Alternatively solid paraffin can be lifted off easily by lightly warming it surfaces of the work area of the Leica EG 1160 are made of alu minium with polyester epoxy finish The control board is covered with a PE film The base of the housing is a polyester epoxy coated s
30. s re quired All other flow rate increments will then be recalculated on this basic adjustment and stored automatically FLOW RATE 10 Setthe flow rate to 10 by pushing the ARROW buttons in the ACT mode LLL On pushing the SET ACT button the display reads Decrease the flow rate to 1096 Operate the pump manually or with the foot pedal and push the ARROW i buttons to readjust the flow quantity for the 10 flow rate The new val ACT ADJUST ues will be stored automatically FLOW RATE 1096 Increase decrease the flow rate Instruction manual V4 1 05 2001 10 Operation ACT Mode Operation 5 4 The paraffin flow rate is selectable in 10 incre ments 10 96 20 96 30 40 96 50 60 96 70 96 80 96 90 95 100 90 Increase decrease 4 of the flow rate The display reading will change with the selected flow rate It always reads the percentage flow rate FLOW RATE 80 selected TU 1315 BEEN How to quit the display reading FLOW RATE FLOW RATE 80 10 sec TU 13 15 ENEE DI 1315 HEEE 1 Approximately 10 seconds after having selected the flow rate the display will automatically return to the ACT default reading FLOW RATE 80 96 XX C 2 Pushing the MENU button will also lead to the ACT We Ta EHE DI 1315 BEEN default reading FLOW RATE 80 TEMPERATURWAHL 3 Pushing the SET ACT button will lead to the pro IE HM meee ACT Di 13 15 HENE gramm
31. teel plate All seams are sealed with a specific fungicidal silicone All materials are easy to clean with common laboratory detergents which are appropriate for paraffin removal Do not allow organic solvents to reactfor a longer period Apply varnish protection occasionally Caution Xylene must not be used for cleaning Although xylene is an ideal wax sol vent its flash point ranges between 27 and 32 C Xylene vapours are heavier than air and can catch fire even if the source of heatis ata larger distance Therefore it is not appropriate for cleaning hot or potentially hot surfaces Risk of fire 26 Instruction manual V4 1 05 2001 12 Cleaning maintenance service 2 Cleaning of paraffin reservoir filter and dispenser outlet The grid separating solid from liquid wax and the filter can be removed from the paraffin reservoir for cleaning The reservoir is cleaned inside with a paper tissue Care should be taken that the reservoir is contaminated Any dirt inside should be removed prior to removing the grid and the filter 3 Cleaning the forceps holder The forceps holder in particular is frequently a source of contamination and is susceptible to dirt Therefore it should be cleaned thoroughly Caution The forceps holder is heated separately and thus very hot approx 70 C or 80 C during operation 4 The paraffin drained to the paraffin collection tray should be emptied regularly to ensure that excess p
32. the ON condition Note After a power failure the Leica EG 1160 will not switch on automatically At that stage it is in the standby condition just like after turning it on with the main switch i e you have to set it going pushing the ON OFF switch Cooling Whenever the instrument was disconnected from mains for example after a power failure or when it had been turned off with the main switch it will take 10 minutes until the condenser of the cooling system is ready to oper ate Cover cap for illumination 23 The cover cap for the light bulb is inserted at the front of the paraffin dis penser For light bulb replacement the cap is pulled towards the operator 14 21 10 11 TL 1494 Instruction manual V4 1 05 2001 8 Components and features Instrument with additional forceps holder on the left Forceps holder left 11a The instrument configuration with an addional forcesp holder on the left covers the needs of left handers in particular This forceps holder also is separately heated Optional accessories Magnifier 24 When correctly mounted the magnifier can be swung horizontally and vertically When correctly adjusted both the paraffin dispenser outlet and the refrigeration spot are in focus thus avoiding frequent readjust ment to orient small and delicate biopsies Vacuum attachment 25 The optional vacuum attachment can be used instead of the
33. the paraffin outlet nozzle The flow is adjustable in 10 incre ments Instruction manual V4 0 4 98 11 Tissue embedding 5 Open the cassette and transfer the sample to the mold using warm for Ceps 6 he mold is placed on the refrig eration spot for a moment The tis sue is oriented with forceps as re quired Within a few seconds the clear consistency of the wax will change Small tissue specimens can be ented by slowly pushing the mold from the embedding spot to the re frigeration spot As the wax solidifies very slowly in the transition area the tissue can be removed from the for ceps without any risk thatthe sample sticks to the tip of forceps If the wax becomes solid too rapidly the for ceps will stick to the tissue Note Please avoid a film to form on the surface of the paraffin This would create two phases in the fin ished block which could cause fis sures inside as a result of which block could break during sectioning 7 After orientation of the specimen the half filled mold is retrans ferred to the embedding spot After posi tioning a cassette base or an embed ding ring the mold is filled up with paraffin When notusing cassettes or embed ding rings the mold should be filled up to the top edge Note Do not overfill the mold to avoid contaminating the cassette base or embedding ring outside as this could result in the insufficient clamping in the specimen holder of th
34. to build up To improve the tightness the lid should briefly be pushed on to the cassette bath when the vacuum pump is turned on After infiltration the lid cannot be opened immediately The aeration knob has first to be opened slowly to allow for pressure equalisation inside 2 The embedding molds are placed in the mold warmer Note Overheating of the molds must be avoided as this could cause prob lems when removing the block from the mold The molds should be clean and dry First the molds should be treated with xylene then with soap solution and finally rinsed with distilled wa ter and dried 3 Take three to four cassettes out of the cassette bath and depending on the work method either place them on the work area to allow liquid par affin to drop and immediately remove them from the mold or transfer them to the heated recessed area filled with molten wax for intermediate storage to prevent immediate solidi fication of the infiltrated specimen This applies in particular to small bi opsies If the heated recessed area is not filled with wax it provides ad ditional space on the work area 4 Select an appropriate embedding mold place it under the dispenser outlet nozzle and fill it with paraffin as required a using the foot pedal b manually by pushing the paraf fin dispenser handle c when filling larger molds the dis penser handle can be retracted thus providing enough clearance under
35. uential damage caused by or related to compliance with statements or other infor mation in this manual statements drawings illustrations and other informa tion as regards contents or technical details of the present manual are notto be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between our selves and our customers Leica reserves the right to change technical specifi cations as well as manufacturing processes without prior notice Only in this way is it possible to continu ously improve the technology and manufacturing tech niques used in our products This documentis protected under copyright laws Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods including any electronic systems and media requires express prior permis sion in writing by Leica Microsystems Nussloch GmbH For the instrument serial number and year of manu facture please refer to the name plate at the back of the instrument Leica Microsystems Nussloch GmbH So SSS o Te _ Spe ques Table of contents Important iet n cn E tpa n dtes 3 IE of contents c 4 installation o
36. uide eds i b bd Cu 0386 19544 FCU NNNM ZZ 111 QD 1I mc LI 0386 21462 nmeplacementbHlD 0187 21220 0386 21980 StOBBePSIDaCKOLdU o o tos 0386 24782 Fiber optical light guide without coldlight source _ 0386 31352 Coldlight source Leica CLS 100 0502 30213 50 0502 30214 0502 30215 50 6 ETZ 0502 30216 Embedding cassettes without 250 pcs white 0394 12312 ELE A AR EE 0394 08972 ye IOW eina A 0394 08974 redana TEE 0394 08976 OCI R 0394 08978 0394 08980 Lids for cassettes 250 pcs UI LEER 0394 12315 0394 08982 Redes bod nup Peri Nd dnt 0394 08986 Eee UU CENE 0394 08988 0394 08990 Biopsy cassettes 250 pcs AV UICC P 0394 08966 Lids for biopsy cassettes 250 pcs dos ted 0394 08970 Deep lids for cassettes for samples up to 12 mm high 250 pcs white see 0394 12767 Embedding rings 1000 pcs WAI E ii Ni 0386 12392 Embedding molds for samples up to 0386 12302 5 153615
37. y qualified personnel only P Operating Instruction Manual avoid any operating mistakes from the beginning it is necessary to read the operating instructions to familiarize oneself with the technical details Paraffin is flammable and should be handled with care Spillage should be avoided Paraffin on the surfaces must not be removed with sharp tools as it would ruin the coating It should be avoided to allow xylene to react on all surfaces Caution Xylene is a flammable organic solvent Its flash point is between 27 and 32 C Xylene vapours are heavier than air and can easily catch fire on hot surfaces or sparks even over a greater distance Illumination Before replacing the lamp the tissue embedding center must be turned off with the main switch To clean the condenser fins switch the instrument off with the main switch and disconnect it from mains To replace the fuses the power cord must be disconnected from the power supply Only fuses that are accessible from outside can be re placed If you need further information please contact your local Leica sales office or dealer When operating the paraffin reservoir mold warmer cassette bath work area and forceps holder are all hot Caution Risk of burning Note Combustible and flammable substances must not be stored near the instrument 8 Instruction manual V4 1 05 2001 6 Safety precautions When using the optional
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