AZURA Pump P 2.1L user manual
Contents
1. Double click on LAN Connection Click on the button Properties Select Internet Protocol version 4 TCP IPv4 Click on the button Properties NO Un BR W NN Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP address automatically b Obtain DNS server address automatically 7 Click on the button OK Connecting the Cables A router has several LAN ports and one WAN port that can be used to integrate the LAN into a wide area network WAN e g a company network or the Internet On the other hand the LAN ports serve to set up a network from devices and a computer To avoid interference we recommend to operate the HPLC system separate from the company network You will find patch cables for each device and the router in the accessories kit To connect the router to a WAN an additional 33 Legend Modules Router LAN port WAN port Workstation Prerequisite Procedure Procedure Result Prerequisite Procedure Initial Startup patch cable is required which is not supplied within the scope of delivery E al Ral A WAN Ss Fig 34 Cabling system LAN Computer is on There is a patch cable for each device and the computer 1 Use the patch cable to connect the router and the computer Repeat this step to connect all devices 2 Use the power supply to connect the router to the main2. Prerequisite Procedure Tools Maintenance and Care Screw Fittings on the Pump Head Fig 37 Screw fittings on the pump head Leaks in the Capillary Screw Fittings CAUTION Damage to the device possible If leaks occur after maintenance and assembly replace the capillary connections with new ones Replacing the Pump Head Depending on the requirements of the user different pump heads are used The chapter accessories and spare parts contains a pump head overview The pump head has been purged Hoses are disconnected Loosen the capillary fittings Loosen the Allen screws on the pump head Remove the pump head Allen wrench size 3 Open end wrench sizes 10 17 Dismounting the Pump Head WARNING Aggressive or toxic solvent residue can irritate the skin Wear protective gloves Flush the pump head before exchanging it CAUTION Damage to the pump pistons due to jamming of the pump head Loosen diagonally opposite fastening screws evenly by one turn 43 Maintenance and Care Dismounting the Process Figure pump head To remove the capil lary loosen the capil lary screw fittings at the pump head outlet and pressure trans ducer inlet Disconnect the hoses of the piston backflushing from the flush pump and the pump head Remove the eluent lines from the eluent Fig 38 Removing the pump inlets head Unscrew the Allen screws Hold the pump head by han
3. In case you notice any damage contact the technical support and the forwarder company 14 Unpacking and Setup 2 Setup the delivery so you are able to read the label Using the utility knife cut the adhesive tape Open the delivery 3 Remove the foam insert Take out the accessories kit and the manual 4 Open the accessories kit and take out all accessories Check the scope of delivery In case any parts are missing contact the technical support 5 Grip the device at its side panels and lift it out of the packag ing Do not hold onto front cover or leak tray 6 Remove the foam inserts from the device 7 Check for damages caused during transportation In case you notice any damage contact the technical support 8 Set up the device in its location 9 Remove the protective foil Ports on the Rear Side All connectors are located on the rear side of the detector Legend Serial number Pin header LAN port wi Interface for the corsa Technical Support KNAUER Mains power con wimini nection and power wi switch Connector Control Unit Fig 2 P 2 1L rear view o Fig 3 Connector Control Unit 15 Unpacking and Setup There are two ways to operate the pump Control with pin header Control within a local network through a router Controlling with the Pin Header CAUTION Short circuit hazard Turn off the device before connecting it to the pin header Pull the
4. 24V DC Analog GND Reference point of the voltage at the signal inputs Analog out Voltage range 0 5 V scalable Analog in Voltage range 0 10 V 10 V according to maximum flow rate ay 17 Unpacking and Setup Connection Function Start GND Reference point of the voltage at the signal inputs Start OUT TTL output Levels passive 5 V active 0 V Start IN TTL input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an external device the device Starts If controlled with software an elec tronic trigger is send through the LAN Error OUT TTL output Levels passive 5 V active 0 V Error IN TTL input Low active Secure switching threshold at least 10 mA After receiving a signal short circuit to ground from an external device an error message appears and the device stops Error GND Reference point of the voltage at the signal inputs 24V Event controlled switching of 24 V against GND Protection 24 V 200 mA 5V Provides a voltage of 5 V with respect to GND This makes it possible to supply a con sumer that is switched by an EVENT Protection 5 V 50 mA Reference point of the voltage at the signal Analog Control Analog ports serve for exchanging analog control signals Refer ence point for the signals is the connector GND OUT Device sends signal IN Device receives signal 18 Initial Startup Functional princi
5. Center LED does not light Device is switched Switch on the off device flashes green Device not ready for Wait until the measuring device is ready green Device is switched on Right LED green Device active or ready for measuring Device in standby Press the switch to end the standby 40 Installation Qualification IQ Installation Qualification IQ Installation report Certification on the functionality of the device During installa tion of the device an installation report IQ document Is cre ated upon request in coordination with the technical support of the manufacturer This installation report needs to be completed in full and signed by both parties It serves as proof of the properly executed installation and the functionality of the device Operation Qualification OQ Extensive functionality test Note Extensive test of the detector s functionality A successfully exe cuted OQ ensures that the detector functions properly Test Intervals Run the device test at the following time intervals Average useful life 1 to 5 days week Every 6 months More than 5 days week or 24 hours day Every 3 months Operation with buffer solutions or other salt solutions Execution The execution is done either by the manufacturer s technical ser vice or by a technical service authorized by the manufacturer The OQ documentation required for executing the OQ is with costs once and can be
6. glasses when working on the device Never use the system in potentially explosive atmospheres with out appropriate protective equipment For further information contact the Technical Support of KNAUER At any time take the device completely out of operation by either switching off the power switch or by pulling the power plug The device may only be opened by the Technical Support of KNAUER Definition of Personal and Material Dam ages Possible dangers related to the device are distinguished in per sonal and material damages in this user manual delia Personal damages and material damages DANGER Lethal or very serious injuries can occur CAUTION Moderate injuries can occur and device defect can occur 11 Symbols and Signs Symbols and Signs Marks Warning signs Mandatory signs Device fulfills the requirements of the Con formit Europ enne which is confirmed by the Declaration of Conformity Damage to the leak tray or front cover possible while carrying setting up and installing a device Grip the device at its sides near the middle when lifting or mov ing High voltage hazard Electrostatic discharge hazard Wear protective bracelet against electro static discharge and ground Pull the power plug Observe torque of 5 Nm maximum Wear protective gloves 12 Scope of Delivery Scope of Delivery Note Only use original parts and accessories made by the manufac turer or a
7. in case the system message repeats itself rr l Instrument in stand Change the entry in the Setup alone mode menu Change the entry in the chroma tography software Instrument remote This entry is not executable Quit soft controlled ware Invalid index in time Change the entry in the program line table Invalid line number Change the entry in the program line Invalid time in time Correct the time entry table L Leak sensor not pres Switch the device off and then on ent If the leak sensor is still not present contact the Technical Support of the manufacturer Leak was detected Switch off the device Remove the leak and start the device afterwards Line in time table is Edit the program line empty Link is loaded First unload the link then change the link or delete it Link is running Wait until the link has been com pleted then change the link or delete it M Max flow limit Confirm pump continues running reached 54 Troubleshooting Maximum pressure Reduce the pressure or adjust the System stopped upper pressure limit Restart the system Minimum pressure Increase the pressure or adjust the System stopped lower pressure limit Restart the system Motor failure max Switch the device off and on Inform current the technical support of the manufac turer in case the system message repeats itself N No gradient is avail Change the setup settings or change ab
8. is controlled with Control Unit or chromatography soft ware With decreasing polarity of the eluent the sensitivity of the leak sensor must increase Non polar eluents Setting high for e g hexane heptane Polar eluents Setting low for e g water Deactivating the Leak Sensor In case the leak sensor detects leaks without reason and con stantly sends error messages which make working or repair ing the device impossible n case you want to continue working even though leaks were detected Device is controlled with Control Unit or chromatography soft ware Eliminating the Leak 1 Eliminate the leak 2 Dry the surface of the collection point 26 Initial Startup 3 Confirm the error message Result The device is ready for operation Holding Bracket You can mount a holding bracket onto the side panel and attach valves columns or flow cells to it Abb 23 Holding bracket with valve Following accessories can be mounted onto the side panel Accessories Comment Valves KNAUER valve Mounting directly at the holding bracket VICI Valco valve Mounting directly at the holding bracket VICI Valco valve drive Mounting at the holding bracket by using the adapter plate Flow cells KNAUER flow cells Mounting at the holding bracket by using the adapter plate Columns Axially compressible col Mounting at the holding bracket umn Vertex Plus AX by using the adapter 20 mm and 30 mm Sample loops Preparative samp
9. is mounted 32 Note Process Prerequisite Procedure Note Initial Startup Control the Devices with a Computer in a Local Area Network LAN This chapter describes how to set up a local area network LAN and how a network administrator can integrate this LAN into your company network The description applies to the operating system Windows and all conventional routers To set up a LAN we recommend to use a router The following steps are necessary 1 On the computer go to the control panel and check the LAN properties 2 Hook up the router to the devices and the computer On the computer configure the router to set up the network KW Install the chromatography software from the data storage device 5 Switch on the device and run the chromatography software Configuring the LAN Settings The LAN uses only one server which is normally the router from that the devices automatically receive their IP address In Windows power saving hibernation standby and screen saver must be deactived n case you use an USB to COM box the option Allow the computer to turn off ths device to save power in the device manager must be deactivated for all USB hosts Only for Windows 7 For the network adapter the option Allow the computer to turn off ths device to save power in the devicemanager must be deactivated 1 In Windows 7 choose Start Control Panel gt Network and Sharing Center
10. knauer net ll KNALIER KNAUER 2012 V6840A 0 01 10 12 Koe
11. power plug Plug Connector Assignments Connection Function TTL compatible output Levels passive 0 V active 5 V Pulse 5 V for at least 1000 ms TTL compatible output Levels passive O V active 5 V Pulse 5Vfor at least 1000 ms TTL output Levels passive O V active 5 V Pulse 5Vfor at least 1000 ms Reference point of the voltage at the signal inputs TTL compatible output Levels passive O V active 5 V Pulse 5V for at least 1000 ms 16 Unpacking and Setup Connection Function STTL TTL compatible output Levels passive O V active 5 V Pulse 5V for at least 1000 ms 60C TTL output Levels passive O V active 5 V Pulse 5Vfor at least 1000 ms Reference point of the voltage at the signal inputs Relay contact The contact is on a floating basis Its setting depends on the settings in the Control Unit or software Steady rate signal passive open relay contact active closed relay contact e Pulse Closed relay contact for at least TL 1000 ms Permissible load of the relay contact 1 A 24V DC Relay contact The contact is on a floating basis Its setting depends on the settings in the Control Unit or software Steady rate signal passive open relay contact active closed relay contact e Pulse Closed relay contact for at least fl 1000 ms Permissible load of the relay contact 1 A
12. sys tem message repeats itself First stop the ink and then edit the data on the device display or with the chromatography software First pause link then delete program First pause link then edit data using chromatography software Cannot initialize LAN Cannot operate with an empty link Cannot purge dur ing the run Cannot read data from FRAM Cannot read RTC Cannot start time table Check cables and connections in local area network Create a link End method and start purging Switch the device off and on Inform the technical support of the manufac turer in case the system message repeats itself Switch the device off and on Inform the technical support of the manufac turer in case the system message repeats itself Edit the data on the device display or in the chromatography software 53 Troubleshooting Cannot use non Change the setup settings or change existing component the gradient in the program or in setup Cannot write data Switch the device off and on Inform on FRAM the technical support of the manufac turer in case the system message repeats itself Component set Change the setup settings or change tings not compatible the gradient in the program or in with gradient setup setup Error input activated Device error change device settings G GUI communication Switch the device off and on Inform failed the technical support of the manufac turer
13. tor Son Device will not Inspect the power cable to ensure that it turn on is plugged into the power supply When purging the Check if the ventingion screw on the pump switches off pressure transducer is turned up Pump does not Purge the pump head to remove the transport solvent air bubbles Clean the check valves Exchange the check valves If the pump head seals are defective solvent enters the piston backflush ing inform the technical support of the manufacturer Exchange the pump head Pressure and flow Purge the pump head to remove the rate variations air bubbles Always tighten the inlet screw 1 and outlet screw 1 on the pump head with a torque wrench and 15 Nm Clean the check valves Exchange the check valves Exchange the pump head Inform the Technical Support of the manufacturer 51 Troubleshooting Pump head leaks Inspect the inlet and outlet screw fit tings of the pump head If the seals are defective eluent enters the piston backflushing inform the Technical support of the manufacturer Exchange the pump head Flow rate is not Check the following options correct Check the data for the solvent com pressibility Clean the check valves Exchange the check valves System Messages If other system messages are displayed besides those listed below please turn the device off and then on Inform the Tech nical Support of the manufacturer in case the system message repeat
14. 1 Types Note Legend Valve block Screw with seal ring Note Prerequisite Tools Mounting the valve block Initial Startup Changing the Setup to LPG By assembling a valve block the setup of the system is changed to low pressure gradient LPG The valve block is mounted to the front of the pump There are 2 types of valve blocks Ternary valve block for flow rates from 10 250 ml Binary valve block for flow rates from 10 800 ml min The manufacturer recommends to employ the binary LPG valve block for flow rates in the range of 100 800 ml min Fig 11 Ternary valve block You can not use the front cover after having mounted the valve block Mounting the Valve Block Always place seals in pairs on both fastening screws Pump has been switched off Power plug has been pulled Open end wrench size 17 Torque wrench Process 1 Unscrew the inlet from the pump head Fig 12 Inlet on the pump head 22 Initial Startup 2 Place the seal rings on the screws and valve block Using the torque wrench tighten the screws of the valve block with a 7 5 Nm torque D Fig 13 Valve block Insert the plug into the female connector Fig 14 Socket for the LPG valve block Connecting the valve block 1 Push the Teflon hose OD 0 through the fastening screw and the cut ting ring E Insert the hose end as far as possible into t
15. 1010 1 2010 Corrigendum 2011 Safety require ments for electrical equipment for measurement control and laboratory use Low voltage directive 2006 95 EC EN 61000 3 2 2005 A1 2008 A2 2009 Electromagnetic compatibility EMC Part 3 2 EMC standard 2004 108 EC IEC 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements EN 61326 1 Corrigendum 2 2011 Directives for an environmentally sound use of electrical and electronic equipment ROHS directives 2002 95 EC 2003 and 2011 65 EU on the restriction of the use of certain hazardous substances in electrical and electronic equipment WEEE directive 2002 96 EC 2003 on waste electrical and electronic equipmenta The product was tested with a typical configuration Berlin 10 31 2012 Ale Dr Alexander B nz Managing Director The mark of conformity has been applied to the rear panel of the device CE Table Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig of Fi 5 6 e 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 59 Table of Figures gures Pump PZ WE FE
16. 30 Initial Startup Mounting a VICI Valco valve drive Prerequisite Holding bracket is attached to the side panel Tools Allen screwdriver for 9 64 Process Procedure 1 Screw the adapter plate on the rear side of the holding bracket Slide the valve drive and the adapter plate from the rear to the front through the hole of the hold ing bracket as far as possible Fasten the valve with four screws to the front side o Abb 30 Back side of valve at the holding bracket Abb 31 Front side of valve at the holding bracket Result Valve drive is mounted 31 Initial Startup Mounting a Flow Cell Prerequisite Holding bracket is attached to the side panel Process C Procedure 1 Position the adapter plate on the rear side of the holding bracket 2 Position the flow cell to the front of the holding bracket 3 Fasten the flow cell with both knurled head screws in position Abb 32 Flow cell with adapter plate at the holding bracket Result Flow cell is mounted Mounting the Axially Compressible Column Vertex Plus AX 20 mm 30 mm Prerequisite Holding bracket is attached to the side panel Tools Allen screwdriver size 4 Process Procedure 1 Place the column inside the holder 2 Position the holder onto the bore holes of the holding bracket 3 Tighten the screws and washers Abb 33 Column holder with hold ing bracket Result Axially compressible column
17. ANNs A oe Ae ns cele 29 FIONL VICW opu editarii ei a eed 4 Date tebe ee eh toe eas 29 Back side of valve at the holding bracket 30 Front side of valve at the holding bracket 30 Flow cell with adapter plate at the holding bracket 31 Column holder with holding bracket 31 Gabiing systent LAN cess ars o adi 33 POW S WILD Siew es sh eee Sie 36 LEDs and switch on the front of the device 38 Screw fittings on the pump head o o oo ooooocoooo ooo 42 Removing the pump head 0 ee ee eee eee ee 43 SEWN asa oa Bee eee ee 43 PUNO GAG pegis ee that aca ve eg hse oe aus Woe Gee te Sees 44 Removing the check valve 2 0 eee ee ee ee 44 BCheck ValVeall valve acc 6eetee tee Soe tos A 64 So eSeR se 45 60 Index Index A Accessories 12 Additives 8 Analog control 17 AVV marking 57 C Caring 45 CE sign see Declaration of Conformity 58 Check valves 44 Clean 45 Install 45 Remove 44 Cleaning 45 Connections 18 Contact 41 Control Control Unit 38 Chromatography Software 38 Control lever 29 30 D Declaration of Conformity 58 Decontamination 9 Device Rear view 14 Disposal 57 E Eluent Flammability 9 Self ignition point 10 Toxicity 10 Eluents 8 E Flushing the pump 36 LPG System 36 G GROUND see spring strip 16 Installation Qualification 40 Intended use 6 IQ 40 L LAN Port 34 Rou
18. Ih KNALIER feura Pump P 2 1L User manual V6840A 3 Table of Contents Table of Contents Note For your own safety read the manual and always observe the warnings and safety information on the device and in the manual mended Use in a eben odiado asa 6 DEVICE OVEIVIEW atinada dit 6 O AAN 6 PUMO A ohare ain ea eos Fe Re ele ee ene eee a eee 7 El at re ada is BS ig Gs toes Ok Mensa eset en Oe 8 Safely TO USCIS alii 9 Definition of Personal and Material Damages oooooooo mo 10 SymboIs and SIGNS 0d edad iria a Rises 11 Scope Of Deliver a 12 Unpacking and Setup csm nds andas ic 13 Contacting the Technical Support 0 0 0 cee ee ee ooo 13 Location REQUIFEMECNIS se so ad acetal see pa wees Gees awa 13 UNPACKING es sess Saha carats alae ae dst denn el npise hia eh oar ie ahs Gee aaa te at eles oe hg es 13 POMS Om NG Rear Ide ctas eee ten ee he eh ea heeded eee kde pis 14 Controlling with the Pin Header 0 ce eee eee eee 15 Plug Connector Assignments victoria es 15 Analog Contiolasao str 17 Initial tartera ia ee ee eae es 18 Connecting the Piston Backflushing 2 cee eee ee ee ee eee 18 PIUCNU IMIG sostenida dos ende 19 Connecting the Eluent Inlet to the Pump Head 19 Connection of the Eluent Line o o ooooooooooooooo momo oo o 20 Changing the Setup to LP Gases sas tein ennega Eee ea sala 21 Mounting the Valve BIOCK sies
19. LUS MING MG PUMP sanos ete te cen oak ey palo wit agian Goda Batok wed ed 36 Op ra Nasa ato a senate ro a ls a iio 38 Control with Chromatography Software o o oooooooooooooooo 38 Control With CONTOLUNIC soria laredo diran 38 Meaning OF tne LEDS lt a oi ota ese Sd a a eS 38 Installation Qualification IQ oo ooooooo o o 40 Operation Qualification OQ oooooooooomo mo m oooo 40 TestiMtervals seriadas a a R A 40 EXECUTOR augusta sa ad ata aa 40 Maintenance and Care ooooooooooooomoor ono oonoo 41 SWIECHING OM the PUMO 232050 islas e st wee em ed 41 Contact with the Technical Support o ooooooooocooooooomooo 41 Technical Support Hotline vernos ti as 41 Maintenance Contracks asii as E A USAS iaa 41 What maintenance tasks can users perform on the device 41 screw Fittings on the Pump Head usina lira sd 42 Leaks in the Capillary Screw Fittings oo o ooooooooooo ooo 42 Replacing tne Pump Heddes 4 esos ct cote gx pies ads 42 Dismounting the Pump Head ce eee ee ee eee 42 Attaching Capillaries to the Pump Head 0 e eee ee eee 43 Maintaining the Check Valves 0 0 cece ne eee eee 44 REMOVING the CHECK Valves susana a 44 Cleaning the Check Valve cuicos ew He SES ee Se eae 45 Installing the Check Valve 0 ee ee eens 45 Torques for Inlet and Outlet Fittings 0 0 cee eee eee 45 Cle
20. ONEVIENW na ets dnd ae Reker E 6 PZ META VICW sia caridad heehee Goes eee ede 14 Connector Control Unit 0 0 ee eee ene 14 Mets MDOL sisi dd RS gl eee ida 18 OULIELSYIMDO codi db thee eee Ov Lee ee dae ae 18 Eluent inlet for pump heads from 100 500 ml 19 Eluent for 1000 ml pump head 02 e eee eee 19 EIUGNUIGICU varant l asses oo Sri da sidad de dd 19 Connecting the eluent line to the pump head 20 Eluent line and 1000 ml pump head 4 20 Tematy VaVe Dock cc 21 Mecon the pumo Nead isaac e Eare Eey 21 Valve DICK cena y are O Bre a N REAR A 22 Socket for the LPG valve block 0 0 eee ee ee eee 22 Cutting ring with Teflon hose o o oooooooooo ooo 22 Mets orte ValVE DIOCK ra BN a Be ede e 22 Venting screw of the pressure transducer 23 View of the drainage system 0c eee ee eee ee eee 24 Funnel and capillary guide o o oo ooooo momo oo 24 FIOSCANCINOZZIG ad cada estoi sida ida 24 hose connected to GeVICE eiii aa a FEES 25 Leak tray With NOZZE ori ars de e a ee ee dio 25 Holding bracket with valve o o ooooooooo oo omo ene 26 HOIGS SA Sr eae AS UBA ee ee oe ee a 27 Screws NOIGING DIaACkel esse cvewes eee eee eee eee wee ee eae 27 FIOM VICW gt cares de ee ke ee eke Sa eh eee ek eee 28 Real VIEW Sua ps betes ee wus Seu O e oes 28 REAL VIEW aio BOS ahora ese Ace eds co
21. aning and Caring for the Device o o ooooooocooooomomooo 45 Technical Data ese orb tt aa DA aia 46 5 Table of Contents Accessories and Spare PartsS o oooooooooooo ooo ro o 48 ACCESSORIES espa ad nd 48 Device VallallONs xs NA NA A aes Rs 48 Avallable PUTO Heads e srren bodice vse eas bac eee AEREA 49 TFOUDIGSHOOUNG sede Gk caw denne rada ds 50 Possible Problems and Rectifications o ooooooooooooo omo 50 Systemi Messages pura SR 8 eRe eS 51 Legal Information 22 cc66as ecb pt eidiort et bee heeeeidamseeed 56 Warranty Conditions s 00 05 0 bon ches daa anes wae tae 56 VITA CCUG canada aig bn a AMR laa 56 Transporation Damages a cueca aldea ee ee E 56 Environmental Protection ooooooooo momo oo oooso 57 DISPOSAl arrancador arder 57 Declaration of Conformity o ooooooooooo morros 58 Tavie OL FIQUIES sui ai dl EAS 59 6 Intended Use Note Legend Status LEDs Pump head Pressure transducer Operating range Location Intended Use Only use the device for applications that fall within the range of the intended use Otherwise the protective and safety equip ment of the device could fail Device Overview P 2 1L is a self priming pump with automatic piston backflush ing that is used in preparative HPLC systems Fig 1 Pump P 2 1L Front view As part of a HPLC system the pump takes part in separating substance mixtures and in filt
22. case there are any questions on spare parts or accessories Accessories Control Unit AZDOO User manual german V6840 User manual english V6840A Accessories kit AZURA accessories kit FZAO2 Device Variations Pump P 2 1L without pump head APE20 Pump P 2 1L with 100 ml stainless steel APE20KA pump head Pump P 2 1L with 100 ml titanium pump APE20KB head Pump P 2 1L with 250 ml stainless steel APE20LA pump head Pump P 2 1L with 250 ml titanium pump APE20LC head Pump P 2 1L with 500 ml stainless steel APE20MA pump head Pump P 2 1L with 500 ml titanium pump APEZOMC head Pump P 2 1L with 1000 ml stainless steel APEZONA pump head Pump P 2 1L with 1000 ml titanium APE20NB pump head 49 Accessories and Spare Parts Available Pump Heads fame Order number Pump head 250 ml titanium A4021V2 Pump head 500 ml stainless steel A4038 1 Pump head 500 ml titanium A4038V2 Pump head 1000 mi stainless steel A4022 1 Pump head 1000 ml titanium A4022V2 50 Troubleshooting Troubleshooting First measures Check all cabling Check all screw fittings 1 2 3 Check whether air has gotten into the supply lines 4 Check device for leaks 5 Pay attention to system messages Further measures 1 Install the maintenance software service tool 2 Save device information and send to manufacturer Inform the Technical Support of the manufacturer Possible Problems and Rectifications
23. company authorized by the manufacturer Delivery Pump P 2 1L Power Cable User manual german and english Installation Qualification Document Accessories kit AZURA Pump 13 Unpacking and Setup Unpacking and Setup Phone Fax E mail Requirements Note Tools Procedure Contacting the Technical Support You have various options to contact the technical support 49 30 809727 0 49 30 8015010 support knauer net You can make your requests in English and German Location Requirements CAUTION Defect of the device due to overheating Set up the device so that it is protected against exposure to direct sunlight Make sure the room is well ventilated Allow 30 cm space at the rear of the device for air circulation The location for the device must meet the following require ments Weight 19 kg Dimensions 361 x 201 x 523 mm width x height x depth Power Supply 100 240 V DC Air humidity lt 90 non condensing Temperature 10 40 C 50 104 F The leak sensor may malfunction it the device is placed on an inclined surface Use a level to check that the device is in an hor izontal position Unpacking CAUTION Damages to the device caused by carrying it on protruding device parts Lift the device only at its side on the housing Store all packing materials Included packing list should be kept for repeat orders Utility knife 1 Check for damages caused during transportation
24. contaminated All materials or fluids used for decontamination must be collected separately and dis posed of properly Flammability Organic eluents are highly flammable Since capillaries can detach from their screw fittings and allow eluent to escape it is prohibited to have any open flames near the analytical sys tem Leaks Regularly check if any system components are leaking Leak sensor Observe display on the device on the control unit and in the chromatography software 10 Safety for Users Solvent tray Eluent lines Self ignition point Power supply Toxicity Where is use of the device prohibited Take the device out of operation Opening the device Explanations Risk of electrical shock or short circuit if liquids get into the device s interior Place all bottles in a solvent tray Install capillaries and hoses so as to liquids can not get into the interior in case of a leak If several devices are connected to one power strip always consider the maximum power consumption of each device Defective power cables are not to be used to connect the device and the mains power Only use eluents that have a self ignition point higher than 150 C under normal ambient conditions Only connect devices to voltage sources whose voltage equals the device s voltage Organic solvents are toxic above a certain concentration Ensure that work areas are always well ventilated Wear pro tective gloves and safety
25. d Next steps Replace or clean the check valves 45 Note Installing the check valve Legend Check valve Ball dotted line Flow direction arrow Maintenance and Care Cleaning the Check Valve The check valves are not disassembled for cleaning but they are cleaned as a unit 1 Put the valve in a beaker with solvent e g isopropanol 2 Put the beaker in an ultrasonic bath for at least 10 minutes Installing the Check Valve The ball and position of the valves have been harmonized to each other Insert the valves in the direction of flow Process 1 Insert the check valves iD 2 Manually screw in inlet and outlet fittings and tighten them with a torque wrench and the respective torque Fig 42 BCheck valveall valve Torques for Inlet and Outlet Fittings Pay attention to the torques when tightening inlet and outlet fit tings Pump head stainless Torque steel Cleaning and Caring for the Device CAUTION Intruding liquids can cause damage to the device Place solvent bottles next to the device or on a solvent tray Moisten the cleaning cloth only slightly All smooth surfaces of the device can be cleaned with a mild commercially available cleaning solution or with isopropanol 46 Technical Data Technical Data Conveying sys Dual piston pump tem Flow rate range 100 ml pump head 0 1 100 ml min 250 ml pump head 0 1 250 ml min 500 ml pump head 0 1 500 ml
26. d and consecu tively pull out all Allen screws Remove the pump head Intermediate result The pump head is dismounted To mount the pump head pro ceed the steps in reverse order Next steps Mounting a pump head Attaching Capillaries to the Pump Head Tools Open end wrench sizes 10 17 1 Slide the fitting onto the capillary 2 Slide the clamping ring onto the capillary so the capillary comes out Screw fitting 44 Maintenance and Care Using an open end wrench hold the inlet fitting in place Tighten the fitting Y at the pump head If the capillary cannot be screwed tight then use a new clamping ring Fig 40 Pump head Maintaining the Check Valves Dirty check valves do not properly open and close They cause pressure fluctuations and irregular flow If it is impossible to clean the check valves replace the whole unit Prerequisite Pump head has been flushed Capillaries are disconnected Pump head has been dismounted Procedure Unscrew the outlet and inlet fittings Remove the check valve Insert the check valve Screw in the outlet and inlet fittings Tools Open end wrench sizes 10 17 Removing the Check Valves Process Unscrew the outlet fit ting Remove the check valve OF Unscrew the inlet fit ting Remove the check valve Fig 41 Removing the check valve Intermediate result Check valves are remove
27. e saer ena aio e da 21 Connecting the Eluent Line to the Pump Head 22 Ventingiihe PUMP sets overt cone ia sr ea 23 Using PEER ELUNOS asaru nare Er eE eE yA e adas 23 keak Management exar e ire 24 Connecting the Leak Management 2 ce eee eee eee 24 Setting the Leak SENSO lt 2 otavetudbassed reneteoreneraneanee ened 25 Deactivating the Leak Sensor eee eee eee teens 25 EMUIMINAUNG the Leak sora oa oe eas Se he ook ake ee aoe Sen 25 V6840A 4 Table of Contents HONG Bracket rca rada porras 26 Attaching a Holding Bracket to the Side Panel 27 Mounting a KNAUER Valve o o o o ooooocooooocoooo ooo mooo o 28 Mounting a VICI Valco Valve ooooooooooooooo en ene 29 Mounting a VICI Valco valve drive oo oooooocooooooooooo 30 Mountngia FIOW Cell 22564 cata circa 31 Mounting the Axially Compressible Column Vertex Plus AX 20 mm 30 mm 31 Control the Devices with a Computer in a Local Area Network LAN 32 Contiguring the LAN Setungs 22406 40see euseeree ad te oe eee eed 32 Connecting the CabDles unes osas ainia cierra cr 32 Contiguring tie ROUE 0 er is E A 33 Integrating the LAN into a Company Network o o ooo oo ooo 33 Controlling Several Systems Separately ina LAN 34 Troubleshooting for Connection Problems 2 00 eee eee 34 Switching On the PUMP siri 24 2020 234658 28 deer OE e 36 P
28. ee workdays 57 Environmental Protection Environmental Protection AVV marking WEEE registration Disposal Drop off old devices at the certified waste facilities where they will be disposed of properly According to the German Abfallverzeichnisverordnung AVV January 2001 old devices manufactred by KNAUER are marked as waste electrical and electronic equipment 160214 KNAUER as a company is registered by the WEEE number DE 34642789 in the German ElektroAltgerateRegister EAR It belongs to category 8 under which fall all medical devices and laboratory equipment Within the meaning of the WEEE directive all distributors and importers are responsible for the disposal of old devices End users can send their old devices which must have been manu factured by KNAUER back to the distributor the importer or the company free of charge but would be charged for their dis posal 58 Declaration of Conformity Declaration of Conformity Manufacturer name and address Pump P 2 1L Wissenschaftliche Geratebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Product number EPE20 The device complies with the following requirements and prod uct specifications DIRECTIVE 2006 42 EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 17 May 2006 on machinery and amending Directive 95 16 EC recast IEC 60799 1998 Electrical accessories Cord sets and inter connection cords IEC 6
29. em The drainage system ensures that escaping liquids flow into a waste bottle If there is to much liquid the red LED starts flashing Both device and data acquisition from the chro matography software are stopped m Eu v FYA121400002 6PJA e 3 wv d La y ee Fig 18 View of the drainage system Connecting the Leak Management Front panel has been removed Process 1 Carefully push the fun nel into the center opening of the capillary guide Fig 19 Funnel and capillary guide 2 Push the long ending of the first nozzle into the hose Fig 20 Hose and nozzle 25 Initial Startup Next steps Prerequisite Note When should you deacti vate the leak sensor Prerequisite Procedure Process Connect the nozzle and the funnel Push the other end of the hose onto the noz zle of the leak tray _ Vay pa Ys _ A MN For the bottom device push the short end of the nozzle into the opening in the collec tion point of the leak tray Fig 21 Hose connected to Connect the hose to device the nozzle and lead the second ending to the waste bottle Place the waste bottle below the bottom device Fig 22 Leak tray with nozzle Place front on the device Setting the Leak Sensor The leak sensor is preset to medium The sensitivity can be adjusted in 3 steps low medium high Device
30. ering substances It can alterna tively be used as a single module The pump transports the mobile phase within the chromatography system For transpor tation there are two operating modes possible Standard mode Fluid transportation with a flow rate of up to 1000 ml min Dosing mode Fluid transportation with a dose flow In laboratories the device can be used in the following areas Separation of chiral substances Purification of biomolecules Purification of fine chemicals Purification of active pharmaceutical ingredients API Features The pump transports the fluid By choosing the pump head accordingly it is possible to reach the following maximum val ues Pressure up to 400 bar at a 100 ml min flow rate Flow rate of 1000 ml min at a 50 bar pressure P 2 1L offers the following features Self priming pump Prolonged operating time because of the automatic piston backflushing 7 Options Intended Use Operating mode Isobar The pump transports liquid at a con stant pressure Leak management KNAUER offers the following accessories Stainless steel pump heads Titanium pump heads for biocompatible applications Display control Heating cooling elements for the pump head Valve block for binary or ternary low pressure gradients Pump Heads The pump automatically recognizes the pump head by means of the RFID chips The pump head is equipped with an RFID chip It is used to monitor and sa
31. fication as chemical laboratory technician or comparable vocational training The following knowledge is required Fundamental knowledge of liquid chromatography Knowledge regarding substances that are suitable only to a limited extent for use in liquid chromatography Knowledge regarding the health risks of chemicals If you do not belong to this or a comparable professional group under no circumstances may you perform the work described in this user manual What must be taken into All safety instructions in the user manual account The environmental installation and connection specifications in the user manual Observe national and international regulations pertaining to laboratory work Original spare parts tools and eluents made or recom mended by KNAUER Good Laboratory Practice GLP For development of methods and validation of devices Pro tocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory American Journal of Medical Technol ogy 44 1 pages 30 37 1978 Accident prevention regulations published by the accident Insurance companies for laboratory work More safety relevant information is listed in alphabetical order in the following table Decontamination Contamination of devices with toxic infectious or radioactive substances poses a hazard for all persons during operation repair sale and disposal of a device All contaminated devices must be properly de
32. he eluent inlet fitting of Fig 15 Cutting ring with Tef the pump head lon hose 3 Tighten the fastening screw by hand 4 Plug a blind fitting into the unused inlet Fig 16 Inlets of the valve block Intermediate result Valve block is mounted to the pump head Next steps Connect the plug of the valve block 23 Initial Startup Venting the Pump Prerequisite Capillary connections are connected Before the pump can be used it must be vVentented Tools Syringe CAUTION Damage to the columnn due to venting Open the venting screw or remove column Open the venting screw of the pressure transducer 2 With the syringe extract fluid through the venting port If the fluid flows contin uously stop suction Fig 17 Venting screw of the and close the venting pressure transducer screw Result No air bubbles in the pump head and in the capillaries Using PEEK Fittings PEEK fittings can withstand pressures up to 400 bar for 1 16 and 200 bar for 1 8 CAUTION Damage to the pump head caused by strongly tightened capillary fittings Check the torque of screw fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings Funnel Capillary Nozzle Nozzle 24 guide Collection point with leak Leak tray sensor Prerequisite Procedure Initial Startup Leak Management The leak management consists of the leak sensor and the drain age syst
33. l as the flow rate 38 Operation Operation A device can be operated in two ways Control with chromatography software Control with Control Unit Control with Chromatography Soft ware To control the device with chromatography software it must be connected to the computer through the LAN interface AZURA Devices can be controlled with e g ChromGate version 3 3 2 or higher and ClarityChrom version 3 0 7 or higher You find a detailed description on the chromatography software in the software manual Control with Control Unit You can control the device using the touchscreen on the mobile Control Unit The Control Unit is an optional accessory You find a detailed description on the Control Unit in its accompanying user manual Meaning of the LEDs There are three LEDs and a switch on the front of the device Legend Left LED Center LED Right LED Switch Fig 36 LEDs and switch on the front of the device The LEDs can have different colors depending on the operating conditions Standby To activate the standby keep the switch pressed for 5 seconds 39 Operation Note Malfunctioning system after repeated standby possible After repeatedly using the standby switch off the power switch and back on again to reset the data storage Operating condi Operation tion Left LED red Error message Check the sys tem Shortly press the switch to deacti vate the error message
34. le in isocratic the gradient in the program or in mode setup No link available Create a link and edit it No link available Pls Create a link and edit it edit link first No time table to Edit the data by means of the chroma start tography software Non existing com Switch on the channel or edit the data ponent is set to non using the chromatography software O value P Program does not Create a program exist Program is running Quit program or wait until program has been completed Program not com Modify the program or replace the patible with pump pump head head S Sum of components Change the entry is not 100 T This link is used in First quit or delete wakeup program WAKEUP wu Wake Up then edit or delete link This program is used First pause or delete the link then edit in a link or delete data by means of the chro matography software This program is used First quit or delete wakeup program in WAKEUP wu Wake Up then edit or delete data by means of the chromatography software Time already exists Correct the time entry 55 Troubleshooting Too many lines in Check the number of program lines program A maximum of 100 program lines are possible U Unable to attain Confirm pump continues running min flow set point Unknown pump Check the pump head head type Check whether a pump head with RFID recognition has been installed W Wake up time Correct the entry f
35. le loops Mounting at the holding bracket 11 ml and 40 ml by using the adapter 27 Initial Startup Attaching a Holding Bracket to the Side Panel Before accessories can be mounted the holding bracket has to be attached to the side panel Tools Allen screwdriver size 2 5 Process Procedure 1 Position the holding bracket onto the bore holes in the side panel 2 Using the wrench fasten the screws Abb 24 Holes Abb 25 Screws holding bracket Result Holding bracket is attached Next steps Mount accessories like valves flow cells or columns to the hold ing bracket 28 Initial Startup Mounting a KNAUER Valve Prerequisite Holding bracket is attached to the side panel Tools Allen screwdriver size 3 Process Procedure 1 Position the valve on the front side of the holding bracket Fasten the valve with two screws and two washers from the back side Abb 27 Rear view Result KNAUER valve is mounted 29 Initial Startup Mounting a VICI Valco Valve Prerequisite Holding bracket is attached to the side panel Tools Allen screwdriver size 2 5 Tacas ee Procedure 1 Position the adapter plate O onthe rear side of the holding bracket 2 Slide the valve and the adapter plate through the hole of the holding bracket 3 Fasten the valve with four screws to the front side Abb 28 Rear view Abb 29 Front view Result KNAUER valve is mounted
36. min 1000 ml pump head 0 1 1000 ml min Maximum pres 100 ml pump head Ure 400 bar to 100 ml min 250 ml pump head 225 bar to 100 ml min Linear reduction 225 200 bar of 100 150 ml min 200 bar of 150 250 ml min 500 ml pump head 100 bar to 500 ml min 1000 ml pump head 75 bar to 350 ml min Linear reduction 75 50 bar of 350 600 ml min 50 bar of 600 1000 ml min Flow rate accu 2 at 2 50 of the flow range with racy methanol water mixture 10 90 v v Flow rate preci RSD Relative Standard Deviation sion lt 0 1 Gradients Isocratic HPLC pump Pump with binary or ternary LPG valve block low pressure gradient sys tem LPG Up to 4 pumps combined high pres sure gradient system HPG System protection Pin and Pmax adjustable Operation LAN Pin header connectors Analog IN Start IN Error IN Control Unit 47 Technical Data Programming 19 programs 9 program links Links WAKE UP program Supply voltage Pump 100 240 V range Binary LPG valve block 24 V Ternary LPG valve block 12 V Supply frequency 47 63 Hz Power consump Pump maximum 320 W tion Binary or ternary LPG valve block 5 W IP protection class IP 20 Dimensions with 397 x 242 x 201 mm pump head length x width x height 48 Accessories and Spare Parts Accessories and Spare Parts For repeat orders of spare parts use the enclosed packing list Contact the Technical Support in
37. n tightly If the router is integrated into a company network pull out the patch cable from the WAN port Can the devices communicate with the computer even though the router is disconnected from the com pany network 35 Initial Startup In case you own a Control Unit check the settings in the menu Setup gt Network Is LAN DHCP set for controlling Did the device receive an IP address Turn off all devices router and computer Firstly turn on the router secondly the devices and the computer Has this been successful Replace the patch cable to the device with that no connection could be established Has this been successful The pump stops automatically when the communication between pump and software is interrupted for longer than 10 s Possible reasons for interrupted communication Software or operating system crash Network problems 36 Prerequisite Switching on the pump Result When is flushing required Note Initial Startup Switching On the Pump CAUTION Damage to the pump head in case it runs dry Ensure that liquids runs through pump head and piston backflushing Liquid container is sufficiently filled Piston backflushing is connected Washing container is sufficiently filled Process 1 Switch on the pump at the power switch on the rear side 2 Wait until the pump has completed the self test If the self test has been successf
38. o inlet screw fittings of the pump head Legend Knurled head screw Eluent inlet Olive type tube fit ting Fig 6 Eluent inlet for pump heads from 100 500 ml Fig 7 Eluent for 1000 ml pump head Connecting the eluent inlet 1 With the knurled head screw screw the elu ent inlet into the inlet screw fitting of the pump head Turn the eluent inlet until the intake mani fold input points for ward Fig 8 Eluent inlet variant 1 Tighten the knurled head screw 20 Initial Startup Connection of the Eluent Line Prerequisite The eluent inlet is connected Procedure Connect the eluent line to the pump head Note If connecting a pump head from 100 500 ml make sure that the tapered side of the cutting ring is pointed towards the fas tening screw of the Teflon tube line Pump head Push the Teflon hose 100 ml through the fastening 250ml screw and the cut 500mI ting ring Insert the hose end as far as possible into the eluent inlet fitting Y of the pump head Tighten the fastening screw by hand Fig 9 Connecting the eluent line to the pump head Connecting the eluent line Pump head 1 Push the Teflon hose 1000 ml directly on the olive type tube fitting 2 Fasten the Teflon hose with hose clamp Fig 10 Eluent line and 1000 ml pump head Result The eluent line is connected Next steps Check the seal and lines of the connections 2
39. or date or otherwise already passed time 56 Legal Information Legal Information Warranty Conditions The factory warranty for the device is valid for 12 months after the date of dispatch All warranty claims shall expire in the event that any unauthorized changes are made to the device During the warranty period any components with material or design related defects will be replaced or repaired by the manu facturer free of charge This warranty excludes the following 1 Accidental or willful damage 2 Damage or errors caused by third parties that are not con tractually related to the manufacturer at the time the damage occurs 3 Wear parts fuses glass parts columns light sources cuvettes and other optical components 4 Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5 Packaging and transport damage In the event of device malfunctions directly contact the manu facturer Manufacturer Wissenschaftliche Geratebau Dr Ing Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 E Mail info knauer net Internet www knauer net Transportation Damages The packaging of our devices provides the best possible protec tion against transport damage Check the devices for signs of transportation damages In case you notice any damage con tact the technical support and the forwarder company within thr
40. ordered separately from the manufac turer 4 Maintenance and Care Maintenance and Care Contact data Technical Support European hotline E mail contact Proper maintenance of the HPLC device will ensure successful analyses and reproducible results Switching Off the Pump If you want to switch off the pump for a longer term flush the pump head with isopropanol Contact with the Technical Support If you have any technical questions regarding the hardware or software of the manufacturer please use one of the contact options below Technical Support Hotline Languages German and English Available by telephone 8 am to 5 pm CET Phone 49 0 30 809727 111 Fax 49 0 30 8015010 support knauer net manufacturer Maintenance Contract The following maintenance work on the device may only be per formed by the manufacturer or a company authorized by the manufacturer and is covered by a separate maintenance con tract e Opening the device or removing housing parts What maintenance tasks can users per form on the device CAUTION Maintenance tasks on a switched on device can cause damage to the device Switch off the power switch and pull the power plug Users may perform the following maintenance tasks themselves Replacing the pump head Replacing the check valves of the pumps 42 Legend O Capillary screw fit ting Allen screws Outlet fittings Inlet fittings Eluent inlet
41. ple Designation Flushing solution Connecting the piston backflushing Initial Startup Connecting the Piston Backflushing The piston backflushing removes salts and other substances from the area behind the seals To do this connect a bottle with flushing solution to the flush pump and to the pump head The connection between pump head and flush pump is preinstalled at the factory The piston backflushing function automatically flushes the rear piston area of the pump head upon switch on and in continuous mode Upon switch on The rear piston area of the pump head is automatically flushed for 30 seconds In continuous mode The rear piston area of the pump head is flushed automatically every 60 minutes for 30 seconds Inlet and outlet of the flush pump are located on the front of the device A Y Fig 4 Inlet symbol Fig 5 Outlet symbol These are the recommended flushing solutions Water Mixture of 80 water and 20 ethanol Isopropanol o 1 Use the first hose to connect the inlet of the flush pump to the bottle containing the flushing solution 2 Use the second hose to connect the pump head to the bottle containing the flushing solution 19 Initial Startup Eluent Inlet The eluent lines are connected by the eluent inlet to the pump head Before the eluent lines are connected the eluent inlet must be attached to the pump head Procedure Fasten the eluent inlet to the tw
42. rately you can use different ports to avoid interference Therefore the port number for each device must be changed and this same number must be entered into the device configu ration of the chromatography software We recommend to use the same port number for all devices in the same system The port is set to 10001 at the factory You must use the same numbers in the device configuration of the chromatography software as in the device otherwise the connection fails 1 Change the port number of the device 2 Enter the port number in the chromatography software The connection is established Troubleshooting for Connection Problems In case no connection between the computer and the devices can be established go through the following points Check after each point if the problem is solved If you did not manage to locate the problem call the Technical Support 1 Check the status of the LAN connection in the Win dows taskbar hal Connected fan Connection not established If no connection was established test the following Is the router on Is the patch cable connected correctly to the router and the computer Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices Check all connections Are the patch cable connected to the LAN ports and not the WAN port Are all cable connections between devices and router correct Are the cables plugged i
43. s power system Configuring the Router The router is preset at the factory You can find a label at the bottom side of the router on which IP address user name and passwort are printed These information help to open the router configuration 1 To open the router configuration start your Internet browser and enter the IP address not for all routers 2 Enter user name and passwort 3 Configure the router as DHCP server 4 In the router configuration check the IP address range and make changes if necessary Once the router has assigned IP addresses to all devices the chromatography software can be used to remotely control the system Integrating the LAN into a Company Network A network administrator can integrate the LAN into your com pany network In this case you use the WAN port of the router There is a patch cable for the connection 1 Check that the IP address range of the router and of the com pany network do not overlap 2 In case of an overlapping change the IP address range of the router 3 Use the patch cable to connect the router WAN port to the company network 4 Restart all device including the computer 34 Note Procedure Result Initial Startup Controlling Several Systems Separately in a LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one HPLC system is con nected to the same LAN and you plan on controlling them sepa
44. s itself The system messages are in alphabetical order A Auto pump head Switch the device off and on type head data Check whether a pump head with uninitialized RFID recognition has been installed Repeat the automatic configuration step in the chromatography soft ware Remove pump head clean it and install it again Auto pump head Switch the device off and on type no valid head Check whether a pump head with detected RFID recognition has been installed Repeat the automatic configuration step in the chromatography soft ware Remove pump head clean it and install it again Auto pump head Pump head without RFID detection If type RFID hardware necessary replace pump head not present or failed 52 Troubleshooting System message Auto pump head type read failed Auto pump head type write failed C Cannot edit pro gram from the run ning link Cannot delete active program link Cannot edit pro gram from the run ning link Switch the device off and on Repeat the automatic configuration step in the chromatography soft ware Remove pump head clean it and install it again Inform the Technical Support of the manufacturer in case the sys tem message repeats itself Switch the device off and on Repeat the automatic configuration step in the chromatography soft ware Remove pump head clean it and install it again Inform the Technical Support of the manufacturer in case the
45. ter 33 Settings 32 Setup 32 Troubleshooting 34 Leak management Connecting 24 Deactivate leak sensor 25 Setting Leak sensor 25 Leaks Capillary screw fittings 42 Local Requirements 13 M Maintenance Maintenance contract 41 Mandatory signs 11 Manufacturer 56 Modifiers 8 O Operation Qualification 40 OQ 40 P Power supply 10 13 Power cable 10 Power strip 10 Pump head 42 Remove 42 Pump heads types 49 R Remote control see spring strip 18 S Salts 8 Screw fittings Tighten 42 Solvents 8 Spare parts 12 Supply voltage see power supply 13 61 Index y Technical Support 13 41 Test intervals 40 Transportation damages 56 Troubleshooting Troubleshooting 50 V Valve 29 30 W Warranty conditions 56 Warrning signs 11 Wissenschaftliche Ger tebau Dr Ing Herbert Knauer GmbH All rights reserved The information in this document is subject to change without prior notice Translation of the original German edition of this manual 2012 10 31 Printed in Germany on environmentally friendly paper from sustainable forests AZURA are registered trademarks of Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH See up to date manuals online www knauer net downloads HPLC SMB Osmometry Wissenschaftliche Geratebau Phone 49 30 809727 0 Dr Ing Herbert Knauer GmbH Telefax 49 30 8015010 Hegauer Weg 38 E Mail info knauer net 14163 Berlin Germany Internet www knauer net www
46. ully com pleted the LED on the Fig 35 Power switch right lights up green The pump is now ready for operation If the test fails an error message will be displayed Contact the Technical support of the manufacturer if the error occurs several times Flushing the Pump To flush the pump insert the inlet hoses into the storage con tainers and start the pump with an intermediate flow rate As the pump is self priming the venting screw can be open CAUTION Damage to the columnn due to venting Open the venting screw or remove column Flush the pump in the following cases At initial startup to eliminate air bubbles in hoses and capillar les When changing solvents After using buffer solutions to eliminate salt residues Use the solvent for flushing that is to be used in the subsequent application If you used a buffer solution pay attention to choosing a solvent for flushing in which the buffer solution is soluble The purging process of the pump is limited to a maximum pres sure of 5 MPa If this value is exceeded during the purging pro cess the pump switches off automatically If you are using very small hoses and capillaries the pressure can be too high 37 Initial Startup How long does flushing If there are air bubbles in the capillaries the flow pulsates As take soon as the flow is constant the pump is vented and flushing can be stopped The duration for flushing depends on capillary and hose length as wel
47. ve all important parameters and set tings of the pump and pump head Pump head for use in preparative applications Standard model stainless steel Pump heads with titanium for biocompatible applications 100 ml 250 ml 500 ml 1000 ml 8 Note Suitable eluents Acetone Acetonitrile Benzene Chloroform Ethyl acetate Ethanol Hexane heptane lsopropanol Carbon dioxide liquid 99 999 CO gt Methanol Phosphate buffer solu tions 0 5 M Toluol Dilute ammonia solu tion Dilute acetic acid 10 50 at 25 C Dilute sodium hydrox ide 1M Water Intended Use Eluents Even small quantities of other substances such as additives modifiers or salts can influence the durability of the materials The list of selected solvents was compiled based on research in the pertinent literature and is only a recommendation If there is any doubt contact the technical support of the manufacturer Less suitable eluents Not suitable eluents Dimethyl sulfoxide DMSO Slightly volatile eluents Methylene chloride Tetrahydrofuran THF Dilute phosphoric acid Halogenated hydrocar bons e g Freon Concentrated mineral and organic acids Concentrated bases Eluents containing parti cles Perfluorinated eluents e g Fluorinert FC 75 FC 40 Perfluorinated polyether e g Fomblin 9 Safety for Users Safety for Users Professional group The user manual is addressed to persons that have quali
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