Magnesia 16 Genomic DNA Whole Blood Kit
Contents
1. f m gt m a sisislslF slg gl eg KE 9 S 5 ue a A a 5 RS RS gt o gt a s H 23 23 S S Y x S gs gs 8 8 E 551515 85 3 s T amp amp a S Sc Sc gt m m S m ni Q S 53 53 SI zn gsm 8 28 R 1 Description When it comes to automated viral extraction the Magnesia Viral Nucleic Acid Extraction kit and Magnesia 16 System should be your first choice With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes both DNA RNA and DNA RNA virus can be extracted using the same kit in afastand economical way Applications For general laboratory use The purified total nucleic acid is suitable for highly sensitive and quantitative PCR Magnesia Viral Nucleic Acid Extraction Kit has been performed and proved with HBV HCV HIV and influenza viruses as downstream applications n www anatoliageneworks com Magnesia 16 Running Time 45 min sample volume 200 ul 56 min sample volume 400 ul Preparation before using 1 Add 1 0ml RNase Free Water to the Carrier RNA tube and mix by vortexing Store prepared Carrier RNA 1mg ml at2. Confirm your input code again and press Enter to next page for sample volume selection Magnesia 16 NA 000 000 Oul 2 000ul PREV PAGE coag Helle Select Sample Volume v CARTRIDGE CODE 000 000 Enter NEXT PAGE ESC CANCEL Confirm yourinput Sample Volume Press Enter for next page Press ESC for back to Stand By page Dee ej e BA m a e Atthis step prepare racks to operation area After racks loaded then press Enter to next page for elution volume selection v T ELUTE VOL Oul 2 000ul Oul 4 0001ul PREV PAGE Selectfinalelution volume v x 0 ELUTE VOLUME 000 PREHEATING Tmp0000 290 G Z Eee v Magnesia 16 in process of selected protocol at this step The Green Indicat LCD lights up and Heating Block starts to heat up to 65 Cfor Lysis Step During Magnesia 16 isunderprogramrunning the Magnesia LCD lights up at all times DONOT open the door at this moment it causes emergent stop and you might lose your samples by machine interruption WBB PROCEDURE COMPLETED lE 4 5 6 START CONTUNUE 7 8 9 STOP STAND BY OAD Esc While program finished beep sound can be heard and green Indicate LCD
3. Description Magnesia 16 Genomic DNA Whole Blood Kit has been designed for purification of total DNA including genomic mitochondrial and viral DNA from whole blood plasma serum buffy coat by using automated instrument of Magnesia 16 The method uses pre filled cartridge contains proteinase K and a chaotropic salt guanidine hydrochloride to lysis cells and degrade protein DNA in chaotropic salt binds to cellulose coated magnetic beads After washing off the contaminants the purified DNA is eluted by low salt elution buffer or water Purified DNA of approximately 20 30 kb in length is suitable for PCR or other enzymatic reactions Applications For general laboratory use Using magnetic particle technology to purified genomic DNA from fresh whole blood The purified genomic DNA can be directly used for downstream application like quantitative PCR restriction enzyme digestion southern bloting www anatoliageneworks com Magnesia 16 Running Time 44 min sample volume 200 ul 57 min sample volume 400 ul Whole Blood Protocol Pipet 200 400ul of equilibrated whole blood sample to a microcentrifuge tube then put the Sample Tube into hole 4 of T Rack Buffy Coat modify Protocol Anatolia Lysis Buffer 150mMNH C 10mM KHCO 0 1mM EDTA Method 1 Anatolia Lysis protocol Take 600 700ul whole blood into 2ml microcentrifuge tube Don t take more than 700ul whole blood sample it will cause the leakage sit
4. 02 sees HH HH III hA M 19 Running HJ HUGE IL IIl 070 2000000 0002 21 IVD CE Forinvitro diagnostics with Magnesia Kits Bosphore Kits and Montania 483 System Precautions 1 Handling Requirements Do notusea kit after its expiration date has passed Some reagents contain the hazardous compounds guanidine thiocyanate or guanidine hydrochloride Do not let these reagents touch your skin eyes or mucous membranes If contact does occur wash the affected area immediately with large amounts of water If you spill the reagents dilute the spill with water before wiping it up Do not allow reagents containing guanidine thiocyanate to mix with sodium hypochlorite solution or strong acids This mixture can producea highly toxic gas Il Laboratory Procedures Handle all samples and the resulting waste as if potentially infectious using safe laboratory procedures As the sensitivity and titer of potential pathogens in the sample material varies the operator has to optimize pathogen inactivation by the Lysis Buffer or take appropriate measures according to local safety regulations Anatolia Inc does not warrant that samples treated with Lysis Buffer are completely inactivated and non infectious After sample processing is completed remove and autoclave all disposable plastics if you worked with potentially infectious sample material Do noteat drink or smoke in the laboratory work area Do not pipette b
5. Elution Eppendorf Tube Elution Eppendorf Tube GT Buffer 30ml GT Buffer 30m Filter Column Filter Column Proteinase K 11mg PK Storage Buffer 1 25 Proteinase K 11mg PK Storage Buffer 1 25ml Storage and Stability 1 The kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer Cartrige Contents m m a gt m 3 2181218121921 15 gs s s s 5 52 a s amp amp S U sg sg 5 NS g Sm Sa 2 s th xx 8 8 2 D ki SR 5 x ES S S S 4 Q S S gt E S m S E S Ss 9 3 3 z Sa Ta S P x E elli Description Magnesia 16 Genomic DNA Tissue Kit has been designed for purification of total DNA including genomic mitochondrial and viral DNA from a variety of animal tissues or cells by using automated instrument of Magnesia 16 The provided Filter Column Set can filtrate hard tissue sample or swab sample to prevent tissue residues to obstruct pipette syringe during the process of Magnesia 16 The method uses pre filled cartridge contains proteinase Kand a chaotropic salt guanidine hydrochloride to lysis cells and degrade protein DNA in chaotropic salt binds to cellu
6. Nucleic Acid Extraction Kit User s Manual CONTENTS Precautions exi s Fa e 1 Howto Use Kit 0000 2 Introduction of each Magnesia 16 Nucleic Acid Extraction kit Kit Contents Description Applications pretreatment Protocol Magnesia 16 Genomic DNA Whole Blood Kit Speedy installation Cartridge Code 101 Cat NoMGB400 01 MGB400 02 e Hee me e ne eene 3 Magnesia 16 Genomic DNA Whole Blood Kit Cartridge Code 102 CatNo MGB400 03 MGB400 04 ehh etie 5 Magnesia 16 Genomic DNA Large Volume Whole Blood Kit Cartridge Code 104 CatNoMGB1200 I II Hh hh hh hh hh 7 Magnesia 16 Cultured cells DNA Kit Cartridge Code 110 CatNoMCC 01 MCC 02 eene nennen nennen enne senes nennen 9 Magnesia 16 Viral Nucleic Acid Extraction Kit Cartridge Code 201 Cat No MVN400 01 MVN400 02 reece nner eee emn 11 Magnesia 16 Viral Nucleic Acid Extraction Kit Cartridge Code 202 CatNo MVNA400 03 MVN400 04 S HII een eee ee eee ee eee hh hh 13 Magnesia 16 Genomic DNA Tissue Kit Cartridge Code 401 CatNoMGT 01 MGT 02 s Hh ee RK R R R 15 Magnesia 16 Genomic DNA Bacterial Kit Cartridge Code 502 Cat No MBB 01 MBB402 sien s n RR IH ERR YERA Za RE LARA RE RR RET EROR EA ends 17 Magnesia 16 Total RNA Whole Blood Kit Cartridge Code 601 CatNoMRN 01
7. atroom temperaturefor 10min 4 Resuspend sample mixture by pipetting Then adding 40ul proteinase 10mg ml to sample mixture and vortex to mix sample Run Code 502 program at Magnesia 16 Magnesia 16 Total RNA Whole Blood Kit For total RNA purification from white blood cells of human whole blood by using Magnesia 16 of Anatolia Inc Cartridge Code 601 Cat No MRN 01 MRN 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MRN 01 Contents Pre filled Cartrige Reagent 36 pcs Pipet Tip 36 PCS Tip Holder 30 PCS Sample Tube masennuin ERE eset 36pcs Elution Eppendorf Tube 36pcs Anatolia Lysis Buffer 100ml REBUfe M 15ml Cat No MRN 02 Contents Pre filled 100pcs Pipet Tip 100pcs Tip Holder Sample Tube Elution Eppendorf Tube Anatolia Lysis Buffer 200ml RRB BUT CP steele tenemus dE 30ml Storage and Stability 1 The kit should be stored at room temperature Cartrige Contents 2 iy Ies T t F 2 g S S a S a n a a S 4 uw S ug
8. 20 C 2 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 4 C Protocol 1 Pipet 5 ul for 200 ul sample or 10 ul for 400 ul sample Carrier RNA 1mg ml into the sample tube and add Proteinase K 10 ul for 200 ul sample or 20 ul for 400 ul sample into the 1 5 ml sample tubes provided 2 Add 200 ul or 400 pl of serum into the sample tubes 1 Placing the 1 5 mlsample tubes into the MagCore T rack Run Code 201 program at Magnesia 16 Introduction of each Magnesia 16 Nucleic Acid Extraction kit Magnesia 16 Viral Nucleic Acid Extraction Kit For extracting Viral DNA RNA from serum plasma cell free body fluids by using Magnesia 16 System of Anatolia Inc Cartridge Code 202 Cat No MVN400 03 MVN 400 04 Kit Contents Check that the following parts are included in addition to the main unit Cat No MVN400 03Contents Cat No MVN400 04 Contents Pre filled Cartrige 4 36 pcs Pre filled Cartrige Reagent Pipet Tip 3 PCS Pipet Tip goce X ETE 36 pcs Tip Holder Nee d 36 pcs Sample Tube Elution Eppendorf Tube 36 pcs Elution Eppendorf Tube Carrier RNA 1mg RNase Free Water 1 25ml Proteinase K 11mq PK Storage Buffer 1 25ml PCs Carrier RNA 1mq IPCs RNase Free Water 1 25 1 PCS Proteinase K 11m
9. 502 Cat No MBB 01 MBB 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MBB 01 Contents Pre filled Cartrige Reagent Pipet Tip Tip Holder Sample Tube Elution Eppendorf Tube Lysozyme Reaction Buffer 15ml Proteinase 11 PK Storage Buffer RNaseA 50mg ml 160 Cat No MBB 02 Contents Pre filled Cartrige Reagent Pipet Tip Tip Holder Sample Tube Elution Eppendorf Tube Lysozyme Reaction Buffer 30ml Proteinase K 11mq PK Storage Buffer 1 25ml RNase A 50mg ml 400 Storage and Stability 1 The kit should be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer 3 Store RNaseA solution at 4 C when kit arrived for long term storage Cartrige Contents 2 m Q JEF s 3 3 S se RR amp s SS 5 o S a S o o gt gt s D ERR ER S 8 815315 3g 3g 3 S g 2 si S 8 8 a s DA a 8 81813 18 N Description Magnesia 16 Genomic DNA Bacterial kit is designed to extract genomic DNA from both Gram and Gram bacteria via automa
10. Nase tube and place the DNase tube into hole 3 of T Rack Healthy Whole blood ul DNase l ul DNase buffer 1X ul Up to 400 3 RNase free DNase lis not including in Magnesiatotal RNA Whole Blood Kit we recommend to use Anatolia RNase free DNase I cat DN36 or cat DN96 for genomic DNA treatment For product information please contact Anatolia Inc distributor We also recommend to use RNase free DNase enzyme 1U ul of Novagen Cat 69182 3 Please contact local Merck branch office or distributor for product information And follow the contents below for buffer preparation 1X DNase reaction buffer 20mM Tris pH8 4 2mM MgCD 50mMkd DEPC water autoclave Fresh Whole Blood Protocol Without DNase treatment 1 Add 1 volume of human whole blood with 3 volumes of Anatolia lysis Buffer in an appropriately sized tube not provided and mix by inversion Do not vortex For example add 1 2 ml of Anatolia lysis Buffer to 400ul of whole blood 2 Incubate the tube for 10 minutes on ice and invert 2 3 times during incubation 3 Centrifuge for 3 minutes at 500x g 2 500rpm at 4 C and completely discard the supernatant 4 Add 500ulAnatolia lysis Bu er to the cell pellet Resuspend cells by vortex briefly 5 Centrifuge for 3 minutes at 500 x g 2 500rpm at 4 C and completely discard the supernatant 6 Add 200ul RB buffer contain 8 ME to the white pellet and mix by vortexing 7 Follow Magnesia 16 process to apply the prepare
11. SE 6 515151 5 SE F F S D s r4 m ERR RR 5 amp S amp amp 8 S N So So E 3 3 S m 8 8 EY 89 s EN amp S S S SO m is 8 8 8 S 8 m BPEL 5 Description Magnesia 16 Total RNA Whole Blood Kit is specially designed for total RNA purification from up to 0 4ml human whole blood by using automated instrument of Magnesia 16 The program provides optional protocol for contaminated genomic DNA remove Combine Anatolia high quality RNase free DNase with Magnesiatotal RNA Whole Blood Kit can provide high quality DNA free total RNA you can get high sensitivity result for downstream application like qRT PCR Applications For general laboratory use Using magnetic particle technology to purified total RNA The purified RNA can be directly used for downstream application like real time PCR RT PCR cDNA synthesis www anatoliageneworks com Magnesia 16 Running Time 42min without DNase treatment starting volume 200 ul 68min with DNase treatment starting volume 200 ul Preparation before using 1 B Mercaptoethanol B ME not provided must be added to RB Buffer before use Add 10ul of B ME per 1 ml of RB Buffer 2 Recommended Step DNA residue degradation Prepare DNase RNase free working solution according to the table below Add 15ul DNase with 185ul DNase reaction buffer 1X 15 units DNase per sample in the D
12. ain unit Cat No MGB400 03 Contents Cat No MGB400 04 Contents Pre filled Cartrige Reagent dO PCS Pipet Typ 100 pcs Jo m 100pcs Sample Tube 100 pcs Elution Eppendorf Tube 100 pcs Proteinase K 11mq 4 PCs PKStorage 2pcs PK Storage Buffev 4 PCs Shelf Life 12 month Shelf Life 12 month Storage and Stability This kit should be stored at room temperature Cartrige Contents m isr mimi i y p zs ox ox 5 8 i i 2 211 99 f is S Z 38 lt S883 a eo D xox xo x x amp S 4 S 2 2 S 1415 a a c Sa un uU z u SIS 8 881881 N E E ARJISE Description Magnesia 16 Genomic DNA Whole Blood Kit has been designed for purification of total DNA including genomic mitochondrial and viral DNA from whole blood plasma serum buffy coat by using automated instrument of Magnesia 16 The method uses pre filled cartridge contains chaotropic salt guanidine hydrochloride to lysis cells and degrade protein DNA in chaotropic salt binds to cellulose coated magnetic beads After washing off the contaminants the purified DNA is eluted by low salt elution buffer or water Purified DNA of approximately 20 30 kb in length is
13. d be stored at room temperature 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer Cartrige Contents m Q gt DB el amp lslsimeig Ss es S S Lb a a a S S NI a a 2 Sa S38 g 8 S Slr Br pg BR SR m S E m s 5 5 g S 94 8 8 3 1 ER ER E e Description Magnesia 16 Genomic DNA Large Volume Whole Blood kit is designed to extract genomic DNA from 1 2ml fresh whole blood via automatic machine Magnesia 16 The kit contains all required reagent and labware for automated purification using magnetic particle technology Reagents are supplied in prefilled cartridges which can be loaded into machine directly without extra work Easy select program code number 104 in Magnesia 16 and combine using Magnesia 16 Genomic DNA Large Volume Whole Blood Kit can perform high quality genomic DNA Applications For general laboratory use Using magnetic particle technology to purified genomic DNA from 1 2ml fresh whole blood The purified genomic DNA can be directly used for downstream application like quantitative PCR restriction enzyme digestion southern bloting www anatoliageneworks com Magnesia 16 Running Time 76 min sample volume 1200 ul Preparation before using 1 Add 1 1mIPK Storage Buffer into the tube of P
14. d incubate at 559C for 5min until all residual ethanol has evaporated 8 Tolysis Step For Swab Tissue Preparation before using Additional Requirments PBS Microcentrifuge Tube 1 Separate the swab cotton form the stick Place the swab into a 2ml microcentrifuge tube Not Provided and add 500ul GT Buffer For Buccal Swab sample donor should not ingest anything for at least 30min prior to sample collection 2 ToLysis Step 2 Lysis Step For Solid Animal Tissue starts from this step 1 Add 400l the provided GT Buffer and 20ul Proteinase K 10mg ml to the tube and mix by votexing Before Initial Use Add 1ml PK Storage Buffer to Proteinase K Tube 11mg and stor at 4 C 2 Incubate at 56 C for 90min until the sample has been completely lysed 3 Incubate the sample lysate at 90 C for 30min 4 If there are any insoluble residues in the tube transfer the supernatant to Filter Column Set and centrifuge at full speed for 5min to get clear tissue solution in the Collection Tube 5 Pipette 400ul of clear tissue solution to Sample Tube then put Sample Tube to Hole 4 of T Rack Optional Step RNA Degradation If RNA Free genomic DNA is required perform this optional step 1 Add4ul RNase A 50mg ml into the sample lysate 2 Incubate the sample at room temperature for 20min Magnesia 16 Genomic DNA Bacterial Kit For purification of genomic DNA from Bacterial by using Magnesia 16 of Anatolia Inc Cartridge Code
15. d sample 200ul volume With DNase treatment 1 Follow step 1 6 of without DNase I treatnent protocol to prepane with Blood cell sample 2 Be sure to place the DNase I tube into the well 3 of T Rack option and Follow Magnesia 16 process to apply the prepared sample 200ul volume Well Elution Tube Tip Tip Holder DNase Sample Tube Running Time List Cat number Product Package Code No Running Time k Magnesia 16 Genomic DNA Whole Blood Kit MGB400 02 Speedy Installation MGB400 03 Magnesia 16 Genomic DNA Whole Blood Kit MGB400 04 MGB1200 Magnesia 16 Genomic DNA Large Volume Whole Blood Kit sample volume 200 ul sample volume 400 ul sample volume 200 ul sample volume 400 ul 76 min sample volume 1200 ul 110 44 min sample volume 200 ul up to 5x 10 cells 601 45 min sample volume 200 ul 56 min sample volume 400 ul 57 min sample volume 200 ul 66 min sample volume 400 ul MVN400 01 u Magnesia 16 Viral Nucleic Acid Extraction Kit MVN400 02 MVN400 03 2 7 Magnesia 16 Viral Nucleic Acid Fxtraction Kit MVN400 04 MGT 01 1222 Magnesia 16 Genomic DNA Tissue Kit 33 min sample volume 400 ul MBB 01 MBB 02 Magnesia 16 Genomic DNA Bacterial Kit 44 min sample volume 200 ul Magnesia 16 Total RNA Whole Blood Kit min without DNase 1 treatment MRN 02 HEM 68min with DNase 1 treatment s iar s d s www anatolia
16. disturb the cell pellet Continue with machine application step 1 Machine Application 1 Resuspend cell pellet with PBS Buffer to a final volume of 200 ul 2 Transfer cell mixture 200 ul and add 20 ul Proteinase K into the 1 5 ml sample tubes provided 3 Placing the 1 5 ml sample tubes into the Magnesia T rack Run Code 110 program at Magnesia 16 Introduction of each Magnesia 16 Nucleic Acid Extraction kit Magnesia 16 Viral Nucleic Acid Extraction Kit For extracting Viral DNA RNA from serum plasma cell free body fluids by using Magnesia 16 System of Anatolia Inc Cartridge Code 201 Cat No MVN400 01 MVN 400 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MVN400 01 Contents Pre filled Cartrige Reagent 36 pcs Pre filled Cartrige Reagent Pipet Tip 36 pcs Pipet Tip Tip Holder 36 pcs Tip Holder Sample Tube 36 pcs Sample Tube Elution Eppendorf Tube 36 pcs Elution Eppendorf Tube Carrier RNA 1mq m IPCS Carrier RNA 1mq RNase Free Water 1 25ml Cat No MVN400 02 Contents RNase Free Water 1 25ml a IPCS Proteinase K 11mq PK Storage Buffer 1 25ml Proteinase K 11mg PK Storage Buffer 1 25ml Storage and Stability This kit should be stored at room temperature Cartrige Contents
17. g PK Storage Buffer 1 25ml Storage and Stability This kit should be stored at room temperature Cartrige Contents gt 10 es ez S S a a D D a a amp gt s s gt gt e gt c Ss SS 3S Q S S E zh Xo xox Q E 5 E oo oo E zh Q Q zh zh s 5 38 x a S 8 9 S 2 S 8 e A S g ca sie N m m E x Description When it comes to automated viral extraction the MagnesiaViral Nucleic Acid Extraction kit and Magnesia 16 System should be your first choice With all the kit components of plastic consumables are DNase RNase Free pretreated and individual processing track for each loaded samples this system eliminates all possible cross contamination between samples Built in protocol with flexibility in sample source volumes both DNA RNA and DNA RNA virus can be extracted using the same kit in a fast and economical way Applications For general laboratory use The purified total nucleic acid is suitable for highly sensitive and quantitative PCR MagnesiaViral Nucleic Acid Extraction Kit has been performed and proved with HBV HCV HIV and influenza viruses as downstream applicati
18. geneworks com Anatolia ve Biyoteknoloji A S Kasap Ismail Sokak No 10 23 34722 Kadikoy stanbul TURKEY Tel 90 216 33004 55 www anatoliageneworks com
19. light went out Introduction of each Magnesia 16 Nucleic Acid Extraction kit Magnesia 16 Genomic DNA Whole Blood Kit speedy instalation For purification of genomic DNA from human whole blood by using Magnesia 16 of Anatolia Inc Cartridge Code 101 Cat No MGB400 01 MGB400 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MGB400 01 Contents Cat No MGB400 02 Contents Pre filled 36pcs Pre filled Cartrige Reagent 96 pcs Pipet Tip 36 PCS Pipet TI 100 pcs Tip Holder 30 PCS Tip Holder 100 pcs Sample Tube tia 36 pcs Sample 100 pcs Elution Eppendorf Tube 30 PCS Elution Eppendorf Tube 100 pcs Shelf Life 6 months Shelf Life 6 months Storage and Stability This kit should be stored at room temperature Cartrige Contents 012 gigi ES ox S 8 ae eee ae 1 le 8 8 18 S 1512 Sa 88 S gt EE 48 88 s 8 RE F E B 85 Ba 5055 a amp S N SI sls S s 22 s S S S S E NS EARLE 12
20. lose coated magnetic beads After washing off the contaminants the purified DNA is eluted by low salt elution buffer or water Purified DNA of approximately 20 30 kb in length is suitable for PCR or other enzymatic reactions Applications Animal tissues paraffin embedded tissue swab and blood stain www anatoliageneworks com Magnesia 16 Running Time 33 min sample volume 400 ul Preparation before using 1 Add 1 1mIPK Storage Buffer into the tube of Proteinase Kand mix by vortex Store prepared Proteinase K 10mg ml at 20 C For Paraffin Embedded Tissue Preparation before using Additional Requirments Xylene Microcentrifuge Tube 1 Slice small section 5 10 of paraffin embedded tissue and transfer to a microcentrifuge tube Discard the first 2 3 sections if the surface of paraffin sample has been exposed to air Add Iml xylene to the tube and votex vigorously for 10sec Then Incubate at 60 C for 10min Centrifuge at full speed for 3min at room temperature Remove the supernatant carefully by pipetting then add 1ml ethanol 96 100 to the pellet and mix by vortexing for 10sec Centrifuge at full speed for 5min at room temperature OQ KR t Remove the supernatant carefully by pipetting then add again of 1ml ethanol 96 100 to the pellet and mix by vortexing for 10secto wash again Then centrifuge atfull speed for 5min 7 Removeresidual ethanol with a fine pipette tip then open the tube an
21. of Proteinase Kand mix by vortex 2 Ensure PBS buffer have been prepared for resuspend cell pellet Store prepared Proteinase K 10mg ml at 20 C Protocol Sample Preparation A Cells grown in suspension Cells grown in suspension up to 5 x 10 cells Determine the number of cells Centrifuge the appropriate number of cells for 5 min at 300xg ina 1 5 ml microcentrifuge tube not provided Remove the supernatant completely and discard Continue with machine application step 1 B Cells grown in a monolayer Cells grown in a monolayer up to 5 x 10 cells Cells grown in a monolayer can be detached from the culture flask by either trypsinization or using cell scraper To trypsinize cells Determine the number of cells Aspirate the medium and wash cells with PBS not provided Aspirate the PBS and add 0 10 0 25 trypsin After cells have detached from the dish or flask collect them in medium and transfer the appropriate number of cells to 5 x 10 cells to a 1 5 ml microcentrifuge tube not provided Centrifuge for 5 min at 300 x g Remove the supernatant completely and discard taking care not to disturb the cell pellet Continue with machine application step 1 Using a cell scraper Detach cells from the dish or flask Transfer the appropriate number of cells up to 5 x 10 cells to a 1 5 ml microcentrifuge tube and centrifuge for 5 min at 300 x g Remove the supernatant completely and discard taking care notto
22. ons www anatoliageneworks com Magnesia 16 Running Time 57 min sample volume 200 ul 66 min sample volume 400 ul Preparation before using 1 Add 1 0ml RNase Free Water to the Carrier RNA tube and mix by vortexing Store prepared Carrier RNA 1mg ml at 20 C 2 Add 1 1ml PK Storage Buffer to the Proteinase K tube and mix by vortexing Store prepared Proteinase K 10mg ml at 4 C For long term storage aliquot and store at 20 C Protocol 1 Pipet 10 for 200 ul sample or 20 ul for 400 ul sample Carrier RNA 1mg ml into the sample tube and add Proteinase K 20 ul for 200 ul sample or 40 ul for 400 ul sample into the 1 5 ml sample tubes provided 2 Add 200ylor400yl of serum into the sample tubes 3 Placing the 1 5 ml sample tubes into the MagCore T rack Run Code 202 program at Magnesia 16 Introduction of each Magnesia 16 Nucleic Acid Extraction kit Magnesia 16 Genomic DNA Tissue Kit For extracting Genomic DNA from a variety animal tissues paraffin embedded tissue swab and blood stain by using Magnesia 16 System of Anatolia Inc Cartridge Code 401 Cat NoMGT 01 MGT 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MGT 01 Contents Pre filled Cartrige Reagent Cat No MGT 02 Contents Pipet 36 pcs Pipet Tip Uo de MX 36 pcs Tip Holdev Sample Tube iscas s RE e 36pcs Sample Tube
23. rocess to apply the prepared sample and select 400ul sample volume Method 2 Centrifuge protocol 1 Take2 5mlvvhole blood sample and centrifuge at 3 000rpm 10mins 2 Use plastic drop to take white buffy coat layer in the middle of whole blood sample 3 Move the buffy coat into new microcentrifuge tube 4 Take 80 1001 buffy coat sample into Magnesia 16 sample tube and add Anatolia Lysis Buffer or PBS until 400ul then add 20yl proteinase 5 Follow Magnesia 16 process to apply the prepared sample and select 400ul sample volume Note We suggest to select 150 200ul elution buffer it can get better elution efficiency in both of these methods Normally the concentration is higher than 150ng ul under such elution volume Introduction of each Magnesia 16 Nucleic Acid Extraction kit v 20100615 Magnesia 16 Genomic DNA Large Volume Whole Blood Kit For purification of genomic DNA from human whole blood by using Magnesia 16 of Anatolia Inc Cartridge Code 104 Cat No MGB1200 Kit Contents Check that the following parts are included in addition to the main unit Cat No MGB1200 Contents Pre filled 77 Reagent ceat natio 96 pcs Pipet mu 100 pcs Tip Holder ae 100 pcs Sample Tube siaii 100pcs Elution Eppendorf Tu N 100pcs Proteinase K 11mq 8 PK Storage Butter 1 2510 oti 8pcs Storage and Stability 1 The kit shoul
24. roteinase Kand mix by vortex Store prepared Proteinase K 10mg ml at 20 C Protocol 1 Pipet Proteinase K 80 ul into the 1 5 ml sample tubes provided 2 Add 1200 ul whole blood into the sample tubes 3 Placing the 1 5 ml sample tubes into the Magnesia T rack Run Code 104 program at Magnesia 16 Introduction of each Magnesia 16 Cultured cells DNA Kit Magnesia 16 Cultured cells DNA Kit For Genomic DNA purification from cultured cells by using Magnesia 16 of Anatolia Inc Cartridge Code 110 Cat No MCC 01 MCC 02 Kit Contents Check that the following parts are included in addition to the main unit Cat No MCC 01 Contents Cat No MCC 02 Contents Pre filled Cartrige Reagent 3 PCS Pre filled 96 pcs PHD COTA 36 pcs Pipet TID 100 pcs e 36 pcs n 100 pcs SampieTubez 36 pcs Sample Iioc si 100 pcs Elution Epperidort T be ns 36 pcs Elution Eppendorf Tube 100 pcs Proteinase K 11mq IPCs Proteinase K 11mgq 2 6 PK Storage Buffer 1 2 9 2 1 PCS PK Storage Buffer 1 25ml eterni 2pcs Storage and Stability 1 The kit should be stored at room tempera
25. suitable for PCR or other enzymatic reactions Applications For general laboratory use Using magnetic particle technology to purified genomic DNA from fresh whole blood The purified genomic DNA can be directly used for downstream application like quantitative PCR restriction enzyme digestion southern bloting www anatoliageneworks com Magnesia 16 Running Time 44 min sample volume 200 ul 57 min sample volume 400 ul Preparation before using 1 Add 1 1mIPK Storage Buffer into the tube of Proteinase Kand mix by vortex Store prepared Proteinase K 10mg ml at 20 C Whole Blood Protocol 1 Take a new Sample Tube and add 20ul Proteinase K 10mg ml to per 200ul of equilibrated whole blood sample 40ul Proteinase Kto 400ul whole blood 2 Place the Sample Tube into hole 4 of T Rack Buffy Coat modify Protocol Anatolia Lysis Buffer 150mMNH C 10mM KHCO 0 1mM EDTA Method 1 Anatolia Lysis protocol 1 Take600 700ul whole blood into 2ml microcentrifuge tube Don t take more than 700ul whole blood sample it will cause the leakage situation during process Add 1ml Anatolia Lysis Buffer and mix the buffer and whole blood sample by upside down Shakethemixture 100rpm 5mins Centrifugethe mixture 13 000rpm 1 min Discard supernatant Repeat step 2 step 5 to wash the sample again Add 400u Anatolia Lysis Buffer and 20ul proteinase Kto resuspend the pellet HD KR W Follow Magnesia 16 p
26. tic machine Magnesia 16 The kit contains all required reagent and labware for automated purification using magnetic particle technology Reagents are supplied in prefilled cartridges which can be loaded into machine directly without extra work Easy select program code number 502 in Magnesia 16 and combine using Magnesia 16 Genomic DNA Bacterial Kit can perform high quality genomic DNA Applications For general laboratory use Using magnetic particle technology to purified genomic DNA from both Gram and Gram bacteria The purified genomic DNA can be directly used for downstream application like quantitative PCR restriction enzyme digestion southern bloting www anatoliageneworks com Magnesia 16 Running Time 44min sample volume 200 ul Preparation before using 1 Add 1 1ml PK Storage Buffer into the tube of Proteinase Kand mix by vortex Store prepared Proteinase K 10mg ml at 20 C 2 Immediately prepared 20mg ml Lysozyme solution with Lysozy me Reaction Buffer before use Protocol 1 Harvest cells maximum 2 x 10 cells in a microcentrifuge tube for 3min at 5000 x g 8000rpm Discard supernatant 2 Vortex the cell pellet then resuspend bacterial pellet in 200ul Lysozyme solution by vortexing or pipetting Incubate for at least 30min at 37 C During incubation vortex the tube every 5min 3 Add 4ul RNAse 50mg ml to sample mixture including any precipitate then to sample mixture and vortex to mix sample Incubate
27. ture 2 Proteinase K should be stored at 20 C when mixing with PK Storage Buffer Cartrige Contents lk elsi i iF T iZ es es S S T a a 84 Kas e S RA gt 5 m S 1S 88 88 A A m amp RR EIE 2131250515 06 3 ee Ge 3 8 4 S X 2 a DA o pd a a 8 8 S Description Magnesia 16 Cultured cells DNA Kit is designed to extract genomic DNA from up to 5x10 cultured cells via automatic machine Magnesia 16 The kit contains all required reagent and labware for automated purification using magnetic particle technology Reagents are supplied in prefilled cartridges which can be loaded into machine directly without extra work Easy select program code number 1 10 in Magnesia 16 and combine using Magnesia 16 Cultured cells DNA Kit can perform high quality genomic DNA Applications For general laboratory use Using magnetic particle technology to purified genomic DNA from 5x10 cultured cells The purified genomic DNA can be directly used for downstream application like quantitative PCR restriction enzyme digestion southern bloting m www anatoliageneworks com Magnesia 16 Running Time 44 min sample volume 200 ul up to 5 x 10 cells Preparation before using 1 Add 1 1mIPK Storage Buffer into the tube
28. uation during process Add 1ml Anatolia Lysis Buffer and mix the buffer and whole blood sample by upside down Shake the mixture 100rpm 5mins Centrifugethe mixture 13 000rpm 1 min Discard supernatant Repeat step 2 step 5 to wash the sample again Add 400ul Anatolia Lysis Buffer and 20ul proteinase Kto resuspend the pellet DH KR LNN Follow Magnesia 16 process to apply the prepared sample and select 400ul sample volume Method 2 Centrifuge protocol Take2 5ml whole blood sample and centrifuge at 3 000rpm 10mins Use plastic drop to take white buffy coat layer in the middle of whole blood sample Movethe buffy coat into new microcentrifuge tube Wow Take 80 100yul buffy coat sample into Magnesia 16 sample tube and add Anatolia Lysis Buffer or PBS until 400ul then add 20yl proteinase 5 Follow Magnesia 16 process to apply the prepared sample and select 400ul sample volume Note We suggest to select 150 200ul elution buffer it can get better elution efficiency in both of these methods Normally the concentration is higher than 150ng ul under such elution volume Introduction of each Magnesia 16 Nucleic Acid Extraction kit Magnesia 16 Genomic DNA Whole Blood Kit For purification of genomic DNA from human whole blood by using Magnesia 16 of Anatolia Inc Cartridge Code 102 Cat No MGB400 03 MGB 400 04 Kit Contents Check that the following parts are included in addition to the m
29. y mouth Wear protective disposable gloves laboratory coats and eye protection when handling samples and kit reagents Do not use sharp or pointed objects when working with the reagent cartridge in order to prevent damage of the sealing foil and loss of reagent Do not contaminate the reagents with bacteria virus or ribonuclease Use disposable pipettes and RNase free pipette tips only to remove aliquots from reagent bottles Use the general precautions described in the literature Wash hands thoroughly after handling samples and test reagents Waste Handling Discard unused reagents and waste in accordance with country federal state and local regulations www anatoliageneworks com How to use kit Install all necessary accessories and apply your specimen to Magnesia 16 WBL ITE CES PRESS START TO RUN Sma Press START v 1 2 3 gu 71819 mus After press Start button machine runs program of calibration initialize to move all axis to original factory positions v INPUT CARTRIDGE CODE 3 DIGITALS XXX Input Cartridge Code to protocol Cartridge Code is shown on your Reagent Cartridge and the cover of user manual Above CODE is for demostration purpose please refer tothe kit you purchasefor real workshop v INPUT CARTRIDGE CODE 3 DIGITALS 00 0 Enter NEXT PAGE
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