Class II, Type B2 (Total Exhaust) Biological Safety
Contents
1. E High negative pressure I Negative pressure J Positive pressure The Esco triple wall design creates a Dynamic Chamber plenum which surrounds contaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities Comfortable Ergonomic Design The LB2 cabinet is engineered for comfort utility value and safety e The 10 angled viewing window and narrow profile front grille improves reach into the work area e The instant start 5000k fluorescent lamp operates on an electronic ballast to reduce heat improve comfort and conserve energy e The lamp delivers uniform lighting to the work surface for greater comfort reduced glare and improved productivity see Technical Specifications e The front armrest is raised above the workzone to improve comfort and Air U 2 NSF ANSI 49 USA LB2 4B2 LB2 6B2 SFDA YY 0569 China Air Quality ISO 14644 1 Class 3 Worldwide JIS B9920 Class 3 Japan JIS BS5295 Class 3 Japan US Fed Std 209E Class 1 USA to ensure that the operator s arms do not block the forward airflow perforations e The optional adjustable support stand provides work surface height control e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of2. P Labculture Labculture o H SFDA O Labculture Class Il Type B2 Total Exhaust Biological Safety Cabinet Model LB2 4B_ Class Il Type B2 Total Exhaust Biological Safety Cabinets The Safety Solution for Life Science Laboratories Ioculture i r Main Features gt L Unique Esco Dynamic Chamber plenum Negative pressure plenum surrounds supply positive pressure plenum no fabric bags are used Long life supply ULPA filter per IEST RP CC001 3 with gt 99 999 efficiency for particle size between 0 1 to 0 3 microns Angled supply filter matches cabinet profile to achieve best downflow uniformity li Esco Sentinel microprocessor Frameless shatterproof sash is easier to clean Ergonomically angled front improves reach and comfort Actual work access height is 45 mm 1 8 higher than tested sash opening to provide additional work space Removable one piece work surface simplifies cleaning a Raised airflow grille maintains safety by preventing L aa ae ol a as Optional UV lamp is located behind control panel away from line of sight Does not apply to 1 8 m 6 models F Esco ISS LiDE antimicrobial coating on all painted surfaces minimizes contamination Enhanced side capture zones optimize containment Work area on Esco 0 9 meter 3 cabinet i iS SFDA Qe Esco Labculture has passed more performance tests i
3. filters that have a typical efficiency of gt 99 99 for 0 3 micron particles e HEPA exhaust filter per IEST RP CC001 3 is tested to a typical efficiency of gt 99 99 for 0 3 micron particles Mini pleat Separatorless Filter left vs Conventional Aluminium Separator Filter right Esco cabinets use Swedish Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame for structural stability and elimination of swelling common to conventional wood frames Esco ULPA Filter Efficiency Typical Penetration Modern separator less mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction The filter frame and media is constructed in accordance with EN1822 requirements for fire retardant properties The supply ULPA filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection The exhaust HEPA filter traps biohazard particles acquired from the work surface before air is exhausted via the ducting system to the external environment The Model LB2 is provided with an integral exhaust collar for connecting the cabinet exhaust to an airtight ducting system
4. 3B_ LB2 4B _ LB2 5B_ LB2 6B_ 1 8 meters 6 a ahoue pace stand 1115x815x1568mm 1420x815x1568mm 1725x815x1568mm 2030x815 x 1568 mm 43 9 x 32 0 x 61 7 55 9 x 32 0 x 61 7 67 9 x 32 0 x 61 7 79 9 x 32 0 x 61 7 With Optional Base Stand 711mm 28 1115x815x227 9mm 1420x815 x2279 mm 1725x815x22 9mm 2030x 815x2279 mm type 43 9 x 32 0 x 89 7 55 9 x 32 0 x 89 7 67 9 x 32 0 x 89 7 79 9 x 32 0 x 89 7 955 x 623 x 715 mm 1260 x 623 x 715mm 1565 x 623 x 715mm 1870 x 623 x 715mm 37 6 x 24 5 X25 49 6 x 24 5 x 28 1 61 6 x 24 5 x 28 1 73 6 x 24 5 x 28 1 0 44 m 4 7 sq ft 0 59 m 6 4 sq ft 0 74 m 8 0 sq ft 0 89 m 9 6 sq ft 203 mm 8 0 203 mm 8 0 203 mm 8 0 203 mm 8 0 248 mm 9 8 248 mm 9 8 248 mm 9 8 248 mm 9 8 Inflow 0 53 m s 105 fom Downflow 0 35 m s 70 fpm Inflow 376 m h 223 cfm 492 m h 292 cfm 608 m h 361 cfm 724 m h 429 cfm Downflow 709 m h 424 cfm 928 m h 555 cfm 1147 m h 686 cfm 1367 m h 817 cfm Certification Exhaust Inflow Downflow 1085 m h 647 cfm 1420 m h 847 cfm 1755 m h 1047 cfm 2091 m h 1247 cfm Concurrent Balance Value Exhaust Volume at corresponding Static Pressure 1215 m h 724 cfm 1590 m h 948 cfm 1966 m h 1172 cfm 2342 m h 1396 cfm Note Use this for HVAC sizing Minimum exhaust static pressure for clean exhaust filter 499 P
5. OQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 711 mm 28 or 864 mm 34 With leveling feet 38 1 mm 1 5 With casters e Telescoping height stand for leveling feet nominal range 660 mm or 960 mm 26 or 37 8 e Telescoping height stand for casters nominal range 660 mm or 880 mm 26 or 34 6 Adjustable in 25 4 mm 1 increments e Cradle stand electrical hydraulic infinitely adjustable with casters Elevates to seating or standing work surface height When lowered permits movement through standard doorway Note Increases exterior dimensions All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating substrate and cannot wash out or diminish by repeated cleaning Performance results are available upon request Contact Esco or your Esco Sales Representative for details Model LB2 Biological Safety Cabinet Technical Specifications 271 mm 10 7 LB2 3 430 mm 16 9 LB2 4 666 mm 26 2 LB2 5 820 mm 32 3 LB2 6 50 mm 2 0 J 955 mm 1260 mm 15
6. in RFI and electrical noise filters eliminate interference with adjacent instrumentation e An integral blower hour meter tracks operating life and aids in predictive maintenance planning e To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Labculture Esco cabinets use German made ebm papst permanently lubricated centrifugal motor blowers with external rotor designs ntegrated blades norrow the profile and eliminate the need for a motor shaft Motors are selected for energy efficiency compact design and flat profile The completely integrated assembly optimizes motor cooling All rotating parts are unitized and balanced for smooth quiet vibration free operation Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture
7. kg 615 Ibs 319 kg 703 Ibs 1210 x 940 x 1930 mm 47 6 x 37 0 x 75 9 317 kg 699 lbs 381 kg 840 Ibs 1520 x 940 x 1930 mm 59 8 x 37 0 x 75 9 359 kg 791 Ibs 393 kg 866 Ibs 1800 x 970 x 2020 mm 70 8 x 38 1 x 79 5 438 kg 966 Ibs 491 kg 1082 Ibs 2100 x 970 x 2020 mm 82 6 x 38 1 x 79 5 2 19 m 77 3 cu ft 2 76 m 97 4 cu ft 3 53 m 124 6 cu ft 4 11 m 145 1 cu ft This Concurrent Balance Value CBV Exhaust per Pitot Duct Traverse and Static Pressure must be used when sizing the HVAC exhaust amp supply ae This minimum exhaust static pressure for clean exhaust filter should not be used for exhaust fan sizing and it is listed here for comparative purpose only kkk KKKK Noise reading at open field condition anechoic chamber Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand Comprehensive Performance Testing At Esco Labculture Inflow downflow velocity Light noise and vibration Airflow pattern visualization Flectrical safety to EC61010 1 Additional KI Discus containment and microbiological testing is performed on statistical sampling basis Every Labculture LB2 model manufactured by Esco is individually tested documented by serial number and validated with the following test methods PAO aerosol challenge for filter integrity Biological Safety Ca
8. number shall be provided with the cabinet and maintained in the manufacturers records Test data verifying all performance criteria shall be available upon request to include a inflow velocity through direct inflow measurement method b downflow velocity and uniformity c filter leak scan with aerosol challenge for both filters d light noise vibration e and electrical safety Filtration System 5 The cabinet shall have one supply downflow filter and one exhaust filter Supply filter shall be ULPA type per IEST RP CC001 3 and meet EN1822 H14 requirements Exhaust filter shall be HEPA type per IEST RP CC001 3 and meet EN 1822 H13 requirements Labculture The filters shall be within an aluminum frame with mini pleat design without aluminum separators no wood or fiberboard shall be used in the filter assembly Typical ULPA filter efficiency shall be gt 99 9997 at MPPS and 99 999 for 0 1 to 0 3 microns Typical HEPA Filter shall be gt 99 99 at 0 3 microns An integral filter guard shall be affixed to prevent damage to the filter media 9 The filters shall be a individually scan tested by 10 11 the manufacturer b individually scan tested after assembly and b easily accessible for scan testing in situ by means of a dedicated upstream sampling port accessible from within the cabinet The supply filter shall be angled and oriented to the 10 cabinet front angle to maximize downflow un
9. 65 mm 1870 mm 250 mm 9 8 250 mm 9 8 1568 mm 61 7 37 6 49 6 61 6 73 6 1115 mm 1420 mm 1725 mm 2030 mm E 43 9 56 0 67 9 80 0 7 Exhaust HEPA filter 6 2 Blower 3 Downflow ULPA filter angled 4 UV light Retrofit Kit provision 5 IV Bar Retrofit Kit provision 7 0 9 meter 8 Fluorescent lamp Flectrical outlet Retrofit Kit provision outlet in workzone 1 2 1 5 and 1 8 meter 4 5 amp 6 models two single outlets in workzone Electrical Electronics panel 3 model one single 2 on each side control system 11 Stainless steel armrest 9 Plugged service fixture provisions 10 Stainless steel single piece work tray 12 Drain valve Retrofit Kit provision 13 Esco Sentinel microprocessor 815 mm 32 1 14 Safety glass sliding sash window 15 Single piece stainless steel back wall and side walls 16 Removable side panel for plumbing access 17 Integral exhaust collar Accessories and Options Cont Electrical Outlets and Utility Fixtures e Electrical outlet ground fault North America e Electrical outlet Europe Worldwide e Petcock air gas vacuum North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories e Germicidal UV lamp Controlled by automatic UV lamp timer through Sentinel micro processor control p
10. NB Ductworks are not provided with standard product Front Sash Assembly Integrated sash proximity contacts sense sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned The magnetic switch eliminates the chance of mechanical wear and tear typical of a mechanical switch The back of the sash can be easily cleaned by removing the sash track cover and swing up the sash glass The sash is counterbalanced for smooth and light sash moving operation The counterbalance locking mechanism is inherently safe it locks the counter balance in place if either of the 2 cables is detached The sash cable and cable clip have rated strength of more than 6x the weight of the sash window of the largest Labculture Class II cabinet 1 8 meter 6 ft model e The safety glass maintains containment if the sash is accidentally broken during cabinet operation Esco cabinets use supply ULPA filters per IEST RP CC001 3 instead of conventional HEPA filters commonly found in biological safety cabinets While HEPA filters offer 99 99 typical efficiency at 0 3 micron level ULPA filters 0 0 05 0 10 0 15 0 20 0 25 0 30 Particle Size um 0 40 provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 micron level ESO WORLD CLASS WORLDWIDE 0 50 ULPA filtered air C Unfiltered potentially
11. a 2 0 in H O 479 Pa 1 9 in H 0 434 Pa 1 7 in H O 505 Pa 2 0 in H 0 Static Pressure with additional 174 Pa 0 7 in H O required by NSF ANSI 49 2008 Note Use this for HVAC sizing 673 Pa 2 7 in H O 653 Pa 2 6 in H 0 608 Pa 2 4 in H 0 679 Pa 2 7 in H O Supply ULPA Filter Typical Efficiency gt 99 999 for particle size between 0 1 to 0 3 microns Exhaust HEPA Filter Typical Efficiency Sound Emission Fluorescent Lamp Intensity At Zero Ambient Cabinet Construction Electrical Net Weight Shipping Weight Shipping Dimensions Maximum W x D x H Shipping Volume Maximum gt 99 99 at 0 3 microns gt 116 foot candles gt 130 foot candles gt 111 foot candles NSF ANSI 49 lt 58 dBA lt 59 dBA lt 60 dBA lt 61 dBA EN 12469 lt 55 dBA lt 56 dBA lt 57 dBA lt 58 dBA gt 1250 Lux gt 1400 Lux gt 1200 Lux gt 1200 Lux gt 111 foot candles LB2 4B2 LB2 6B2 1 5 mm 0 06 16 gauge electrogalvanized steel with Isocide white oven baked epoxy polyester powder coating All other variants 1 2 mm 0 05 18 gauge electrogalvanized steel with Isocide white oven baked epoxy polyester powder coating 220 240V AC 50Hz 10 LB2 3B1 LB2 4B 1 LB2 5B1 LB2 6B1 110 120V AC 60Hz 1 LB2 3B2 LB2 4B2 LB2 5B2 LB2 6B2 220 240V AC 60Hz 10 LB2 3B3 LB2 4B3 LB2 5B3 LB2 6B3 279
12. anel Emission of 253 7 nanometers for most efficient decontamination Lamp is positioned away from operator line of sight for safety and proper exposure to interior surfaces Note UV lamp intensity reduces over time and its effectiveness is subject to factors such as relative humidity in the cabinet ambient air temperature and microbial species in the work zone e PVC armrest Chemically treated improves operator comfort easy to clean 711 mm 28 standard size e Ergonomic lab chair Laboratory grade construction meets Class 100 cleanliness alcohol resistant PVC materials Adjustable 395 490 mm 15 6 19 3 e Ergonomic foot rest Angled helps maintain proper posture Adjustable height Anti skid coating chemical resistant finish IV bar with hooks Stainless steel construction Available for all standard Esco cabinets Microscope viewing device Mounting and viewing pouch integrated into sash Factory installed specify when ordering Anti blowback valve Prevents flow from the facility HVAC system into the Esco product Air tight damper Seals the Esco product from the facility HVAC system during decontamination ESCO WORLD CLASS WORLDWIDE Nominal Size Dimension External WxDxH Internal Dimensions W x D x H Usable Work Area Tested Opening Working Opening Average Airflow Velocity Airflow Volume General Specifications LB2
13. atic shut off managed by the microprocessor controller and shall be interlocked with the blower motor and fluorescent lights for safety The cabinet shall be designed with a 10 angled front to optimize user comfort reduce glare and maximize reach into the work area The front grille shall be raised to prevent airflow blockage and improve comfort Penetrations for petcocks and service fittings shall be provided penetrations shall be offset to improve user access The cabinet shall accommodate an optional mounting stand for fixed height Control and Alarm System 33 34 35 36 37 38 39 40 41 42 All cabinet functions shall be managed by a programmable microprocessor control system capable of software updates via Internet downloads The microprocessor controller shall be mounted on the main control panel facing down toward the user The controller shall include soft touch keypad controls and backlit LCD displays to permit operation of the blower motor light UV lamp electrical outlet s and menu The controller shall be user programmable in situ to enable or disable functions such as PIN personal identification number access restriction cabinet start up protocol airflow alarm and other microprocessor controlled operations outlined in the user manual When programmed ON the start up protocol shall perform an automatic pre purge and post purge cycle to ensure proper cabinet operat
14. binets Class Il Type B2 Total Exhaust Biological Safety Cabinets Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and international criteria Testing in our microbiology laboratory is conducted according to NSF ANSI 49 EN 12469 and JIS K3800 An NSF accredited biohazard cabinet field certifier is available in house full time to supervise all tests Harmless Bacillus atrophaeus formerly Bacillus Subtilis bacteria is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Units CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet workzone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B atrophaeus spores is placed inside the workzone 10 cm 4 inches behind the front opening sash e Target slit air samplers and impingers are placed outside the workzone to capture B atrophaeus spores that may escape then the sample is incubated e Acceptance The number of B atrophaeus CFU recovered from th
15. contaminated air L Room air Inflow air Blower Efficiency The Labculture blower system is de signed for high performance operation maximum energy efficiency and minimal maintenance e The blower motor can maintain airflow velocity within 10 of original setpoint for a 50 increase in pressure loss over clean filters without manual speed control adjustment e The permanently lubricated direct drive external rotor motor blower reduces operating costs e The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor bearing life ee n Ss Cahinet Filtratior Capinet Frilttratior Side capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the front grille to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Inflow air travels through a return path toward the common air plenum blower plenum at the top of the cabinet Ambient air is taken in through a pre filter at the top of the cabinet and passes through the downflow ULPA filter entering the work zone as laminar flow The uniform non turbulent air stream protects against cross contamination within and throughout the work area The uniform non turbulent air stream protects against cross contamination within and throughout the work area e Built
16. cts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities The cabinet work zone has no welded joints to collect contaminants or rust All stainless steel work surfaces are accessible for cleaning Tray components lift and remove to provide easy access and encourage surface decontamination e There are no screws in on the front or sides to trap contaminants or complicate cleaning e Optional service fittings are offset for easier access e External plumbing is concealed behind trim panels to maintain aesthetics Robust construction and enhanced safety features qualify the cabinet for the most demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped e A recessed central area and stainless External surfaces are coated with steel drain pan channels spills and Esco Isocide antimicrobial coating to prevent liquids from entering the lower protect against surface contamination filtration and blower systems and inhibit bacterial growth Isocide eliminates 99 9 of surface bacteria within 24 hours of exposure e The drain pan is flush with the side walls to eliminate concealed or hard to clean spaces e The interior work area is formed from a single piece of stainless steel with large radius corners to simplify cleaning Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset for easier reach Stan
17. dard cabinets include two fixture provisions on each sidewall one provision on each sidewall for 0 9 meter 3 ft cabinet Electrical outlets are mounted below service fixtures to minimize obstructions Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access All key components with the exception of the blower motor assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing potentially hazardous areas or components such as microbiological contamination or electrical shock are color coded red to warn service technician The Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when required Work area containment is maintained even when removable components are lifted out for cleaning The one piece stainless steel work tray edges are radiused and easy to clean without crevices or joints The lower drain trough is a single piece fabrication with wide open angles and a channel to direct spills to the drain The closed sidewall contains no perforations air return slots or other hidden areas where contaminants can accumulate ESCO WORLD CLASS WORLDWIDE Biosafety Cabinets Standards Compliance Dynamic Chamber Plenum Design
18. e agar plates shall not exceed 10 CFU per test Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet workzone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B atrophaeus is placed at 10 cm 4 inches in front of sash window e Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus atrophaeus CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the workzone e A nebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of the workzone sidewalls e Target agar plates are placed 36 cm 14 inches away from the same side wall as the nebulizer e Acceptance The number of Bacillus atrophaeus CFU recovered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontaminatable cabinets tested with both BIOQUELL and STERIS patented processes HPV is asafer and more efficient alternative to conventional decontamination using formaldehyde CH 0 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has punge
19. e Ultralow Freezers cco WORLD CLASS WORLDWIDE Esco Technologies Inc e 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040 USA Toll Free USA and Canada 877 479 3726 Tel 215 441 9661 Fax 215 441 9660 us escoglobal com usa escoglobal com Esco Micro Pte Ltd e 21 Changi South Street 1 e Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 mail escoglobal com www escoglobal com Esco Global Offices Breukelen The Netherlands Kuala Lumpur Malaysia Manama Bahrain Marietta OH USA Mumbai India Philadelphia PA USA Salisbury UK Shanghai China Singapore escoglobal com mm Rieka iISScibE Esco Micro Pte Ltd Cert No 651333 2010 Esco Micro Pte Ltd Specifications Subject to Change OW8480F TE ES1450_V2_3K_06 10 PT Esco Bintan Indonesia Cert No 651333 1
20. iformity over the work surface A removable perforated metal diffuser shall be installed below the supply filter to optimize airflow uniformity and to protect from damage Blower System 12 13 14 15 16 The cabinet shall have a direct drive permanently lubricated centrifugal blower motor dynamically balanced in two planes compliant to ISO 2710 for low noise low vibration and long filter life The blower motor shall have an external rotor design and include an automatic thermal cut out to disable the motor in case of overheating The blower motor shall have an automatic speed control to compensate for filter loading The blower motor system shall be enclosed within a dynamic chamber shaped steel plenum and integrated with the removable supply filter assembly to simplify filter changing The cabinet shall be equipped with an integral exhaust collar for connection to the laboratory ducting system Cabinet Design Construction Cleaning 17 18 19 20 21 22 The cabinet shall be of triple wall design whereby all positive pressure plenums capable of handling contaminated air shall be surrounded by negative pressure No positive pressure areas shall be accessible external to the cabinet The third wall shall conceal utilities The cabinet shall maintain containment performance even when removable work area components are removed for cleaning The work tray shall be one piece removable s
21. ion The controller shall include a blower motor hours meter to display aggregate motor running time to assist in predictive maintenance Audible and visual alarms shall be provided for unsafe conditions such as improper airflow or sash position Airflow shall be monitored by a temperature compensating thermistor based true air velocity sensor mounted in the cabinet The airflow display and alarm system shall be individually calibrated before shipment The main control panel shall exhibit continuous display of air velocity and a 24 hour clock display Certification Service and Decontamination 43 The cabinet shall be approved for both hydrogen peroxide vapor HPV and formaldehyde decontamination protocol 44 All panels leading to potentially contaminated and 45 46 or hazardous areas shall be color coded red All components with the exception of blower motor and ULPA filters shall be located outside of contaminated air spaces to facilitate servicing without the need to decontaminate the cabinet All exterior surfaces shall be painted with a permanent antimicrobial inhibitor coating to minimize contamination Installation for Labculture Class II Type B2 Total Exhaust Biological Safety Cabinet Supply Air Make Up for Downflow and Inflow Air Note Cabinet shown with optional support stand optional air tight damper optional anti blowback valve and optional exhaust blower Exhaust d
22. n be activated or deactivated depending on user preference and nature of the work Touchpad data entry buttons permit control settings and access to diagnostics default Programmable automatic UV light timer simplifies operation enhances A graphical interface indicates cabinet performance settings and hierarchical menus contamination control extends UV lamp life and saves energy Color coded indicator lamps display green for primary function fan operation blue for secondary function fluorescent lights and electrical outlet and orange for caution UV lamp ON Sentinel Microprocessor Control System Programmable Consult your Esco Operating Manual or contact your Sales Representative for information on user preference programming capabilities built into the Sentinel microprocessor platform Cabinet Construction Digital read out with alpha numeric display indicates all input status and alarm functions All functions can be user activated through touch pad programming access see Operations Manual Read all satety related instructions balore use er a ii i a tr Ta bal as e ap P pL aieit Sahay BPrvecion Stard SO 9001 Quality Certified EC 10i0 i 1 mioAare Grulactuning Enveronnmsni cortity this cabinet at least annually ESCO When programmed ON e the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restri
23. n more languages for more certifications throughout more countries than any other biological safety cabinet in the world Each cabinet is Kl Discus tested for performance integrity Available in 0 9 1 2 1 5 and 1 8 meter models 3 4 5 and 6 Shown with optional telescoping stand Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets b Labculture Operator Product and Environmental Protection The Esco Labculture Class Il Type B2 Biological Safety Cabinet provides operator product and environmental protection against Biosafety Levels 1 2 and 3 This cabinet can be used for handling Biohazard Level 4 provided that the operator wears positive pressure sult Containment and Protection e The total exhaust design of the LB2 makes the cabinet suitable for microbiological work involving trace amounts of toxic chemicals The non recirculating design guarantees safety in cases where exhaust HEPA filtration fails to ensure containment Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination The front grille has proportionally larger perforation on the extreme left and right side Combined with the side air gap between the work tray and the side wall the stronger side capture zones increase protection in this critical area where contaminants tend to bleed out in co
24. nt smell e HPV biological efficacy is independent of environmental variables whereas formaldehyde efficacy is dependent on such variables e lf there is a gap on the cabinet sealing escaping HPV to the lab will decompose to become oxygen and water Escaping The Performance Envelope Concept 0 65 formaldehyde however is harmful to people in the lab Therefore HPV decontamination can be performed while people still working inside the lab while formaldehyde decontamination must be performed with no one present in the lab The HPV method improves safety productivity and reduces the time to seal the cabinet HPV has a better penetration capacity resulting in a full decontamination of the cabinet The formaldehyde method is known to result in incomplete decontamination HPV is more effective and rapid against biological organisms compared to formaldehyde HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to complete a formaldehyde decontamination process HPV decontamination effectiveness is independent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 For information on the BIOQUELL and STERIS HPV methodologies contact Esco or your Sales Representative and ask for our HPV Decontamination Whitepapers Esco cabinets are designed to operate within a High Inflow High Downflo
25. nventional cabinet The inflow grille has a convex shape to maintain operator protection by discouraging users from placing objects on it Raised armrest prevents the likelihood of inflow grille blocking by operator s arms Auto purge holes located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window Per NSF ANSI 49 requirement these side perforations do not extend completely to the work surface to prevent accidental liquid spills in the work zone from entering the side air column e The ULPA downflow supply filter is tilted proportional to the cabinet front angle to direct more air forward to the front air grille e The inflow velocity downflow velocity and air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front aperture this curtain maintains personal and product protection even in the unlikely event of a severe inflow or downflow imbalance that would compromise protection in a conventional cabinet Integrated Filtration System A combination of a supply ULPA filter and an exhaust HEPA filter give the Labculture cabinet a fully integrated performance envelope for product operator and environmental protection e ULPA supply filter per IEST RP CC001 3 is tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron particles providing better filtration capability than conventional HEPA
26. small equipment See Specifications e The sliding window can be fully opened to insert and remove larger instrumenta tion and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually factory tested for electrical safety e Documentation specific to each cabinet serial number is maintained on file e UL Listed for USA and Canada e Certified to the US standard NSF ANSI 49 for Class Il biohazard cabinetry refer to Standard Compliance table at the top of this page for the full list e Contact Esco or your Sales Representative for site preparation information see Electrical Specifications Warranty The Labculture cabinet is warranted for 3 years excluding consumable parts and accessories iE Antimicrobial Powder Coating Bacterial Population 10 folds 0 4 hours 8 hours Labculture 12 hours 20 hours 24 hours 16 hours Contact Time in Hours Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Filtration EN 1822 H14 amp H13 Europe IEST RP CC001 3 USA IEST RP CC007 USA IEST RP CC034 1 USA Electrical Safety UL 61010 1 USA CAN CSA C22 2 No 61010 1 EN 61010 1 Europe IEC 61010 1 Worldwide e Each cabinet is shipped with a comprehensive user s manual complete with a report documenting all test procedures e Additional IQ
27. tainless steel with radius corners without crevices or joints The cabinet shall have a stainless steel one piece fabricated drain trough with open angles to channel spills to a common drain The closed sidewall shall be sealed without perforations return air slots or concealed areas which can contain contaminants The cabinet shall be free of sharp edges non functional protrusions bolts screws or hardware and all metal edges shall be deburred Ergonomics and Convenience 23 24 25 The front sash shall be frameless to maximize visibility and accessible for cleaning front and back Sash glass shall be safety glass The sash counterbalance shall be suspended on two high strength cables and the sash shall lock into position in the event one cable becomes detached Magnetic not mechanical proximity sensors shall work in conjunction with the control system to indicate proper sash position for containment Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets 26 27 28 29 30 31 32 Fluorescent lamps shall be mounted behind the control panel module out of the work zone Electronic ballasts shall be used to eliminate flicker extend lamp life and reduce heat output The UV lamp if installed shall be mounted behind the control panel and away from the user s line of site for protection The UV lamp shall operate via an automatic timer with autom
28. tection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection and is useful for validating the actual containment performance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus Esco Labculture LB2 models are factory tested on a sampling basis using the KI Discus method for operator safety Purchase Specifications LB2 Series Class Il Type B2 Total Exhaust Biological Safety Cabinet General Performance and Certifications 1 The biological safety cabinet shall comply with one or more of the following international standards and the manufacturer shall provide a certified copy of containment and performance tests equivalent to or greater than specified in the following independent international standards for biological safety electrical and other functional characteristics Class Il Type B2 per NSF ANSI 49 USA 2 The cabinet shall protect a the operator and laboratory environment from particulates generated within the work zone b the product and process within the work zone from airborne contamination from ambient air c and the product and process within the work zone from cross contamination 3 Each cabinet shall be listed by Underwriters Laboratories UL cUL or CE for electrical safety 4 Original documentation specific to each cabinet serial
29. twork on site ESEO WORLD CLASS WORLDWIDE Biological Safety Products Cleanroom Products Containment Pharma Products Ductless Fume Hoods Carbon Filtration General Purpose Scientific Equipment Industrial Lab Equipment In Vitro Fertilization Products PCR Products Pharmacy Products Lab Animal Research Products Lab Thermostatics Products Lab Ventilation Chemical Fume Products Lab Furniture Powder Handling Products Since 1978 Esco has emerged as a leader in the development of controlled environment laboratory and cleanroom equipment solutions Products sold in more than 100 countries include biological safety cabinets cleanroom products compounding pharmacy equipment containment pharma products ductless fume hoods in vitro fertilization workstations lab animal research products laboratory fume hoods laboratory ovens and incubators laminar flow clean benches and PCR products and instrumentation With the most extensive product line in the industry Esco has passed more tests in more languages for more certifications throughout more countries than any biosafety cabinet manufacturer in the world Esco remains dedicated to delivering innovative solutions for the clinical life science research and industrial laboratory community www escoglobal com Biological Safety Cabinets and Laminar Flow e Laboratory Fume Hoods e Laboratory Ovens Laboratory Incubators e PCR Thermal Cyclers e Microplate Shaker Incubators
30. uctwork is not provided by Esco Check List 1 Ensure a Class II Type B2 cabinet is required for the application 2 Provide an exhaust system ductwork and blower capable of delivering the required airflow volume and static pressure at the connection point note Required Static Pressure listed on page 8 is for the cabinet only Exhaust Blower at Terminal End Preferably Connected to Emergency Power Pitot Tube Duct Traverse Penetration Drill Penetrations in Ductwork Anti Blowback Valve Optional Air Tight Damper Required iat ai f Enables static pressure to y Dini ev oe be adjusted as filter loads to maintain airflow volume Enables the cabinet to be sealed off during decontamination EE al vvvv 11 isoce Labculture 7 Observe Esco recommendations on suitable cabinet location 8 Certify cabinet after installation before use 4 Ensure the lab is supplied with sufficient makeup air remember to add the pressure loss for the specified airflow volume in the ductwork 3 A dedicated exhaust system one exhaust blower to one cabinet Is strongly recommended It is also recommended the exhaust system be operated continuously 5 Provide an air tight damper mandatory and anti blowback valve optional 6 Provide provisions for pitot tube duct traverse These can be drilled in the duc
31. w 0 61 0 43 m s performance envelope to maintain protection High Inflow for personnel product and the environment Low Downflow 0 60 0 58 0 30 Airflow parameters used to frame the performance envelope include both inflow velocity and downflow velocity Nominal Inflow Nominal Downflow 0 53 0 35 The graph illustrates the boundaries of the performance envelope as well as the nominal performance point at which tests are conducted The range between high and low Inflow and high and low downflow together with the fluid dynamics achieved through sophisticated cabinet design proportionally size capture slots and uniform laminar airflow combine to deliver a complete containment and safety solution expected of a professional biological safety cabinet 0 50 Inflow Velocity Low Inflow High Downflow 0 48 0 40 Low Inflow 0 45 nflow 0 48 0 30 0 40 For details on the Esco performance envelope 0 590 contact Esco or your Esco Sales Representative m s 0 25 0 30 0 35 0 40 0 45 Downflow velocity ESEO WORLD CLASS WORLDWIDE 10 KI Discus Containment Test According to EN 12469 Potassium lodide Esco is currently one of the few companies in the world equipped to perform the KI Discus test for our customers The KI Discus test is defined in the European Standard for microbiological safety cabinets EN 12469 as a test method for validating the operator personnel pro
32. zones at a higher velocity small blue arrows A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone The downflow combined with the inflow air enters the common air plenum All air in the common plenum is HEPA filtered and exhausted via a dedicated ducting system to the external environment The control panel is located on the center of the cabinet and angled down for easy access by the operator Continuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel e An integrated temperature compen sated true airflow velocity sensor provides an accurate airflow reading despite room temperature fluctuation All electronic parts are contained inside a plug and play module that permits easy exchange if required e Microprocessor software updates are available from Esco for download via the Internet Sentinel functions are factory set to default to ON or OFF depending on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access e Automatic start up sequence will prepare the cabinet for normal operation and advise when safe conditions are established e An administrator controlled PIN Personal Identification Number can be set to restrict access to main menu e The airflow alarm ca
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