UltraGAPS™ Coated Slides Instruction Manual with Protocols
Contents
1. AND HYBRIDIZATION OF DNA MICROARRAYS General Considerations Composition of spotting solution The chemical and physical properties of the spotting solution greatly influence DNA retention spot morphology and hybridization efficiency Corning recommends the use of the Pronto Universal Spotting Solution Cat No 40027 because of its low evapo ration rate and nuclease inhibiting properties Solvent evap oration causes the concentration of DNA and other non volatile components of the spotting solution to rise leading to time dependent changes in spot quality suboptimal array uniformity and the eventual loss of the spotting solution The most commonly used spotting solution in order of decreasing physical stability are Pronto Universal Spotting Solution 50 DMSO 3 x SSC supplemented with 1 5 M betaine 150 mM sodium phosphate and 3 x SSC These solutions have successfully been used to fabricate DNA arrays on UltraGAPS slides DNA dissolved in DMSO containing media may aggregate if solvent concentration exceeds 70 which happens upon prolonged use of the source plates and freeze thaw cycling Aqueous SSC and sodium phosphate containing media have a tendency to salt out which may cause quill pins to clog and require recon stitution after each print run which leads to noticeable variability in DNA concentration among source wells Preparation of probe DNA Double stranded DNA for spotting on microarrays is generally obtai2. between washes as this will result in high backgrounds Mul tiple containers are needed to perform the washes in the most efficient manner Have all containers and the volumes of wash ing solutions ready before starting the procedure Note that steps 2 and 3 both require solutions prewarmed to 42 C 1 Disassemble the hybridization chambers 2 Immerse arrays in 2 x SSC 0 1 SDS at 42 C until the coverslip moves freely away from the slide 3 Transfer arrays to 2 x SSC 0 1 SDS at 42 C for 5 minutes 4 Transfer arrays to 0 1 x SSC 0 1 SDS at room tempera ture for 5 minutes 5 Repeat step 4 6 Transfer arrays to 0 1 x SSC at room temperature for 1 minute 7 Repeat Step 6 four times 8 Rinse arrays in 0 01 x SSC for 10 seconds 9 Dry arrays by blowing clean compressed nitrogen or by Corning Microarray Products centrifugation at 1 600 x g for 2 minutes Cat No Product Description Qty Pk Qty Cs 10 Store arrays in the Corning 25 Slide Holder Cat No 40015 UltraGAPS Coated Slides with Bar Code 5 25 40081 Protect arrays from overexposure to light until ready to scan 40016 UltraGAPS Coated Slides witout Bar Code 5 25 Note Arrays spotted on UltraGAPS slides can be hybridized 40017 UltraGAPS Coated Slides vath Bar Code Bulk Pack 25 25 at temperatures up to 65 C The use of hybridization tempera 40018 UltraGAPS Coated Slides without B
3. concentrations to use as a starting point for further optimization are 0 10 mg mL when spotting dsDNA e g PCR products genomic DNA and 0 50 mg mL when spotting oligonucleotides Printing conditions and pin quality Follow the instructions provided by the manufacturer of arraying equipment and printing pins Printing should be performed under con trolled environmental conditions where temperature and relative humidity can be maintained at about 25 C and 45 respectively Pins should be selected according to their diam eter and loading capacity depending on the desired array density and the number of arrays to be printed For example quill pins of 50 microns in diameter can produce arrays con taining over 50 000 features when used to spot DNA dis solved in highly hygroscopic spotting solution such as 50 DMSO and Pronto Universal Spotting Solution Pin contact time and the force with which the pin strikes the slide also affect spot size and morphology Pins must be individually qualified before use Pins that are broken or otherwise do not conform to specifications must be replaced Printing and pin washing steps should be optimized during a test run in preparation for array fabrication Immobilization procedures Binding of DNA to the GAPS coated surface is enhanced by UV cross linking and or bak ing These procedures work equally well for DNA molecules longer than 300 bp Smaller DNA molecules and oligonucleo tides are best
4. evaluate the performance of a particular spotting medium under conditions that are as close to working conditions as possible before committing large sets of probes to the formulation Thorough and properly controlled print tests must be done in order to ensure that the desired spot density and array uniformity is achievable Once probe DNA is dissolved in a spotting medium it is very difficult to recover it for reconstitution in a different solvent 1 Prepare source plates sterile nuclease free 384 well storage plates are recommended Cat Nos 3656 and 3672 by dissolving purified probe DNA in the spotting solution For double stranded DNA prepare solutions containing between 0 10 and 0 20 mg mL For oligonucleotides prepare solutions containing between 0 35 and 0 70 mg mL the molarity of a 0 5 mg mL solution of unmodified 70 mers is 22 uM Set up arrayer and print slides according to manufacturer s or laboratory protocol Always handle slides by the corners and wear powder free gloves 3 Remove arrays from printing platform and place them in original slide container or Corning 25 Slide Holder Cat No 40081 4 Incubate arrays in desiccator for 24 to 48 hours vacuum desiccator works best 5 Optional see note Rehydrate spotted DNA by holding slide array side down over a bath of hot purified water 95 to 100 C for approximately 5 seconds until condensation of the water vapor is observed across th
5. immobilized by UV cross linking When bak ing care should be taken regarding the cleanliness of the oven Volatile organics can irreversibly contaminate the surface of the array leading to high backgrounds Quality and composition of array processing reagents The accuracy and reproducibility of microarray results are highly dependent on the nature of the reagents used to process the arrays Corning strongly recommends the use of the Pronto Universal Hybridization Kit Cat No 40026 40028 which is specifically tuned to the market leading Corning micro array substrates and is meant to provide instant expertise and a standardized trouble free microarraying experience The Pronto system includes the Long Oligo cDNA Hybridization Solution which as its name indicates is for mulated for hybridization to arrays of both long oligonu cleotides and double stranded DNA The reagent formula tions and processes described in this manual have historically performed well for arrays made on aminosilane coated slides but they significantly differ from those of the Pronto Reagent System When deciding which reagents to use it is also important to consider that reagents made in the labora tory environment generally lack the stringently controlled consistency that characterizes the Pronto Reagents Background fluorescence The sensitivity specificity and reproducibility of microarray hybridization are negatively affected by background fluores
6. 9500 f 82 2 796 9300 Singapore t 65 6733 6511 f 65 6735 2913 Taiwan t 886 2 2716 0338 f 886 2 2716 0339 EUROPE France t 0800 916 882 f 31 20 659 7673 Germany t 0800 1011153 f 0800 101 2427 The Netherlands amp All Other European Countries t31 0 20 655 79 28 f 31 0 20 659 76 73 United Kingdom t 0800 376 8660 f 0800 279 1117 LATIN AMERICA Brasil t 55 11 3089 7420 f 55 11 3167 0700 Mexico t 52 81 8313 8586 f 52 81 8313 8589 UltraGAPs and Pronto are trademarks of Corning Incorporated Corning NY Gy is a registered trademark of Amersham plc Buckinghamshire England LifterSlip is a trademark of Apogent Portsmouth NH Corning Incorporated One Riverfront Plaza Corning NY 14831 0001 8 05 BF CLS GAPS 004REV2 2005 Corning Incorporated Printed in USA
7. UltraGAPS Coated Slides Instruction Manual Life Sciences CORNING Discovering Beyond Imagination a For Research Use Cat No 40015 UltraGAPS Slides with Bar Code Cat No 40016 UltraGAPS Slides without Bar Code Cat No 40017 UltraGAPS Slides with Bar Code Bulk Pack Cat No 40018 UltraGAPS Slides without Bar Code Bulk Pack Cat No 40019 UltraGAPS Slide Starter Kit For the most current information about these and related products please visit www corning com lifesciences Rev Jun 2005 CONTENTS Introduction 0 eee 1 QVERVIEW eo ac dai bige aa beh a EAEE aS EA 1 Handling and Care Instructions 2 Storage Instructions 00 eee eee eee 2 Safety Considerations 000000000 2 Product Use Limitations Warranty Disclaimer 3 Preparation and Hybridization of DNA Microarrays 4 General Considerations 000 0000s 4 Array Fabrication and Stabilization 7 Array Hybridization 00 00 cece eee eee 9 Preparation of Hybridization Solution 9 Pre Hybridization 0 00 e eee eee eee 12 Hybridization is ia wersadnet ewan nidi ara Rana 12 Post Hybridization Washes 13 Additional Information 000 14 Customer Service and Technical Support 14 Corning Microarray Products 5 15 INTRODUCTION Overview Corn
8. ar Code 25 25 tures higher than 42 C however calls for changes in the com Bulk Pack position of the hybridization and wash solutions described in 40019 UltraGAPS Slide Starter Kit with 5 mL Universal 10 10 this manual such as exclusion of formamide to properly adjust Spotting Solution their stringency to the requirements of the application at hand 40024 Pronto Universal Validation Kit 1 1 40026 Pronto Universal Hybridization Kit for 25 Arrays 1 1 ADDITIONAL INFORMATION 40027 Pronto Universal Spotting Solution 250 mL 1 1 40029 Pronto Background Reduction Kit for 50 Arrays 1 1 Customer Service and Technical Support 40055 Pronto Plus Direct System for 25 Reactions i 1 1 For a detailed troubleshooting guide answers to frequently with RNA Isolation asked questions and additional information about these and 40056 Pronto Plus Direct System for 25 Reactions 1 1 other products please visit www corning com lifesciences without RNA Isolation i wee ee ee O manual please e mail clstechserv corning com or call sat RNA Isolation 800 492 1110 1 978 635 2200 outside Canada and USA 40076 Pronto Plus Indirect System for 25 Reactions 1 1 without RNA Isolation 40080 Hybridization Chamber II 1 5 40001 Hybridization Chamber O rings 5 5 40081 Corning 25 Slide Mailer 20 20 40082 Corning 5 Slide Mailer 50 50 40085 Microarray Storage Pouch for 5 Slides 50 50 40086 Miroarray Storage Pouch for 25 Slides 50 50 2870 22 Corn
9. cence Depending on their age the storage conditions and the purity of the biological material and other components of the spotting solution used DNA microarrays may develop high levels of background fluorescence on and around the printed areas decreasing the specificity of the hybridization signals The occurrence of spotted fluorescence can be minimized by placing arrays in a Corning 25 Slide Holder Cat No 40081 and storing them in a Microarray Storage Pouch Cat No 40086 This form of background fluorescence can be eliminated by pro cessing the arrays with the presoaking reagents included in the Pronto Universal Hybridization Kit Cat No 40026 40028 The spurious attachment of labeled DNA to the unprinted area of the slide causes high background that interferes with spot identification during data collection and limits the sensitivity and dynamic range of the array Deactivating and or blocking the unused surface of the slide greatly reduces the incidence of this form of background and can be achieved by processing the arrays with the presoaking and prehybridization reagents conveniently included in the Pronto Universal Hybridization Kit Background reduction reagents are also available separately Cat No 40029 for use by printing facilities as the last step in the array manufac turing process prior to storage and distribution to end users Array Fabrication and Stabilization It is crucially important to fully
10. e slide Snap dry array by placing it array side up on a hot plate for 2 seconds 6 Immobilize spotted DNA For double stranded DNA use a UV crosslinker to apply 150 to 300 mJ of UV energy Alternatively bake the arrays at 80 C for 2 to 4 hours If baking place arrays in lidded glass container and make sure oven is clean and free of volatile organics For oligonucleotides use a UV crosslinker to apply 600 mJ of UV energy Baking does not work well for oligonucleo tide arrays 7 Place arrays in Corning 25 Slide Holder Cat No 40081 Place holder containing arrays in Corning Microarray Storage Pouch Cat No 40086 and heat seal pouch Hybridize arrays within 6 months of fabrication Exchang ing the regular atmospheric air for clean nitrogen gas helps prevent oxidation of spotted material and extends the shelf life of the arrays Note Rehydration and snap drying have historically been done to denature double stranded DNA spotted in a non denaturing medium and to evenly distribute the probe DNA within the spotted area The efficacy of this treatment has not been con clusively demonstrated If performing this step utmost care must be taken not to overexpose the array to the hot plate since doing so will affect the integrity of the spotted DNA and increase background Do not place arrays in boiling water as this may result in a significant loss of probe DNA and delamination of the coating Array Hybridization This ins
11. f misused Proper care should be exercised with this product to prevent human contact Corning products are guaranteed to perform as described when used properly Manufacturer liability is limited to the replacement of the product or a full refund Any misuse of this product including failure to follow proper use protocols is the responsibility of the user and Corning makes no warran ty or guarantee under these circumstances Certain arrays and or methods of preparation analysis or use may be covered by intellectual property rights held by others in certain countries Use of this product is recommended only for applications for which the user has a license under propri etary rights of third parties or for technology for which a license is not required Corning s products may be used in connection with the manufacture use and or analysis of oligonucleotide arrays under patents owned by Oxford Gene Technology Limited or related companies OGT but Corning does not have the right to pass on a license under any such patents Therefore before Corning s products can be used in connection with the manufacture use or analysis of oligonucleotide arrays the user should first check with OGT as to whether a license is necessary and if so secure one To inquire about a license under OGT s oligonucleotide array patents please contact licensing ogt co uk For information about OGT please visit its website at www ogt co uk PREPARATION
12. ing UltraGAPS Coated Slides are the substrate of choice for fabricating DNA microarrays of the highest and most consistent quality UltraGAPS Coated Slides are recom mended for the fabrication of arrays of double stranded DNA such as purified PCR products long oligonucleotides and when substrate stability and consistency are absolute require ments UltraGAPS Coated Slides have a uniform covalently bound coating of pure Gamma Amino Propyl Silane GAPS The GAPS coating is applied to both sides of the slides using a proprietary process under tightly controlled manufacturing conditions UltraGAPS slides offer a printing surface of unmatched cleanliness high DNA binding capacity uniformity and stability Microarray quality is highly dependent on the quality and integrity of the printing substrate Arrays printed on coated glass of poor quality are likely to produce spots of varying size shape and DNA content The presence of scratches haze and contaminating particulates on the slide surface also cause deformation of the arrays as well as high background fluores cence These problems lead to loss in sensitivity and generally poor results UltraGAPS Coated Slides are manufactured under the most stringent conditions to prevent these problems All slides are cleaned and individually examined for mechanical defects and for the presence of dust and glass particles GAPS is applied in an environmentally controlled HEPA filtered ISO C
13. ing Cover Glass Square 22 x 22 mm loz 10 packs No 11 2 2940 244 Corning Cover Glass Rectangular 24 x 40 mm loz 10 packs No 11 2 2940 246 Corning Cover Glass Rectangular 24 x 60 mm loz 10 packs No 11 2 Corning Microarray Products continued NOTES Cat No Product Description Qty Pk Qty Cs 3357 96 Well V bottom Polypropylene Microplate 25 100 3656 384 Well Polypropylene Storage Microplate 25 100 3672 384 Well Microarray Printing Plate Low Volume 10 50 3099 Universal Lid Rigid Lid for 96 and 384 Well 25 50 Microplates 6569 Aluminum Sealing Tape for 384 Well Blocks and 100 100 Microplates 6570 Aluminum Sealing Tape for 96 Well Blocks and 100 100 Microplates 3085 DMSO Resistant Lid for 384 Well Microplates 25 50 Corning Incorporated Life Sciences 45 Nagog Park Acton MA 01720 t 800 492 1110 t 978 635 2200 f 978 635 2476 www corning com lifesciences Corning is a registered trademark of Corning Incorporated Corning NY Discovering Beyond Imagination and Flame of Discovery design are trademarks of Corning Incorporated Corning NY Worldwide Support Offices ASIA Australia t 61 2 9416 0492 f 61 2 9416 0493 China t 86 21 3222 4666 f 86 21 6288 1575 Hong Kong t 852 2807 2723 f 852 2807 2152 India t 9111 341 3440 f 9111 3411520 Japan t 81 0 3 3586 1996 f 81 0 3 3586 1291 Korea t 82 2 796
14. lass 5 facility resulting in coated slides with highly uniform surface properties and low intrinsic autofluorescence Surface wetability is consistent across the slide surface to assure uniform spot size and shape and to avoid uncontrolled wicking or poor volume transfer during the printing process Amine density is also uniform across the slide surface leading to consistent DNA retention across the printed array The packaging has been developed to ensure compatibility with the GAPS coating and to maintain the appropriate storage environment Handling and Care Instructions To maximize the benefits of using Corning premium substrates please follow these recommendations Use the slides in a clean environment Particles falling onto the slide surface may cause defects in the printed array as well as nuclease contamination Self contained printing envi ronments may be required to prevent such contamination Avoid direct contact with the surface of the slide Only the print pins and processing solutions should touch the print area to avoid contamination and abrasion of the coating When using slides without bar codes clearly mark the side to be printed using a glass etching tool If the package of slides has been inadvertently stored at temperatures lower than 20 C allow it to come to ambient temperature 20 to 25 C before opening Otherwise con densation may form on the slide surface negatively affecting the uniformit
15. ned by amplification of cloned genomic DNA and cDNAs It is important to purify the amplified fragments as the presence of primers and other components of the reaction mix may interfere with binding to the slide and produce background fluorescence upon hybridization Be sure to use purification methods that do not contribute fluorescent materials Only oligonucleotides of the highest quality should be used for microarraying The optimal length of oligonucleotides to be arrayed on UltraGAPS slides for transcriptional profiling is 70 nucleotides As the GAPS coated surface provides free amine groups for ionic attachment of the negatively charged phosphate groups of the DNA backbone functionalization of the oligonucleo tides with an amine or other reactive group is not necessary Oligonucleotides with and without such modifications bind equally efficiently to the UltraGAPS substrate Concentration of probe DNA The high reactivity of UltraGAPS slides allows the use of dilute printing solutions The optimal concentration needs to be determined empirically When too little DNA is used the DNA spots will not reach signal satu ration levels thus reducing the dynamic range of the array Conversely highly concentrated printing solutions can pro duce spots with comet tails and other forms of localized background The concentration and purity of the DNA should be checked spectrophotometrically as well as electro phoretically The recommended
16. o purified water and incubate at ambient temperature for 30 seconds 7 Dry arrays by blowing high purity nitrogen over the array or by centrifugation at 1 600 rpm for 2 minutes Keep arrays in a dust free environment while completing the preparation of the hybridization solution Hybridization 1 Wash the required number of pieces of Corning Cover Glass Cat No 2870 22 2940 244 2940 246 with nuclease free water followed by ethanol Dry cover glass by blowing high purity compressed nitrogen or allow to air dry in a dust free environment 2 Carefully pipette the target DNA onto the arrayed surface Avoid touching the array with the pipette tip and intro ducing air bubbles Carefully lower the cover glass onto the array Avoid trapping air bubbles between the array and the cover glass Small air bubbles that do form usually dissipate during hybridization Transfer array cover glass assembly to Corning Hybridization Chamber II Cat No 40080 3 Assemble the chamber as described in the Corning Microarray Hybridization Chamber Operating Instructions Manual Keep the chambers right side up and in a horizon tal position at all times to prevent movement of the cover glass relative to the array 4 Submerge chamber array assembly in a water bath or place in a hybridization oven kept at 42 C 5 Hybridize arrays at 42 C for 12 to 16 hours Post Hybridization Washes It is extremely important not to allow the arrays to dry out
17. pL of hybridization solution per cm of surface area When using a raised edge coverslip apply 3 pL per cm 3 Calculate the amount of target DNA needed for each array The fluorescence strength required to achieve high levels of sensitivity and broad dynamic range depends on the type of RNA used to synthesize the target cDNA For Cy cDNA made from mRNA use an amount of tar get DNA containing 0 25 pmoles of Cy nucleotides per microliter of hybridization solution per dye For example to hybridize an area covered by one Corning 22 x 22 mm cover glass approximately 5 cm dissolve an amount of cDNA containing 3 pmoles of each Cy3 and Cy5 dNTP in 12 pL of hybridization solution For Cy cDNA made from total RNA use an amount of target DNA containing 1 0 pmoles of Cy nucleotides per microliter of hybridization solution per dye For example to hybridize an area covered by one Corning 22 x 22 mm cover glass approximately 5 cm dissolve an amount of cDNA containing 12 pmoles of each Cy3 and Cy5 dNTP in 12 pL of hybridization solution For Cy genomic DNA CGH applications use an amount of target DNA containing 1 0 pmoles of Cy nucleotides per microliter of hybridization solution per dye For exam ple to hybridize an area covered by one Corning 22 x 22 mm cover glass approximately 5 cm dissolve an amount of DNA containing 12 pmoles of each Cy3 and Cy5 dNTP in 12 pL of hybridization solution 4 Dissolve the ap
18. propriate amount of target DNA in the required volume of hybridization solution 5 Incubate the DNA hybridization solution at 95 C for 5 minutes 6 Briefly centrifuge the cDNA hybridization solution to col lect condensation and allow it cool to room temperature Do not place the solution on ice as this will cause precipi tation of some of the components Protect the labeled DNA from over exposure to light to minimize photobleaching Pre Hybridization Prehybridization should be done immediately preceding the application of the target cDNA onto the arrays This step has the purpose of blocking the unused surface of the slide and removing loosely bound probe DNA It is recommended that all target DNAs be characterized prior to the start of pre hybridization The preparation of the hybridization solutions can be completed during the time arrays are being pre hybridized 1 Prepare prehybridization solution consisting of 5 x SSC 0 1 SDS and 0 1 mg mL BSA The volumes required to process a given number of arrays depends on type of glassware available Use Coplin jars to simultaneously process up to 5 arrays using only 50 mL of buffer per step 2 Warm prehybridization solution to 42 C 3 Immerse arrays in prehybridization solution and incubate at 42 C for 45 to 60 minutes 4 Transfer prehybridized arrays to 0 1 x SSC and incubate at ambient temperature 22 to 25 C for 5 minutes 5 Repeat Step 4 6 Transfer arrays t
19. truction manual describes labeling parameters and hybridization protocols related to the use of arrays of amplified cDNA clones and arrays of oligonucleotides for measuring relative transcript abundance transcriptional profiling and studying chromosomal abnormalities comparative genomic hybridization For transcriptional profiling we recommend the use of the Pronto Plus Systems Cat Nos 40055 and 40056 for direct labeling and 40075 and 40076 for indirect labeling which include reagents for RNA isolation cDNA synthesis and array hybridization Please refer to Comparative Genomic Hybridization Using Corning Products Application Report www corning com lifesciences for information about hybridization of arrays of BAC derived double stranded DNA Preparation of Hybridization Solution The quality and purity of the template RNA and the resulting cDNA are critical factors for successful hybridizations Deter mine the yield and purity of the template RNA by measuring absorbance at 260 and 280 nm and by gel analysis Use only RNA showing a 260 280 ratio between 1 7 and 2 1 After syn thesis and purification of the cyanine labeled target cDNA measure absorbance at 260 550 and 650 nm Best hybridiza tion results are obtained with cDNA having a frequency of incorporation FOT of at least 20 labeled nucleotides per thousand Using cDNA of lower FOI reduces the sensitivity of the assay An FOI greater than 50 is indicati
20. ve of incomplete removal of unincorporated labeled nucleotides Determine the yield and label strength of target cDNA using the following formulae Amount of target DNA ng A260 x 37 x total eluted volume pL Labeled nucleotides incorporated pmoles for Cy3 Assy X total eluted volume 0 15 for Cy5 Asso X total eluted volume 0 25 FOI Labeled nucleotides incorporated x 324 5 amount of target DNA Note When labeling genomic DNA subtract the amount of template genomic DNA to calculate actual yield Note These equations were generated using the following constants One Azs unit of single stranded DNA 37 pg mL extinction coefficient of Cy3 150 000 M em at 550 nm extinction coefficient of Cy5 250 000 M em at 650 nm average molar mass of dNTP 324 5 1 Prepare fresh hybridization solution consisting of For arrays of Jong oligonucleotides 20 to 35 formamide 5 x SSC 0 1 SDS and 0 1 mg mL of a nucleic acid blocker such as sonicated salmon sperm DNA or calf thymus DNA For arrays of cDNA derived double stranded DNA 35 to 50 formamide 5 x SSC 0 1 SDS and 0 1 mg mL of a nucleic acid blocker such as sonicated salmon sperm DNA or calf thymus DNA 2 Determine the area of the slide to be exposed to the hybridi zation solution and calculate the volume of hybridization solution needed for each array When using Corning Cover Glass Cat No 2870 22 2940 244 and 2940 246 apply 2 5
21. y of the coating Open the pouch just prior to printing Close the cap on the slide container as soon as possible after removing slides to maintain a closed environment for unused slides Place the closed container in the pouch to protect the remaining slides and store them in a desiccator Use the remaining slides within one week of opening the pack Storage Instructions Store UltraGAPS slides at ambient temperature in original undamaged packaging and use slides by the date indicated on the label Proceed as described in the Handling and Care Instructions after opening the package Safety Considerations When working with the UltraGAPS slides please follow all generally accepted laboratory safety guidelines At a minimum wear the appropriate personal protective equipment such as a lab coat safety glasses powder free gloves etc Follow recom mended standard operating procedures for any laboratory equipment used in your experiments Read the Material Safety Data Sheet MSDS for appropriate handling of all products MSDS is available upon request or can be downloaded from www corning com lifesciences Product Use Limitations Warranty Disclaimer Corning UltraGAPS Coated Slides are sold for research purposes only and are not intended for resale This product is not to be used in human diagnostics or for drug purposes nor is it to be administered to humans in any way This product contains chemicals that may be harmful i
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