Model Pico Plus Syringe Pump User`s Manual
Contents
1. Reversing the Flow Direction The pump is equipped with a reversing switch to toggle from infuse to with draw The flow rate will be the same for both infuse and withdraw modes unless changed manually refer to 4 CAUTION Remember to check the switch before pressing run to ensure it is indicating the desired direction Maintenance Keep the pump clean and dry Avoid liquid spills that may find their way into the electronics A small container of grease is provided for periodic lubrication of the lead screw and guide rods It is important to keep these guide rods clean and lubri cated To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the display window For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 75 isopropyl alcohol If the pump does not work properly contact Harvard Apparatus for appropri ate instructions Protecting Small Fragile Syringes The pico plus will hold microliter size syringes down to 0 5 pl size These small syringes have fine wire plungers that may be damaged if allowed to bot tom out Be sure to set the limit switches properly to prevent damage to small syringes Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Appendix A Type Syringe Size for Pico Plus with Holder For 2 Syringes Flow Rate Range Minimum Maximum Calibration Display Nonvolatile Memory Maximu2. Read all labels on product to ensure proper usage CAUTION Functional Ground Refer to Manual _ Terminal Environmental Conditions Indoor use only Temperature 4 C to 40 C 40 F to 104 F Humidity 20 to 80 RH non condensing Well Ventilated Room Altitude up to 2000 m Mains Voltage Fluctuation not to Exceed 10 of Nominal Transient Over Voltage Category II Pump is Rated Pollution Degree 2 in Accordance with IEC 664 Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Introduction Theory of Operation The pico plus is designed as a low cost infusion pump capable of delivering very low flow rates at low to moderate back pressures The pump can hold two syringes of any make from 0 5 pl to 10ml The diameter of the syringes is entered via the keypad and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow Non volatile memory stores the last syringe diameter and flow rate along with other configuration data The Power Failure Mode can be set to either turn the pump off in the event of power failure or to resume pumping when power resumes Features Bright Display and Easy To Use Interface A new two line 16 character display along with six membrane keys make this a most attractive but powerful easy to use syringe pump Only two entries are required to start pumping syringe Inside Diameter mm and pumping flow rates The Flow rate can be cha
3. manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Power Supply The pump is supplied with an approved power supply and line cord To maintain the safety integrity of the device use only one of the following power supplies Ault Inc Model No PW118 Output 12 Vdc 1 5 A Input 100 250 Vac 50 60 Hz 5A Cui Stack Model No SA06N12 V Output 12 Vdc 2 0 A Input 100 240 Vac 50 60 Hz 8A Globtek Inc Model No GT 4201D 12 Output 12 Vdc 1 66 A Input 100 240 Vac 50 60 Hz 0 6A Use Proper Line Cord Use only the line cord shipped with the product and make sure line cord is certified for country of use Ground the Product This product is grounded through the return path of the DC power supply To avoid electric shock use only approved power supply and line cord with the product Make Proper Connections Make sure all connections are made properly and securely Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump General Safety Summary Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Observe all Warning Labels on Product
4. stop or run Press the ENTER key to select either mode Exit the CONFIG mode by pressing the ENTER key again and save the POWER ON setting in memory Publication 5412 001 REV E Operation Advanced Features 3 A va Harvard Apparatus Pico Plus Syringe Pump Display Intensity For varying light conditions four levels of intensity can be set on the vacuum fluorescent display To set the desired display intensity at the RATE VOL CONFIG display move the underline cursor using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET INTENSITY Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the SET INTENSITY choices 1 2 3 4 4 is highest intensity 1 is the lowest intensity Press the ENTER key to select the desired display intensity Exit the CONFIG mode by pressing the ENTER key again and save the INTENSITY setting in memory Changing Rates If the pump is running at an existing rate it will continue to do so until a new rate is entered Except for volume mode the flow rate can be changed while the pump is running As soon as the ENTER key is pressed the pump will change to the new flow rate To change rates from the keypad while in vol ume mode the pump must be stopped first The pump must be stopped to change the flow rate range units while in either mode
5. place to the right Once the desired rate is displayed press the ENTER key to place this value into memory Press RUN Or Foot Switch Press the RUN STOP key or press the foot switch to start pump and begin pumping The Run Indicator Green LED above the RUN STOP key will light when the pump is on and pumping Check Syringe Often The pico plus pump will shut itself off when the syringe is empty via the infuse limit switch If the switch is improperly set or the syringe is overloaded the pump will not shut itself off Although this presents no hazard to the user or the pump it is prudent to check the syringe from time to time Publication 5412 001 REV E Operation Advanced Features 1 Harvard Apparatus Pico Plus Syringe Pump 2 Volume Mode The pico plus can be set to dispense a precise volume and then stop To acti vate the volume dispense mode a target volume must be set To set a target volume at the RATE VOL CONFIG display move the under line cursor using the A or V key to VOL Press the SET key to enter the VOL set mode Use the A or V key and the SET key to display a target volume from 00 01 to 99 99 Volume units are pl picoliters nl nanoliters ml mil liliters or pl microliters Target volume units are established in the CONFIG SET UNITS mode example if your pumping units are ml min or ml hr then the volume dispense units will be ml s Press the ENTER key to select the desired target volume Exi
6. the left most digit or decimal point The A and V keys are used to scroll to the desired number and the SET key moves the underline cursor one place to the right Once the desired diameter is displayed press the ENTER key to place this value into memory Enter Flow Rate Range Choose your flow rate units picoliters nanoliters microliters or milliliters per minute or per hour From the initial RATE VOL CONFIG menu using the A or V key move the underline cursor under the CONFIG mode menu prompt Press the SET key to enter the CONFIG mode Press the SET key again to move the under line cursor to the flow rate choices Choose your flow rate units while in the SET UNITS mode by pressing the A or V keys to scroll the four flow rate choices ml min pl min ml hr pl hr Once the desired flow rate units are dis played press the ENTER key to return to the main SET CONFIG mode Press SET or ENTER again to put your desired flow rate units into memory and return to the RATE VOL CONFIG menu Enter Flow Rate From the initial RATE VOL CONFIG menu press the SET key to enter the SET RATE mode Each time you change the syringe diameter the previously used flow rate is erased If the syringe diameter is unchanged the previously used flow rate will appear on the display The underline cursor will appear under the left most digit or decimal point The A and V keys are used to scroll to the desired number and the SET key moves the underline cursor one
7. 5 valid only in the U S outside U S call 508 893 8999 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replace ment and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair C AUTI O N parts When ordering please describe parts as FOR RESEARCH USE ONLY completely as possible preferably using our part NOT FOR CLINICAL numbers If practical enclose a sample or drawing USE ON PATIENTS We offer a complete reconditioning service CAUTION This pump is not registered with the FDA and is not for clinical use on human or veterinary patients It is intended for research use only Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump General Safety Summary Please read the following safety precautions to ensure proper use of your syringe pump To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment is used in a manner not specified by the
8. 70 5 648 13 80 26 76 67 41 134 1 269 4 668 4 1344 2686 6696 9481 9999 0 0778 0 1722 0 3389 0 8278 1 606 4 044 8 043 16 16 40 11 80 61 161 2 401 8 568 9 805 6 1610 A table of minimum and maximum diameter values pL hr max diameter pL mn max diameter 1 14 8 89 mL mn min diameter 0 22 Publication 5412 001 REV E 1241 2794 5477 9999 0 0013 0 0029 0 0056 0 0138 0 0268 0 0674 0 1341 0 2694 0 6684 1 344 2 686 6 697 9 482 13 43 26 83 20 69 46 57 91 28 225 4 437 9 1103 2196 4412 9999 0 1612 sf Appendix C Flow Rates 0 0001 0 0003 0 0004 0 0009 0 0017 0 0041 0 0081 0 0162 0 0402 0 0806 2637 0 4018 0 5689 0 8056 1 610 Publication 5412 001 REV E 1 241 2 794 5 477 13 52 26 28 66 17 131 7 264 7 656 7 1317 0 0027 6580 9313 9999 0 0001 0 0002 0 0003 0 0007 0 0014 43 94 0 0067 0 0095 0 0135 0 0269 0 2254 0 4379 1 103 2 196 4 412 10 94 21 94 0 0002 109 7 155 2 219 6 439 4 0 0001 2 637 0 0005 0 0006 0 0009 0 0017 0 0011 0 0027 0 0054 0 0135 0 0262 0 0661 0 1317 0 2647 0 6567 1 317 6 580 9 313 13 16 26 36 0 0439 0 0001 0 0002 0 0002 0 0004 0 0011 0 0021 0 0044 0 0109 0 0219 0 1097 0 1552 0 2196 0 4393 Harvard Apparatus Pico Plus Syringe Pump Appendix D Serial Communication Serial connector RJ 11 style pinning Position Signa
9. All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibration All syringe pumps are designed and manufactured to meet their performance spec ifications at all rated voltages and frequencies A calibration certificate is available upon request Contact customer service for details and pricing Warranty Harvard Apparatus warranties this instrument for a period of two year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCI DENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclu sion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact your local distributor or Harvard Apparatus 84 October Hill Road Holliston Massachusetts 01746 1388 using our toll free number 1 800 272 277
10. Model Pico Plus Syringe Pump User s Manual Pump 11 Pico Plus MA1 70 2213 HARVARD APPARATUS Publication 5412 001 REV E WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any chang
11. N PATIENTS Power Switch Foot Pedal Reversing Receptacle Serial Label Power Cord Toggle Switch Receptacle Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Installation Initial Set up Read the manual to become familiar with all features and functions of the pico plus Connect the external DC power supply and line cord to the pump and main supply Turn on main power switch located on the rear panel The display will now illuminate indicating that the power connections are correct The display will indicate POWER FAIL this is normal as the pump indicates on the display if power was disrupted since last use Location Requirements for the Syringe Pump e A sturdy level clean and dry surface e Minimum of one inch 2cm clearance around the pump e Appropriate environmental conditions e A well ventilated room Loading the Syringe i 10 11 Press the bronze halfnut button 1 on the side of the pusher block to release it from the leadscrew While holding the button in slide the pusher block 2 to the left Loosen plunger retaining bracket 3 and syringe retaining bracket 4 by slightly unscrewing the cap screws Loosen the thumbscrew on the syringe clamps 5 and rotate the clamp out of the way Lay the syringe into the V shaped slots on the syringe holder block 6 Swing the syringe clamps 5 over the syringes and tighten the thumbscrews to secure th
12. Syringe Inside Diameter Terumo Size Diameter 3 cc 8 95 mm 5 13 00 10 15 80 1 cc 4 78 mm Sherwood Monoject Plastic Size Diameter 1 cc 4 65 mm 3 8 94 6 12 70 12 15 90 Cadence Science Inc Formly Popper amp Sons Inc Perfektum Glass Size Diameter 0 25 cc 3 45 mm 0 5 3 45 1 4 50 2 8 92 3 8 99 5 11 70 10 14 70 Publication 5412 001 REV E Stainless Steel Size Diameter 8 cc Becton Dickinson Plastic Plasticpak Size Diameter Tec 4 78 mm 3 8 66 5 12 06 10 14 50 Air Tite All Plastic Size Diameter 2 5cc 9 60 mm 5 0 12 45 10 15 90 Unimetrics Series 4000 amp 5000 Size Diameter 10 pl 0 460 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 9 525 mm GE Scientific Glass Engineering Size Diameter 25 pl 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0ml 4 61 mm 2 5 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size Diameter 5 pl 0 103 mm 1 0 1457 2 0 206 5 0 3257 10 0 460 25 0 729 50 1 031 100 1 46 250 2 3 500 3 26 1 0ml 4 61 mm 2 5 7 28 5 10 3 10 14 57 Harvard Apparatus Pico Plus Syringe Pump Appendix C Flow Rates Syringe Min tul 2ul 5ul 10ul 25ul 50ul 100ul 250ul 500ul 1ml 1000p1 2 5m 3ml 5ml 10ml pl hr ID mm min max 0 1 0 15 0 21 0 33 0 46 0 73 1 03 1 46 2 3 3 26 4 61 7 28 8 66 10 3 14 57 77 78 9999 172 2 338 9 827 8 1606 4044 8043 1 296 2 8
13. e syringe in place Slide the syringe retaining bracket 4 up to secure the flange on the syringe barrel against the syringe holder block Press the halfnut button 1 and hold it in while sliding the pusher block up to the syringe plunger Tighten the thumbscrews on the plunger retaining bracket 3 The infuse limit switch is adjusted by loosening the thumbscrew on the back of the pusher block and sliding the stop pin 7 in or out to the desired loca tion to protect the syringe from bottoming out The withdraw limit switch enclosed in the cover is adjusted by securing the stop collar 8 into the desired position Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Operation Getting Started Getting started Turn Pump ON Turn on power using the switch on rear of the pump the display will light and indicate POWER FAIL this is normal as the pump indicates on the dis play if power was disrupted since last use 2 Function Keys and Run Indicator Refer to the colored keypad at the front of the pump to identify the following functions starting from the right nunsce RUN STOP This turns the pump motor on and off also accomplished by pressing the foot switch ENTER This key enters the data that is on the display into the memory of the pump Also used to query the flow rate DIAM Used to enter or query the syringe diameter SET This key is used to
14. es as they apply Do Not Dispose Product with Municipal Waste e Special Collection Disposal Required Harvard Apparatus Pico Plus Syringe Pump Table of Contents General Information Warranty and Repairs 2 General Safety SUMMAFY criiiiiiiiii 3 4 Introduction Theory of Operation i 5 Features prude 5 Front and Rear ViewWS 0 1 1 rrrrriiiiiiiiiiniin 6 Installation Initial Setup amp Location Requirements 7 Loading the Syringe i 7 Operation Getting Started TUR PUMP ON cola 8 Function Keys and Run Indicator ii 8 Entering Syringe SIZE cia 9 Entering Flow Rate Range 9 Entering FIOW Rate iaia 9 Press Run Footswitch iiiii 9 GACCKSVIANGE oat r EE ria redatta 9 Advanced Features Volume Mode 53 ctainsiaa cay shi karian eaa 10 Power Failo sismi i iaa 10 Changing Rates Aia 11 MAINTENANCE lil aa 11 Protecting Small Fragile Syringes srirrirrirerisiaras 11 Appendices A Specifications i 12 B Table of Popular Syringe Diameters 13 C Table of Minimum and Maximum Flow Rates 14 D Serial Communication eee eeestteeeeeeeaees 16 19 E R9232 Connections iii 20 E PGCONNECHONI iii 20 Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump General Information Serial Number
15. keyboard control Exit remote mode RUN Start infuse forward direction STP Stop motor CLV Clears volume accumulator to zero CLT Clears target volume to zero dispense disabled REV Start Reverse Direction Commands with numbers MMD number Set syringe diameter units are mm Rate is set to zero after MMD command PLM number Set flow rate and range units are Picoliters per minute NLM number Set flow rate and range units are Nanoliters per minute ULM number Set flow rate and range units are microliters per minute MLM number Set flow rate and range units are milliliters per minute PLH number Set flow rate and range units are Picoliters per hour NLH number Set flow rate and range units are Nanoliters per hour ULH number Set flow rate and range units are microliters per hour MLH number Set flow rate and range units are milliliters per hour TGT number Set target infusion or refill volume units are set in the flow rate range choosen Numbers can be between 0 and 1999 Leading zeros and trailing decimal point are optional Any number of digits to the right of the decimal point may be transmitted The number will be rounded Publication 5412 001 REV E Appendix D Serial Communication a Queries DIA Returns diameter value units in mm oO RAT Returns rate value set in current range units D none c VOL Returns current accumulated infused volume units in ml or pl T depending on range gt n VER Returns model and
16. l 11 Plus Straight Thru i Pi Syringe Pump Ae 15M Computer Terminal Port Settings Baud Rate 9600 or match pump baud rate Data Bits 8 Stop Bits 2 RJ11 6x4 View of Cable Publication 5412 001 REV E DB9 Female to Computer 4 6 PIN RJ11 12 such as L COM P N RBA098F Front View Connection Chart STD DB9 RD 3 TD Sig ground Adapter Declaration of Conformity Application of Council Directive s 73 23 EEC 89 336 EEC Standard s to which conformity is declared EN61010 1 1993 A2 1995 EN 61326 1997 A1 1998 Class A Emissions Class B Immunity Manufacturer s Name Harvard Apparatus Inc Manufacturer s Address 84 October Hill Road Holliston Massachusetts 01746 U S A Type of Equipment Syringe Pump Model No 70 2213 Pico Plus I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s Place United States of America Date September 06 2002 C Signature HARVARD Be APPARATUS VP Engineering Operations Position
17. l 1 GND 2 GND 3 TxD 4 RxD e The connector position as viewed looking into the RJ 11 connector reading left to right Baud rate and address The baud rate and address are set in the CONFIG mode options are described as follows a Pump Address To set the pump address at the RATE VOL CONFIG display move the underline cursor using the W or A key to CONFIG Press the SET key to enter the CON FIG mode options Use the W or A to scroll the CONFIG options until you reach the SET ADDRESS Press the SET key again moving the underline cursor to the right Use the W or A to scroll the two digit number fields Use the W or A key and the SET key to display a unique pump address from 00 to 99 Press the ENTER key to select the desired pump address Exit the CONFIG mode by press ing the ENTER key again and save the ADDRESS setting in memory b Baud Rate To set the pump address at the RATE VOL CONFIG display move the underline cursor using the W or A key to CONFIG Press the SET key to enter the CON FIG mode options Use the W or A key to scroll the CONFIG options until you reach the SET BAUD Press the SET key again moving the underline cursor to the right Use the V or A key to scroll the SET BAUD choices 300 1200 2400 or 9600 Press the ENTER key to select the desired baud rate exit the CONFIG mode by pressing the ENTER key again and save the BAUD setting in memory Publication 5412 001 REV E Harvard Ap
18. m Force Maximum Pressure Drive Motor Step Rate Minimum Maximum Pusher Advance Step Pusher Travel Rate Minimum Maximum Dynamic Range Leakage to Ground Ground Resistance Input Power Input Power Connection Power Supply Size HxWxD Weight Pico Plus VPF Specifications Microprocessor dual syringe infuse withdraw Plastic or glass From 0 5 ul to 10 ml 1 3 pl min with 0 5 pl syringe 0 4393 ml min with 10 ml syringe 0 8788 ml min using 2 x 10 ml syringes combined output Automatic enter syringe size up to 16 mm Inside Diameter 2 line 16 character vacuum fluorescent display and green run led Stores diameter rate and configuration settings 25 Ib 100 p s i with 10 cc syringe 175 p s i with 1 ml syringe 1 8 step angle geared 36 1 motor 1 pulse in 27 6 sec 200 steps sec 0 0444 um pusher advance per motor step 0 0388 u m min 0 8333 mm min 1 to 16 384 Typically lt 150 pA Typically lt 0 05 ohms 12 VDC 1 5 Amps 2 5mm ID x 5 5 mm OD male plug 100 250 VAC 50 60 Hz 18 Watts External Universal Power Supply Use Only a Harvard Apparatus Approved Power Supply and Line Cord 11 4 x 22 9 x 11 4 cm 4 1 2 x 9 x 4 1 2 in 2 3 kg 5 Ib Additional accessories are required to achieve this flow rate Actual force is higher but not recommended for applications requiring more than 25 lbs of force Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Appendix B
19. nged while the Pump is running Direct enter flowrates in ml pl nl and pl Two Modes of Operation Constant Flow Rate and Volume Dispense The pico plus will operate continuously in RATE mode or accurately dispense a specific amount of fluid in VOLUME mode All modes can be operated from key pad footswitch included or external controller via serial connection included Withdraw mode makes it easy to load capillaries pipettes and syringes Adjustable end of travel limit stop to protect small syringes Smooth Flow Enhanced micro stepping pump profiles deliver very smooth and consistent flow that is virtually pulse free Nonvolatile Memory The pump remembers its last syringe size flow rate used and configuration settings Power Fail Mode In a power failure the Pump can either RESUME or STOP pumping when power is returned CE Mark Approved UL CSA ETL The pico plus meets all relevant European EMC and Safety requirements for lab oratory equipment Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Pico Front and Rear Views Syringe Clamp 5 Syringe Holder 6 Allen Wrench Plunger Retaining Bracket 3 Pusher Block 2 4 Adjustable Stop uu J Run LED Collar 8 Halfnut 1 Display Syringe Retaining Bracket 4 Adjustable Cover Infuse Stop Pin 7 Limit Switch Leadscrew CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE O
20. paratus Pico Plus Syringe Pump Appendix D Serial Communication Operation with Serial control and external Run Stop switch foot switch Mode Function Local Keyboard Run Stop Remote Serial control Run Stop Exit Remote Publication 5412 001 REV E Operation Momentary switch contact If pressed when stopped starts pump motor Run LED lights when running If pressed when running stops pump motor REMOTE mode initiates when pump receives a CR character ASCII 13 REMOTE is displayed on pump display while in remote mode RUN Commands the pump motor to start STP Commands the pump motor to stop KEY Commands the pump to exit REMOTE mode Keyboard Run Stop key while in REMOTE mode will stop pump motor if running Will not restart pump motor in REMOTE mode While in REMOTE mode External Run Stop connection is ignored Appendix D Serial Communication Commands Queries and Responses After each transmission to the pump terminating with a CR character ASCII 13 the pump enters remote mode and responds with the three character sequence CR LF prompt The prompt character indicates the status of the pump as follows prompt meaning ASCII code When stopped ASCII 58 decimal gt When running forward ASCII 62 decimal i When stalled ASCII 42 decimal Pump 11 does not include stall detection Serial commands and their meanings Harvard Apparatus Pico Plus Syringe Pump Commands KEY Return to
21. select which digit of the display is to be changed to move the decimal point and to move between modes Each time the set key is pressed the underline cursor below the digit or character on the display moves one step to the right It is used in conjunction with the ascending and descend ing keys When it the display shows the desired the correct value the set key will advance right to the next digit AV The ascending and descending keys are used to change the numbers on the display A Up key makes numbers increase V Down Key makes numbers decrease When the underline cursor is placed below the decimal point the AV keys shift the decimal one place up or down Run Indicator When the pump is running the highly visible green LED above the RUN STOP key will illuminate 3 Enter Syringe Size Enter the inside diameter ID of the syringe you wish to use Units are in mil limeters mm If you do not know your syringe diameter refer to appendix B for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Operation Getting Started 4 va Press SET followed by the DIAM key The previously used diameter will appear on the display The underline cursor will appear under
22. t the VOL mode by pressing the ENTER key Once you press the RUN key the pump will run until the target volume is delivered The display will show the actual volume dispensed along with the target volume Press the RUN key each time you want to repeat the volume dispense If you press the STOP key during a volume dispense you can restart the pump at the place you stopped by pressing the RUN key again To exit the volume dispense mode set the target volume to 00 00 or turn off and on the pump via the main power switch NOTE In the event of a power failure the actual dispensed volume and the target volume are not retained in memory This means that while in volume dispense mode if a power failure occurred the pump would not resume volume dispense pumping even if the POWER ON mode was set to run Power Failure In the event of a momentary or prolonged power failure the pico plus can be set to either a Resume pumping when power is returned with POWER FAIL on the display b Not start pumping when power is returned with POWER FAIL on the display To set the power fail mode at the RATE VOL CONFIG display move the underline cursor using the A or V key to CONFIG Press the SET key to enter the CONFIG mode options Use the A or V key to scroll the CON FIG options until you reach the SET POWER ON display Press the SET key again moving the underline cursor to the right Use the A or V key to scroll the POWER ON choices
23. version number of firmware E TAR Returns target volume units in ml or pl depending on range 8 value format nnnn nnn o The returned value is an 8 character string with leading zeros converted to SP D characters ACSII 32 decimal The fifth character is a decimal point ASCII 46 decimal o w Queries with string response lt RNG Returns range message character string either ML HR ML MN 2 UL HR UL MN NL HR NL MN PL HR and PL MN o gt Error responses E CR LF CR LF prompt Unrecognized command CR LF OOR CR LF prompt Entered value in out of range Remote Communication Set up The pico plus is equipped at the factory for remote communications The communication format is Baud Rate User settable 300 1200 2400 9600 Parity None Stop Bits 2 Data Bits 8 Duplex Half Address User settable 00 default to 99 Publication 5412 001 REV E Harvard Apparatus Pico Plus Syringe Pump Appendix E Dual RS 232 RXD _ GND TXD _ GND GND _ RXD GND TXD IN OUT Host Chain Computer Connection to Connection Additional Pump s Daisy Chain Connector amp Cables Part No Description 70 2022 RS232 serial cable 6 ft long 9 pin d sub on one end and RJ11 on the other end 72 2478 Daisy Chain Cable 6 ft 55 7760 Daisy Chain Cable 2 ft 72 8340 USB to Serial Adapter Appendix F PC Connection m e Cp RJ11 Cable Mode
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