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Foxy R1 and Foxy R2 Fraction Collectors

1. 603812182 REPLACEMENT PARTS LIST SHEET 10 OF TELEDYNE ISCO FOXY RI REV PART NUMBER DESCRIPTION 26 602133616 DROP FORMER ARM SENSOR FLAG 01 602133620 VALVE HOUSING BACK COVER 28 209016524 4 28 HEADLESS WUT FOR 1 16 OD TIBG 29 20901632 FERRULE W RING FOR 1 16 OD TUBING 30 692133629 VALVE 3 WAY WITH 1 4 28 PORTS 3 602134416 DROP COUNTER amp VALVE JCT CBA ASSY 32 602133618 INTERNAL VALVE COVER 33 602133623 VALVE ADJUSTMENT CLAMP 34 602133619 VALVE COVER 32 602133658 DROP FORMER SLEEVE RETAINER 36 693813015 DROP COUNTER 5 3l 60213360 DROP FORMER 38 2098016319 FERRULE W RING FOR 1 8 OD 39 602134412 TEE KNOB ASSEMBLY 40 231514310 4 40 X 5 8 PANHEAD SCREW 4 692134649 DROP FORMER MOTOR ENCODER CBA 4 231012000 140 X 31 LONG SPACER 43 60213441 DROP FORMER MOTOR ASSEMBLY 44 602134414 DROP FORMER MOTOR W ENCODER CBA ASSY 45 232910100 4 40 LOCKING HEX NUT 46 60213360 ARM SENSOR FLAG 41 692134643 CONNECTOR CBA 48 602134415 ARM MOTOR W ENCODER ASSY 49 602134410 ARM MOTOR ASSEMBLY 50 692134654 A ARM MOTOR ENCODER CBA NOTE For current prices and quotations on parts con
2. 603812162 REPLACEMENT PARTS LIST SHEET 9 OF 11 TELEDYNE ISCO FOXY RI REV C y PART NUMBER DESCRIPTION 231014506 8 32 X 3 8 PANHEAD SCREW 2 233211200 8 INTERNAL TOOTH LOCK WASHER 3 233011200 8 FLAT WASHER 4 05233016 SHORT BLACK FOO 5 93813319 DASE LABE 6 03813228 DRIP TRAY 0100513 32 X 3 8 FLH SCREW BLACK CHERRY 8 038 5054 CPU CBA 9 341000003 POWER SUPPLY 24VDC 0 602133679 CPU CBA FISH PAPER 2 603813209 MAIN ARM TOP COVER 3 23181432 X 25 PANHEAD SCREW 4 233210400 INTERNAL TOOTH LOCK WASHER 5 602133606 DROP FORMER AR OUNTING BLOC 6 602135081 DROP FORMER VALVE RIBBON CABLE 209003302 DROP FORMER AR INEAR RAIL amp SLIDER 8 231014304 4 40 X 4 PANHEAD SCREW 9 233100400 4 SPLIT LOCK WASHER 20 69523412 DROP FORMER ARM OPTICAL SWITCH 2 209003303 MAIN ARM LINEAR RAIL amp SLIDER 22 602133670 DROP FORMER ARM IDLER PULLEY 23 209003033 DROP FORMER ARM DRIVE BELT 24 233010403 4 FLAT WASHER 29 231116306 4 40 X 5 16 CAP SCREW a Ta gt B 10 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings
3. 3 10 3 8 Method Selection Menu 3 10 3 9 Rename Method 1 3 11 3 10 Drop Counts display Ses ss n n kayan eer 3 11 3 11 Time Intervals display WO Taka 8 18 3 12 Volume display au nal a k Ee kn shell ehh 3 14 3 13 Threshold detection 0 0 kk KK KK KK KK KK KK KK KK KK KK KK KK KK KK K k 3 15 3 14 Threshold Settings 3 16 3 15 Peak Width detection 3 17 3 16 Peak Width Settings menu 3 18 3 17 Time Windows limit the collection to specific time durations 3 20 3 18 Time Windows Settings menu 3 21 5 1 Retaining ring for the drop counter s glass sleeve 5 3 1 Configuration settings 6 2 Configuration settings A 7 A 3 Programming displays for the current method A 8 A 4 Saved method recall and method name editing A 9 vii Foxy9 R1 and Foxy R2 Fraction Collectors Table of Contents List of Tables 1 3 Rear Panel Connectors sa 4
4. MAN 6021326626 SHEET OF 12 2 B 13 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix Replacement Parts Listings FOXY DRAWING NO 6021 326620 Xr Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings FOXY R2 DRAWING NO 602132662C SHEET 3 OF 12 B 15 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings DETAIL SCALE 0 500 FOXY R2 DRAWING NO 602132662C SHEET 4 OF 12 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings W3ll 10 Luvd A M au R za BK CN 09 FOXY R2 DRAWING NO 602132662C SHEET 5 OF 12 B 17 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings FOXY R DRAWING NO 602132662C SHEET 6 OF 12 DETAIL SCALE 0 600 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix Replacement Parts Listings DETAIL B SCALE 0 600 FOXY R2 DRAWING NO 602132662C SHEET 7 OF 12 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings FOXY R DRAWING NO 602132662C SHE
5. 4 17 Section 5 Maintenance 5 1 Introductio ee DPI UESTRO bg I epis 5 1 5 2 Maintenance ses eena bw da XAR le ERR SSE Wd A RE EN WR E VR RATS 5 1 5 2 1 Tubing Inspection yak b KT eee 5 1 5 2 2 Collection Rack and Drip Tray Cleaning 5 2 5 2 3 Exterior Cleaning 4 2 0 ne sce sea eo Han Sere E die b n elek HOP goals 5 2 5 2 4 Fluid Path Cleaning 5 2 5 2 5 Drop Counter Cleaning 5 3 5 9 CL VICE s AS Los e ee cte i wal abe ire hydra acie Se ee RE Se es 5 4 5 4 Instrument Returns tonat CE do aer eee ea USUS 5 4 5 4 1 How to Ship Returns 5 4 Appendix A Icon Glossary and Menu Charts A 1 Touch Screen Icon 1 A 1 A 2 Touch Screen Menu Flow 6 Appendix Replacement Parts Listings vi Foxy R1 and Foxy9 R2 Fraction Collectors Table of Contents List of Figures 1 1 Foxy R1 and Foxy R2 fraction collectors 1 1 1 2 Fraction collector features 1 5 2 1 Adjusting the height of the standard and high flow diverter valves 2 2 2 2 Mam menu oves Hala ak a ac abe potete
6. Appendix A Icon Glossary and Menu Charts A 1 Touch Screen Icon Table A 1 describes the fraction collector display icons Glossary Table A 1 Touch screen display icons Description Select the Configuration Tools icon to configure the collection racks communication settings and injection overlay settings Select the Folder icon to open a screen from which you can view or modify the current selec tion Touch the Diverter Valve Position icons to control the position of the diverter valve When the arrow points to the tube the valve is set to collect fluids in tubes When the arrow points to the waste can the valve is set to route fluids to waste This region of the display reports the current tube position on the top line The bottom line reports the maximum number of tubes held by the rack or a user specified number of tubes see page 3 4 Touch the Next Tube icon to advance the drop for mer to the next tube position Touch the Play icon from the main menu to start operation Touch the Pause icon during operation to sus pend tube advances While paused the Pump Pause output signal is active to disable an exter nal pump Touch the Stop icon to halt operation The drop former will return to the home position Touch the Standby On icon to toggle the fraction collector between these modes Touch the Enter icon to accept the current set tings and return to the previous screen A
7. N CAUTION Cautions identify a potential hazard which if not avoided may result in minor or moderate injury This category can also warn you of unsafe practices or conditions that may cause property damage N WARNING Warnings identify a potentially hazardous condition which if not avoided could result in death or serious injury NDANGER DANGER limited to the most extreme situations to identify an imminent hazard which if not avoided will result in death or serious injury Foxy R1 and Foxy R2 Fraction Collectors Safety Hazard Symbols Warnings and Cautions gt gt The equipment and this manual use symbols used to warn of hazards The symbols are explained below Hazard Symbols The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument s technical reference manual The lightning flash and arrowhead within the triangle is a warning sign alert ing you of dangerous voltage inside the product Symboles de s curit Ce symbole signale l existence d instructions importantes relatives au produit dans ce manuel AN A Ce symbole signale la pr sence d un danger d lectocution Warnungen und Vorsichtshinweise Das Ausrufezeichen in Dreieck ist ein Warnzeichen das Sie darauf aufmerksam macht daB wichtige Anleitungen zu diesem Handbuch geh ren gt gt Advertencias y Precauciones Der gepfeilte Blitz im Drei
8. Touch the Threshold Detection Options icons to select a detection mode The top icon indicates that threshold peak detection is OFF The middle Union icon indicates that threshold peak detec tion is enabled If the value is exceeded the peak state will be true regardless of any other detec tion mode a logical OR The bottom Intersection icon indicates that peak detection is enabled If the value is exceeded the peak state is true only when other enabled detection conditions are met a logical AND Touch the Peak Width Detection icon to configure the peak width settings A border around this icon indicates that peak width detection is enabled When editing the peak width settings touch the icon to toggle between collection modes The left icon indicates that only peak fluids will be col lected non peak fluids are diverted to waste The right icon indicates that peak and nonpeak fluids will be collected In this mode the system uses a user specified peak width value and an algorithm to determine a range of possible input signal slopes A peak width condition is true when the analog input sig nal rises at a rate within this range However the peak state may or may not be true depending on the Peak Width Detection Options A 4 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts Table A 1 Touch screen display icons Continued Icon Description Touch the Peak Width Detection Option
9. R2 Fraction Collectors Section 4 Serial Command Control UPLOAD n This command chooses which program line n 0 to 71 is to be sent next Example Command Response UPLOAD 0 none UPLOAD 1 This command responds with the command strings for the entire current program immediately Example Command Response UPLOAD 1 NOTE PROGRAM 3PTYPE 4 LAST 144PATERN 1 FTYPE 1FSIZE 1 DELAY ORANGE 1 THRESHOLD 0 WIDTH 1 1NONPEAK 3 RESTART 2 WINDOW 1 WSTART 0 WEND 60WINDOWZ 2 WSTART 120 WEND 240 NOTE Config Pump off 0 Only rack 11NOTE Pattern 3 Peak det 5 Motor 5NOTE Cal OmV 32768 1V 58982 NOTE 100mV 58982 10mV 35389 NOTE Time 749 VREF 500 Bklash ONOTE FLLR 128 FLFB 128 FRLR 128NOTE FRFB 128 BLLR 128 BLFB 128NOTE END Unsupported Programming Commands The following serial commands are not supported by the fraction collector ESTATE n ETIME n ETYPE n EVENT n LIST PTYPE These commands were available for earlier fraction collector models Do not use them with the Foxy R1 and R2 fraction col lectors 4 16 4 5 4 Problem Responses F oxy9 and R2 Fraction Collectors Section 4 Serial Command Control The fraction collector will respond with a PROBLEM lt type gt message should the command fail The following types may be reported IMPROPER MODE The program values cannot be changed while a run is in progress M Note RUN and STOP cannot be executed w
10. IL F SCALE 0 625 gt HIGH FLOW FOXY DRAWING NO 602132669B SHEET 9 OF 12 B 33 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix Replacement Parts Listings 602132669 REPLACEMENT PARTS LIST SHEET 10 OF 12 TELEDYNE ISCO HIGH FLOW FOXY R2 REV B T PART NUMBER DESCRIPTION 231014508 8 32 X 1 02 PANHEAD SCREW 2 233211200 8 INTERNAL TOOTH LOCK WASHER B 233011200 8 FLAT WASHER 4 605233016 SHORT BLACK FOO 5 602133633 MAIN BASE BOTTOM COVER 6 231014506 8 32 X 3 8 PANHEAD SCREW 6921341 15 RFID SENSOR RIBBON CABLE 8 692134647 RFID CBA 9 231011210 140 X 19 LONG SPACER 0 233210800 6 INTERNAL TOOTH LOCK WASHER 231014406 6 32 X 3 8 PANHEAD SCREW 2 21535662 BASE LABE 3 52 333296 RFID COVER 4 2133626 DRIP TRAY 100513 6 32 X 3 8 FLH SCREW BLACK CHERRY 6 2135088 CPU CBA 1 341000003 POWER SUPPY 24VDC 8 602133679 CPU CBA FISH PAPER 9 602133691 DROP FORMER VALVE CBA COVER 20 602133610 MAIN ARM TOP COVER 2 231814325 X 25 PANHEAD SCREW 22 233210400 4 INTERNAL TOOTH LOC
11. 5 2 4 Drop Counter Cleaning 5 2 5 WARNING If collecting fluids that can produce flammable vapors perform the Tubing Inspection 5 2 1 and the Rack and Drip Tray Cleaning 5 2 2 at least monthly WARNING Risk of fire or equipment damage Faulty tubing fittings and drains may allow organic solvents to pool in unsafe areas creating a potential for dangerous levels of flammable vapors Improper draining may damage the instrument s internal components If the fraction collector is used with solvents that produce flam mable vapors perform a tubing inspection monthly 1 Visually inspect the diverter valve inlet and waste lines The tubing must be free of any damage kinks or deterio ration Fittings should show no signs of leaks 2 Visually inspect the drip tray drain tubing The tubing should be free of any damage kinks deterioration or obstructions F oxy9 R1 and R2 Fraction Collectors Section 5 Maintenance 5 2 5 2 2 Collection Rack and Drip Tray Cleaning 5 2 3 Exterior Cleaning 5 2 4 Fluid Path Cleaning Correct any deficiencies before returning the instrument to oper ation WARNING Risk of fire or equipment damage Dirty collection racks and drip tray might inhibit their conductive properties The racks and tray must be kept clean to dissipate static electricity Collection tube racks and drip tray should be kept clean so that they dissipate static electricity Dirt
12. Appendix A Icon Glossary and Menu Charts SEM lt gt Y A Y A Figure A 4 Saved method recall and method name editing A 9 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts Foxy R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings Replacement parts are called out in the following pages Refer to the call out in the table to determine the part number for the item Purchase replacement parts by contacting Teledyne Isco s Cus tomer Service department Teledyne Isco Customer Service Department P O Box 82531 Lincoln NE 68501 USA Phone 800 228 4373 402 464 0231 FAX 402 465 3022 E mail IscoInfo teledyne com Outside USA or Canada contact your local representative http www isco com Iccontact B 1 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings ac EN gt lt Le DRAWING NO 603872162C B 2 4X Foxy R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings FOXY RI DRAWING NO 603872162C SHEET 2 OF I B 3 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings DETAIL SCALE 0 500 FOXY RI
13. VALVE 1 Unsupported Immediate Control Commands The following serial commands are not supported by the fraction collector e GRADIENT n OSTATE n e OUTPUT n PSPEED n These commands were available for earlier fraction collector models Do not use them with the fraction collectors DEFAULT 1 This command clears the current program to defaults To minimize accidental clearing the characters 1 are required Example Command DEFAULT 1 Response none DELAY n This command sets the flow delay of the current program If FTYPE 1 fraction by time then n 0 to 359999 seconds If FTYPE 2 or fraction by drops or external counts then n 0 to 999999 counts For example the string FTYPE 1 DELAY 102 sets a flow delay of 1 minute 42 seconds Example Command DELAY 102 Response none The DELAY command without an operand returns the current setting 4 9 Foxy9 R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 10 Example Command DELAY Response DELAY 102 FSIZE n This command sets the fraction size for the current program If FTYPE 1 fraction by time 1 to 359999 seconds if FTYPE 2 or 3 fraction by drops or external counts n 0 to 999999 Example Command FSIZE 23 Response none The FSIZE command without an operand returns the current setting Example Command FSIZE Response FSIZE 23 FTYPE n This command sets the fraction type of the current program Choices
14. crystals oils etc can degrade the performance of the fluid lines as well as the fraction collector s diverter valve and drop counter operation If you experience problems with the fluid path or the diverter valve outlet flush the fluid path with miscible solvent or buffer Difficult obstructions may require stronger solvents or buffers as a cleaning fluid Consider allowing the cleaning fluid to remain in the fluid path overnight F oxy9 R1 and oxy R2 Fraction Collectors Section 5 Maintenance If problems occur frequently consider pumping a cleaning fluid through the path as final step in each collection routine 5 2 5 Drop Counter Cleaning The drop counter is a photosensor device at the outlet of the standard flow rate diverter valve This device uses a glass sleeve to protect the electronic components If the performance of the drop counter degrades the glass sleeve should be cleaned To clean the glass sleeve 1 Place the fraction collector in Standby and remove the AC mains power cord 2 Remove the collection racks 3 Carefully unscrew the black retaining ring located under the diverter valve The glass sleeve rests inside the retain ing ring a Glass sleeve IX a ZE Retaining ring Figure 5 1 Retqining ring for the drop counter s glass sleeve 4 Clean the glass sleeve with a mixture of distilled water and a mild detergent Use isopropyl alcohol for
15. 1 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts Table A 1 Touch screen display icons Continued Icon Description Touch the Rack icon to open a screen from which you can select the rack type and collection pat tern and calibrate the drop former position Wi o ep The Tube Advance Speed icon allows you to access a screen from which you can set the speed of the tube advance motion From the tube advance speed screen touch this icon to speed up the movement Touch the Slow icon to slow the tube change movement This icon indicates the tube advance speed set ting The speed setting ranges from 1 slowest to 5 fastest Touch the Calibrate icon to open a screen from which you can adjust the drop former position When calibrating the arm touch the First Tube Position icon to move the drop former over the first tube The drop former will move to the first tube position for the current rack configuration When calibrating the arm touch the Last Tube Position icon to move the drop former over the last tube The drop former will move to the last tube position for the current rack configuration On two rack fraction collectors the last tube posi tion is on the second rack N The Arrow icons perform several functions In some screens the up and down arrows incre ment and decrement alphanumeric values In other screens the arrows move the cursor posi tio
16. 10 3 3 2 Drop We eatem E a 8 11 3 3 9 Time Intervals 1 1 2 e HRH HH HHHH HHHH HHHH n 8 18 9 3 4 Volume btw tok Um rede ee 3 14 3 8 5 Threshold Detection gt 30043 a09 eee 8 15 3 3 6 Peak Width Detection 3 17 3 8 7 Time Windows 3 20 Foxy9 R1 and Foxy R2 Fraction Collectors Table of Contents Section 4 Serial Command Control 47 Introductiom eve r D ggD ererereerggmmm_ Pbv gZZxu 4 1 4 2 RS 232 Communication 4 1 4 3 Ethernet Communication Information 4 1 4 4 General Command 4 1 4 4 1 Character Format pP vesc cea e py rzIyperY vtr 4 1 4 4 2 Command Syntax noi an da d IR uei a e ZG Ead 4 2 AAS Line tuu uA ee eds es 4 2 4 444 Mode S eR RE eed vam RUN que VE CE ga eite 4 2 4 2 4 416 Timing xel aj I Re S S a 4 2 4 5 Serial Commands 0 sa a a l keni diriye y W ne has 4 2 4 5 1 Miscellaneous Commands 4 2 4 5 2 Immediate Control Commands 4 6 4 5 8 Programming Commands 4 9 4 5 4 Problem Responses
17. 13 Foxy R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 2 14 Foxy R1 and Foxy R2 Fraction Collectors Section 3 Programming This section contains an overview of fraction collection pro gramming via the touch screen interface Programming via the touch screen supports basic collection applications where the fraction collector is one of several discrete components with or without a main controller Control signals if any are connected to the Pump or Detector connectors on the back panel For advanced collection applications where the fraction collector is part of a system directed by an external controller refer to Section 4 Serial Command Control The fraction collector in these applications is interfaced with an external controlling device through the RS 232 Serial or Ethernet connectors as well as the optional pump and detector inputs The display shows a computer icon if the system is under remote control Refer to Section 4 for serial control programming instructions and to the docu mentation supplied with the controlling device Should there be a need to control the fraction collector locally touch the computer icon to terminate the remote control Note The display shows a lock icon if the system is under exclusive remote control Refer to the documentation supplied with the controlling device for programming instructions Additional touch screen programming resources can be found
18. 2133618 INTERNAL VALVE COVER 4 2133623 VALVE ADJUSTMENT CLAMP 4 2133619 VALVE COVER 43 2133658 DROP FORMER SLEEVE RETAINER 44 93813015 DROP COUNTER SLEEVE 45 02133621 DROP FORMER 46 209016319 FERRULE W RING FOR 1 8 OD 4 602134412 KNOB ASSEMBLY 48 231514310 4 40 X 5 8 PANHEAD SCREW 49 692134649 DROP FORMER MOTOR ENCODER CBA 50 231012000 140 X 31 LONG SPACER NOTE For current prices and quotations on parts contact Isco Service Department 2 This list is subject to change without notice B 22 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings REPLACEMENT PARTS LIST TELEDYNE ISCO 602132662 SHEET 11 OF 2 FOXY R2 REV PART NUMBER DESCRIPTION 26 231014304 1 4 PANHEAD SCREW 21 233100400 WASHER 28 69523412 ARM OPTICAL SWITCH 29 20900330 EAR RAIL amp SLIDER 30 602133670 ARM IDLER PULLE 3 209003033 ARM DRIVE BEL 32 602133616 ARM SENSOR FLAG 33 231116306 CAP SCREW 34 233010403
19. 4 FLAT WASHER 60 602135086 DISPLAY RIBBON CABLE 6 602135085 CPU CBA TO CO CBA RIBBON CABLE 2 692134164 ARM OPTICAL SWITCH 3 231514306 4 40 X 3 8 PANHEAD SCREW 4 602133666 ARM IDLER PULLEY 5 602134413 DISPLAY W BE ASSEMBLY 6 602134411 ARM DRIVE BELT W SPLICE 489000001 CABLE CLIP W ADH BACK NOT SHOWN 68 029071404 5 16 X 1 16 VINYL TBG NOT SHOWN 69 023050204 03 ID X 062 OD TEF IBG NOT SHOWN 10 602134115 ADJUSTABLE FOOT ASSEMBLY r 231 5143068 4 40 X 1 02 PAN HEAD SCREW N uu ci p Mu tu E B 24 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix Replacement Parts Listings HIGH FLOW FOXY R2 x 602132669B SHEET OF 12 B 25 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings HIGH FLOW FOXY R2 DRAWING NO 602132669B SHEET 2 OF 12 B 26 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix Replacement Parts Listings HIGH FLOW FOXY R2 DRAWING Ni 602132669B SHEET 3 OF I2 B 27 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings HIGH FLOW FOXY R DRAWING NO 6021326698 SHEET 4 OF 12 B 28 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix Re
20. 8 cm Foxy R2 High Flow PN 68 2130 007 12 25 21 0 15 5 in 31 1 53 3 39 4 cm Weight with drip tray no tube rack Foxy R1 15 6 Ibs 7 1 kg Foxy R2 22 8 Ibs 10 3 kg Foxy R2 High Flow 22 9 1 5 10 4 kg Available Racks 12 mm diameter tubes 144 rack 13 mm diameter tubes 144 rack 16 mm diameter tubes 100 rack 18 mm diameter tubes 72 rack 25 mm diameter tubes 36 rack 1 5 mL microcentrifuge tubes 60 rack 50 mL centrifuge tubes 36 rack 28 mm diameter scintillation vials 36 rack 96 well microplates R1 rack holds 2 plates R2 rack holds 6 plates MiniVials 72 rack 480 mL bottles Funnel racks for fractions larger than 480 mL Maximum Tube Height Foxy R1 160 mm Foxy R2 196 mm Foxy R2 High Flow 180 mm Maximum Flow Rate Foxy R1 68 3870 010 Standard flow rate of 25 mL min through 1 16 inch I D tubing Optional accessory package 60 2139 032 extends maximum flow rate to 125 mL min through e inch I D tubing Foxy R2 68 2130 006 Standard maximum flow rate of 125 mL min through 1 8 inch I D tubing Optional accessory package 60 2139 031 reduces flow rate to 25 mL min through 1 16 inch I D tubing Foxy R2 with high flow diverter valve 68 2130 007 1000 mL min Delay Volume 1 6 Drop former located beneath diverter valve for uL delay volume Compensation only required for length of tubing to the diverter valve Foxy9 R1
21. ER 9 602133620 G BACK COVER 36 209016324 ESS NUT FOR 1 16 OD TBC od 209016321 RING FOR 1 16 OD TUBING 38 2133629 Y WITH 1 4 28 PORTS 39 2134416 ER amp VALVE JCI CBA ASSY 40 2133618 VE COVER 4 2133623 USTMENT CLAMP 4 2133619 43 2133658 ER SLEEVE RETAINER 44 93813015 SLEEVE 45 0213362 46 209016319 RING FOR 1 8 OD A 60213441 BLY 48 231514310 PANHEAD SCREW 49 692134649 MOTOR ENCODER CBA 50 231012000 LONG SPACER NOTE For current prices and quotations on parts contact Isco Service Department This list is subject to change without notice B 23 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix Replacement Parts Listings 502132662 REPLACEMENT PARTS LIST SHEET 12 OF 12 TELEDYNE ISCO FOXY R2 REV C PART NUMBER DESCRIPTION 5 60213447 DROP FORMER MOTOR ASSEMBLY 92 602134414 DROP FORMER MOTOR W ENCODER ASSY 53 232910100 4 40 LOCKING HEX NUT 54 602133607 ARM SENSOR FLAG 55 692134643 CONNECTOR CBA 56 602134475 A ARM MOTOR W ENCODER CBA ASSY a 602134410 ARM MOTOR ASSEMBLY 58 692134654 ARM MOTOR ENCODER CBA 59 233010400
22. Enter the Flow Delay volume as 1 to 999 counts using the arrow icons The Up and Down Arrows increment or decre ment the numbers the Left and Right Arrows move the cursor position To calculate the number of pump counts for the delay vol ume Prounts 1 4 x D x L Bog otherwise expressed as Peounts 0 7854 x D x L Where L is the tubing length and D is the inside diameter is the volume delivered for each pump count Round to the nearest whole number M Note For the greatest possible flow delay volume accuracy cut the tubing so that it holds a multiple of the of the pump count vol ume 6 Touch the Enter icon to return to the Methods Settings menu 3 3 5 Threshold Detection Threshold detection isolates the most concentrated portion of peaks The threshold is set as a percentage of the maximum input signal When the input signal rises above this threshold SS OT 2 value the fraction collector performs a tube advance to isolate the peak An example of threshold peak detection and collection is shown in Figure 3 13 The peaks on the analog signal are numbered 1 through 4 The fraction collector ignores the first peak because it is below the 10 threshold It isolates peaks 2 through 4 in col lection tubes because the peak signal exceeds the threshold Percent Time Isolated Peaks grey Figure 3 13 Threshold detection cuts peaks when external peak signal exceeds set percen
23. Waste NO IN Common LH Drop Former NC Figure 2 9 High flow diverter valve ports front view N CAUTION Risk of fire or equipment damage caused by electrostatic dis charge Organic solvents delivered at high flow rates can build an electrostatic charge The conductive black tubing supplied in the kit dissipates the electrostatic charge If handling organic solvents at high flow rates do not substitute the conductive tubing Note Cut the tubing with a tubing cutter to ensure that the end is cut cleanly and free from any deformation 4 Prepare one end of each tubing piece as shown in Figure 2 10 Valve Fitting Back Ferrule Front Ferrule Figure 2 10 Tubing preparation high flow 5 Using two wrenches connect the In and Waste tubing to the valve using caution to prevent damage to the plastic valve body 2 8 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use N CAUTION Plastic valve body damage possible While swaging the tubing connections always use two wrenches to prevent turning the metal fittings in the plastic valve body Use one wrench to hold the inside nut to keep it from turning Use the second wrench to turn the outer nut 6 Connect the other end of each tubing piece to their respec tive source or waste container 7 Ensure that
24. advances based on a variable input signal The fraction collector reads this input signal from an external device to isolate peak fluids from non peak fluids Typically the input signal represents UV light absorbing compounds in chromatographic eluate Signals may also represent other measurable liquid properties such as visible light absorbance fluorescence pH or any parameter with an analog output signal Tube advances at the leading and lagging edges of the peak isolate peak fluids from non peak fluids com monly called peak cutting These peak collection modes are Threshold Peak Detection The fraction collector can per form a tube advance whenever the monitored signal passes a user specified threshold The threshold set as a percent age of the input signal typically is set high enough to elim inate false peaks due to a noisy signal baseline Peak Width Detection The fraction collector can perform a tube advance when the rising or falling slope of the input signal indicates the leading or lagging edge of a peak The user selected peak width setting is part of a peak detection algorithm which differentiates true peaks from false indi cations such as noise and shifting baselines With the cor rect peak width setting this collection mode can cut closely eluting and even overlapping peaks Threshold and peak width detection may be combined together Additionally whether one or both peak detections modes are used the fract
25. and Foxy R2 Fraction Collectors Section 1 Introduction Table 1 2 Specifications Continued Standard Diverter Valve Internal Volume Drop former NC 110 uL Waste NO 130 uL Diverter Valve Inlet Pressure 25 psi 1 723 bar maximum standard and high flow valves Peak Detection Threshold level peak width slope detection time windows System Control Operator Front panel control via touch screen LCD Integrated systems Direct communication via Ethernet TCP IP and RS 232 serial communications Stored Methods Eight Operating Voltage 100 240 VAC 50 60 Hz 1 ampere maximum Mains voltage must not exceed 10 of the nomi nal supply voltage Operating 0 to 40 C Temperature 32 to 104 F Humidity 95 relative humidity maximum Internal heaters protect internal components Inputs Pump counter external peak inject tube advance analog peak voltage Outputs Pump pause tube change event Wetted Materials Valve PEEK and Perfluoroelastomer FFKM Supplied ferrules ETFE Supplied valve tubing PTFE Supplied drain tubing Vinyl Typical Applications Prep HPLC peptide protein purification soil extraction density gradient fractionation Electrical Safety per EN 61010 1 Second Edition Maximum Altitude 2000 meters Pollution Degree Installation Category 2 II a Teledyne Isco is continually improving its pro
26. arp Gee c eet poca dun 2 3 2 3 Method Select screen 2 3 2 4 Edit Method Settings screen 2 4 2 5 Time based Fractions screen 2 4 2 6 Diverter valve ports s ro scere nai eny al w da de ue a en 2 5 2 7 Tubing preparation tiea E Wla ee rn 2 6 2 8 Connections to standard diverter valve 2 7 2 9 High flow diverter valve ports front 2 8 2 10 Tubing preparation high 2 8 2 11 Connections to high flow diverter valve 2 9 2 12 Rack guide pins and bottle location ridges 2 11 2 13 Rack installed with ground post touching guide pin 2 11 2 14 Diverter Valve adjusted with 1 9 inch or 1 em 2 12 o51 Main Menu s ERES eme m EE EE ERE V de 3 2 3 2 Configuration Tools Menu 3 3 3 3 Rack options menu sois sere se Seau eral r kk rr rn 8 8 3 4 Inject options timing diagram 3 7 3 5 Timed options timing diagram 3 7 3 6 Overlay Next and Skip options diagram 3 8 3 7 Method Settings
27. eer ei hr 2 1 Fluid Connections REIR LA Dk SEY Ro RW y A A d EU IUE 2 5 2 2 1 Standard Diverter Valve Connections 2 5 2 2 2 High Flow Diverter Valve Connections 2 7 2 2 8 Drip Tray Drain Connection 2 9 Rack Installation dan e n ag t eee d ale Qars gl de 2 10 Diverter Valve 2 12 Installation Qualification 2 13 Section 3 Programming 3 1 Mam Menu sern i Iluangq ecce WEDA d epa V puce ee ics 3 2 3 2 Configuration 3 3 9 21 Rack Settings os ERR ol SEO K k Ay den E Seach oe ee ees 3 3 3 2 2 RS 232 Serial 3 5 8 29 TCP IP Settings 43 5 2s KeA IE LG Wu LE Aka TE Skee eS 3 6 3 2 4 Inject Options s socre xwee kan cee rr rn 3 6 3 2 5 Pump Control Settings 3 9 3 2 6 Analog Peak Settings ss safe eee er l lk 3 9 3 2 7 Fraction Collector Information 3 9 3 3 Method Settings ccs LA ERR Geechee ein kr dI geh ed 3 10 3 3 1 Method Selection 3 3 cema Gor sha air a ase hack 8
28. icon Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts Table A 1 Touch screen display icons Continued Icon Description Touch the Volume icon to collect fractions based on an external pump volume count Touching this icon opens a screen from which you can set the fraction and delay volumes as a number of pump counts If selected the display shows a border around this icon Each touch of the Volume Select icon toggles between volume setting modes The current selection is indicated by the surrounding border When the hourglass left is selected enter the delay volume When the tube right is selected enter the fraction size The units for each setting reflect the selected Time Drop or Volume frac tion collection mode Touch the Threshold Peak Detection icon to con figure the threshold settings A border around this icon indicates that threshold peak detection is enabled When editing the threshold settings touch this icon to toggle between collection modes The left icon indicates that only peak flu ids will be collected non peak fluids are diverted to waste The right icon indicates that peak and nonpeak fluids will be collected Threshold detection monitors the percentage of an analog input signal The threshold condition is true when the input signal exceeds a user set value However the peak state may or may not be true depending on the Threshold Detection Options
29. n The maximum number of windows is four This command sets which window number 1 will be used for the WSTART and WEND commands that follow Example Command WINDOW 1 Response none F oxy9 and R2 Fraction Collectors Section 4 Serial Command Control The WINDOW command without an operand returns the current setting Example Command WINDOW Response WINDOW 1 WSTART n This command sets the window start time n 0 to 359999 seconds of the currently selected window Example Command WSTART 36000 Response none The WSTART command without an operand returns the current setting Example Command WSTART Response WSTART 240 WEND n This command sets the window end time n 0 to 359999 seconds of the currently selected window Example Command WEND 60 Response none The WEND command without an operand returns the current setting Example Command WEND Response WEND 240 UPLOAD The unit responds to this command with the current program settings one line at a time The UPLOAD command must be sent for each line in the program until NOTE END is received from the unit Settings are in the form of commands allowing them to be sent back to the unit to restore a program All settings are sent even if they are not relevant to the way the program is currently set Example Command UPLOAD Response PROGRAM 1 Command UPLOAD Response DELAY 0 4 15 F oxy9 R1 and
30. or decre ment the numbers the Left and Right Arrows move the cursor position 4 Touch the Volume Select icon to display the Flow Delay vol ume The hourglass should be highlighted with a border Zl 5 Enter the Flow Delay volume as hours minutes and sec onds using the Arrow icons The Up and Down Arrows increment or decrement the numbers the Left and Right Arrows move the cursor position 6 Touch the Enter icon to return to the Methods Settings menu About Flow Delays A Flow Delay synchronizes the tube changes with sample flow to make precise peak cutting possible The flow delay time is the time required for a fraction to travel from your detector to the fraction collector s drop former The delay depends on the flow rate and the size of the tubing between the fraction collector and the detector To calculate the flow delay time use this formula T2V Q Where T is the delay time in seconds V is the internal volume of the tubing and Q is the flow rate in the same units as V per second 3 12 The volume of the tubing can be calculated by V 2n AxD xL orexpressed as V 0 7854 x D x L where L is the tubing length cm and D is the inside diameter cm The resulting volume is in milliliters An easy alternative way to find the delay time is to introduce a bubble ahead of the detector and measure the delay to the drop former 3 3 3 Time Intervals 3 Figure 3 11 Time Intervals displ
31. setting 2 A 4 Time Isolated Peaks grey Percent Figure 3 15 Peak Width detection cuts peaks when internal slope algorithms indicate a peak To program the fraction collector for Peak Width detection 1 Touch the Peak Width Detection icon from the Method Set tings menu to open the threshold menu Figure 3 16 3 17 Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming Figure 3 16 Peak Width Settings menu 3 18 2 Enter the average peak width using the Up and Down Arrow icons The Up and Down Arrows scroll through the available Peak Width settings Enter the average time duration of the expected peak as measured at the baseline The fraction collector will use this average time to detect peaks with widths ranging from 2 to 2 times the programmed width For best results choose the next larger peak width if your expected peak width falls between the available options For example If you expect a peak of 45 seconds enter an average peak width of one minute The fraction collector will detect peaks with widths ranging from 9 seconds to 2 minutes The icon next to the percentage shows what the fraction collector will do with non peak fluids The icon that is clear below the threshold indicates that non peak fluids will be diverted to waste The icon that is shaded below the threshold indicates that non peak fluids will be collected in tubes Touch the icon to toggle the sel
32. the current tube Touch the Stop icon The arm and drop former will move to the home position This completes the preliminary checkout procedure Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 2 2 Fluid Connections There are three points for fluid connections on the fraction col lector Two are on the diverter valve In and Waste The third point is found on the drip tray which you can route to a waste container N CAUTION Risk of fire or explosion Nonconductive nonpolar liquids at lin ear velocities greater than 40 cm sec may develop an electro static charge If this applies to your application use conductive tubing and ground the fluid path Refer to section 2 2 1 for instructions on connecting tubing to the standard diverter valve Refer to section 2 2 2 for instructions on the high flow rate valve connections Both valves are shown in Figure 2 1 on page 2 2 2 2 1 Standard Diverter Valve Connections M Note The In and Waste connections use special headless fittings The position of the ports prevent the use of standard Upchurch fittings Figure 2 6 shows the standard diverter valve ports IN Waste Common NO So e o _ Drop Former NC Figure 2 6 Diverter valve ports To connect the fluid lines 1 Place the unit in Standby by touching the Standby ON icon 2 Remove any installed racks 3 Loosen the diverter valve thumb screw and lower the div
33. to change product specifications replacement parts schematics and instruc tions without notice Contact Information Customer Service Phone 800 228 4373 USA Canada Mexico 402 464 0281 Outside North America Fax 402 465 3022 Email IscoCSR teledyne com Technical Support Phone 800 775 2965 Analytical 866 298 6174 Samplers and Flow Meters Email IscoService teledyne com Return equipment to 4700 Superior Street Lincoln NE 68504 1398 Other Correspondence Mail to P O Box 82531 Lincoln NE 68501 2531 Email IscoInfo teledyne com Revised March 17 2009 Foxy R1 and Foxy R2 Fraction Collectors Table of Contents Section 1 Introduction 11 1 2 1 8 1 4 1 5 1 6 Collection Schemes 23 oi eR ea ee eer em dp areis 1 2 Racks and Vessels 2 5 KOR bb 1 8 FeabL fres ev e S E D EE AREE NS 1 4 Technical 1 6 Rear Panel Connectors 5 ss v xWe A A0 hn 1 8 Accessory Cable 1 10 1 6 1 Cables for Isco Accessories 1 10 1 6 2 Cable for non Isco Pumps 1 10 1 6 3 Cable for non Isco Detectors 1 11 Section 2 Preparation for Use 2 1 2 2 2 8 2 4 2 5 Preliminary Checkout
34. 30 days of shipment Throughout the remainder of the warranty period the customer will pay to return the instrument to Teledyne Isco and Teledyne Isco will pay surface transportation to return the repaired instrument to the customer Teledyne Isco will not pay air freight or customer s packing and crating charges This warranty does not cover loss damage or defects resulting from transportation between the customer s facility and the repair facility Teledyne Isco limited warranty The warranty for any instrument is the one in effect on date of shipment The warranty period begins on the shipping date unless Teledyne Isco agrees in writing to a different date Excluded from this warranty are normal wear expendable items such as charts ribbon lamps tubing and glassware fittings and wetted parts of valves and damage due to corrosion misuse accident or lack of proper maintenance This warranty does not cover products not sold under the Teledyne Isco trademark or for which any other warranty is specifically stated No item may be returned for warranty service without a return authorization number issued by Teledyne Isco This warranty is expressly in lieu of all other warranties and obligations and Teledyne Isco specifically disclaims any warranty of merchantability or fitness for a particular purpose The warrantor is Teledyne Isco 4700 Superior Lincoln NE 68504 U S A This warranty applies to the USA and countries wher
35. 306 4 40 X 3 8 PANHEAD SCREW 42 692134164 AIN ARM OPTICAL SWITCH 43 602135085 CPU CBA TO CONN CBA RIBBON CABLE 44 60213508 DISPLAY RIBBON CABLE 45 60213508 DROP FORMER 46 23301040 FLAT WASHER 41 60213448 THUMB SCREW ASSEMBLY 6 32 X 1 2 48 23151431 X 5 8 PANHEAD SCREW 49 69213464 DROP FORMER MOIOR ENCODER CBA 50 23101200 140 X 31 LONG SPACER NOTE 1 For current prices and quotations on parts contact Isco Service Department 2 This list is subject to change without notice B 35 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix Replacement Parts Listings TELEDYNE 15 0 REPLACEMENT PARTS LIST HIGH FLOW FOXY R 602132669 SHEET 12 OF REV 2 a PART NUMBER DESCRIPTION 5 60213441 DROP FORMER MOTOR ASSEMBLY 5 602134414 DROP FORMER MOTOR W ENCODER CBA ASSY 53 232910100 4 40 LOCKING HEX NUT 54 2133607 A ARM SENSOR FLAG 59 2134643 CONNECTOR 56 2134415 AR OTOR W ENCODER ASSY 2d 213441 AR OTOR ASSEMBLY 58 213465 OTOR ENCODER CBA 59 209016368 FRONT FERRU 1 4 OD BING 60 20901636 BACK FERRULE 1 4 OD TUBING 6 20901655 ALE TUBING CONNECTOR 1 4 OD IBG 6 602133694 INLET WASTE TUBE 42 LG NOT SHOWN 63 480654503 CAT
36. 5 CABLE 3 LONG NOT SHOWN 64 02305031 188 ID X 25 OD IBG NOT SHOWN 65 029071404 5 16 X 1 16 VINYL IBG NOT SHOWN 66 231514308 4 40 X 1 2 PANHEAD SCREW NOTE 2 For current prices and quotations on parts contact Isco Service Department This list is subject to change without notice B 36 Foxy R1 and Foxy R2 Fraction Collectors Index A AC power cord 2 2 addresses IP and MAC 3 6 AND combined peak detection 3 16 3 18 3 19 applications typical 1 7 c cables CATS 4 1 Isco detector 1 10 Isco pump 1 10 non Isco detector 1 11 non Isco pump 1 10 calibration drop former position 3 5 5 cable 4 1 checklist installation qualification IQ 2 13 claims damage 2 1 5 4 cleaning drip tray and racks 5 2 drop counter 5 3 exterior 5 2 fluid path 5 2 collection rack cleaning 5 2 grounding 2 11 installation 2 10 part numbers 1 3 collection tube pattern 3 4 collection tube volumes by drop counts 3 12 by pump counts 3 14 by time intervals 3 13 commands serial immediate control 4 6 miscellaneous 4 2 problem responses 4 17 programming 4 9 syntax 4 2 communications ethernet 4 1 RS232 serial 4 1 configuration settings 3 3 connections diverter valve 2 5 2 7 drip tray drain tubing 2 9 electrical 1 8 to 1 11 fluid 2 5 connectors AC mains power 1 8 detector 1 9 ethernet 1 8 pump 1 9 rear panel
37. 5k salla a n k da eee enn has 1 8 1 4 Ethernet Connector Pins 1 8 1 5 Pump Connector PINS sw sk l ka t k k A yan dy eere 1 9 1 6 Detector Connector Pins 1 9 1 7 RS 232 Serial Communication Connector Pins 1 10 1 8 Non Isco Pump Cable P N 69 2134 170 1 11 1 9 Non Isco Detector Cable P N 69 2184 171 1 11 5 1 Installation Qualification Checklist 2 13 1 Touch screen display icons A 1 viii General Warnings Foxy9 R1 and Foxy9 R2 Fraction Collectors Safety Before installing operating or maintaining this equipment it is imperative that all hazards and preventive measures are fully understood While specific hazards may vary according to location and application take heed of the following general warnings WARNING Liquids associated with this instrument may be classified as carcinogenic biohazard flammable or radioactive Shouldthese liquids be used itis highly recommended that this application be accomplished in an isolated environment designed for these types of materials in accordance with federal state and local regulatory laws and in compliance with your company s chemical hygiene plan in the event of a spill N WARNING Avoid hazardo
38. DRAWING NO 603812162C SHEET 3 OF 11 B 4 9 Will 30 Luvd Foxy R1 and Foxy9 R2 Fraction Collectors Appendix B Replacement Parts Listings FOXY 5038721626 5 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings FOXY RI DRAWING NO 603872162C SHEET 5 OF 11 B 6 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix B Replacement Parts Listings 7 63 2x DETAIL B SCALE 0 625 SEE DETAIL DETAIL SEE DETAIL SCALE 0 625 FOXY RI DRAWING NO 603812162C SHEET 6 OF II B 7 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings DETAIL D SCALE 0 750 FOXY DRAWING NO 6038721 62C SHEET 7 OF II B 8 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix B Replacement Parts Listings FOXY DRAWING Ni 603872162C SHEET 8 OF I B 9 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings
39. ET 8 OF 12 B 20 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix B Replacement Parts Listings DETAIL F SCALE 0 600 DETAIL E SCALE 0 600 FOXY R2 602 398620 SHEET 9 OF 12 B 21 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings 502132662 REPLACEMENT PARTS LIST SHEET 11 OF 12 TELEDYNE ISCO FOXY R2 REV ae PART NUMBER DESCRIPTION 26 231014304 4 40 X 1 4 PANHEAD SCREW 2 233100400 SPLIT LOCK WASHER 28 6950234102 DROP FORMER ARM OPTICAL SWITCH 29 20900330 AIN AR INEAR RAIL amp SLIDER 30 602133670 DROP FORMER ARM IDLER PULLEY 3 209003033 DROP FORMER ARM DRIVE BELI 32 602133616 DROP FORMER ARM SENSOR FLAG 23 231116306 4 40 X 5 16 SCREW 34 233010403 4 FLAT WASHER 2 602133620 VALVE HOUSING BACK COVER 36 209016324 4 28 HEADLESS NUT FOR 1 16 OD IBG EN 209016321 FERRULE W Z RING FOR 1 16 OD TUBING 38 2133629 VALVE 3 WAY WITH 1 4 28 PORTS 39 2134416 DROP COUNTER amp VALVE JCI CBA ASSY 40
40. Foxy R1 and Foxy R2 Fraction Collectors i Foxy R2 2 TELEDYNE ISCO Everywhereyoulook Part 69 2133 667 of Assembly 60 2133 665 Copyright 2008 All rights reserved Teledyne Isco Revision G August 20 2012 Foreword This instruction manual is designed to help you gain a thorough understanding of the operation of the equipment Teledyne Isco recommends that you read this manual completely before placing the equipment in service Although Teledyne Isco designs reliability into all equipment there is always the possi bility of a malfunction This manual may help in diagnosing and repairing the malfunc tion If the problem persists call or e mail the Teledyne Isco Technical Service Department for assistance Simple difficulties can often be diagnosed over the phone If it is necessary to return the equipment to the factory for service please follow the shipping instructions provided by the Customer Service Department including the use of the Return Authorization Number specified Be sure to include a note deseribing the malfunction This will aid in the prompt repair and return of the equipment Teledyne Isco welcomes suggestions that would improve the information presented in this manual or enhance the operation of the equipment itself Teledyne Isco is continually improving its products and reserves the right
41. K WASHER 23 602133606 DROP FORMER AR OUNTING BLOC 24 602135090 DROP FORMER VALVE RIBBON CABLE 29 209003302 DROP FORMER AR INEAR RAIL amp SLIDER MS ea dee ER e B 34 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings REPLACEMENT PARTS LIST TELEDYNE 15 0 602132669 SHEET II OF HIGH FLOW FOXY R REV 2 Td PART NUMBER DESCRIPTION 26 231014304 4 40 X 4 PANHEAD SCREW 2 233100400 SPLIT LOCK WASHER 28 695234121 DROP FORMER ARM OPTICAL SWITCH 29 20900330 AIN AR INEAR RAIL amp SLIDER 30 602133670 DROP FORMER ARM IDLER PULLEY 3 209003033 DROP FORMER ARM DRIVE BELT Jl 602133616 DROP FORMER ARM SENSOR FLAG 33 231116306 4 40 X 5 16 CAP SCREW 34 233010403 4 FLAT WASHER 35 EASES ADJUSTABLE FOOT ASSEMBLY 36 2134411 MAIN ARM DRIVE BELT W SPLICE 31 2134413 DISPLAY W BE ASSEMBLY 38 2134419 VALVE ASSY W TBG CONN amp DROP FORMER 39 2134645 VALVE JUNCTION CBA ASSEMBLY 40 2133666 AIN ARM IDLER PULLEY 4 231514
42. LabVIEW 4 1 level adjustment foot 1 4 local mode 3 1 4 2 4 4 logical operators AND OR 3 16 3 18 3 19 lubrication not required 5 1 M main menu 2 2 3 2 mains power cord 2 2 materials wetted 1 7 menus configuration 3 3 drop counting 3 11 main 2 2 3 2 method editing 2 4 method selection 3 10 method settings 3 10 rack settings 3 3 renaming a method 3 11 threshold peak detection 3 16 time intervals 3 13 time windows 3 21 time based fractions 2 4 volume pump count settings 3 14 method selection menu 2 3 3 10 settings 3 10 mode computer icon 3 1 local 4 4 remote 4 2 4 4 N next tube option injection or timed restarts 3 9 O OR combined peak detection 3 16 3 18 3 19 output signals connector pins 1 8 to 1 11 pump control setting 3 9 specifications 1 7 overlay tubes option injection or timed restarts 3 8 P packing 5 4 parts replacement B 1 pattern collection tube 3 4 peak and waste fluids 3 16 3 18 peak detection combining threshold and peak width 3 16 3 18 3 19 external detector 3 9 peak width slope 3 17 threshold 3 15 peak input signal specifications 1 2 peak width slope detection general 1 2 ranges 3 18 settings 3 17 physical dimensions 1 6 power cord 2 2 preliminary checkout 2 1 pump cable Isco 1 10 non Isco 1 10 rear panel connector 1 9 pump control output settings 3 9 pump count settings 3 14 rack cleaning 5 2 conf
43. Volume j Figure 3 12 Volume display 3 14 The Volume option creates fractions by advancing the drop former at a fixed number of pump counts This option relies on a pump count signal delivered by an external device to pin 1 of the fraction collector s Pump connector M Note The Volume option can create relatively uniform collection tube volumes even when the delivered flow rate varies Refer to the documentation provided by the pump manufacturer to determine the volume delivered for each pump count To create fractions based using the volume option 1 Touch the Volume icon to open the settings display Figure 3 12 Use this display to set the fraction volume and delay volume both expressed as pump counts 2 Ensure that the fraction volume setting is active as indi cated by the Volume Select icon That is the tube should be highlighted with a border If not touch the icon to toggle the setting i 3 Enter the fraction volume as 1 to 999 counts using the arrow icons The Up and Down Arrows increment or decre ment the numbers the Left and Right Arrows move the cursor position For example if the pump delivers 2 mL each pump count and the fraction size should be 24 mL enter 12 pump counts 4 Touch the Volume Select icon to display the Flow Delay The hourglass should be highlighted with a border Zi Foxy9 R1 and Foxy R2 Fraction Collectors Section Programming 5
44. ack If a rack is not detected the command will respond with RnDETECT 0 Example Command R1DETECT Response 006500100075 If a rack is detected then the command will typically respond within 500 milliseconds If a rack is not detected then the command will timeout and typically respond within 700 milli seconds STOP This command pauses or stops a run Enter the command once to pause a run in progress Enter the command twice to completely stop the run This command is ignored if the unit is not in a col lection run Example Command STOP Response none TUBE n This command sets the current tube number to n then moves the drop former to it if the drop former is not at home The n operand can be from 1 up to the maximum for the currently pro grammed rack type Example Command TUBE 68 Response none TUBE without an operand requests the tube number the drop former is currently over Example Command TUBE Response TUBE 45 4 5 3 Programming Commands F oxy9 R1 and R2 Fraction Collectors Section 4 Serial Command Control VALVE n This command turns the valve on or off Setting VALVE 0 will cause the flow to the drop former to be diverted to waste with a three way valve Setting VALVE 1 will allow flow to the drop former Example Command VALVE 1 Response none The VALVE command without an operand returns the current valve state Example Command VALVE Response
45. ance speed 3 4 tube height maximum 1 6 tube volumes by drop counts 3 12 by pump counts 3 14 by time intervals 3 13 tube set maximum 3 4 tubing inspection 5 1 Index 3 Foxy R1 Foxy R2 Fraction Collectors Index Index 4 DECLARATION OF CONFORMITY ISM1 A Application of Council Directive 2004 108 The EMC Directive 2002 96 EC The WEEE Directive 73 23 EEC The Low Voltage Directive Manufacturers Name Teledyne Isco Inc Manufacturer s Address 4700 Superior Lincoln Nebraska 68504 1398 USA P O Box 82531 Lincoln NE 68501 2531 Phone 1 402 464 0231 Facsimile 1 402 465 3799 Equipment Type Environment Laboratory Equipment for Light Industrial Commercial Environments Trade Name Model No Foxy R1 R2 Year of Issue 2008 Standards to which Conformity is Declared 61010 1 2 edition Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use EN 61326 1 2003 EMC Requirements for Electrical Equipment for Measurement Control and Laboratory Use Standard Description Severity Applied Performance Criteria EN61000 4 2 Electrostatic Discharge Level 2 4kV contact discharge A Level 3 8kV air discharge EN61000 4 3 Radiated RF Immunity 80 MHz to 1000MHz 80 AM at 1kHz A Level 2 3V m EN61000 4 4 Electrical Fast Transient Level 2 1kV on AC lines A EN61000 4 5 Surge on AC Lines Level 2 1kV common mode A Level 2 0 5KV differential mod
46. are Fraction by Time FTYPE 1 Fraction by Drops FTYPE 2 or Fraction by External counts FTYPE 3 Example Command FTYPE 3 Response none The FTYPE command without an operand returns the current setting Example Command FTYPE Response FTYPE 3 LAST n This command sets the last tube of the current program Choices are n 1 to n maximum number of tubes for programmed rack type Example Command LAST 10 Response none The LAST command without an operand returns the current setting Example Command LAST Response LAST 10 F oxy9 R2 Fraction Collectors Section 4 Serial Command Control NONPEAK n This command sets the non peak window action of the current program Choices are Collect n 1 Divert 2 or Drain 3 Example Command NONPEAK 3 Response none The NONPEAK command without an operand returns the current setting Example Command NONPEAK Response NONPEAK 3 PATTERN n This command sets the rack filling pattern of the current program Choices are Standard n 1 or Left to right only n 2 Example Command PATTERN 1 Response none The PATTERN command without an operand returns the current setting Example Command PATTERN Response PATTERN 1 PROGRAM n This command sets the current program number Choices are 1 to 8 Example Command PROGRAM 8 Response none The PROGRAM command without an operand returns the current setting Examp
47. ay Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming Time intervals create fractions by advancing the drop former at a fixed time intervals This option uses an internal clock M Note Time intervals can create widely varying collection tube vol umes if the delivered flow rate is variable To create fractions based on time intervals 1 Touch the Time Intervals icon to open the settings display Figure 3 11 I lt gt Y A Use this display to set the fraction volume and delay volume both expressed as hours minutes and seconds 2 Ensure that the fraction volume setting is active as indi cated by the Volume Select icon That is the tube should be highlighted with a border If not touch the icon to toggle the setting ii 3 Enter the fraction volume as hours minutes and seconds using the arrow icons The Up and Down Arrows incre ment or decrement the numbers the Left and Right Arrows move the cursor position 4 Touch the Volume Select icon to display the Flow Delay The hourglass should be highlighted with a border Zi 5 Enter the Flow Delay volume as hours minutes and sec onds using the Arrow icons The Up and Down Arrows increment or decrement the numbers the Left and Right Arrows move the cursor position 6 Touch the Enter icon to return to the Methods Settings menu 3 13 Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming 3 3 4
48. collection vessel capacities the factory offers funnel racks These funnel racks direct the fluids to con tainers of any size below the fraction collector The construction materials are listed with each type 60 2137 161 Foxy Preparative Rack with 26 ETFE or ECTFE funnels and 50 ft of 3 8 inch ID vinyl tubing For two rack frac tion collectors only 60 2137 162 Foxy Preparative Rack with 26 ETFE or ECTFE funnels and 50 ft of 3 8 inch FEP lined tubing For two rack fraction collectors only 60 2137 163 Foxy Preparative Rack with 36 ETFE or ECTFE funnels and 50 ft of 1 4 inch ID of vinyl tubing For one and two rack fraction collectors Note All racks and funnels include RFID tags for automatic detection by the Foxy R2 fraction collector Figure 1 2 and Table 1 1 identify key features on the Foxy R1 and Foxy R2 fraction collectors Table 1 1 Fraction collector features 1 The touch screen LCD displays icons for turning the fraction collec tor ON or to Standby configuring basic settings and programming the unit for operation Under program control the diverter valve assembly either depos its fluids into the vessel or out to a user supplied waste container The standard diverter valve assembly includes the drop for mer counter The height adjustment knob allows the user to set drop for mer counter outlet at safe yet minimal distance above the collection tubes or funnels T
49. d does not fit your AC mains power source purchase a connec tor adapter or IEC320C13 power cord from a local vendor N CAUTION Do not install racks tubing or any accessories until the prelim inary checkout has been completed Should the fraction collector fail any preliminary checkout step contact the factory or your factory authorized representative 1 Set the fraction collector upright on a level surface 2 Remove any wrapping or packing materials from the frac tion collector The arm and diverter valve assembly should move freely 3 Loosen the diverter valve thumb screw s and raise the diverter valve to its highest position Figure 2 1 Tighten the thumb screw s to secure the diverter valve 4 Connect the fraction collector to AC mains power using the supplied power cord This places the fraction collector in the Standby mode The display should be off 5 Touch the touch screen display to turn on the fraction col lector The drop former will find the home position left rear position and the touch screen display backlight will turn on The display will briefly show the current rack con figuration and then the main menu High Flow Standard Valve Figure 2 1 Adjusting the height of the standard left and high flow right diverter valves 2 2 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use TH 227 iu ali PI M Note Appendix A contains an icon g
50. diagram 1 8 serial RS232 1 10 D damage claims 2 1 5 4 delay volume calculating 3 12 compensation 1 6 detector cable Isco 1 10 non Isco 1 11 rear panel connector 1 9 dimensions physical 1 6 diverter valve fluid connections 2 5 2 7 height adjustment 2 2 2 12 drip tray cleaning 5 2 drain tubing connection 2 9 removal 5 2 drop counter cleaning 5 3 glass sleeve 5 3 drop counting general 1 2 settings 3 11 drop former calibration 3 5 E error messages 4 17 ethernet communications 4 1 rear panel connector 1 8 settings 3 6 external peak detection 3 9 F factory service 5 4 features 1 4 ferrules 2 6 2 8 firmware version 3 9 fittings swaged 2 6 2 8 flow delay when using pump counts 3 15 Index 1 Foxy R1 and Foxy R2 Fraction Collectors Index Index 2 flow delays 3 12 flow rate maximum 1 6 fluid path cleaning 5 2 fluids collect or divert 3 16 3 18 funnel racks 1 4 G glass sleeve 5 3 H hardware version 3 9 high flow valve 1 1 1 6 connections 2 7 height adjust 2 2 no drop counting 1 2 illustrated parts breakdown B 1 injection options 3 6 input signals connector pins 1 8 to 1 11 peak options 1 2 peak voltage setting 3 9 specifications 1 7 inspection preliminary 2 1 rack grounding 2 11 tubing 5 1 installation qualification IQ checklist 2 13 intersection Venn diagram symbol 3 19 IP address and mask 3 6 L
51. ducts therefore specifications are subject to change b No rack is required for R1 which holds 9 bottles on the drip tray R2 re quires a set of two racks which hold a total of 18 bottles e e o imum 3 3 to 5 VDC or contact closure active low 30 ms minimum 5 VDC max 0 to 1 0 V nominal 1 2 to 1 2 V usable 5 to 5 V max Output is open collector active low 15 VDC 100 mA maximum 300 millisecond output is open collector active low 15 VDC 100 mA max Foxy9 R1 Foxy R2 Fraction Collectors Section 1 Introduction 1 5 Rear Panel Connectors The rear panel connectors are used to power the fraction collector and connect it to other devices Table 1 3 Rear Panel Connectors Connectors Item Number Description Ethernet 1 Connector See Table 1 4 Ethernet 2 Connector Table 1 4 Pump Connector Table 1 5 Detector Connector Table 1 6 RS 232 Serial Connector Table 1 7 AC Mains Power Mains power must meet the voltage fre A quency and amperage requirements listed on the serial number label Mains power fluctuations must not exceed 10 of the nominal supply voltage The mains power cord is the disconnect A device Table 1 4 Ethernet Connector Pins Connector Pin Description Output Input TX RX auto MDI MDIX Output Input RX auto MDI MDIX Input Output RX TX auto MDI MDIX Termination Termination Inpu
52. e EN61000 4 6 Conducted RF on AC lines 150 kHz to 80 MHz A Level 1 1V rms 80 modulated EN61000 4 11 Voltage Dips Interruptions 100 drop 10ms A CISPR11 RF Emissions Group 1 Class A Industrial Scientific and EN 55011 Medical Equipment The undersigned hereby declares that the design of the equipment specified above conforms to the above Directive s and Standards as of October 27 2008 USA Representative William Foster 409 TELEDYNE ISCO Vice President of Engineering A Teledyne Technologies Company 60 2132 663 m B RT HBWYIA B JL AWD HAM EH Name and amount of Hazardous Substances or Elements in the product ARA RARIK BES En Hazardous Substances or Elements Component Name E Ed E Ti Z RIKA 219 Pb Hg Cd m AREAS X O O O O O Circuit Board X EJ x o o o e LCD Display O O O O X O Wiring Xm pg ge e X Internal Cables EERE o o o X o Line Cord X O O O X O Transformer i ig EB j X O O X E x o o x o Keypad m P8 88 S ENS 8 Name and amount of Hazardous Substances or Elements in the product TEASE PIS RI TEST O Represent the concentration of the hazardous substance in this component s any homogeneous pi
53. e Teledyne Isco does not have an authorized dealer Customers in countries outside the USA where Teledyne Isco has an authorized dealer should contact their Teledyne Isco dealer for warranty service Before returning any instrument for repair please call fax or e mail the Teledyne Isco Service Department for instructions Many problems can often be diagnosed and corrected over the phone or by e mail without returning the instrument to the factory Instruments needing factory repair should be packed carefully and shipped to the attention of the service department Small non fragile items can be sent by insured parcel post PLEASE BE SURE TO ENCLOSE A NOTE EXPLAINING THE PROBLEM Shipping Address Teledyne Isco Attention Repair Service 4700 Superior Street Lincoln NE 68504 USA Mailing Address Teledyne Isco PO Box 82531 Lincoln NE 68501 USA 800 775 2965 lab instruments 866 298 6174 samplers amp flow meters Sales amp General Information 800 228 4373 USA amp Canada Phone Repair service Fax 402 465 3001 Email February 28 2012 P N 60 1002 040 Rev G IscoService teledyne com 4 TELEDYNE ISCO Everywhereyoulook
54. e rack for 13 mm diameter tubes Holds 144 tubes 60 2137 151 One rack for 16 mm diameter tubes Holds 100 tubes 60 2137 152 One rack for 18 mm diameter tubes Holds 72 tubes 60 2137 153 One rack for 25 mm diameter tubes Holds 36 tubes 60 2137 156 One rack for 28 mm diameter scintillation vials Holds 36 vials 60 2137 154 One rack for 1 5 mL microcentrifuge tubes Holds 60 tubes 60 2137 157 One rack for 50 mL centrifuge tubes Holds 36 tubes 60 2137 159 One rack for 96 well microplates Holds 2 microplates for a total of 192 wells 60 2137 158 One rack for MiniVials Holds 72 MiniVials 60 2137 139 Foxy R2 Only One rack for 96 well microplates Holds 6 plates for a total of 576 wells 60 5247 004 Foxy R2 Only Set of two racks for 480 mL glass bottles Each rack holds nine bottles for a total of 18 60 2137 062 Case of 40 glass 480 mL bottles for 60 5247 004 on the Foxy R2 On the Foxy R1 nine of these bottles may be placed directly on the drip tray no rack is required These racks are chemically resistant made from stainless steel and polypropylene The racks accept standard sized user sup plied tubes vials and microplates of any material When in place these racks are electrically grounded This safety feature dissipates electrostatic charges when collecting organic solvents at high flow rates 1 3 Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction 1 3 Features 1 4 For nearly unlimited
55. e unit to indicate completion of the preceding commands that take some time to perform The response from the unit will be READY when the task is complete For example if you have sent RACK 1 RSVP the unit moves the drop former over the rack and then responds READY Example Command RSVP Response READY STATUS The unit will respond to this command with the last warning or failure code that occurred If an action is in progress the unit will wait to respond so a response of STATUS 0 means the preceding commands are complete and no errors occurred For example the command string STATUS 0 RACK 1 TUBE 1 STATUS will cause the unit to respond with STATUS 0 after the unit has successfully moved to tube 1 The possible status codes are 0 No Failure 1 Failure to home inward minor axis 2 Failure to home inward major axis e Failure to home inward both major and minor axes 4 Failure to home outward minor axis 5 Failure to home outward major axis 6 Failure to home outward both major and minor axes 7 Failure of major axis position sensor 8 Failure of minor axis position sensor 9 Failure of major axis position correction 10 Failure of minor axis position correction 11 Failure of both major and minor axes position correction Example Command STATUS Response STATUS 0 The STATUS 0 command clears the last warning or failure code from memory so that future errors can be noted Example Command STATUS 0 Re
56. eces is lower than the ST standard limitation X RABE EUIS S P EVE 3 15 o MEL THESEUS ER EE ER A TEHA APES EM ER PIT X RRR A THES UA X Represent the concentration of the hazardous substance in this component s at least one homogeneous piece is higher than the ST standard limitation Manufacturer may give technical reasons to the X marks MR FIERI ER ZEUG WS XE e The Environmentally Friendly Use Period EFUP was determined through experience EF B HIR RI TER EJ HE Mm Bl CE F207 48 5820075 BERIN A A B ZA 3 The date of Manufacture is in code within the serial number The first three numbers are the year of manufacture 207 is year 2007 followed by a letter for the month A is January B is February and so on Foxy 200 Hazmat Table 60 2133 635 Rev Teledyne Isco One Year Limited Factory Service Warranty This warranty exclusively covers instruments providing a one year covering parts and labor Any instrument that fails during the warranty period due to faulty parts or workmanship will be repaired at the factory at no charge to the customer Teledyne Isco s exclusive liability is limited to repair or replacement of defective instruments Teledyne Isco is not liable for consequential damages Teledyne Isco will pay surface transportation charges both ways within the 48 contiguous United States if the instrument proves to be defective within
57. eck ist ein Warnzeichen das Sei vor gefahrlichen Spannungen im Inneren des Produkts warnt Esta se al le advierte sobre la importancia de las instrucciones del manual que acompa an a este producto gt gt Esta se al alerta sobre la presencia de alto voltaje en el interior del producto xi Foxy R1 and Foxy R2 Fraction Collectors Safety xii Foxy R1 and Foxy R2 Fraction Collectors Section 1 Introduction The Foxy R1 and R2 fraction collectors are liquid distribution instruments for indoor laboratory applications The fraction col lectors have the ability to divide the liquid into multiple col lection vessels switching vessels on time drops volume peaks or a combination of these parameters Collection vessels can be test tubes vials mini wells bottles and funnels The symbolic touch screen display simplifies set up for a variety of collection routines The fraction collector is available in two models The basic model Foxy R1 holds a single collection rack and is well suited for applications with limited collection capacities The user can con figure the basic fraction collector to use different types of racks The full featured model Foxy R2 holds two collection racks and is well suited for applications with larger collection capacities or where racks are frequently interchanged with different types The Foxy R2 fraction collector uses Radio Frequency Identifi cation RFID technology to a
58. ection Peak width detection can be used alone or combined with threshold detection You can control these options using the icon in the upper right corner of the display LOO The None icon left indicates that peak width detection is Off The Intersection Venn diagram icon middle indicates that both peak width detection and threshold detection must be true before the fraction collector isolates the peak This is also considered a logical AND The Union Venn diagram icon right indicates that when either peak width detection or threshold detection are true fraction collector isolates the peak This is also considered a logical OR If you are Foxy9 R1 and Foxy R2 Fraction Collectors Section Programming using only peak width detection select this Union option Touch the icon to toggle the selection M Note If you are using only peak width detection select either the Union OR or Intersection AND option Ensure that the threshold detection is set to None 5 Touch the Enter icon to return to the Method Settings menu About Combining Peak Detection Options The Union and Intersection icons allow you to combine peak detection methods If you have programmed both peak detection methods this setting will determine whether both or any one of the peak conditions must be true Refer to the diagrams below for a comparison Intersection AND The fraction collector cuts peaks 2 and 3 where the input signal exceeds
59. ed to the fraction collector When under remote control all run time controls may also be accessed from the touch screen dis play however programming and configuration settings cannot be modified Touch this icon to terminate the remote connec tion and operate the fraction collector from the touch screen display This will return you to the main menu from which you can also access the configuration and programming menus The Lock icon indicates that a remote device has exclusive control of the fraction collector You must operate the fraction collector from the host device Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts A 2 Touch Screen Menu Flow Charts Figures A 1 through A 4 illustrate the fraction collector menus Thu p Vu max UU e gt cam e Figure A 1 Configuration settings Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts e dii TE e Figure A 2 Configuration settings continued A 7 Fo dici icd ME ctio Appendix n Glossary and Menu Cha e Figure A 3 Programming displays for the current method A 8 Foxy9 R1 and Foxy R2 Fraction Collectors
60. er the diverter valve so that the drop former is close to the top of the collection tubes Figure 2 14 Generally a 1 2 inch or 1 cm space between the drop former and the top of the tubes will sufficiently allow unobstructed movement of the arm Tighten the thumbscrew s to secure the diverter valve position Move the diverter valve over the tubes in each corner of the rack to ensure there is clearance Turn on the fraction collector by touching the touch screen display The arm will return to the home position The drop former should clear the top of the tubes d ri i Figure 2 14 Diverter Valve adjusted with 1 2 inch or 1cm clearance standard diverter valve shown 2 12 Foxy R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 2 5 Installation Qualification Checklist Installer Operator Description Initials Initials Table 5 1 Installation Qualification Checklist 2 1 Preliminary Checkout 2 2 1 Standard Diverter Valve 2 2 2 Connections or High Flow Diverter Valve Connections 2 2 3 Drip Tray Drain Connection 2 8 Hack Installation 2 4 Diverter Valve Adjustment Certification of Section 2 Completion Installer Name print Installer Signature Date Operator Name print Operator Signature Date Comments 2
61. erter valve to its lowest position refer to Figure 2 1 This will expose the ports on the back of the diverter valve Tighten the thumb screw to secure the diverter valve 2 5 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use Figure 2 7 Tubing preparation 2 6 4 The accessory package includes a 10 ft length 3 m of tub ing Cut a length of tubing to connect between the In port and the source M Note Cut the tubing with a tubing cutter to ensure that the end is cut cleanly and free from any deformation 5 Prepare the tubing as shown in Figure 2 7 10 Tapered edge of lock ring must face the yellow ferrule ii 2 58 E a Pe 5 LE HE I 209 0163 21 l L Insert the tubing into the In port With the tubing fully seated thread the headless nut into the port until fin ger tight Unthread the headless nut and inspect the fitting The fer rule should be fully swaged at the end of the tubing The following should be observed the yellow ferrule should be flush and perpendicular with the end of the tubing the metal lock ring should be compressed over the ferrule without any gaps all pieces should be correctly aligned and free from any deformation If not fully swaged reinsert the headless nut into the port and tighten further Remove the headless nut and inspect again using the criteria above Repeat steps 4 through 7 to prepare the tubing for
62. film or coatings might prevent their ability to dissipate static electricity To avoid problems that possibly result from an electrostatic discharge clean the racks and tray monthly Use distilled water with a mild detergent For tougher stains use isopropyl alcohol The drip tray can be removed for thorough cleaning It is held in place by pins First remove the collection racks and any drain tubes Pry the corners of the drip tray loose with your fingers After loosening all four corners lift the drip tray up and off of the instrument case Clean the drip tray with mild detergent To replace the drip tray place the tray on the instrument aligning the drain with its hole Press down on the tray firmly to seat it onto the pins To clean the exterior surfaces use a cleaning cloth dampened with a mixture of distilled water and a mild detergent Use iso propyl alcohol for tougher stains On printed areas such as labels avoid rubbing vigorously or using aggressive solvents like acetone Each will ruin the printed text Quickly clean up any aggressive solvents spilled on the exterior surfaces The paint protects the underlying metal from corrosion Aggressive solvents might also damage the plastic covers which protect internal components Z CAUTION Do not immerse the instrument in a water bath or subject it to a liquid spray The instrument is not watertight and these actions could damage the internal electronics Precipitates
63. fore attempting to assemble or operate the fraction collector ensure that all parts including tube racks or funnels listed on the packing slip are in the shipping carton s N CAUTION Never lift the fraction collector by the arm Permanent damage the arm mechanism may occur Note Optional accessories ordered with the fraction collector might be shipped in a separate carton Inspect the fraction collector for damage that might have occurred during shipping Notify the shipping carrier immedi ately if you find any damage Before using the fraction collector you should perform a prelim inary checkout to ensure it functions properly This includes testing electrical mechanical and programming functions do this enter a simple program and operate the fraction collector WARNING The AC mains power cord is the disconnect device Position the fraction collector so that the power cord can be unplugged or use a power strip where the plug can quickly be removed from the outlet in the event of an emergency WARNING Refer to the serial number label for mains power requirements 2 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use M Note To perform the preliminary checkout you will need to power the fraction collector The fraction collector is shipped with either a North American IEC320C13 to NEMA 5 15P power cord or a European IEC320C13 to CEE7 VII power cord If the cor
64. gical OR Touch the icon to toggle the selection Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming M Note If you are using only threshold detection select either the Union OR or Intersection AND option Ensure that the peak width detection is set to None 5 Touch the Enter icon to return to the Method Settings menu 3 3 6 Peak Width Detection Peak Width detection isolates small and large magnitude peaks The Peak Width is set as the average time duration of the expected peaks The fraction collector monitors the slope of the input signal Peak width detection is also commonly called Slope detection If the rising signal is consistent with that of a peak the fraction collector isolates the fluid In a similar way the falling slope as it transitions to the baseline indicates the end of a peak The internal slope detection algorithms are effective in isolating multiple shouldered peaks peaks that do not return to the baseline before the next peak occurs The peak width option is useful to eliminate false peaks that can result from a rising or noisy baseline An example of Peak Width detection and collection is shown in Figure 3 15 The peaks on the analog signal are numbered 1 through 4 The fraction collector isolates peaks 1 through 3 in collection tubes because of the slope of the peak signal Peak 4 is ignored because the signal does not indicate a slope within the programmed peak width
65. he Enter icon to save the setting and return to the previous display The Inject or Timed restart options are combined with a col lection tube option Overlay Next or Skip These options are illustrated in Figure 3 6 Overlay If you select the overlay option the fraction collector moves the drop former back to the original tube position That is at every restart the drop former returns to the tube position from which the run was started 53 53 Restart 2 S2 52 Restart 1 OG li jg 1 Restart Restart 1 2 NA Restart Restart 1 2 Figure 3 6 Overlay Next and Skip options diagram 3 8 3 2 5 Pump Control Settings o go 3 2 6 Analog Peak Settings J 3 2 7 Fraction Collector Information Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming One example of this tube conserving option s usefulness is to collect like eluate from multiple identical samples while repeating the same liquid chromatography method Because the peaks elute the same each time greater volumes of pure com pound can be collected in a tube Next The Next option continues with the next available tube at each restart Skip The Skip option skips one tube and continues with the following tube at each restart The empty tube helps you locate the sets of tubes for each restart The fraction collecto
66. he fraction collector arm moves along the Y axis to position the arm over the correct row The arm includes electromechanical com ponents move the diverter valve along the X axis to position the drop former counter over the collection vessel The drip tray catches fluid when the collection rack is mis config ured in the software when collection tubes are missing and when collection tubes are over filled The rack guide pins ensure the racks and funnels are correctly positioned under the diverter valve assembly Some guide pins also serve as an electrical ground to dissipate static electricity that can build up when collecting organic solvents at high flow rates The adjustable foot allows you to level the fraction collector The drip tray drain can route overflow fluids to a user supplied col lection container or drain A secondary drain routes any fluids that miss the drip tray to a common collection point Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction Figure 1 2 Fraction collector features Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction 1 4 Technical Specifications Table 1 2 lists the fraction collector technical specifications Table 1 2 Specifications Dimensions width depth height Foxy R1 PN 68 3870 010 12 25 13 0 14 0 in 31 1 33 0 35 5 cm Foxy R2 PN 68 2130 006 12 25 21 0 14 9 in 31 1 53 3 37
67. hile in manual control INVALID COMMAND One of three situations could cause this response An unknown command has been entered usually a typographical error Anoperand has been sent for a command that does not require one Anoperand is missing for a command that requires one INVALID The operand value is out of range or unsupported LOCAL MODE unit must be in the remote mode to do anything other than read values 4 17 F oxy9 R1 and R2 Fraction Collectors Section 4 Serial Command Control 4 18 Foxy R1 and Foxy R2 Fraction Collectors Section 5 Maintenance 5 1 Introduction 5 2 Maintenance 5 2 1 Tubing Inspection The fraction collector is designed for years of use in typical labo ratory applications The recommended maintenance tasks pri marily address the fluid path drains and the fraction collector exterior These tasks should be performed as needed to ensure the optimum performance of items such as the diverter valve and drop counter as well as protect the exterior finish Its mechanical parts do not require periodic adjustment or lubrication The fraction collector does not contain user serviceable parts Should your instrument require service refer to section 5 3 for assistance Perform the following tasks as necessary Tubing Inspection section 5 2 1 Rack and Drip Tray Cleaning section 5 2 2 General Cleaning section 5 2 3 Fluid Path Cleaning
68. icon again will return the fraction collector to the Configuration Tools menu Touching it once more will display the Main menu The fraction collector can vary the speed of the tube advance motion Higher flow rate or higher viscosity liquids may cause some liquid to miss the tube opening during tube changes The Tube Advance Speed setting allows you to minimize this effect To adjust the Tube Advance Speed 1 Touch the Tube Advance Speed Rabbit icon 2 The left side of the display shows the speed setting Increase or decrease the speed by touching the rabbit faster icon or the turtle slower icon 3 Touch the Enter icon to save the calibration settings The collection pattern icon displays the selected pattern either left to right or serpentine Touch the icon to toggle the selections Calibrate 3 2 2 RS 232 Serial Settings Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming The factory calibration positions the drop former over the col lection tubes for all available rack types Calibration is not nec essary for most applications and commercially available collection vessels Some applications or collection vessels require calibration to further refine the drop former position This need is evident when the drops fall on or beyond the rim of the collection vessel M Note If the only drops that fall on or beyond the rim are those imme diately following a tube advance the problem is not due to ca
69. ignal Output Pump pause stops pump between tubes and at end of run Output Valve output control signal for optional high flow diverter valve Common Circuit common Output 24 VDC Output power for optional high flow diverter valve Not used Shield Earth ground A cable is available to connect the fraction collector to a non Isco detector Part number 69 2134 171 is a six foot long cable with one end terminated as a 8 pin mini DIN connector The other end is stripped and tinned for connection to the external device Table 1 9 Non Isco Detector Cable P N 69 2134 171 Connector Pin Color Type Common Not used Description Analog common for use with pin 8 Ext peak Output Event pulse delivered at tube change Input Inject starts an inject cycle Input Tube Advance Common Circuit common Input Analog detector signal used for peak detection Shield Earth ground Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction Foxy R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 2 1 Preliminary Checkout This section describes the setup procedures for the fraction col lector and its optional accessories M Note Section 2 5 contains an Installation Qualification checklist If required sign off the checklist entries as you successfully complete the following sections Be
70. iguration settings 3 3 grounding 2 11 installation 2 10 part numbers 1 3 RFID detection Foxy R2 2 10 specify last tube 3 4 remote mode 3 1 4 2 4 4 Foxy amp R1 and Foxy R2 Fraction Collectors Index renaming a method 3 11 U replacement parts B 1 union Venn diagram symbol 3 19 restarts 3 6 unpacking 2 1 returns 5 4 RFID 2 10 defined 1 1 Venn diagrams 3 16 3 18 3 19 racks and funnels 1 4 RS232 serial volume pump count communications 4 1 general 1 2 connector 1 10 settings 3 14 settings 3 5 volumes by drop counts 3 12 S by pump counts 3 14 by time intervals 3 13 serial RS232 communication settings 3 5 Ww communications 4 1 connector 1 10 serial commands immediate control 4 6 miscellaneous 4 2 problem responses 4 17 programming 4 9 syntax 4 2 service 5 4 shipping 5 4 skip tube option injection or timed restarts 3 9 slope detection see peak width detection specifications 1 6 speed tube advance 3 4 swaged fittings 2 6 2 8 syntax serial commands 4 2 system control options 1 7 warranty service 5 4 waste and peak fluids 3 16 3 18 weight 1 6 wetted materials 1 7 windows time 3 20 T TCP IP settings 3 6 telnet 4 1 temperature operating 1 7 threshold detection general 1 2 settings 3 15 time intervals general 1 2 settings 3 13 time windows general 1 2 menu 3 21 settings 3 20 timed restarts 3 6 tools configuration 3 3 tube adv
71. in Appendix A Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming 3 1 Main Menu All programming via the touch screen begins at the Main Menu Figure 3 1 4 Configuration Tools TT i Es Method Settings le Collect in Tubes ll Divert to Waste Tube Position HNH Tube Advance HAR Play start method Standby Access the fraction collector programming by touching either the Tools icon or the Folder icon Figure 3 1 Main Menu Touch the Configuration Tools icon to view or TH modify the fraction collector Configuration set tings such as the rack type input output settings etc The Configuration settings should be reviewed and set as required when the fraction collector is first installed and whenever the configuration is changed thereafter Refer to Section 3 2 for details Touch the Folder icon to view or modify the Method settings The Method settings direct the operation of the fraction collector after you touch the Play icon These settings define tube change intervals peak cutting time windows etc Up to eight different methods can be defined one of which is always current For more details see Section 3 3 The other Main Menu icons are briefly discussed below Ta Touch the Diverter Valve Position icons to toggle j ll the position of the diverter valve When the arrow points to the tube the valve is set to col lect fluids in tubes When the arrow points to the waste ca
72. ion collector always combines peak detection with a uniform mode so that it does not exceed the tube capacity while a peak is active The fraction collector can also limit the fraction collection to certain time durations during a collection routine The Time Windows collection scheme is described below 1 2 Racks and Vessels Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction Time Window The fraction collector can collect fluids within time windows This collection mode allows the user to col lect or reject fluids based on time For example the time window can start one minute into the run to divert initial waste fluids and then end ten minutes into the run to reject any late eluting peaks that might occur during a five minute column flush In other words fractions will only be collected for the nine minutes of the run which deliver frac tions of interest Up to four time windows may be combined with a uniform col lection mode and with peak detection The fraction collector is designed to accept a variety of racks The racks align the collection tubes minivials and mini well plates under the drop former and allow for quick removal and replacement The factory ships the Foxy R1 fraction collector with one rack the Foxy R2 fraction collector ships with two The following racks are available 60 2137 155 One rack for 12 mm diameter tubes Holds 144 tubes 60 2137 150 On
73. its no parity 1 stop bit and no flow control Communication with the fraction collector can be established through either Ethernet port These connections use a Category 5 or higher cable The fraction collector will automatically detect straight and cross over cables and configure the communi cation accordingly Refer to Section 3 to configure the TCP IP settings of the fraction collector The fraction collector uses port 23 for remote control and commu nication Although the fraction collector uses port 23 commands spe cific to TELNET protocol will be ignored ASCII printable characters are transmitted as 8 data bits no parity bit and 1 stop bit 1 LabVIEW is a trademark of National Instruments Corporation 4 1 Foxy9 R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 4 2 Command Syntax 4 4 3 Line Syntax 4 4 4 Modes 4 4 5 Errors 4 4 6 Timing 4 5 Serial Commands 4 2 4 5 1 Miscellaneous Commands Commands consist of single keywords possibly followed by an operand separated by a for example BAUD or TUBE 42 Commands may be abbreviated to as few as three characters Spaces are ignored Backspace ASCII character 8 may be used to correct mistakes Commands are not case sensitive Commands are processed when a carriage return is received Multiple commands may be sent on a line separated by semi colons for example NEXT NEXT RSVP Total line length must n
74. l ibration Instead lowering the drop former section 2 4 or adjusting Tube Advance Speed might prevent this condition Additionally consider stopping the fluid delivery pump during tube changes Section 3 2 5 Should calibration be necessary 1 Ensure that the fraction collector is level 2 Load the rack with the collection vessels and place the rack on the system 3 Touch the Calibrate icon to access the calibration options 4 Touch the First Tube icon to move the drop former over the front and left most tube in the rack 5 Touch the Arrow buttons to incrementally position the drop former over the center of the collection vessel opening M Note The white diverter valve housing has a arrow on the face to indicate the center of the drop former outlet This can assist with the left to right X axis positioning 6 When the first tube position is calibrated touch the Last Tube icon to move the drop former over the back and right most tube 7 Touch the Arrow buttons to position the drop former over the center of the collection vessel opening 8 Touch the enter icon to save the calibration settings By calibrating the drop former position over the first and last tubes the fraction collector calculates the position of all other tubes in the rack External devices can communicate with the fraction collector One option is through the RS 232 Serial port Many of the com munication parameters are predefined by the fir
75. le Command PROGRAM Response PROGRAM 8 Foxy9 R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 12 RANGE n This command sets the detector signal voltage range of the current program The choices are n 1 1 Volt n 2100 mV e n 3 10 mV e n 4 External detector Example Command RANGE 2 Response none The RANGE command without an operand returns the current setting Example Command RANGE Response RANGE 2 RESTART n This command sets the restart action of the current program Choices are e n 1 None n 2 Inject Overlay e n 3 Inject Skip Tube e n 4 Time Overlay e n 5 Time Skip Tube n 6 Inject Next Tube n 7 Time Next Tube Example Command RESTART 5 Response none The RESTART command without an operand returns the current setting Example Command RESTART Response RESTART 5 RSTIME n This command sets the restart time n 0 to 359999 seconds of the current program Example Command RSTIME 600 Response none F oxy9 R1 and R2 Fraction Collectors Section 4 Serial Command Control The RSTIME command without an operand returns the current setting Example Command RSTIME Response RSTIME 5000 RILASTzn or R2LASTzn These commands set the last tube for Rack1 or Rack2 Choices are n 1 to n maximum number of tubes for programmed rack type Example Command R2LAST 10 Response none The R1LAST or R2LAST command without an operand return
76. lossary for complete descrip tions of the icons used in the preliminary checkout procedure Figure 2 2 Main menu 6 Touch the Folder icon twice to open a list of collection methods Method A should be selected If not touch the up or down arrows to highlight Method A 2 Figure 2 3 Method Select screen 7 Touch the Enter icon to accept Method and return to the screen at which you can edit the method settings 2 3 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use Figure 2 4 Edit Method Settings screen Figure 2 5 Time based Fractions screen 2 4 8 10 11 12 13 14 e p 6 Touch the Time icon to display a screen at which you modify the fraction collection based on time The fraction size option should be selected That is a box should appear around the tube If not touch the Volume Select icon to toggle between the options Use the Arrow icons to set the interval to two seconds Touch the Enter icon twice to save the setting and return to the main menu Touch the Play icon to start operation The arm and drop former will move to the first tube position and then advance to the next tube every two seconds Allow the drop former to advance through at least 20 tubes to ensure that the arm freely moves through the X and Y axes Touch the Pause icon The arm and drop former should stop over
77. mware e Supports ASCII printable characters e 8 bit No parity bit e 1 stop bit 3 5 Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming 3 2 3 TCP IP Settings 3 2 4 Inject Options V Inject or timed None 3 6 The user configurable parameter is the baud rate To set the baud rate 1 Touch the RS 232 Serial settings icon 2 Select the desired baud rate from the list using the Up and Down Arrow buttons 3 Touch the Enter icon to save the selection and return to the Configuration Tools menu M Note Refer to Section 4 for more information on communications with external devices External devices may communicate with the fraction collector using TCP IP protocol and a static IPv4 address To configure the TCP IP settings 1 Touch the TCP IP settings icon to display the communica tion options 2 Review the IP and IP Mask values To change either value a Touch the value you intend to modify b Touch the Up and Down Arrow icons to set the numeri cal value at the cursor position c Move the cursor position by touching the Left and Right Arrow icons d Touch the Enter icon to save the setting and return to the previous display M Note Should you need assistance with determining the correct static IP address and IP Mask values contact your IT department M Note The view only MAC address is unique to each fraction collector and cannot be changed Inject options also known a
78. n In the calibrate mode the arrow icons finely calibrate the drop former position Touch the Collection Pattern icon to toggle between left to right and serpentine patterns Touch the Maximum Tube Number icon to specify the last tube in a rack The default value is the maximum capacity of the selected rack Users can specify a lesser value if the rack is not filled completely with empty tubes or to end the run sooner Touch the Serial Port icon to open a screen from which you can configure the serial communica tion settings Foxy9 R1 and Foxy R2 Fraction Collectors Appendix A Icon Glossary and Menu Charts Table A 1 Touch screen display icons Continued Icon Description Touch the Ethernet Port icon to open a screen from which you can configure TCP IP communi cation settings and view the MAC address Touch the Inject icon to open a screen from which you can select the restart action for each injec tion Restarts can be initiated by a user specified Time or an Inject signal from an external device When using one of the Inject options the fraction collec tor waits for the first restart event before starting the method The touch screen Play icon will alter nate with the Inject icon while waiting After the injection touch the Play icon to continue with the method Timed restarts occur without interven tion When a restart occurs the collection pattern can return to the first tube of the previous
79. n the valve is set to route fluids to waste Touch the Next Tube icon to advance the drop for lal mer to the next tube position The current tube position is reported next to this icon p Touch the Play icon to start the current method l Touch the Power icon to place the fraction collector C2 in the Standby state 3 2 3 2 Configuration Settings Foxy9 R1 and Foxy R2 Fraction Collectors Section Programming Configuration settings define the overall fraction collector oper ation That is these global settings are applicable regardless of whichever collection Method is selected The fraction collector displays the Configuration menu Figure 3 2 when you touch the Configuration Tools icon When you first install the fraction collector these settings should be reviewed and set as necessary Also update the settings whenever the configuration changes Sections 3 2 1 through 3 2 7 provide details for each of the Configuration Tools menu options 3 oo Enter Rack Settings RS 232 Serial Settings TCP IP Settings Inject Options Pump Control Settings gt eae T e Analog Peak Settings Fraction collector information Figure 3 2 Configuration Tools Menu 3 2 1 RackSettings Figure 3 3 Rack options menu Touch the rack icon to view the Rack options menu Figure 3 3 From this menu you can view or change the type of rack set the collection pattern and speed and calibrate the drop former positi
80. nector Pins Connector Pin Type Description Not used Output TxD RS 232 serial data out Input RxD RS 232 serial data in Reserved Common Circuit common Reserved Not used Not used Not used Shield Earth Ground oc N 1 6 Accessory Cable Specifications 1 6 1 Cables for Isco Cables are available to connect the fraction collector to Isco Accessories equipment To connect the fraction collector to a TRIS pump order accessory cable 69 2134 172 This cable connects the Signal output from the TRIS pump to the fraction collector s Pump connection This cable carries the pump count and pump pause signals connect the fraction collector to an Isco UV Vis detector such as the UA 6 order accessory cable 69 2134 173 This cable carries the analog peak detector signal as well as the tube advance event pulse external peak and inject signals 1 6 2 Cable for non Isco A cable is available to connect the fraction collector to a non Isco Pumps pump Part number 69 2134 170 is a six foot long cable with one end terminated as a 6 pin mini DIN connector The other end is stripped and tinned for connection to the external device Foxy R1 and Foxy R2 Fraction Collectors Section 1 Introduction Table 1 8 Non Isco Pump Cable P N 69 2134 170 Connector 1 6 3 Cable for non Isco Detectors Color Type Input Description Count pump volume s
81. nit is finished processing a command line and is ready for another This line pacing can be used by many terminal programs to avoid downloading com mands faster than the unit can execute them CUE 1 will enable the prompt CUE 0 will disable it CUE 0 is set when unit is switched to OPERATE F oxy9 and R2 Fraction Collectors Section 4 Serial Command Control Example Command CUE 1 Response gt CUE without an operand returns the current setting Example Command CUE Response CUE 1 ECHO n This command enables or disables echoing of char acters received Echoing can be used to verify correct reception of characters ECHO 1 will enable the echoing ECHO 0 will disable it ECHO 0 is set when unit is switched to OPERATE Example Command ECHO 1 Response none ECHO without an operand returns the current setting Example Command ECHO Response ECHO 1 HELP The unit will respond to this command with a list of valid command names Example Command HELP Response CLEAR COLLECT Combining the HELP command with a valid command using the symbol will return more information for select commands Example Command HELP BAUD Response sets amp queries the serial port baud rate IDENTIFY This commands returns information identifying the unit Example Command IDENTIFY Response SERIES 3870 010 FoxyR1 FRAC COL REV a 01 00 00 4 3 Foxy9 R1 and Foxy R2 Fraction Collect
82. ol PUMP n This command sets the pump controlled by the fraction collector on PUMP 1 or off PUMP 0 Example Command PUMP 1 Response none A PUMP command without an operand returns the current setting Example Command PUMP Response PUMP 1 RACK n This command instructs the unit to move the drop former over the home position RACK 0 or the racks RACK 1 RACK 2 Example Command RACK 1 Response none A RACK command without an operand returns the current position Example Command RACK Response RACK 1 RTUBE rnnn This command sets the current rack number to r and tube number to nnn and moves the drop former to it if the drop former is not at home nnn can be from 1 up to the maximum for currently programmed rack type nnn must be three characters in length Example Command RTUBE 1002 Response none An RTUBE command without an operand requests the rack and tube number the drop former is currently over Example Command RTUBE Response RTUBE 2035 4 7 Foxy9 R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 8 RUN The fraction collector starts continues or restarts a run Example Command RUN Response none RIDETECT 2 Foxy R2 only These commands query the two rack RFID readers that are connected to the circuit board R1DETECT responses with the rack identity of Rack1 front rack R2DETECT responses with the rack identity of Rack2 back r
83. on over the rack Enter c1 Tube Advance Speed Rack 2 Back Foxy R2 Only j Collection Pattern t Calibrate Lt Rack 1 Front 3 3 Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming 3 4 Rack 1 and Rack 2 Tube Advance Speed Collection Pattern M Note The two rack Foxy R2 fraction collector uses radio frequency identification RFID technology to automatically detect the rack types The Rack options menu displays the detected rack types The Rack 1 and Rack 2 options report the current Rack type On the Foxy R2 fraction collector rack types are automatically detected whenever the touch screen display returns to the Main menu Therefore setting the rack type for the Foxy R2 is not nec essary To change the rack type not recommended on Foxy R2 fraction collectors 1 Touch the Rack 1 or Rack 2 settings to open a list of sup ported rack types 2 Select the desired rack type from the list using the Up and Down Arrow buttons M Note The selected rack type should match the installed rack Other wise fluids may miss the collection tube openings 3 If you intend to use fewer tubes than the capacity of the rack touch the Number of Tubes icon Use the Arrow icons to specify the last tube in the rack then touch the Enter icon to accept the value 4 Touch the Enter icon to save the selection and return to the Rack Options menu M Note Touching the Enter
84. ors Section 4 Serial Command Control 4 4 LINEFEED This command enables LINEFEED 1 or disables LINEFEED 0 the sending of a linefeed character each time a carriage return is sent LINEFEED 0 is set when unit is switched to OPERATE Example Command LINEFEED 1 Response none LINEFEED without an operand returns the current setting Example Command LINEFEED Response LINEFEED 0 LOCAL This command sets the unit to local mode In local mode serial commands cannot affect the unit s operation com mands can only obtain information Local mode is set when the unit is switched to OPERATE See REMOTE on page 4 4 Example Command LOCAL Response none NOTE text or NOTE This command and any following text are ignored by the unit This allows annotating program files for user reference NOTE without a operand can provide spacing for readability Example Command NOTE Use with column 3 at 3 mL min Response none REMOTE This command sets the unit to remote mode The unit must be put into remote mode before any commands are sent that affect the unit See also LOCAL on page 4 4 The touch screen on the unit is still operational in remote mode You can return the fraction collector to the Local mode from the touch screen by touching the computer icon Example Command REMOTE Response none F oxy9 R1 and R2 Fraction Collectors Section 4 Serial Command Control RSVP This command instructs th
85. ot exceed 80 characters Errors occurring from any command do not stop the execution of the remaining commands on line The fraction collector must be in remote mode to do anything other than read values See REMOTE on page 4 4 for more infor mation Program parameters cannot be changed while a run is in progress Also changes to any parameter currently being edited on the fraction collector display will not be effective Errors relating to the syntax of commands result in problem message responses See Problem Responses on page 4 17 Hardware related errors warnings and failures are indicated by the display and audio output of the unit and can be detected serially using the STATUS command The fraction collector s response time to most commands is 50 milliseconds One exception is commands that require some mechanical action such as arm movement Mechanical actions normally do not exceed 4 seconds Other exceptions are noted where applicable The following is a list of all fraction collector serial commands with a brief description and example of the use for each command For some commands no response is required or expected from the unit The examples show this as Response none CLEAR This command resets the unit It is equivalent to switching the unit to standby and then back to operate Example Command CLEAR Response none CUE n This command enables or disables the sending of a gt prompt character when the u
86. placement Parts Listings v2 WILI 30 19 SEB HIGH FLOW FOXY R2 DRAWING NO E 602132669B SHEET 5 OF 12 B 29 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings HIGH FLOW FOXY R2 DRAWING NO 602132669B SHEET 6 OF 12 B 30 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix Replacement Parts Listings SJ fe HIGH FLOW FOXY R2 DRAWING NO 6021326698 SHEET 7 OF 12 B 31 Foxy R1 Foxy R2 Fraction Collectors Appendix Replacement Parts Listings HIGH FLOW FOXY R DRAWING NO 602132669B SHEET 8 OF 12 B 32 Foxy R1 and Foxy9 R2 Fraction Collectors Appendix B Replacement Parts Listings ID 1 Mili SEE DETAIL S DETAIL RA SCALE 0 625 XLT COR N t SEE DETAIL F 2 ONANAN SA 0 S h gt T lt gt
87. r briefly diverts fluid to waste until it finishes the run with the end of Time Window 2 Percent Time Window 1 Window 1 Window 2 Window 2 Start End Start End Figure 3 17 Time Windows limit the collection to specific 3 20 time durations To program Time Windows 1 Touch the Time Windows icon from the Method Settings menu to open the menu Figure 3 18 Foxy9 R1 and Foxy R2 Fraction Collectors Section Programming Y A Figure 3 18 Time Windows Settings menu 2 Time Windows are either Off or combined with the selected peak detection where all conditions must be true Touch the icon in the upper right corner to select the intersection AND option M Note If peak detection mode is not used you must still select the intersection AND option to enable Time Window operation 3 The first displayed time values are for the start of Time Window 1 Enter the start time using the Arrow icons The Up and Down Arrows increment and decrement the value the Left Arrow moves the cursor position The Time Win dow Start value is the time from the start of the run press ing the Play icon 4 Touch the Start End icon to advance to the end of Time Window 1 5 Enter the Time Window 1 end time using the Arrow icons This value is the time from the start of the run 6 If you are using only one time window touch the Enter icon to save the settings and return to the Method Setting
88. r can control an external pump from pin 2 of the Pump connector This signal is used to pause the pump during tube changes Touch the Pump Control icon to view the current setting The icon with the 1 left indicates that iol the fraction collector will not send a pause signal from pin 2 In this state the external pump will continue to operate during tube changes The icon with the 0 indicates that the fraction collector will send a pause signal from pin 2 Touch the icon to toggle the state The fraction collector can receive an analog peak signal from an external detector The fraction collector receives this signal at pin 8 of the Detector connector When using this feature set the analog peak voltage scale to the range of the detector signal either 1000 mV 100 mV or 10 mV Touch the Analog Peak setting icon to view and select the range using the Up and Down Arrows Choose the voltage that corresponds to your detection device s 100 signal output If your detector does not scale its output to one of the options choose the option just above your highest expected peak voltage If you select the External option the fraction collector will ignore the signal at pin 8 and monitor the TTL signal at pin 2 instead The fraction collector begins cutting peaks when an active low is present Touch the Information icon to display Model number and hardware firmware revisions 3 9 Foxy9 R1 and Foxy R2 Fraction Collecto
89. rs Section 3 Programming 3 3 Method Settings Figure 3 7 Method Settings Menu 3 3 1 Method Selection Method settings define the fraction collector operation for a run The fraction collector displays the Method Settings menu Figure 3 7 when you touch the folder icon on Main menu Use this menu to program the current method or select another of the eight stored methods Refer to section 3 3 1 for information on selecting and naming methods Sections 3 3 2 through 3 3 7 describe how to program the current method u Enter baz Method Selection Drop Counts Threshold Detection Time Intervals E Volume Pump Counts 4 Time Windows To view the name of the current method touch the folder icon from the Method Settings menu The displayed list of methods highlights the current method name Figure 3 8 Slope Peak Detection To select a different method use the Up and Down Arrows to highlight the desired method Touch the Enter icon to accept the new method and return to the Method Settings display Figure 39 8 Method Selection Menu 3 10 You can also rename the methods After highlighting a method touch the Folder icon The fraction collector displays a text editing screen Figure 3 9 from which you can rename the method Use the Left and Right Arrows to move the cursor and the Up and Down Arrows to change the character or number When com plete touch the Enter icon to sa
90. rt Customers can find their regional dealer by visiting the Teledyne Isco Web site http www isco com Iccontact Please contact the Service Department before returning the instrument to the factory for repair Return instructions can be found on the Foreword and Warranty pages at the front and back of this manual Before packing the instrument make sure all parts and hardware are in their proper places Wrap the instrument in heavy paper or put it in a plastic bag for extra protection If at all possible pack the instrument in its original shipping container with the original packing material If the original shipping con tainer is not available place the instrument in a strong card board box at least six inches larger in all three dimensions than the instrument itself Fill the box equally around the instrument with a resilient packing material such as shredded paper bubble pack expanded foam etc Seal the box with strapping tape or other suitable shipping tape and ship it to the return address listed on the warranty page The warranty page describes the conditions under which Teledyne Isco will pay surface shipping costs for returns It is very important that the instrument be well packaged for shipment and fully insured Damage claims must be conducted between you and the carrier Damage in shipment due to inade quate packing can delay the repair and return of the instrument to you Foxy R1 and Foxy R2 Fraction Collectors
91. run to Over lay multiple fractions from a repeated sample Alternatively a restart can start at the Next tube or Skip a tube to separate the runs Touch the Pump Control icon to access the set tings to control the output at pin 2 of the Pump connector This signal can be used to pause the pump operation during tube advances The icon with the 1 left indicates that the frac tion collector will not send a pause signal from pin 2 In this state the external pump will continue to operate during tube changes The icon with the 0 indicates that the fraction collector will send a pause signal from pin 2 Touch the Analog Peak setting icon to view a list of voltage ranges for the peak input When using this feature set the analog peak voltage scale to the range of the detector signal Touch the Information icon to display Model num ber and hardware firmware revisions Touch the Drop icon to collect fractions based on the number of drops collected Touching this icon opens a screen from which you can set the delay volume as a number of drops and the number of drops to collect before performing a tube advance If selected the display shows a border around this icon Touch the Time icon to collect fractions based on time Touching this icon opens a screen from which you can set the delay volume as a time interval and the time duration of each fraction If selected the display shows a border around this
92. s menu To use more than one time window touch the Start End icon to advance to the start of Time Window 2 Repeat the steps to enter the start and end values for up to four time windows When finished touch the Enter icon to save the settings and return to the Method Settings menu M Note When programming or reviewing the Time Windows settings duplicate values for the start and end of a time window indi cates that the current time window is not used Only the previ ous time windows will be used in the method 3 21 Foxy9 R1 and Foxy R2 Fraction Collectors Section Programming 3 22 Foxy R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 1 Introduction 4 2 RS 232 Communication Information 4 3 Ethernet Communication Information 4 4 General Command Information 4 4 1 Character Format The fraction collector has built in RS 232 and Ethernet inter faces that allow it to be remotely controlled Remote control is typically done using a computer running a terminal program to send commands and receive data Software applications such as LabVIEW can automate the fraction collector operation The minimum connections required for serial control are Pin 2 TRANSMIT Serial data output to computer Pin 3 RECEIVE Serial data input to computer Pin 5 COMMON Common for all signals Available baud rates are 1200 2400 4800 9600 19200 38400 56800 and 115200 Configure the remote device for 8 b
93. s the current setting Example Command R2LAST Response R2LAST 10 RITYPE n or R2TYPE n These commands set the rack type of the current program Choices are e n 0 none e n 1 12 13 mm tubes n 216 mm tubes n 3 18 mm tubes e n 4 17 mm vials e n 5 25 28 mm vials e n 6 1 5 mL microcentrifuge tubes e n 7 36 funnel prep rack n 8 Bottles e n 9 Single Beaker n 10 Microplates 96 well x 2 n 11 Microplates 96 well x 6 n 12 26 funnel prep rack Example Command RTYPE 5 Response none The RTYPE command without an operand returns the current setting Example Command RTYPE Response RTYPE 5 4 13 Foxy9 R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 14 THRESHOLD n This command sets the peak threshold n 0 none to 100 of the current program Example Command THRESHOLD 10 Response none The THRESHOLD command without an operand returns the current setting Example Command THRESHOLD Response THRESHOLD 10 WIDTH n This command sets the peak detection width of the current program Choices are e n 1 5 seconds n 2 15 seconds n 3 30 seconds e n 4 1 minute e n 5 2 minutes e n 6 4 minutes n 7 8 minutes e n 8 16 minutes e n 9 32 minutes e n 10 1 hour e n 11 No slope detection Example Command WIDTH 2 Response none The WIDTH command without an operand returns the current setting Example Command WIDTH Response WIDTH 2 WINDOW
94. s Restart options control the oper ation of the fraction collector when collecting fractions from mul tiple samples Touching the Inject icon displays a list of options If you intend to collect fractions from a single sample after touching the Play button select the None option The fraction collector will collect fluids until the last tube or until stopped If you intend to collect fractions from multiple samples during a run choose an Inject or Timed option The difference is the means used to restart the collection of each sample Inject The inject options rely on an Inject signal from an external device The fraction collector receives this electrical pulse input on pin 5 of the Detector connector Play Play Inject Run Foxy9 R1 and Foxy9 R2 Fraction Collectors Section Programming Samples SS quee eo pom pub I Pauses Inject Signal l 1 Start of Run Restart Restart Restart 1 2 3 Figure 3 4 Inject options timing diagram Play c When first started Figure 3 4 Start of Run using one of the Inject options the fraction collector pauses so that the sample can be injected as indicated by the Play icon alternating with the Inject icon Touch the Play Inject icon after the sample injection to resume the operation The fraction collector advances through collection tubes as pro grammed by its method settings until it receives an Inject signal Fig
95. s icons to toggle through the detection modes The top icon indicates that peak width detection is OFF The middle Union icon indicates that peak width detection is enabled If the slope of the input sig nal indicates a peak the peak state will be true regardless of any other detection mode a logical OR The bottom Intersection icon indicates that peak width detection is enabled If the slope of the input signal indicates a peak the peak state is true only when other enabled detection condi tions are met a logical AND Touch the Time Windows icon to collect fluids within a time window A border around this icon indicates that at least one time window is pro grammed and enabled Touch the Time Window Options icons to toggle modes The left icon indicates that the Time Win dows mode is OFF The right Intersection icon indicates that a time window is enabled If a threshold or peak width detection condition is true and the time window condition is true the peak state is also true a logical AND Each touch of the Time Windows Start Stop icon advances through the available windows To advance to the next Time Window the current window s settings must have different start and end values Matching values indicates that the previous window is the final time window Start and end times are relative to the time you press the Run button to start operation The Computer icon indicates that a remote device is connect
96. sponse none 4 5 Foxy9 R1 and Foxy R2 Fraction Collectors Section 4 Serial Command Control 4 6 4 5 2 Immediate Control Commands Unsupported Miscellaneous Commands The following serial commands are not supported by the Foxy R1 and R2 fraction collector e DISPLAY e KEY keys These commands were available for earlier fraction collector models Do not use them with the Foxy R1 and R2 fraction col lectors COLLECT The unit resumes collecting fractions after a DRAIN command if this command is given during a run Oth erwise this command will instruct the fraction collector to move the drop former over the tubes and open the valve See also the DRAIN command Example Command COLLECT Response none DETECTOR The unit responds to this command with the current peak detector input value in volts Example Command DETECTOR Response DETECTOR 0 00014 DRAIN This command halts collecting If executed during a run it is the same as touching the Waste icon The three way valve diverts the flow to waste See also the COLLECT command Example Command DRAIN Response none The unit will re home itself to verify position even if it is already at home position Example Command HOME Response none NEXTTUBE The unit will advance the current tube number one tube Example Command NEXTTUBE Response none F oxy9 and R2 Fraction Collectors Section 4 Serial Command Contr
97. t Output RX TX auto MDI MDIX Termination Termination 1 8 Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction Table 1 5 Pump Connector Pins Connector in Description Input Count pump volume signal Output Pump pause stops pump between tubes and at end of run Output Valve output control signal for optional high flow diverter valve Common Circuit common Output 24 VDC Output power for optional high flow diverter valve Not used Shield Earth ground a Input is 3 3 to 5 VDC or contact closure active low 30 ms minimum 5 VDC maximum b Output is open collector active low 15 VDC 100 mA maximum Table 1 6 Detector Connector Pins Connector Pin Type Description Common Analog common for use with pin 8 Input Ext peak Not used Output Event pulse delivered at tube change Input Inject starts an inject cycle1 Input Tube Advance1 Common Circuit common Input Analog detector signal used for peak detection Shield Earth ground a Input is 3 3 to 5 VDC or contact closure active low 30 ms minimum 5 VDC maximum b 300 millisecond output is open collector active low 15 VDC 100 mA maximum c Analog input is 0 to 1 0 V nominal 1 2 to 1 2 V usable 5 0 to 5 0 V maximum 1 9 Foxy9 R1 and Foxy R2 Fraction Collectors Section 1 Introduction Table 1 7 RS 232 Serial Communication Con
98. tact sco Service Department 2 This list is subject to change without notice B 11 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix Replacement Parts Listings 603812182 REPLACEMENT PARTS LIST SHEET 11 OF TELEDYNE ISCO FOXY RI REV i PART NUMBER DESCRIPTION 5 233010400 4 F LAT WASHER 603875053 DISPLAY RIBBON GABLE J9 603875052 GPU CBA TO CO CBA RIBBON CABLE 54 692134164 ARM OPTICAL SWITCH ix 231514306 4 40 X 3 8 PANHEAD SCREW 56 602133666 A ARM IDLER PULLEY 5l 602134413 DISPLAY W BE ASSEMBLY 58 6038 4095 A ARM DRIVE BE W SPLICE 59 48900000 CABLE CLIP W ADH BACK NOT SHOWN 60 02901140 ad X 7 16 VINYL IBG NOT SHOWN 6 023050204 03 ID X 062 OD TBG NOT SHOWN 6 602134115 ADJUSTABLE FOOT ASSEMBLY 63 231514308 4 40 X 1 2 PAN HEAD SCREW NOTE 2 For current prices and quotations on parts contact sco Service Department This list is subject to change without notice B 12 Foxy9 R1 and Foxy R2 Fraction Collectors Appendix B Replacement Parts Listings ea O Q Ov Ti PAN 1 N NS i
99. tage 3 15 Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming To program the fraction collector for Threshold peak detection Figure 3 14 Threshold Settings menu 3 16 1 Touch the Threshold Detection icon from the Method Set tings menu to open the threshold menu Figure 3 14 eoa lt gt VA Enter the threshold as 1 to 99 percent using the Arrow icons The Up and Down Arrows increment or decrement the numbers the Left and Right Arrows move the cursor The icon next to the percentage shows what the fraction collector will do with non peak fluids The icon that is clear below the threshold indicates that non peak fluids will be diverted to waste The icon that is shaded below the threshold indicates that non peak fluids will be collected in tubes Touch the icon to toggle the selection Threshold peak detection can be used alone or combined with slope detection You can control these options using the icon in the upper right corner of the display Axe The None icon left indicates that Threshold detection is Off The Intersection Venn diagram icon middle indicates that both Threshold and Slope detection must be true before the fraction collector isolates the peak This is also considered a logical AND The Union Venn diagram icon right indicates that when either Threshold or Slope detection are true fraction collector isolates the peak This is also considered a lo
100. the Waste port Route the headless nuts of the In and Waste fluid lines down through the tubing guide on the back of the arm The tubing should be routed behind the metal guide pin Connect the fluid In line to the center port Connect the other end to the liquid source with user supplied fittings Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 11 Connect the Waste line to the right side port when viewed from the back of the diverter valve Route the other end to a waste collection container The completed connections should appear as shown in Figure 2 8 Figure 2 8 Connections to standard diverter valve 12 Raise the diverter valve to its highest position 13 Move the arm and diverter valve through the full range of motion Ensure the In and Waste tubing do not obstruct the movement If necessary secure the tubing using the adhesive backed clips from the accessory package 2 2 2 High Flow Diverter Valve Connections Figure 2 9 shows the high flow diverter valve ports To connect the fluid lines 1 Place the unit in Standby by touching the Standby ON icon 2 Remove any installed racks 3 The accessory package includes a 42 inch 107 cm length for the In port and the source The accessory package also includes an 8 ft 2 4 m length of tubing for the Waste port Cut each tubing piece to the desired length 2 7 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use
101. the threshold value and a slope is determined by the peak width setting Union OR The fraction collector cuts peaks 1 through 4 wherever the input signal exceeds the threshold value or a slope is determined by the peak width setting Isolated Peaks grey Isolated Peaks grey 3 19 Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming 3 3 7 Time Windows The Time Windows function allows the selection of up to four durations of the running time of which the fraction collector should enable collection time window may be used for several applications For example one can be used to reject peaks of no interest to cut peaks to increase the concentration of the sample or to divert initial waste volume An example of this type of collection is shown in Figure 3 20 In this figure two time windows are combined with threshold peak detection The fraction collector diverts the initial tubing volume and a peak of no interest 1 to waste until the start of Time Window 1 The fraction collector continues to divert fluid to waste until it detects Peak 2 at which time it begins collection until the end of the peak Peak 3 although it exceeds the threshold is diverted to waste because it does not occur in a time window Without a detected peak at the start of Time Window 2 fluid is still diverted to waste until the start of Peak 4 This peak is collected At the end of Peak 4 the fraction collecto
102. the diverter valve is in the highest position 8 Move the arm and diverter valve through the full range of motion Ensure the In and Waste tubing do not obstruct the movement If necessary secure the tubing using the adhesive backed clips from the accessory package To Waste Figure 2 11 Connections to high flow diverter valve 2 2 3 Drip Tray Drain Connect the 9 16 inch I D clear vinyl tubing to the drip tray drain Connection and route it to a suitable waste container N CAUTION Connect tubing to the drip tray drain when collecting large vol umes when the installed location is at risk from any overflow or when collecting fluids that are flammable or can produce flammable vapors CAUTION To prevent fluid from pooling drain tubing must slope down ward from the drip tray without loops or kinks If necessary ele vate the fraction collector by placing it on a sturdy shelf or platform 2 9 Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 2 3 Rack Installation 2 10 N CAUTION Visually inspect the drain tubing monthly to ensure that it is not obstructed The tubing should be free of any damage kinks deterioration or obstructions The fraction collectors hold either one or two racks Racks ensure that the collection vessels are positioned correctly under the drop former Racks are also a convenient way to prepare the fraction collector for operation as well as carrying or s
103. this applies to your application racks must be installed correctly on the guide pins to ground the collection vessels An electrical ground is necessary to dissipate electro static charges that may build while collecting fluids such as organic solvents 5 Verify that the spring loaded post on the left side of the rack is touching the guide pin Figure 2 13 Spring loaded Ground Post Guide Pin VAUVA Figure 2 13 Rack installed with ground post touching guide pin Foxy R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use 2 4 Diverter Valve Adjustment The height of the diverter valve should be adjusted to minimize the distance above the collection tubes This vertical adjustment should be performed whenever different types of racks or collection tubes are installed Z CAUTION To prevent damage to the arm drop former or collection tubes always adjust the diverter valve to its highest position before installing different types of racks or tubes After installing racks and tubes lower the diverter valve to its operating position Ensure that the unit is in Standby If not touch the Standby On icon to place the unit in Standby Move the arm and drop former to a convenient location over the installed collection rack While holding the diverter valve with one hand loosen the thumb screw s with the other Do not remove the thumb screw s Low
104. toring collected fluids after operation M Note To prevent spills always insert the correct vessels in the rack and verify that the rack is aligned on the guide pins M Note The Foxy R2 fraction collectors will detect the installed rack using RFID technology and configure the operation settings for the detected rack and tube size To install a rack 1 Select the rack and insert the collection tubes M Note Collection tube height must not exceed 150 mm for use with the Foxy R1 fraction collector Tube height must not exceed 180 mm for use with the Foxy R2 fraction collector 2 Ensure that the tops of the collection tubes are even Tubes that do not drop fully into the rack might obstruct arm movement 3 Grasp the rack so that tube 1 is positioned at the front left corner 4 Align the holes on the left side of the rack with the corre sponding guide pins Figure 2 12 in the drip tray and gen tly press downward When correctly installed both sides of the rack will sit on the ridges in the drip tray The test tube numbers on the rack will appear upright when viewed from the front of the fraction collector Foxy9 R1 and Foxy R2 Fraction Collectors Section 2 Preparation for Use Guide Pins Figure 2 12 Rack guide pins and bottle location ridges N CAUTION Risk of fire or explosion Nonconductive nonpolar liquids at lin ear velocities greater than 40 cm sec will develop an electro static charge If
105. tougher stains Alternatively the tube can be placed in an ultrasonic bath CAUTION Use care to avoid scratching or otherwise damaging the glass tube 5 Place the cleaned glass sleeve back into the retaining ring and thread the ring back into the diverter valve 6 Replace the collection racks connect the AC mains power cord and return the fraction collector to operation Z CAUTION Operating the fraction collector without the glass sleeve may damage electronic components inside the diverter valve assembly Never operate the fraction collector without the glass sleeve F oxy9 R1 and R2 Fraction Collectors Section 5 Maintenance 5 3 Service 5 4 Instrument Returns 5 4 5 4 1 How to Ship Returns WARNING Risk of serious personal injury or equipment damage The fraction collector does not contain user serviceable parts Service panels and cover should only be removed by trained personnel Service problems often can be resolved by telephone or E mail US customers can receive support by contacting the factory Tech nical Service department Contact information can be found on the Foreword and Warranty pages at the front and back of this manual If you contact the factory by E mail please include the serial number and all relevant information regarding the diffi culty you are experiencing with the instrument Customers outside of the US should contact their Teledyne Isco dealer for service suppo
106. ure 3 4 Restart 1 The fraction collector restarts the col lection program and moves to the tube defined by the overlay next or skip options This process repeats until the end of the run Timed The timed options do not rely on external signals nor does it pause for injections When using one of the timed options the fraction collector assumes that each of the multiple sample injections are performed at fixed time intervals For example the chemist injects a sample and touches the Play icon to start the run Figure 3 5 Thereafter another sample is injected at every five minute interval Every five minutes the fraction collector automatically restarts and moves to the tube defined by the overlay next or skip options This process repeats until the end of the run Samples Se SS SS Start of Run 8 Be art mr Figure 3 5 Timed options timing diagram 3 7 Foxy R1 Foxy R2 Fraction Collectors Section 3 Programming Overlay Next or Ship Overlay Next Skip To set the time restart interval 1 Touch the Arrow buttons to select a Timed option A Folder icon appears at the top of the display 2 Touch the Folder icon to open the Timed interval settings 3 Enter the hours minutes and seconds for the interval Move the cursor position by touching the Left and Right Arrow icons Touch the Up and Down Arrow icons to set the numerical value at the cursor position 4 Touch t
107. us practices If you use this instrument in any way not specified in this manual the protection provided by the instrument may be impaired WARNING If this system uses flammable organic solvents Teledyne Isco recommends that you place this system in a well ventilated environment designed for these types of materials This environment should be constructed in accordance with federal state and local regulations It should also comply with your organization s plan concerning chemical and hygiene mishaps In all cases use good laboratory practices and standard safety procedures WARNING For indoor use only N WARNING If you are using flammable solvents or chemicals with this system vapor concentration levels may exceed the maximum exposure levels as recommended by OSHA Guide 1910 1000 To reduce those levels to a safe exposure Teledyne Isco recommends that you place the system in a laboratory hood designed for the purpose of ventilation This hood should be constructed and operated in accordance with federal state and local regulations In the event of a solvent or chemical spill your organization should have a plan to deal with these mishaps In all cases use good laboratory practices and standard safety procedures x Foxy9 R1 and Foxy R2 Fraction Collectors Safety Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts These three levels are described in the sample alerts below
108. utomatically detect the rack type without user intervention to set collection patterns and vessel capacities The Foxy R2 fraction collector is also available with a high flow rate up to 1000 mL min diverter valve Figure 1 1 Foxy R1 left and Foxy R2 right fraction collectors 1 1 Foxy R1 Foxy R2 Fraction Collectors Section 1 Introduction 1 1 Collection Schemes The fraction collector supports several types of collection schemes Basic collection schemes collect uniform volumes frac tionation These uniform modes for tube advances include Drop Counting The fraction collector counts the drops from the drop former When the count reaches a user specified number of drops the fraction collector advances to the next tube The fraction collector can count up to 999 drops per fraction Drop counting is not available on the Foxy R2 High Flow fraction collector Time Intervals The fraction collector can advance to the next tube at fixed time intervals Tube changes can occur as rapidly as every second or wait up to 99 59 59 HH MM SS Volume Intervals The fraction collector can switch tubes at fixed volume intervals This collection mode requires an input signal from a pump that correlates its delivery vol ume This signal can represent actual volumetric units or more typically a pump revolution counter The fraction col lector can count up to 999 units The fraction collector can perform additional tube
109. ve the name and return to the Method Selection menu Foxy9 R1 and Foxy R2 Fraction Collectors Section Programming lt gt VA Figure 3 9 Rename Method display 3 3 2 Drop Counts Drop counting creates fractions by advancing the drop former at a fixed number of drops This option uses a photosensor device 4 positioned just below the drop former Drop counting can create relatively uniform collection tube vol umes even when the delivered flow rate varies However if the fluid viscosity varies while running a gradient the collection tube volumes may vary M Note The drop count option assumes that the flow rate is slow enough to produce drops instead of a stream of liquid Conse quently drop counting is not supported by the Foxy R2 High Flow fraction collector To create fractions based on drop counts 1 Touch the Drop Count icon to open the settings display Figure 3 10 Figure 3 10 Drop Counts display Foxy9 R1 and Foxy R2 Fraction Collectors Section 3 Programming Use this display to set the fraction volume expressed as a number of drops and the flow delay volume expressed as time 2 Ensure that the fraction volume setting is active as indi cated by the Volume Select icon That is the tube should be highlighted with a border If not touch the icon to toggle the setting ii 3 Enter the fraction volume as 1 to 999 drops using the arrow icons The Up and Down Arrows increment

Foxy R1 and Foxy R2 Fraction Collectors

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