Respiratory assessment of a canine model of XLMTM - Treat-NMD
Contents
1. 312 CURE CMD AFMTELETHON 8 CURETHROUGH INNOVATION a XLMTM_D 2 2 002 Please quote this SOP in your Methods Respiratory assessment of a canine model of XLMTM using non invasive respiratory impedence plethysmography SOP ID Number XLMTM_D 2 2 002 Version 1 Issued March 26 2014 Last reviewed March 26 2014 Author Melissa A Goddard University of Washington Seattle WA Working group Martin K Childers David L Mack members University of Washington Seattle WA SOP responsible Melissa A Goddard Official reviewer Martin K Childers Page 1 of 8 aW SVE CURE 0 AFMTELETHON O CURE THROUGH INNOVATION a XLMTM_D 2 2 002 TABLE OF CONTENTS OBJEC TIV Emaisia iinei EAA AEE a eee 3 SCOPE AND ien R EE OEO REEE RE EREET 3 CAUTIONS E ranana EOE OEE E emu EE AE E EAEE ARE E 3 MATERIALS tame ENE EEO E eoere 3 METHODS T EA E E RS 4 EVALUATION AND INTERPRETATION OF 8 REFERENCES 8 Page 2 of 8 2 0 2 CURE CMD AFMTELETHON 2 CURETHROUGH INNOVATION a XLMTM_D 2 2 002 1 OBJECTIVE Anesthetised dogs are fitted with wired bands and connected to a calibrated pneumotac2. the EKG leads as follows Green at the groin White on the right Black on the left Feed the band material through the loops of the inner jacket and place the inner jacket on the dog so that the bands face outwards The metal part of the bands should also be visible ie facing outwards Check to ensure that the thoracic and abdominal leads are in an appropriate open position feed the end of the band material into each and then close Ends should lock firmly but should not need to be forced as this may cause damage The blue measures the thoracic band The yellow measures the abdominal Dog should appear as follows Place the outer jacket the dog Optional Connect thoracic abdominal and EKG leads to the telemetry pack Connecting the EKG lead turns the unit on Ensure that a signal is being obtained on the computer Place the telemetry unit in the pouch of the outer jacket before continuing with any planned experiments Optional A catheter should also be placed in the fore or hindlimb for the injection of doxapram hydrochloride Page 7 of 8 S CURE CMD 6 AFMTELETHON 2 CURETHROUGH INNOVATION a XLMTM_D 2 2 002 Run the experiment and start streaming and storage O To start streaming select Start Stop gt Storage Streaming Click start streaming Check the Save beats box Click Ok Or click the pre progr
3. amed F1 key O oO To stop streaming press F2 To start storage select Start Stop gt Storage Streaming Click start storage Select the site or press Select All sites Click Ok Or click the pre programed F3 key To stop storage press F4 Doxapram hydrocloride administration Create a label in lox by pressing the F12 button and entering the amount of doxapram Administer a bolus injection of 1 0mg kg of doxapram hydrochloride followed rapidly by a saline flush Press Enter at the same time the dose is administered to label O Collect a minimum of 20 clear breaths once respiration has reached its peak Oo oO 6 EVALUATION AND INTERPRETATION OF RESULTS lox and ecgGAUTO version 2 8 2 6 EMKA Technologies calculates various respiratory parameters from the measured curves such as respiratory rate tidal volume peak inspiratory and expiratory flows and thoracic and abdominal volume The software can also be used to generate reports of these values including flow volume or phase curves 7 REFERENCES 2 user manual EMKA technologies V2 4 rev 2 2010 Goddard MA mitchell EL Smith BK Childers MK Establishing clinical end points of respiratory function in large animals for clinical translation Phys Med Rehabil Clin N Am 2012 23 1 75 94 xi Page 8 of 8
4. hometer at the time of assessment After a baseline ventilatory pattern is established and measured the respiratory stimulant doxapram hydrochloride is given Ventilatory parameters are assessed from measures of airflow and by impedence 2 plethysmography as a function of time in response to doxapram SCOPE AND APPLICABILITY Respiratory impedence plethysmography RIP and pneumotachography have been used successfully in larger animal models to measure respiration Similar non invasive systems have been applied clinically in patients with neuromuscular disorders to assess the respiratory dysfunction that is often present in these diseases 3 CAUTIONS The dog should not be shifted or moved during measurement as can cause noise or disruptions in the RIP signal MATERIALS EKG pads EMKA underjacket and outer jacket of an appropriate size typically the underjacket is one size smaller all come in small medium and large EMKA Technologies Alcohol Shaver Wired band material EMKA Technologies Scissors Abdominal thoracic and EKG leads EMKA Technologies Pneumotachometer EMKA Technologies Calibration unit and t r box EMKA Technologies Antennae EMKA Technologies Small screw driver included with calibration unit EMKA Technologies Laptop with iox2 software 2 8 0 13 Windows XP Professional service pack 2 or 3 Telemetry pack EMKA Technologies 1L super syringe EMKA Technologies Tubing AA Durace
5. ld load automatically as follows for O EKG O Act RIP Thor and Ab bands RIP sum channel u n O Uncalibrated inputs will show as their values in the calibration main screen under Last calibration date time If this occurs values can be set as follows Select 2 points manual calibration Enter the Low and High values Enter the unit of measure in Unit Low High Unit EKG 0 1 mV Act 0 1 G 5V 1 45V RIP 0 1000 m s 0 1V RIP Sum 0 1000 m s Click Ok Page 5 of 8 Wu lt CURE CMD 2 0 AFMTELETHON 2 CURETHROUGH INNOVATION a XLMTM_D 2 2 002 O Calibrate RF Calc the pneumotach signal Select Tuning gt Calibrate from the run menu Oo Select integrate calibration using the dial Enter the Low value 0 and the High value 1000 Enter the unit of measure in Unit ml sec or 1 O Set the calibrating unit amplifier to DC using the switch O Set the 1 Adjust the coarse adjustment lowest screw using the provided screw driver until the signal is close to OV no flow Adjust the gain in a stepwise fashion dialing back until the signal returns to 0 using the fine adjustment dial Set the integration period to the number of seconds sufficient to inject the volume 2s or 3s is ideal for accurate calibration although longer periods can be used initially Attach the 11 syri
6. ll Procell batteries Electrode gel optional Page 3 of 8 147 e lt CURE CMD CURE THROUGH INNOVATION a XLMTM_D 2 2 002 Software key lox EMKA Technologies Doxapram hydrochloride Saline Syringe Needle SD card 5 METHODS Equipment Set Up Ensure that equipment is properly connected and functioning T r box connections should be as follows when viewed at the back unconnected connected 1 lead 1 2 ECG lead 2 4 Activity 6 Thoracic band 7 Abdominal band Lights on the front of the T r unit should glow green Signal strength should be green or high yellow when telemetry box is on Connect the antenna Turn on the unit Connect SD box and insert into laptop Page 4 of 8 Wu lt CURE CMD 2 0 AFMTELETHON P CURETHROUGH INNOVATION a XLMTM_D 2 2 002 Load saved config file Insert the software key and open the lox Software Load saved configuration file created by installing technician Define subject names Optional Select Run from the main menu to display subject name Input the name or number of the test animal with a unique modification so there is no duplication in the data directory Click Run Select Tuning gt Calibrate Calibrate the inputs Calibration values shou
7. nge to the pneumotachometer using the tubing When connected correctly pushing air in should cause a negative deflection to be measured C Flip the metal transducer if this is not observed and reconnect the pneumotach Inject the volume and immediately click the High button Ensure that the signal is within range and that injection time does not L exceed the integration period Area under the curve should be between the 3s O If not dial up the gain until the scale fits Switch the calibration unit back to AC and deselect remove AC offset on the program O Click Ok Save this new configuration file under a new name which includes the date of creation Preparation of Dogs and Jacket Fitting Shave the anaesthetised dog as cleanly as possible in the appropriate areas O under the left armpit of the forelegs O under the right armpit of the forelegs On the left side of the groin Size the dog for the thoracic and abdominal bands in such a way that they fit snuggly but still allow two fingers under the band Cut using the scissors Page 6 of 8 al lt CURE CMD 2 AFMTELETHON CURE THROUGH INNOVATION a XLMTM_D 2 2 002 Wipe the area with alcohol to remove any stray hairs and fix the EKG pads firmly to the dog Electrode gel can also be used to improve adhesion and conductivity Connect
Download Pdf Manuals
Related Search