EXT-10C - NPI Electronic Instruments
Contents
1. A amp Bo th M 2010 Field potential signature of distinct multicellular activity patterns in the m ouse hippocampus J Neurosci 30 15441 15449 Y Ogden D ed 1992 Micr oelectrode Techniques The P lymouth Workshop Handbook Second Edition The Company of Biologists Ltd Cambridge I Seidenbecher T and H C Pape 2001 Contri bution of intralam inar thalamic nuclei to spike and wave discharges during spontaneous seizures in a genetic rat model of absence epilepsy European Journal of Neuroscience Vol 13 1537 1546 d Weiss E K Krupka N Bahner F Bot h M amp Draguhn A 2008 Fast effects of glucocorticoids on m emory related network oscillations in the mouse hippocam pus J Neuroendocrinol 20 549 557 Ld Werdin F Grussinger H Jam inet P Kraus A Manoli T Danker T Guenther E Haerlec M Schaller H E amp Sinis N 2009 An improved electrophysiological method to study peripheral nerve regeneration in rats J Neurosci Methods 182 71 77 LJ Windhorst U and H Johansson eds 1999 Modern Techniques in Neuroscience Research Springer Berlin Heidelberg New York version 2 0 page 11 Technical Data Differential Input Input resistance Output DC Output AC Offset compensation Potential monitor Capacity compensation Size Headstage Size Holding Bar EPMS 07 SYSTEM Power Requirements Dimensions version 2 0 EXT 10C User Manual CMR g2. D Electronic f Instruments for the Life Sciences poAsube ade Te OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE EXT 10C EXTRACELLULAR AMPLIFIER MODULE FOR EPMS SYSTEMS VERSION 2 0 npi 2012 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com EXT 10C User Manual Table of Contents Ise BeBe by lA a ease eE O o A 3 2 ERMS 07 Modular Plus SSA a A EE ease ee 4 213 Components o1 the EPWIS207 HOUSING ta 4 2 2 General System Description Operation ccceeeesssseeeeccccceeeeeeeeeesaaaaassesseseeeeeeeeees 4 DO EMS EU dE 4 2A NO O ING dice ie can etalk etude a a h see Adu O 5 233 A AA A ales tt o nn Gulw aie E oir 5 A hada tote gacea tad esters fame tale E Ae asctsstiash donc oun oe 6 JL ATL OO COMPONE SS A Ai 6 z2r DeM TOC SCRIP OM doit 6 Si DISMAL Oy Diaa raie a E eaten snes atau eee uae 6 JA Descuptuon o THe FLOM nl aia 7 R A e oa A are ae ee ae 9 UN AAA A A 9 320 UPON id ditds 9 d AA cid 11 Technical Data aa 12 version 2 0 page 2 EXT 10C User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic mu
3. T 2 009 Distinct Firing Patterns of Identified Basket and Dendrite Targeting Interneurons in the Prefrontal Cort ex during Hippocampal Theta and Local Spindle Oscillations Journal of Neuroscience 29 9563 9574 I Huemmeke M Eysel U T amp Mittm ann T 2002 Metabotrop ic glutamate receptors mediate expression of LTP in slices of rat visual cortex Eur J Neurosci 15 1641 1645 I Huemmeke M Eysel U T amp Mittm ann T 2004 Lesion indu ced enhancement of LTP in rat visual co rtex is mediated by NMDA receptors containing the NR2B subunit J Physiol 559 875 882 LJ Schulz D Huston J P Jezek K Haas H L Roth Harer A Selbach O amp Luhmann H J 2002 Water maze performance exp loratory activity inhibitory avoidance and hippocampal plasticity in aged s uperior and inferior learners Eur J Neurosci 16 2175 2185 I Kettenmann H amp Grantyn R eds 1992 Practical Electrophysiological Methods Wiley Liss New York d Lalley P M A K Moschovakis and U Windhorst 1999 Electrical Activity of Individual Neurons In Situ Extra and Intracellular Recording in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin New York LJ Muller Ch M 1992 E xtra and Intracellular Recording in the Slice in Kettenm ann H amp Grantyn R eds Practical Electro physiological Methods Wiley Liss New York d Reichinnek S Kunsting T Draguhn
4. aciti es of the m icroelectrode 10 turn potentiometer range 0 20 pF Due to the positive feedback circuit overcompensation of the capacity will lead to oscillations which may damage the cell Mal The analog POTENTIAL monitor displays the offset in the range of 30 mV E BW and is used for optimal cancellation of the OFFSET 6 OVER OVER LEDs PE LEDs that indica te if the am plifier 10 below it s positive or negative lim it 10 V The linear range of the amplifier is 12 V 7 OFFSET potentiometer 10 turn potentiometer to compensate for the OFFSET of a DC signal Note Position 5 on the OFFSET control corresponds to 0 mV offset 8 GAIN AC switch Switch to set the GAIN of the outgoing AC signal Three GAIN factors are available x100 x1k or x10k 9 AC OUTPUT connector 4 BNC connector providing the am plified signal AC coupled The a mplification factor is set by switch 8 OUTPUT 10 NOTCH switch optional Switch to set the NOTCH filter 50 Hz 60 Hz or to bypass the NOTCH filter switch position OFF for AC measurements version 2 0 page 8 EXT 10C User Manual 3 5 Headstage AMPLIFIER HEADSTAGE TYPE j EX O O at AI Figure 4 electrode holder optional and headstage of the EXT 10 2F Headstage Elements Pet BNC connector for the electrode holder measuring electrode REF SMB connector for the reference electrode GND Ground connector Holding bar
5. eadstage see Figure 5 version 2 0 page 9 EXT 10C User Manual If differential measurement is not required single ended measurement configuration see Figure 5 the REF inp ut must be connected to ground GND The amplifier is in an undefined state if the REF is left open an d can go into saturationm aking reliable measurements impossible A B Experimental Experimental chamber Slice chamber Slice Ag AgC pellet REF AGAJ ellet electrode ps Electrode noder BNC BNC jack jack Q Pa 9 E Q U D 5 a C E GND REF REF Ground SUBCLICK SUBCLICK jack connector jack connector REF REF Cable to Cable to amplifier amplifier differential single ended measurement measurement configuration configuration Figure 5 headstage connections A differential measurement B single ended measurement version 2 0 page 10 EXT 10C User Manual 4 Literature 1 Barmashenko G Eysel U T amp Mittm ann T 2003 Changes in intracellular calcium transients and LTP in the surround of visual cortex lesions in rats Brain Res 990 120 128 _J Boulton A A Baker G B amp Va nderwolf C H eds 1990 Neurophysiological Techniques Basic Methods and Concepts Humana Press Clifton New Jersey Ld Both M Bahner F Bohlen Und H O amp Draguhn A 2008 Propagation of specific network patterns through the mouse hippocampus Hippocampus Y Hartwich K Pollak T amp Klausberger
6. im WN On request P gp can beim plemented using 1mm or 2 mm banana jacks or using SMB connectors Very Important EXT 10C headstages are always labeled EXT see Figure 4 and must not be exchanged with headstages from npi electronic s desktop EXT amplifiers e g the EXT 2F which is labeled EXT 02 Also Important The shield of the BNC connector of the headstage is connected to driven shield and must not be connected to ground 3 6 Operation Extracellular measurements are mostly done in slices or in vivo where distortions of the signal caused by other instrum ents and the animal itself are very co mmon Additionally extracellular signals are very sm all and have to be amplified enormously The drawback is that noise is amplified as well Therefore the headstage of the EXT 10C is equipped with a differential input that m inimizes noise pick up Differential means that the signal for the amplifier is the difference between the positive PL and negative REF input of the headstage This results in canceling of all signals which both electrodes record e g noise For differential measurements both inputs of the headstage REF and P gL are connected to microelectrodes using cables with grounde d enclosure or electrode holders P gy is connected to the measuring electrode and REF to the re ference electrode The experimental chamber is grounded by an Ag AgCl pellet or an AGAR br idge connected to G ND of the h
7. measurements The EXT 10C is designed to be used with the DPA 2F preamplifier filter module from npi which combines additional gain stages with high and lowpass filters 3 3 Signal Flow Diagram The signal is passed through the EXT 10C as shown below once oc Eiichi m a m D e ji a e CAUTRLIT A oe Core Maca A am we g Catal T B tir i id e ba e o m an MPUT Mosca Onoto LEPE CAA TIA AS z P version 2 0 page 6 EXT 10C User Manual 3 4 Description of the Front Panel gt ExT 100 over O O OVER POTENTIAL x10mY a O HEADSTAGE Figure 3 EXT 10C front panel view In the following description of the front panel elements each element has a number tha t is related to that in Figure 3 The number is followed by th e name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element is given 1 HEADSTAGE connector The HEADSTAGE cable is connected to the unit at an 8 pin connector in the center of the module BNC connector providing the am plified signal DC coupled The a mplification factor is set by the GAIN DC switch 3 The DC OUTPUT is always unfiltered version 2 0 page 7 EXT 10C User Manual 3 GAIN DC switch Switch to set the GAIN of the outgoing DC signal Three GAIN factors are available x10 x100 or x1k 4 C COMP potentiometer Control for com pensation of stray cap
8. power supply unit the power switch and the fuse are located at the rear of the housing 2 3 EPMS E 07 Housing The EPMS E 07 housing is designed for low noi se operation especially for extracellular amplifiers and filters which are plugged in It operates with an exte rnal power su pply to minimize distortions of the signals caused by the power supply The external power supply PWR 03D supports up to 3 EPMS E housings and each housing is connected to the rear panel of the EPMS E 07 by means of an 6 pole cable An LED indicates that the PWR 03D is powered on P ower switch voltage selector and fuse are located at the rear panel Figure 1 PWR 03D front panel view version 2 0 page 4 EXT 10C User Manual Figure 2 PWR 03D rear panel view Note This power supply is intended to be used with np1 EPMS E systems only 2 4 System Grounding The 19 cabinet is grounded by the power ca ble through the ground pin ofthem ains connector protective earth In order to avoid ground loops th e internal ground is isolated from the protective earth The internal gr ound is used on the BNC connectors or G ROUND plugs of the m odules that are inserted in to the EPMS 07 housing The internal ground and mains ground protective earth can be conne cted by a wire using the ground plugs on the rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connec
9. st be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is design ed for use in scientific laboratories and m ust be operated only by trained staff General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling el ectronic devices Before using any device please read manuals and instructions carefully The device 1s to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a th ree wire line cord and am ains power plug with a p rotection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fu se or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipm ent is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a gr ounded metal surface when changing or adjusting sensors Always turn power off
10. t 90 dB at 1 kHz tested with 0 Q input resistance gt 10 Q range 1 V selectable gain x10 x100 x1k Output range 12 V into 1 KQ 1 V into 50 Q load selectable gain x100 x1k x10k Output range 12 V into 1 kQ 1 V into 50 Q load corner frequency 10 Hz NOTCH filter 50 60 Hz optional 10 turn potentiometer 100 mV analog display for the electrode offset range 30 mV 0 20 pF 10 turn potentiometer front panel 12 HP 60 6 mm x 3U 128 5 mm 7 175 mm deep 70 x 26 x 26 mm length 150 mm diameter 8 mm 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W dependent on the modules plugged in 19 rackmount cabinet 3U high 1U 1 3 4 44 45 mm page 12
11. ted We recommend that you try both arrangements to determine the best configuration 2 5 Technical Data 19 rackmount cabinet for up to 7 plug in units Power supply 115 230 Volts AC 60 50 Hz fuse 2 A 1 A slow 45 60 W External power supply for EPMS E 115 230 Volts AC 60 50 Hz fuse 1 6 0 8 A slow Dimensions 3U high 1U 1 3 4 44 45 mm 254 mm deep with high voltage power supply 354 mm deep version 2 0 page 5 EXT 10C User Manual 3 EXT 10C 3 1 EXT 10C Components The following items are shipped with the EXT 10C system Amplifier module for the EPMS 07 system GND 2 6 mm banana plug and REF SMB connectors for headstage Headstage User manual SSSS 3 2 System Description The EXT 10C was designed for perform ing low noise recordings of small extracellular signals in slices or in in vivo preparations using fine tipped glass or metal microelectrodes The system consists of a module for the npi EPMS 07 modular system and a small headstage with a holding bar The EXT 10C has a differential input with high input resistance for minimizing noise and the incoming signal can be compensated for offset and capacity of the microelectrode by the two potentiometers OFFSET and C COMP An anal og balance m onitor makes the control of offsets easy The recorded signal can be obtai ned either AC with GAIN of 100 1k or 10k or DC with GAIN of 10 100 or 1k An optional NOTCH filter eliminates 50 60 Hz noise in AC
12. when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT W ARM UP TIME All analog electron ic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal tem perature has reached steady state values In most cas es a war m up period of 20 30 m _ inutes is sufficient HANDLING Please p rotect the device from moisture h eat radiation and corrosive chemicals version 2 0 page 3 EXT 10C User Manual 2 EPMS 07 Modular Plug In System 2 1 Components of the EPMS 07 Housing The following items are shipped with the EPMS 07 housing EPMS 07 cabinet Power cord Fuse 2 A 1 A slow Front covers AANA 2 2 General System Description Operation The npi EPMS 07 1s a m odular system for processing ofbioe lectrical signals in electrophysiology The system is housed in a 19 rackmount cabinet 3U containing a power supply and has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units m ust be kept in position by four screws M 2 5 x 10 The screws are important not only form echanical stability but also for prope r electrical connection to the system housing Free area must be protected with covers In order to avoid induction of electrom agnetic noise the
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