416 User`s Manual
Contents
1. TCT 15 15 m conical 50 m conical 50 Q None NE GAS u15 50 GAS c50 50 173x897 295x175 La TYROZEN Dual 15 Bucket GLA d15 50 Bucket capacity 2 ea Bucket bore Ox L mm 217x915 Radius mm 151 5 Max RPM 4 000 Max RCF g force 2 700 Max height for tube fit mm 120 Tube Tube capacity m0 Bucket capacity ea 4 Low Speed Centrifuge 416 25 Swing Rotor GR5 5 mw 2 loadings MAI Area dimension mm 86 3 X 130 3 Max height for microplate fit mm 35 Plate Platecapacity m0 MTP Rotor capacity ea 2 2 4 Radius mm 111 Max RPM 4 000 Max RCF g force 1 986 Angle Rotor GRA 5 15 16 16 x 15 md 45 Hole diameter mm 18 Max height for tube Fit mm 130 e m E E i Tube Tubecapacityind 3 4 5 wo 15 15 mi conical Adaptor None None None Stainless steel 15 M2 sleeves MEA Cato 64531159 BEA GAS 5 15 rare an Adaptorbore xL mm 135x61 135x65 135x85 Max RPM 4 000 Max RPM i 4000 Max RCFIg force 2 100 Max RCFIg force 2397 2 433 2 692 2 638 2 700 2 700 3 26 Li LIZEN Low Speed Centrifuge 416 9 Product Range 27 La TYROZEN Low Speed Centrifuge 416 Doc No STC A08 113 KEL08 C10032 EC Declaration of Conformity We Gyrozen Co Ltd B Station 544 1 Bongmy2. ii eee 11 PPT Ar 11 Rotor coupling and disassembling a 12 POSON O AAA 14 ON 15 Key Functions of Control Panel a 15 ETT eae iii 15 Setting ITS 16 Ad i i i ii 17 SOFT Start StOp a 17 I see A 18 Bari dazae DOO eleiza aA 19 FUSE TSOIGGOTOOT a 19 VELIA AA 20 Outer part Of instrument a 20 TT iii 20 VNR 20 4 Li LIZEN Low Speed Centrifuge 416 6 4 Oo es 20 6 5 Transportaion of the IDSETUTDOT eee 20 7 Trouble Shooting a 21 7 1 cd EE OOO 21 7 2 Error COOG a 22 8 MT RI 24 9 Product Range a 27 10 CE III 28 b 5 Li LIZEN Low Speed Centrifuge 416 1 Meanings of Symbols amp Safety Precautions 1 1 Meanings of Symbols 1 1 1 Symbols on the device Symbol Meaning Symbol Meaning Attention and warning Attention and warning for electric shock l l Attention and warning CAUTION BUEE Rotori Attention and warning 00018 9 ML EEA 244 FAQ i e d i Please fix the rotor for rotor cou p lin g not to get hands caught firmly on place in the instrument closin g for door opening and Attention and warning l Attention and warning for correct way of for correct way of sample balancing in the 7 buckets position rotor Operate after mounting all of 4ea buckets Indicate a hole for manual door opening in case of emergency 1 1 2 Symbols in this document This symbol refers to safety Note A relevant warnings and indicates This symbol refers to the important possible dangerous outco
3. physical impact or turbulence 2 Do not mount a rotor in any cases of movement Fill inside the chamber with proper materials to keep the motor shaft on place and not to be influenced by physical pressure b 20 Liv LIZEN Low Speed Centrifuge 416 7 Trouble Shooting 7 1 Check list Symptom Check list Connect the AC Power cord and make sure that the line is completely Power failure connected between the instrument and power outlet Check the power switch is turned on Please refer to 4 1 Power On off and Door Release If the door is not closed completely the instrument can t run Can t be started Check the Door LED on the display window and close the door completely If the power is out check the main fuse for the laboratory to supply the power If it is not solved in shortly open the door with the Emergency Can t open the door Door Open Tool manually for safety of sample Please refer to 5 7 Emergency Door Open Remove the dirt at the door latch and then close the door completely Can t close the door again If the door seems not being closed by mechanical reason please contact our service team Please check the balanced status of both the table and the instrument Please re check the coupling status of the following three matches to minimize the noise the balanced way of coupling of the rotor into the motor shaft Noise and vibration during the completeness of fixing of the Rotor Locking Nut on t
4. Speed Centrifuge 416 5 Operation 5 1 Key Functions of Control Panel Setting Speed Setting Time Conducting Function Setting Speed RPM RCF For automatic conversion of RPM RCF and to set the speed Setting Time TIME Use to set time available range up to 99 min 0 00 00 continuous Setting Function PROG Use to save a set of setting values or recall the saved program number START STOP Use to start or stop operation Door Use to open the instrument lid Soft Start Stop For gentle acceleration and deceleration Forced only by natural gravity force 5 2 Setting RPM RCF i naro JIN J Maximum RPM RCF 4 000 RPM 2 700 x g Action 5 2 1 Setting RPM gt Speed setting unit 10 rpm or 100 rpm HARM HHH 1 Press a RPM RCF button once A D gt RPM MODE is generated by pressing a RPM RCF FR 15 Li LIZEN Low Speed Centrifuge 416 button once 2 Press the A V buttons to change input value gt After keeping holding finger on the A V buttonsfor 5 seconds the unit of setting value is changed to rom from 10rpm 3 Press the RPM RCF button again for saving NIA EA ZA NI FIT 00 II I 5 2 2 Setting RCF HHHHJg gt Speed setting unit 1 rcf or 10 rcf RPM C RCF A 1 Press a RPM RCF button twice gt RCF MODE is generated by pressing a RPM RCF Button twice e 2 Press the A V buttons to change input value gt After ke
5. 15 ml conical 15 ml conical 50 50 ml conical 85 100 Bioseal Bucket with O ring T T i EO er Bioseal 100 m Bucket ed bei be SQ GL8 b100 100 Tube rack Adaptor CD RD Bucket bore Ox L mm AILES WY YS QH7 bed VY bed GY zt A rn sn Zr EEE GAM m2 0 6 GAM 7 5 GAM 10 5 GAM 15 3 GAM c15 3 GAG CIS GAS SO GAS 50 eases GAS 100 pe der b100 b100 DOU 100 100 b100 b100 b100 b100 b100 herlei ie ZE 624 a 5 20 3 12 n 1 1 4 1 4 1 4 1 4 Max height for tube fit mm Rack capacitylea 4 lt 114 w cap 130 w o cap Hole dimension DxL mm 11x39 13 5x60 16x60 175x105 17 2x106 5 17 2x106 5 295x959 30x100 305x964 44x 1064 Vacuutainer Bucket b100 adaptors work fine with bioseal capping but 100 adaptors GLB 10 8 100 Bucket capacity B ea Radius mm 155 1 Max RPM 4 000 Vacuutainer 10 m Bucket Bucket bore Ox L mm 16x89 Max RCF g force 2 774 Max height for tube fitfmm 120 Tube Tube capacity m0 Bucket capacity ea 4 8 32 La TYROZEN Low Speed Centrifuge 416 24 Swing Rotor GRS 5 50 4 4 loadings b DI Round 50 m Bucket 6LB 50 50 Bucket bore Ox L mm 30 5x91 Radius mm 151 5 Max RPM 4 000 Max RCF g force 2 700 Max height for tube fit mm 125 Tube Tube capacity m Adaptor Cat No Adaptor bore O mm
6. Low Speed Centrifuge 416 User s Manual 18 arar ai N ITT an Gyrozen Co Ltd B station 30 12 Gyeryong ro 141 Yuseong gu Daejeon 305 301 Korea Tel 82 42 719 8200 Fax 82 42 826 9848 WWW gyrozen com DOC No C01DC00204 11 NE Ea e Et Designed to suit your exact needs Wide range of modern centrifuges for a wide variety of laboratory applications THE BEST FUNCTIONAL QUALITY Manufactured and tested to IEL standards stable spinning operation within 2 variation fa Steady and soft deceleration with dynamic brake technology bezi j m Low noise level with unique internal air flow design that protects heating of samples f m Automatic recognition and alarms for imbalance over speed and over heat x Automatic RPM RCF conversion SAFETY and ROBUSTNESS E Double laminated ABS Steel door construction minimizes noise and heat transmissions Safety door lock mechanism ensures the door is locked whilst in operation mode Unique door drop protection protects the operator and samples when loading and unloading Automatic detection and alarms for imbalance excess speed and heating Automatic door open scheme with safety level of aperture depth not to damage operators Emergency door lock release helps to open the instrument when power blackout oi sudden stoppage occurs m he Aerosol tight buckets and rotors prevent contamination and ensures safety Autoclavable and corrosion resistant rotors ensure safe
7. SOFT Start Stop The function of SOFT Start Stop button is gentle acceleration and deceleration for the sensitive samples Action d o 1 After setting Time and RPM press the SOFT Start Stop button once E gt The LED of SOFT Start Stop button is turned on b during running gt The Door is opened automatically when the instrument is completely finished gt When the instrument is running with this function it is not possible to stop running by pressing normal START STOP button 17 La TYROZEN Low Speed Centrifuge 416 5 6 Program Saving amp Recalling Action 5 6 1 Saving 1 Set parameters Refer to 5 2 5 3 F SR b EA Press the PROG button longer than 3 seconds to save your 1000 o preferred set values o gt The LED of PROG button and SEC Save are turned on C FN Eg 3 Input the program number by using A VW button A gt O Y gt Save up to 10 programs e C F 4 Press the PROG button again to complete the saving j N gt The setting value is saved gt If you do not press the PROG buttons for 5 second the Proc pa J setting mode is cleared n 5 6 2 Recalling 1 To recall the saved program just press the PROG button shortly gt The LED of PROG and MIN Call are turned on 2 Check the program number to call and enter the program number EO ence you want to recall by pr
8. ck it with spinning manually Do not stop the rotor by touching with hand during the instrument is running Emergency door open should be performed only when spinning is completely stopped Should not exceed the rated speed or specific gravity Samples whose density is greater than 1 2g ml must have reduced maximum rotational speed to avoid rotor failure The sample content should not exceed 80 of total capacity of a tube Otherwise it would cause spillage of sample fluid and even the tube breakage ALWAYS load the tubes symmetrically with evenly weighted samples to avoid rotor imbalance If necessary use the water blank to counterbalance the unpaired sample The operation speed should not exceed the highest value of the individual guaranteed g forces of each centrifuge rotor bucket or adaptors and sample container especially the guaranteed g force 7 arr LIZEN Low Speed Centrifuge 416 14 15 16 17 18 LY ROUEN Low Speed Centrifuge 416 of sample container should not be neglected The rotors should be cleaned and kept dry after every use for longer life and safety ALWAYS disconnect the power supply prior to maintenance care and service to avoid electrical shock ALWAYS use proven disinfection procedures after centrifuging biohazardous materials Should not centrifuge flammable toxic radioactive explosive or corrosive materials When it is necessary to use toxic or radioactive materials or pathogenic mic
9. e the Door Locking Nut clockwise until tightly assembled gt To disassemble the rotor Rotate the Door Locking Nut counterclockwise 3 Load the 15ml stainless steel sleeves at every hole v When you couple the rotor at first installation you turn off the instrument v After coupling the rotor turn on the instrument FR 13 Li LIZEN Low Speed Centrifuge 416 4 3 Positioning of Sample Tubes Action 1 Before loading sample tubes check the water drop or dirt in the rotor hole or inner adaptor gt If there is a water drop or dirt in the rotor hole or inner adaptor remove it with soft dry cloth 2 Tubes should be placed in the rotor with same amount of samples and at symmetrical positions gt Only use appropriate centrifugal tubes and do not exceed the speed beyond the tube s max g force gt For safety fill the sample for 70 80 in the tubes A Correct Way of Sample Balancing and Tube Usage 9 000 xg 8 000xg 10 000 xg TUR vr Check the g force of sample containers A e 10 80 filling for safety If the number of samples is not in pair please load the control tubes at each symmetrical position Otherwise it results noise and vibration which eventually damage the instrument Ke For safety the Imbalance Cut Off function will be occurred if there is imbalance of loading tubes Error 8 Imbalance error Please refer to 7 Trouble Shooting FR 14 Li LIZEN Low
10. eong dong Yuseong gu Daejeon 305 301 Korea declare under our sole responsibility that the product Description of Equipment Centrifuge Model Name 416 to which this declaration relates is in conformity with the following Standards or other normative oratory use Part 1 General Safety requirements for electrical r measurement control and laboratory use Part 2 Particular requiremen Electrical equipment for measurem EMC requirements Industrial scientific and medical ISM disturbance characteristics Limits a 2004 108 EC Directive the European Parliament and of Council of 15 December 2004 on the approximation of the laws of the Member States relating to electromagnetic compatibility and repealing Directive 89 336 EEC 2006 95 EC Directive 2006 95 EC of the European Parliament and of the Council of 12 December 2006 on the harmonization of the laws of Member States relating to electrical equipment designed for use within certain voltage limits Issued date November 11 2008 Confirmed Biba ez Sun Ki Kim President YR 28 3 LIZEN Low Speed Centrifuge 416
11. eping holding finger on the A V buttons for 5 A V seconds the unit of setting value is changed to 10 rcf from 1 rcf 3 Press the RPM RCF button again for saving C A 5 3 Setting Time gt Speed setting unit 1min or 10min 1 sec or 10 sec Action 5 3 1 Setting MIN g Pra 1 Press the TIME button once gt Minutes MODE is generated by pressing a TIME Button once 2 Press the A V buttons to change input value gt After keeping holding finger on the Aw buttons for 5 NN seconds the unit of setting value is changed to 10min from 1 5 VY min b J 3 Press the TIME button again for saving 3 16 Li LIZEN Low Speed Centrifuge 416 5 3 1 Setting SEC An 1 Press the TIME button twice gen gt Seconds MODE is generated by pressing a TIME button twice Le ZA TIME k J 2 Press the A V buttons to change input value gt After keeping holding finger on the A w buttons for 5 seconds FIT ln HI a SEC Save the unit of setting value is changed to 10 sec from 1 sec A 3 Press the TIME button again for saving 5 4 Start Stop Action 1 After setting RPM RCF and Time press START STOP button gt During running a Start LED is turned on edo Gei START RPM TIME EZA RCF A gt In case of pressing the START STOP button while running the running is stopped 5 5
12. essing A V button HUH LANI XZ O PROG 3 Press PROG button once again Xe gt The setting values are displayed according to your saved number gt If you do not press the PROG buttons for 5 second the setting mode is cleared b 18 Li LIZEN Low Speed Centrifuge 416 5 7 Emergency Door Open For emergency door open you can use the Emergency Door Open Tool as long as the instrument is completely stopped The door can be unlocked manually with Emergency Door Open Tool through the emergency opening hole 1 Find the emergency hole on the left side of the instrument 2 Insert the Emergency Door Open Tool into the hole and push it until the door is released Manual opening should be performed only when spinning is completely stopped Otherwise harmful damage will be accompanied to not only operators but samples After opening the door manually it is recommended to wait until normal electricity comes back 5 8 Fuse replacement When the power is not turned on please check the connection of power consent power switch Replace the fuse as following instruction if the power is still not turned on Action 1 Separate the AC Power Cord at the back of the instrument and push the flat head screwdriver for bring out the fuse case Connected Fuse in the power 2 Replace the damaged fuse with new one from the fuse case and then connect in the power 19 LY ROUEN Low Speed Cent
13. ew bolts are installed at the bottom of each instrument for keeping the motor on place As depicted in the following pictures the length of one bolt in the front bottom is longer than the instrument s feet for operator in order to recognize the wobbly status These bolts and nuts should be removed before the installation of the instrument 1 Open the box and lift out the instrument carefully 2 Place the instrument on the solid and flat table 3 To disassemble the Motor Protecting Devices use the additionally supplied 6mm wrench beta and unscrew and remove all 2 bolts It is now ready for installation re Place the instrument on the solid and flat table 4 Installation 4 1 Power On Off and Door Release Action 4 1 1 Power On Off 1 Connect the AC Power cord at the power socket on the right back of the instrument 2 Turn on the instrument by pressing a switch on the right side of the instrument 3 Press the Door button to open the door 11 La TYROZEN Low Speed Centrifuge 416 4 1 2 Door Release 1 For opening the door press the DOOR button gt The door is automatically opened after completion of spinning with beeping sound gt Close the door until hearing clank shut gt When the door is opened the door LED turns on v The door is not opened while the instrument is running v If the door is opened the instrument could not operate even with pressing the Start button v Po
14. he running rotor the matching status of Rotor Lid with the rotor Please refer to 4 2 Rotor Coupling and Disassembling Check balances of samples in the rotor Please refer to 4 3 Positioning of Sample Tubes and load the same weight of samples symmetrically rR 21 Li LIZEN Low Speed Centrifuge 416 7 2 Error Code If the instrument shows the error code with beeping sound press STOP button to stop the beeping sound and press Enter button to release of the error status and make the instrument go to the default setting again Error Error 1 or Error 9 Error 2 Error 3 Error 4 Error5 Possible Causes RPM Sensor Door Motor Overheating Low Voltage High Voltage Actions Shut off the power supply and then turn on the power switch again to check the instrument If the error code shows continuously although you try to operate again please call Gyrozen Field Service Engineer If the door is not closed completely this message is appeared Remove the dirt at the door latch and then close the door completely again Check the Door LED on the display window If it is not solved in shortly open the door with emergency door tool manually for safety of sample Please refer to 5 7 Emergency Door Open If the motor is overheated this message is appeared Shut off the power supply for an hour and then turn on the power switch for checking the instrument If the error code shows continu
15. mes reminder b 6 Li LIZEN Low Speed Centrifuge 416 1 2 Safety Precautions Before using the instrument please read this operation manual to ensure correct usage through understanding Incorrect handling of the instrument could possibly result in personal injury or physical damage on the instrument or its accessories 10 Lil 12 13 ALWAYS locate the instrument on a flat rigid and stable table capable of withstanding the weight of the instrument and its spinning operation ALWAYS make a safety zone of 30 cm around the centrifuge to indicate that neither hazardous materials nor persons should be permitted within the area during operation v ALWAYS position the instrument with enough space on each side of instrument to ensure proper air circulation ALWAYS install the instrument within a temperature and humidity controlled environment Permissible ambient temperature 5 C 35 C Relative humidity lt 85 Before connecting the power check the rated voltage Should not use unapproved rotors and accessories v Only use rotors from Gyrozen Co Ltd with appropriate centrifugal tubes and suitable adaptors to embrace sample containers tightly enough inside rotors Before operating the instrument check if the rotor and the rotor lid are securely fastened v Should operate the instrument with a rotor properly installed and secured to the motor shaft Mount the rotor on the motor shaft properly che
16. ously please call Gyrozen Field Service Engineer If the power input of Power supply V Hz is 10 less than required power this message is appeared Shut off the power supply and then check the voltage of the Power supply V Hz Use AVR to provide proper power If the power input of Power supply V Hz is 10 more than required power this message is appeared Shut off the power supply and then check the voltage of the Power supply V Hz Use AVR to provide proper power ba 22 Liv LIZEN Low Speed Centrifuge 416 If the instrument is soun with over speed there will be some problems in the overload of motor and the output of motor Error6 Over Speed l l Shut off the power supply and then turn on the power switch again to check the instrument If the installed software has bugs this message is appeared Error 7 Software l l Tuning the firmware Download Check weight balances of samples Please refer to 4 3 Positioning of Error 8 Imbalance l l Sample Tubes and then turn off and on the instrument for checking Any wire disconnection or tuning of the instrument must be performed only by a service engineer who is authorized by GYROZEN Co Ltd b 23 Li LIZEN Low Speed Centrifuge 416 8 Rotors and Accessories Swing Rotor GR5 5 100 4 4 loadings MOK r LI E UI Tube ij ABA Tube capacity mQ 1 5 2 0 2 6 7 4 10 9 15
17. rifuge 416 6 Maintenance 6 1 Outer part of instrument 1 Clean the outside of the instrument with dry soft cloth If necessary dip the cloth in neutral detergent and clean contaminated area Keep completely dry after cleaning 2 Do not use any volatile chemicals such as alcohol and benzene etc 3 Be careful not to make scratches on the surface of the instrument The scratches can cause corrosion on the surface of the instrument v I any rust appears clean it with neutral detergents and keep dry 6 2 Chamber 1 Keep dry inside the chamber after every use 2 If the chamber is contaminated dip the cloth in neutral detergent and clean contaminated area 6 3 Shaft 1 Always make special attention to clean the motor shaft to avoid any imbalance problem due to the contaminants 2 After using the instrument take out the rotor from the shaft and clean the shaft with dry soft cloth to keep dry 6 4 Rotor 1 If any parts are contaminated with samples clean the rotor with soft wet cloth and keep the rotor dry 2 Be careful not to make scratches inside or on the surface of rotors Any small scratches can cause corrosion of the rotor and big damage to the instrument 3 If you do not use the instrument keep the rotor separately from the motor shaft and stand it upside down 6 5 Transportation of the instrument 1 If you need to move or ship the instrument be cautious to protect the motor shaft from any
18. ro organisms which belong to the Risk Group II of WHO Laboratory Bio safety Manual should follow national regulations Do not place dangerous materials within 30 cm distance around the instrument and that is also recommended by IEC 61010 2 020 Use the emergency door open function only when the door button on the control panel is dumb under the condition of complete stop of rotor running Never try to open or move the instrument if it is not completely stopped If the power input is more than 10 of the recommended voltage or fluctuates frequently it may cause malfunction of the instrument and often result serious damage Install the instrument at the place without any kinds of corrosive gases 2 Product Description amp Technical Specifications 2 1 Product Description Door errira Chamber Power er socket Daui e gun E _ e ov a Emergency Door Br Open Hole g Si ede switch Control Panel Accessory EI e gt 7 e User manual AC Power Cord Rotor Locking Tool Emergency Open Tool Lubricant grease 9 Li LIZEN Low Speed Centrifuge 416 2 2 Technical Specifications Max capacity KS This instrument has following functions for safety 1 SOFT spin key for gentle acceleration and deceleration 2 Automatic detection and alarms for imbalance excess speed and heating b 10 Li LIZEN Low Speed Centrifuge 416 3 Unpacking Motor Protecting Devices 2 ea of scr
19. ty and long life m High quality cabinets with scratch resistant powder coated finish EASY CUSTOMIZATION Any rotors sample containers and adaptors can be manufactured according to customer s5 Specification Flexibility of including any additional functions or programs in need b 2 Li LIZEN Low Speed Centrifuge 416 CONVENIENCE IN OPERATION m Intuitive touch button with easy to follow controls a Easy to read LCD display m Automatic RPM RCF conversion for prompt detection of g force m Easy to check actual rpm through the top window of door Program memory for up to 10 programs m Automatic door opening function when rotor is static for easy retrieval of samples A large assortment of rotors fixed angle or swing out with buckets and adaptors PATENTED TECHNOLOGIES Unique efficient air cooling flow to reduce Spinning frictions so that the temperature increase and noise are minimized ECO FRIENDLY MANUFACTURING E Dust free AC induction motor Mi Eco safe refrigerant R404a E Very quiet operation at lesser than 56 3 LY ROUEN Low Speed Centrifuge 416 1 1 1 2 2 1 2 2 4 1 4 2 4 3 5 1 3 2 5 3 5 4 5 5 5 6 5 5 8 6 1 6 2 6 3 CONTENTS Page Meanings of symbols NNN RR 6 ed oe deie A iii 6 BN ERE ____ W WWW T 7 Product Description amp Technical Specifications a 9 Product Description a 9 Technical Specification a 10 MN a E i 11 Installation
20. wer Failure If there is any power failure during operation door is not opened with Door button Door can be opened only when the operation is completely stopped and the power is on again If you want to open the door at the power failure please refer to 5 7 Emergency Door Open 4 2 Rotor Coupling and Disassembling Action 1 Before coupling a rotor clean the motor shaft and chamber with soft dry towel 4 2 1 Swing Out Rotor 2 Mount a proper rotor into the motor shaft 3 Grasp the rotor with one hand and place Rotor Locking Tool at the center hole of the rotor gt To assemble the rotor Rotate the Rotor Locking Tool clockwise until tightly assembled gt To disassemble the rotor Rotate the Rotor Locking Tool counterclockwise 4 Hang the appropriate buckets into the rotor gt Load the identical bucket at each wing for safety Do not leave a vacant wing without bucket All wings should hold identical bucket FR 12 Li LIZEN Low Speed Centrifuge 416 gt Remove dirt and dust around hooks of rotor and hanging part of bucket 5 Spin the rotor manually to check if bucket swinging is free enough and ever If they do not swing freely apply the Lubricant grease to the link area 4 2 1 Fixed Angle Rotor 2 Mount a proper rotor into the motor shaft Put the Washa 7 at the center hole of the rotor and assemble it with the Door Locking Nut P j gt To assemble the rotor Rotat
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