Fastpette FP/02/05 OK - Labnet International
Contents
1. FASTPETTE 4 um Par eo az aes E Labnet International Inc Woodbridge NJ 07095 USA PO Box 841 Phone 732 417 0700 Fax 732 417 1750 email labnet labnetlink com www labnetlink com FASTPETTE Aspiration button ABS Membrane filter PP PTFE Dispense button ABS J Safety valve Suction speed switch PP Connector gasket SI Dispense mode switch PP Low battery indicator Nosepiece PP NiMH battery Pipette holder SI Casing PP FASTPETTE 1 1NTROBUGTION Cosina iii 2 2 WORK SAFETY INSTRUCTIONS na ann 2 3 LIMITATIONS OF USE uan BE ee ea 2 4A GETING STARTED nee eee 3 5 ASPIRATING AND DISPENSING LIQUIDS 3 G TROUBLESHOOTING 4 7 REPLACING THE FILTER AND CLEANING THE VALVES 5 8 CHARGING THEBATIERY u seen 6 9 MAINTENANCE rise ie ian ire aia eno ea 6 102 COMPONENTS 2 laa 7 11 ORDERING 7 12 SPARE PARTS a E2. charging When the battery is completely charged the charging circuit disconnects automatically When charging is complete the control lamp fig 1E will shine continuously Charging 1 Ambient charging temperature 10 to 30 C 2 Charging is accomplished with the charger included with the FASTPETTE During charging the lamp indicator will flash 3 A full charge is accomplished in 11 14 hours 4 When charging is complete the indicator lamp will glow steadily The pipette controller has a NiMH battery with a capacity of 1700 mAh The service life of the battery is approximately 1000 charging cycles For optimum battery life the battery should be completely discharged before recharging The time circuit of the charger prevents overcharging of the battery Note Allow the battery to charge fully and do not interrupt the charging cycle If the cycle is interrupted the battery should not be recharged until it is completely discharged 9 MAINTENANCE The FASTPETTE requires no routine maintenance The external parts of the pipette controller may be cleaned by wiping down with isopropyl alcohol The nosepiece and pipette holder with check valve may be autoclaved at 121 C for 20 min utes The filters included in the set may be sterilized by autoclaving at 121 C for no more than 15 minutes Attention After autoclaving allow the silicone pipette holder to cool and then bend and squeeze it several times in the area of
3. A rien 7 4 1 INTRODUCTION The FASTPETTE pipette controller is designed for pipetting liquids with serological pipettes The device works with all types of glass and plastic serological pipettes in the range of 1 ml to 100 ml The FASTPETTE is equipped with replaceable membrane filters that protect against cross contamination The filters also prevent contamination of the mechanism by reagent vapors The FASTPETTE features a two speed control system which enables very fast dispensing of large volumes and precise measuring of small volumes Fig 1 shows the external parts of the pipette controller and a description of the materials used in the construction 2 WORK SAFETY INSTRUCTIONS Before operating the FASTPETTE all users should read these instructions carefully Using the pipette controller a manner inconsistent with the operating instructions may result in damage to the device Any required repairs to the FASTPETTE should be performed at an authorized service center Repair elsewhere may void the warranty e While using the FASTPETTE follow general laboratory safety guidelines Protective clothing goggles and gloves should be worn FASTPETTE shall be used for measuring liquids only in the manner specified in this manual Liquids to be measured are limited by the chemical and mechanical resistance of the device as well as user safety pipette controller should not be used in environments whe
4. The filter is dirty fig 1H the pipette out of the holder the pipetting aid does not Disassemble the pipette holder draw liquid or draws liquid Take out the filter check it for very slowly impurities if it is dirty replace it with a new one FASTPETTE The check valve is blocked Check the valve for flooding fig 1J If it is wet rinse it and dry Check the valve for blockage or displacement due to excessive force during pipette installation if it is blocked or displaced try to set it in the right position The pipette holder Check the pipette holder and the and or the connector gas connector gasket for mechanical ket is damaged fig 1K damage if there is damage replace the damaged part with a new one Liquid leaks from the The pipette is damaged Check the pipette for damage pipette the aspiration cracks dents if present repla and the dispense buttons ce the pipette with a new one are not pressed The pipette is inserted Check whether the pipette has incorrectly been correctly inserted in the holder The pipette holder the filter Check whether all parts are pre or the connector gasket sent and correctly installed is installed incorrectly The pipette holder and or Check the pipette holder and the the connector gasket is connector gasket for mechanical damaged fig 1G fig 1K damage if there is damage replace the damaged part with a new one If th
5. ator is lit the pipette controller must be charged before use Alternatively the charger can be connected and the FASTPETTE can be used while charging Only the original charger should be used The line voltage must conform to the specification on the charger Follow the instructions in section 8 of this manual for charging the FASTPETTE 5 ASPIRATING AND DISPENSING LIQUIDS Attaching a pipette Before attaching a pipette check for damage The pipette should be free of dents chips and sharp edges on the section that is held by the pipette controller Be sure that this section is dry Hold the pipette close to the end that is to be inserted in the pipette controller and push it into the pipette holder until resistance is felt fig 2A Do not apply excessive force as this may cause the pipette to break and injure the user A pipette that is correctly seated in the holder will not tilt to the side After seating the pipette hold the pipette controller so that the pipette is in a vertical position pointing downward lt is possible to lay the pipette controller on the bench stand with a pipette attached It is not recommended to leave a pipette attached to the device for an extended period of time ie overnight Do not lay the pipette controller on its side while there is liquid in the pipette Filling the pipette Before aspiration is started set the suction speed using the HIGH LOW switch fig 1C HIGH position fast aspiratio
6. e problem cannot be remedied using the above guide contact Labnet International to obtain service Before returning the FASTPETTE for service it should be cleaned and decontaminated A decontamination form and RA number must be obtained from Labnet before sending for service 7 REPLACING THE FILTER AND CLEANING THE VALVES Follow the safety instructions in section 2 when disassembling the device If over time the drawing efficiency of the pipette controller deteriorates the cause may be a dirty filter If this is the case the filter must be replaced following the directions below and illustrated in figure 3 Remove the pipette Unscrew the nosepiece fig Remove the membrane filter fig and the pipette holder fig Rinse the holder with the safety valve using a wash bottle fig Blow any remaining liquid out of the holder and allow it to dry completely before reassembling Install new membrane filter fig 3D and assemble the device in reverse order 8 CHARGING THE BATTERY The FASTPETTE has a low battery indicator When the battery requires charging the indicator will light when the aspiration button is depressed Note The FASTPETTE should be charged only with the original charger The line voltage must conform to the specification on the charger Use of a charger other than that supplied may damage the battery of the pipette controller A time circuit controls battery
7. itch fig 1D GRAV position When the GRAV mode is selected the liquid in the pipette is dispensed by gravity flow only Flow rate is adjusted by the pressure applied to the dispense button Attention During gravimetrical dispensing the pipette is not completely emptied due to the characteristics of pipettes used with the pipetting controller BLOW position the liquid in the pipette is dispensed in constrained mode the liquid is forced out of the pipette The dispensing speed is regulated by the depth of pressing applied to the dispense button When the button is pressed to the end position the rapid emptying with the blow out is executed Attention Due to the characteristics of graduated pipettes in order to diminish the splashing of the liquid in the last stage of emptying the pipette the speed of the dispensing should be reduced 6 TROUBLESHOOTING If the pipette controller fails to function properly refer to the troubleshooting table below to isolate and remedy the problem Problem Possible cause Action The pipette falls out the The pipette holder is dirty Take the pipette out of the holder holding force of the pipette or wet fig 1G Disassemble the pipette holder is to small or tilts to the and check for mechanical side too much damage Clean wash and dry the pipette holder The pipette holder is dam If the holder is damaged replace aged it with a new one The pump is working but
8. n LOW position slow aspiration It is recommended to choose the LOW position for pipettes with a volume of up to 5 ml and the HIGH position for pipettes with a volume greater than 5 ml Holding the pipette con troller so that the pipette is in a vertical position immerse the pipette end in the liquid to be drawn up fig 2B and pres the aspiration button gently The filling speed depends on how much pressure is applied to the aspirating button The more pressure applied the faster the liquid is drawn up It is recommended to draw a slightly greater volume of liquid than is required Adjust the aspiration speed while filling as to not overfill the pipette Setting the volume After filling the pipette wipe the tip with absorbent paper to remove any liquid from the outside surface of the pipette The volume can then be precisely adjusted Gently press the dispensing button fig 2C until the meniscus of the liquid in the pipette aligns exactly with the required volume mark The speed of aspiration can be adjusted by varying the pressure applied Emptying the Tilt the receiving vessel and place the pipette tip in contact with the vessel wall Gently press the dispense button fig 2C The speed of dispensing is adjusted by varying the pressure on the dispensing button The greater the pressure applied the faster the liquid flow The FASTPETTE has two dispensing modes The modes are selected with the GRAV BLOW sw
9. re there is a risk of explosion Information and instructions provided by the reagent manufacturer must be observed Use only original spare parts and accessories Only the original charger supplied by the manufacturer should be used for charging the battery and powering the device e If the pipette controller malfunctions discontinue use immediately Clean the device in accordance with the operating instructions and contact the manufacturer for repair Avoid excessive force while using the pipette controller 3 LIMITATIONS OF USE Substances that damage PP SI ABS EPDM and POM should be measured with the FASTPETTE pipette controller shall not be used in environments where a risk of explosion is present Flammable liquids shall not be measured in particular substances with a flash point below 0 C ie ether acetone pipette controller shall not be used for drawing acids with concentration above 1 mol pipette controller shall not be used with substances with a temperature above 50 C ambient operating range of the pipette controller is 10 to 35 C FASTPETTE Before operation be sure that the pipette controller battery is charged To check the battery charge press the aspiration button fig 1A and check the low battery indicator light If the indicator does not light the pipette controller is ready for use If the low battery indic
10. the safety valve Storage Store the FASTPETTE in a dry place The storage temperature should be between 20 C and 50 C When not in use the pipette controller should be placed in the bench stand included with the unit or stored using the optional wall hanger Note Do not store the FASTPETTE with a pipette attached FASTPETTE The FASTPETTE pipette controller is supplied with the following components Charger Bench stand e Membrane filter 0 2 um 2 pieces e Instruction manual Then bench stand has 2 positions for storing the FASTPETTE fig 4 11 ORDERING INFORMATION The following versions differ with the types of included charger The appropriate version should be ordered depending upon the line voltage of the facility Versions Voltage Catalog number USA 120 60 Hz P2000 EURO 220 V 50 Hz P2000 220EU UK Ireland 230 V 50 Hz P2000 230UK Australia 240 V 50 Hz P2000 240AU 12 SPARE PARTS in fig 1 Description Catalog no F Nosepiece P2022 G Pipette holder P2021 H Membrane filter 0 2 um P2023 Membrane filter 0 45 um P2024 K Connector gasket P2025 L USA 120V 60 Hz P2017 Power EURO 220V 50Hz P2015 supply UK Ireland 230 V 50 Hz P2016 Australia 240 V 50 Hz P2018 M Bench stand P2029 N Wall hanger P2028 FP 02 2005 N FASTPETTE
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