4861722 User Manual SCO26H
Contents
1. 50 OR 50 e o w M 50 CD EA A AA A AEAT E A EEE 51 WTO UE a E EMI 51 d 51 Pressure Conversion CA iranica 51 Rd ne ee de E de leon eee ou Mat cc Le 52 PARTS EST Lili 55 Ordering Parts and Consumables iii 55 4 Page LAB INTRODUCTION Thank you for purchasing a Sheldon Manufacturing Inc SCO26H Humidified CO Incubator We know that in today s competitive marketplace customers have many choices when it comes to constant temperature equipment We appreciate you choosing ours Our continued reputation as a leading laboratory product manufacturer rests with you We stand behind our products and will be here for you if you need us These incubators are intended for laboratory industrial and educational microbiological cultivation applications These incubators are not intended for use in hazardous or household locations Before you use the SCO26H read this manual in its entirety to understand how to install operate and maintain the incubator in a safe manner Keep this manual available for use by all operators Ensure that all operators are given appropriate training before the incubator begins service GENERAL SAFETY CONSIDERATIONS Note Failure to follow the guidelines and instructions in this ma2. 18 CONTROL PANEL OVERVIEW dud 20 OPERATION 22 EOV OF ODS VAT ON mM 22 Preparing the INCUDRAIOFIOMUSE silla 25 Set the Temperature Set POINL iii 27 Muting the Audible Temperature Alarm inserer 28 Au Om AC DOON TOI PERO RATE ERNIA I TOR 28 QUOS SPORE STRO SO 29 Muting the Audible COS Alar ie 30 NO CAS SUPOVA OO eisrean riali ar 30 SC AO NEO MCN E AA E E E EE E E AEE 31 Temperature Accuracy Verification sise 32 CACC ICY VENI zione 34 Set the Over Temperature LImMit iii 36 LOG AE COMORES men ee r do die nt oo 37 Pags a c EB E 37 PC CES SOY CANO HH 38 DIARI na 38 aS Iz pem E 39 Cleaning and Disinfecting ii sss rss sa asas sss saa aas s nasa aa sons 39 MINIMIZING Contamination Exposure ss 40 HEPA Filter and GAS LINCS iieiea 41 3 Page LAB Storage of th SCOZGH IncubalOF oit tr ern ok aden s dues AENEA VN FR Rod ce mens RETREAT CR REEE 41 Maintaining Atmospheric Integrity seen 41 FIGCHICAl COMPONONIS ica EE 41 Calibrate the Temperature display is 42 Calibrate H COS DISDla nce 46 UNE SPECIEICATIONS cS 50 VE
3. SET THE HUMIDITY LEVEL The SCO26H humidification system is intended to provide relative humidity to slow or prevent sample media from drying out in the chamber From its minimum to maximum setting the Set Humidity Control dial provides roughly 50 90 relative humidity in the chamber The exact RH is dependent on the chamber air temperature the ambient humidity of the laboratory space the incubator is located in and on the volume of sample media in open or breathable containers inside the chamber 1 When carrying out the Preparing the Incubator procedure set the Set Humidity dial to its midpoint a The incubator must operate humidified if you will be performing a temperature display accuracy verification or calibration 2 Once samples have been loaded allow the incubator to operate continually for 2 3 days a Monitor sample media levels 3 After 2 or 3 days adjust the Set Humidity control dial a Increase the humidity level by turning the dial to the right clockwise if samples are drying out too quickly b Leave the dial at the midpoint position if sample media is drying at an acceptable rate the sample media is not drying out and condensation in the chamber is c Decrease the humidity level by turning the dial to the left counterclockwise if 4 excessive The dial setting will need to be adjusted if there are significant changes in the volume of sample media in the chamber The humidification sys
4. LIFTING AND HANDLING The unit should only be lifted by its bottom surfaces using proper heavy lifting machinery such as a forklift or pallet jack Handles and knobs are inadequate for lifting or stabilization The unit should be completely restrained from tipping during lifting Transporting the unit while lifted is not recommended and may be hazardous Remove all moving parts such as shelves and trays and secure the door in the closed position prior to lifting the unit Do not attempt to move the unit while in operation or before the unit has cooled LEVELING The unit must be level and stable for safe operation Each unit ships with four leveling feet 1 Insert one leveling foot into each of the four holes in the bottom corners of the unit AN 2 Adjust the foot at each corner until the unit stands level and solid without rocking 2700506 To raise a foot turn it in a counterclockwise direction Figure 6 3 To lower a foot turn it in a clockwise direction Leveling Foot To prevent damage to the feet while in transport turn all feet to the maximum counterclockwise position INSTALL INCUBATOR IN LOCATION Install the unit in a workspace location that meets the criteria discussed in the previous entries of the Installation section 13 Page LAB INSTALLATION CONTINUED Connection CONNECT TO THE WATER SOURCE Relief Valve Cap Optional The incubator water source can be a pressured distilled or potable wa
5. Symbol Definition Indicates Potential Shock Hazard Signale danger lectrique Indicates the unit should be recycled Not disposed of in land fill Indique l appareil doit tre recycl Ne pas jeter dans une d charge Indicates CO gas Indique gaz CO2 Indicates relative humidity RH Indique humidit relative RH 2 5 IX gt 19 Page LAB CONTROL PANEL OVERVIEW Set Temperature C Set CO2 Set Humidity Level High High m i Mute bl Mute Low Low ex 5 o A o e o HUMIDIFIED CO INCUBA TOR SERIES Sheldon Manufacturing Inc J Figure 13 Control Panel Over Temperature Limit Thermostat OTL This graduated dial sets the heating cut off point for the OTL temperature limit system The OTL system prevents unchecked heating of the chamber in the event of a failure of the main digital controller For more details please see the Over Temperature Limit System description in the Theory of Operations page 24 The red Over Temp Activated light illuminates when the Over Temperature Limit system cuts off heating by rerouting power away from the heating elements Power Switch The power switch controls all power to the incubator and its systems Power is supplied when the switch is in the 1 on position and the Power On light illuminated Temperature Control and Display Labeled Set Temperature C this display shows the current air temperature in the incubation
6. 36 Page LAB OPERATION CONTINUED LOAD THE CHAMBER Sheldon Manufacturing recommends allowing the incubator to operate for at least 8 hours at your application temperature CO and humidity set points after a cold start before introducing samples This to allow the chamber to achieve the set points and stabilize Place sample items and any accessories on the shelves inside the chamber as evenly spaced as possible Good spacing allows maximum air circulation a higher degree of temperature uniformity and reduced condensation CONDENSATE DRAINAGE Some condensate will be present on the incubation chamber walls when the unit is humidified The amount is dependent on the chamber RH level the ambient RH the chamber temperature and the number of open media containers in the chamber Condensate appears whenever the chamber relative humidity matches or exceeds the dew point The dew point is the humidity level at which the air cannot carry additional water vapor The higher the chamber air temperature the higher the dew point will be as warmer air carries more water vapor than cold air The chamber form factor is designed to channel condensate to a drain set in the chamber floor The drain exits the back of the unit and has a water filled vapor trap a U shaped dip which chamber atmosphere air from leaking If the incubator has been out of service for a prolonged period of time refill the vapor trap with a half cup of water 240m
7. C for incubation applications The CO range is a 0 20 concentration The incubator features a heated glass viewing door that allows visual inspection of samples without compromising the chamber CO or humidity environment Heating When powered the incubator heats to and maintains the incubation chamber air temperature at the currently programed temperature set point An internal microprocessor stores the temperature set point The microprocessor board is wired to a solid state temperature probe located in the chamber airstream on the rear wall When the processor detects that the chamber temperature has dropped below the temperature set point it pulses power to a heating element located in a recirculation air duct above the chamber Once the chamber temperature is stable most heating pulses correct for deviations of less than 0 1 C The processor employs proportional integral derivative analytical feedback loop functions when measuring and controlling the chamber air temperature PID controlled heating pulse intensities and lengths are proportional to the difference between the measured chamber temperature and the current set point The frequency of pulses are derived from the rate of change in that difference The integral function slows the rate of pulses when the temperature nears the set point to avoid overshooting The SCO26H relies on natural heat radiation for cooling The incubator can achieve a low end temperature of the ambi
8. The kit is purchased separately from the incubator Never connect the kit to a deionized water source The kit will not prevent deionized water from damaging your unit 18 megohm cm of resistivity can be achieved with the SWPK The Adsorber filter removes free chlorine organics phosphate complexes and turbidity The Universal filter removes ionizable constituents except free carbon dioxide and silica The Universal filter reduces the ion concentration level equivalent to a single distillation Filter Replacement The black Adsorber filter is an active charcoal filter It typically will last for 6 months to 1 year of use depending on the water quality of your laboratory or workspace tap water source and the humidity setting of the incubator The mix colored Universal filter will gradually turn yellow starting at the top and moving to the bottom while in use The filter s lifespan is highly dependent on local water quality It should be replaced when the mixed color filter media has turned predominantly yellow around the entire circumference down to approximately 2 5 inches 6 cm above where the bottom white cap meets the body of the cylinder Universal Fliter Valve Water Source Regulator Drain Pan Adsorber Figure 5 Purification Kit Plumbing Part Number SWPK Please see the Ordering Parts and Consumables entry on page 55 for ordering the SWPK 12 Page LAB INSTALLATION CONTINUED
9. The volume of sample media inside the chamber stored in open or breathable containers The following graphs show the range of achievable incubation chamber RH and temperature levels under varying ambient conditions Humidity and Temperature Performance Ambient Conditions 20 C 50 RH 90 T Chamber 45 Condensation 42 Normal Temperature and Chamber Humidity Operations M Ca de Temperature Temperature Instability C s j 39 52 Page 50 55 60 65 70 75 80 85 90 95 Chamber RH UNIT SPECIFICATIONS CONTINUED Room Conditions 50 RH Humidity and Temperature Performance Ambient Conditions 25 C 50 RH 50 C T Chamber 45 Condensation jo mmi 42 Minor Chamber Temperature Chamber Instability Temperature C i 3 Major Chamber Temperature Instability 35 VAN Smo 50 55 60 65 70 75 80 85 90 95 Chamber RH 30 D _ A 53 Page LAB UNIT SPECIFICATIONS CONTINUED Room Conditions 50 RH 30 C Humidity and Temperature Performance Ambient Conditions 30 C 50 RH T Chamber Condensation 90 45 Normal Temperature and Chamber Humidity Operations Major Chamber Temperature a S Instability 3 39 50 55 60 65 70 75 80 85 90 95 Chamber RH 54 Page LAB PARTS LIST Description Parts Number Description Parts Number Power Cord 125 volt 9ft 5 in 2 86m NEMA 5 15P 7790
10. alarms will activate if the incubator CO level deviates 1 above or below the CO set point An audible alarm will sound immediately for a high deviation The low deviation audible alarm will sound after the visual low indicator alarm has been continually illuminated for 15 minutes This delay prevents the alarm from sounding whenever a door opening creates a short lived drop in gas concentration 1 To mute an alarm press and hold the CO Up or the Down arrow button until the amber Mute LED illuminates Q 2 The alarm will stay muted for the duration of the current temperature deviation 3 Another deviation of 1 will reactivate the audible alarm Deviation 15 20 CO Start Minutes Minutes Set Point 4 1 or lower Low Visual Alarm Audible No Gas Figure 15 Indicator I Alarm Alarm n95 High Gas illuminates Activates Activates Alarm Muted Figure 16 Gas Alarm Timeline No GAS SUPPLY ALARM NGS If the Low Gas deviation indicator is active for longer than 20 minutes a second alarm will activate The letters ngS will appear in the CO display to indicate No Gas Supply The alarm will remain active even if the incubator is turned off and turned back on The NGS Alarm will remain on until CO2 is restored to the chamber It may take several minutes of CO inflow to establish a concentration percentage 96 high enough to deactivate the alarm Figure 17 No Gas Supply 30 Page LAB OPERATION CONTINUED
11. be installed in the chamber to obtain the best temperature uniformity and prevent condensation from forming inside the port A second stopper may be installed on the outside of the unit to prevent dust from building up in the port but is not required Figure 11 Port Stopper in Access Port 1750504 Figure 12 Port Stopper 17 Page LAB GRAPHIC SYMBOLS The incubator is provided with multiple graphic symbols on its exterior and internal surfaces These symbols identify hazards and the functions of the adjustable components as well as important notes in the user manual Definition Indicates that you should consult your service manual for further instructions Indique que l op rateur doit consulter le manuel d utilisation pour y trouver les instructions compl mentaires Indicates Caution hot surface Indique Avertissement symbole de surface chaude Indicates temperature Rep re temp rature Indicates the Over Temperature Limit system Indique le syst me de d passement de temperature Indicates AC Power Rep re le courant alternatif Indicates I ON and O OFF rep re de la position MARCHE de l interrupteur d alimentation O rep re de la position ARRET de l interrupteur d alimentation Indicates protective earth ground Rep re terre lectrique Indicates Up and Down respectively Touches de d placements respectifs vers le HAUT et le BA 20 P PE d LAB GRAPHIC SYMBOLS CONTINUED
12. be out of use for more than 24 hours to prevent microbiological contamination such as fungus or mold Disinfect the unit before drying if the chamber and shelving have been exposed to pathogenic microorganisms or if required by your laboratory protocol 1 Depower the incubator 2 Useasoft cloth to dry the chamber surfaces MAINTAINING ATMOSPHERIC INTEGRITY Periodically inspect the door latch trim catch and gasket for signs of deterioration Failure to maintain the integrity of the door system shortens the life span of the incubator Replacement of the chamber and door gaskets are service level procedures that should be performed by a qualified service technician ELECTRICAL COMPONENTS Electrical components do not require maintenance If the incubator fails to operate as specified please contact your distributor or Sheldon Technical Support for assistance please see page 6 41 Page LAB USER MAINTENANCE CONTINUED CALIBRATE THE TEMPERATURE DISPLAY Note Performing a temperature display calibration requires a temperature reference device Please see the Reference Sensor Devices entry on page 9 for device requirements Temperature calibrations are performed to match the temperature display to the actual air temperature inside the incubation chamber The actual air temperature is supplied by a calibrated reference sensor device Calibrations compensate for drifts in the unit microprocessor controller as well as th
13. eum o acceptable range of your protocol repeat steps 3 7 up to two more times Set CO a Three calibration attempts may be required to successfully calibrate units that are more than 2 out of calibration If the CO readings of the display and the reference device still fall outside your protocol after three calibration attempts contact your distributor or Sheldon Technical Support for assistance b Three calibration attempts may be required to successfully calibrate units that are more than 2 out of calibration End of procedure LAB 49 Page UNIT SPECIFICATIONS The SCO26H Humidified Incubator is a 115 voltage unit Please refer to the incubator data plate for individual electrical specifications Technical data specified applies to units with standard equipment at an ambient temperature of 25 and a voltage fluctuation of 10 The temperatures specified are determined in accordance to factory standard following DIN 12880 respecting the recommended wall clearances of 10 of the height width and depth of the inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times WEIGHT 620lbs 282 kg DIMENSIO By inches Exterior W x D x H Interior W x D x H 39 3 x 37 0 x 78 5 inches 30 7 x 26 0 x 56 5 inches By centimeters Exterior W x D x H Interior W x D x H 99 7 x 94 0 x 199 4 c
14. helps prevent overshoots When the chamber concentration is stable CO injections take place in small bursts to correct for deviations less than 0 1 The incubator is not provided with a means to actively remove CO from the chamber atmosphere Air Circulation The incubator continually circulates air internally while powered An air stream of heated humidified and COz encriched atmosphere is forced through vent holes on back wall of the chamber and blows across the shelf space It is then pulled upward at the front of the chamber by the action of the blower fan and back into the recirculation duct The heating element is located in the recirculation duct Water vapor and CO are injected downstream of the heating element in the duct A Peltier effect thermoelectric cooler unit provides condensation management with a cold plate in the back wall air duct of the chamber as well as helping to maintain temperature uniformity in the wall duct and chamber An automatic cutoff switch located on the door frame disables air circulation and heating as well as CO and water vapor injections whenever the chamber door is open Humidity Control The incubator is provided with an internal water vapor generator for humidification of the chamber space The humidification system is operated by the same microprocessor board that controls the chamber temperature and CO The RH microprocessor uses a solid state thin film capacitive humidity sensor located on
15. psi to the throughout this procedure The CO display will read LO until enough CO has built up sufficient for the sensor to register a concentration greater than 096 Note The example below represents adjusting the CO set point from 3 to 5 1 Press either the Up or Down arrow button on the CO panel a he display will flash the letters SP for set point b Aflashing adjustable CO set point will appear in the display Note The display will automatically exit the adjustment mode after 5 seconds of inactivity with the last shown set point value saved 2 Use the Up or the Down arrow keys to adjust the set point to your application CO concentration New Set Point 3 Wait 5 seconds after entering the set point a he display will stop flashing and the set point is now saved in the controller processor The chamber will now automatically inject CO2 or allow the lie current level to decay in order to achieve your set point The display will revert to showing the current chamber CO Injecting to achieve concentration the new set point Note The CO display and injections can be set to off when in the set point adjustment mode Hold the down arrow after the blinking set point appears until the display reads OFF The incubator will cease injecting CO End of procedure LAB 29 Page OPERATION CONTINUED MUTING THE AUDIBLE CO ALARM Visual high or low deviation indicator
16. the chamber rear wall to monitor the relative humidity level of chamber atmosphere The vapor generator maintains a steady supply of mild temperature water vapor in a pressurized reservoir A refill float switch in the reservoir opens the water inlet solenoid on the back of the chamber when it falls below approximately 1 quart 880mm The generator injects vapor into the recirculation duct stream when the RH sensor probe indicates the test space humidity has fallen below the user selected level Humidification is controlled by PID algorithms similar to those used for controlling temperature and CO The lengths of humidity injections are proportional to the difference between the measured chamber RH level and the set level The frequency of injections are derived from the rate of change in the difference and slowed by the integral function when the chamber level nears the set level to prevent an overshoot Note Steam and heated water vapor Steam is at or hotter than the local boiling point Heated water vapor is cooler than the local boiling point The use of heated water vapor rather than steam for humidification allows a greater range of temperatures in the chamber It also enables improved temperature stability and uniformity by reducing the temperature difference between newly humidified air and air already in the chamber space LAB 23 Page OPERATION CONTINUED Physical and Data Access An access port on the left side of the
17. undisturbed for 8 hours supplied to achieve temperature and RH stability for example overnight A continual CO supply stream may be introduced a minimum of 2 hours with the incubator otherwise undisturbed prior to performing the calibration 46 Page LAB USER MAINTENANCE CONTINUED Calibrate the CO Display 1 Once the incubation chamber has stabilized with no fluctuations of 0 1 or Reference Device greater compare the gas reference device and chamber CO display mo readings C 70 e a Ifthe readings are the same or the difference between the two 2 falls within the acceptable range of your protocol the Set CO display is accurately showing the chamber CO concentration The CO calibration procedure is now complete If there is a difference between the two readings that falls outside the acceptable range of your protocol see the next step Reference Device 2 Adisplay calibration adjustment must be entered to match the incubator CO display to the reference device Set CO 3 Place the display in its CO calibration mode a Press and hold both the UP and DOWN Set CO arrow buttons simultaneously for approximately 5 seconds Release the buttons when the display shows the letters CO The display will begin flashing the current CO display value Note lf an arrow key is not pressed for five seconds the display will cease flashing and store the last displayed value as the new current ch
18. unit allows sensors such as thermocouples and humidity meter solid state probes to be inserted and left in the chamber without compromising the CO2 atmosphere An atmosphere sample port for independently verifying the CO concentration in the chamber is provided on the left side of the unit adjacent to an RS232 data communications port The com port outputs CO humidity and temperature levels once per minute as a digital log line Jack ports on the back of the unit provide the same outputs as analog 4 20 milliamp signals for use by a building monitoring system Please see the Data Outputs entry on page 38 for more details The Over Temperature Limit System OTL When set the OTL system prevents runaway heating in the event of a failure of the temperature microprocessor or its sensor probe by rerouting power away from the heating element whenever the temperature in the incubator chamber exceeds the OTL setting The OTL is provided with an independent hydrostatic temperature probe on the chamber back wall The system is set by the end user at approximately 1 C above the current chamber temperature typically when operating at the application temperature Because of its nature as a mechanical cutoff and its lack of PID analytics the OTL cannot deliver the same degree of temperature stability and measurement precision as the digital display and controls The OTL System should only be used as a means of heating regulation for the incubation cham
19. 2 falls within the acceptable range of your protocol the display is accurately showing the chamber air temperature The Temperature Verification procedure is now complete See step 2 if a difference falls outside the acceptable range of your protocol Reference Device If there is a difference between the two readings and the difference falls outside the acceptable range of your protocol perform a temperature calibration to match the Set Temperature display to the actual chamber temperature Set Temperature C a Please see page 42 in the User Maintenance section i EE x End of procedure LAB 33 Page OPERATION CONTINUED CO ACCURACY VERIFICATION Note Performing a CO display accuracy verification requires a gas reference device Please see the Reference Sensor Devices entry on page 9 for the device requirements Optional The SCO26H CO display was calibrated at the factory at a 5 concentration A display accuracy verification may be performed when preparing the incubator for use if required by your laboratory or production protocol If an error between the actual and displayed CO concentrations is discovered perform a temperature calibration Please see the Calibrate CO Display procedure on page 46 in the User Maintenance section Temperature A CO display verification may be performed simultaneously with the temperature display verification as long as the chamber door is not opened dur
20. 504 1800510 Access Port Stopper Size 7 CO Gas Tubing Kit with In bom Shelf Clips Requires 4 per ray de Line HEPA Filter Y Shelf 1250512 Fuse 5X20mm T16A 250V ss Stainless Steel Shelf 3300513 995 00019 Leveling Feet ORDERING PARTS AND CONSUMABLES If you have the Part Number for an item you may order it directly from Sheldon Manufacturing by calling 1 800 322 4897 extension 3 If you are not certain that you have the correct Part Number or if you need that specific item please contact Sheldon Technical Support for help at 1 800 322 4897 extension 4 or 503 640 3000 Please have the model number and serial number of the incubator ready as Tech Support will need this information to match your unit with its correct part LAB 55 Page ACCESSORIES The following accessories are available for the SCO26H CO Cylinder Regulator Dual Stage For use with a gas supply cylinder tank Part Number 7150509 Gas Line HEPA Filters In line HEPA filters should be replaced either once per year or when noticeable discoloration is present The filter is directional and must be installed facing in the correct direction The word IN is stamped on the rim of the filter assembly on the side that faces toward the gas source The filter is plumbed between two sections 2800525 of gas line Part Number 2800525 Shel Lab Water Purification Kit Removes minerals ions and turbidity from potable water P
21. ER MAINTENANCE Warning Prior to any maintenance or cleaning of this unit disconnect the power cord from the power supply Avertissement Avant d effectuer toute maintenance ou entretien de cet appareil d brancher le cordon secteur de la source d alimentation CLEANING AND DISINFECTING If a hazardous material or substance has spilled in the incubator immediately initiate your site s Hazardous Material Spill Containment protocol Contact your local Site Safety Officer and follow instructions per the site policy and procedures The incubator chamber should be cleaned and disinfected prior to first use Periodic cleaning and disinfection are required to prevent microbiological contamination Do not use spray on cleaners or disinfectants These can leak through openings and coat electrical components Do not use cleaners or disinfectants that contain solvents capable of harming paint coatings or stainless steel surfaces Do not use chlorine based bleaches or abrasives these will damage the chamber liner Warning Never clean the unit with alcohol or flammable cleaners Avertissement Ne jamais nettoyer l appareil l alcool ou avec des nettoyants inflammables Cleaning 1 Remove all non attached incubation chamber components and accessories shelves racks and any additional items if present 2 Clean the chamber interior with a mild soap and water solution including all corners 3 Take special care when cleaning cha
22. HUMIDFIED CO INCUBATOR 115 Voltage 2 ae I Installation and Operation Manual SCO26H Previously Designated 2428H SCO26H HUMIDIFIED CO INCUBATOR Installation and Operation Manual Part Number Manual 4861722 Revision May 15 2015 These units are compliant with CAN CSA C22 2 No 61010 1 2012 CAN CSA C22 2 No 61010 2 010 R 2009 UL 61010A 2 010 2002 UL 61010 1 2012 EN 61010 1 2010 EN 61010 2 010 2003 LAB TABLE OF CONTENTS INTRODUC TION reece lai 5 General Safety Considerations ie 5 Engineering Improvements m EE 6 ON AC HIT ASSIST AG Cares acces 6 RECEIVING YOUR INCUBATOR nsc eine ia 7 INSPECTING the SAD Laine 7 LAZ OA I 8 Recording Data Plate Information rinaldi PR E EUR RUFEN aS 9 R ference Sensor IC VIC CS ide 9 INSTALLATION eil iaia 10 ATOA CONA Silea 10 niece REA 10 Power Source Requirements iii 10 EC REA 11 Waler GAH ACHE rilanciare 11 Optional SWPK Water Purification K L iiiiiiiiii 12 IN LU RL 6 2 VOL Le priest 13 VC arie el te P 13 instal CU OI ECC ee 13 Connect to the Water Source iii 14 VDO TVA IT T 14 IEEE CORE EEA 15 HIM toniolo 15 Connect to the CO4 SUDPV iceealiabaiooneleoa 16 ACCESS FON 00 02 nno 17 GRAPHIC SYMBOLS Eea
23. NG IMPROVEMENTS Sheldon Manufacturing continually improves all of its products As a result engineering changes and improvements are made from time to time Therefore some changes modifications and improvements may not be covered in this manual If your unit s operating characteristics or appearance differs from those described in this manual please contact your Shel Lab dealer or distributor for assistance CONTACTING ASSISTANCE If you are unable to resolve a technical issue with the incubator please contact Sheldon Technical Support Phone hours for Technical Support are 6am 4 30pm Pacific Coast Time west coast of the United States UTC 8 Please have the following information ready when calling or emailing the model number and the serial number see page 9 EMAIL tech shellab com PHONE 1 800 322 4897 extension 4 or 503 640 3000 FAX 503 640 1366 Sheldon Manufacturing INC P O Box 627 Cornelius OR 97113 6 Page LAB RECEIVING YOUR INCUBATOR When an incubator leaves the factory safe delivery becomes the responsibility of the carrier Damage sustained during transit is not covered by the manufacturing defect warranty When you receive your SCO26H inspect it for concealed loss or damage to the interior and exterior If you find damage follow the carrier s procedure for claiming damage or loss INSPECTING THE SHIPMENT Before leaving the factory SCO26Hs are packaged in high quality shipping m
24. ads The door must close and latch fully Place the sensor probe of the temperature reference device as close as possible to the geometric center of the incubation chamber A thermocouple sensor probe sleeve may be taped to the shelving as long as the exposed copper end is 2 inches 5cm away from the shelf see Figure 19 An exposed sensor probe in direct Figure 18 Introducing a A i sensor probe through the contact with the shelving may experience heat sinking which can result in an access port inaccurate temperature reading R Stability Prior to a verification the chamber must operate humidified at its verification temperature set point for at least 1 hour with no fluctuations of 0 1 C or greater in order to be considered stabilized Failure to wait for stabilization will result in an Wait1Hour inaccurate verification For best results allow the unit undisturbed to run for 8 hours for example overnight after introducing and placing the probes but prior to performing the verifications Figure 19 Probe End 2 inches 5cm From Shelf Continued on next page Surface 32 Page LAB OPERATION CONTINUED Verifying the Temperature Display Accuracy 1 Once the chamber has stabilized with no fluctuations of 0 1 C or greater Reference Device compare the reference temperature device and chamber temperature display readings a Ifthe readings are the same or the difference between the two
25. amber CO value Procedure continued on next page 47 Page LAB USER MAINTENANCE CONTINUED Calibrate the CO Display Continued 4 Use the Up or Down arrows to adjust the current CO display value until it matches the reference device CO reading 5 After matching the display to the reference device wait 5 seconds a The display will cease flashing and store the corrected display value b The incubator will begin injecting CO2 or allow the current gas Wait 5 Seconds COncentration to decay in order to achieve the set point with the corrected display value 6 Allow the incubator to sit for at last 1 hour undisturbed to stabilize after it has achieved the corrected CO set point Wait 1 Hour a Failure to wait until the unit is fully stabilized will result in an inaccurate reading and calibration 7 Compare the reference device reading with the incubator CO display again a Ifthe reference device and the CO display readings are the same or the difference now falls within the range of your protocol the incubator is now calibrated for CO b Orsee next step Procedure continued on next page 48 Page Reference Device 58 Set CO BIET Set CO Set CO Reference Device Set CO BET LAB USER MAINTENANCE CONTINUED CO Calibration Continued 8 Reference Device C If the two readings are not the same and the difference falls outside the i
26. as input flow is vital for the incubator performance If connecting to a supply cylinder always use a two stage CO pressure regulator Be aware that some single stage regulators have 2 gauges Make certain your regulator is a two stage regulator 1 Attach the CO regulator to a medical grade CO cylinder if using a cylinder supply 2 Setthe cylinder regulator or wall source control to 15 20 Pounds per Square Inch psi Do not exceed 25 psi PSI Megapascals Kilopascals Bar 15 20 psi 0 108 0 137 Mpa 103 42 137 89 Kpa 1 03 1 378 bar Please see the Pressure Units Conversion table on page 51 in the Unit Specifications section for the formulas for converting psi into other units of pressure measurement 3 Remove the dust cover from the CO in port on the back of the incubator 4 Connect the gas tubing from to the regulator or wall source to the CO In Port on the back of the incubator di Figure 10 CO2 b Connect the clear tubing to the CO to Chamber port on the back of the to Chamber incubator a Connect the black tubing to the regulator or wall source 5 Do not initiate a flow of CO to the incubator at this time End of procedure 16 Page LAB INSTALLATION CONTINUED ACCESS PORT STOPPER The SCO26H Incubator is provided with an access port located on the left side of the unit The incubator is shipped with one 1 rubber access port stopper in the port The stopper should always
27. aterials to provide protection from transportation related damage Carefully inspect the shipping carton for damage Report any damage to the carrier service that delivered the incubator If the carton is not damaged open the carton and remove the contents Carefully check all packaging before discarding Save the shipping carton until you are certain that the unit and its accessories function properly The unit should come with an Installation and Operation Manual and a Certificate of Compliance Verify that the correct number of accessories are included Included Accessories 6 Shelves 24 Clips 4 Leveling Feet 1 Power Cord CO Tubing Kit d 995 00019 1250512 2700506 1800510 N 9710500 A rubber stopper comes installed in the access port on the right side of the incubator Note The SCO26H can be connected to a building CO gas system or a supply cylinder tank A cylinder regulator is not included with the SCO26H and must be purchased separately Please see the Accessories section on page 56 if you wish to order one from Shel Lab Figure 1 Optional CO Regulator 9740558 LAB 7 Page RECEIVING CONTINUED ORIENTATION PHOTO Water Inlet Back of Unit Pressure Compression Relief Back of Unit Fitting 1 4 inch Valve CO In Port 6 35mm fi a 2 Control Panel Back of Unit Door Gasket Power Inlet Fuse in Inlet Alarm Jacks Data Plate Incubation Chamber CO Sam
28. ber until a failed processor board and its temperature probe can be repaired or replaced 24 Page LAB OPERATION CONTINUED Note Setting up the incubator for use in a new workspace environment requires an 8 hour period for the unit to come up to and stabilize at temperature CO and RH levels prior to loading the incubation chamber with samples During this period the incubator must be powered continually supplied with water and CO and the chamber door closed Allowing time for stabilization protects samples It is also necessary for the optional temperature and CO2 display accuracy verification procedures PREPARING THE INCUBATOR FOR USE Perform the following steps and procedures to prepare the SCO26H for use each time it is installed in a new workspace environment 1 If you have not done so clean and disinfect the chamber if required by your laboratory protocol Please see page 15 in the Installation section 2 Verify that the side access port stopper is in place on the inside of the unit a Aclean and disinfected thermocouple probe for performing the optional temperature display accuracy verifications may be inserted through the access port at this time b Seethe optional Temperature Display Accuracy Verification procedure on page 32 for the correct placement of the thermocouple probe 3 Verify that the workspace power supply and the incubator power requirements listed on the data plate have been match
29. chamber accurate to within 0 1 C The arrow buttons can be used to adjust the temperature set point or place the display in its temperature calibration mode and then enter a display value correction Red LED alarm indicators marked High and Low illuminate whenever the temperature deviates by 1 C or greater from the current set point The yellow LED marked Mute illuminates whenever an audible deviation alarm is being muted See the Muting the Audible Temperature Alarm entry on page 28 of the Operations section for more information The green indicator labeled Heating Activated illuminates whenever the incubator temperature control system is heating the incubation chamber 20 Page LAB CONTROL PANEL OVERVIEW CONTINUED CO Display Labeled Set CO this display shows the concentration of CO in the incubation chamber as a percentage of the chamber atmosphere with a range of OFF 0 to 20 and an accuracy of 0 1 The display shows LO until the CO2 sensor registers a concentration in the chamber greater than 0 This may take several minutes when starting from a 0 concentration The UP DOWN arrow pad can be used to adjust the CO concentration set point and to mute audible gas deviation alarms The control can also place the CO display in its calibration mode and be used to enter a calibration adjustment Red LED alarm indicators marked High and Low illuminate whenever CO deviations of 1 or greater from the
30. ed a Seethe Power Source Requirement entry on page 10 4 Plug the power cord into the workspace supply outlet 5 Open the CO supply control or gas regulator so it supplies 15 20 psi to the incubator a lt can take up to 20 seconds for CO to reach the gas sensor in the SCO26H after the regulator is opened and the incubator is powered up 6 Place the Power switch in the on position Procedure continued on next page 25 Page LAB OPERATION CONTINUED Preparation Procedure Continued 7 Perform the following procedures in the Operation section a Set the Temperature Set Point page 27 b Set the CO Set Point page 29 c Set the Humidity Level page 31 d Optional Verify Temperature Display Accuracy page 32 e Optional Verify CO Display Accuracy page 34 f Set the Over Temperature Limit page 36 g Load the Chamber page 37 End of Preparation Procedure 26 Page LAB OPERATION CONTINUED SET THE TEMPERATURE SET POINT The unit comes from the factory with a set point of 37 C Perform the steps below to change the set point to the operational temperature you will be using for your incubation application Note The example below assumes adjusting the incubator set point from 35 C to a 37 C application temperature Set Temperature Set Point 1 Turn the OTL control clockwise to the maximum if not already set to max a This prevents the Safety cutoff system from interfering with th
31. ent room temperature 8 C The 8 degrees are generated primarily by the operation of the blower fan motor and resulting air compression in the duct spaces The chamber door is self heating to bolster the thermal uniformity and stability of the chamber and to minimize condensation on the glass viewing panel CO Atmosphere The same microprocessor board controls the gas concentration of CO in the chamber atmosphere by operating an internal injection solenoid valve connected to the gas input line The processor monitors CO concentration level in the incubator using an infrared sensor in the recirculation duct The sensor operates on the principle that a specific frequency set of infrared light is absorbed by CO The more CO present in the air stream from the chamber the more of that band of infrared is absorbed The sensor is only sensitive to CO2 so measurement accuracy is consistent regardless of the presence of other gasses in the incubator 22 Page LAB OPERATION CONTINUED The processor employs proportional integral derivative analytic feedback loop functions when measuring and controlling the CO2 concentration When the PID are active injection lengths are proportional to the difference between the measured concentration and the set point The frequency of injections is derived from the rate of change in the difference Integrator feedback slows the rate of injection as the concentration approaches the set point which
32. he difference falls within the range of your Set Temperature C protocol the incubator is now calibrated for temperature See the next step if the readings fail to match or fall outside of your i ERI protocol range Continued on next page 44 Page LAB USER MAINTENANCE CONTINUED Temperature Calibration Continued Reference Device or 8 Ifthe two readings are not the same and the difference still falls outside u e the acceptable range of your protocol repeat steps 3 7 up to two more E times a Three calibration attempts may be required to successfully calibrate Set Temperature C units that are more than 2 C out of calibration M If the temperature readings of the chamber and the reference device still fall outside your protocol after three calibration attempts contact your distributor or Sheldon Technical Support for assistance End of procedure LAB 45 Page USER MAINTENANCE CONTINUED CALIBRATE THE CO DISPLAY Note Performing a CO display calibration requires a gas reference device Please see the Reference Sensor Devices entry on page 9 for the device requirements CO calibrations are performed to match the incubator CO display to the actual gas concentration in the incubation chamber The actual concentration is supplied by a calibrated reference sensor device Calibrations compensate for drifts in the unit microprocessor controller as well as those caused by t
33. he natural material evolution of the IR CO sensor when continually exposed to a heated and humidified atmosphere with elevated CO2 concentrations Calibrate as often as required by your laboratory or production protocol or regulatory compliance schedule CO Supply The incubator must be powered the CO set point set and the chamber supplied with CO for at least two hours prior to the calibration Temperature The chamber temperature impacts the chamber CO concentration due to its influence on gas diffusion CO calibrations must be performed with the chamber fully heated and stable at your operational temperature set point A CO display calibration may be performed during a temperature calibration as long as the chamber door is not opened during either procedure Humidity Because humidity impacts CO concentration through its influence on temperature stability and uniformity the CO display should be calibrated with the chamber humidified Probes Connect a digital CO2 analyzer sample tube to the sample port located on the left side of the incubator near the top Stability Prior to a calibration the chamber must operate at its CO set point for at least 1 hour with no fluctuations of 0 1 or greater in order to be considered stabilized Failure to wait for stabilization will result in an inaccurate calibration and incubator display reading Figure 26 CO Sample Port For best results allow the unit to operate
34. ing either procedure The incubation chamber should be heated to and running at your application temperature as temperature influences gas diffusion in the chamber Humidity The incubator should be allowed to come up to humidity in the chamber prior to performing a CO verification Relative humidity affects CO distribution indirectly through its influence on the chamber atmosphere temperature Probes poue Co SAMPL Connect a CO reference device sample tube to the sample port located on the left PORT side of the incubator near the top Stability Prior to a verification the chamber must operate at its CO set point for at least 1 hour wit no fluctuations of 0 1 or greater in order to be considered stabilized Failure to wait for wait Hour stabilization will result in an inaccurate verification n Figure 20 CO Sample Port For best results allow the unit undisturbed to run for 8 hours for heat and humidity stability for example overnight after introducing and placing the probes but prior to performing the verifications A CO flow may be started to the chamber 2 hours prior to the start of the verification Continued on next page 34 Page LAB OPERATION CONTINUED Verifying the CO Display Accuracy 1 Once the chamber has stabilized with no fluctuations of 0 1 or greater Reference Device compare the gas reference device and chamber CO display readings a Ifthe readings are the sa
35. is procedure 2 Press and release either of the temperature arrows to activate the temperature set point adjustment mode OR a The temperature display will briefly flash the letters SP to Set Point Adjustment Mode indicate the Set Point is about to be displayed b The temperature display will then show the flashing adjustable temperature set point i Note The display will automatically exit the adjustment mode after 5 seconds of inactivity with the last shown set point value saved Initial Set Point 3 Use the Up or the Down arrow keys to adjust the set point to your application temperature O v New Set Point 4 Wait 5 seconds after entering the set point The display will stop flashing and the set point is now saved in i the controller a Qa b The chamber will now automatically heat or passively cool to match your set point C cage ncn c The display will revert to showing the current chamber air temperature Adjusting to Set Point See the Set the OTL procedure on page 36 for how to set the OTL system once the incubation chamber has stabilized at your application temperature set point or after you have performed any display verifications or calibrations End of Procedure LAB 2 Page OPERATION CONTINUED MUTING THE AUDIBLE TEMPERATURE ALARM An audible and visual high or low deviation alarm will activate if the incubation chamber temperature deviates by 1 C above or below the te
36. it was cleaned at the factory but not sterilized Additionally it may have been exposed to contaminants en route during shipping Cleaning and disinfecting the chamber and shelving prior to placing the unit in operation lowers the chance of microbiological contamination See the Cleaning and Disinfecting topic in the User Maintenance section see page 39 for more information on how to clean and disinfect the test soace chamber Remove all wrappings and coverings from shelving prior to cleaning and installation SHELVING INSTALLATION Perform the following steps to install the unit shelving 1 Install the shelf clips in the slots located on the shelf standard rails of the chamber interior a Squeeze each clip b Insert the top tab first c Then insert the bottom tab using a rocking motion 2 Hang the shelves from the clips Figure 8 Shelving Clip Installed LAB 15 Page INSTALLATION CONTINUED CONNECT TO THE CO SUPPLY Note Always use medical grade CO Use of non medical grade CO risks introducing contaminants into the chamber may damage the incubator and will void the manufacturing defect warranty Source CO To Chamber Figure 9 CO Source Connection The incubator may be connected to either a building CO supply source or a gas supply cylinder tank During normal operations the incubator uses only small quantities of CO to maintain the chamber gas concentration Precise regulation of the g
37. lease see page 12 in this manual for a complete description of the purification kit Part Number SWPK 56 Page LAB Sheldon Manufacturing Inc P O Box 627 Cornelius Oregon 97113 EMAIL tech Shellab com INTERNET http www Shellab com PHONE 1 800 322 4897 503 640 3000 FAX 503 640 1366
38. ll source or a gravity fed water reservoir that is regularly refilled pressurized source must be regulated to provide between 2 and 4 PSI 5 PSI is the cracking point of the pressure relief valve Shipping Cap _ gt Follow the steps below to connect the chamber to a water source 1 Optional Remove the plastic protective shipping cap from pressure relief value if it will be connected to a drain tube Water Inlet a he cap may be left on as a dust guard 2 Insert tubing from your laboratory water source into the 4 inch compression inlet on the back of the unit at the center top 3 Tighten the compression fitting to a snug fit wrist strength only Stabilizing a Use of two wrenches is recommended Wrench b Useone wrench to stabilize the body of the fitting This protects the fitting and the solenoid it is connected to from potential damage C Use the other wrench to tighten the fittings compression nut Tightening Wrench VAPOR TRAP DRAIN A condensate drain is located on the floor of the test space chamber The drain has a vapor trap that must be filled prior to putting the unit into operation Keeping the drain trap full is necessary for the unit to run at is performance specifications Pour approximately 72 cup 118ml water into the drain Ye Cup Figure 7 Condensate Drain 118ml Vapor Trap 14 Page LAB INSTALLATION CONTINUED INITIAL CLEANING The un
39. m before returning the unit to use Excess Condensate If condensation becomes excessive in the incubation chamber the following may help Note Note LAB Make sure items on the shelves are evenly spaced to allow for good airflow Ensure the chamber door is closing and latching properly Are frequent or lengthy chamber door openings causing significant temperature disruptions and chilling the chamber surfaces If so reduce the number of openings Is the incubator exposed to an external flow of cold air such as an air conditioning vent or a door to a cooler hallway or adjacent room Block or divert the air or reposition the unit Does the ambient humidity in the room exceed the stated operating range of 80 relative environmental humidity If so lower the room humidity Check the door gaskets for damage wear or signs of brittleness or dryness Arrange for replacement of the gaskets if damaged or excessively worn Periodically wipe down damp chamber surfaces to remove the condensate Rising or falling air pressure from weather will adjust the dew point up and down in small increments If the relative humidity in the test soace chamber is already near the dew point barometric fluctuations may push it across the dew point threshold Thin air at higher altitudes holds less humidity than the denser air found at or near sea level 3 Page OPERATION CONTINUED ACCESSORY COMPATIBILITY Make sure that any accessory e
40. m 78 1 x 66 0 x 143 5cm Access Port Diameter 1 45 inches 3 68 cm CAPACITY Cubic Feet Cubic Liters ish LAB UNIT SPECIFICATIONS CONTINUED CO2 Range Accuracy Recovery Time 0 20 Less than minutes TEMPERATURE Range Uniformity Stability Ambient 8 C to 60 C 0 5 C at 37 C 0 1 C 37 C POWER Model AC Voltage Amperage Frequency SCO26H KM PRESSURE CONVERSION CHART Conversion table for pressure units Se GU EM NE CAN NM 1 kPa 9 86923 X 10 3 NN 10010 01972X 10 1X 5038 X 102 7 50062 X 10 0 2953 X 102 9 86923 1 kgl am 9 80665 X 10 9 80665 X 10 2 1 42234 X 10 7 35559 X 10 2 8959 X 10 9 67841 X 107 2 953 10 9 86923 X 1071 69473 2 036 6 60457 X 10 1 mmHg i Hw Hi s p 9370 X 21131579 X 3 1 Torr 1 33322 X 10 1 1 33322 X 1 3 9370 X 1072 1 31579 X 10 1 inHg 3 3864 3 3864 X 1073 3 4531 X 10 4 3 3864 X 10 2 5400 X 1 NEM pe idi 1 03323 1 01325 1 46960 X 10 7 60000 X 10 2 9921 X 10 1 bar b 1 psi r 1 atm 1 01325 X 10 1 01325 X 10 LAB 51 Page UNIT SPECIFICATIONS CONTINUED Room Conditions 50 RH The relative humidity in the incubation chamber is affected by 20 C The chamber air temperature and heat transfers due to condensation The current ambient relative humidity and ambient air temperature The setting of the incubator RH control dial
41. mber sensor probes located at the rear of the chamber on the back wall 4 Clean all removable accessories and componenis 5 Clean and disinfect any attached sample tubing and replace if discoloring is present 6 Rinse the chamber surfaces and shelving with distilled water and wipe dry with a soft cloth Do not use deionized water 39 Page LAB USER MAINTENANCE CONTINUED Disinfecting Disinfect the incubation chamber on a regular basis For maximum effectiveness disinfection procedures are typically performed after cleaning Perform the following steps to manually disinfect the incubator 1 Turn the unit off Open all doors and carryout your laboratory clinical or production space disinfection protocol 2 Disinfect the incubation chamber using commercially available disinfectants that are non corrosive non abrasive and suitable for use on stainless steel surfaces If disinfecting external surfaces use disinfectants that will not damage painted metal or plastic Contact your local Site Safety Officer for detailed information on the disinfectants compatible with your cultivation or culturing applications 3 If permitted by your protocol remove all interior accessories shelving and other non attached items from the chamber when disinfecting 4 Disinfect all surfaces in the chamber making sure thoroughly disinfect the corners Exercise care to avoid damaging the sensor probes 5 Gas concentrations from evapo
42. me or the difference between the two 2 falls within the acceptable range of your protocol the display is accurately showing the chamber air temperature The CO Verification procedure is now complete See step 2 if a difference falls outside the acceptable range of your protocol 2 Ifa difference falls outside the acceptable range of your protocol perform a CO calibration to match the Set CO display to the actual chamber gas concentration a Please see page 46 in the User Maintenance section End of procedure LAB 35 Page OPERATION CONTINUED Note Test the OTL system at least once per year for functionality SET THE OVER TEMPERATURE LIMIT The incubator must be operating at your incubation application temperature and must be stable for at least one hour prior to setting the OTL Perform the following steps to set up the Over Temperature Limit system for use Example If you have not done so already turn the Set Over Temperature Limit control dial clockwise to the maximum position Turn the Over Temperature Limit control dial counterclockwise until the red Over Temp Limit Activated light illuminates Slowly turn the dial clockwise until the Over Temperature Limit Activated light turns off Stop turning the control Leave the OTL dial set slightly above the activation point The Over Temperature Limit is now set approximately 1 C above the current chamber air temperature End of Procedure
43. mperature set point The low deviation audible alarm has a delay of fifteen minutes This prevents the low alarm from sounding whenever the doors are opened causing a temporary drop in temperature 1 To mute an active high or low deviation alarm press and hold either the Up or Down arrow on the Temperature Control panel until the amber Mute LED illuminates and the audible alarm shuts off 2 The audible alarm component will remain muted for the duration of the current temperature deviation The visual alarm indicator will remain illuminated 3 Any new deviation of 1 C or greater will reactivate the audible alarm Figure 14 Low Alarm Muted AUTOMATIC DOOR CUTOFF Whenever the incubation chamber door is opened the unit stops the flow of CO into the chamber depowers the heater element and water vapor generator and ceases operation of the internal blower fan This limits the amount of CO and humidity released into the workspace around the incubator lt also prevents the heater from attempting to counteract the continual inflow of cooler air which would cause a significant heat spike once the door is closed Normal CO injections humidification heating and fan operation all resume automatically when the door is closed 28 Page LAB OPERATION CONTINUED SET THE CO SET POINT The incubator comes from the factory set to Off Set the CO set point to that of your application The gas supply must deliver 15 20
44. ns autoclaves and any device that produces significant radiant heat Heating and cooling ducts or other sources of fast moving air currents High traffic areas Direct sunlight POWER SOURCE REQUIREMENTS When selecting a location for the unit verify that each of the following requirements are satisfied 10 Page The wall power source must match the voltage and ampere requirements listed on the unit data plate Supplied voltage must not vary more than 10 from the data plate rating Damage to the unit may result if supplied voltage varies more than 10 The wall power source must be earth grounded The unit must be positioned so that all end users can quickly unplug the power cord in the event of an emergency The wall power source must conform to all national and local electrical codes Use a separate circuit to prevent loss of product due to overloading or circuit failure The unit is provided with a 250V 16amp 5x20mm fuse located in the power cord inlet These units are intended for 115V 50 60 Hz applications at 12 0 amps LAB INSTALLATION CONTINUED WATER DRAIN Condensate is drained out the back of the unit through a copper drain pipe with a 1 4 inch 6 35mm steel compression fitting The incubator must be positioned so that the drain empties to either a floor drain or a drain pan that laboratory personnel can regularly empty Never plug the drain WATER QUALITY REQUIREMENTS The incubator requires a continual c
45. nsor probe in the chamber allow the unit to operate and stabilize overnight and then conduct the calibration the next day Failure to humidify the chamber may result in an inaccurate calibration and display reading Figure 25 Probe End 2 inches 5cm From Shelf The chamber is considered stabilized when it has operated for 1 hour with no Surface fluctuations 0 1 C or greater Failure to wait for stabilization will result in an inaccurate Wait 1 Hour calibration Continued on next page 42 Page LAB USER MAINTENANCE CONTINUED Temperature Calibration 1 Once the chamber temperature has stabilized compare the reference Reference Device device and temperature display readings J amr os a Ifthe readings are the same or the difference between the two 2 falls within the acceptable range of your protocol the display is accurately showing the test soace chamber air Set Temperature C temperature The Temperature Calibration procedure is sessi ERT b Ifa difference fall outside of your protocol range advance to step 2 4 Y Reference Device 36 1 Set Temperature C ER 2 A display calibration adjustment must be entered to match the display to the reference device See next step 3 Place the display in its temperature calibration mode Press and hold both the UP and DOWN temperature arrow buttons simultaneously for approximately 5 seconds Set Temperature C Release the buttons when
46. nual may create a protection impairment by disabling or interfering with the unit s safety features This can result in injury or death Your SCO26H and its recommended accessories are designed and tested to meet strict safety requirements It is designed to connect to a power source using the specific power cord type shipped with the unit For continued safe operation of your incubator always follow basic safety precautions including e Always plug the unit power cord into an earth grounded electrical outlet that conforms to national and local electrical codes If the incubator is not grounded properly parts such as knobs and controls can conduct electricity and cause serious injury e Avoid damaging the power cord Do not bend it excessively step on it or place heavy objects on it A damaged cord can be a shock or fire hazard Never use a power cord if it is damaged e Always position the unit so that end users can quickly unplug it in the event of an emergency e Do not attempt to move the unit while in operation or before the unit has cooled e Use only approved accessories Do not modify system components Any alterations or modifications to your incubator can be dangerous and void your warranty e Follow all local ordinances in your area regarding the use of this incubator If you have any questions about local requirements please contact the appropriate agencies 5 Page LAB INTRODUCTION CONTINUED ENGINEERI
47. onnection to a water source while in operation Deionized water may not be used in SCO26H While DI water is useful in variety of laboratory applications it is an aggressive solvent that attacks most metals and will damage your unit Use of DI water in the incubator voids the manufacturing defects warranty Distilled water in the resistance range of 50K Ohm cm to 1M Ohm cm or a conductivity range of 20 0 uS cm to 1 0 uS cm is optimum for humidification and cleaning applications If distilled water is unavailable potable tap water that meets these standards is acceptable Filtering is recommended as tap water quality varies greatly by locality and may create mineral buildups in sensitive components such as solenoid injection valves and the vapor generator reservoir These accumulations decrease the efficiency of the humidification system and shorten its lifespan Additionally microbiological organisms in tap water may produce organic byproducts that strip protective coatings and accelerate rusting Water Usage When operating with the humidification control dial set to its mid point the chamber uses approximately 3 gallons every 24 hours Usage rates will vary with the incubation chamber temperature ambient humidity and the incubator humidification setting 11 Page LAB INSTALLATION CONTINUED Figure 4 Purification Kit OPTIONAL SWPK WATER PURIFICATION KIT Shel Lab offers an optional kit for purifying potable tap water
48. ose caused by the natural material evolution of the sensor probe in the humidified and heated chamber space Calibrate as often as required by your laboratory or production protocol or regulatory compliance schedule Humidity Always calibrate for temperature with the chamber humidified CO A CO calibration may be conducted simultaneously with a temperature calibration as long as the chamber door is not opened during either procedure Probes Reference device sensing probes may be run through the access port Carefully seat the port stopper over any probe wires Probes may also be introduced through the chamber door space Use non stick non marking tape to secure the wires and probe heads The door must close and latch fully Place the sensor probe of the temperature reference device inside as close as possible to the geometric center of the chamber A thermocouple sensor probe e sleeve may be taped to the shelving as long as the exposed copper end is 2 Figure 24 Introducing a inches 5cm away from the shelf see Figure 25 An exposed sensor probe in sensor probe through the direct contact with the shelving may experience heat sinking which can resultinan access port inaccurate temperature reading Stability Prior to a calibration the chamber should operate undisturbed and humidified at its application temperature set point for 8 hours in order to stabilize A common practice is to introduce and place the temperature se
49. ple Port RS232 Com Port a m Door Handle Door Latch View Window Access Port Figure 2 SCO26H ee ES Sensor Probes Main Temperature OTL Temperature RH96 Incubation Door Latch Shelf Standard Chamber Door Mounting Rail Back of Unit Chamber Drain Port inch Gasket 6 35mm x_i 8 Page LAB RECEIVING CONTINUED Manufactured in USA t SHELDON MANUFACTURING inc 30 26TH b a CORNELIUS OR 97113 www SHELLAB com 1 800 322 4897 RECORDING DATA PLATE INFORMATION Locate the data plate on the back of the unit near the power cord inlet The data plate contains the incubator model number and serial number Enter this information below for future reference Figure 3 Data Plate Date Plate Information Model Number Serial Number REFERENCE SENSOR DEVICES Reference sensor devices or a combined device must be purchased separately for performing display accuracy verifications or calibrations for temperature and CO concentration Reference devices must be accurate to at least 0 1 C and 0 1 CO and should be regularly calibrated preferably by a third party For best results use a digital device with wired connected temperature sensing probe that can be placed in the incubation chamber through the unit access port For example a wire thermocouple probe A gas analyzer should have sample tubing that can be connected to the incubator external CO sample
50. port Readings that avoid chamber door openings during verification and calibration eliminates subsequent waits for the chamber temperature and gas levels to re stabilize before proceeding This also allows temperature and gas verifications or calibrations to be performed simultaneously Select probes suitable for the application temperature you will be calibrating or verifying the incubator displays at Alcohol thermometers are insufficient for conducting accurate temperature verifications and calibrations Do not use a mercury thermometer Never place a mercury thermometer in the incubation chamber 9 Page LAB INSTALLATION AMBIENT CONDITIONS The SCO26H is intended for use indoors at room temperatures between 15 C and 30 C 59 F and 86 F at no greater than an ambient 80 Relative Humidity at 25 C 77 F Allow a minimum of 4 inches 10cm between the incubator and walls or partitions and 2 inches 5cm of clearance above the top of the incubator for unobstructed airflow Operating the unit outside these conditions may adversely affect its temperature range and stability For conditions outside of those listed above please contact your distributor to explore other unit options suited to your laboratory or production environment ENVIRONMENTAL FACTORS When selecting a location to install the incubator consider all environmental conditions that can affect unit temperature performance For example Proximity to ove
51. quipment used inside the incubation chamber can safely and effectively operate within your selected temperature RH and CO levels DATA OUTPUT CAPABILITIES The SCO26H incubator generates data outputs once per minute describing CO and humidity percentages as well as the temperature level using a pair of 4 through 20 milliamp bus analog output modules These outputs can be connected to a building management system BMS or other data monitoring and capture system through the use of three jack ports The ports accept standard audio jacks available from most electronics retailers These are also known as 4 inch 2 pole audio connectors or phono jacks Jacks are not included with incubator An RS232 serial port also outputs the same data once per minute as a digital logline Data Monitoring Systems Max Resistance For building management and other data monitoring or logging systems the maximum resistance of the current loop driven by either output from the 4 20mA module is 250 Ohms At higher loop resistances the current value will be erroneously low for parameter values near the top of the scale Jack Outputs Parameter Parameter Value at 4mA Parameter Value at 20mA TEMP CO2 HUMIDITY 0 CO 20 CO e e Bos oe Figure 21 Data Jack Ports RS232 Output Parameter Output Channel RS232 tempere L me 6 Mu Com Port Example logline for an RS232 output C1 37 0 C3 5 0 C4 95 38 Page LAB US
52. rating disinfecting agents can inhibit growth or cause metabolic symptoms in microbiological sample populations Make sure that chlorines amphyls quaternary ammonias or any other overtly volatile disinfecting agents have been rinsed or otherwise removed from the chamber surfaces prior to placing samples in the chamber MINIMIZING CONTAMINATION EXPOSURE The following are suggestions for minimizing exposure of the incubator chamber to potential contaminants e Maintain a high air quality in the laboratory workspaces around the incubator e Avoid placing the incubator near sources of air movement such as doors air vents or high traffic routes in the workspace e Minimize the number of times the incubator chamber door is opened during normal operations 40 Page LAB USER MAINTENANCE CONTINUED HEPA FILTER AND GAS LINES n Sheldon Manufacturing recommends replacing in line gas HEPA filter once per year or j when the filter is noticeably discolored HEPA filters are directional and must be installed facing in the correct direction The word IN is stamped on the rim of the filter assembly on the side that faces toward the gas supply 2800525 Gas lines should be replaced when cracking brittleness permanent kinking or other signs Figure 23 of damage are present Please see the Parts List on 55 Optional In Line Gas Filter STORAGE OF THE SCO26H INCUBATOR Perform the following steps if the incubator will
53. set point take place The yellow LED marked Mute illuminates whenever an audible deviation alarm is being muted See the Muting the Audible CO Alarm entry on page 30 of the Operations section for more information Marked CO Injecting this green indicator illuminates when the incubator is injecting CO into the incubation chamber Injections are accompanied by a clicking sound that is the CO solenoid opening and closing Set Relative Humidity This graduated control dial is used to set an approximate level of relative humidity RH in the incubation camber to prevent sample dehydration The green Humidity Injecting light illuminates whenever the humidity vapor generator is injecting water vapor into the chamber airstream The red Water Low light illuminates when the water level in the vapor generator reservoir is low below 0 88 liters While the light is illuminated the vapor generator will remain off in order to prevent overheating A continuous flow from a water source must be reestablished which will automatically refill the reservoir then turn off the light and restart vapor generator operations 21 Page LAB OPERATION THEORY OF OPERATION The SCO26H is engineered to provide a constant temperature CO incubation environment that can be humidified to prevent dehydration of sample media The unit can obtain a stable uniform temperature in its chamber ranging from the room temperature ambient 8 C up to 60
54. tem can be set to off by turning the set humidity dial to its lowest setting counter clockwise Water Usage When operating with the humidification control dial set to its mid point the chamber uses approximately 3 gallons every 24 hours Usage rates will vary by temperature ambient humidity and the humidification setting 31 Page LAB OPERATION CONTINUED TEMPERATURE ACCURACY VERIFICATION Note Performing a temperature accuracy verification requires a temperature reference device Please see the Reference Sensor Devices entry on page 9 for the device requirements Optional The SCO26H was calibrated at the factory at 37 C A verification of the display accuracy may be carried out when preparing the incubator for use if required by your laboratory or production protocol If an error between the actual and displayed temperatures is discovered perform a temperature calibration Please see the Calibrate Temperature Display procedure on page 42 in the User Maintenance section Humidity Always verify or calibrate for temperature with the chamber humidified CO2 A CO display verification may be performed concurrently with the temperature verification Probes Reference device sensing probes may be introduced through the access port M Carefully seat the port stopper over any probe wires Probes may also be introduced through the chamber door space Use non stick non marking tape to secure the wires and probe he
55. the temperature display shows the letters CO The display will begin flashing the current temperature display value Note f an arrow key is not pressed for five seconds the display will cease flashing and store the last displayed value as the new current chamber temperature value Continued on next page LAB 43 Page USER MAINTENANCE CONTINUED Temperature Calibration Continued Reference Device por E 38 4 Use the Up or Down arrows to adjust the current display temperature value until it matches the reference device temperature reading Set Temperature C EES 5 After correcting for the difference wait five b seconds Set Temperature C a The temperature display will cease flashing and store the corrected chamber display value b The incubator will now begin heating or passively cooling in order Wait 9 Seconds to reach the set point with the corrected display value Adjusting to Set Point 6 Allow the incubator sit for at last one 1 hour undisturbed to Set Temperature C stabilize after it has achieved the corrected temperature set point M Wait 1 Hour a Failure to wait until the incubation chamber is fully stabilized will result in an inaccurate reading Reference Device 7 Compare the reference device reading with the chamber temperature 3 mor l LI c display again a If the reference device and the chamber temperature display readings are the same or t
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