Thermo Sorvall T1 centrifuge
Contents
1. o o o O w o Oo oO lo jo o o o O vw o Oo Oo lo jo O o o Oo o Oo Oo jo jo o vw o o v oO jojo lo jo o o o o o o Oo o jo o sl SS B2 GB 2 je 5 5 AlDIn m DIG D D DI D S gt IBIS gt 5 lll a 2 lD a lala 5 5 o o o o 25 jojojo jo jo o o o o o o Oo o jo o o o o o O jo o o DO o o o o 5 lo o o jo jo o o o o o o o o o o oja o o 25 o Oo Oo DO O o 5 o o 25 o o Oo jo jo nin Ajo 2 2 2 Io D D o gre 2 o S 5 D s wo 8 ajg o S B LE o S e 8 e 3 5 5 a e lss e s s s s 2 2 5 5 58 o2 o o o o o 9 9 9s a m a ja a on a fos fos S 0 0 0 0 00 00 0 0 0 0 0 0 0 0 0 Butane S S S U 0 0 0 0 0 U 0 MS S MOS S 0 0 00 00 00 0 S 0 0 0 0 0 0 0 U 0 S 0 0 S S 0 0 0 0 0 0 0 S n Butanol S S U n Butyl Acetate Butylene U U U 0 0 0 0 0 U S 0 0 0 0 0 U U 0 0 0 0 0 0 0 0 U Butyl Chloride Butyric Acid S 0 0 0 0 0 0 0 U Mt o OO o oO OS O OD cl Le o O v O O O O O o vn CO Dm la lua O O OD ICH AIAN v en toi m O m co o Oo Vv Oo lolo 5 O Im 5 CO v Dm lala en Mm JO ca co en en v mv mw en jajn CO CO en vm Dm cn jo W a e ces mm eo m O 5 5 0 v en en en lajn jn r CID IO IO Oo JOJO low le o OO o Oo OS o OD oOo O o OO OS Oo2. Installation S41 Swing Out Rotor Cat No Description Capacity Max Min Max Radius Max Speed Max Tubeg Tube Ht mm rpm RCF xg mm mm 11210246 Swing out rotor 4 x 200 mL 61 4100 3026 11175611 Set of 4 bucket 200 mL 57 61 4100 3026 11175612 4 sealing lids 41193271 6 PP bottles with cap 200 mL 56 5 90 111 161 4100 3026 11174181 Set of 4 inserts 1x100 mL 45 80 121 159 4100 2990 11174501 Set of 4 inserts 1 x 50 mL round 35 56 123 158 4100 2970 11174528 Set of 4 inserts 1 x 50 mL conical 29 87 123 159 4100 2990 11174527 Set of 4 inserts 1 x 25 mL universal 25 86 125 158 4100 2970 11174502 Set of 4 inserts 3x20mL 22 80 120 159 4100 2990 11174537 Set of 4 inserts 3 x 15 mL conical 17 5 89 124 156 4100 2930 11174183 Set of 4 inserts 7x15 mL nalgene 17 60 117 156 4100 2930 11210419 Set of 4 inserts 3 x 15 mL urine 17 159 4100 2990 11210523 Set of 4 inserts 3 x 15 mL Vac 17 5 159 4100 2990 11174509 Set of 4 inserts 3x 12 mL graienr flat 17 5 79 126 159 4100 2990 11174503 Set of 4 inserts 5x10 mL Vac 16 58 121 159 4100 2990 11174534 Set of 4 inserts 5x 5 10 mL flat 17 42 118 159 4100 2990 11210604 Set of 4 inserts 7x5 7 mL 13 159 4100 2990 11174505 Set of 4 inserts 9x5 mL Vac 13 5 60 118 159 4100 2990 11174533 Set of 4 inserts 5x5 mL flat 17 42 159 4100 2990 11174506 Set of 4 inserts 12 x 3 5 mL round bottom 12 42 116 58 4100 2970 11174508 Set of 4 inserts 6 x 1 5 2 mL 1 45 120 158 4100 2970 T20 High Throughput Rotor Cat No Description Capacit
3. Pay special attention to the following e Installation of the unit proper ventilation leveling of the centrifuge rigidity and stability of the support e Rotor installation verify that the rotor is locked in position before use e Cleaning of the accessories and of the rotor chamber is particularly necessary when corrosive products are present in the samples saline acids bases e Load balancing If any dangerous event occurs keep yourself far away from the instrument and switch off the main power source A view port located on the lid allows the measurement of the actual speed by a phototachometer Once a year check for correct r p m readout and speed control setting which should be within 100 r p m of the actual The T1 centrifuge and its associated rotors offer a high level of RCF performance In some cases the maximum possible RCF will exceed the one sustainable by the sample container The rotor RCF performance displayed is the maximum available in the rotor pockets Depending on the sample containers or on other application needs the maximum RCF which must be used is to be determined by the operator The centrifuge is not explosion proof Using this centrifuge with explosive samples is entirely at the users own risk Do not under any circumstances use the centrifuge in an explosive environment A Due to the action of the turbulence created in the bowl a centrifuge is an aerosol generator A ventilated
4. Limitations of Use This centrifuge conforms to the IEC 1010 2 020 international standard which specifies particular safety requirements for laboratory centrifuges According to the standard warnings the operator must respect the following precautions e Marka boundary area of 30 cm around the centrifuge e No person or hazardous material can stay within this boundary longer than necessary for operational reasons while the centrifuge is operating e An emergency switch for disconnection of the mains in case of malfunction has to be available and familiar to all the persons operating the centrifuge This switch has to be remote from the laboratory centrifuge preferably outside the room in which the centrifuge is located or adjacent to the exit from that room Moving or shifting the machine during centrifugation is dangerous e Using the centrifuge with rotating equipment showing evidence of corrosion and wear marks manipulating and or tampering with the electronic and mechanical parts are dangerous operations e Never try to bypass the lid lock safety while the rotor is spinning e Do not try to open the lid until the display returns to the stand by mode e Only use a correctly grounded power source e Use only rotors and accessories designed for use in these centrifuges A T1 RT1 5 1 Chapter 5 Hazard Precautions and Limitations of Use 5 2 T1 RT1 Speed Control Operational Limitations Aerosol Risks
5. the middle display will always show time The user cannot change the display to show the given radius value during a run Most set parameters can be changed during a run when the selected program is not locked Set speed can be changed by selecting speed as the control parameter for the upper display then by pushing the up or down arrow Set run time can be changed by pressing the up or down arrow corresponding to the middle display Set deceleration profile can be changed by first selecting the deceleration profile and then using the up down arrows When the stop button is pressed or when the run timer reaches zero the centrifuge will begin to decelerate the rotor At this point the run timer will stop and the middle display will begin to show elapsed braking time The upper display will show either actual speed or actual maximum RCF values whichever is currently selected The lower display shows the given deceleration profile and the LED corresponding to the acceleration button is illuminated At the end ofa run after the rotor has been decelerated to rest the centrifuge will produce an audible beep and the middle display will show the word End Pressing any button or opening the lid will bring the displays back to their normal at rest configuration display of set parameters Thermo Fisher Scientific Thermo Fisher Scientific Error Codes Chapter 4 Instructions for Use Error code
6. 0 S S 0 0 0 0 0 0 0 S 0 0 0 0 0 0 0 0 Sd Sd S Sd S 00 0 0 0 S 0 0 0 0 0 0 0 S S 0 0 0 0 0 0 U 0 0 0 0 0 0 S U 0 0 Paraffin S 0 0 0 0 0 0 0 U Pentane o o o o o v lO jo lo o o v o oO 5 jojo jo o Oo c mw o m 5 OD lo o o v t jo jm N O jo o o 5 o O v lO jo lo Z B o EB E 5151515 Dj gt lO 5 o Vv 5 gt gt BB ZB E S gt EZ S ESCH 5 gt o o o o o 5 jojo jo o lolo lolo O jojo jo o Oo v o O vn lO jo lo o o 53 o o 5 lolo jo o o o ojo oo O jo o o o o O Oo O jo jo o o o o Oo 5 O O jo a lt D lt jon ja lo jo jo as c c D d e le l e oe o Izl l S ZIT SES KE e 2 2 2 2 2 2 2 slslslse 8 6 6 5 85 5 O e a gt a E Bm AS AS s AS AS AS A 0 S S 0 0 S S 0 0 S S S S S 0 S U 0 0 0 0 8 0 0 Phenyl Ethyl Alcohol S 5 0 S S S 0 MS SU Phosphoric Acid 10 AID Oo jojojo lo lo o OD w miooo jo miooo jo gt jojojojojm mio gt injojo ajd r o co Di in en gt 2 ja ID yo co 2 5 o o ojojojojo jm o o oj o o o co o o jojo gt pojojojojo gt jojojojojm jojojo jo jo gt pojojojojo Ojo o 15135 E 2 D SIE E a 2 1215 lt 2 ja o SEE o St ed HE 2 2 So 2 4 s s ge S S S Ss
7. 52 5 lo o o o o lio 2 2 o o Oo o oO lo lo ce o o o o o we o o a gt O O O v m O D Zlo p D o g 2 2 5 5 2 22 oia S S ZlS lB l oOI I R IN N S O OJO 5 gt o E E El Soo S5 lt 2 2 jols e BS SOS o o 2 OI gt gt gt gt gt Am E E O 22 9 PY 0 ien rer kel gt ELI ELI ELI II ELI ELI II HI II LH S SMO 000 St S S 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Ethyl Lactate M S S MO 000 0 0 0 0 Ethyl Malonate Fatty Acids S 0 0 0 0 0 0 0 Sd Sd Sd Sd S 00 0 0 0 S 0 S 0 U S S S 0 0 MS S S S M 0 0 0 0 0 0 0 S U Ferric Chloride Ferric Nitrate SM 0 0 0 0 0 0 St 0 S S Sd St S SM S S 0 0 0 0 0 0 S S 0 S 0 0 0 0 0 0 0 S M 0 0 0 0 0 0 0 S S 0 0 0 0 U UMMU Ferric Sulphate Ficoll Paque Fluorine 0 0 S S U 00000 S 5 Mt 0 0 0 Mt S 0 S S U Fluorhydric Acid 10 Fluorhydric Acid 50 M U SSMUS S 0 0 S U U U Thermo Fisher Scientific T1 RT1 6 6 Chapter 6 Service and Maintenance Material BN CAB CN DL KY NO NY PA PC PE PP Chemical PPCO Formaldehyde 20 S Sd Formaldehyde 4096 S S Formaldehyde 50 S Sd Formic Acid 10096 S M Freon TF Sd Sd Fuel Oil Sd Glucose Glutaraldehyde Glycerine Glycerol Heptane Hexane Hydrazine Hydrochloric Acid 596 Hydrochloric Acid 37 96 Hydrochlo
8. Canada or Latin America Tel 1 866 213 17 90 Fax 1 866 984 37 66 China Shanghai Tel 86 21 68 65 45 88 Fax 86 21 6445 7907 86 21 6445 7830 India Mumbai Tel 91 22 5542 9494 Fax 91 22 5542 9495 Japan Tel 81 454 53 9220 Fax 81 454 53 9222 Other Asia Pacific Countries Tel 852 28 85 46 13 Austria Tel 43 1 80 14 00 France Tel 33 1 69 18 77 77 33 2 28 03 20 00 Belgium Tel 32 24 82 30 30 Germany Tel 49 61 84 90 69 40 Fax 49 61 03 40 81 012 Finland Tel 35 89 32 91 00 Thermo Fisher Scientific Italy Tel 39 02 95 05 91 Netherlands Tel 31 7 65 71 44 40 Russia Cis Tel 7 09 52 25 11 15 Spain and Portugal Tel 34 9 32 23 31 54 Switzerland Tel 41 1 4 54 12 12 United Kingdom and Ireland Tel 44 87 06 09 92 03 Other Europe Middle East or Africa Tel 49 61 8490 6940 Tel 33 2 2803 2000 In Other Countries Contact your local THERMO products distributor or agent E mail Technical Service Representative for products at techsupport led asheville thermo com Visit our web site at www thermo com T1 RT1 vii viii T1 RT1 Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Contents Use and PUM Ct iccirco inca c Solon 1 1 Us Manta 1 1 cs OMM DC 1 1 IRI e M 1 1 Refrigeration and Heating Sy t m ass o o rs 1 2 IR 1 3 Safety Interlock st suman ove o e to eme 1 3 Imbalance Detection Systtin s
9. OS O o o o o o lo Oo O O O 5 o OS vw Oo o OS 5 OD JoJo oo oO O lo 5 OD oo o S OS OS Oo OS O OD cl bd x 53 D fS E 2 eb ww D olg o 2 3 5 E e I5 S B melle 2 lo SIE SE 2 e 2 85 GS S l 2 Sla o s 8 53 l e E m O E 9 DIES C5 le l SEES EEE 3 3 3 3 3 3 5 5 5 5 o o o o o o o 2 22 GIS OO O O O jojo Jo S 0 0 0 0 0 0 St 0 U U U M 0 0 0 0 0 0 0 S Calcium Sulphate Carbazole U 00000 S U S U 0 0 0 0 0 0 0 0 S 0 0 0 0 0 U S 0 0 0 0 0 0 0 U Carbon Sulphide S S S UM 0 0 0 0 0 M U U S SM 5 0 S 0 0 0 0 0 0 0 0 V St M 0 0 0 0 0 0 0 St X Carbon Tetrachloride U Cedarwood Essence Chlorine dry 0 0 0 0 0 0 0 0 0 0 0 0 St Sd St Mt Sd St U 0 0 0 0 0 0 0 Mt Chlorine moist o O O vm ODO OD cs o o v m DO o 5 o Oo o zio o cc o oO v O JO o o o m o III 3 e 0 e a vn 2 53 5 jv jm lc mn In 2 2 5 mmm j po a jon 23 5 5 jv a o o O 2 O DO OD O o Oo O DO OD OD vm O o IO v mw O JO o Oo 5 o jo C Oo oO OD ID lc o lO 5 0 0 D SO DS IB DD 5 2 o o Ix 5 5 o o o le lo SE 2 8 le i5 eot Oe o 2 8 o mE 218 18 28 3 8 o R 2 E l lala 21812 ale
10. Scientific Chapter 6 Service and Maintenance Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Xylene SOU P DM S O VU VU U U SS S SS S Zinc Chloride MS S 0 0 0 0 SS SS SS MS S S S Zinc Hydrosulphite U 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Zinc Sulphate U 0 0 0 0 0 0 0 Sd 0 S Sd 0 00 0 0 0 This table is intended as a guide only because of the difficulty in guantifying cross checking and monitoring the results under different conditions of temperature pressure and purity relating to the solvents and samples dissolved therein It is strongly recommended that you carry out your own trials particularly before protracted work periods 6 10 T1 RT1 Thermo Fisher Scientific Thermo Fisher Scientific Certificate of Decontamination and Cleaning Read carefully the instructions below before sending an instrument or parts of it to Thermo Service Dept Jouan S A or to any Authorized Technical Assistance Service A Mr Mrs name sss Establishment PVG A USI eer Address Post Zipcode s Bils ass Zasas 9s S as ETE State Country Declares the cleaning and decontamination of the following SG en E Serial N aem H dite Serial N A M Serial N Accessory DESCIIDUON eege tert pul supeo oro deeg Serial N ACCESSO Y DEN e rd no Serial N N
11. The centrifuge will tolerate 10 gr of imbalance with the 4 x 280 mL rotor equipped with standard buckets Carefully balance the sample load to avoid actuating the imbalance detection system A Relative Centrifugal Force RCF at the circumference of a rotor and bucket combination is directly proportional to the speed r p m and radius of the rotor Therefore a greater r p m and or a larger radius produces a greater RCF and improved faster separation of substances The centrifuge control system carries out and displays the results of all calculations related to speed radius and RCF Note The value introduced for the radius can be adjusted to allow for position within the tube such as at a boundary Maximum radii are quoted in the specifications tables Use of improper radius will adjust the speed setting automatically applying the wrong RCE A Legend R radius in millimetres N speed in r p m 1000 RCF gravitational acceleration g M add to memory MR memory recall Note To calculate actual results press the keys on a pocket calculator in the order shown A Thermo Fisher Scientific Chapter 1 Use and Function Primary calculations Key sequence RCF x g 1118RN Nx x1118xR Speed rp m 946 RCF RCF R V x 946 R Radius mm RCF Nx x1 118 M RCF MR 1 118 N Transformations Key sequence To determine actual g achieved at a different speed RCF2 RCF1 M N2 N
12. Tubes RE 6 4 T1 RT1 ix User Manual Warning Symbols S Of A Description Thermo Fisher Scientific Chapter 1 Use and Function The user manual is part of the centrifuge and contains important information for your safety and for the best use of the equipment Always keep the manual close to the appliance and in a safe place so that it is always available Thermo strongly recommends that all users read this manual carefully The following symbols are provided to help the operator take advantage of the protection afforded by the equipment and to warn of potential danger On the main switch allows centrifuge to operate A On the main switch disconnects the centrifuge from the main power supply A Only authorized personnel can touch the parts close to this symbol and in any case only after switching off the main power supply Coming in contact with high voltages could cause severe injuries A In this manual this symbol means that you will find important information for safety which if unobserved could result in damage to the appliance and or harm to the operator A In this manual this symbol means that you will find important information about minimizing biological risk if unobserved the result could be harm to the operator A The T1 and RTI centrifuges are designed for laboratory use They will separate the components of fluids into layers of varying density by subjecting them to high forces Centrifu
13. U 0 S 00 U0 S S S 00 8 SS 0 Aluminum Chloride 0 0 SS 0 350 SS SS S 0 US 5 S 0 Aluminum Fluoride 0 0 0 0 0 SOS SUS 0 0 8 0 S 0 Aluminum Hydroxide S 0 0 0 0 0 0 0 S M Sd 8 S 0 0 0 0 0 Aluminum Nitrate MO 0 0 0 0 0 085 0 0 8 0 0 0 0 0 0 Aluminum Sulphate S 0 0 0 0 0 0 0 Sd 0 8 Sd 0 0 0 0 0 0 Amino Acids S 0 0 0 0 0 0 0 SS SS 50000 0 Ammonia S 0 0 0 0 0 0 0 SUS 838010000 0 Ammonium Acetate 0 0 0 0 0 0 0 0 SS SS 0 0 85 S S 0 Ammonium Carbonate S US S 0 S 0 SS US SS SS S S 0 Ammonium Chloride MO 0 0 0 0 0 O St O St St 0 0 0 0 0 0 Ammonium Hydroxide 10 O SU 00 0 0 SDUS DS SS SS S Ammonium Hydroxide conc O U U 0 0 0 0 SDUS DOS S S SU Ammonium Oxalate 0 0 0 0 0 0 0 0S SS Sd S 00 0 0 0 Ammonium Phosphate U 0 0 0 0 0 0 0 SMS S 0 0 0 0 0 0 Ammonium Sulphate S S 0 0 U 0 0 SS SS 8505 S S S 0 Ammonium Sulphide 0 0 0 0 0 8 0 0 SU 0 S 0 0 S 0 0 0 n Amyl Acetate S 0 0 0 0 0 0 O Sd U Sd Sd U O 0 0 0 O Amyl Alcohol SM U OS 0 0 SS SS S 0 0 S SSM Amy Chloride S 0 0 0 0 0 0 O U U U U U 0 0 0 0 0 Aniline S 0 0 0 0 S 0 0 U OS MO 0 S S S 0 6 4 T1 RT1 Thermo Fisher Scientific Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO 0 00 U Chemical U U 0 0 S S SM 0 8 000000 0 S 0 0 0 0 0 0 U U 0 U 0 0 0 0 0 0 0 S U U Aqua Regia Barium Chloride U 0 0 0 0 0 0 0 Barium Hydroxide Barium Sulphate S 0 0 0 0 0 0 St 0 S 0 0 0 0 0 0 0 S
14. been pierced item N 85241772 J ici avec l outil Y Pierce here A with tool e e 7 A y Thermo Fisher Scientific T1 RT1 3 4 Chapter 3 Installation Performance and Accessories Note The performance figures indicated below are those of the rotors Their use at these speeds necessitates that the sample containers can support the corresponding forces A T41 High Throughput Swing Out Rotor Cat No Description Container Capacity Capacity Max ube Max tube Radius Max speed Max RCF Volume Insert Rotor 8 mm ht mm mm 11210435 T41 rotating head 4100 3176 11210436 Set of 4 standard buckets TI 175712 Set of 4 sealing lids for 11175711 11175763 175763 Set of 4 buckets 5 7 mL vacu 15 60 13 5 65 4100 3101 175752 Set of 4 buckets 50 mL con 4 16 33 120 69 4100 3176 175759 Set of 4 buckets 50 mL con 3 12 33 120 69 4100 3176 Self Standing 175713 Set of 4 inserts 280 mL 4 64 113 161 4100 3026 175714 Set of 4 inserts 250 ml flat 4 61 112 161 4100 3026 175715 Set of 4 inserts 200 mL flat 4 55 110 156 4100 2932 175716 Set of 4 inserts 175 ml 4 60 120 166 4100 3120 11175719 Set of 4 inserts 100 mL 4 38 123 161 4100 3026 11175720 Set of 4 inserts 65 mL 2 8 34 104 156 4100 2982 85540175 Set of 4 insert 50 ml skirted 4 29 63 4100 3063 175722 Set of 4 inserts 50 mL con 2 8 29 114 34 4100 2518 21034
15. followed by distilled water and then dried completely Thermo makes no claims as to the effectiveness of proprietary brands of decontaminating solutions If the main circuit breaker of the centrifuge cuts off the power to the instrument do not attempt to switch it on before a Thermo Service representative has checked over the unit To remove the rotor from the motor shaft Press the unlocking device stored in the clip on the side of the centrifuge into the centre of the rotor and then lift out the rotor using both hands Replace the device in the clip Thermo Fisher Scientific Trunnion Lubrification Thermo Fisher Scientific Chapter 6 Service and Maintenance Sealed rotors may be removed with the lids still in position and moved to a safety cabinet for manipulation of hazardous material This operation is necessary to allow the buckets to swing freely Clean the trunnions regularly with a dry wipe as well as the part of the bucket that rotates on the trunnions Then put a very small quantity of grease on the curved face of the trunnion Do not apply too much grease because it will eventually coat the bowl of the centrifuge as centrifugal force pulls the grease from the trunnions If the centrifuge is having imbalance problems try this operation before calling Thermo service In many cases imbalance problems arise due to the fact that poorly maintained bucket trunnion interfaces prevent the buckets from swinging free
16. gt gt 5 lu 5 VE ET s E SIS 2 ol loja la ja jam o o In o o o 53 o o 53 jojo lolo o o Iv o o o oO o o o jojo lolo o o o o o Oo lo o o DO O OD O o o vn o Oo Oo v oO O 5 o o o S o o 23 o o o Iv o o 53 O DO JoJo o o 3 oo o lv o o 53 jojojo gt o o 23 o OD Do Iv o o Do OD o lo gt o o O O v INDIO es cs w D ES LLI ES oo 2313 5 5 e 8 8 E EIE oO s s e Ss 5 o a s 2 ojoje 5 2 RO O 5 pIe a D S S MEA 2 2 e 5 E BE 2 2 5 9 5 E e ejyejejejo o CH el bf z z o sslslol l l l 5 5 5 5 5 SSL lc lc 5 5 5 5 e ej e Oo O e e e e e e o o E E E S l ela lO CG lA A A A A A A A lA 0 0 0 U 00 U 00 0 0 0 0 S 0 0 0 0 Diphenyloxide S S 0 0 0 0 0 S S 0 0 0 0 0 00 0 8 Sd S Dipropylene Glycol Distilled Water Ethanol 50 S S S S S 05 SS SS S S S S S S S S S S S S U SM S S S M S c5l o o o o o we o o coc o oO o o o Vv o an O O Oo O O o O 5 coc o oO o o o Vv o nlo o o o o o o jo jo molina qn o 5 5 5 l 5 r 5 o c d l OO P 5 5 5 lo gt v S n IS le Nil ln 2 5 o o o oO gt jo lo mn o o o o o o Oo O jo vla o o o o o lm jo lo e lO oO OS SO 0 MD IO lO
17. ol ol ol ol ol YM al ol ol wm ao ol Z al Z ol o Z olol ol olol o ol ol So o c O toi ajaj Zi c cp al a E a z a a al a al al aal olaja a Lg S v al SPC clala o ol z ol alal ajl a a a olal olal gL x zIclolx o ajiz olo Thermo Fisher Scientific T1 RT1 6 7 Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO Chemical o o O o o jo o o O v o o jo o v O v o o jo o v O v o o jo o a o a v lolo O lu jo jo jo gt a ln 5 5 gt s AIM N S 55 gt coe A 5 2 AlAIN AIS DI D o 5 Oo O O o o o v oO ICH jo o o o v oO ICH jo o 53 o 53 5 IEN ICH o vw o S jojojo o v oO 5 5 O lO o 53 o 5 5 IEN ICH 2 2 Hijo o S 8 8 Zelle Il NE SS 9 EB S s5s5s55sStsg olo x la cm Plam lt lt lt o olo lo o Lo IS x ad edel Mell Mell sg 5 5 2 z z 2 Z Z Le IZ S Sd 0 0 0 0 0 0 0 0 0 0 00 0 8 Sd 5 S 0 0 0 0 0 0 0 0 Octane 0 S 0 0 0 0 0 0 0 S Octyl Alcohol Oleic Acid S SSS SS S S SS 0 Ss S 0 0 0 0 0 0 0 Mt MMS S 0 U M M Mt MO 0 0 0 0 S S S S Orange Essence Oxalic Acid M S SS SS S S S S S 0 S S S 500000 S 0 0 0 0 0 0 0 S Oxygenated Water 20 Oxygenated Water 50 Oxygenated Water 90 Ozone S S 0 0 0 0 0 S S 0 0 0 0 0 S 0 0 0 0 0 0
18. radius in millimeters To increase decrease the centrifugal radius press on the correspon ding up down arrows This parameter can only be viewed and set while the centrifuge is at rest It is essential that this value be correct for the RCF control display function to be accurate The cor rect value of the centrifugal radius depends on the combination of rotor buckets adapters being used Thermo Fisher Scientific Function Set acceleration profile Chapter 4 Instructions for Use Keystroke sequence and description When the button 8 is pressed the lower display 17 shows the number corresponding to the acceleration profile currently registered 1 to 5 5 being the fastest To change the value press the corresponding up down arrows Set deceleration profile When the button 9 is pressed the lower display 17 shows the number corresponding to the deceleration profile currently registered 0 The rotor from 2000 rpm will coast to a stop 1 to 5 5 being the fastest To change the value press the corresponding up down arrows Set temperature R only To increase decrease the temperature set values press on the corresponding up down arrows To pre program the centrifuge There are available memory locations for pre programmed protocols Each corresponds to one of the poor memory buttons 1 4 To enter save a program into one of the locations press the memory button corresponding to the desired progra
19. regulations allow the centrifuge lid opening when rotor speed is below a certain defined level very few rpm This conditions is therefore not to be considered a fault or riscky for the operator A The centrifuge will not operate until the lid is closed and latched in place The lid remains latched until the rotor stops spinning The lid access lamp indicates when the rotor has stopped consequently the handle on the right hand side of the centrifuge can be used to open the lid If a power failure occurs access to the samples in the centrifuge is possible via a manual lid opening procedure see appropriate paragraph For this operation a special tool supplied with the centrifuge is required Bypass the interlock system only under emergency conditions as the rotor could still be rotating A The T1 centrifuge is equipped with a load imbalance detector In case of excessive imbalance the load imbalance indicator LED is illuminated and the brake is applied immediately The rotor will be T1 RT1 1 3 Chapter 1 Use and Function 14 Relative Centrifugal T1 RT1 Force Configuration formule decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides If the message persists despite your efforts to balance the load call your Thermo Service representative Note Imbalance tolerance depends upon the rotor in use
20. 0000 9838 11174716 Set of 6 inserts 1x15 mL con 17 93 10000 10397 11174717 Set of 6 inserts 2 x 10 mL 16 83 10000 9279 AC 50 10A High Speed Rotor Cat No Description Capacity Max Tube Y Radius Max Speed Max Angle mm mm rpm RCF xg deg 11175754 Angle rotor 6 x 50 mL con round 29 5 x 118 112 10000 12520 40 11174606 Set of 4 adapters 1 x 15 mL conical 175x111 112 10000 12520 11177378 Set of 4 adapters 1x10mL 17 x 75 91 10000 10174 11174599 Set of 8 adapters 1x30 32 mL 26 105 10000 11738 11210577 Set of 6 adapters 1x50 mL rnd 28 5 10000 Set of 6 conical to round adapter cushions supplied FIBERLite F15J High Speed Rotors Cat No Description Capacity Max Tube Y Radius Max Speed Max Angle mm mm rpm RCF xg deg 11210850 Angle rotor 8 x 50 mL conical 29 5 x 118 104 10000 11626 25 75100378 Set of 2 adapters 1 x 15 mL conical 175x111 104 10000 12520 AC 2 14 Microvolume Rotors Cat No Description Capacity Max Tube Y Radius Max Speed Max Angle mm mm rpm RCF xg deg 11175741 Sealed angle rotor 24x 1 5 mL 1x39 84 14600 20016 45 11175642 Spare sealing lid FIBERLite Micro Cat No Description Capacity Max Tube Y Radius Max Speed Max Angle mm mm rpm RCF xg deg 41174928 Set of 20 adapters 1 x 500 800 uL 8 14600 23113 45 11174631 Set of 20 adapters 1 x 200 uL PCR 65 41174938 Set of 20 adapters 1 x 250 400 700 uL 6 Suitable for the AC 2 14 rotor DC 6 11 Microvolume Drum Rotor Cat No Description Capacity M
21. 8 Set of 4 inserts 50 ml con 3 12 29 114 34 4100 2518 175721 Set of 4 inserts 50 mL round 4 16 28 5 106 132 4100 2481 175742 Set of 4 inserts AMICON 1 4 28 135 60 4100 3007 Centriprep 11175723 Set of 4 inserts 30 mL round 5 20 25 5 102 157 4100 2951 11175724 Set of 4 inserts 25 mL 5 20 24 104 155 4100 2913 11175725 Set of 4 inserts 25 mL Corex 4 16 24 107 155 4100 2913 11175726 Set of 4 inserts 16 mL round 9 36 18 107 160 4100 3007 11175727 Set of 4 inserts 10 mL vacu 12 48 17 107 160 4100 3007 11175738 Set of 4 inserts 15 mL vacu 4 16 16 124 163 4100 3063 175729 Set of 4 inserts 15 mL urine 7 28 17 107 61 4100 3026 175730 Set of 4 inserts 15 mL con 4 16 17 119 63 4100 3063 175731 Setof 4 inserts 5 mL con CN 207 17 116 63 4100 3063 175747 Set of 4 inserts 4 mL 4 16 15 5 121 63 4100 3063 Corning round 175732 Set of 4 inserts 3 mL 2 48 16 115 60 4100 3007 11175728 Set of 4 inserts 10 mL 9 36 16 112 160 4100 3007 11175733 Set of 4 inserts 8mL 19 76 12 104 161 4100 3026 11175740 Set of 4 inserts 5 7 mL vacu 12 48 12 5 75 160 4100 3007 11175734 Set of 4 inserts 5 mL RIA 19 76 13 104 161 4100 3026 11175735 Set of 4 inserts 3 mL 19 76 11 106 161 4100 3026 11175736 Set of 4 inserts 1 5 2 mL 13 52 10 42 159 4100 2988 in open buckets only 3 5 T1 RT1 Thermo Fisher Scientific Chapter 3
22. GD GD ol ol D O OD OD WM wm GD Oo J Z oj c z c CP KC CP GD DF OD DJL OJ AP os ala a a Z o ZS oO jojojejojojec oe Rar om earl jojo opojcjojojo opjojojojoje cjojo jo c lt c jcjojojojojojojojo ojojojcjojojo ol o O c oj oj S oc oc ol ol ol ol ol ol ol sol ol oo ol clol oj al o c lt ojecjojojojojojo ojo ojojojojoj o o o ololulolo z o z o z olol olol ol ol ol os ol ol ol ol ojo moje jao cjcj cjcjojojojojojojojo ol ol oj c ol oj olol olal olol zl olal olal DG DGD DG OD M DP OD GD opjojojo ojo AD D O DO DY WN OD DW GD D O DG DO WB GD D O GD GO oal olojo ojojojojojojo ojojojojojo DJL AP a ol ol oo A ND jojojo YD OD DG OD YB GD D O DJ DG B G D O DJ GO oal olojo c oloc ooj clo clo c oloojoo ooj ooj jo ojoo cc olojooj olo ooj olo olo oo o o ooj c oj o o cjoc cicc clox c eEci o olo c eci olelovejoe ecizE eciojeoc izE cicic eoiciololeoelo cojo cdoc ooj olojolojooj oojoo oo olojo do ooj jooj o o oc cc cojooj loojolojololoo c o o o alol olal ol olal olal DG D O MW GD ol nl YD DO DO OD alal ol olala ol nl D wm Dn al olol lololol BW GD DG GD GD DG oal olojo XN O olal OC CO JZ olal DG ojo OD ol ol HD ojo ol YD nl D O YM moja a a a nO D DO DG DG WM DG DG jojojo olojo alol olal ol olal GD BD GD BM OG WM GD OD BM 2 ol OD DO alal ol olala O C BM CK KC DM BW DJ Do DG GD YW ojo OD DG OD oal olojo Mn OO O Z Of oj cl oc oc ol sol DP
23. I x xRCFI NI To determine actual speed required to achieve a different g at the same radius N2 N1 RCF2 RCF2 RCF1 V x N1 RCF1 To determine actual speed required to achieve the same g at a different radius N2 N1 RI R1 R2 vxN1 R2 Thermo Fisher Scientific T1 RT1 1 5 Chapter2 Specifications Dimensions and Weight T1 RT1 Dimensions HxWxD 375 x 400 x 500 mm 380x 575 x 620 mm Packed HxWxD 600 x 580 x 640 mm 630 x 730 x 750 mm Weight uncrated crated 40 kg 52 kg 72 kg 85 kg Electrical Specifications T1 RT1 Operating Voltage 10 230 V 120 V 230 V 120 V 50 60 Hz 60 Hz 50 60 Hz 60Hz Operating current steady 2 8 Arms 4 3 Arms 2 9 Arms 6 8 Arms acceleration 5 5 Arms 9 5 Arms 5 5 Arms 9 5 Arms Power max steady 350 W 600 W acceleration 1000 W 1300 W Heat dissipation 1195 BTU h 2050 BTU h Performance TI RT1 Max power 500 W 800 W Average power 350 W 550 W Refrigeration 235 W T1 RT1 Max speed Swing out 4100 rpm Angle 14600 rpm Max RCF Swing out 3176 xg Angle 23113 xg Max capacity Swing out 4 x 280 ml Angle 6 x 100 ml Microprocessor controlled Display High visibility digital display Memory size 4 programs direct access pre cooling Program protection Recall key lock Speed Range 500 to 14600 rpm Step 10 100 rpm Accuracy 20 rpm Timer Range 30 sec to 99 min hold positio
24. ON Pull the handle on the centrifuge right side towards the front of the unit the lid is automatically unlocked and opens In the case of a mains power outage opening of the lid is prevented by the lid lock safety device It is recommended to wait for the mains power to be switched back on so that this safety device enables the lid to be unlocked refer to 3 6 for manual lid opening Carefully clean the inside of the centrifugation chamber removing any packing residues In fact due to air turbulence solid particles accidentally left in the centrifugation chamber create excessive wear of the chamber itself and of the outer rotating equipment surface A Carefully lower the rotor onto the drive shaft Press down on the rotor until a click is heard Try to lift the rotor When correctly placed it will not move being automatically locked onto the drive 1 2 T1 AT1 3 2 Chapter 3 Installation It is not necessary to orientate the rotor relative to the drive shaft in order to achieve locking The AUTO LOCK rotor mounting system allows rotors to be placed in any orientation Manual Lid Unloc king In the event of mains non availability or power failure opening of the Procedure lid is prevented by the lid locking safety device It is recommended that the user waits for the mains to be switched back on so that this safety device enables the lid to be unlocked Manual lid unlocking must only be done by someone informed of the possib
25. Origin State of the centrifuge User s action 01 Incorrect speed The rotor will brake to a stop Wait for rotor to stop measurement Lid opening is impossible till Wait for the 4 minutes speed is zero and a 4 timeout To erase the message minutes timeout is expired open the lid turn the power switch off and turn the power Switch on 03 Power was At power off the rotor will Press any keyboard button to interrupted during coast to a stop erase this message a run Note if Error 01 has been detected and not cleared as above described the alarm reset is possible only after a safety time of 30 minutes 04 Speed gt 0 RPM at The rotor will coast to a stop Wait for rotor to stop power ON Press any keyboard button to erase this message CLOSE Lid Lid open at start The centrifuge doesn t start if Press any keyboard button to the lid isn t properly closed erase this message Close the lid and press START 06 Lid open in The rotor will brake to a stop Wait for the centrifuge to stop running mode Press any keyboard button to erase this message IMBAL Imbalance The rotor will brake to a stop Wait for the centrifuge to stop Press any keyboard button to erase this message Verify that a balanced load is installed Inspect the rotor and rearrange the tubes or add additional tubes with fluid to balance the rotor 08 Motor Over The rotor will brake to a stop Watt for the centrifuge to stop temperature OR Press any keyboard bu
26. S2 Slojolo E AS AS A AS AS S 0 M U 0 S S S S 0 S S S S S S S S U S 0 SS SS sS S U U 0 S S M 0 Potassium Chlorate 0 S S 0 S U 0 0 0 0 0 0 0 S U MS M U Potassium Chloride S 5 S 0 0 S S Potassium Hydroxide 5 M S 0 S UU 0 0 0 Ss S 0 0 0 0 0 0 0 S U M Potassium Hydroxide conc Potassium Nitrate S 0 0 0 0 0 0 S DOS 0 0 S 0 0 0 0 0 0 0 S S Potassium Permanganate Propane Gas U M 0 0 0 0 0 S 0 0 0 0 0 0 0 V St 0 0 0 0 0 0 0 0 Sd U M Sd S 0 0 0 0 0 0 Propionic Acid S 0 0 0 0 0 0 S S 0 0 0 0 0 S S 0 0 0 0 0 St S 0 0 0 0 0 0 0 S Propyl Alcohol S 0 0 0 0 0 0 0 S Sd s 0 0 0 0 0 0 0 0 8 St Propylene Glycol S Propylene Oxide Pyridine U 0 0 0 0 0 0 0M US MO 0 0 0 0 0 0 0 0 0 0 0 0 0 8 Sd S U 0 0 0 0 0 S Resorcinol Sat d Sol Thermo Fisher Scientific T1 RT1 6 8 Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO M 0 0 0 0 0 0 0 S Chemical S 0 S 0 0 0 0 0 0 S Rubidium Bromide Saccharose S 0 0 0 0 0 S 0 0 0 0 0 S S S U 0 0 0 0 0 0 0 5 0 0 0 0 0 0 0 0 S Sd S S S 0 0 0 0 0 0 0 S Salycylic Acid Sat Serum 0 S S 0 S S S S 0 0 0 0 0 S 0 0 0 0 0 0 0 0 S Sd S U 0 0 0 0 0 0 0 S Silver Acetate Silver Nitra
27. Sorvall T1 RT1 User s Manual Analyze Detect Measure Control SCIENTIFIC T1 RT1 Sorvall T1 RT1 Users Manual Carefully read this manual before operating your instrument Information contained in this document is the property of Thermo Fisher Scientific it may not be duplicated or distribuited without the owner s authorization The validity of the guarantee is subject to the observation of the instructions and precautions described in this document Preface MANUAL NUMBER REV ECR ECN DATE DESCRIPTION BY a 11290097 02 07 Initial release Thermo Fisher Scientific T1 RT1 i T1 RT1 Packing List LI 75002381 Centrifuge T1 230V 50 60 Hz 75002382 Centrifuge T1 120V 60 Hz 75002392 Centrifuge T1 100V 50 60 Hz L 75002383 Centrifuge RTI 230V 50 60 Hz 75002384 Centrifuge RT1 120V 60 Hz LI 75002393 Centrifuge RT1 100V 50 Hz LI 75002395 Centrifuge RT1 100V 60 Hz Item number Quantity Check Centrifuge 1 LI 11290097 User manual LI Manual lid opening instructions LI 64227001 Mains cord LI Toolbox 11202979 Rotor removal tool LI 25535010 Emergency unlocking tool 2 LI 26387032 Spare fuses T1 only 1 LI For missing parts call your nearest Thermo representative Thermo Fisher Scientific Great Britain Deutschland Thermo Fisher Scientific WEEE Compliance WEEE Com
28. ature of contamination Decontamination certified by le EE STILO cora EI Signature sirsa When an instrument or parts of it requires servicing by Thermo SAT or CSAT personnel the following procedure must be accomplished to ensure personnel safety e Clean the instrument and proceed to its decontamination from any kind of dangerous products e Compile this Decontamination Certificate with all the information required T1 AT1 1 1 Certificate of Decontamination and Cleaning lii 2 TI RT1 Attach this Certificate to the instrument or part before sending it to Thermo Service or other authorized technical service SAT and CSAT personnel will not accept to work on instruments deprived of this Decontamination Certificate If an instrument is received at our Service facilities and in our opinion is a radioactive or biological hazard the item will be refused and resent to the Customer Disposition costs will be borne by the sender Instructions for decontamination and cleaning are explained in the User Manual Additional certificates are available from your local technical or Customer Service representative In the event these certificates are not available a written statement certifying that the instrument or part has been properly decontaminated and outlining the procedures used will be acceptable For your convenience use a copy of this page Thermo Fisher Scientific P N 11290097 a
29. ax Tube 9 Radius Max Speed Max Angle mm mm rpm RCF xg deg 11175743 Drum rotor 6 racks 72 14600 17156 90 11174561 1 rack 10x 1 5 2 mL 11 11174573 1 rack 20 x 500 800 uL 8 11174574 1 rack 20 x 700 uL 6 11174563 1 rack 21 x 600 uL 6 11174562 1 rack 32 x 250 400 uL 6 Total rotor load is 60 120 120 126 and 192 microtubes 3 7 T1 RT1 Thermo Fisher Scientific Chapter 4 Instructions for Use Controls and Indicators All the controls are located on the front panel The front panel is an intuitive interface no previous operational knowledge is necessary every parameter can be set by pressing the cursor key continuously from the minimum to the maximum and viceversa All parameters can be accessed and changed both during 11 14 la 1 12 10 operation and while the machine is at rest 1 la 2 2a 15 18 16 20 8a 9a 17 Buttons LED Lights 1 4 Program Keys la Program 1 Indicator 5 Pre Cooling 2a Program 2 Indicator 6 Speed RCF Toggle 3a Program 3 Indicator 7 Time Radius Toggle 4a Program 4 Indicator 8 Acceleration rate set ba Program Precooling Indicator 9 Deceleration rate set 8a Acceleration Indicator 10 Stop Run 9a Deceleration Indicator 11 Pulse Run 14 Lid Opening allowed Indicator 12 Start Run 18 Speed Indicator 13 Temperature set R only 19 RCF Indicator 20 Time Indicator Display Screens 21 Radius Input Indicator 15 Display speed RCF 16 Display time raduis 17 Display accel decel rates temp R mod T
30. centrifuge like the T1 exhausts into the room air which has passed through its bowl Thus the risk of the spread of aerosols is significant in a ventilated centrifuge In the case of samples presenting a biological or chemical risk the operator must take suitable precautions to prevent or reduce this risk Thermo Fisher Scientific Duration of aerosol presence Mechanical Risks Corrosion Information Thermo Fisher Scientific Chapter 5 Hazard Precautions and Limitations of Use Thermo recommends the use of its sealed buckets which are certified for bio containment through worldwide recognized testing procedures performed in recognized external laboratories A Aerosols are produced naturally when using uncapped tubes or bottles They are also produced when a container deforms or breaks provoking a small or large sample leak We draw your attention to the use of tubes in angle rotors Even if the liquid level does not touch the cap when in a static state it could do so during centrifugation and leak You should therefore follow the instructions of the tube supplier concerning the fill level and sealing of the tube Once a biological risk is known or suspected samples should be placed in a sealed container Should such containers not be available sealed accessories should be used such as angle rotors with sealed lids d Y When aerosols are created in a centrifuge bowl they persist in the air for a period of 3 5 minut
31. ci WEEE E marcato col seguente simbolo Thermo Fisher Scientific ha stipulato contratti con una o diverse societ di riciclaggio smaltimento in ognuno degli Stati Membri Europei Questo prodotto verr smaltito o riciclato tramite queste medesime Ulteriori informazioni sulla conformit di Thermo Fisher Scientific con queste Direttive l elenco delle ditte di riciclaggio nel Vostro paese e informazioni sui prodotti Thermo che possono essere utili alla rilevazione di sostanze soggette alla Direttiva RoHS sono disponibili sul sito www thermo com WEEERoHS WEEE Conformidad Este producto requiere cumplir con la Directiva 2002 96EC WEEE residuos de quipos electricos y electronicos Este producto esta marcado con el simbolo WEEE como se describe En cada Estado Miembro de la Union Europea EU Thermo Fisher Scientific ha contratado con diversas companias para disponer y o re ciclar los residuos de equipos electricos y electronicos Existe informa tion adictional acerca de la conformidad de Thermo Fisher Scientific con esta Directiva incluyendo los nombres de las diversas companias TI RTT iii WEEE Compliance iv T1 RT1 France autorizadas para disponer recyclar nuestros productos Adicionalmente suministramos informacion acerca de los productos de Thermo Fisher Scientific gue puedes asistir en la deteccion de substancian mensionades en la directiva RoHS explicado en www thermo com WEEERoHS Conformit WEEE Ce produit doit tre c
32. d to verify proper appearance of screws lid hooks latches and for evidence of corrosion In case of problems contact your Thermo Service representative because any deviation from the above mentioned advice may have serious consequences for the safety of the appliance and of the operators A Normal use of the centrifuge could require the manipulation of biohazards Users and service personnel must have specific training for each substance they use according to the Laboratory Biosafety Manual from the World Health Organization A The contents of each rotor pocket including sample tube cap and adapter where used must be of the same weight as the one diametrically opposite Imbalance of the rotor may cause major damage to the rotor and centrifuge Do not attempt to introduce liquids into rotor pockets or into tubes already in the pockets A Distribute the load correctly Loads should be inserted two by two samples of equal weight at 180 degrees from the rotation axis and at equal distance radius from the center of rotation see pictures When using fixed angle rotor weight deviation between the tubes must not exceed 5 grams Thermo Fisher Scientific Chapter 4 Instructions for Use Correct When using a swing out rotor the samples are placed in plastic inserts The load must be statically and dynamically balanced e Static balancing consists of balancing the weight of the 2 inserts which are in oppos
33. e e 212181218 ese e sjejejejeje e el te E O ajojojojojo lo U M 0 0 0 0 0 U S 0 0 0 0 0 0 0 0 V St S Cinnamon Essence Citric Acid 1096 Copper Nitrate S S S 0 S 0 0 0 0 0 0 S S S S S S MS S 0M S U 0 0 0 0 0 0 0 6 6 5 T1 RT1 Thermo Fisher Scientific Chapter 6 Service and Maintenance AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Material PPCO U 0 0 0 0 0 0 0 8 S 0 0 0 0 Chemical S 0 0 0 0 0 0 0 St Copper Sulphate Croesol S 0 0 SMS 0 U S U S Sd Mt Mt S SM SS S 0 00000 U S 0 0 0 0 0 0 0 Mt S 0 Cyclohexane 0 S 0 S005 U 0000 Cyclohexanol U 0000 U 0 0 0 0 U U U U U U U U 0 0 0 0 0 0 0 0 Mt 0 0 0 0 0 0 0 0 0 0 Cyclohexanone Cyclopentane Decane 0 0 0 Mt 0 0 O M Mt Mt Mi Sd 0 0 0 0 0 S S05 0 0 S 0 0 S S S M0000000 S S 0 Dextran Sulphate Diacetone 0 S 0 0 0 0 0 S 0 S 0 U 0 S 0 0 0 0 0 0 S 0 0 0 0 0 0 0 S Diacetone Alcohol o lo vo o lo o vo Oo JoJo jojojo o O no O O o O O vo O c o O wo JOJO mo lo 5 O 5 S jov o o c O O vw O O vb O c o o o o o o o o O o oo o o 2 o 2 5 p S JO jo gt jo jo jo S S gt lol jm ln 5 5 lulu leo 5 LEmxDDDm5Inm e eoiml ocowio z Ss
34. es after the rotor stops moving Indeed the action of opening the lid itself causes the dispersion of aerosols into the environment When an elevated risk of aerosols or of breakage is perceived the accessories should be handled using gloves and opened in an environment ensuring the protection of the operator safety cabinet glove chamber wearing a mask even if they are apparently sealed A Thermo rotors made of aluminium alloy are designed to operate at their rated RCF for many years With careful use they will resist corrosion lowing the possibility of excessive imbalance disruption and subsequent damage to the instrument The primary conditions for the initiation of corrosion exist in every laboratory during daily use of the centrifuge For this reason it is essential that due care and attention be paid to inspection and cleaning T1 RT1 5 3 Chapter 5 Hazard Precautions and Limitations of Use 5 4 T1 RT1 Chemical Corrosion Stress Corrosion This corrosion is characterized by chemical reactions due to the existence of any electrolyte liguid on the surface of the rotating eguipment If these substances are allowed to remain on the surface the corrosion will almost certainly occurs This produces first a discoloration of the anodization the pitting of the metal Acidic and alkaline solutions sustaining their pH level will create problems of corrosion in aluminium eguipment Chlorides present in salts or even in s
35. g mit folgenden europ ischen Richtlinien befindet Nostros Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que el instrumento bajo descrito est fabricado seg n las especificaciones establecidas por las siguientes normas europeas N s Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declaramos que o instrumento aqui apresentado est de acordo com as normas europ ias abaixo listadas Noi Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France dichiariamo che lo strumento sotto descritto conforme alle seguenti Direttive europee e Machinery 98 37 CEE Low voltage 72 23 CEE 93 68 CEE e EMC 89 336 CEE 92 31 CEE 93 68 CEE e Safety standard EN 61010 1 EN 61010 2 020 TI AT1 v Guarantee Terms Thermo guarantees that this unit is free from defects in materials and workmanship when it leaves the factory and will replace or repair the unit if it proves defective in normal use or during service for a period of ONE YEAR from the delivery This guarantee is invalid if the unit is incorrectly used poorly serviced or neglected mis used or accidentally damaged There is no explicit guarantee other than as stated above vi T1 RT1 Thermo Fisher Scientific For further information assistance or service United States of America Tel 1 888 213 17 90 Tel 1 866 984 37 66
36. gal force provided by the centrifuge can also be used to drive solvents and low molecular weight solutes through the membrane of a filtering device Retained macrosolutes will therefore be found above the membrane T1 RT1 1 1 Chapter 1 Use and Function 1 2 Refrigeration and Heating Systems T1 RT1 Condensation drain on refrigerated units Relative Centrifugal Force RCF generated by a rotor is directly proportional to its sedimentation useful radius and to the value of its speed squared A control system permits the user through an extremely intuitive control panel to set and control the speed the g force the temperature on the thermostated and refrigerated versions and the run time as well as to view different messages and warnings The TI is a ventilated unit A permanent air circulation reduces the heat level in the bowl However the temperature in the bowl can reach 10 C above the ambient temperature after hours of continuous operation The RT1 is equipped with a powerful refrigeration system that allows samples to be processed at low temperature The refrigerant R134a is free of CFC Chloro Fluoro Carbons in accordance with the Montreal Protocol directions for preservation of the ozone layer in the atmosphere The actual temperature in the bowl is continuously displayed on the front panel of the centrifuge Under certain conditions high speed long duration runs the sample temperature can be higher than t
37. he bowl temperature on the display In this case the user may compensate manually after empirical determination of the temperature differential The lid should remain closed when the refrigeration system is in use to avoid condensation ia REFER TO INSTRUCTION MANUAL The centrifuge has a rubber hose that allows condensation to be drained from the bowl The drain hose exits at the back of the machine Near the drain hose you can find the following label To remove condensation please follow this procedure e Switch off the centrifuge and disconnect the power e Puta small basin under the hose Thermo Fisher Scientific Drive System Safety Interlock System Imbalance Detection Thermo Fisher Scientific System Chapter 1 Use and Function e Unplug the hose e Drain the centrifuge bowl e Insert the plug back into the hose Attention Do not remove the plug from the drain hose during centrifugation A A three phase brushless motor drives the rotating equipment The rotor is contained in a sealed armor plated centrifugation chamber The brushless motor requires no maintenance A control system ensures the correct drive speed which is continuously monitored The TI and RTI centrifuges are equipped with an interlock system that assures two basic safety features e The run cannot be started if the lid is not correctly closed e The centrifuge lid cannot be opened if the rotor is running Current safety
38. hermo Fisher Scientific T1 RT1 4 1 Chapter 4 Instructions for Use Display Screens Screen Description Display 15 This screen displays the set speed rpm or RCF x g for the given program when the centrifuge is at rest depending on which parameter is selected During a run this screen displays actual speed or RCF Display 16 This screen displays either the set run time or the centrifugal radius when the centrifuge is at rest depending on which parameter is selected During a run this display shows only time remaining timed runs or time elapsed during deceleration hold runs and pulse runs Display 17 on the TI This screen displays either the acceleration or the deceleration profile selected for the given program The value of the acceleration profile is displayed either during acceleration or when the acceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed Display 17 on the R units This screen displays either the acceleration or the deceleration profile selected for the given program The value of the acceleration profile is displayed either during acceleration or when the acceleration set button 8 is pressed The value of the deceleration profile is displayed either during deceleration or when the deceleration set button 9 is pressed In addition it shows the temperature in the bowl of the ce
39. hould be replaced when deformed Any part which has been subjected to temperatures above 130 C must be discarded Hypochlorite bleach used at 0 196 concentration with 10 minutes immersion is effective against bacteria spores and viruses but as an oxidizing agent is corrosive to metal alloys and must be thoroughly T1 AT1 6 1 Chapter 6 Service and Maintenance Radioactive Decontamination Power Supply Circuit 6 2 Breaker Rotor Removal T1 RT1 rinsed off of metal parts and the parts dried It should never be used if there is surface damage to the rotor Formalin 37 formaldehyde in water in contact for 10 minutes has a similar effect to chlorine bleaches Rotors should be thoroughly rinsed under running water for 5 minutes to remove all traces of formalin then dried completely Formaldehyde is toxic A Gluteraldehyde 296 sold under many brand names such as Cidex and Glutarex requires total immersion for 10 minutes to ensure sterility Thorough rinsing and drying is essential to protect users Gluteraldehyde builds up to a toxic level in the fatty tissues of the body Phenols are very corrosive and should never be used A We recommend that all radioactive contamination be referred to your Radioactivity Safety Officer Rotors may be decontaminated by a mixture of equal volumes of Distilled water e SDS diluted to 1090 Ethanol diluted to 7090 The rotor should then be rinsed with ethanol
40. indicator LED is not illuminated the lid cannot be opened The rotor is still in motion This feature is in accordance with international safety standards T1 RT1 4 3 Chapter 4 Instructions for Use Description of Certain Events with Respect to the Control System Start and acceleration phase 4 4 T1 RT1 Set error During the run Stop and braking deceleration phase End of run When the start button is pressed the centrifuge will begin to accelerate the rotor At this point the run timer will start and the middle display will begin to show remaining run time The upper display will show either actual speed or actual maximum RCF values whichever is currently selected The lower display shows the given acceleration profile and the LED corresponding to the acceleration button is illuminated During acceleration the centrifuge automatically detects the rotor type and therefore its maximum allowed speed If the operator sets a speed exceeding this the centrifuge stops acceleration when the maximum allowed speed is reached ensuring the operators safety The display will blink and an audible alarm will invite the operator to correct the speed setting to a proper value During the run the upper display will show either actual speed or actual maximum RCF values whichever is currently selected The middle display will show either remaining time timed run or elapsed time hold and pulse runs During a run
41. ite locations in the rotor 8 to 10 grams e Dynamic balancing consists of placing the tubes in each insert symmetrically according to the axis of the trunnions Thermo Fisher Scientific Dynamic Balancing Correct ZI Incorrect If less than the maximum complement of samples is loaded the tubes must be placed in opposite pockets An odd number of tubes requires an additional blank water filled tube of identical total weight to balance Significant vibrations can damage the accessories the unit and the sam ples Most vibration is caused by incorrect loading of the tubes For this T1 RT1 4 7 Chapter 4 Instructions for Use reason the centrifuge is equipped with an imbalance detector In case of excessive imbalance the load imbalance indicator LED is illuminated and the brake is applied immediately The rotor will be decelerated to rest in few seconds As soon as the motor stops open the centrifuge and redistribute the samples to produce an equal weight on diametrically opposite sides Also remember that the pins must be softly lubricated Inadaquate lubrication of the pins may cause incorrect swinging of the buckets resulting in imbalance phenomen See chapter 6 6 for details If the message persists despite your efforts to balance the load call your Thermo Service representative 4 8 T1 RT1 Thermo Fisher Scientific IEC 1010 2 020 Thermo Fisher Scientific Cautions Chapter 5 Hazards Precautions and
42. kin contact with the rotating eguipment are among the most aggressive and harmful substances commonly found in the laboratory The chemical products which are the origin of this corrosion do not necessarily originate from broken tubes for example they could come from e chemical vapors present in the laboratory which are dissolved in the residual humidity in condensed water refrigerated centrifuges present at the base of the rotor pockets corrosive liquids originating from overfilled uncapped tubes the liquid overflowing during centrifugation e Inserts adapters racks bottles whose exterior has been soiled by a chemical product or poorly rinsed after decontamination with bleach for example Note If the products are very corrosive simply rinsing is insufficient Residual traces dissolve little by little with the humidity present in the bottom of the rotor pocket A Beware of the presence of solid particles beneath tubes inserts racks or adapters These particles are crushed by the centrifugal force and penetrate the protective anodized layer of buckets and rotors thus creating easy pathways for corrosion A This term relates to the phenomenon of accelerated corrosion due to the effect of centrifugation when a corrosive chemical is in contact with the alloy From the time when the aluminium alloy has been attacked by chemicals stress corrosion begins to appear As it is on a microscopic scale it is even more dangerous
43. le it is essential that rotating equipment are scrutinized regularly paying particular attention to susceptible parts such as the bottom of pockets the outer edges and the base of the rotating equipment A Thermo centrifuges are likely to be used in medical research and Contamination Hazard laboratory where hazardous substances are frequently present The user has the responsibility for carrying out appropriate decontamination and elimination of the handled materials Users should be aware on the internationally recognized Laboratory Biosafety Manual which gives information on decontaminants their use dilutions properties and potential applications A If decontamination procedures require the use of warming i e autoclave the rotating equipment should always be completely disassembled before being subjected to heat and after external chemical cleaning Seals Tubes and plastic components should be decontaminated with the method most suitable for them which might not be the same as for rotating equipment Note that the black color on the surface of the rotating equipment will be gradually washed out if the rotor is regularly autoclaved or bleached This does not necessarily denote degradation on the anodization A Thermo Fisher Scientific T1 RT1 5 5 Chapter 5 Hazard Precautions and Limitations of Use Any part which has been subjected to temperatures above 130 C must be discered A manufacturer to verify that the propo
44. le accessories and the material supplied for ordinary operations and maintenance e Check the contents of the package using the packing list provided above e Keep the packaging until the centrifuge has been tested and found fully functional Thermo Fisher Scientific Positioning Mains Supply Lid Opening and Rotor Checking To install the rotor Thermo Fisher Scientific Chapter 3 Installation The machine must be installed in a dust and corrosion free room Leave a 30 cm 12 in space free on each side of and behind the machine for safety reasons proper ventilation and maximum cooling performances A Place the centrifuge on a bench top which must be rigid horizontal and sufficiently strong to support the centrifuge s weight and small vibrations Check mains and frequency they must correspond to the values shown on the instrument identification label For your safety check that mains wiring is effectively grounded Thermo declines all responsibility for any damages due to non grounding of the machine A Remember that in order to respect the electrical safety standards related to protection against indirect contact the supply of power to the instrument must be via a power socket fitted with a protection device ensuring automatic cut off in the case of an insulation fault A supply fitted with a circuit breaker of the correct rating complies with this requirement Ensure that the centrifuge has been switched
45. le danger and of the necessary precautions Rotating parts are a risk as they could come in contact with the user or be ejected There is particularly high risk of injury if e The user attempts to manually stop the rotor Any object falls inside the centrifugation chamber while the rotor is running A Manual lid unlocking may be necessary under a very limited number of conditions such as the urgent recovery of critical samples that could be damaged if left in the centrifuge rotor In this case the lid can be opened by using the special tool supplied with the centrifuge I Always set the power switch to the OFF position before performing this manual procedure even in the case of a mains power outage A Should the emergency occur due to power failure during centrifugation wait at least 10 minutes for the end of the rotor rotation In spite of the absence of noise the rotor could still be rotating when you need to open the lid manually Upon opening lift the lid by hand and observe the rotor be careful in this operation if it is still rotating close the lid and wait 3 3 T1 RT1 Thermo Fisher Scientific Chapter 3 Installation Figure 1 Insert the unlocking tool horizontally into the hole on the right hand side of the instrument Figure 2 While pressing up with your finger to press down with the unlocking tool pull the lid lever toward you the lid opens upwards The protection sticker must be replaced after it has
46. ly A T1 AT1 6 3 Chapter 6 Service and Maintenance Chemical Compatibility Table for Rotors Tubes and Accessories S Satisfactory AL Aluminum BN BunaN D Discoloration but OK CAB Cellulose acetate butyrate CN Cellulose Nitrate P Pure chemical OK DL Delrin KY Kynar M Moderate resistance NO Noryl NY Nylon U Unsatisfactory PA Polyallomer PPCO PC Polycarbonate X Explosion risk PE Polyethylene PP Polypropylene O No information PPCO Polypropylene Copolymer PS Polysulfone d Less resistance if T gt 50 C SS Stainless steel TF Teflon t Unsatisfactory if T gt 50 C TZ Iefzel T Titanium VA VitonA Material AL BN CAB CN DL KY NO NY PA PC PE PP PS SS TF TZ TI VA Chemical PPCO Acetaldehyde S U U U O 0 00M UMM US SMS U Acetamide 00 0 0 0 0 0 0 SUS SS 0 0 0 0 0 Acetic Acid 5 SMS SMS SS S SS DS SS S SM Acetic Acid 20 S 0 0 0 0 0 0 0 SMS SS 0 0 0 0 0 Acetic Acid 60 S U U U U SS MS UM DS SS S S U Acetic Acid 80 S 0 0 0 0 0 0 0 SU St S M 0 0 0 0 0 Acetic Acid Glacial S U U U U Ss 0 0 SUMDMS SS S U Acetic Anhydride S 0 0 0 0 0 0 0 Sd UU Sd U OO 0 0 0 Acetone s U U UM M0 US US MU S S MS U Acetonitrile 0 0 0 00 0 0 OM U SM U 0000 0 Acetylene S 0 0 0 0 0 0 0 S 0 SS U 0 0 0 0 0 Adipic Acid 0 0 0 0 0 00 0 S SSS S 0 0 0 0 0 Alanine S 0 0 0 0 0 0 0 SS U SUV 0 0 0 0 0 Allyl Alcohol 00
47. m position The corresponding LED 1a 4a will be illuminated While the LED is illu minated enter the desired parameter values These values are automatically saved in memory To lock a program To lock a program press and hold the corresponding button for several seconds until the correspon ding LED begins to flash Release the button and the program is locked Parameters of the program cannot be changed To unlock a program To unlock a program when a program is locked the associated LED flashes press and hold the corresponding button for several seconds until the associated LED stops flashing Release the but ton and the program is unlocked Precooling program Select the Precooling program pressing button 5 the LED 5a will light on Only temperature setting is required Speed and Time are defined by the software according to the rotor in use Press button 12 to start the precooling program When the rotor reach the selected temperature the 5a LED will blink in combination with acoustic signal Press button 10 to exit NOTE The precooling program can not be locked Use empty rotor without lid Control Panel Indicators Indicator E Thermo Fisher Scientific Description When the lid interlock indicator LED 14 is illuminated the lid interlock is not active The lid can be opened The indicator will only be illuminated when the rotor is at rest and it is safe to open the lid When the lid interlock
48. n Step 30 sec to 1 min 2 1 T1 RT1 Thermo Fisher Scientific Chapter 2 Specifications T1 RT1 Acceleration rates 5 Braking rates 5 Temperature RT1 Range 9 C to 40 C Step 186 Accuracy 1 5 Typical performance 4 C at 4 000 rpm 4 x 280 ml swing out 1 C at 14 000 rpm 20 x 1 5 ml angle Maximum density 1 2 g cm3 Maximum energy 14 400 J Thermo Fisher Scientific T1 RT1 2 2 3 1 T1 RT1 Environmental Conditions Unpacking Chapter3 Installation General conditions accepted for centrifuge transport and storage are Ambient temperature 20 C to 50 C e Relative humidity up to 90 General conditions accepted for operating the centrifuge safely are e Indoor use e Temperature 5 C to 40 C e Maximum relative humidity of 85 e Maximum altitude 2000 m e Installation category II e Polution degree 2 Due to the weight of the machine all lifting and transporting must be carried out using proper handling equipment i e fork lift trolley that complies with current regulations and by people having undergone the necessary training Thermo strongly recommends that all operators comply with the local laws and regulations on safety and health in the workplace The machine must be supported from underneath If it has to be transported without its pallet for example on a staircase professional handling assistance is required A e Unpack the centrifuge carefully removing any possib
49. ntrifuge When the centrifuge is at rest the set bowl temperature is displayed C During a run the actual bowl temperature is displayed C Control Panel Functions Function Start cycle Keystroke sequence and description Press the start button 12 Stop cycle immediate Press the stop button 10 Pulse Press the pulse button 11 and hold The centrifuge will accelerate the rotor to the speed setting of the active program When the button is released the rotor will be decelerated to rest Set speed When the LED next to RPM 18 is illuminated the upper display shows speed values To increase decrease the speed values press on the corresponding up down arrows Set RCF When the LED next to G 19 is illuminated the upper display shows RCF values To increase decrease the RCF values press on the corresponding up down arrows When using RCF as a control display value make sure the correct centrifugal radius is entered into the control system see below If an incorrect radius value is in the control system the RCF value displayed will not be accurate Set run time When the LED next to TIME 20 is illuminated the middle display shows time values To increase decrease the run time values press on the corresponding up down arrows Set centrifugal radius 4 2 T1 RT1 When the LED next to RADIUS 21 is illuminated the middle display shows the value of the cen trifugal
50. onforme la directive euro p enne 2002 96EC des D chets d Equipements Electriques et Electroniques DEEE Il est marqu par le symbole suivant Thermo Fisher Scientific s est associ avec une ou plusieurs compagnies de recy clage dans chaque tat membre de l union europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Fisher Scientific ces directives les recy cleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la d tection des substances sujettes la directive RoHS sont disponibles sur www thermo com WEEERoHS Thermo Fisher Scientific Declaration of compliance Declaration de conformite Konformit tserkl rung Declaraci n de conformidad Declaracao de conformidade Dichiarazione di conformit Thermo Fisher Scientific Declaration of compliance We Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France declare that the instrument defined here below complies with the following European directives Nous Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France d clarons que le mat riel d sign ci apres est conforme aux directives europ ennes suivantes Wir Thermo Electron Industries S A S Z I d Aze Bellitourne 53200 CHATEAU GONTIER France erkl ren dass das hier beschriebene Ger t sich in bereinstimmun
51. pliance This products is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State and this products should be dis posed of or recycling through them Further information on Thermo Fisher Scientifics compliance with these Directives the recyclers in your country and information on Thermo Fisher Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEERoHS WEEE Konformittat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96ECerf llen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen getroffen mit Verwertungs Entsorgungsanlagen in allen EU Mitgliederstaaten und dieses Produkt muss durch diese Firmen widerverwetet oder entsorgt werden Mehr Informationen ber die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific die Verwerter und Hinweise die Ihnen n tzlich sein k nnen die Thermo Fisher Scientific Produkte zu identizfizieren die unter diese RoHS Anweisung fallen finden Sie unter www thermo com WEEERoHS Conformita WEEE Questo prodotto deve rispondere alla direttiva dell Unione Europea 2002 96EC in merito ai Rifiuti degli Apparecchi Elettrici ed Elettroni
52. ric Acid 5096 Hydrochloric Acid conc Hydrofluoric Acid 1096 Hydrofluoric Acid 5096 Hydrogen Peroxide 396 Hydrogen Peroxide 10096 lodine Crystals Isobutyl Alcohol Isopropyl Alcohol Isopropylbenzene Kerosene Lactic Acid 2096 Lactic Acid 10096 Lead Acetate aq Lemon Essence Lime conc Magnesium Chloride Magnesium Hydroxide Magnesium Nitrate Magnesium Sulphate Manganese Salts Mercury Methanol 10096 Methyl Acetate Methyl Alcohol 10096 Methyl Butyl Ketone Methyl Ethyl Ketone Methyl Isobutyl Ketone Methyl Isopropyl Ketone Methylene Chloride Mineral Oil Nickel Chloride Nickel Salts gt U e SS TF TZ TI VA co EE a co a o co e co e co e o co c Ol al al a no wm oc C wm wm Zl to C C C C C co co Raid oo So of a nm a zZ Lp a cj a ul a Ql a Ql Yl a wm ao an co co ow FS an ni a aj nl zZ Lj a cj a a a Si ro Cl ZI old nm wm nl oOo ol nl wml Cc Oo co wm Go co Gol Oo vn nm wm oO Zn Zlol Zl l_ cl cl of of mH Of cl O WH mM oj C co Zl alal oaj olal Zi ajl aja g Loja dolo os g ol oa El aj aj al oal olaj ajaj El z a aj a aj a Z Lo man Cc cicicio jco c co co O oo QOoO co co O QO O co XM OO O C Of CO C DO MD DG BM GD D
53. sed method does not damage the appliance A D For any other unscheduled cleaning the customer must contact the Thermo makes no claims as to the effectiveness of proprietary brands of decontaminating solutions 5 6 T1 RT1 Thermo Fisher Scientific Periodic Cleaning Daily Weekly Disinfection Thermo Fisher Scientific Chapter 6 Service and Maintenance All cleaning operations should be performed with the centrifuge disconnected from the power outlet A No daily cleaning is required except in the case of accidental tube breakage or when there is a large amount of spillage in the bowl Clean the bowl and the accessories with a cotton wool pad dipped in 70 alcohol Never use metallic brushes to clean accessories e After cleaning the accessories rinse them in running water preferably distilled water e Dry the rotor with a soft absorbent non woven cloth or tissue Drying may be finished off with a warm air jet e g a hair dryer e Make sure that the pockets are well dried e Ideally spray with Anti Corrosion Spray Cat N 11175399 Store unused buckets upside down on a non metallic grid to allow free passage of air Alcohol 7096 ethanol or isopropanol applied for 10 minutes is ideal for bacteria and viruses Autoclave for 20 minutes at 120 C to destroy micro organisms Polypropylene components are autoclaveable Rotor lids must be disassembled from rotor bodies O rings autoclaved separately s
54. te 0 S S 0 S S S S 0 0 0 0 0 S 0 0 00 0 0 0 S Sd S S M 0 Sodium Acetate S SS 0 S S 0 0 S 0 SS S S S Sodium Bisulphate Sodium Borate S Sd S 0 0 0 0 0 0 S 0 S 0 0 0 0 0 0 S S DOS S S M 0 0 0 0 0 0 0 U 0 0 0 0 0 0 0 S Sodium Bromide S M S S S M S S S S S S S S S S S S S S S S S S S S S S S S S S S S S S 0 0 S U MS S U Sodium Carbonate 2 Sodium Chloride 10 S S S S S 0 0 0 0 S 0 S S 0 S S Sodium Chloride Sat d S S S S S S S S S S US MO S S U U S 0 S 0 Sodium Hydroxide 5190 U U U U U U M M MM S S U Sodium Hydroxide 1096 U 0 0 O M S Sodium Hydroxide conc S SM S S S 0 S 0 0 0 0 0 0 0 Sd S 0 0 0 0 0 0 O Sd Sd 0 0 0 0 0 0 U SS 0 Ss S D S Sodium Hypochlorite 596 Sodium lodide M 0 0 0 0 0 0 OS S 0 0 0 0 0 0 0 S Sodium Nitrate S 0 0 0 0 0 0 0 Sad Sodium Sulfate M S 0 0 850 S S S S 0 0 0 0 0 0 0 S Sd S Sodium Sulphide Stearic Acid S 0 0 0 0 0 S S S S S SMS S U S SUSS U 0 0 0 0 0 0 0 Sd Sd S Sd 5 00 0 0 0 U Sulphuric Acid 10 Sulphuric Acid 20 Sulphuric Acid 50 Sulphuric Acid conc M S S S S S 0 0 0 0 0 U S U S S U SO U S 0 0 00 0 0 085 Sd S S M UM D U D U S
55. than a macroscopic since it is invisible During centrifugation chemicals responsible for corrosion are also submitted to the very high g force which pushes them against the alloy This close contact facilitates the chemical reaction which occurs much faster than in a static situation Moreover centrifugal force is Thermo Fisher Scientific Chapter 5 Hazard Precautions and Limitations of Use very directional thus corrosion under stress creates with a very small amount of corrosive product straight microscopic fissures Each centrifugation run makes the chemical migrate further and further Fissures or cracks although it is microscopic are a cut in the metal breaking the cohesion of the material As one weak link in a chain allows the chain to break so the micro fissures break the chain of resistance of the accessory to centrifugal force As accessories are designed with high levels of safety rupture does not occur as soon as the first micro fissures are produced Depending upon the location of the fissure disruption may occur before it reaches the external surface of the accessory The fissure creates a weakness which makes the accessory less and less resistant to mechanical fatigue The corrosion by a small amount of corrosive product does not disrupt the accessory but makes it mechanically weaker and weaker until disruption due to both centrifugal effort and number of cycles Because stress corrosion is largely invisib
56. tton to Lid lock error erase this message Wait for motor cooling down 09 Chamber Over The rotor will brake to a stop Wait for the centrifuge to stop Temperature Refrigeration Failure Press any keyboard button to The unit displays Refrigerated only erase this message this code if the Verify the operating condition measured temperature exceeds 50 C at any time during the run 11 Electronic failure All controls are disabled To erase the message turn the power switch off and turn the power switch on Note All error indications can be cleared by turn the power switch off and turn the power switch on If error code reappears and the cause of the problem cant be removed contact your local Thermo Service representative A T1 RT1 4 5 Chapter 4 Instructions for Use Preparing the First Run of the Day n AD Sample Loading 4 6 T1 RT1 Before installation the rotor should be thoroughly inspected for evidence of corrosion or other damage and for cleanliness Chemical and stress corrosion of metallic parts will eventually lead to disruption of the rotor with potential severe damage to the centrifuge Particles stuck inside the pockets can cause breakage of tubes and lead to major imbalance and or loss of sample and contamination The central hole of the rotor and the drive spindle should also be clean and undamaged These parts should be wiped clean before each use The centrifuge also should be observe
57. ua aku n E 1 3 eet ege 0168 pic 1 4 Specifications vun daa a ka AAA ka n 2 1 Dimensions and Mecht suu scssksssscu id 2 1 Electrical Specifications os SRE 2 1 Performance PTT TR my 2 1 Ec 3 1 Environmental Condo aan 3 1 Unpacking sss eoo 3 1 E TTT NEETER 32 Pu Seto assault 3 2 Lid Opening and Rotor Checking pai 3 2 Manual Lid Unlocking Ce IE sss nnana 3 3 Performance and Accessories misil dd 3 5 Instructions for USE iii 4 1 Controls and to cessi E sk 4 1 Display Sereno asas 4 2 Control Panel Pantone add 4 2 Control Panel Indicators narrada 4 3 Description of Certain Events with saua sesia gg slas ges ete 4 4 Preparing the First Run of the Dar an 4 6 Sample Loading cae ca ee a kae s g uti 4 6 Hazards Precautions and Limitations of Use 5 1 WS I c 5 1 Ta PH capes M 5 1 Speed Control ttc 5 2 Operational Liens a a dtt ri es urea pec 5 2 Aerosol gl C okan e ARTE 5 2 Mechanical RISKS PT V S 5 3 Thermo Fisher Scientific Thermo Fisher Scientific Chapter 6 Contents Service and Maintenance eere correr 6 1 Periodic Clean cu nennt nn uns 6 1 IBS slo TET 6 1 Radioactive DesontdtullldtlO nadia 6 2 Power Supply Circuit Breaker s s tuo ss isis saba 6 2 Rotor Removal RE 6 2 Tronnion DS DEA NEL sus om GIR pleJ 6 3 Chemical Compatibility Table for Rotors
58. ulphuric Anhydride dry or moist Tetrachloroethane MoO 0 0 0 00 0M 00M 000000 0 0 0 0 0 0 0 0 U M 0 0 0 0 0 0 0 S 0 0 0 0 0 0 S U 0 Tetrachlorethylene Tannic Acid 0 Sd S 0 0 0 0 0 0 S 0 0 0 0 0 M 0 0 0 0 0 0 0 S Sd S 0 0 0 0 0 0 0 0 0 0 00 0 0 0 Tartaric Acid 0 S S 0 0 0 0 0 M M S S S S 0 0 0 0 0 U S 0 0 0 0 0 U S S M 0 Ss 0 0 0 0 0 0 S 0 0 0 0 0 S U U U U U U U U U U 0 0 U U U U U Tetrahydrofuran Thionyl Chloride Toluene P SMS 0 U 0 0 0 S 0 U U U S Trichlorethylene U 0 0 0 0 0 0 0 Mt Mt Mt Mt 00000000 S Trichloroacetic Acid 1 24 Trichlorobenzen S 0M 06 U Trichloroethane Triethylamine 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 S Sd S S S 0 0 00 0 0 0 S 0 0 Triethylene Glycol 0 S S S S 0 M 0 0 S 0 S S Tris Buffer neutral S 0 S 0 0 8 S S S S 0 M 0 0 Trisodium Phosphate Triton X 100 Turpentine S S S 0 0 00 0 0 0 S Sd U 0 0 0 0 0 Sd M 0 0 0 0 0 S 0 0 0 0 0 0 0 Sd Mt St S 0 0 0 0 0 0 0 0 Sd Sd St M 0 Undecyl Alcohol Urea S S S S S S S S S S S S S SS 0 0 0 0 0 S S 0 0 S SS 0 US Urine U 0 0 0 0 0 U U U 00000000 U Vinylidene chloride 6 9 T1 RT1 Thermo Fisher
59. y Max Min Max Radius Max Speed Max Angle Tubeg Tube Ht mm rpm RCF xg deg mm mm 11175750 Swing out rotor 6 x microplates 115 4100 2160 90 with carriers or 2 x blocks 11175631 2 sealed carriers 3 plates 1 block each 11174207 Rubber cushion for flexible microtiter plates 11210762 2 adapters for T 25 and T 75 Falcon type Cell Culture Flasks 11178216 Spare cap for plate carrier AC 15 4 High Throughput Blood Tube Rotor Cat No Description Capacity Max Min Max Radius Max Speed Max Angle Tubeg Tube Ht mm rpm RCF xg deg mm mm 11175755 Angle rotor 30 x 15 mL 17 5 100 120 135 4100 2537 37 Directly 1 x 10 15 mL 135 4100 2540 11203666 Set of 30 inserts 1x 13 5 mL 130 4100 2440 11172596 Set of 30 inserts 1 x 8 7 mL Vac 130 4100 2440 11172595 Set of 30 inserts x 5 mL Hemogard 16 4100 2180 11172402 Set of 30 inserts x5mL 16 4100 2180 11172287 Set of 30 inserts x3 mL 12 4100 2100 11172288 Set of 30 inserts x 2 1 5 mL 05 4100 1970 AC 100 10 High Capacity Angle Rotor Cat No Description Capacity MaxTube Radius Max Speed Max Angle mm mm rpm RCF xg deg 11175756 Angle rotor 6 x 100 mL 38 99 10000 11066 25 directly 1 x 94 85 80 mL 38 99 10000 11066 Thermo Fisher Scientific T1 RT1 3 6 Chapter 3 Installation 11174713 Set of 6 inserts 1x50mL 29 93 10000 10397 11174714 Set of 6 inserts 1x50 mL con 30 93 10000 10397 11174715 Set of 6 inserts 1x30 38 mL 25 5 88 1
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