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Maintenance of your LC and LC-MS System

1. 0890 1761 0890 1762 0042 6463 0890 1763 0042 6462 0890 1915 0042 6461 OD Part No m mm mm 1 6 5062 2462 3 1 0062 2483 0062 2463 0065 9978 0065 9976 0065 9971 Part No 8710 1930 87 10 1931 0062 2418 9063 6991 9042 6500 0100 1631 Fittings for 1 16 in OD Capillaries Description 1 16 in stainless steel fittings front and back ferrules 10 pk 1 16 in stainless steel long fittings front and back ferrules 10 pk 1 16 in extra long stainless steel fittings and ferrules 10 pk 1 16 in male fittings stainless steel 10 pk 1 16 in front ferrule stainless steel 10 pk 1 16 in back ferrule stainless steel 10 pk 1 16 in finger tight polyketone fittings max pressure 600 bar 10 pk 1 16 in finger tight PEEK fittings beige 10 pk 1 16 in finger tight PEEK fittings beige 2 pk 1 16 in finger tight PEEK long fittings beige 10 pk 1 16 in finger tight PEEK fittings 10 pk 2 each yellow blue black green red 1 16 in double winged 2 piece fitting PEEK RheFlex 2 piece fittings 5 pk PEEK RheFlex 2 piece fittings colored 10 pk Chromfrac identifiers 20 pk 2 each black green white 4 each yellow blue red 1 16 in blanking nut stainless steel Stainless steel hex nut fitting PEEK ferrule max 600 bar 6 pk 1 16 in PEEK ferrule max 600 bar 6 pk for use with 5067 1540 fitting 1 16 in plastic fitting plug 0 8 mm ID stainless steel ferrules 6 pk
2. Disassembling and cleaning the cell How to remove the cell 1 Remove both capillaries from the flow cell 2 Unscrew the cell screws and remove the flow cell How to disassemble the cell windows 1 Open the cell with a 4 mm hexagonal key 2 Use tweezers to remove the conical springs and gaskets 3 Remove the cell windows with scotch tape Important Do not remove the flow cell windows with tweezers If you plan to reuse them The tweezers will scratch the windows fragile surface 4 Use ethanol to clean the cell body 5 Use a cotton swab and ethanol to clean the cell windows Note if you work with proteins you may want to try a contact lens cleaner Or simply replace the windows 6 Make sure that no deposits are on the window or cell body Reassembling the cell windows and cell 1 Insert Gasket 2 Note The hole of gasket 2 is larger than the hole of gasket 1 2 Install the cell window 3 Insert Gasket 1 4 Insert the SST ring 5 Install the conical springs Note Install the conical springs with the conical side toward the cell window This will ensure correct placement of the conical springs which is critical to proper tightness 6 Carefully tighten the cell screw The cell repair kit includes the required cell windows pack of 2 and gaskets Order online at www agilent com chem store Installing the Detector Cell Before you return the flow cell to the instrument module 1 Connect the capil
3. Order online at www agilent com chem store Volume pL 10 10 25 20 50 50 100 100 250 250 500 500 Syringes for Manual Injection Valves Description Removable Fixed Removable Fixed Removable Fixed Removable Fixed Removable Fixed Removable Fixed Syringes for Manual Injection Valves Needle 22 91 3 22 91 3 22 51 3 22 91 3 22 51 3 22 51 3 22 91 3 22 91 3 22 91 3 22 91 3 22 91 3 22 51 3 The syringes for manual injection have a blunt tip point style needle to prevent damaging the valve s internal parts They can be used with any type brand of manual injection valve Part No 0182 9725 0182 9644 182 9719 0182 9628 0183 4538 0182 9619 0183 4539 0182 9613 5182 9720 9182 9624 0183 4540 0182 9658 A set of valve types specially designed for the Agilent 1100 1200 Series HPLC system allows you to extend your HPLC applications New valve offerings give you More flexibility in solvent selection and column selection New automation capabilities in sample preparation Increased sample throughput through alternating column regeneration Increased separation performance with multidimensional chromatography Switching Valve Supplies Capillary Tubing Kits Application Column regeneration Capillaries 0 17 mm ID Column regeneration Capillaries 0 25 mm ID Column regeneration Capillaries 0 17 mm ID Column selection Capillaries 0 17 mm ID Sample enrichment Capil
4. Precise alignment Note The new C type detectors use lamps with a chip installed to automatically count Thermal stability Boeri panje e Easier handling during installation and removal 66 Order online at www agilent com chem store Deuterium lamp Replacement Parts Description Deuterium lamp 1100 DAD MWD For G1315C DAD and G1365C MWD For G1315A B DAD and G1365A B MWD Tungsten lamp assembly For G1315A B DAD and G1365A B MWD Holmium Oxide filter Column connecting capillary with fittings 150 x 0 17 mm Order online at www agilent com chem store Part No 2140 0590 Deuterium lamp 1100 DAD MWD long life 2140 0813 Deuterium lamp 1100 DAD MWD long life with ID tag 2140 0820 0181 1530 Deuterium lamp 1100 DAD MWD longlife G1103 60001 0022 2184 79880 22711 G1315 45001 G1315 8 311 G1315 8 312 G1315 87303 Universal ZDV union stainless steel no fittings Capillary Nano Standard LC Lever for Holmium Oxide Filter Column connecting capillary with fittings 380 x 0 17 mm Column connecting capillary with fittings 150 x 0 12 mm 67 68 Order online at www agilent com chem store G1315 60011 Semi Micro Flow Cell and Replacement Parts Item k o lt o cd AI Wy N Description Semi micro flow cell 6 mm 5 uL 120 bar Semi micro flow cell with ID tag 6 mm 5 uL 120 bar Micro flow cell with ID tag 3 mm 2 uL 120 bar Window assembly inc
5. 0 8 mm ID stainless steel fittings M4 4 mm 6 pk Order online at www agilent com chem store Part No 0062 2418 0065 4454 0065 9967 0061 3303 0180 4108 0180 4114 0042 8957 0063 6591 0100 1516 0062 8541 0065 4426 0042 6500 0100 1631 0100 2175 0350 1402 01080 83202 0067 1540 0067 1547 0100 1259 0067 1557 0067 1558 81 Unions Description Use With Part No True ZDV Union no fittings Nano LC 0022 2145 Universal ZDV union stainless steel Capillary Nano Standard LC 9022 2184 no fittings ZDV union with fittings Standard LC 0100 0900 ZDV union PEEK with fittings Bio applications 0100 2441 High flow union no fittings Prep LC 9022 2133 PEEK adapter 1 4 28 to 10 32 0100 1847 Adapter PEEK int 1 4 28 to ext 10 32 0100 2298 Barbed Y Connector PP for 3 16 in ID tube 10 pk 0065 9971 0100 0900 Adapter female to female 1 4 28 0042 8517 Adapter male luer to female 1 4 28 0042 8518 Micro T connector PEEK swept vol 29 nL with 1 32 in ID fittings 0042 8519 0022 2133 LC Connecting Capillaries Universal Connection Capillaries e Made of flexible stainless steel 0 6 mm OD with 1 16 in OD tubing at both ends to accept standard fittings e Pre swaged fittings are assembled according to Swagelok specifications 82 Order online at www agilent com chem store Universal Connection Capillaries From Pump Autosampler Manual valve Column compartment Detector Column VWD SS stainless
6. 2 cell seal assemblies 5 litetouch front and back ferrules Parts for the G1315 68716 80 nL Flow Cell Description Fused silica PEEK capillary 50 um ID 40 cm long Inlet Fused silica PEEK capillary 50 um ID 12 cm long Outlet Fused silica PEEK capillary 25 um ID 20 cm long Inlet Fused silica PEEK capillary 25 um ID 60 cm long Outlet Sealing kit for 80 nL flow cell Includes torque adapter 2 cell seal assemblies 5 litetouch front and back ferrules 5 sleeves for 360 um OD capillaries Part No G1315 87333 G1315 87323 G1315 87338 G1315 87328 G1315 68715 Part No G1315 87323 61315 87328 G1315 87313 G1315 8 318 G1315 68725 G1315 60017 18 Preparative Flow Cells and Replacement Parts Description Prep Flow Cell Quartz 0 3 mm 20 bar Prep Flow Cell Quartz 0 06 mm 20 bar PTFE tubing 0 8 mm ID 2 m PTFE tubing 0 5 mm ID 0 8 m Cell housing Quartz body 0 3 mm Quartz body 0 06 mm Prep Flow Cell SS 3 mm 120 bar Stainless steel connecting capillary 0 5 mm 250 mm Order online at www agilent com chem store Part No G1315 60017 G1315 60018 G1315 67301 G1315 67302 G1315 27 05 G1315 80004 G1315 80003 G1315 60016 G1315 87305 71 Agilent is a world wide leader in mass spectrometry with powertul reliable LC MSD solutions Agilent mass spectrometers work seamlessly with our industry leading LC separation technologies and columns to provide exceptional sensitivity analytical perfo
7. Maintenance Function e Remove the front cover when the needle is positioned approximately 15 mm over the needle seat a sl 1 Disconnect the seat capillary from the injection valve port 5 2 Use a small flat screwdriver to ease out the needle seat 3 Insert the new needle seat assembly Press the seat firmly into position Connect the seat capillary fitting to port 5 of the injection valve 4 Use the Needle Down button to position the needle above the seat making sure the needle and seat are properly aligned Install the front cover and select END under Maintenance Function Order online at www agilent com chem store 17 18 1100 1200 Needles and Needle Seats Agilent Autosampler Needle Assembly Description G1313A G1327A G1329A Needle assembly standard autosampler G1313A G1327A G1329A Needle assembly for use with PEEK seat G1313A G1327A G1329A Needle assembly 900 uL upgrade G1387A G1389A Needle assembly micro LC autosampler Part No G1313 8 201 G1313 87203 G1313 87202 G1329 80001 G136 A Needle assembly well plate autosampler G1367 87200 green Needle assembly well plate autosampler 136 87201 new blue G13 A Needle assembly micro well plate sampler G1377 87201 G2258A Needle assembly dual loop autosampler G2258 687 10 G2260A Needle assembly prep autosampler G2260 87201 Order online at www agilent com chem store G2260 87201 Compatible
8. Semi Prep Filter 0 5 12 7 1 5 mL min 9064 8273 5022 2185 Semi Prep Filter 10 19 5 10 mL min 5022 2165 5022 2166 10 pk Prep Filter 10 10 100 mL min 9065 4500 5065 9901 Replacement Glass Cartridge In line filter for G1311A Recommended when high salt concentrations are used G1311 60006 Order online at www agilent com chem store 55 Vacuum Degassers G1322A 1100 Series Vacuum Degasser Description Part No Online degasser accessory kit G1322 68705 Includes 8 screws 8 bushings 4 markers tubing syringe syringe adapter Glass filter solvent inlet 20 um 0041 2168 Disposable syringes 20 mL 0062 8534 Syringe Adapter 1 16 in OD 2 in long 9301 1337 Tetzel Ferrules and SS Lock Rings 1 8 in 0063 6598 PPS nuts 1 8 in 1 4 28 thread 9063 6599 Union 1 4 28 threads Polypropylene 0022 2155 Teflon Solvent tubing 0062 2483 5063 6598 S Tubing kit degasser to pump G1322 67300 4 pk 30 cm pieces of tubing with screws and bushings Mounting tool for flangeless nut 0100 1710 as Plastic tubing cutter 8710 1930 G1379A B 1100 1200 Series Micro Vacuum Degasser 5063 6599 Description Part No Dual channel micro degasser vacuum chamber G1379 60010 Micro vacuum degasser tubing kit for G1379A G1379 67310 Micro vacuum degasser tubing kit for G1379B 0042 8922 Mounting tool for flangeless nut 0100 1710 0100 1710 T 56 Order online at www agilent com chem store Detector Maintenance Ensure uncompromised chromatograp
9. e Electronic crimpers and decappers These supplies are also designed for fast easy replacement so you can get your system running again quickly Order online at www agilent com chem store 71 O Syringe Econofilters 5185 5830 Ready to Use Econofilter Membrane Syringe Filters Agilent s industry standard 25 mm diameter Econofilters are an ideal choice for high sample throughput laboratories We recommend using them to clarify small volume HPLC samples that need further analysis or when particulate matter in the sample solution may cause problems Features include e The same quality membranes found in Agilent s high performance syringe filters e An inert polymeric housing that helps to spread the sample over the membrane s entire surface so that the maximum capacity is reached e No glue or binders used during construction which ensures that no extractables are present e Built in simplicity just attach the Luer tipped syringe filled with the sample solution to the housing and push the sample through the pre cleaned filters Econofilters have a moderately wide cross sectional area 4 2 cm with a holdup volume of less than 50 microliters Popular pore sizes of 0 20 and 0 45 micron are available in packages of 200 units Regenerated cellulose nylon and PTFE polytetrafluoroethylene membranes are also available in these configurations Membrane Econofilters 200 pk Description Diameter
10. 0022 6509 0022 6510 0065 9926 Part No 9061 3304 0061 3315 Starter Kits Description Contents Part No Agilent starter kits contain the most often Capillary fitting starter Kit includes 5065 9939 used capillaries and fittings We included kit 0 18 mm ID Oty 1 PEEK capillary 0 18 mm ID 1 5 m our genuine flexible stainless steel Qty 4 Stainless steel capillary 105 x 0 17 mm capillaries to make the best connection in Oty 4 Stainless steel capillary 150 x 0 17 mm your LC system no matter the brand The Oty 2 Stainless steel capillary 200 x 0 17 mm kits are for use with 3 4 or 1 2 mm ID Oty 2 Stainless steel capillary 280 x 0 1 7 mm columns as well as for Micro LC columns Qty 1 Stainless steel capillary 400 x 0 17 mm Tubing cutter for PEEK capillaries 1 16 in stainless steel fittings 10 pk 1 16 in PEEK fittings color 10 pk 1 16 in PEEK fittings 10 pk Qty 3 ZDV union stainless steel The tree Cybertool which contains over 30 tools is useful in every laboratory Rheotool Cybertool Capillary fitting starter Kit includes 0065 9937 kit 0 12 mm ID PEEK capillary 0 13 mm ID 1 5 m Qty 4 Stainless steel capillary 105 x 0 12 mm Qty 4 Stainless steel capillary 150 x 0 12 mm Qty 2 Stainless steel capillary 200 x 0 12 mm Qty 2 Stainless steel capillary 280 x 0 12 mm Stainless steel capillary 400 x 0 12 mm Tubing cutter for PEEK capillaries 1 16 in stainless steel fittings 10 pk 1
11. 10 pk Order online at www agilent com chem store Part No 3150 0577 0188 2743 0188 2744 0188 2745 0188 2746 3150 0576 9301 0895 3150 0509 51 Cleaning or Replacing the Solvent Inlet Filters Clean or replace the solvent inlet filters at least once every 3 months Stainless steel filters can be sonicated in isopropanol however glass filters cannot be sonicated as glass particles may break off or the filters may shatter To test if a solvent inlet filter is blocked disconnect the solvent inlet tube at the end furthest from the filter and bottle head assembly If the filter is in good condition solvent will flow freely out of the solvent tube If there is a blockage little or no solvent will drip from the tube If a blockage occurs it s easiest to simply replace the solvent inlet filter However you can clean a glass filter by using the following procedure 1 Remove the solvent inlet filters 2 Replace the glass frit filters or soak them in concentrated nitric acid 35 for one hour Be sure to flush well to avoid column damage 3 Install either the new or cleaned solvent filters Order online at www agilent com chem store Solvent Filters for G1311 12 54A Pumps Unfiltered solvents or so lvents contaminated with microbial growth Filter frit aE Hi 2 851 406 can clog solvent inlet filt the lifetime of the filter and impacting pump performance Remember to clean or replace the solvent in
12. 16 in PEEK fittings color 10 pk 1 16 in PEEK fittings 10 pk Oty 3 ZDV fitting Rheotool Cybertool Capillary fitting starter Kit includes 0065 9938 kit for 1100 Capillary Qty 2 Fused silica PEEK capillary 50 um 55 cm LC System Qty 1 Fused silica PEEK capillary 50 um 20 cm Qty 1 Fused silica PEEK capillary 100 um 110 cm Qty 2 Fused silica PEEK capillary 50 um 50 cm Qty 2 Fused silica PEEK capillary 50 um 40 cm Qty 4 4 mm stainless steel fitting male 10 32 Qty 4 1 32 in PEEK ferrule and stainless steel lock ring Qty 4 PEEK fittings for y valves Qty 4 Double winged PEEK nuts and 1 32 in ferrules Qty 1 Cybertool Rapid Resolution High Kit includes 0065 9947 Throughput capillary kit Qty 5 Capillaries to optimize the 1100 Series LC for use with RRHT columns 1200 capillary kit for Includes capillaries to optimize 1200 LC for low G1316 68716 0 12 mm ID dispersion operation Capillary and fitting starter kit Order online at www agilent com chem store 85 86 From Pump Damper Damper Mixer Cell out To Autosampler Cooled Autosampler Pressure sensor Mixer Outlet Valve MS DAD heat exchanger capillary DAD heat exchanger capillary Valve Micro Valve WPS Micro valve ICE VWD Column WPS Cooled WPS Column Micro valve Column 0 8 mm OD stainless steel capillaries use 0 8 mm ID fittings Valve Regeneration Pump Micro valve Heat exc
13. 87611 G1313 87305 01090 87304 01048 87302 01090 87305 G1328 87600 G1316 87303 G1316 87300 G1315 87312 G1315 8 311 0062 8522 0021 1820 0021 1821 9021 1822 9021 1823 G1316 87303 0021 1816 0021 1817 0021 1818 0021 1819 61316 87300 0062 8535 83 84 ID mm 0 12 0 12 0 12 0 12 0 12 0 12 0 17 0 17 0 17 0 17 0 17 0 17 0 17 0 17 0 25 0 25 0 25 0 25 0 50 0 50 0 50 0 50 Length mm 105 150 200 280 400 500 105 150 200 280 400 600 700 900 250 280 320 800 105 150 280 800 Capillary and Fittings Kits Order online at www agilent com chem store Description SS Flexible Capillary Tubing Kit Includes 0 12 mm ID capillaries 3 each 105 mm long 1 each 150 mm long 1 each 280 mm long plus fittings SS Flexible Capillary Tubing Kit Includes preswaged 0 12 mm ID capillaries 3 each 70 mm long 1 each 280 mm long 1 each 35 mm long Color Code Red Red Red Red Red Red Green Green Green Green Green Green Green Green Blue Blue Blue Blue None None None None Flexible Stainless Steel Capillaries Without Fittings Volume pL 1 2 led 2 3 3 2 4 5 0 6 2 4 3 4 4 6 6 4 9 1 13 6 15 9 20 5 12 3 13 8 15 8 39 3 20 6 29 0 D9 157 Part No 0021 1820 0021 1821 0065 9935 0021 1822 0021 1823 0065 9964 0021 1816 0021 1817 0065 9931 0021 1818 0021 1819 0065 9933 0065 9932 0065 9963 0065 9979 0022 6508 0065 9980 0065 9930 0065 9927
14. G1315 87303 G1316 87316 G1316 8731 G1316 87318 G1316 87319 G1316 87321 G1316 87323 G1316 8 326 G1316 8 32 General Supplies SSV Pumphead A Pumphead B Column d etering I evice f y Needle Generic Connecting Capillaries for Capillary LC System Item From To Fitting Part No 1 SSV AIV G1311 67304 2 OBV Piston 2 A A G1312 67300 3 Pump Restriction capillary A A G1312 6 302 4 Mixing capillary Damper A A G1312 6 304 0 Damper Mixer A A 01090 87308 6 Mixer Filter A A 01090 87308 I Filter EMPV A A G1375 87400 8 Needle seat Injection valve C G1329 8 101 Order online at www agilent com chem store 87 88 Order online at www agilent com chem store PEEK Coated Fused Silica Capillaries for use with 20 pL min Flow Range Item a b From EMPV Flowsensor Injection valve Metering device Injection valve Injection valve Column Detector U switching valve To Flowsensor Injection valve Metering device Needle Column Waste Detector Waste Column PEEK Coated Fused Silica Capillaries for use with 100 pL min Flow Range Item From a EMPV b Flowsensor Injection valve Metering device Injection valve Injection valve Column Detector To Fitting Flowsensor B B Injection valve B C Metering device B C Needle B B Column C D Waste C Detector D E Waste E C D u switching valve Column Fitting B B B C B C B B C D C D E E C D ID um 5
15. General Supplies Part No 0062 2418 9063 6593 0065 4423 0065 4410 0065 4422 0022 6536 Part No G13 5 8 320 G13 5 8 321 G13 5 8 322 G13 5 8 323 G13 5 8 324 G13 0 8 325 G13 5 8 32 G2228 68 00 89 Certified Vials Caps and Septa Agilent s certified vials are manufactured with the same high quality design technical expertise and exacting specifications that go into every Agilent instrument Every order of certified vials caps and septa comes with a test certificate confirming product specifications Certified Vials e Compatible with Agilent autosamplers e Made from first hydrolytical class borosilicate glass type 1 e Manufactured in a ISO 9001 certified environment e 100 automated computerized video imaging to test critical dimensions including vial finish outer diameter body OD and length e Packed in a clean environment and with packaging especially designed to reduce contamination Certified vials and caps e Compliant with requirements of U S and European Pharmacopoeia Certified Caps and Septa e Designed and fabricated for proper sealing and trouble free operation with Agilent instruments e Manufacturing SOPs monitored stringently for consistent quality and full traceability e Chromatographic test on silicone septa for purity to eliminate outliers and Sample errors 90 Order online at www agilent com chem store Screw top vials and caps Vials Descriptio
16. SL pump 600 bar Purge valve assembly 600 bar Seal keeper ceramic Stainless steel capillary 400 x 0 17 mm m m ps ps pump to autosampler Stainless steel capillary 700 x 0 17 mm m m ps ps pump to cooled ALS Stainless steel capillary 150 x 0 17 mm m m ps ps damper to pressure sensor Stainless steel capillary 105 x 0 17 mm m m ps ps damper to mixer or mixer to outlet valve Calibration capillary assembly Absorber capillary 500 uL volume Stainless steel restriction capillary 0 17 mm ID T piece to pressure sensor Solvent mixer short 200 uL G1376A 1100 Series Capillary Pump Description Capillary pump accessories kit Capillary pump PM kit Semi prep filter Replacement frits 0 5 jim for P N 5064 8273 Torque wrench adapter Bottle head assembly with tubing and filter Solvent inlet filter stainless steel Stainless steel capillary pump ALS 90 cm 0 17 mm ID Extended flow range kit 100 pL min Order online at www agilent com chem store Part No 61312 68730 G1312 60025 61312 60020 G1312 60022 61312 60023 0042 8586 61312 87303 61312 87304 61312 87305 G1312 87306 G1312 67500 61312 87300 G1312 87301 0067 1565 Part No G13 6 68 05 G137 6 68 10 0064 8273 0022 2185 61315 45003 G1376 60003 01018 60025 G1329 87300 G13 6 68 07 47 G1361A 1100 1200 Series Prep Pump Description Accessory kit prep pump gradient G1361A bottle head assembly filter glass stop valve stainless s
17. Window UHP 2 pk Part No G1314 60087 G1314 60081 G1314 65052 79853 27200 79853 29100 79853 22500 79853 68742 79853 68743 G1314 65053 Part No G1314 60082 G1314 65054 79853 27200 79853 68734 Order online at www agilent com chem store 63 Diode Array Detector DAD Multiple Wavelength Detector MWD DAD Routine Maintenance Procedures e Perform the intensity test and detector cell test These built in tests will help you verity the performance of your detector and determine when maintenance Is necessary e Clean or replace the flow cell windows as needed e Perform a wavelength calibration to ensure wavelength accuracy e Clean the leak sensors and check their function e Inspect the positioning of the waste tube e Exchange the lamp Perform a dark current and filter test Diode Array Detector DAD Flow Cell Maintenance Tips Step 1 Remove the capillaries Step 2 Take out the cell Step 3 Remove the cell screw and gasket 64 Order online at www agilent com chem store Flow cell Choosing the Optimal Flow Cell Agilent offers eight flow cells for use with the diode array and multiple wavelength detectors Use the chart below to select the appropriate one for your application keeping the following guidelines in mind DAD MWD Flow Cell Selection Typical Column Typical Peak Length cm Width Typical Flow Rate Internal Column Diameter If more than one
18. a wide selection of fixed needle 22 gauge Pointstyle 3 syringes to accommodate a variety of applications X type syringe and plungers Volume pL 25 50 29 100 290 000 1000 2900 0000 Description X type Fixed Needle Replacement plunger for X type syringe X type Fixed Needle Replacement plunger for X type syringe X type Fixed Needle fast aspiration and eject X type Fixed Needle low dead volume Replacement plunger for X type syringe Fixed Needle Replacement plunger Fixed Needle Replacement plunger Fixed Needle Fixed Needle Replacement plunger Fixed Needle Replacement plunger Fixed Needle Replacement plunger Fixed Needle Replacement plunger Fixed Needle Replacement plunger Fixed Needle Replacement plunger Unit 3 pk 3 pk 3 pk 10 pk 10 pk 10 pk 10 pk Order online at www agilent com chem store HTS and HTC PAL Liquid Injection Syringes Gauge 22 22 22 22 22 22 22 22 22 22 22 22 12 Needle 228 91 3 22 51 3 225 91 35 228 51 3 225 91 53 22 51 3 223 51 3 22 91 3 22 51 3 22 91 3 22 51 3 22 51 3 Part No G4200 80117 G4200 80112 0188 6485 0188 9395 G4200 80118 G4200 80119 G4200 80111 G4200 80113 G4200 80103 G4200 80114 G4200 80104 G4200 80115 G4200 80116 G4200 80105 G6500 80102 G4200 80102 G6500 80103 G4200 80106 G6500 80104 G4200 80101 G6500 80105 G4200 80107 G6
19. at www agilent com chem store J 1 Remove the capillaries from the stator face Remove the stator screws with a 9 64 Allen wrench 2 Remove the stator head stator face and stator ring 3 Remove and replace the rotor seal and the isolation seal if necessary 4 Install the stator head then the stator face Replace the stator screws tightening them evenly and caretully 5 Connect all capillaries according to the diagram A Pump D Waste B Metering device E Needle seat C Plug F Column Injection Valve Maintenance Use With G1313A G1329A G1367A G136 C G137 A G2258A G2260A Stator face ceramic Description 2 Position 6 Port Injection Valve 2 Position 6 Port Injection Valve 600 bar 2 Position 6 Port Micro Injection Valve 10 Port Dual Loop Valve 2 Position 6 Port MBB Injection Valve Part No 0101 0921 0101 1422 0101 1050 0101 1385 0101 1267 RheBuild Kit Part No 0101 1257 0101 1257 0101 1268 Rotor Seal Part No 0100 1853 Vespel 0100 1849 Tefzel 0100 2231 PEEK 0101 1416 0100 2088 Vespel 0100 2415 Stator Part No 0100 1850 Stator head 0100 1851 Stator face 0101 141 0100 2089 0101 1390 Order online at www agilent com chem store 15 Before beginning this procedure e Select Change Needle under Maintenance Function e Remove the front cover when the needle is positioned approximately 1
20. jen je seje ee a ne ee a aa een ee 82 GTi AULT RR je Ee 6 oes ae E A A MA nena 90 Electronic Crimpers and ECAP DENS ie ano eo oka ooo ei ao peak 95 Aent EESO aa a 96 Welcome to the next generation of HPLC The new Agilent 1200 Series is designed for ultimate robustness reliability and performance No matter what your application may be the bottom line is results And the new Agilent 1200 Series platform helps you achieve the best results with features such as e Intuitive user interfaces that let you control and monitor instrument status e Newer and more efficient data analysis and review tools e Enhanced diagnostic software that detects small problems before they lead to costly downtime In addition Agilent s 1200 Series LC platform helps you achieve all the performance all the time with a flexible modular design that allows you to configure the system to meet your unique application requirements And this maintenance guide will help you keep your Agilent 1200 Series running at optimal performance and peak efficiency Inside this guide you ll find e Essential facts about Agilent supplies and accessories e Vital maintenance procedures and troubleshooting tips e All the Agilent part numbers you need in one easy to use guide Order online at www agilent com chem store 1 Agilent ZORBAX Rapid Resolution High Throughput RRHT columns Boost your productivity without sacrificing performance reliabilit
21. male male Stainless steel capillary EMPV to next module 0 6 x 400 mm male male Stainless steel capillary EMPV to multi assembly 0 5 x 160 mm male male Stainless steel capillary union to EMPV2 male female Stainless steel capillary union to mixer 0 6 x 40 mm male female Stainless steel capillary EMPV1 to union male female Manual prep injection valve kit stainless steel With position sensing 10 mL loop 25 mL syringe ring mounting bracket start cable SS connecting capillaries 0 5 mm ID 40 cm and 60 cm Glass stop valve assembly Includes stop valve and 2 adapters Inlet tubing stainless steel for pressurized solvent Order online at www agilent com chem store Part No 0065 4421 9301 6341 9301 6342 G1361 60022 61361 22402 0022 2188 G1361 23204 0065 4445 G1361 60012 G1361 67300 G1361 67301 G1361 67302 61361 67303 G1361 67304 G1361 67305 G1361 67306 0065 9922 0065 9909 G1361 60008 49 Solvent Filtration Untiltered solvents or solvents contaminated with microbial growth especially aqueous buffer solutions present a common problem in HPLC Aqueous media encourage microbial growth that can clog the solvent inlet filter and the HPLC particulates in untiltered solvents can have the same affect Both microbial growth and particulates can also impact the performance of the pump For instance blocked solvent inlet filters can cause erroneous mobile phase composition which will impact peak
22. mm Pore Size um Part No Regenerated Cellulose ooo 25 Oooo 0 2 O 5185 5830 Regenerated Cellulose 25 0 45 0185 5831 Nylon 20 0 2 0185 5832 Nylon 29 0 45 0185 5833 PTFE 29 0 2 0185 5834 BIFE 20 0 45 0185 5835 78 Order online at www agilent com chem store PEEK tubing LC Tubing and Fittings What is the Function of LC tubing LC tubing creates the sample flow path through your LC system from the autosampler Or manual injection valve through the column to the detector The ideal tubing provides an inert surface leak free connections and zero dead volumes What advantages does Agilent tubing offer Agilent stainless steel and PEEK tubing each provide their own unique benefits e Stainless Steel Tubing is precisely measured and pre cut according to Agilent s exact specifications and is available in universal lengths Additionally Agilent capillary tubing is color coded based on internal diameters e 1 16 in OD PEEK Tubing is flexible and easy to cut to desired lengths It s also color coded for easy tracking Additionally PEEK tubing accepts both stainless steel and PEEK fittings When should replace the LC tubing You should inspect your system periodically and replace the tubing when you notice the following e Leaking or loose fittings e Pinched or constricted tubing e Application changes How can minimize problems with my LC tubing e Choose the narrowest diameter and shortest length that
23. selection is appropriate choose the larger flow cell to get the best detection limit For columns capable of providing narrower peak widths 1 8 um particle size the smaller flow cell may be more appropriate Flow cells with longer path lengths provide better signal detection However a longer path length also increases the cell volume which causes more peak dispersion For information on selecting prep cells please refer to our technical note G1315 90102 under Library Information at www agilent com chem Recommended Flow Cell High Pressure Flow Cell Standard Flow Cell Order online at www agilent com chem store 65 Changing the Deuterium Lamp You should replace the deuterium lamp when the baseline noise and or drift exceed your application limits or if the lamp does not light Use the following procedure 1 If the detector has been in use turn it off and allow it to cool before proceeding 2 Remove the front cover 3 Disconnect the electrical connection and unscrew the lamp to remove it from the DAD 4 Replace with a new lamp taking care not to touch the glass surface with your hands Agilent s DAD lamp can help you achieve optimal performance with these 5 Tighten the screws and reconnect the lamp to its electrical connection innovative features 6 Replace the front cover A lifetime of over 2000 hours 7 Reset the lamp counter and turn on the lamp Allow it to warm up for 10 20 minutes
24. steel To Autosampler Manual Valve Universal Column Column Column Waste DAD Inlet Outlet ID mm 0 17 0 25 0 25 0 17 0 17 0 25 0 12 0 12 0 12 0 17 0 17 0 17 0 17 0 17 0 12 0 17 0 12 0 17 0 18 0 12 0 12 0 12 0 12 0 12 0 17 0 17 0 17 0 17 0 17 0 25 Length mm 600 130 320 900 900 700 180 280 105 180 280 800 130 500 70 90 150 380 40 105 150 280 400 70 105 150 280 400 90 Material SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS SS PEEK SS SS SS SS SS SS SS SS SS SS PEEK Color Code Green Blue Blue Green Green Blue Red Red Red Green Green Green Green Green Red Green Red Green Red Red Red Red Red Green Green Green Green Green Order online at www agilent com chem store Fittings Pre swaged Pre swaged Pre swaged With fittings Not swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged With fittings 1 end pre swaged With fittings Pre swaged Pre swaged With fittings Without fittings Without fittings Without fittings Without fittings 1 end pre swaged Without fittings Without fittings Without fittings Without fittings With fittings With fittings General Supplies Part No G1312 67305 01090 87308 79835 87638 G1328 87600 G1329 87300 01018 67305 G1313 87304 01090 87610 01090
25. your application and system will allow This will help prevent peak dispersion or resolution loss e Select smaller ID tubing 0 12 mm for narrowbore or microbore applications e Use color coded tubing or Chromlrac identifiers to facilitate maintenance and troubleshooting How do choose the correct fittings To ensure leak free connections and to prevent the loss of peak shape and resolution always use the manufacturer s recommended fitting style with columns valves and unions For example e Different columns have different fitting requirements ZORBAX columns use standard Swagelok fittings e Agilent 1100 1200 modules use standard Swagelok fittings e Rheodyne injection valves require Rheodyne fittings Order online at www agilent com chem store 19 PEEK Tubing Length m ID mm Color Code 1 5 0 50 Orange 1 5 0 25 Blue D 0 25 Blue 1 5 0 18 Yellow g 0 18 Yellow Lo 0 13 Red O 0 13 Red Other Tubing 8710 1930 Description Length ID Teflon tubing FEP primary use for valve solutions F 0 7 Teflon Solvent tubing primary use for flow path from O 1 5 solvent bottle to degasser to pump Corrugated tubing polypropylene O 6 5 Silicone Tubing 1 mm ID 3 mm OD 5 m O 1 Clamps and micro clamps 10 pk Barbed Y Connector PP for 3 16 in ID tube 10 pk Accessories 80 Order online at www agilent com chem store Description Plastic tubing cutter Blades for plastic tubing cutter 5 pk Part No
26. 0 50 50 100 50 100 50 75 50 ID um 100 100 100 100 75 100 19 79 50 Part No G13 5 87301 G13 5 8 310 G13 5 8 302 G13 5 8 303 G13 5 87304 G1375 87307 G1315 68703 G1315 68708 G13 5 8 309 Part No G13 5 87 305 G13 5 87306 G13 5 8 312 G13 5 87303 G13 5 8 311 G13 5 8 30 G13 5 87308 G1315 68708 G13 5 87309 Replacement Fittings and Ferrules for Capillary and Nano Flow System Graphic Buu Description 1 16 in stainless steel fittings front and back ferrules 10 pk 1 16 in stainless steel fittings male 4 mm 10 pk 1 32 in ferrule and stainless steel lock ring 10 pk 6 fittings 2 plugs PEEK for ji valves s Double winged PEEK nuts and 1 32 in ferrules 10 pk PEEK fitting long for 1 32 in OD capillary PEEK Coated Fused Silica Capillaries for Nano LC Description 25 um ID 25 um ID 25 um ID 25 um ID 25 um ID 90 jim ID 75 um ID 10 cm long 22 cm long 39 cm long 55 cm long 70 cm long 10 cm long 65 cm long connecting valve column connecting EMPV flow sensor connecting flow sensor injection valve connecting valve flow sensor or column connecting valve column connecting valve column connecting valve injector seat or 2nd pump Nanoflow LC start up kit Includes PEEK coated fused silica capillaries column and fittings to start up a Nanoflow LC system Order online at www agilent com chem store Type A
27. 0 25 mm ID Both types should be replaced when they become contaminated worn or damaged Before replacing the tubing be sure to 1 Position the fraction collector s transport unit in the Home position 2 Remove all installed trays from the tray base 3 Position the transport unit in the Change Parts position 4 Turn off the instrument Consult your user s manual for detailed replacement instructions Analytical and Preparative Fraction Collector Maintenance Fraction collectors spotters are very user friendly and do not require much maintenance However you should perform the following routine procedures e Exchange the tubing at least once per year e Replace the capillaries e Replace the flap septum Simple Repairs Procedure When to Perform Replace the inlet waste tubing Once per year or when you notice signs of damage or wear Replace the valve to needle tubing Once per year or when you notice signs of damage or wear Exchange the preparative needle assembly When the needle shows signs of damage or blockage Exchange the analytical needle assembly When the needle shows signs of damage or blockage or when using the short needle assembly with high test tubes gt 45mm Exchange the diverter valve When the valve is leaking or not switching properly Exchange the internal tray When the flow delay sensor no longer works Repair or exchange a funnel within the When defective leaky blocked int
28. 00 hours G1314 60100 Deuterium longlife lamp 2140 0813 VWD inlet tubing with fitting 0 18 x 40 mm PEEK 0062 8522 VWD outlet tubing with fitting 0 25 x 48 mm PEEK 0062 8535 Check manual for proper setting of detector 58 Order online at www agilent com chem store Choosing the Optimal Flow Cell For Your Variable Wavelength Detector i WE Agilent offers four flow cell options for the VWD Use the chart below to select the appropriate one for your application keeping the following guidelines in mind Flow cell e f more than one selection is appropriate choose the larger flow cell to get the best detection limit e For columns capable of providing narrower peak widths 1 8 jim particle size the smaller flow cell may be more appropriate e Flow cells with longer path lengths provide better signal detection However a longer path length also increases the cell volume which causes more peak dispersion Agilent offers four flow cell options for the VWD Use the chart below to select the appropriate one for your application keeping the following guidelines in mind VWD Flow Cell Selection Typical Column Typical Peak Length cm Width Recommended Flow Cell Micro Flow Cell 138 0 05 0 2 mL min Semi micro Flow Cell 60 High Pressure Flow Cell For Pressure Above 100 bar Standard Flow Cel Typical Flow Rate Internal Column Diameter Order online at www agilent com chem store 59
29. 05 0183 4508 0182 0545 0183 4506 0183 4509 0182 0567 9301 0978 Vials with Fixed Inserts Description Unit Part No Screw top glass vial with fixed insert 100 pk 5188 6591 Screw top glass vial with fixed insert amber 100 pk 5188 6592 Crimp top micro vial with fixed insert 100 pk 9301 1388 Crimp top vial with fixed insert amber 100 pk 5188 6572 Snap top glass vial with fixed insert 100 pk 5188 6593 Snap top glass vial with fixed insert amber 100 pk 5188 6594 High Recovery Glass Vials Description Unit Part No Screw top vial high recovery 100 pk 5183 2030 Screw top vial high recovery amber 100 pk 5183 2073 Crimp top vial high recovery 100 pk 5182 3454 Crimp top vial high recovery silanized 100 pk 5183 4497 RES Snap top vial high recovery 100 pk 5183 4510 Order online at www agilent com chem store 93 Caps Description Unit Part No Polypropylene Wide Opening Screw Caps with Septa Blue PIFE red silicone rubber septa 100 pk 5182 0717 Green PTFE red silicone rubber septa 100 pk 5182 0718 Red PIFE red silicone rubber septa 100 pk 5182 0719 Blue PTFE silicone septa 100 pk 5182 0720 Green PTFE silicone septa 100 pk 5182 0721 Red PTFE silicone septa 100 pk 5182 0722 Crimp Caps with 11 mm Septa Silver aluminum clear PIFE red rubber septa 100 pk 5181 1210 Silver aluminum clear PIFE red rubber septa 1000 pk 5183 4498 Blue aluminum clear PTFE red rubber septa 100 pk 5181 1215 Green aluminum clear PTFE re
30. 1243 8 Order online at www agilent com chem store PEEK Sample Loops PEEK Is inert to almost all organic solvents and is biocompatible Like metal loops the actual volumes of PEEK loops can differ from their nominal designations because of the 0 05 mm 0 002 in tolerance of the tubing bore Generally accuracy rates are about 14 for large PEEK loops 0 8 mm 0 030 in 21 for intermediate loops 0 5 mm 0 020 in and 65 for small loops 0 2 mm 0 007 in Although PEEK is compatible with virtually all solvents many factors can affect the burst pressure of PEEK tubing For example wall thickness temperature exposure time and concentration of organic solvents can all impact durability Additionally solvents such as THF methylene chloride and DMSO cause PEEK to swell while concentrated nitric acid and sulfuric acid can weaken PEEK tubing PEEK sample loops PEEK Sample Loops for 9725 Injectors Description o uL 10 uL 20 pL 90 uL 100 uL 200 uL 500 pL 1 mL 2 mL o mL ID mm Part No 0 18 0101 1241 0 25 0101 1240 0 25 0101 1239 0 51 0101 1238 0 51 0101 1242 0 51 0101 1237 0 76 0101 1236 0 76 0101 1235 0 76 0101 1234 0 76 0101 1230 PEEK Sample Loops for 3725 Injectors Description 2 mL o mL 10 mL 20 mL ID mm Part No 1 6 0101 1229 1 6 0101 1228 2 0 0101 1227 2 0 0101 1226 Order online at www agilent com chem store 9 10 Syringes for manual injection valves
31. 150 mm Fused silica PEEK with fittings Seat Capillary 75 um 150 mm Fused silica PEEK with fittings Needle assembly micro well plate sampler Loop capillary 8 uL Loop capillary 40 uL SS capillary 0 25 mm ID 12 cm long Connecting Rheodyne valve waste Tool for micro seal capillary mounting Part No G13 68 05 G13 5 87300 G13 5 8 31 G13 5 8 316 G13 7 87201 G13 5 8 315 G13 87300 G13 87301 G13 44900 Well Plates Description 96 Well plates 0 5 mL polypropylene 2 96 Well plates 0 5 mL polypropylene it 96 Deep well plates 1 mL polypropylene Closing mats for 96 well plates silicone G2255 68700 96 Well Plates 150 uL conical polypropylene 384 Well plates 90 uL polypropylene 96 Deep well plate with glass inserts 0 35 mL caps septa Glass inserts 350 uL Caps septa for glass inserts Vial plate for 54 x 2 mL vials 5022 6539 Tray for 27 Eppendorf safe lock tubes 0 5 1 5 2 mL Vial plate for 15 x 6 mL vials Order online at www agilent com chem store Unit 120 pk 10 pk 90 pk 90 pk 25 pk 30 pk 1 ea 1000 pk 1000 pk 6 pk Part No 0042 1385 0042 1386 0042 6454 0042 1389 0042 8502 0042 1388 0065 4402 0188 5321 0188 5322 G2255 687 00 0022 6538 0022 6539 26 G2258 87102 9065 9948 9065 9950 G2258A 1100 1200 Series Dual Loop Autosampler Description Needle assembly dual loop autosampler Well plate tray 2 well plates 10 vials supp
32. 25 um 50 cm Fused silica PEEK capillary 50 um 50 cm Fused silica PEEK capillary 100 um 50 cm Waste tube PIFE 20 cm 1 4 mm ID 2 0 mm OD MALDI plate carrier Bruker MALDI plate carrier Bruker PAC MALDI plate carrier ABI MALDI plate carrier ABI Opti TOF MALDI plate carrier Agilent MALDI plate carrier Micromass Target Plate for AP MALDI LC MS Calibration Plate Bruker Calibration Plate ABI 192 Calibration Plate ABI 10x10 amp 20x20 Calibration Plate Agilent Calibration Plate Micromass On line matrix Kit for MALDI spotting Includes BCD board cable syringe needles adapters connector capillary Adapter female to female 1 4 28 Adapter male luer to female 1 4 28 Syringe glass 1 mL 1 4 28 connector Micro T connector PEEK swept vol 29 nL with 1 32 in ID fittings MALDI Spotting tips PTFE 10 pk Part No 61364 83205 61364 60021 G1364 27107 61364 84511 61364 87304 61364 87305 61364 87306 61364 86711 0022 6541 0022 6546 0022 6542 0023 0238 0022 6543 0022 6544 G1972 60025 0023 0208 0023 0209 0023 0213 0023 0214 0023 0215 G1364 68706 0042 8517 0042 8518 0181 1541 0042 8519 G1364 81701 Pump Maintenance Regular pump maintenance will help you lower your operating costs and generate precise results you can feel confident about You can count on Agilent isocratic binary quaternary capillary and preparative pumps for superior flow and composition stability And by following a regu
33. 3 0101 1232 0101 1231 0065 9922 3 251 stainless steel prep valve With position sensing 10 mL loop 25 mL syringe ring mounting bracket start cable SS connecting capillaries 0 5 mm PEEK adapter 1 8 in to 1 16 in 4 pk ID 40 cm and 60 cm 5067 1503 Order online at www agilent com chem store 5 Injection Valve Replacement Parts Description Rotor Seals Vespel operating pH 0 to 10 7125 Tefzel operating pH 0 to 14 7125 PEEK operating pH 0 to 14 7125 7725 9725 Rhebuild kit for 7725 Series PEEK operating pH 0 to 14 3725 Rotor seal PEEK for 010 7000 7040 Stator Face Assembly Rheodyne Series 125 Rheodyne Series 25 Stators Rheodyne Series 25 Stators and Seals R Accessories Isolation Seal Rheodyne Series 7725 9725 3 25 Rheotool socket wrench 1 4 in Hex key 9 64 in 15 cm long T handle Ring stand mounting bracket PEEK adapter 1 8 in to 1 16 in 4 pk Position sensor switch for manual valves 1400 3166 Y 6 Order online at www agilent com chem store Part No 0101 0623 0101 0620 0101 1255 0101 1254 0101 1233 0101 1256 0101 0624 0100 1859 0100 1860 1935 4046 8710 2391 8710 2394 1400 3166 0067 1503 0490 1849 Stainless steel sample loops Stainless Steel Sample Loops Remember size designations for loops are nominal The actual volumes can differ from their theoretical designations because of the 0 025 mm 0 001 in tol
34. 5 mm over the needle seat The needle should be replaced when it becomes bent burred or blunt The needle seat should be replaced when it is leaking or plugged You should suspect a leak If you notice a trail of buffer crystals on the needle seat The needle seat can become blocked if the sample contains particulates as this is the first restriction that the sample experiences If this occurs try backflushing the needle seat capillary Tools 1 4 Wrench 2 5 mm Hexagonal key When installing the new needle take care not to strip the setscrew You may have to bend the needle slightly to ensure needle seat alignment Order online at www agilent com chem store Exchanging the Needle lji o Nm INGE E VX y 1 V J K N TuS 1100 1200 Needles and Needle Seats Select Needle Down button until the needle screw shows up in the hole of the safety cover Remove the sample loop Loosen and remove the needle s set screw Move the needle arm to the lowest point using the Needle Down function Install the new needle Align the needle above the needle seat Reconnect the sample loop Situate the needle approximately 2 mm above the seat using the Needle Up function Ensure the needle is aligned with the seat Exchanging the Needle Seat See manual for Well Plate Autosampler Before beginning this procedure e Select Change Needle under
35. 500 80106 G4200 80108 29 0188 6486 HTS and HTC PAL Liquid Injection Valve Supplies HTS and HTC PAL Liquid Injection Valve Loops and Needle Seals Whether your HTS or HTC PAL Description Part No Description autosampler uses the NEW Agilent proprietary Rheodyne high pressure 600 bar injection valve or Valco Cheminert injection valves Agilent can help you find 5 uL 5188 6486 50 uL a sample loop to meet your most Rheodyne 600 bar Valve Supplies Stainless Steel Loops Supplied with Swagelok fittings required for high pressure ee 10 uL 0188 6487 100 uL challenging applications including a 20 pL 5188 6488 e Rheodyne 600 bar injection valve loops which include high pressure Stator Rheodyne 600 bar Swagelok fittings RheBuild Kit 600 bar includes Rotor Seal and 3 32 hex wrench e Cheminert metal loops which feature Needle Seals Rheodyne valve 600 bar 10 pk two 1 16 in stainless steel nuts and two stainless steel ferrules Cheminert Valve Supplies e Cheminert PEEK loops which include PEEK Loops two PEEK nuts and two PEEK ferrules Du 5188 6469 10 pl o IL 0188 6470 20 uL Part No 0188 6489 0188 6490 0188 6491 0188 6492 0188 6478 0188 6467 0188 6468 Metal Loops Include two 1 16 in stainless steel nuts and two stainless steel ferrules 2 uL 0188 6457 900 uL 10 uL 0188 6458 1000 uL 00 pL 0188 6460 2000 pL 100 uL 0188 6461 0000 pL 250 uL 0188 6462 PEEK Needle Seal Valco 22 gauge 10 p
36. aced between the tapered edges of the inserts in such a way that the solvent is evenly distributed over the whole surface of the frit This provides efficient filtration and extends the life of the frit 5084 8273 Low volume Column Inlet Filter A high capacity filter with built in efficiency This low volume column inlet filter is positioned immediately before the LC column so it can remove particles from both the injection system and the sample With a frit diameter of only 2 1 mm plus tapered inserts this filter minimizes external bandspreading while maximizing the filtration capacity 9022 2165 54 Order online at www agilent com chem store 01090 68702 HPLC In Line Filters Description Frit Porosity Frit Inlet ID Flow Rate Part No Replacement Frits um mm RRLC In line filter 0 2 46 max 600 bar 0067 1553 5067 1562 10 pk 4 6 mm 0 2 um pore size filter connecting capillary max 600 bar RRLC In line filter 0 2 ZA max 600 bar 0067 1551 0067 1555 10 pk 2 1 mm 0 2 um pore size filter connecting capillary max 600 bar Low dispersion in line filter 2 1 6 lt 1 ml min 01090 68702 280959 904 10 pk Includes two frits 1 6 mm 2 um 0 5 280959 907 10 pk pore size filter holder with inserts 60 x 0 12 mm connecting capillary Universal in line filter 2 4 8 1 5 mL min 01090 68703 01090 27609 2 pk Includes two frits 4 8 mm 2 um pore size filter holder with inserts 130 x 0 25 mm connecting capillary
37. d rubber septa 100 pk 5181 1216 Screw caps V Z T Z Red aluminum clear PIFE red rubber septa 100 pk 5181 1217 Snap Caps with 11 mm Septa Clear polyethylene clear PTFE red silicone rubber septa 100 pk 5182 0550 Blue polyethylene clear PTFE red rubber septa 100 pk 5182 3458 Green polyethylene clear PIFE red rubber septa 100 pk 5182 3457 Red polyethylene clear PIFE red rubber septa 100 pk 5182 3459 NARE Vial Kits Description Part No 2 mL crimp top vial kit 01078 68705 Includes 100 vials 100 crimp caps 11 mm crimper 2 mL crimp top vial kit 0181 3400 Includes clear crimp top vials silver aluminum crimp caps PTFE red rubber septa in 6 drawer storage box 2 mL amber crimp top vial kit 0181 8801 Includes amber crimp top vials with write on spot silver aluminum crimp caps PTFE red rubber septa in 6 drawer storage box 94 Order online at www agilent com chem store General Supplies Electronic Crimpers and Decappers Whenever large amounts of crimp vials need to be crimped or decapped prene men b ci e the electronic crimper or decapper is the right tool It reduces stress and repetitive E motion injury associated with manual plier style crimpers and decappers Agilent s la m newly designed crimper offers easy hand held pushbutton operation and provides d the following advantages e Stronger and sturdier crimping and decapping e Shorter recharging time and a larger number of battery charges e Better c
38. eedle needle seat peristaltic pump cartridge and seal tight nut Needle assembly well plate autosampler green Needle assembly well plate autosampler new for SN greater than DE21001534 Seat assembly 0 17 mm ID 2 3 pL Seat assembly 0 12 mm ID 1 2 uL Seat assembly for G1367C well plate autosampler without capillary Seat capillary 0 17 x 100 mm 0 8 mm OD use with G136 7 87104 seat Seat capillary 0 12 x 100 mm 0 8 mm OD use with G136 7 87104 seat Stainless steel capillary 210 x 0 12 mm male to male pre swaged Stainless steel capillary 250 x 0 17 mm male to male pre swaged Stainless steel capillary 250 x 0 12 mm 1 fitting Loop capillary 100 uL Peristaltic pump Well plate tray 2 well plates 10 vials supports 50 mm plates Order online at www agilent com chem store Part No G1367 68705 61367 68730 61367 87200 G1367 87201 G1367 87101 G1367 87102 G1367 87104 G1367 87302 G1367 87303 G1316 87328 61367 87304 G1373 87300 61367 87300 0065 4445 G2258 60011 23 24 Micro well plates Order online at www agilent com chem store G1377A 1100 1200 Series Micro Well Plate Autosampler Description Micro well plate sampler accessories kit Includes 100 pk 2 mL screw top vials amp caps 10 pk 0 5 mL 96 well plates tools 40 uL loop 50 cm 50 um fused silica PEEK capillary 75 um seat capillary CAN cable Seat Capillary 50 um 150 mm Fused silica PEEK with fittings Seat Capillary 100 um
39. elution time In addition if the pump keeps working while the filters are blocked the pump could draw in air instead of solvent This will result in periodic baseline disturbances To avoid these problems we recommend these precautions e Use sterile solvent bottles if possible e Filter solvents through sterile filters lt 1pm e Replace solvents every two days e Avoid exposure to direct sunlight or use brown glass bottles 50 Order online at www agilent com chem store Solvent Filters Degassers An added benefit of filtering solvents is that they are degassed at the same time This is particularly beneficial if you do not have an on line degasser in your system The benefits of solvent filtration Degasses eluents as particulates are removed Prevents the formation of spurious peaks within the detector due to solvent outgassing at the low pressure end of the chromatograph Increases solvent inlet lifetime Eliminates pump downtime caused by air locks and particulates in check valves Decreases piston wear while increasing column life Glass solvent filter degasser 3150 0577 Solvent Filters Degassers Description HPLC Solvent filter degasser assembly Replacement Parts for 3150 0577 Glass funnel 250 mL PTFE coated sieve PTFE seal Funnel base glass Filter Membranes Regenerated Cellulose filter membranes 4 7 45 100 pk Nylon filter membranes 4 7 45 100 pk PTFE filter membranes 47 45
40. er G2260A 1100 1200 Series Preparative Autosampler Description Accessory kit Prep autosampler G2260A Includes stainless steel connecting capillaries hex keys wrenches 100 pk 2 mL screw cap vials and caps tray for 6 mL vials stainless steel union and other parts MBB Injection valve for G2260A sampler PEEK rotor seal and stator face Stator head for 0101 1267 valve Needle seat prep autosampler 0 5 mm ID 20 uL Needle assembly for G2260 87101 needle seat Capillary injection valve to preparative head SS 16 cm 0 5 mm Capillary sampler to column SS 60 cm 0 5 mm Capillary pump to sampler SS 40 cm 0 5 mm Multi draw loop for use with G2260A recommended for injection volumes up to 5 mL Order online at www agilent com chem store Part No G2260 68705 0101 1267 0101 1268 0100 2195 G2260 87101 G2260 87201 G2258 87301 G2260 87300 G2260 87301 G2260 68711 2 HTS and HTC PAL Liquid Injection Vials and Caps HTS and HTC PAL high throughput LC injection systems are configured to cope with today s high throughput LC MS demands And Agilent brings you a wide choice of well plates vials and caps that are engineered to work with these injectors providing the flexibility you need HTC PAL autosampler 28 Order online at www agilent com chem store HTS and HTC PAL Liquid Injection Vials and Caps Description 2 mL vials Screw top vial wide opening clear Screw top vial polypropylene w
41. er blend and feature a spring made from the same high quality stainless steel that is used in our pump s flow path The combination of our piston and seal has undergone extensive testing under temperature stress with all common HPLC solvents and in many instruments More importantly they yield consistent reproducible results Order online at www agilent com chem store 41 Changing the Pump Seals Pump seals should be replaced when there are leaks on the bottom of the pump head when retention times are inconsistent or when the pressure ripple is unstable 42 Order online at www agilent com chem store All pumps except the G1361A preparative pump 1 Disassemble the pump head and use a piston to remove the two seals NO Clean the pump chambers and seal areas by flushing with solvent and wiping with a lint free cloth Co Insert new seals s Reassemble the pump head O1 Perform the seal wear in procedure Standard seals 5063 6589 only The wear in procedure involves flushing the seals with isopropanol at high pressure while they are in place Changing and Inspecting the Sapphire Pistons All pumps except the G1361A preparative pump Each time you replace the seals examine Disassemble the pump head assembly the pistons for scratches which can cause small but damaging leaks NO Remove any deposits particularly buffers from the piston surface usi
42. erance of the metal tubing bore Generally accuracy rates are about 5 for large metal loops 1 0 mm 0 040 in 10 for intermediate loops 0 5 mm 0 020 in and 30 for small loops 0 2 mm 0 007 in However since both standards and unknowns are typically analyzed using the same sample loop knowledge of the actual volume is rarely needed If you do need to know the sample volume it is best to calibrate the loop while it is on the valve so the valve s flow passage is also taken into account Sample Loops Stainless steel loop ends are square cut and burr free for a flush connection to the valve They are also supplied with unswaged fittings Flexible PEEK loop ends feature a clean straight cut for low dead volume connection Both types of sample loops are factory cut and finished to the highest quality Stainless Steel Loops for 7125 and 7010 Injectors Description Opl 10 uL 20 pL JO uL 100 uL 200 uL 500 uL 1 mL 2 mL o ML ID mm Part No 0 18 1535 4860 0 30 0101 0376 0 51 0101 0377 0 51 0101 0378 0 51 0101 0379 0 76 0101 1252 0 76 0101 1251 0 76 0101 1219 1 00 0101 1250 1 00 0101 1249 Order online at www agilent com chem store 7 Stainless Steel Loops for 7725 Injectors Description ID mm Part No b pL 0 18 0101 1248 10 uL 0 30 0100 1923 20 uL 0 30 0100 1922 50 uL 0 51 0100 1924 200 uL 0 76 0101 1247 500 uL 0 76 0101 1246 1 mL 0 76 0101 1245 2 mL 1 00 0101 1244 9 mL 1 00 0101
43. ernal tray or funnel tray or contaminated Order online at www agilent com chem store Trays collecting tubes and plates for 1100 1200 Series Fraction Collectors Agilent offers a broad range of trays glass collecting tubes and well plates When deciding what s best for your application consider the size and number of the fractions you want to collect Well plate tray Collecting Tubes and Trays Tray Part No 61364 84523 G1364 84524 61364 84525 G1364 84516 G1364 84532 Well Plate Trays Description Hole Diameter mm 30 29 16 12 Funnel tray Tray for 4 well plates cooled No of Tubes 40 60 126 215 40 Tray for 4 plates adjustable cooled Tray for 2 well plates 10 funnels cooled Tray for 2 well plates 10 vials 2 mL Fraction Collector Maintenance Tube Dimensions 30 x 48 mm 20 x 100 mm 16 x 48 mm 12 x 48 mm Any size Order online at www agilent com chem store oa Ne Tube Unit Part No 5042 6470 100 pk 5042 6459 100 pk 5022 6533 100 pk 5022 6534 100 pk Part No 61364 84521 61364 84531 61364 84522 G1367 60001 33 9022 6538 Well Plates Description 96 Well plates 0 5 mL polypropylene 96 Well plates 0 5 mL polypropylene 96 Deep well plates 1 mL polypropylene Closing mats for 96 well plates silicone 96 Well Plates 150 uL conical polypropylene 384 Well plates 90 uL polypropylene 384 Well plates max 45 uL skirted 96 Deep well plate
44. finger caps G1313 27A Autosampler accessory kit G1313 68705 Includes 3 hex keys 2 wrenches tubing 1 label halftray wrist strap 15 finger caps 0 17 ID x 180 mm capillary 100 screw top 2 mL vials and caps tray for 40 2 mL vials tray for 15 6 mL vials G1313 27A Autosampler Preventative Maintenance Kit 0065 4498 Includes 1 each rotor seal isolation seal stator face needle needle seat PM Kit for standard autosamplers G1313 68730 Includes Vespel rotor seal standard needle seat and needle Light protection kit for G1329A G1329 68718 Includes opaque front and side doors and front cover Door replacement kit for G1329A G1329 68727 Includes transparent front and side doors Order online at www agilent com chem store 19 20 Sapphire piston and seals 1100 1200 Metering Device Supplies Infreguently the metering device seal and piston may need replacement if you see loss in injection volume precision or metering device leaking Order online at www agilent com chem store 1100 1200 Metering Device Supplies Description Sapphire Piston Piston Seals graphite filled Teflon reversed phase 2 pk Metering valve seal 900 uL Sapphire piston assembly 900 uL Capillary to metering device Multidraw upgrade kit for G1313A G1327A G1329A autosamplers Includes 500 uL capillary 1500 uL capillary and ZDV union Seat capillary 500 uL 0 5 mm ID Seat capillary 1500 uL 0 9 mm ID 1 16 in union zero dead volume stainles
45. hanger MS DAD cell TCC IGG Cooler 50 150 mm column Cooler 20 30 mm column Detector DAD cell Order online at www agilent com chem store 1200 Rapid Resolution Connection Capillaries ID mm 0 17 0 17 0 17 0 17 0 12 0 17 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 12 0 17 0 12 0 12 0 12 0 12 0 17 0 17 0 12 0 12 Length mm 400 700 150 105 000 310 310 100 130 340 79 000 50 70 90 130 150 170 210 300 340 105 170 o 1 0 Connection Male to male Male to male Male to male Male to male Male to male Male to male Male to male Male to male Male to female Male to male Male to male Male to male Male to female Male to female Male to female Male to female Male to female Male to male Male to male Male to male Male to male Male to male Male to male Male to female Male to female Fittings Pre swaged Pre swaged Pre swaged Pre swaged 1 end pre swaged 1 end pre swaged 1 end pre swaged Non swaged Non swaged 1 end pre swaged Non swaged 1 end pre swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Non swaged Part No G1312 87303 G1312 87304 G1312 87305 G1312 87306 G1315 8 30 G1315 87319 G1315 87339 G1316 2 301 G1316 87304 G1316 8 305 G1316 87306 G1316 87309 G1316 87312 G1316 87313 G1316 8 314 G1316 8 315
46. hic performance with these tips and techniques Agilent wavelength detectors combine exceptional flexibility with superior instrument control data communication and analytical capabilities And this section will show you how to maintain your detector s high level of selectivity and sensitivity by performing the following procedures e Choosing the most compatible flow cell for your application e Disassembling cleaning and installing the flow cell e Changing the deuterium lamp Of course you will also find the precision engineered Agilent parts you need to get the job done quickly and keep your downtime to a minimum Order online at www agilent com chem store 57 Variable Wavelength Detector It is critical to verify the function of the VWD on a regular basis There are two built in tests to help you accomplish this e The wavelength calibration test e The lamp intensity test If the lamp intensity has diminished more than Is tolerable for your application you will need to replace the lamp Additionally you should perform the following routine maintenance procedures e Check the waste outlet e Clean the leak sensor e Check the photocurrent e Clean or replace the flow cell windows when they become dirty Replacement Parts Description Part No G1314A VW detector accessory kit G1314 68705 Includes 1 4 in waste tubing 2 hex keys 2 wrenches outlet tubing 1 16 in PEEK male fitting Deuterium lamp 10
47. ise Pump mounted on cushioned grommets to minimize vibration 2 Integrated fans maintain temperature inside cover LEDs and audible alarm if temperature exceeds 35 C limit Maximum ambient temperature of 35 when airflow Is neither restricted nor recycled Standard one year warranty Installation and familiarization included with new LC MS orders The Quiet Cover Il is compatible with these Agilent LC MS systems 6100 Single Quads G6110AA G6120AA G6130AA G6140AA 6300 Traps G2440DA G2451AA G4533AA 624 435 6410 QQQ G6410AA 6210 TOF G3250AA G3252A 69104A Q TOF G6510AA Or any analytical system using BOC Edwards pumps lbs kg E2M28 E2M18 E1M18 Description Part No Quiet Cover II for Agilent LC MS Systems G3199B Zo Wx lo Hxo3 5L General Supplies They may be small but they have a BIG effect on your productivity and results Vials caps and other accessories are the least expensive components of your LC system however they can contribute to problems such as injector damage ghost peaks and analyte degradation That is why Agilent supplies are engineered with the same reliability and reproducibility you expect from Agilent instruments And they are precisely designed to work with your 1100 or 1200 Series HPLC System The following pages feature a wide selection of Agilent engineered supplies including e Membrane syringe filters e LC tubing e Connecting capillaries e Certified vials and caps
48. ith 0 2 mL integrated glass insert Crimp top vial wide opening clear Crimp snap top vial clear write on spot 2 mL caps Screw cap PIFE silicone septa Screw cap pre slit PIFE silicone septa Crimp cap silver aluminum PTFE silicone septa Snap cap blue polyethylene PIFE silicone septa Micro vials Crimp top vial 0 8 mL amber glass flat bottom Crimp top vial 0 1 mL clear tapered Crimp top vial 0 3 mL clear round Crimp top vial 0 7 mL amber round Crimp top vial 0 5 mL amber conical Micro caps Crimp caps with PTFE silicone septa Unit 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 100 pk 1000 pk 900 pk 900 pk 900 pk 900 pk 900 pk Autosampler Supplies for Agilent HPLC Systems with CTC Autosamplers Agilent now offers a portfolio of CI C recommended supplies for your HTS and HTC PAL autosamplers This complete selection of vials caps syringes and valves meets Agilent s stringent standards and specifications Part No 5182 0714 0188 5390 0181 3375 0182 0546 0182 0720 0183 2076 0182 0552 0182 0541 0183 4487 0180 0844 0180 0841 0180 0805 0180 0806 0180 0842 HTS and HTC PAL Liquid Injection Syringes Designed specifically for CTC autosamplers these X type syringes feature zero carryover and a long lasting plunger for applications that demand precision and productivity They are strongly recommended for high throughput applications Agilent offers
49. k Teflon Needle Seal Valco 22 gauge 10 pk 30 Order online at www agilent com chem store 0188 6463 0188 6464 0188 6465 0188 6466 0188 6476 0188 6477 Fraction Collector Maintenance A higher level of purity tor results that stand up to scrutiny When you are under pressure to generate better results faster there is no time to compromise on recovery and purity And with Agilent s 1100 and 1200 Series fraction collectors you will not have to Agilent fraction collectors are designed to process data in real time for instantaneous and precise fraction collection while increasing throughput on your purification system So you can be certain you are getting the highest degree of recovery and purity for your fractions even with low flow rates In this section you will find essential details about our analytical scale AS and preparative scale PS fraction collectors including e Fraction collector maintenance including simple repairs you can perform without a service call e Guidelines for maintaining the micro fraction collector and spotter e Agilent engineered parts such as trays collecting tubes plates and needle assemblies e MALDI spotting accessories Order online at www agilent com chem store 31 Fraction collector Tubing Replacement There are two types of tubing available to connect the valve with the needle preparative scale PTFE tubing 0 8 mm ID and analytical scale tubing
50. k architecture that allows you to switch between LOAD and INJECT positions without interrupting the flow So you can analyze more samples in less time In this section you will find the latest in LC injection valve technology along with important maintenance information that will maximize reproducibility Order online at www agilent com chem store 3 Agilent Injection Valves from Rheodyne Patented Make Before Break MBB technology that provides uninterrupted flow when switching between LOAD and INJECT positions Additionally MBB greatly reduces transient pressure shocks which benefits flow sensitive detectors and extends column life Dual mode or front loading injection which enables either the partial filling or the complete filling of the sample loop An injection port built right into the handle This unique design allows the tip of the needle to connect directly to the sample loop So you don t have to worry about sample loss during loading The reproducibility of manual sample injectors depends on operator skill syringe precision and loading method The partial filling method is typically reproducible to 1 0 relative standard deviation RSD The complete filling method is reproducible to 0 1 RSD for 5 uL loops Series 77251 and 97251 Analytical Injection Valves Stainless steel SS 77251 and PEEK 97251 valves are the most popular injection valves for analytical HPLC Features include e A 20 ul loop in
51. laintaining Your Agilent LC and LC MS Systems i Table of Contents Manual Injection Valve Maintenance 3 Agilent Injection Valves from Rheodyne Ssiccsciccsecasenssnssnsnspuainssaseasanssinuosaiiesaenshaaeisleunsans giadondiionsbweidevstaniboaieneustanunasounenes 4 gampile LOODS A A A A O ER EARE PAEST 7 Syringes for Manual Injection VaIV ES asane ae de vo oda a zdelo alenka 10 CDOT el oo UE O NO ee 11 Autosampler Maintenance 13 lese y we Ms za ee o A m eo oeeannknnnannmnanmm 14 POOF ZUJE SE and NE OE a E a a Ned 16 Autosampler Parts and SUPPIIGS tacts A a a a A MM NE 19 Autosampler Supplies for Agilent HPLC Systems with CTC Autosamplers 28 Fraction Collector Maintenance o Analytical and Preparative Fraction Collector Maintenance 32 Micro Fraction Collector Spotter Maintenance 35 Pump Maintenance 3 s a a nn of Pistons and ee Satan A A hc aac A NA RE A leven decor NE AN 41 n ApoE aana E E E AA E R TREE 46 O eee E E T AE eee 50 NE Ne a aa Ne b4 MMU Bele es po A AAEN A A AT A E N NA ME TA VENO LE 56 Detector Maintenance s Variable Wavelength EO a Ne navi pol a senata s 58 Diode Array Detector DAD Multiple Wavelength Detector MW 64 Maintaining th INO a la noga 12 C OVO e E E E E E RAE E AAA nana 76 General upplleS ae 11 Ready to Use Econofilter Membrane Syringe Filters ena 78 WE WG OVA NIM E a a 19 Eo ea
52. lar maintenance routine you can also count on maximum uptime and a steady accurate solvent flow for the life of the pump On the following pages you will find step by step instructions for these procedures e Exchanging the PTFE frit e Cleaning the outlet ball valve e Replacing the active inlet valve cartridge e Changing the seals and pistons e Cleaning or replacing the solvent inlet filters You may perform these procedures all at once or as needed Keep in mind that some parts may need replacing more often than others depending on your application and solvent preparation procedures Order online at www agilent com chem store 37 38 Purge valves feature a gold seal held in place with a plastic cap Small leaks can occur here so you will need to replace the gold seal after the valves have been removed more than once or twice When replacing the frit make sure that the cutout side faces up which provides more surface area on the side facing the solvent flow 1 Remove capillary and waste tube 2 Use a 14 mm wrench to unscrew the valve 3 Remove the plastic cap and the gold seal 4 Use tweezers to remove the old frit and install a new frit 5 Replace the gold seal and the plastic cap 6 Install the valve Purge Valve Exchanging the PTFE Frit 1100 1200 standard pump modules Purge valve assembly Purge Valve Description 1100 Pump Start Up Kit Includes 1 outlet cap 2 PIFE fri
53. laries 2 Switch on the pump and check the cell for leaks Once you are convinced the cell is leak free 3 Install the flow cell into the instrument 4 Screw down the cell We recommend the following tests after cell installation e Zero order calibration e Wavelength calibration Because the flow cell is part of the optical system and has now been moved since these tests were last performed we recommend running them again before your next analysis There are two designs of the Standard Flow Cell The cell screw of the new design G1314 60086 Is painted black G1314 60086 Standard Flow Cell Replacement Parts Item Description Unit Part No Standard D type flow cell 10 mm G1314 60086 14 uL 40 bar Standard D flow cell kit G1314 65061 Includes 2 windows 2 gaskets 1 2 gaskets 2 1 Cell screws 2 pk 1314 65062 2 Conical spring D version 10 pk 79853 29100 3 Ring 1 PEEK 2 pk G1314 65065 4 Gaskets 1 Kapton 10 pk 61314 65063 D Quartz windows D version 2 pk 79853 68742 6 Gaskets 2 Kapton 10 pk 61314 65064 7 Ring 2 PEEK 2 pk G1314 65066 The cell screw of P N G1314 60086 is painted black to differentiate flow cell types Order online at www agilent com chem store 61 62 Order online at www agilent com chem store G1314 60080 Standard Flow Cell Replacement Parts old design Item Description Oo O17 Wy N G1314 60083 Semi micro Flow Cell Standard D flow cell ki
54. laries 0 17 mm ID Sample enrichment Capillaries 0 17 mm ID Solvent selection Flow rate up to 10 mL min Valve Kit G1157A G1157A G1316A 057 G1159A G1316A 055 G1158A G1160A Order online at www agilent com chem store Part No G1156 68711 G1156 68713 G1316 68711 G1156 68712 G1316 68710 G1156 68714 G1160 68706 11 12 Switching valve Switching Valve Replacement Parts Use With G1160A G1159A G115 A G1158A G1158B G1162A G1163A Order online at www agilent com chem store Description 12 Position 13 Port Preparative Solvent Selection Valve 6 Position 14 Port Column Selection Valve Six Column Selector 2 Position 10 Port Valve Dual sided MBB 2 Position 6 Port Switching Valve 2 Position 6 Port 600 bar Switching Valve 2 Position 6 Port Micro Switching Valve 2 Position 10 Port Micro Switching Valve RheBuild Kit Part No 0101 1288 0101 1290 0101 1359 0101 1358 PEEK Rotor Seal Part No 0100 1855 Vespel 0100 1854 Tefzel 0100 2233 PEEK 0101 1409 HP PEEK blend 0100 2087 0101 1361 Stator Part No 0101 1365 0101 1364 0101 1362 Stator head 0100 1850 Stator head 0101 1417 0100 2089 0101 1363 i Hirer tl aAa Autosampler Maintenance Ensuring peak performance productivity and peace of mind Your Agilent autosampler is designed to deliver accurate measurement
55. learance and more flexibility thanks to improved crimp jaws e individual test certificates Electronic Crimpers and Decappers Electronic crimper Description Part No 11 mm Electronic Crimper with 4 8v rechargeable battery pack 0062 0207 20 mm Electronic Crimper with 4 8v rechargeable battery pack and charger 0062 0208 11 mm Electronic Decapper with 4 8v rechargeable battery pack and charger 9062 0209 20 mm Electronic Decapper with 4 8v rechargeable battery pack and charger 0062 0210 4 8v nickel metal hydride replacement battery 0188 6565 Order online at www agilent com chem store 95 96 Add the power of remote monitoring and instrument diagnostics to industry leading service and support agreements with Agilent Intelligent Services Based on a secure internet connection between your instruments and Agilent s customer support centers Intelligent Services offers you the benefits of proactive technical support and remote interactive collaboration to help you maximize system uptime and optimize lab productivity Agilent Intelligent Services Get real time help with a few clicks of your mouse When one of your instruments unexpectedly needs service you want to reach someone who can help you right away With Intelligent Services Push for Help you ll skip the wait in call center queues and be connected to the expert support you need without delay You simply click on the Push for Help icon on your PC screen Im
56. let filters at least once every 3 months Inlet Filters Description Glass filter solvent inlet 20 um pore size Glass filter solvent inlet 40 um pore size Glass filter solvent inlet 40 um pore size ers reducing Part No 0041 2168 3150 0944 3150 0944 Glass filter Frit Adapter Description Frit adapter PTFE 3 mm 4 pk Frit adapter 4mm PIFE G1361 23204 Frit adapter PTFE for 1 8 in OD tubing Part No 0062 8517 G2258 23201 Filter frit adapters 5062 851 7 Frit Inlet ID mm Tube OD mm Recommended Use D 7 7 32 Analytical scale micro scale 4 Preparative LC 3 2 G2258A Dual Loop autosampler Order online at www agilent com chem store 53 HPLC In Line Filters Column inlet frit contamination can increase column back pressure and reduce efficiency Microbore column blockages are a particular problem due to the small diameter of the inlet frit lo prevent blockages always use the appropriate filters in your LC system Agilent offers two types of high pressure in line filter kits for use with any HPLC system Universal Solvent Filter Ideal for microbore narrow bore high speed or standard analytical columns Universal solvent filters are installed between the LC pump and the injector so particles from the solvent can be removed before they reach the injector The filter assembly consists of a 4 8 mm frit two inserts and a two piece holder The frit is pl
57. llows 1 Use a 14 mm wrench to remove the active inlet valve 2 Change the cartridge 3 Reinstall the active inlet valve Replacing the Active Inlet Valve Cartridge All pumps except the G1361A preparative pump Active Inlet Valve AV Active Inlet Valve AIV Description Active inlet valve without cartridge Part No G1312 60025 0062 8562 Cartridge for active inlet valve 400 bar Cartridge for active inlet valve 600 bar Gold Seal for inlet valve 40 Order online at www agilent com chem store G1312 60020 9001 3708 Sapphire piston and seals Pistons and Seals Agilent pistons are made from a high purity monocrystalline sapphire for maximum durability Although ceramic pistons can be manufactured at a lower cost ceramic Is a sintered polycrystalline material which can cause undesirable variations during the production process Agilent sapphire pistons e Are meticulously cut at just the right angle making them the most durable and longest lasting pistons in the world e Are precisely aligned in their stainless steel holder to minimize wear on the piston and seal Agilent seals are designed to fit snugly around our pistons and are capable of adapting to a wide range of flow rates and pressures Agilent piston seals e Are spring loaded and engineered to deliver optimal performance over highly dynamic flow and pressure ranges e Are manufactured from a proprietary polym
58. ludes items 1 through 5 Window screw Spring washers 10 pk Compression washer Window holder Quartz window Seal BACK 12 pk Seal FRONT 12 pk Inlet capillary with heat exchanger 0 12 mm ID 290 mm long for G1315 60011 Outlet capillary 0 12 mm ID 200 mm long Outlet capillary 0 17 mm ID 200 mm long Clamp unit Screw M 2 5 4 mm long for cell body clamp Cell repair kit semi micro Includes window screw kit 4 mm hexagonal wrench and seal kits Part No G1315 60011 G1315 60025 G1315 60024 79883 68703 79883 22402 0062 8553 79883 28801 79883 22301 1000 0488 79883 68702 61315 68710 61315 87325 G1315 87306 G1315 87302 G1315 84901 0515 1056 G1315 68713 G1315 60015 Micro High Pressure Flow Cell and Replacement Parts Item Description S I O O17 S WY N Micro high pressure flow cell 6 mm 1 7 uL 400 bar Window assembly includes items 2 through 6 Seal ring Ouartz window high pressure Compression washer high pressure Spring washers 10 pk Window screw Inlet capillary with heat exchanger 0 12 mm ID 290 mm long for G1315 60011 Outlet capillary 0 12 mm ID 200 mm long Clamp unit Screw M 2 5 4 mm long for cell body clamp Column connecting capillary with fittings 150 x 0 12 mm Column connecting capillary with fittings 380 x 0 17 mm High pressure cell repair kit Includes 1 quartz window 5 spring washers 2 seal rings Order online at w
59. mediately an Agilent Service Professional receives all the information needed to begin working on your problem over a secure Internet connection Then the Agilent Service Professional calls you to offer a solution Solve problems fast without waiting for an onsite visit Did you know that it s possible for Agilent experts to diagnose and solve problems remotely With your permission we can use Real Time Collaboration to perform interactive system diagnostics and provide you with the fastest fix possible Asset reports provide the knowledge that ensures greater productivity Imagine how easy it would be to plan your laboratory operations if you had an inventory of your Agilent assets that was updated automatically Intelligent Services Asset Report provides this information and much more on demand so that you can use it to increase your laboratory productivity Intelligent Services is now available for 1100 1200 Series LC Systems and Single Quad LC MS Systems with more offerings coming soon For more information visit www agilent com chem intelligentservices Order online at www agilent com chem store 1100 1200 Series LC System Recommended Maintenance Schedule Pumps Item PTFE frits in purge valve Solvent inlet filter Piston seals outlet gold seal plastic cap wash seal gasket wash seal Piston cartridge active inlet valve outlet ball valve Autosampler Item Guard column Piston seal isolation
60. mpatible with 1100 1200 standard and capillary pumps The active seal wash uses a peristaltic pump to flow the wash solvent and is compatible with 1100 pumps with the following serial numbers and higher as well as the 1200 Series pump e G1310A DE40906378 e G1311A DE40926032 e G1312A DE40914884 _ 5042 8507 Seal Wash Description Part No Active seal wash kit for isocratic or quaternary pumps G1311 68711 Includes 2 wash seal gaskets 2 pump seals peristaltic pump includes pump cassette and motor 2 seal keepers 2 support ring assemblies seal insert tool silicone tubing Active seal wash kit for binary pumps G1312 68711 Includes 4 wash seal gaskets 4 pump seals 2 peristaltic pumps includes pump cassette and motor 4 seal keepers 4 support ring assemblies seal insert tool silicone tubing Continuous seal wash kit 01018 68722 Includes 2 wash seal gaskets 4 m flex tubing 2 pump seals 1 flow regulator 2 seal keepers 2 support ring assemblies 20 mL luer lock syringe seal insert tool abrasive paper Silicone Tubing 1 mm ID 3mm OD 5 m Seal Keeper Wash seal Wash seal gasket Peristaltic pump cassette with silicone tubing 0065 9978 0001 3743 0905 1175 0062 2484 0042 8507 Order online at www agilent com chem store 45 Pump Supplies G1311 12 54A 1100 1200 Series Standard Pump Description Pump Start Up Kit Includes 1 outlet cap 2 PIFE frits 4 piston seals 1 outlet gold
61. n Unit Part No 2 mL Wide Opening Screw Top Glass Vials Clear Clear Clear silanized Clear write on spot Clear write on spot Clear write on spot silanized Amber write on spot Amber write on spot Amber write on spot silanized 100 pk 5182 0714 1000 cs 5183 2067 100 pk 5183 2070 100 pk 5182 0715 1000 cs 5183 2068 100 pk 5183 2071 100 pk 5182 0716 1000 cs 5183 2069 100 pk 5183 2072 Order online at www agilent com chem store 91 Vials Description 2 mL Wide Opening Crimp Top Glass Vials Clear Clear Clear silanized Clear write on spot Clear write on spot Clear write on spot silanized Amber Amber Amber write on spot silanized 2 mL Wide Opening Snap Top Glass Vials Crimp top vials and caps Clear Clear Clear silanized Clear write on spot Clear write on spot Clear write on spot silanized Amber write on spot Amber write on spot Amber write on spot silanized Wide Opening Polypropylene Crimp Snap Top Vials Snap top vials and caps Polypropylene 1 mL Polypropylene 300 uL 92 Order online at www agilent com chem store Unit 100 pk 1000 cs 1000 cs 100 pk 1000 cs 100 pk 100 pk 1000 cs 100 pk 100 pk 1000 cs 100 pk 100 pk 1000 cs 100 pk 100 pk 1000 cs 100 pk 100 pk 1000 pk Part No 9181 33 5 0183 4491 0183 4494 0182 0543 0183 4492 0183 4495 0181 3376 0183 4493 0183 4496 0182 0544 0183 4504 0183 4507 0182 0546 0183 45
62. ng alcohol or toothpaste Replace the pistons if scratched COD Rinse thoroughly in alcohol and HPLC grade water ENS Reassemble the pump head O1 Check to make sure there are no fractures in the springs Replace piston housing assembly if springs are damaged OD Reinsert the pistons Seals and Pistons Description Part No Sapphire Piston 0063 6586 Piston Seals graphite filled Teflon reversed phase 2 pk 0063 6589 Piston Seals polyethylene normal phase 2 pk 0905 1420 Piston housing assembly includes springs G1311 60002 Extended PM kit 0065 4499 Contains 5063 6589 piston seals 2 01018 22707 PTFE frits 5 pk 0062 8562 cartridge active inlet valve G1311 60012 outlet ball valve amp 5063 6586 pistons 2 Order online at www agilent com chem store 43 44 Important Do not perform this procedure when installing normal phase seals 0905 1420 It will cause severe damage to the seals Tools e Solvent bottle or beaker e lsopropanol 6 e AIV adapter 0100 1847 e Restriction capillary 0022 2159 i Seal Wear In Procedure Recommended after replacing standard seals only 5063 6589 Fill the bottle with 100 mL of isopropanol and place it in the solvent cabinet Then place the new pump head s tubing including bottle head assembly into the bottle screw the adapter to the Active Inlet Valve Connect the inlet tube from the bottle head directly to the adapte
63. or dip tube G1946 80054 Cloths lint free 05980 60051 Cotton swabs 100 pk 0080 5400 Gas Purifiers and Generators Big hydrocarbon trap 1 4 in fittings BHI 4 Big universal trap 1 8 in fittings nitrogen RMSN 2 Nitrogen gas generator 0183 2003 Nitrogen gas generator with oxygen monitor 120 220V 0183 2004 Maintenance kit for LC MSD nitrogen generator 0183 2014 Pump Supplies Oil mist filter kit for E2M18 3162 1056 Oil return kit 3162 1057 Rotary pump oil 4 L 6040 0798 Rotary pump oil 1 L 6040 0834 Tools G1946A tool kit G1946 60157 3 mm wrench for nebulizer needle adjustment 8710 2699 Nebulizer adjustment fixture G1946 20215 Nebulizer 25X magnifier G1946 80049 Plastic tubing cutter 8710 1930 These parts are common to all LE MSD systems Order online at www agilent com chem store 75 Quiet Cover 76 Order online at www agilent com chem store Quiet Cover Agilent has a solution to the frequent maintenance and inherent noise of LC MS rough pumps The Quiet Cover was designed for easy movement maintenance and better living with rough pumps used with Agilent and other LC MS systems Locking castors to move heavy pump for maintenance No tools necessary to remove sectioned cover for easy access to pump Built in Lift and Tilt lever raises end of pump to drain oil Removable drip pan with well and hand holds to collect and transport oil sound absorbing cabinet with resistant foam insulation to reduce pump no
64. orts 50 mm plates 1100 Well plate tray 2 well plates 10 vials supports 50 mm plates Twin needle seat dual loop autosampler Front seat tube SS 0 5 mm ID 10 cm long Back seat tube SS 0 5 mm ID 12 cm long Front seat tube PTFE 0 2 mm ID 10 cm long 5 uL Back seat tube PTFE 0 25 mm ID 12 cm long 6 uL Waste tube 0 8 mm ID 15 cm long Waste tube 0 8 mm ID 10 cm long Buffer loop extension assembly Buffer loop tubing assembly PTFE 1 4 mm ID 2 0 mm OD Drawing tube assembly for flush solvent with filter and bottle cap Tubing assembly solvent flush Fitting screws SS 10 32 4 mm 5 pk PEEK ferrule and stainless steel ring for 2 mm tube 5 pk Union PEEK for 1 8 in OD tubing 1 4 28 internal threads 10 Port Dual Loop Valve Rotor seal Vespel 10 port valve Stator DuraLife 10 port valve Vial plate for 15 x 6 mL vials Vial plate for 54 x 2 mL vials 6 pk Frit adapter PIFE for 1 8 in OD tubing Piston for G2258A Dual Loop ALS 5 mL Piston guide PEEK for G2258A ALS Piston seal for G2258A Dual loop ALS Order online at www agilent com chem store Part No G2258 68710 G2258 60001 G2258 60011 G2258 8 7102 G2258 8 316 G2258 8 315 G2258 8 312 G2258 8 313 G2258 87310 G2258 87311 G2258 60002 G2258 87300 G2258 8 307 G2298 8 314 0065 9948 0065 9950 0100 2410 0101 1385 0101 2415 0101 1390 0022 6539 G2255 687 00 G2258 23201 62258 60003 G2258 23101 0905 1599 G2260 preparative autosampl
65. pler Description Unit Part No Clear screw vial 16 mm cap size 100 pk 9301 1377 Screw caps 16 mm 100 pk 9301 1379 PTFE silicone septa 16 mm 100 pk 9301 1378 Clear crimp vial 20 mm cap size 100 pk 9301 1419 Crimp caps PIFE silicone septa 20 mm 100 pk 9301 1425 Clear screw extreme high recovery vials 16 mm cap size 30 pk 5188 2757 PTFE silicone septa pre slit 16 mm 100 pk 5188 2758 Clear 5 mL high recovery screw cap vials 30 pk 5188 5369 G1387A 1100 1200 Series Micro Autosampler 6 mL vials caps and septa Description Part No Capillary sampler accessories kit G1329 68715 2 Position 6 Port Micro Injection Valve 0101 1050 Rotor seal Vespel 2 grooves 0100 2088 Stator 0100 2089 6 fittings 2 plugs PEEK 0065 4410 Sapphire piston 2 mm 0064 8293 Piston seal 2 mm 9022 2175 Seal support assembly G1377 60002 Seat assembly 100 um ID 1 2 uL G1329 8 101 Seat assembly 50 um ID 0 3 uL G1329 87103 Needle assembly micro LC autosampler G1329 80001 Waste tube FEP 1 6 mm OD 0 8 mm ID G13 5 8 326 Stainless steel capillary pump ALS 90 cm 0 17 mm ID G1329 87300 liji Order online at www agilent com chem store G1367A well plate autosampler G1367A B C 1100 1200 Series Well Plate Autosampler Description Well plate sampler accessories kit Includes 100 pk 2 mL screw top vials amp caps 10 pk 0 5 mL 96 well plates tools 380 mm 0 17 mm capillary CAN cable PM Kit for well plate autosamplers includes Vespel rotor seal n
66. r quat pumps and binary pumps with selection valves only Connect the restriction capillary to the purge valve Insert the other end of the capillary into a waste container Open the purge valve and purge the system for 5 minutes with isopropanol at a flow rate of 2 mL min Close the purge valve and set the flow to a rate that is adequate to achieve a pressure of 350 bar Pump for 15 minutes at this pressure to wear in the seals You can monitor the pressure at your analog output signal using the handheld controller ChemStation or any other controlling device connected to your pump Turn OFF the pump flow and slowly open the purge valve to release pressure from the system Disconnect the restriction capillary and reconnect the outlet capillary to the purge valve Reconnect the appropriate tubing to the Active Inlet Valve 8 Rinse your system with the solvent that you plan to use for your next application Order online at www agilent com chem store j Pump Head Seal Wash Kits Used with standard and capillary pump The routine use of highly concentrated buffer solutions 100 mM will reduce the life of the seals and pistons in your 1100 1200 Series pump You can counteract the problem with one of our seal wash kits which flushes the backside of the seal with a wash solvent water isopropanol 90 10 is recommended as the wash solveni The continuous seal wash uses gravity to flow the wash solvent and is co
67. r when contaminated blocked worn or visibly damaged Before replacing the capillary be sure to 1 NO oO oo A Place the transport unit of the micro fraction collector spotter in the Home position Remove all installed trays from the tray base Place the transport unit in the Change Parts position Turn off the instrument Unscrew the micro fraction spotter capillary from the flow cell of the detector x lt _ i 2 aii o o _ Micro fraction collector Simple Repairs Procedure Exchange the capillary guiding assembly Exchange the internal tray Exchange the flap septum and waste tubing Fraction Collector Maintenance I o o When to Perform Replace fraction collector capillary At least every six months or when worn blocked or damaged When bent or damaged When the flow delay sensor no longer works properly At least every six months Or when defective or contaminated Order online at www agilent com chem store 35 36 G1364 83205 61364 60021 0022 6541 9023 0208 Order online at www agilent com chem store G1364D 1100 1200 Micro Fraction Collector Description MALDI spotting adapter for G1364D Micro fraction collector Well plate adapter assembly for G1364C D Flap septum PEEK for internal tray Tray for 4 plates adjustable cooled for use with G1364D Micro fraction collector Fused silica PEEK capillary
68. rder online at www agilent com chem store LC MSD Chemical Supplies Description LC MSD Quad Supplies ESI Tuning Mix 100 mL APCI APPI calibrant solution 100 mL ES Negative lon Performance Standard 5 x 1 mL ampoules Delay Calibrant LC MSD Trap Supplies ESI Tuning Mix for lon Trap 100 mL APCI APPI calibrant solution 100 mL LC MSD TOF amp Q TOF Supplies ES TOF tuning mix 100 mL APCI L Low Concentration Tuning Mix 100 mL MMI L Low Concentration Tuning Mix 100 mL ES TOF reference mix 6 x 2 mL ampoules ES TOF Biopolymer reference standard kit LC MSD Common Chemical Supplies Flushing solvent High purity water 4 L Methyl alcohol 1 L Acetonitrile 1 L Formic acid 5 mL Ammonium formate ES APCI positive ion performance standard 5 x 1 mL ampoules These parts are common to all LC MSD systems 74 Order online at www agilent com chem store Part No G2421A G2432A G2424A 61946 85020 G2431A G2432A G1969 85000 G1969 85010 G1969 85020 G1969 85001 G1969 85003 G1969 85026 8500 2236 8500 1867 G2453 85050 G2453 85060 G1946 85021 G2423A LC MSD Common Supplies Description Part No Common Parts Filter element 5 jim box of 5 0100 2051 Rotor seal Tefzel pH 0 to 14 0100 1854 Rotor seal Vespel pH 0 to 10 0100 1855 Inlet filter assembly G1946 60180 SSV long drain tubing assembly G1969 60086 Cleaning Supplies Abrasive mesh 4000 grit 8660 0827 Capillary cleaning wire f
69. rmance and reliability 12 Order online at www agilent com chem store Maintaining the MSD Simple Repairs Procedure Flush the nebulizer Clean the electrospray spray chamber Replace the electrospray nebulizer needle Clean the APCI spray chamber Replace the APCI nebulizer needle Clean the Multimode source Check calibrant levels LC MSD Supplies Description ES nebulizer assembly Used for analytical sprayer API ES nebulizer needle APCI nebulizer assembly APCI nebulizer needle Needle assembly Corona Needle APCI When to Perform Daily or at the end of each shift to flush traces of samples and buffers out of the tubing valves and nebulizer Daily or anytime you suspect carryover contamination from one sample or analysis to another When the needle is plugged Common symptoms of a plugged needle are increased LC back pressure off axis spraying or dripping from the nebulizer Daily or anytime you suspect carryover contamination from one sample or analysis to another When the needle is plugged Common symptoms of a plugged needle are increased LC back pressure or off axis spray from the nebulizer Daily or anytime you suspect carryover contamination from one sample or analysis to another or when you must access the end cap and capillary cap for cleaning and inspection Monthly or weekly if you tune the LC MS frequently Part No LC MSD LC MSD LC MSD Quad Trap TOF 8 0 TOF G1946 60098 s
70. s G2427A s 4 s G1946 60037 4 G2428A G1947 60103 s 4 G1947 20029 s 6510 Quadrupole Time of Flight LC MS E 6410 Triple Quadrupole LC MS r Bia V 4 V i 6300 Series lon Trap LC MS 6210 Time of Flight LC MS C eo LC MSD Supplies Description Capillary cap G1946A B C Capillary cap G1946D and G1956A B Capillary G1946A B C and G1956A Capillary G1946D G1956B Spring canted coil 0 25 in ID 0 53 mm 1 6 in tee low dead volume Stainless steel Syringe adapter Syringe pump 1 16 in finger tight PEEK fittings beige Female luer to female 10 32 adapter PEEK tubing Gas tight syringe Teflon luer lock High throughput skimmer 2 mm Skimmer 1 G1946A B C Skimmer 1 G1946D Skimmer 1 G1956A B Skimmer 2 G1946A B C and G1956A Only HED Electron Multiplier G1946A B C D Detector assembly G1956A B Electron multiplier replacement horn Replacement horn and dynode Delay calibrant ESI Tuning Mix 100 mL Part No 61946 20056 61946 20301 G1946 80009 09987 20040 1460 2571 0100 0969 9301 1291 3162 0178 0100 1516 0100 2304 0890 1915 0182 9710 G1969 20302 G1946 20089 61946 20302 G1956 20302 G1946 20087 G1946 80019 G1956 80000 05971 80103 G2441 80010 61946 85020 G2421A LC MSD LC MSD LC MSD TOF amp 0 TOF Quad v i gt o i gt oeo Trap i gt oeeo oe gt gt gt 9 i gt gt 9 O
71. s precise injection volumes and high quality data And by following a regular schedule of preventive maintenance you can ensure a lifetime of defensible results This section will give you step by step instructions on how to perform crucial procedures such as e Replacing the rotor seal Checking the stator Replacing the needle and needle seat Checking the finger caps Replacing the metering unit seal Cleaning the piston Cleaning the solvent waste path Checking the leak sensor Performing a pressure test You will also find the top quality Agilent supplies you need to keep your autosampler running at top efficiency Order online at www agilent com chem store 13 14 Rotor seal replacement is the most common injection valve maintenance procedure Indications include rotor seal blockages and cross port leaks You should Suspect a cross port leak if you notice a drop of mobile phase coming out of the needle when it rises to draw sample from a vial Or if you experience poor injection volume precision Cross port leaks can also be found by performing a pressure test Tools 9 64 Allen wrench to remove the stator face and disassemble the valve Metric hexagonal keys for the rest of the Autosampler screws 1 4 Wrench to remove the capillaries Injection Valve Maintenance Replacing the Rotor Seal For Standard and Well Plate Autosamplers G1313A G132 A G136 A Order online
72. s steel Sample loop 100 uL Sample loop 900 uL Part No 0063 6586 0063 6589 0905 1294 0062 8587 G1313 87301 G1313 68 11 G1313 8 30 G1313 87308 0100 0900 01078 87302 61313 87303 Autosampler trays G1313 27 29A 1100 1200 Series Autosampler Supplies Description 100 position tray for 2 mL vials thermostattable 40 position tray for 2 mL vials 15 position tray for 6 mL vials External vial tray for 17 vials disposal position Disposal Tube for external vial tray Standard needle seat PEEK 0 17 mm ID capillary 2 3 uL Needle assembly for use with PEEK seat Needle seat PEEK without capillary G1313A PEEK seat tubing 0 17 mm ID 100 mm 2 3 uL PEEK capillary 0 25 mm ID 160 mm connecting valve with metering device of G1313 autosampler PEEK loop capillary for 100 pL sample Extended loop capillary 0 25 mm 180 cm Needle arm kit Finger Caps 15 pk Waste adapter 1100 Series autosamplers Waste adapter 1200 Series autosamplers gray Waste tube Waste tubing 5 m 6 5 mm ID corrugated polypropylene Post column reaction kit for 1100 LC Order online at www agilent com chem store Part No G1329 60011 G1313 44512 G1313 44513 G1313 60004 61313 27302 G1313 8 102 G1313 8 203 G1313 8 104 G1313 87302 G1313 87306 61313 87309 61329 87302 G1313 68 13 0063 6506 61313 43206 G1313 43216 61313 87300 0062 2463 G1313 68712 21 6 mL Vials Caps and Septa for 1100 1200 Series Autosam
73. seal 2 inlet filters 1 cartridge for active inlet valve PM Kit for isocratic quaternary pump includes piston seal PIFE frits 2 gold seals and 2 outlet caps PM Kit for binary pump includes 4 piston seals PIFE frits 3 gold seals 2 sieves and 3 outlet caps PM Kit for seal wash option includes 2 wash seals and a pack of 6 wash seal gaskets 1100 1200 Pump accessory kit Bottle head assembly for F29 32 tapered inlet bottle Solvent reservoirs 1L Bottle head assembly for F29 32 tapered solvent bottle Solvent reservoir 1 liter F29 32 Bottle head assembly for screw bottle Solvent reservoir 1L Solvent reservoir 1L with cap Solvent reservoir amber 1L For use with bottle head assembly Bottle cap with 3 hole insert Solvent mixer 1100 Series Capillary damper to purge valve Tubing kit degasser to pump 4 pk 30 cm pieces of tubing with screws and bushings Seal keeper ceramic hipped 46 Order online at www agilent com chem store Part No G1311 68710 G1310 68730 61312 68730 G1310 68731 G1311 68705 G1312 68706 G1312 68 16 9301 0656 G1311 60003 9301 1420 9301 1421 9301 1450 0063 6531 61312 87330 G1312 67301 61322 67300 0042 8952 G1312B 1200 Series Binary Pump SL Description PM Kit for binary pump includes 4 piston seals PIFE frits 3 gold seals 2 sieves and 3 outlet caps Active inlet valve without cartridge Cartridge for active inlet valve 600 bar Outlet valve for binary
74. seal stator face Column Item Needle and needle seat rotor seal Column inlet fittings Detectors Item DAD and MWD Detectors VWD Detectors Flow cell Typical Schedule Every month Every 6 months Every 12 months Every 24 months Typical Schedule Every 100 500 injections Every 24 months Typical Schedule Every 12 months Ever 5 10 column changes Typical Schedule Exchange standard lamps after 1000 hours Exchange longlife lamps after 2000 hours Exchange lamp after 1000 hours Check cleanliness every 6 months Actions Comments Before re installation of the purge valve always check the gold seal and replace if deformed Never use the system without a filter installed When piston seals are exchanged check the surface of the piston for scratches and deposits Never re install a used piston seal Pistons can be cleaned with alcohol or toothpaste After exchanging the active inlet valve cartridge pump several mL until the system is stable Actions Comments Change the guard after 25 50 injections for dirty samples 100 250 injections for most samples and 500 injections for very clean samples Actions Comments Make sure you use rotor seals of the correct material depending on the operating pH PEEK fittings make changing inlet fittings fast and easy Stainless steel fittings are reliable and can be used at higher pressure Actions Comments A noisy baseline may indicate it is
75. stalled Loops are also available in stainless steel or PEEK from 5 uL to 5 mL 10 mL for PEEK e Make Before Break MBB technology allows switching without flow interruption e Wide 30 port angles offer easier access to fittings e Built in position sensing switch provides the chromatograph with a reproducible Start signal 7725 manual injection valve 4 Order online at www agilent com chem store Series 37251 038 and 37251 Preparative Injection Valves The series 37251 038 stainless steel and 3 251 PEEK are the most suitable manual valves for large sample volumes high flow rates and preparative columns sized 1 0 10 cm in diameter Versatile ports accommodate 1 8 in 3 2 mm and 1 16 in 1 6 mm OD tubing Note 1 16 in OD tubing requires an adapter PN 5067 1503 1 0 mm diameter passages allow flow rates up to 800 mL min with virtually no pressure drop Make Before Break technology allows switching without flow interruption High reproducibility for both partial filling and complete filling methods Sample range is 100 uL to 20 mL 10 mL loop is installed Flow range is 10 to 800 mL min Built in position sensing switch gives the chromatograph a reproducible Start signal Description 77251 stainless steel 97251 PEEK 3 251 PEEK prep valve Manual prep injection valve kit stainless steel Manual Injection Valves with Position Sensing Switches Part No 0063 6502 0101 125
76. t Includes 2 windows 2 gaskets 1 2 gaskets 2 Cell screw Conical spring D version Ring stainless steel D version Gasket 1 PTFE D version Quartz windows D version Gasket 2 PIFE Replacement Parts Item Description zm gt J oj m Semi micro flow cell assembly 6 mm 5 uL 40 bar semi micro flow cell kit Includes 2 windows 4 gaskets 2 standard 1 1 semi micro 1 1 semi micro 2 Cell screw Conical spring D version Ring stainless steel D version Quartz windows D version Unit 10 pk 2 pk 10 pk 2 pk 10 pk Unit 10 pk 2 pk 2 pk Part No G1314 65050 79853 27200 79853 29100 79853 22500 79853 68743 79853 68742 G1314 65051 Part No G1314 60083 G1314 65056 79853 27200 79853 29100 79853 22500 79853 68742 G1314 60081 Micro Flow Cell Replacement Parts Item Description Unit Micro flow cell 3 mm 2 uL 120 bar for use with 1200 SL Micro flow cell 5 mm 1 uL 40 bar Micro flow cell kit Includes 2 windows 2 gaskets 1 2 gaskets 2 1 Cell screw 2 Conical spring D version 10 pk J Ring stainless steel D version 2 pk 4 Quartz windows D version 2 pk O Gasket 1 PTFE D version 10 pk 6 Gasket 2 PIFE 10 pk G1314 60082 High Pressure Flow Cell Replacement Parts Item Description High pressure flow cell 10 mm 14 uL 400 bar High pressure flow cell kit Includes 2 windows 2 Kapton gaskets 2 PEEK rings 1 Cell screw 2
77. teel union tubing and other parts Accessory kit for prep pump G1361A Includes stainless steel connecting capillaries wrenches 2 L solvent bottle bottle head assembly filter glass stop valve stainless steel union tubing and other parts PM kit for prep pump Includes 3150 0942 filter 5022 2192 filter assembly 5065 4445 peristaltic pump 0890 1 64 tubing and 4 prep pump seals Hi flow inline filter kit glass 10 um Contains glass filter caps and connecting tubing Hi flow replacement inline filter glass O ring Viton 30 mm Glass filter solvent inlet 40 um pore size Frit adapter PTFE for 4 7 mm OD tubing 9065 4500 Stainless steel filter assembly with PEEK ring 2 um pore size 48 Order online at www agilent com chem store Includes stainless steel connecting capillaries solvent mixer 2 L solvent bottle Part No G1361 68707 G1361 68708 G1361 68710 9065 4500 0065 9901 0905 1516 3150 0944 61361 23205 0022 2192 9065 9909 G1361A 1100 1200 Series Prep Pump Description Solvent botile clear 2 L 2 inlets Solvent bottle amber 2 L Solvent bottle clear 2 L Bottle head assembly for prep system Sapphire plunger Piston seal for G1361A Preparative Pump Frit adapter Peristaltic pump Valve assembly inlet outlet Stainless steel capillary outlet valve to 1 multi assembly 0 6 x 173 mm male male Stainless steel capillary outlet valve 1 to multi assembly 0 6 x 1 5 mm
78. time to change the lamp Low light intensity can be caused by a dirty flow cell Low light intensity can be caused by a dirty flow cell Low light intensity can be caused by a dirty flow cell How to contact Agilent For the latest information on the complete line of Agilent Technologies columns and supplies e Visit our Web site www agilent com chem e Contact your local Agilent sales office e Contact your local Agilent Authorized Distributor e In the U S and Canada call 1 800 227 9770 sales Option 1 then 1 Technical Support Option 4 then 2 Information descriptions and specifications in this publication are subject to change without notice Agilent Technologies Inc 2007 Printed in the USA October 15 2007 0989 7477EN
79. ts 4 piston seals 1 outlet gold seal 2 inlet filters 1 cartridge for active inlet valve PTFE frits 5 pk Gold seal outlet Outlet caps 4 pk Purge valve assembly Order online at www agilent com chem store Part No G1311 68710 01018 22707 0001 3707 0062 2485 G1311 60009 Cleaning or Exchanging the Outlet Ball Valve You should change the outlet ball valve as part of your regular maintenance routine or when the valve is leaking internally Typically if the pressure ripple is unstable you should run a leak test to determine if the outlet ball valve needs to be replaced 1 Remove the capillary trom the valve Outlet ball valve parts 2 Use a 14 mm wrench to remove the valve 3 Remove the plastic cap and gold seal 4 Clean the ball valve in an ultrasonic bath Or replace with a new valve 5 Replace the gold seal and cap 6 Use a 14 mm wrench to reinstall the valve Outlet Ball Valve Description Part No Outlet ball valve quaternary isocratic G1311 60012 Outlet ball valve for binary pump 400 bar G1312 60012 Gold seal outlet 5001 3707 Outlet caps 4 pk 0062 2485 Binary pump outlet valve SS sieve 10 pk 0063 6505 Order online at www agilent com chem store 39 You should service the active inlet valve when the pressure ripple is unstable and a leak test confirms a problem with the valve due to internal leaking The active inlet valve contains a cartridge that you can exchange as fo
80. with Needle Seat Standard needle seat 0 17 mm ID capillary 2 3 uL Standard needle seat 0 12 mm ID capillary 1 2 uL Standard needle seat PEEK 0 17 mm ID capillary 2 3 uL Standard needle seat 0 17 mm ID capillary 2 3 uL Micro LC Needle seat 100 um ID capillary 1 2 uL Micro LC Needle seat 90 um ID capillary 0 3 uL Needle seat well plate autosampler 0 17 mm ID capillary 2 3 uL Needle seat well plate autosampler 0 12 mm ID capillary 1 2 uL Needle seat micro well plate autosampler without seat capillary Seat Capillary 100 um Fused silica PEEK with fittings Seat Capillary 75 jim Fused silica PEEK with fittings Seat Capillary 50 jim Part No G1313 87101 G1313 8 103 G1313 8 102 G1313 87101 G1329 87101 61329 87103 G1367 87101 G1367 87102 G1377 87101 G13 5 8 31 G13 5 8 316 G13 5 87300 Twin needle seat dual loop autosampler G2258 87102 Needle seat prep autosampler 0 5 mm ID 20 uL G2260 87101 Autosampler Parts and Supplies all 3 Agilent offers a variety of autosampler supplies that increase the flexibility of your gt 1100 1200 System 1100 1200 Series Autosampler Kits Description Part No AEI PM Kit for 1200 Autosampler SL G1329 68719 Includes PEEK rotor seal needle seat needle 2 seals and 15 finger caps G1313 27A Autosampler maintenance kit G1313 68709 Includes 1 rotor seal Vespel 1 needle 1 needle seat 2 metering seals 15
81. with glass inserts 0 35 mL caps septa Glass inserts 350 uL Caps septa for glass inserts Vial plate for 54 x 2 mL vials Tray for 27 Eppendorf safe lock tubes 0 5 1 5 2 mL Vial plate for 15 x 6 mL vials Plate for 24 tubes with 18 mm diameter 1100 1200 Fraction Collector Capillary Kits and Needles 1100 1200 Module G1364B G1364C G1364D Max Flow Rate 100 mL min 1 mL min 10 mL min 10 mL min 100 mL min max 4 jiL min 4 30 jiL min 30 100 jiL min Tubing ID mm Tubing Kit Needle Length 0 8 mm ID G1364 68 11 0 15 mm ID G1364 68723 90 mm 0 25 mm ID G1364 68712 90 mm 0 25 mm ID G1364 68712 20 mm 0 8 mm ID G1364 68711 20 mm 29 ym ID G1364 87 304 90 ym ID G1364 87305 100 pm ID G1364 87306 Order online at www agilent com chem store Needle G1364 87201 G1367 87200 G1367 87200 61364 87202 61364 87202 Unit Part No 120 pk 5042 1385 10 pk 5042 1386 90 pk 5042 6454 90 pk 5042 1389 25 pk 5042 8502 30 pk 5042 1388 29 pk 5188 5375 0065 4402 1000 pk 5188 5321 1000 pk 5188 5322 6 pk G2255 68700 0022 6538 0022 6539 0042 8544 Typical Use Tubes max 100 mm Tubes max 48 mm well plates vials Funnel tray tubes max 75 mm MALDI targets well plates Micro Fraction Collector Spotter Maintenance Agilent offers three types of connecting capillaries each with different internal diameters We recommend replacing the capillaries and flap septum every six months o
82. ww agilent com chem store Part No G1315 60015 79883 68703 79883 27101 1000 0953 79883 28802 0062 8553 79883 22404 G1315 8 325 G1315 87306 G1315 84901 0515 1056 G1315 8 312 G1315 87311 79883 68700 69 DAD MWD Nano Flow Cells Nano Flow Cell Replacement Parts Item Description Unit Part No 900 nL Flow Cell G1315 68724 Contains quartz flow cell with 10 mm path length and 500 nL volume connecting capillaries max 50 bar pressure 80 nL Flow Cell G1315 68716 Contains quartz flow cell with 6 mm path length and 80 nL volume connecting capillaries max 50 bar pressure 40 cm long 50 jim ID 2 Fused silica PEEK capillary G1315 68708 70 cm long 75 um ID 3 Fitting screw 10 pk 5063 6593 4 Double winged nuts and 1 32 in 10 pk 5065 4422 ferrules O 1 32 in ferrule and SS lock ring 10 pk 5063 6592 lite touch 6 Union Adjustment Tool 2 pk 5022 2146 7 Universal ZDV union stainless steel 2 pk 5022 2184 no fittings 500 nL flow cell and replacement parts 8 Torque Wrench Adapter 61315 45003 9 Open end wrench 4 mm 8710 1534 70 Order online at www agilent com chem store Parts for the G1315 68724 500 nL Flow Cell Description Fused silica PEEK capillary 100 um ID 30 cm long Inlet Fused silica PEEK capillary 50 um ID 40 cm long Inlet Fused silica PEEK capillary 100 um ID 12 cm long Outlet Fused silica PEEK capillary 50 um ID 12 cm long Outlet Sealing kit Includes torque adapter
83. y or convenience Chances are you are under increased pressure to generate conclusive data under demanding deadlines That is why Agilent s ZORBAX RRHT line now includes columns that can be used at pressures of 600 bar for faster higher resolution separations Together with the Agilent 1200 Series Rapid Resolution System ZORBAX RRHT 1 8 jim HPLC columns can help you e Reduce analysis time by up to 95 e Process samples up to 20 times faster e Increase resolution by 30 40 over conventional HPLC e Develop HPLC methods more quickly e Analyze complex separations on shorter columns and with less solvent e Confidently move methods from your lab to any lab in the world e Perform conventional fast and ultra fast separations on the same unit e Securely transfer conventional methods and re evaluate current methods on fast LC without changing separation conditions To learn more about Agilent s 1200 Series LC system or our Rapid Resolution High Throughput columns consult your Agilent LC Column Selection Guide publication number 5989 5997 Or visit www agilent com chem 1200RR 2 Order online at www agilent com chem store Manual Injection Valve Maintenance Engineered for consistent results and optimal system performance Agilent s industry leading Manual Injection Valves are designed to ensure trouble free operation with your 1100 or 1200 Series HPLC System Our valves also feature patented Make Before Brea

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