- Advanced Cell Diagnostics
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1. 2 Open the oven door and use the provided offset screwdriver to unscrew the three screws shown in Figure 15 Figure 15 Remove the three screws shown with the screwdriver 3 Remove the latch and retain all three screws and associated washers In some ovens there may be one or more thin metal sheets under the latch These serve as spacers to adjust the torque needed to lock the latch Figure 16 Figure 16 Removed screws washers latches and offset screwdriver E Es Removed Latch Offset Screwdriver Clean the area around the three holes of the latch with 70 EtOH 5 Replace the plate and new latch Screw down with the washers and screws IMPORTANT Make sure screws are tight but do not overtighten or the washer will break 6 Replace the Frame Assembly Ensure that the back legs are in the holes in the back and the front legs are in the holes on either side ot the latch 20 ACD Hyb EZ Hybridization System User Manual PAD 7 To check that everything has been replaced properly place the HybEZ Humidity Control Tray in the Oven and close the latch 8 It the Frame Assembly does not latch remove the frame and reseat it making sure that the legs are in the holes 9 Repeat Step 7 Lubricate the Latch An eccentric wheel mechanism is used to lock down the clamp If not properly lubricated the wheel may wear out reducing the clamping force on the lid of the humidity control tray Loss of h2. hot during use Les surfaces peuvent devenir br lantes pendent l usage Caution Attention Risk of electric shock Before attempting any service to this unit remove power cord trom the rear of the unit Risque lectrique Debrancher la prise arri re de r parer l appareil Always observe the following safety precautions e Use only as specified by the operating instructions or the intrinsic protection may be impaired After transport or storage in humid conditions dry out the unit before connecting it to the supply voltage During drying out the intrinsic protection may be impaired e Connect only to a power supply with a voltage corresponding to that on the serial number label e Connect only to a power supply that provides a safety ground terminal e Before moving disconnect at the power supply socket Do not remove the plug e Do not check the temperature by touch but instead use the temperature display e lo reduce the risk of eye injury during high temperature operation use safety goggles or spectacles e Do not touch surfaces that become hot during high temperature operation e Ensure that the operating temperature is less than the maximum operating temperature of your sample material e Ensure that the power switch is easily accessible during use ACD Hvb EZ Hybridization System User Manual 23 24 It liquid is spilled inside the unit disconnect it from the power supply and hav
3. temperature of the HybEZ Oven is preset at 40 C when shipped which is the required temperature for RNAscope assays However the HybEZ Oven can be set at to different temperatures for other applications The Temperature Controller has three buttons that are used to control and display the temperature of the HybEZ Oven Figure 1 To display the set temperature depress the left button To lower the set temperature value depress the left button button VU simultaneously with the middle To raise the set temperature value depress he left button simultaneously with the right button A To display the current chamber temperature release all of the buttons Note In the event of power loss the Temperature Controller retains the last set temperature value ACD Hvb EZ Hybridization System User Manual AED Loading Tray with Slides To prepare the HybEZ Humidity Control Tray 1 Place HybEZ Humidifying Paper at center on the bottom of the tray and add approximately 50 ml of distilled water or current incubation solution on the paper 2 load HybEZ Slide Rack with up to 20 standard glass slides Figure 2 Figure 2 Slides loaded on Slide Rack 3 Use your standard protocol to prepare the slides with probe and buffer A Place the loaded Slide Rack into the Humidity Control Tray Loading Tray into Heating Chamber To insert the HybEZ Humidity Control Tray into HybEZ Oven 1 Open the main door of
4. the Oven lift the frame and pivot the latch forward Figure 3 Figure 3 Tray to be inserted _ l Heated LC AAA Chamber Frame lt Assembly gt lt D Latch Lae Tray Assembly 2 Slide the Tray into the Frame Assembly until it is tully seated against the rear of the Frame Assembly Figure 4 Figure 4 Tray inserted 10 ACD Hvb EZ Hybridization System User Manual PED 3 Rotate the latch into a vertical position and position the latch over the keeper on the frame Rotate the Latch Handle 180 clockwise Figure 5 Figure 5 Fully inserted and locked E E mi E E E SE Latch u Handig A 4 Rotate the Latch Handle down Figure 6 Close the main door Figure 6 Ready to close main door gt Latch N d l Handle Removing Trav from Heating Chamber IMPORTANTI Extreme care must be used when removing the tray due to potentially high temperatures The use of gloves may be necessary To remove HybEZ Humidity Control Tray from the HybEZ Oven 1 Open the main door of the Oven lift the Latch Handle up and rotate it 180 in a counter clockwise direction to unlock the latch 2 Hip the latch down lift the frame up and carefully slide the Tray Assembly out ACD Hvb EZ Hybridization System User Manual 11 12 Troubleshooting Issue Possible Cause Recommended Action Unit does not operate Unit not switched on Unit not plugged in
5. the power e For spills use the appropriate radiation chemical or biohazard clean up procedures e Clean the outer casing with water and a damp cloth e Do not submerge or immerse the HybEZ Oven in water e Before using any cleaning or decontamination method except those recommended by the manufacturer check that the proposed method will not damage equipment Removing the Frame Assembly If a spill occurs in the heating chamber it may be necessary to remove the Frame Assembly 1 Lift the front of the Frame Assembly so that the Frame Legs are out of the mating holes Figure 7 Figure 7 Lifting up the Frame Assembly Heating Chamber Assembly Frame Legs ACD Hvb EZ Hybridization System User Manual 13 AED 2 Pull the Frame Assembly forward Figure 8 Figure 8 Removing the Frame Assembly Alignment Pin Mating Hole pr s Heating Chamber Alignment gt T lt Pin Assembly Pull Frame Replacing the Frame Assembly To reinstall the Frame Assembly I Position the frame in the heating chamber so that the rear legs ot the Frame Assembly align with the holes in the block on the rear wall of the Oven Push the Frame Assembly toward the rear until it is fully seated Lower the Frame Assembly until the legs are seated in the mating holes in the block on the bottom surface of the Heating Chamber Replace the Latch To ensure that the latch properly functions we recommend replacing th
6. E E EE EE 6 Chapter 2 Assembly 7 SS 7 7 Chapter 3 Operation 9 Controls and Indicator Lamps Lee nee nn 9 Setting the Temperature ss sssesennsennznnnzznznnzznnznnzzznzznnzzznzzznzzznzzznzzzzzzzzzzanz 9 Loading Tray with Slide 10 Loading Tray into Heating Chamber 10 Removing Tray from Heating Chamber 11 WOES ET 12 Appendix A Maintenance for HybEZ Ovens Purchased Prior to DO A 13 Cleaning the HybEZ Owen 13 Removing the Frame Assembly eres 13 Replacing the Frame Aesembhy 14 Replace the Loch 14 PSI Vt ne o a 16 Replace nen ee 17 Appendix B Maintenance for HybEZ Ovens Purchased After LOK E 19 Cleaning the HybEZ Owen 19 Replace the ld 19 Lubricate lhe TTS 21 Replace Fuses 22 ACD HybEZ Hybridization System User Manual Appendix C Soe 23 EE 23 Documentation and support cccssssssceeeccceeeeessssseeeeeeeeeeeeens 25 Eed 25 KEEN 25 Warranty and Returns cccccccccccsscessccseesecesecssecssesseeeecasesseeseseseenseeesees 25 Limited product warranty uuuessnseneneennennnnnennnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nenn 25 EG 25 ACD Hvb EZ Hvbridization System User Manual Chapter 1 Product Information Before using this product read and understand the information in Appendix C Safety on page 23 in this do
7. USER MANUAL ACD HybEZ Hybridization System Document Number 310016 Rev E For Molecular Biology Applications MBA not intended diagnosis Refer to appropriate regulations Rev Date 20141205 For Molecular Biology Applications MBA not intended for diagnosis Refer to appropriate regulations Trademarks RNAscope and HybEZ are trademarks or registered trademarks of Advanced Cell Diagnostics Inc All other trademarks belong to their respective owners Citing HybEZ in Publications When describing a procedure for publication using this product please refer to it as the ACD HybEZ Hybridization System Cat No 310010 and Car Nox 310013 Disclaimers Advanced Cell Diagnostics Inc reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without notice Although this manual has been prepared with every precaution to ensure accuracy Advanced Cell Diagnostics Inc assumes no liability for any errors omissions or for any damages resulting from the use of this information Copyright 2014 Advanced Cell Diagnostics Inc All rights reserved Contents Chapter 1 Product Information rr 5 About this guide 5 Produc deso 5 Background RD DDR 5 Intended UE 5 HEH 6 Technical Specifications sss 6 CERA a E E E 6 PS TC ARONS a a E A E
8. Z Hybridization System User Manual 17 18 N D ACD Hvb EZ Hybridization System User Manual PED Appendix B Maintenance for HybEZ Ovens Purchased After 2013 The HybEZ Oven is designed to comply with IEC1010 1 The system is highly reliable and requires minimal maintenance Cleaning the HybEZ Oven If the HybEZ Oven requires cleaning first disengage the power cord e For spills use the appropriate radiation chemical or biohazard clean up procedures e Clean the outer casing with water and a damp cloth e Do not submerge or immerse the HybEZ Oven in water e Before using any cleaning or decontamination method except those recommended by the manufacturer check that the proposed method will not damage equipment Replace the Latch To ensure that the latch properly functions we recommend replacing the latch every two years for high frequency users who run the assay more than four times a week Order HybEZ Latch Replacement Kit Cat No 321400 directly trom ACD or through your distributor Figure 14 Locking latch that clamps down the Humidity Control Tray latch ra o Del ni 3 Fx Ni PU 3 i 1 E x 1 ud 4 gt gium 1 E m d e Wo be KR i ke WC i ei ACD Hvb EZ Hybridization System User Manual 19 PED To replace the latch on ovens manufactured ofter 2013 1 Turn off the HybEZ Oven and unplug from the outlet
9. cument IMPORTANT We recommend reading the entire user manual before beginning any protocols About this guide This user manual describes how to use the ACD HybEZ Hybridization System Product description Background Many staining assays that detect molecular markers in microscope mounted biological specimens involve repeated incubation steps at elevated temperatures where slides are held in a horizontal position that allow a small volume of reagents to incubate with the specimen During incubation special measures have to be taken to prevent loss of reagent due to evaporation Intended Use The ACD HybEZ Hybridization System is an evaporation controlled incubator designed to conduct the hybridization and incubation steps in Advanced Cell Diagnostic s proprietary RNAscope assays The HybEZ Humidity Control Tray and HybEZ Slide Rack included in the system allow batches of slides to be processed at the same time The ACD HybEZ Hybridization System is compatible with other hybridization based assays or any assay step where a condition of high humidity with an elevated temperature is required ACD Hvb EZ Hybridization System User Manual Components The ACD HybEZ Hybridization System contains the following components e HybEZ Oven 110 VAC Cat No 310010 220 VAC Cat No 310013 e HybEZ Humidity Control Tray with lid Cat No 310012 e HybEZ Slide Rack Cat No 310014 e HybEZ Humidifying Paper Pack with 15 sheets Ca
10. e it checked by a competent person It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilled on or inside the equipment The Power Cord supplied with the unit is the disconnect means ACD Hyb EZ Hybridization System User Manual Documentation and support Obtaining support For the latest services and support information go to www acdbio com product support html At the website you can e Access telephone and fax numbers to contact Technical Support and Sales facilities e Search through frequently asked questions FAQs e Submit a question directly to Technical Support e Search for user documents MSDSs application notes citations training videos and other product support documents e Find out information about customer training events Contact information Advanced Cell Diagnostics Inc 3960 Point Eden Way Hayward CA 94545 Toll Free 1 877 576 3636 Direct 1 510 576 8800 Fax 1 510 576 8801 Information info acdbio com Orders orders acdbio com Support Email support acdbio com Warranty and Returns Limited product warranty Advanced Cell Diagnostics Inc and or its attiliate s warrant their products as set forth in ADC General Terms and Conditions of Sale found on the ADC website at www acdbio com product support html When used in laboratory conditions and according to this User Manual Advanced Cell Diagnostics ACD warrants this product to be fre
11. e latch every two years for high frequency users who run the assay more than four times a week Order the HybEZ Latch Replacement Kit Cat No 321400 directly trom ACD or through your distributor Figure 9 Locking latch that clamps down the Humidity Control Tray latch en GA 3 Jr o kal l BZ WC We x 14 ACD Hvb EZ Hybridization System User Manual To replace the latch on ovens manufactured prior to 2013 1 Turn off the HybEZ Oven and unplug from the outlet 2 Open the oven door and use the provided Allen wrench to unscrew the three screws shown in Figure 10 Figure 10 Use Allen wrench to remove the three screws 3 Remove the latch and retain all three screws and associated washers In some ovens there may be one or more thin metal sheets under the latch These serve as spacers to adjust the torque needed to lock the latch Figure 1 1 Figure 11 Removed screws washers spacer latch and Allen wrench Removed Latch Screw Washer Spacer Clean the area around the three holes of the latch with 70 EtOH Replace the plate and new latch Screw down with the washers and screws IMPORTANT Make sure screws are tight but do not overtighten or the washer will break Replace the Frame Assembly Ensure that the back legs are in the holes in the back and the front legs are in the holes on either side of the latch ACD Hvb EZ Hybridization System User Manual 16 To chec
12. e of defective material and workmanship for a period of two years from the date of manufacture The liability of ACD for any defective equipment during the warranty period shall be limited to the repair of such equipment or replacement thereot without charge for parts or labor Returns A Returned Goods Authorization RGA number must be obtained before any ACD products are returned for any reason A Decontamination Notice must be completed signed by the user and returned to ACD prior to receiving the RGA number Please be sure to mark the outside of the return goods package with this RGA number to ensure prompt handling ACD Hvb EZ Hybridization System User Manual 25 Headquarters f e 3960 Point Eden Way Hayward CA 94545 Phone 1 510 576 8800 Toll Free 1 877 576 3636 A Nu For support email support acdbio com www acdbio com
13. k that everything has been replaced properly place the HybEZ Humidity Control Tray in the Oven and close the latch 8 It the Frame Assembly does not latch remove the frame and reseat it making sure that the legs are in the holes 9 Repeat Step 7 Lubricate the Latch An eccentric wheel mechanism is used to lock down the clamp If not properly lubricated the wheel may wear out reducing the clamping force on the lid of the humidity control tray Loss of humidity control in the tray leads to sample drying during hybridization steps and the generation of high staining background To maintain latch lubrication add one or two drops of general purpose lubricant such as WD 40 every three to six months to the back side of the latch Figure 1 2 Figure 12 Apply lubricant ACD Hyb EZ Hybridization System User Manual Replace Fuses Two supply fuses are located in the fuse drawer To replace the fuses l Disconnect the unit from the power supply 2 Remove the plug from the socket in the back of the unit 3 Pull back on the fuse drawer Figure 13 Figure 13 Fuse Drawer Fuse Drawer Pull out the fuse drawer 5 Check fuses and replace if necessary Replacement fuses must be 5 mm x 20 mm quick acting rated 250 V e 110V model 2 AF e 220 V model 1 AF Push the fuse drawer back in Reconnect unit to the power supply ACD Hvb E
14. t No 310015 The system can hold up to twenty standard microscope slides on the slide rack The unit has an advanced PID temperature controller that provides stable and accurate chamber temperatures trom ambient plus 10 C to 75 C The unit utilizes a sealed tray to create a lasting humid environment for overnight runs or weekend runs depending on the operating temperature Technical Specifications General This equipment is intended for indoor use and will meet performance specitications within the ambient temperature range of 10 C to 75 C with maximum relative humidity ot 80 non condensing Installation Category Il transient voltages Pollution Degree 2 in accordance with IEC 664 Suitable for operation at altitudes of up to 6500 feet Specifications Temperature Range Setting Range Stability Overall Accuracy Temperature Display Resolution Supply Voltage Range Power Rating Heating Rate Dimensions WxDxH Ambient 10 C to 75 C 0 10 E 0 5 C 0 5 C at 40 C DS 115V 10 50 60 Hz 230V 10 50 60 Hz 155W Ambient to 40 C within 20 minutes 17378 X 17 X ACD Hvb EZ Hybridization System User Manual 2 Unpacking Chapter 2 Assembly Remove packing materials carefully and retain for future shipment or storage ot the unit Inspect all materials for damage IMPORTANT Report all shipping damage to the carrier immediately Shipping damage is covered b
15. to power supply Fuses blown Power supply failure Switch on Plug in and switch on Replace fuses see Appendix A Maintenance Check that other electrical appliances on the same circuit are working Chamber temperature does not rise when expected Actual temperature is higher than set temperature Temperature control circuit fault Check set temperature Have unit checked by expert Temperature continues to rise when not expected Actual temperature is lower than set temperature Temperature control circuit fault Check set temperature Have unit checked by expert Slides dry out during heating cycle Gasket not sealing Lid bent Insufficient buffer placed in bottom of tray Latch not fully engaged Latch worn out see Appendix A Maintenance or Appendix B Maintenance for HybEZ Ovens Purchased After 2013 Replace Tray and Gasket Assembly Replace Lid Place more buffer in bottom of tray Reinstall Tray Assembly into chamber and retighten Latch Order and replace new latch Cat No 321400 ACD Hyb EZ Hybridization System User Manual Appendix A Maintenance for HybEZ Ovens Purchased Prior to 2013 The HybEZ Oven is designed to comply with IEC1010 1 The system is highly reliable and requires minimal maintenance Cleaning the HybEZ Oven IF the HybEZ Oven requires cleaning first disengage
16. umidity control in the tray leads to sample drying during hybridization steps and the generation of high staining background To maintain latch lubrication add one or two drops of general purpose lubricant such as WD 40 every three to six months to the back side of the latch Figure 17 Figure 17 Apply lubricant ACD Hvb EZ Hybridization System User Manual 21 22 Replace Fuses Two supply fuses are located in the fuse drawer To replace the fuses 1 Disconnect the unit from the power supply 2 Remove the plug trom the socket in the back of the unit 3 Pull back on the fuse drawer Figure 18 Figure 18 Fuse Drawer Fuse Drawer Pull out the fuse drawer 5 Check fuses and replace if necessary Replacement fuses must be 5 mm x 20 mm quick acting rated 250 V e 110V model 2 AF e 220 V model 1 AF Push the fuse drawer back in Reconnect unit to the power supply ACD Hvb EZ Hybridization System User Manual General safety Appendix C Safety The following symbols marked on the equipment mean Caution Attention Read these operating instructions fully before use and pay particular attention to sections containing this symbol Suivre attentivement les instructions avant l usage et pr tez une attention particuli re aux sections comportant ce symbole Caution Attention Surfaces can become
17. y the carrier and repair replacement for shipping damages must be coordinated through the carrier Packs should contain Installation HybEZ Oven Power Line Cord HybEZ Hybridization System User Manual HybEZ Humidity Control Tray includes a tray with gasket installed and lid HybEZ Slide Rack HybEZ Humidifying Paper To install the system 2 2 Place HybEZ Oven a flat and stable surface preferably away from drafts Fit the power line cord into the IEC power socket on the rear of the unit Plug power cord into a power supply that matches the voltage listed on the serial number label on the rear of the unit Prior to operation remove the tape inside the heating chamber that holds the frame assembly in place during shipping ACD Hvb EZ Hybridization System User Manual N D ACD Hvb EZ Hybridization System User Manual 3 Chapter 3 Operation Controls and Indicator Lamps The Power Switch controls power to the unit The temperature display shows the chamber temperature in degrees Celsius Temperature is preset at 40 C for the RNAscope Assay While the HybEZ Oven is heating up the heater lamp will be on The lamp will flash intermittently once the target temperature has been reached and is being maintained Figure 1 Controls and indicators Heater l La Temperature Display OOY COA Setting the Temperature The
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