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Microelectrode Array (MEA) Manual

1. MEA Manual MEA Types and Layouts Various types of MEA biosensors are available for all kind of extracellular multi channel recordings Typical MEAs for in vitro applications have 60 microelectrodes arranged in an 8 x 8 layout grid embedded in a transparent glass substrate You can cultivate the tissue or cell culture directly on the MEA EcoFlex and FlexMEAs are made for in vivo and in vitro applications MEA types differ in the materials used for the carrier and the recording area and in the geometry that is electrode size and interelectrode distances The electrode size and interelectrode distances are used for categorizing MEAs The first number refers to the interelectrode distance for example 200 um and the second number refers to the electrode size for example 10 um which results in the standard MEA type 200 10 for example Standard versions are available with an internal reference electrode abbreviated iR and with various culture chamber interface options Culture chambers are available with and without lid Please ask for custom layouts that is MEA layouts according to your specifications In this chapter each MEA type is briefly described and noted Standard MEAs with flat round TiN electrodes in an 8 x 8 layout grid for all applications MEAs with 6 x 10 layout grid and 500 um inter electrode distances HighDenseMEAs with the highest spatial resolution and a double recording field of 5 x 6 electrodes each He
2. 2 ground electrodes Upside Electrode ot MERE A field 00000 VANTIN Connect the EcoFlexMEA36 directly to a 32 Channel Miniature Preamplifier Insert the EcoFlexMEA36 into the MPA32I with the electrode field up when the backside of the MPA32I with I the screws is upside down The additional connector can be used 23 mm for connecting a silver pellet or a silver wire for grounding the bath 30 mm Technical Specifications EcoFlexMEA36 Temperature compatibility 10 125 C Dimension W x D 37 mm x 30 mm Thickness of the electrode field 50 um Weight 10g Base material Polyimide Kapton Contact pads and track material Gold Electrode diameter 50 um Interelectrode distance centre to centre 300 um Electrode height Planar Electrode type Gold electrodes Isolation type Polyimide Kapton Electrode impedance Approximately 50 kQ Electrode layout grid 6x6 Number of electrodes 36 Reference electrodes 2 internal reference electrodes Ground electrodes 2 ground electrodes MC Rack Source layout in Data Source Setup 1 dimensional 32 channels no digital channel Channel map EcoFlexMEA36 cmp Cleaning Rinse with distilled water EcoFlexMEAs made from Polyimide Kapton are heat stable and autoclavable Multi Channel Systems Fon 49 7121 9 09 25 O O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to ch
3. 1000 0 1928th sweep Ys 50 eV soo soo ny D uoo yn TUN PITT INTE aalis Temm lei a This picture shows the typical noise level of a standard 60MEA200 30iR Ti on most electrodes recorded with a MEA1060 BC amplifier 51 MEA Manual Electrodes 43 52 53 and 84 show an increased noise level after a longer cycle of use The bath was grounded with the internal reference electrode 15 Time axis 1000 ms voltage axis 50 uV You should ground some of the electrodes if you want to use this MEA for recording E Standard Noise with Shielding BEB 514 000sec XAxs 1000 ms gt oo J soo 5l4hsweep YAxis 20 gt w gt 200 J 100 150 200 250 300 350 stevia i tira Pe Pe Pe Pa r E continuous Oat E 1890 000sec xs 1000 p ms j 09 10000 1890th sweep Ys 100 pfu 1000 1000 500 0 500 0 500 0 poo 0 500 0 poo 0 500 0 500 srili ETE WYNER Crees Yee OI Pf Poco eaa Dos sliri T Same MEA after grounding defective electrodes Time axis 1000 ms voltage axis 100 uV Typical noise level of a standard 60MEA200 1 OiR Ti round 10 um Tin electrodes 3563000sec XAxis 1000 ms gt 00 3563rd sweep YAxis 100 uV gt 1000 Noise level of a new standard 60MEA200 10iR Ti Bath grounded with the internal reference electrode 15 Time axis 1000 ms voltage axis 100 pV 52 Stimulation 8 Stimulation
4. G9 9 G9 69 9 The numbering of MEA electrodes in the 8 x 8 grid standard MEAs ThinMEAs EcoMEAs StimMEAs pMEAs follows the standard numbering scheme for square grids The first digit is the column number and the second digit is the row number For example electrode 23 is positioned in the third row of the second column These numbers are the same numbers that are used as channel numbers in the MCS data acquisition software for example the MC Rack program Using MC Rack please make sure that you have selected the two dimensional MEA layout as the Channel Layout in Data Source Setup For more details please refer to the MC Rack manual or help Important MEAs are not symmetrical That is why the writing for example NMI LEITER MEA type on the MEA chip should be on the right side viewed from the front with the sockets of the amplifier in the back MEAs with one big internal reference electrode should be placed with reference electrode to the left side in the amplifier Otherwise the MEA layout will not match with the pin layout of the channel map in the data acquisition program Other electrode grids are described in the next chapter and in the Appendix 13 MEA Manual 4 2 Standard MEA 60MEA100 10 ITO 60MEA200 10iR ITO 60MEA200 30IR ITO 60MEA100 10iR TI GOMEA200 10 Ti 60MEA200 30 Ti 60MEA200 10iR TI GOMEA200 30IR Ti GOMEA500 10IR Ti G OMEA500 30iR Ti Standard MEAs have 60 electrodes in an 8 x 8 layout
5. MEA Manual Procedure Incubate the MEA with polyornithine solution at RT for 2 3 hours or overnight at 4 C Aspirate the polyornithine solution and rinse the MEA 3 x with distilled water before direct use or before the following coating with laminin MEAs coated with polyornithine can be stored at 4 C for several weeks Incubate pre coated MEA with laminin solution for at least 1 h Aspirate the laminin solution and directly plate cells Literature Cellular Neurobiology A practical approach ed By Chad and Wheal IRL Press Oxford Coating with Poly D Lysine plus Laminin Poly D lysine has been used by several groups Results seem to be equivalent to a coating with polyornithine Some users complained about cell clumping and resulting cell death when using poly D lysine and had better results when using polyethylenimine PEI Materials Poly D lysine 5 mg 10 mL 0 05 w v stock solution Sigma Aldrich Inc P7280 Laminin solution 1 mg ml Sigma Aldrich Inc L2020 Laminin solution 20 ug ml laminin in plating medium or PBS phosphate buffered saline Procedure Incubate the MEA with poly D lysine solution and incubate at 4 C over night Rinse MEA with sterile distilled water 3x to remove toxic unbound lysine and let the MEAs air dry under sterile conditions laminar flow before plating the cells or before the following coating with laminin MEAs can be stored at 4 C for up to two weeks Incubate
6. The same material is used for standard MEAs and 256 9wellMEAs Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures 25 MEA Manual 4 14120MEA with for Use with MEA2100 120 System 120MEA200 30iR Ti 120MEA100 30iR Ti 1209 MEA200 30iR Ti A 120MEA200 30iR Ti 120pMEA200 30iR Ti electrode field with perforation The 120MEA200 30iR Ti has 120 electrodes and can only be used with the MEA2100 System connected to a headstage HS120 with 120 electrodes Please refer to the MEA2100 System manual for detailed information The dimension of the glass carrier is 49 x 49 x 1 mm as usual The MEAs with 120 electrodes are stable in a temperature range from 0 125 C The 120MEA200 30iR Ti contains 120 recording four reference and four ground electrodes arranged in a 12 x 12 layout grid They are embedded in a transparent glass substrate The contact to the amplifier is provided by a double ring of contact pads around the rim of the MEA The standard material for MEAs is also used for 120MEA200 30iR Ti The electrodes are from titanium nitride TIN with a silicon nitride SiN isolator and contact pads and tracks are made of titanium nitride TiN The spacing between the recording electrodes in the 12 x 12 grid averages 100 or 200 um between the electrodes The electrode diameter of 30 um results in an impedance of approximately 30 50 kQ
7. 2 606600600068 00 06069660069 9e G3 G9 9 G9 6 9 v 28 37 38 145 46 48 47 57 58 56 55 68 67 78 Electrode s meapins 2 aim 6 6 6 6 6 G meapins sam The numbering of MEA electrodes in the 8 x 8 grid follows the standard numbering scheme for square grids The first digit is the column number and the second digit is the row number For example electrode 23 is positioned in the third row of the second column The specified MEA pin numbers 1 dim or 2 dim are the channel numbers that are used in the data acquisition program when using the 1 dimensional layout or the 2 dimensional layout or Configuration in the Data Source Setup The electrode 15 is missing in MEAs with internal reference electrode It is replaced by a big internal reference electrode connected to pin 15 of the amplifier Multi Channel Systems Fon 49 7121 9 09 25 O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 60MEA500 10iR Ti systems 60MEA500 10iR Ti 60MEA500 30iR Ti O O gt 000000 O00000 00000 0000 000000 0000 000000 MEA with electrode layout grid 6 x 10 Technical Specifications Micro Electrode Array with 6 x 10 Layout Temperature compatibilit
8. 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice PMEA32S12 Layout 2 multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System Technical Specifications pMEA32S12 Layout 2 Temperature compatibility Dimension W x D x H Base material Perforation Total area of holes Diameter of the holes Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of stimulation electrodes Number of reference electrodes MC_Rack Source layout in Data Source Setup Channel map 10 50 C 49 mm x 25 mm x 1 8mm Polyimide foil on ceramic carrier with perforation 0 8 mm 90 75 50 30 20 um Ti Titanium TiN Titanium nitride 30 um recording electrodes 50 um stimulation electrodes 90 um and 150 um recording electrodes 90 um and 150 um stimulation electrodes Planar TiN Titanium nitride electrodes Polyimide foil Approximately 30 50 kQ 1x10 1x12 1x 10 recording electrodes 6 x 2 stimulation electrodes 32 12 1 internal reference electrode Configuration MEA2100 32 1 dimensional no digital channel USB MEA32 S
9. 8 1 Using MEA Electrodes for Stimulation You can use any MEA electrode s for stimulation Simply connect the stimulus generator outputs to the MEA amplifier Please see the manual for the respective MEA amplifier and stimulus generator for more details As an alternative you can also use special 60StimMEA with four pairs of large 250 x 50 um stimulating electrodes and a special stimulation adapter or target cells with an external electrode for stimulation This and the following chapters are intended for helping you to optimize the stimulation with MEA electrodes All electrodes suffer under electrical stimulation especially under long term stimulation The wear depends on the stimulus and on the electrode type When stimulating via MEA electrodes and with standard MEA amplifiers you will see a stimulus artifact on all amplifier channels during stimulation due to the high charge that is injected into the circuit and the following saturation of the filter amplifiers The time constant of the stimulus artifact depends on the amplifier bandwidth if the lower cutoff frequency is quite low for example 1 Hz the stimulus artifact will be longer than with 10 Hz for example In most cases it will not be possible to record true signals that are close to the stimulus pulse This can be avoided by using a MEA amplifier with blanking circuit The stimulating electrode can generally not be used for recording in parallel to stimulation because the inject
10. Contact pads Indium tin oxide ITO or Titanium nitride TiN Electrode diameter 30 um Interelectrode distance centre to centre 200 um Electrode height Planar Electrode type Titanium nitride TiN electrodes Isolation type Silicon nitride 500 nm PEVCD Electrode impedance 30 50 kQ Electrode layout grid 8x8 and additional 4 pairs of large stimulation electrodes STIM 70 x 250 um 4 pairs of small stimulation electrodes S 30 um Number of recording electrodes 60 Number of reference electrodes Without internal reference electrode MC Rack Source layout in Data Source Setup 2 dim MEA Channel map Default MEA perfusion chamber w o without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60pMEA100 30iR Ti 60pMEA100 30iR Ti multichannel systems OO OOO 000000 000000 eeecco 000000 000000 000000 000000 000000 eeecco Perforated Microelectrode Array with 6 x 10 electrode layout grid Perforated MEAs for use with MEA1060 amplifiers equipped with a perfusion ground plate PGP or a MEA2100 headstage with a perfusion element PE The perforation allows a perfusion of the tissue from both sides of the pMEA Te
11. Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany gr Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com Carbon nanotube stucture of PEDOT electrodes 3 um 0 125 C 49 mm x 49 mm x 1 mm Glass Ti Au Titanium Gold 30 um 200 um Planar PEDOT CNT carbon nanotube poly 3 4 ethylene dioxythiophene Silicon nitride 500 nm PEVCD Approximately 20 kQ 8x8 59 1 internal reference electrode 2 dim MEA or Configuration Default Glass ring ID 19 mm OD 24 mm height 6 12 mm 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice FlexMEA36 multichannel systems Flexible Microelectrode Array with 36 electrodes for use with 32 Channel Miniature Preamplifier MPA32I Flex or with the ADPT FM 32 adapter and the standard MPA32lI Technical Specifications FlexMEA36 Temperature compatibility Dimension W x D Thickness of the electrode field Weight Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Perforation Electrode height Electrode type Isolation t
12. This MEA is also available as perforated 120pMEA200 30iR Ti The electrodes are from Titan Gold Titan all other MEA parameters are the same The inner diameter around the electrodes of 3 to 4 mm is perforated and the total area of holes is 12 96 of these area Using 120MEAs The same material is used for standard MEAs and 120MEA200 30iR Ti and 120MEA100 30iR Ti Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Using 120pMEA200 30iR Ti Perforated MEAs have a glass carrier but the electrodes are embedded in polyimide foil Therefore they are heat stable to 50 C only and cannot be autoclaved Please do not use an ultrasonic bath for cleaning 26 MEA Manual 4 15 Quadrant MEA 60 4QMEA1000 60 4QMEA1000iR Ti 0000 ooo wow url oor Oo O 9 i O O O OOO 2m oo 00000 The 60 4QMEA1000 has 60 electrodes organized in four quadrants 13 electrodes each with a center line 7 electrodes The electrode diameter is 30 um and the interelectrode distance varies Inside the quadrants the distance is 200 um from quadrant to quadrant the distance is 1000 um and to the center line it is 500 um The 60 4QMEA1000 is available with an internal reference electrode The flat round electrodes are made of titanium nitride TIN MEAs with TiN electrodes are very stable Therefore the MEAs can be reused several times an
13. 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm Glass ITO Indium tin oxide 10 um 40 um Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD Approximately 250 400 kQ Hexagonal 59 1 internal reference electrode iR 2 dim MEA HexaMEA40 10 cmp Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice multichannel 60HexaMEA40 10 systems 60HexaMEA40 10iR ITO Electrode Layout mars 0000000000000000 A2 C4 A4 Electrode C3 B2 Al B5 A5 E4 B6 B1 MEA pins 32 44 42 51 61 D6 e e e 10 um 2 3 Electrode A1 A2 A3 A4 A5 a C7 82 3 c B1 B2 B3 B4 B5 D 3 40 um 3 D3 23 12 33 41 53 63 82 E5 83 O O O e ai 93 T C1 C2 C3 C4 C5 c6 40 Hm C7 Bs 8 24 34 13 14 31 73 73 64 OQ e 9 GA Di D1 D2 D3 D4 D5 D6 D7 D8 EG 74 14 D3 25 35 37 62 83 74 84 E 84 e e e e amp is REF E1 E2 E3 E4 E5 E6 E7 F5 85 16 26 27 68 85 86 65 75 25 e1 0 9 eee 68 6 6 rs 75 F1 F2 F3 FA F5 F6 F7 F8 5 E2 17 36 46 58 66 87 76 E 65 e O O e 16 F1 G1 G2 G3 G4 G5 G6 G7 F6 8e T 45 47 56 78 4 D H1 2 3 e 5 H6 Os
14. C 49 mm x 49 mm x 1 mm 180 um Glass part Glass on ceramic carrier ITO Indium tin oxide 10 or 30 um 100 or 200 um Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD 30 50 kO for 30 um 250 400 kO for 10 um electrodes 8x8 59 with iR or 60 without iR 1 internal reference electrode iR 60ThinMEA200 30iR ITO or without internal reference electrode 60ThinMEA100 10 ITO 60ThinMEA30 10 ITO 2 dim MEA Default 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60EcoMEA multichannel systems Low priced Microelectrode Array on PCB Base 60EcoMEA on PCB base with 8 x 8 standard electrode layout amp OQ 6 O6 90 6600 KEKE 66600 900060 er 90660606 00000 CIT amp 6 6 60000 o66000000 Technical Specifications GOEcoMEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspen
15. Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Contact pads for reference electrodes connected to ground MC Rack Source layout in Data Source Setup MEA MEA perfusion chamber w o Without ring C2 D3 Di ES Ei F3 Fi G5 G2 G4 H2 H J2 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 7172 73 74 75 EE ue PIE S88298582992825 amp E C3 ES D2 E4 E2 F4 F2 F5G6 Gi G3 H Jt J3 K2 J4 ga L8 Z M AM a7 RN 97 ws ante 98 Le NN 99 ke 100 us 101 m9 102 1e a 103 ke 104 uo 105 110 G12 G10 H12 H10 G8 cen G9 H11 Hi 1514131211109 8 7 6 5 43 2 1 120119 118 117 116 115 114 113 112 111 110 109 108 107 106 D9 Dii E9 Ei F9 Ft F8 Ji2 J10 Kt Ci D10 D12 E10 12 F10 F12 F7 9 Ji H8 K10 is 0 125 C 49 x 49 x 1 mm Glass Ti Titanium or ITO Indium tin oxide 30 um 100 or 200 um Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD 30 50 kQ electrodes 12x12 120 4 internal reference electrodes iR 4 Configuration MEA120 200 30 gr Glass ring ID 19 mm OD 24 mm pr Plastic ring without thread ID 26 5 mm OD 30 mm height 3 6 mm pr T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Multi Channel Systems Fon 49 7121 9 09 25 0 MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems
16. FAL 30 um FSR 76 17 F2L ry FAR 87 F3L D3L F4L F5L E4L E5L D4L D5L DIR D2R E1R E2R FIR F2R D3R F3R The first letter of the electrode number code refers to the row number the digit is the column number and the second letter refers to the electrode field left or right of the 60HighDenseMEA The specified MEA pin numbers are the channel numbers that are used in the MC Rack program The electrode D1 of the left electrode field connected to channel 15 in MC Rack is missing It is replaced by a big internal reference electrode Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60HexaMEA40 10 60HexaMEA40 10iR ITO 60 Hexa Microelectrode Array multichannel systems ZS a Technical Specifications 60HexaMEA40 10 Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21
17. G13 Conn 4 No 21 H13 Conn 3 No 49 J13 Conn 3 No 21 connector socket G14 Conn 4 No 19 H14 Conn 3 No 47 J14 Conn 3 No 19 in well G H and J G21 Conn 4 No 28 H21 Conn 3 No 56 mi Conn 3 No 28 G22 Conn 4 No 25 H22 Conn 3 No 53 J22 Conn 3 No 25 G23 Conn 4 No 22 H23 Conn 3 No 51 J23 Conn 3 No 23 G24 Conn 4 No 20 H24 Conn 3 No 48 J24 Conn 3 No 20 G25 Conn 4 No 17 H25 Conn 3 No 45 J25 Conn 3 No 17 G31 Conn 4 No 30 H31 Conn 3 No 58 431 Conn 3 No 30 G32 Conn 4 No 27 H32 Conn 3 No 55 432 Conn 3 No 27 G33 Conn 4 No 23 H33 Conn 3 No 50 433 Conn 3 No 22 G34 Conn 4 No 18 H34 Conn 3 No 46 434 Conn 3 No 18 G35 Conn 4 No 15 H35 Conn 3 No 43 435 Conn 3 No 15 G41 Conn 4 No 1 H41 Conn 3 No 29 J41 Conn 3 No 1 G42 Conn 4 No 4 H42 Conn 3 No 32 J42 Conn 3 No 4 G43 Conn 4 No 9 H43 Conn 3 No 35 J43 Conn 3 No 8 G44 Conn 4 No 13 H44 Conn 3 No 41 J44 Conn 3 No 13 G45 Conn 4 No 16 H45 Conn 3 No 44 J45 Conn 3 No 16 G51 Conn 4 No 3 H51 Conn 3 No 31 451 Conn 3 No 3 G52 Conn 4 No 6 H52 Conn 3 No 34 452 Conn 3 No 6 G53 Conn 4 No 7 H53 Conn 3 No 37 453 Conn 3 No 9 G54 Conn 4 No 11 H54 Conn 3 No 39 454 Conn 3 No 11 G55 Conn 4 No 14 H55 Conn 3 No 42 455 Conn 3 No 14 G62 Conn 4 No 5 H62 Conn 3 No 33 J62 Conn 3 No 5 G63 Conn 4 No 10 H63 Conn 3 No 38 J63 Conn 3 No 10 G64 Conn 4 No 12 H64 Conn 3 No 40 J64 Conn 3 No 12 Gs2 Conn 4 No 8 Hs2 Con
18. Indium tin oxide ITO or Titanium Ti 10 20 30 um 30 60 90 um Planar Titanium nitride TiN Silicon nitride 500 nm PEVCD 30 kQ 50 kQ for 30 um electrodes 250 400 kQ for 10 and 20 um electrodes hexagonal 60 without internal reference electrode 2 dim MEA HexaMEA cmp Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice multichannel 60HexaMEA ITO 60HexaME A Ti Electrode Layout 550009000000000000 Electrode B7 B10 C5 C4 C2 C6 C8 Cl C7 C9 C10 D5 rie fede C5 C8 D5 Q2 B1 MEA1060 pins 31 52 62 D2 72 30 um D 12 B8 D6 C6 C9 D4 3 m B8 44 42 54 71 D8 D3 20 um B9 D6 T OY og e 9 e 3 12 43 53 63 73 21 82 B3 10 um D7 B6 B7 C2 c0 D2 D7 D9 24 B4 e e 41 61 72 O D9 74 23 33 8107 c1 9 p1 pio 64 74 B5 B4 T E4 E5 14 B5 B3 82 32 gi 51 pq 83 pi 94 p2 E3 D10 84 B oe 9 e e o e e e e 24 34 1341022 a138 f1 77 g19 86 65 75 S A10 14 i 85 E5 85 Wd A A7 15 az 16 1047 pa 67 E7 E6 Q Om ra 5 25 35 T 1 66 76 30 um S a7 A8 AG A3 F7 F3 ES E8 E3 65 2 OR i 2 26
19. L2 48 155 17 P8 29 49 103 L16 22 202 58 E11 18 230 208 L3 47 25 47 P9 38 180 71 M12 8 195 217 E12 8 225 142 L4 50 156 86 R10 42 182 72 M13 14 198 218 E6 49 125 230 L5 49 26 116 R11 45 57 99 M14 13 74 246 E7 46 244 199 L6 59 31 46 R12 52 187 1 M15 16 199 222 E8 39 120 163 M1 52 157 18 R13 55 62 29 M16 15 75 249 E9 28 235 137 M2 51 27 48 R14 62 192 65 N14 10 196 216 F10 11 106 174 M3 54 158 83 R2 4 163 13 N15 9 72 244 F6 59 130 160 M4 53 28 113 R3 5 37 109 N16 12 197 219 F7 56 249 132 N1 55 29 45 R4 12 167 11 014 3 69 28 F8 36 239 135 N2 58 160 84 R5 15 42 39 015 6 194 214 F9 31 116 165 N3 57 30 114 R6 22 172 75 016 5 70 64 G10 7 104 173 N4 63 33 43 R7 25 47 106 P15 1 68 96 G8 33 117 234 01 62 162 81 R8 32 177 8 P16 4 193 126 G9 34 238 204 02 61 32 111 R9 35 52 36 R15 2 67 66 H8 60 251 129 GND 64 253 GND 64 254 GND 64 255 GND 64 256 Stim Socket Stimulation socket number in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier Hardware ID Hardware channel ID of MC Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 16 x 16 layout grid Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without n
20. Optically control the MEA with a microscope If concentric colored rings Newton rings are visible due to light interference the insulation layer is too thin and you should use a fresh MEA The insulation layer has been abraded and is missing in parts This will result in a short circuit between the electrodes or tracks and the bath You will still see signals but as an unspecific smear over the complete array Usea fresh MEA 61 Appendix 10 Appendix 10 1 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site User forum The Multi Channel Systems User Forum provides an excellent opportunity for you to exchange your experience or thoughts with other users worldwide Mailing List If you have subscribed to the mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the mailing list on the contact form of the MCS web site www multichannelsystems com 63 MEA Manual 10 2 Safe Charge Injection Limits 64 Safe Charge Injection Limits of Micro Electrode Arrays with TiN Electrodes diameter 30 um max pulse amplitude UA max pulse amplitude n A max pulse amplitude LA safe charge injection limits 2000 1500 1000 500 0 50 100 150 200 250 300 350 400 450 500 time us safe charge injection limits 400 r r r T 350 300 250
21. connected to ground MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Without ring 0 125 C 49 mm x 49 mm x 1 mm 180 um Glass on ceramic carrier Indium tin oxide ITO 30 um 200 um Planar Titanium nitride TiN Silicon nitride SiN 30 50 kQ 16 x 16 252 4 internal reference electrodes iR 4 Configuration 16 x 16 cmp Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice multichannel 256ThinMEA systems A281 C2E50301E4E2 F5 FS F1G4G2 H5 H3 H1H7 16 12 4 KY K8 KS L2 L4 M1 M8 KG N2 18 O1GND B208 01G7D2G6 E83 E1 F4F2G5 G3 G1 H4 H2 H6 17 11 18 15 K2 K amp L1 L8 L5 M2 MANT N L6 O2 N4 Aid amp eo A3 B3 os Re Here Ra P3 B4c4 K7 M5 H 6666060606000060606 ee 2 amp amp 6 6606 60062B3t it3oosPs os ns so 906060606060606006606000060 Z7 6 amp amp amp on amp oeo000o909n Pece 2 amp 6060606660606o006260689 2 os Ee amp amp Gb 5 Eo o amp amp do 9 EHH amp v s ave a 000000000000000 de Go amp am
22. 38 48 57 55 67 ce 87 h 2 3 amp 2 F3 H6 H2 I2 13 H5 The letter digit code is the electrode identifier and refers to the position of the electrode in the hexa grid The specified MEA amplifier pin numbers are the channel numbers that are used in MC_Rack Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60HexaMEA 60HexaMEA ITO 60HexaMEA Ti 60 Hexa Microelectrode Array multichannel systems e o o e o e e e Qo e e eee o o 210 220 230 um e e eeeee o Q Q o e eee e Q e e 30 60 90 90 um e e e o e Technical Specifications 6G0HexaMEA Temperature compatibility 0 125 C Dimension W x D x H Thickness region of electrodes Base material Contact pads Track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC_Rack Source Layout in Data Source Setup MCS Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 49 mm x 49 mm x 1 mm Glass Indium tin oxide ITO or Titanium nitride TiN
23. 43 B24 23 50 43 C75 56 34 43 E63 89 175 43 F12 122 164 44 A45 153 20 44 c64 183 3 44 D42 213 189 44 F23 243 133 45 B34 24 115 45 C76 57 99 45 E53 90 178 45 F11 123 229 46 B14 154 85 46 C65 184 69 46 E73 214 192 46 F22 244 199 47 B23 25 47 47 c66 58 31 47 E64 91 177 47 F21 124 161 48 B13 155 17 48 c54 185 4 48 E74 215 191 48 F33 245 134 49 B22 26 116 49 C56 59 100 49 E65 92 180 49 F31 125 230 50 Bi2 156 86 50 C55 186 70 50 E75 216 145 50 F32 246 200 51 B33 27 48 51 C46 eo 32 51 E54 93 179 51 F41 126 162 52 B11 157 18 52 c44 187 1 52 E76 217 147 52 F43 247 131 53 B32 28 113 53 c36 61 97 53 E55 94 182 53 F51 127 227 54 B21 158 83 54 C45 188 67 54 E66 218 243 54 F42 248 197 55 B42 29 45 55 C26 62 29 55 E45 95 181 55 F61 128 159 56 B31 159 15 56 C35 189 2 56 E56 219 245 56 F52 249 132 57 B43 30 114 57 C16 63 98 57 E44 96 184 57 F71 129 228 58 B41 160 84 58 C34 190 68 58 E46 220 213 58 F53 250 198 59 B52 31 46 59 C15 64 30 59 E35 97 183 59 F72 130 160 60 B51 161 16 60 C25 191 125 60 E36 221 215 60 F62 251 129 61 B53 32 111 61 c14 65 95 61 E34 98 239 61 F73 131 225 62 B61 162 8
24. 48 6 6 Ring Options Recording with MEAs The following table shows all available ring options Glass rings gr are available in two heights of 6 or 12 mm Plastic rings pr are available in four heights and without or with thread pr T The triangle tcr and round rcr chamber rings are suitable for the 6 well MEAs the macrolon quadrates mq for the 252 9wellMEAs MEA Ring Options ALA MEA MEM ID 24 5 mm OD 31 mm ALA MEA INSERT m Rings standard Glass ring 12 mm high Plastic ring 3 mm high ID 19 mm OD 24 mm ID 26 5 mm OD 30 mm Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon 49 7121 9 09 25 0 Fax 449 7121 9 09 2511 infoQmultichannelsystems com www multichannelsystems com Culture chamber lid CCL Culture chamber interface ring CCIR Plastic ring with thread 15 mm high ID 26 mm OD 30 mm 2014 Mult Channel System MCS GmbH Product information is subject to change without notice Culture chamber CC multichannel systems Glass rings gr Plastic rings Cp Plastic rings with thread Cor 6 wellrings__ 9 wellrrings v Culture chamber 6well CC ALA MEA MEM SHEET Culture chamber 9well CC ALA MEA MEM SHEET Set of 5 culture chambers 6well CC Set o Triangle chamber ring tcr 10 mm high Plastic quadrade mq 9 mm high OD 30 mm OD 24 mm Round chamber ring rcr 10 mm high OD 30
25. 88 176 B3 61 131 225 G3 23 13 53 N6 17 43 108 G16 42 212 190 B4 58 250 198 G4 24 143 23 N7 24 173 10 H10 33 84 150 B5 51 126 162 G5 21 1 121 N8 27 48 38 H11 36 209 185 B6 48 245 134 G6 11 7 156 N9 40 181 6 H12 39 87 153 B7 41 121 232 G7 7 5 155 010 44 183 3 H13 40 211 187 B8 38 240 201 H1 32 147 21 011 47 58 31 H14 37 86 152 B9 29 115 233 H2 29 16 119 012 54 188 67 H15 38 210 188 C10 23 112 167 H3 30 146 89 013 57 63 98 H16 35 85 154 C11 20 231 139 H4 27 15 54 03 3 36 44 H9 60 221 215 C12 13 107 237 H5 28 145 24 04 10 166 80 110 34 208 186 C13 10 226 212 H6 31 17 51 05 13 41 107 111 31 83 151 c14 3 102 172 H7 34 148 90 06 20 171 9 112 28 205 59 C4 57 129 228 11 35 19 52 07 23 46 37 113 27 81 149 C5 54 248 197 12 38 150 87 08 30 176 73 114 30 206 62 C6 47 124 161 13 37 20 117 09 37 53 101 115 29 82 148 c7 44 243 133 14 40 151 19 P1 2 34 82 116 32 207 61 C8 37 119 231 15 39 21 49 P10 41 55 102 K10 7 71 63 C9 30 236 203 16 36 149 22 P11 48 185 4 K11 11 73 247 D10 24 233 140 17 33 18 120 P12 51 60 32 K12 21 78 250 D11 17 109 238 18 60 161 16 P13 58 190 68 K13 24 203 57 D12 14 228 207 K1 42 152 88 P14 61 65 95 K14 23 79 251 D4 63 132 157 K2 41 22 118 P2 1 35 112 K15 26 204 60 D5 53 127 227 K3 44 153 20 P3 6 164 79 K16 25 80 146 D6 50 246 200 K4 43 23 50 P4 9 39 110 L12 18 200 220 D7 43 122 164 K5 46 154 85 P5 16 169 12 L13 17 76 248 D8 40 241 136 K6 56 159 15 P6 19 44 40 L14 20 201 221 D9 27 114 168 L1 45 24 115 P7 26 174 76 L15 19 77 252 E10 21 111 235
26. A4 F9 F6 FA 87 ss 28 lo 7 55 A6 A F amp 7 a E8 A5 60 um F5 s 37 68 A4 A5 F11 F10 F9 F7 F5 E10 E9 E7 The letter digit code is the electrode identifier and refers to the position of the electrode in the hexa grid The specified MEA amplifier pin numbers are the channel numbers that are used in MC Rack Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice 60ThinMEA30 10iR ITO 60ThinMEA30 10iR ITO multichannel systems Technical Specifications 60ThinMEA30 10iR ITO Temperature compartibility Dimension W x D x H Thickness Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Distance between electrode fields Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm 180 um Glass part Glass on ceramic carrier ITO Indium tin oxide 10 um 30 um 500 um Planar TiN Titanium nitr
27. B75 Conn 2 No 7 C55 Conn 2 No 50 A62 Conn 1 No 29 B64 Conn 2 No 8 C46 Conn 2 No 51 A71 Conn 1 No 30 B76 Conn 2 No 9 C44 Conn 2 No 52 A63 Conn 1 No 31 B65 Conn 2 No 10 C36 Conn 2 No 53 A72 Conn 1 No 32 B66 Conn 2 No 11 C45 Conn 2 No 54 A64 Conn 1 No 33 B44 Conn 2 No 12 C26 Conn 2 No 55 A73 Conn 1 No 34 B56 Conn 2 No 13 C35 Conn 2 No 56 A54 Conn 1 No 35 B55 Conn 2 No 14 C16 Conn 2 No 57 A74 Conn 1 No 36 B46 Conn 2 No 15 C34 Conn 2 No 58 A65 Conn 1 No 37 B45 Conn 2 No 16 C15 Conn 2 No 59 A75 Conn 1 No 38 B36 Conn 2 No 17 C25 Conn 2 No 60 A86 Conn 1 No 39 B35 Conn 2 No 18 C14 Conn 2 No 61 A76 Conn 1 No 40 B26 Conn 2 No 19 C24 Conn 2 No 62 A56 Conn 1 No 41 B25 Conn 2 No 20 c33 Conn 2 No 63 A55 Conn 1 No 42 B16 Conn 2 No 21 D41 Conn 3 No 1 B24 Conn 1 No 43 B15 Conn 2 No 22 c13 Conn 3 No 2 A45 Conn 1 No 44 Electrode ID Electrode Stimulation Electrode Stimulation Electrode Stimulation and number ID Socket ID Socket ID Socket i H D41 Conn 3 No 1 E63 Conn 3 No 43 F76 Conn 4 No 23 of stimulation c13 Conn 3 No 2 D42 Conn 3 No 44 F64 Conn 4 No 24 connector socket D51 Conn 3 No 3 E53 Conn 3 No 45 F66 Conn 4 No 25 i D43 Conn 3 No 4 E73 Conn 3 No 46 F75 Conn 4 No 26 in well D E and F D61 Conn 3 No 5 E64 Conn 3 No 47 F56 Conn 4 No 27 D52 Conn 3 No 6 E74 Conn 3 No 48 F65 Conn 4 No 28 D62 Conn 3 No 7 E65 Conn 3 N
28. EDTA Collagenase Materials Collagenase Type I Sigma Aldrich Inc C0130 0 5 mM EDTA Phosphate buffered saline PBS Gibco Invitrogen 14190 144 Collagenase solution Dissolve collagenase type in PBS at 20 U ml Method 1 Fill the MEA culture chamber with 0 5 mM EDTA and incubate for 30 min 2 Rinse the chamber 3 times with PBS 3 Fill the MEA with collagenase solution and incubate for at least 30 min at 37 C 4 Discard the collagenase solution and rinse the MEA with distilled water at least 3 times 5 Air dry the MEA preferably under a laminar flow hood 44 MEA Manual 5 5 8 MEA Cleaning with Terg A Zyme Materials e Terg A Zyme Sigma Aldrich Inc 2273287 e Distilled water Terg A Zyme solution Prepare a 1 96 solution of Terg A Zyme in distilled water Method 1 2 A A Place the MEA in 1 96 Terg A Zyme solution overnight at room temperature Apply gentle shaking or rocking if possible After Terg a Zyme treatment rinse the MEA thoroughly with distilled water Terg A Zyme solution can be stored at 4 C and reused for about a week Dry the MEA and apply hydrophilic surface treatment if necessary Please see above If the MEA is going to be used for cell or tissue culture autoclave the MEA at 121 C for 30 min Do not fix cells or tissues on a MEA Detergent treatment will not remove fixed tissues Important NEVER wipe the electrode field or touch it otherwise Wa
29. Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice PMEA32S12 Layout 3 multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System Technical Specifications pMEA32S12 Layout 3 Temperature compatibility Dimension W x D x H Base material Perforation Total area of holes Diameter of the holes Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of stimulation electrodes Number of reference electrodes MC_Rack Source layout in Data Source Setup Channel map Cleaning pMEA perfusion chamber 10 50 C 49 mm x 25 mm x 1 8 mm Polyimide foil on ceramic carrier with perforation 0 8 mm 90 75 50 30 20 um Ti Titanium TiN Titanium nitride 30 um recording electrodes 50 um stimulation electrodes 90 um and 150 um recording electrodes 90 um and 100 um stimulation electrodes Planar TiN Titanium nitride electrodes Polyimide foil Approximately 30 50 kQ 1x10 1x12 71x 10 recording electrodes 3 x 4 stimulation electrodes 32 12 1 internal reference electrode Configuration MEA2100 32 1 dimensional no digital channel USB MEA32 STIM4 pMEA 3
30. Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH Fax 49 7121 9 09 25 11 info multichannelsystems com Product information is subject to change www multichannelsystems com without notice pMEA32S12 Layout 1 Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System multichannel systems Technical Specifications pMEA32S12 Layout 1 Temperature compatibility Dimension W x D x H Base material Perforation Total area of holes Diameter of the holes Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of stimulation electrodes Number of reference electrodes MC_Rack Source layout in Data Source Setup Channel map Cleaning pMEA perfusion chamber 10 50 C 49 mm x 25 mm x 1 8 mm Polyimide foil on ceramic carrier with perforation 0 8 mm 90 75 50 30 20 um Ti Titanium TiN Titanium nitride 30 um recording electrodes 50 um stimulation electrodes 90 um and 150 um recording electrodes 100 um and 125 um stimulation electrodes Planar TiN Titanium nitride electrodes Polyimide foil Approximately 30 50 kQ
31. MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm Glass ITO Indium tin oxide or Ti Titanium ITO Indium tin oxide or TIN Titanium nitride 10 or 30 um 100 or 200 um Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD 30 50 kO for 30 um electrodes 250 400 kO for 10 um electrodes 8x8 59 with iR or 60 without iR 1 internal reference electrode iR or without internal reference 2 dim MEA or Configuration Default Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2014 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice multichannel Standard 60MEA systems Standard electrode layout grid 8 x 8 E me o N MEA pins 1 dim 85 6 67 69 69 69 6 62 69 69 69 G9 9 5 y g E E o 9 2 i ig E Electrode 21 32 31 44 41 42 52 51 54 61 62 71 u 2 0 e ee 968 e 6 09 32 24 0 0 5 ner o 00 x 00r d Ez OOE E dE E E a B s ww v v se 2 a aq W q qa ul WwW 2 2 699600 88 0009060000 oe 0966060000 8 006006006060 06 oeeeeeoecc
32. Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel systems 120pMEA200 30 iR Ti 120pMEA for Use with MEA2100 120 System Layout with 12x 12 electrode grid 2 ps p P es er eg m as P aa Bea H na Yia T u P 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 7317475 OW B6 34 87 ke nm 9 gt E Es 32 z 89 ms or s 90 Hs E7 396 gt _ G7 r 29 3 gt 9 57 28 oe N Sd 93 m7 c7 27 94 17 o7 126 S 4 955 K7 as 25 96 37 Bs E24 ee 97 ws ce 23 e 98 is pe 22 X 99 Ke as 21 100 ve B 20 101 ws co 19 402 Ls es Mg L 103 Ko B1017 404 ve c 105 L1 i ifi i i 1 1514 1312 11 432 nanzu mari 1101 Technical Specifications 120pMEA200 30iR Ti on ke len heal pl eel Cii D10 Di2 E10 E12 F10 F12 F7 G8 G11 G9 Hit H9 J11 H8 K10 Temperature compatibility 0 50 C Dimension W x D x H 49 x 49x 1 mm Base material Polyimide foil 2611 with perforation on glass carrier Perforation Diameter of innermost area 3 4mm Total area of holes 9 12 according to 3 4 mm Diameter of the holes 15 20 25 30 40 45 50 um Contact pads TiAu Titan Gold Track material TiAuTi Titan Gold Titan Electrode diameter 30 u
33. a better contact with the surrounding tissue The electrodes are from titanium nitride TiN contact pads and track material from pure gold FlexMEAs are stable at a temperature range from 10 C to 125 C and can be autoclaved Using FlexMEAs Warning Do not use an ultrasonic bath for cleaning The manufacturer recommends sterilization by rinsing with alcohol FlexMEAs are usually connected to a head stage preamplifier that is connected to a filter amplifier or programmable gain amplifier see also the ME System product line of Multi Channel Systems Via provided adapters FlexMEAs can be connected to 32 channel miniature preamplifiers MPA32I from Multi Channel Systems for in vivo experiments There is no need for an adapter if the FlexMEA should be connected to the 32 channel miniature preamplifier MPA32I Flex 30 MEA Manual FlexMEA36 The FlexMEA36 has 32 recording electrodes plus two internal reference electrodes and two ground electrodes in a 6 x 6 electrodes grid The titanium nitride electrodes have a diameter of 30 um and the distance between the electrodes is 300 um The polyimide foil is perforated with holes of 30 um diameter ensuring optimal tissue contact When using the Flex MEA36 together with a standard 32 channel miniature preamplifier MPA32I you need the ADPT FM 32 adapter to connect the FlexMEA36 to the standard MPA3Z2I There is no need for an adapter if you use the FlexMEA36 specified 32 channel miniature
34. chambers and rings with round chambers The electrodes of the 60 6wellMEA are from titanium nitride TiN the isolation is made up of Silicon nitride SIN Contact pads are from titanium nitride TiN and tracks are from titanium Ti The diameter of the electrodes is 30 um the distance from centre to centre is 200 um Using 60 6wellMEAs 60 6wellMEAs can be used for a wide variety of applications They are robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Generally they can be used for acute experiments as well as long term cultures 22 MEA Manual 4 11256MEA for Use with USB MEA256 System 256MEA30 8 ITO 256MEA60 10iR ITO 256MEA100 30 ITO 256MEA200 30 ITO and 256ThinMEA The 256MEAs have to be used with the USB MEA256 System Please refer to the USB MEA256 System manual for detailed information The 256MEA contains 252 recording and four ground electrodes arranged in a 16 x 16 layout grid embedded in a transparent glass substrate The contact to the amplifier is provided by a double ring of contact pads around the rim of the MEA The standard material for MEAs is also used for 256MEAs The electrodes are from titanium nitride TiN with a silicon nitride SiN isolator and contact pads and tracks are made of transparent indium tin oxide ITO The spacing of the electrodes in the 16 x16 grid averages 30 60 100 um or 200 um between the e
35. channels the MEA2100 HS 120 mA XA ee e o c o 000o XI ee 94 Buuousscn STET Te zr e nem TA rr Honnu rntona ey ty pam ann a LAIIILIT ES 0 0 0 0 000000000 9 9999999990000 066 O e werire XC Y Oe Felt ILLI T LJ LESTIE LETT pee yrtrrriy i i 256MEA SG 120MEA SG For FlexMEAs connected to ME Systems and for wireless headstages connected to the Wireless System you can use a specially adapted signal generator ME W SG 34 MEA Manual 5 MEA Handling A A A Warning If possible use only liquids or cleaning solutions with a neutral pH 7 on MEAs Do not expose MEAs with a silicon nitride insulation or TiN electrodes to basic liquids pH gt 7 or aggressive detergents for a longer period of time Basic or aggressive liquids may damage TiN electrodes irreversibly Warning It is absolutely necessary to rinse the MEAs thoroughly with distilled water after treatment with detergent particular when using Terg A Zym before heat sterilization dry heat sterilization is not recommended Otherwise the potential rests of the detergent may burn into the glass carrier of the MEA and may destroy the electrodes Warning Do not touch the electrode field in any way during the coating or cleaning procedure Keep all instruments tissues pipette tips and similar at a safe distance from the recording area The electrodes are easily damaged except EcoMEA electrodes 5 1 Hy
36. grid with electrode diameters of 10 um or 30 um and interelectrode distances of 100 um 200 um The MEAs with an interelectrode distance of 500 um have a 6 x 10 layout grid Versions 200 10 200 30 100 10 are available without or with an internal reference electrode as indicated by the abbreviation iR You can connect the internal reference electrode directly to the amplifier s ground and will not need silver pellets for grounding the bath anymore Please refer to the MEA manual delivered with your MEA amplifier for more information The flat round electrodes are made of titanium nitride TIN MEAs with TiN electrodes are very stable Therefore the MEAs can be reused several times and are perfect for long time experiments up to several weeks and even months The electrode impedance ranges between 30 kO and 400 kO depending on the electrode diameter Generally the smaller the electrode the higher is the impedance Tracks are made of titanium Ti and contact pads are made of titanium nitride TiN or indium tin oxide ITO insulation material is silicon nitride ITO contact pads and tracks are transparent for a perfect view of the specimen under the microscope Important MEAs are not symmetrical That is why the writing for example NMI LEITER MEA type on the MEA chip should be on the right side viewed from the front with the sockets of the amplifier in the back MEAs with one big internal reference electrode should be placed with ref
37. not use an ultrasonic bath for cleaning 20 MEA Manual 4 9 Perforated MEAs for Use with MEA2100 32 System and USB MEA32 STIM4 System pMEA 32512 L1 pMEA 32512 L2 pMEA 32S12 L3 pMEA 32512 L4 pMEA 32S12 Lx For the USB MEA32 STIM4 System small perforated MEAs have been designed Please see USB MEA32 STIM4 manual for detailed information Additionally the MEA2100 32 System is adapted for these small type of MEA The pMEAs are different in size but identical in function to the regular pMEAs Layout 1 pMEA 32S12 L1 of the perforated MEAs designed for the MEA2100 32 and for the USB MEA32 STIM4 System has been optimized for acute hippocampal slices The flat round electrodes are made of titanium nitride TiN with a diameter of 30 um for the recording electrodes and 50 um for the stimulation electrodes The stimulation electrodes can not be used for recording and vice versa The interelectrode distances vary from 150 to 200 um MEAs with titanium nitride TiN electrodes are very stable Therefore the pMEA can be reused several times and is perfect for long time experiments up to several weeks and even months The electrode impedance ranges between 30 kO and 50 kQ Tracks and contact pads are made of titanium nitride TiN the insulation material is polyimide respectively The electrodes are integrated into a thin polyimide foil This thin foil is fixed on a ceramic waver for mechanical stability In the middle of the waver und
38. or even fatal injuries Warning Obey always the rules of local regulations and laws Only qualified personnel should be allowed to perform laboratory work Work according to good laboratory practice to obtain best results and to minimize risks The product has been built to the state of the art and in accordance with recognized safety engineering rules The device may only be used for its intended purpose be used when in a perfect condition Improper use could lead to serious even fatal injuries to the user or third parties and damage to the device itself or other material damage Warning The device and the software are not intended for medical uses and must not be used on humans Malfunctions which could impair safety should be rectified immediately Regard the technical specifications of the various MEA types especially the temperature range and the safe charge injection limits for stimulation Do not autoclave or expose pMEAs to heat more than 50 C Do not touch the electrode field in any way Do not use any liquids or cleaning solutions with a high pH 7 for a longer period of time on MEAs of a silicon nitride insulation type Basic solutions will damage TiN electrodes MEA Manual 3 Microelectrode Arrays MEAs Overview 3 1 Extracellular Recording with Microelectrode Arrays A microelectrode array MEA is an arrangement of typically 60 electrodes allowing the targeting of several sites in parallel for extracel
39. subject to change Germany www multichannelsystems com without notice pMEA32S12 Layout 4 multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System Technical Specifications pMEA32S12 Layout 4 Temperature compatibility Dimension W x D x H Base material Perforation Total area of holes Diameter of the holes Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of stimulation electrodes Number of reference electrodes MC_Rack Source layout in Data Source Setup Channel map Cleaning A pMEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 10 50 C 49 mm x 25 mm x 1 8 mm Polyimide foil on ceramic carrier with perforation 0 8 mm 90 75 50 30 20 um Ti Titanium TIN Titanium nitride 30 um recording electrodes 50 um stimulation electrodes 100 um and 100 um recording electrodes 100 um and 100 um stimulation electrodes Planar TiN Titanium nitride electrodes Polyimide foil Approximately 30 50 kQ 4 x 8 recording electrodes 2x6 stimulation electrodes 32 12 1 internal reference electrode Configuration MEA2100 32 1 dimensional no digital channel USB MEA32 STIM4 pMEA 32812 L4 8x4 cmp Rinse with distilled w
40. the impedance and the noise level down has been met by introducing a new electrode material Titanium nitride TiN The NMI in Reutlingen Germany www nmi de produces MEAs from very pure fine quality and highly biocompatible materials The NMI is a research institute with which Multi Channel Systems has collaborated in many projects and over many years Quality controls and production processes have been improved over the last years so that MEAs are always of a fine consistent quality at very reasonable prices 10 MEA Manual 3 3 Electrodes Tracks and Insulation Microfold structures result in a large surface area that allows the formation of electrodes with an excellent signal to noise ratio without compromising on the spatial resolution TiN titanium nitride is a very stable material that for example is also widely used for coating heavy equipment All MEAs with TiN electrodes have a long life and can be reused several times if handled with care If used for acute slices MEAs can be used for approximately one year Additionally available are EcoMEAs equipped with gold Au electrodes Long time experiments with cell cultures and rigid cleaning methods shorten the MEA lifetime but you can still reuse a MEA about 30 times depending on the coating cell culture and cleaning procedure All MEAs except pMEAs show excellent temperature compatibility and are stable from 0 C to 125 C that is they can be autoclaved The
41. the product In most cases it is only a minor problem that can be easily avoided or solved If the problem persists please contact your local retailer The highly qualified staff will be glad to help you Please inform your local retailer as well if other problems that are not mentioned in this documentation occur even if you have solved the problem on your own This helps other users and it helps Multi Channel Systems to optimize the instrument and the documentation Please pay attention to the safety and service information chapter Important Information and Instructions Multi Channel Systems has put all effort into making the product fully stable and reliable but like all high performance products it has to be handled with care 9 2 Technical Support Please read the Troubleshooting part of the user manual first Most problems are caused by minor handling errors Contact your local retailer immediately if the cause of trouble remains unclear Please understand that information on your hardware and software configuration is necessary to analyze and finally solve the problem you encounter Please keep information on the following at hand e Description of the error the error message text or any other useful information and of the context in which the error occurred Try to remember all steps you had performed immediately before the error occurred The more information on the actual situation you can provide the easier it is to tra
42. with Polyethyleneimine PEI plus Laminin 5 4 3 Coating with Polyornithine plus Laminin 5 4 4 Coating with Poly D Lysine plus Laminin 5 4 5 Coating with Poly D Lysine plus Fibronectin 5 4 6 Coating with Fibronectin 5 4 7 Coating with Collagen Cleaning of used MEAs 5 5 1 General Recommendations for Cleaning MEAs 5 5 2 Cleaning of pMEAs 5 5 3 Cleaning of ECOMEAs 5 5 4 Cleaning of EcoFlexMEAs 5 5 5 Cleaning of FlexMEAs 5 5 6 Removing Nitrocellulose Coating 5 5 7 MEA Cleaning with EDTA Collagenase 5 5 8 MEA Cleaning with Terg A Zyme Culture Chamber Options MEA2100 CO2 C Sealed MEA Culture Dish MEA Culture Chamber with Lid Culture Chamber for 9 Well MEAs Culture Chamber for 6 Well MEAs 35 35 35 35 36 36 36 36 36 36 37 37 37 38 39 40 41 41 42 43 43 43 43 43 44 44 44 45 47 47 47 48 48 48 Microelectrode Arrays MEAs Overview 7 Recording with MEAs 50 7 1 Mounting the MEA 50 7 1 1 Cleaning the Contact Pads 50 7 1 2 Positioning the MEA 50 7 1 3 Grounding the Bath 50 7 2 General Performance Noise Level 51 8 Stimulation 53 8 1 Using MEA Electrodes for Stimulation 53 8 2 Capacitive Behavior of Stimulating Electrodes 54 8 3 Aspects of Electrode Size and Material 55 8 4 Recommended Stimulus Amplitudes and Durations 56 9 Troubleshooting 57 9 1 About Troubleshooting 57 9 2 Technical Support 57 9 3 Noise on Single Electrodes 58 9 4 Overall Noise Unsteady Baseline 60 9 5 Missing Spike
43. with the recording and electrodes deteriorate over time due to electrolysis You can avoid that by choosing an appropriate stimulus protocol that actively discharges the electrode after the pulse When using voltage driven stimulation the electrodes are discharged when the voltage level is set to zero at the end of the monophasic pulse Not so in current mode When applying a negative current pulse the electrode is charged and needs to be actively discharged by applying an inverted pulse with a matching product of current and time that is you need to stimulate with biphasic pulses for current driven stimulation to reduce both the stimulus artifact and to avoid an electrode damage The easiest way is to use the same signal amplitude and the same duration with an inverse polarity For voltage driven stimulation monophasic pulses are fine The following illustration shows the effect of a biphasic current pulse on the discharge of the stimulating electrode As you can see the first monophasic pulse is followed immediately by a pulse of the opposite polarity and the same product of current and time Effect of a bipolar pulse on the electrode voltage Monopolar pulse Bipolar pulse l U l U Electrode Electrode voltage voltage Stimulus Stimulus 54 Stimulation 8 3 Aspects of Electrode Size and Material Titanium nitrite TiN electrodes are generally more robust than electrodes from other materials for example platinum Pt In the Ap
44. 0 198 59 E51 31 46 59 E64 64 30 59 E25 97 183 59 E12 130 160 60 E42 161 16 60 E53 191 125 60 E34 221 215 60 E23 251 129 61 E62 32 111 61 E55 65 95 61 E14 98 239 61 E21 131 225 62 E52 162 81 62 E54 192 e5 62 E24 222 209 62 E22 252 195 63 E43 33 43 63 E44 66 27 63 E33 99 171 63 E32 132 157 GND GND GND GND 9 Well MEA for use with USB MEA256 System Sti Socket Stimulation socket number in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier connecting to the contact pads of the 256 9wellMEA Hardware ID Hardware channel ID of MC_Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 9 x 28 layout grid Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 120MEA 120MEA200 30iR Ti 120MEA100 30iR ITO multichannel systems 120MEA for Use with MEA2100 120 System with 12 x 12 electrode grid o gt 7M SS FNS Technical Specifications 120MEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height
45. 0 6wellMEA Important Please insert the 60 6wellMEA into the MEA amplifier with the writing on the A MEA chip in this example MCS on the left side viewed from the front with the sockets of the MEA1060 amplifier or the articulation of the MEA2100 headstage in the back w bs Z7 QZ 2013 Multi Channel Systems MCS GmbH multichannel systems 60 6wellMEA MC Rack Electrode Layout Channel Map Please make sure to select in MC_Rack program Data Source Setup Source layout 2 dim MEA digit code or Configuration letter digit code Fl F2 F3 A1 A2 A3 B1 B2 B3 The letter digit code is 34 23 22 43 42 51 63 82 83 the electrode identifier and refers to the position F4 F5 F6 A4 A5 A6 B4 B5 B6 of the electrode 24 12 33 44 52 53 71 73 64 in the 60 6wellMEA F7 F8 F9 A7 A8 A9 B7 B8 B9 The amplifier electrode 14 13 21 31 41 54 62 72 74 numbers digit are in same sequence as they E9 E8 E7 D9 D8 D7 C9 C8 C7 are in the data display 25 27 37 45 58 68 78 86 85 of MC_Rack E6 E5 E4 D6 D5 D4 c6 C5 C4 35 26 28 46 47 55 66 87 75 Channel map ES E2 El D3 D2 D1 CS C2 C1 6 Well MEA cmp 16 17 36 48 57 56 77 76 65 2012 Multi Channel Systems MCS GmbH 256MEA 256MEA30 8iR ITO 256MEA60 10iR ITO 256MEA100 30iR ITO 256MEA200 30iR ITO N v 256 Microelectrode Array for use with USB MEA256 System NS BEEEN ESEREREEESESEBSEREESENGSESTSRNRRS BEEENEEBSSRSESSESSESEESSSARSSSSEENEESEN multi
46. 1 62 C24 192 65 62 E26 222 209 62 F63 252 195 63 B71 33 43 63 c33 66 27 63 E16 99 171 63 F54 132 157 GND GND GND GND 256 6wellMEA for use with USB MEA256 System Stim Socket Stimulation socket number in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier connecting to the contact pads of the 256 6wellMEA Hardware ID Hardware channel ID of MC Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 6 x 6 x 7 layout grid Multi Channel Systems Fon 49 7121 9 09 25 O MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Germany www multichannelsystems com 2014 Multi Channel Systems MCS GmbH Product information is subject to change without notice multichannel 256 6wellMEA systems Tables Electrode Stimulation Electrode Stimulation Electrode Stimulation ID Socket I D Socket ID Socket A46 Conn 1 No 1 B24 Conn 1 No 43 c11 Conn 2 No 23 Electrode ID F74 Conn 1 No 2 A45 Conn 1 No 44 C23 Conn 2 No 24 and number A36 Conn 1
47. 12 F61 Conn 4 No 55 D14 Conn 4 No 34 E31 Conn 4 No 13 F52 Conn 4 No 56 D33 Conn 4 No 35 E32 Conn 4 No 14 F71 Conn 4 No 57 D13 Conn 4 No 36 E41 Conn 4 No 15 F53 Conn 4 No 58 D22 Conn 4 No 37 E42 Conn 4 No 16 F72 Conn 4 No 59 D12 Conn 4 No 38 E51 Conn 4 No 17 F62 Conn 4 No 60 D21 Conn 4 No 39 E52 Conn 4 No 18 F73 Conn 4 No 61 D11 Conn 4 No 40 E61 Conn 4 No 19 F63 Conn 4 No 62 D31 Conn 4 No 41 E62 Conn 4 No 20 F54 Conn 4 No 63 D32 Conn 4 No 42 E71 Conn 4 No 21 A46 Conn 1 No 1 E63 Conn 4 No 43 E72 Conn 4 No 22 F74 Conn 1 No 2 D42 Conn 4 No 44 Multi Channel Systems Fon 49 7121 9 09 25 O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 256 9wellMEA 256 9wellMEA300 30iR ITO w o 256 9wellMEA300 30iR ITO mq multichannel systems 9 well Microelectrode Array for use 2 with USB MEA256 System L1 t o m o o c c o O QUOI mi ea ol Bn Z 10 08UU0D LI NOOO O ILICILILIDDOICICILI EIC IDIDCI I ILICE TE IEDIDOCICICICIDICI The MEA is not symmetrical and has to be inserted into the amplifier with the writing Xe Connector 3 d MCS on top as shown in the pictu
48. 142 91 22 B15 172 75 22 D56 202 58 22 E72 232 205 23 A43 13 53 23 C11 46 37 23 D44 79 251 23 F76 112 167 24 A41 143 23 24 C23 173 10 24 D46 203 57 24 F64 233 140 25 A52 14 122 25 c21 47 106 25 D35 80 146 25 F66 113 236 26 A51 144 92 26 C12 174 76 26 D36 204 60 26 F75 234 206 27 A53 15 54 27 c31 48 38 27 D34 81 149 27 F56 114 168 28 A61 145 24 28 C22 175 7 28 D26 205 59 28 F65 235 137 29 A62 16 119 29 c41 49 103 29 D25 82 148 29 F46 115 233 30 A71 146 89 30 C32 176 73 30 D16 206 62 30 F55 236 203 31 A63 17 51 31 c51 50 35 31 D24 83 151 31 F36 116 165 32 A72 147 21 32 C42 177 8 32 D15 207 61 32 F45 237 138 33 A64 18 120 33 C61 51 104 33 D23 84 150 33 F26 117 234 34 A73 148 90 34 C52 178 74 34 D14 208 186 34 F35 238 204 35 A54 19 52 35 c71 52 36 35 D33 85 154 35 F16 118 166 36 A74 149 22 36 C43 179 5 36 D13 209 185 36 F44 239 135 37 A65 20 117 37 C72 53 101 37 D22 86 152 37 F15 119 231 38 A75 150 87 38 C62 180 71 38 D12 210 188 38 F25 240 201 39 A66 21 49 39 C73 54 33 39 D21 87 153 39 F14 120 163 40 A76 151 19 40 c63 181 6 40 D11 211 187 40 F24 241 136 41 A56 22 118 41 C74 55 102 41 D31 88 176 41 F13 121 232 42 A55 152 88 42 C53 182 72 42 D32 212 190 42 F34 242 202
49. 1x10 1x12 1 x 10 recording electrodes 2x6 stimulation electrodes 32 12 1 internal reference electrode Configuration MEA2100 32 1 dimensional no digital channel USB MEA32 STIM4 pMEA 32812 L1 12x3 cmp Rinse with distilled water Do not use ultrasonic bath Do not autoclave or sterilize pMEAs by heat These MEA types are not heat stable and will be irreversibly damaged w o Without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www mu Itichannelsystems com without notice multichannel pMEA32S12 Layout 1 Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System egg I III Oo OOO CH19 CH29 E G CH16 fi E CH13 9 CH12 S CH11 8 CH14 2 CH15 a CH10 CH 8 CH 6 CH 4 The oval area of the pMEA chip is perforated A4 AT A10 A13 A16 A19 A22 A25 A28 A31 L 150 um 90 um A1 A 3 A6 A9 A12 A15 A18 A21 A24 A27 A30 A32 A 4 A5 A8 A11 A14 A17 A20 A23 A26 A29 30 um 100 um D recording electrode 50 um amp stimulation electrode 125 um Electrode layout in the grid Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 2013 Multi Channel Systems M
50. 2 C12 167 11 12 J64 197 219 12 G64 227 143 13 A32 8 123 13 C32 41 107 13 J44 74 246 13 G44 107 237 14 A21 138 93 14 C21 168 77 14 J55 198 218 14 G55 228 207 15 A41 9 55 15 c41 42 39 15 J35 75 249 15 G35 108 169 16 A31 139 25 16 C31 169 12 16 J45 199 222 16 G45 229 144 17 A51 10 124 17 C51 43 108 17 J25 76 248 17 G25 109 238 18 A42 140 94 18 C42 170 78 18 J34 200 220 18 G34 230 208 19 A62 11 56 19 ce2 44 40 19 J14 77 252 19 G14 110 170 20 A52 141 26 20 C52 171 9 20 J24 201 221 20 G24 231 139 21 A63 12 121 21 c63 45 105 21 J13 78 250 21 G13 111 235 22 A43 142 91 22 c53 172 75 22 J33 202 58 22 G23 232 205 23 A53 13 53 23 c43 46 37 23 J23 79 251 23 G33 112 167 24 AS2 143 23 24 CS2 173 10 24 JS1 203 57 24 GS1 233 140 25 A54 14 122 25 C54 47 106 25 J22 80 146 25 G22 113 236 26 A64 144 92 26 Ce4 174 76 26 J12 204 60 26 G12 234 206 27 A44 15 54 27 c44 48 38 27 J32 81 149 27 G32 114 168 28 A55 145 24 28 c55 175 7 28 J21 205 59 28 G21 235 137 29 B35 16 119 29 F41 49 103 29 H41 82 148 29 D35 115 233 30 A45 146 89 30 c45 176 73 30 J31 206 62 30 G31 236 203 31 B25 17 51 31 F51 50 35 31 H51 83 151 31 D25 116 165 32 B34 147 21 32 F42 177 8 32 H42 207 61 32 D34 237 138 33 B14 18 120 33 F62 51 104 33 H62 84 150 33 D14 117 234 34 B24 148 90 34 F52 178 74 34 H52 208 186 34 D24 238 204 35 B33 19 52 35 FS2 52 36 35 H43 85 15
51. 200 150 100 50 500 1000 1500 2000 2500 3000 time us safe charge injection limits 0 1 1 1 L 1 3000 4000 5000 6000 7000 8000 9000 10000 time us Safe Charge Injection Limits of Micro Electrode Arrays with TiN Electrodes diameter 10 um max pulse amplitude WA max pulse amplitude WA max pulse amplitude uA safe charge injection limits 2000 T T r 1500 1000 500 0 5 10 15 20 25 30 35 40 45 50 time us safe charge injection limits 400 350 300 250 200 150 100 50 r 0 1 L L 1 50 100 150 200 250 300 time us safe charge injection lim its 60 50 40 30 20 10 300 400 500 600 700 800 900 1000 time us Appendix 10 3 Data Sheets 65 Standard 60MEA multichannel systems 60MEA200 10IiR ITO 60MEA200 30iR ITO 60MEA100 1 0iR ITO 60MEA100 10 ITO 60MEA100 1 0IR Ti 60MEA200 30 Ti 60MEA200 10IR Ti 60MEA200 30IR Ti 49 0 mm Contact pads 49 0 mm Standard electrode layout grid 8 x 8 T Technical Specifications Standard 60MEA Temperature compatibility Dimension W x D x H Base material Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map
52. 22 P11 48 185 4 K11 11 73 247 D10 24 233 140 17 33 18 120 P12 51 60 32 K12 21 78 250 D11 17 109 238 18 60 161 16 P13 58 190 68 K13 24 203 57 D12 14 228 207 K1 42 152 88 P14 61 65 95 K14 23 79 251 D4 63 132 157 K2 41 22 118 P2 1 35 112 K15 26 204 60 D5 53 127 227 K3 44 153 20 P3 6 164 79 K16 25 80 146 D6 50 246 200 K4 43 23 50 P4 9 39 110 L12 18 200 220 D7 43 122 164 K5 46 154 85 P5 16 169 12 L13 17 76 248 D8 40 241 136 K6 56 159 15 P6 19 44 40 L14 20 201 221 D9 27 114 168 L1 45 24 115 P7 26 174 76 L15 19 77 252 E10 21 111 235 L2 48 155 17 P8 29 49 103 L16 22 202 58 E11 18 230 208 L3 47 25 47 P9 38 180 71 M12 8 195 217 E12 8 225 142 L4 50 156 86 R10 42 182 72 M13 14 198 218 E6 49 125 230 L5 49 26 116 R11 45 57 99 M14 13 74 246 E7 46 244 199 L6 59 31 46 R12 52 187 1 M15 16 199 222 E8 39 120 163 M1 52 157 18 R13 55 62 29 M16 15 75 249 E9 28 235 137 M2 51 27 48 R14 62 192 65 N14 10 196 216 F10 11 106 174 M3 54 158 83 R2 4 163 13 N15 9 72 244 F6 59 130 160 M4 53 28 113 R3 5 37 109 N16 12 197 219 F7 56 249 132 N1 55 29 45 R4 12 167 11 014 3 69 28 F8 36 239 135 N2 58 160 84 R5 15 42 39 015 6 194 214 F9 31 116 165 N3 57 30 114 R6 22 172 75 016 5 70 64 G10 7 104 173 N4 63 33 43 R7 25 47 106 P15 1 68 96 G8 33 117 234 01 62 162 81 R8 32 177 8 P16 4 193 126 G9 34 238 204 02 61 32 111 R9 35 52 36 R15 2 67 66 H8 60 251 129 GND 64 253 GND 64 254 GND 64 255 GND 64 256 Stim Socket Stimulation socket numbe
53. 23 D2 12 22 43 52 53 72 82 D6 3 63 13 D1 13 23 41 42 51 73 83 D7 83 HELM 500 Ga Ee z Q e E6 64 6 n nd 7 6 14 G4 E F4 84 84 E Qe 6 La 9 NC 85 amp 6 Q RS 0 amp 9 9 26 K2 16 26 48 57 58 76 86 K6 76 Qu 96029 9609 v amp Go 0020 e o i 9 0 09 09 37 38 68 67 Mi M2 O2 O3 M3 LB KR K4 L M5 05 06 M6 M7 The letter digit code is the electrode identifier and refers to the position of the electrode in the four quadrant grid The specified MEA amplifier pin numbers are the channel numbers that are used in MC_Rack when using the 2 dimensional layout in Data Source Setup Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel k 60SquareMEA200 50IR Ti systems Microelectrode Array with 60 square electrodes E s y ee pj ra 22 Ba Be B2 ez zz a rs 23 Bs 3 Ba es 73 as 25 ss as 5s es rs ss zs Ee as Be be Ze se v aaa Ez 57 m 7 Technical Specifications 608quareMEA200 50iR Ti Temperature compatibility Dimension W x D x H Base material Track material Contact pads Electrode square size Interelectrode distance centre to centre E
54. 2812 L3 12x3 cmp Rinse with distilled water Do not use ultrasonic bath Do not autoclave or sterilize pMEAs by heat These MEA types are not heat stable and will be irreversibly damaged w o Without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice PMEA32S12 Layout 3 Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System CH17 CH19 CH29 CH16 CH13 CH12 CH11 CH14 CH15 Direction to Amplifier CH10 CH 8 CH 6 CH 4 The oval area of the pMEA chip is perforated 90 um 1 A4 A7 A10 150 um A 1 A3 A6 A9 9 A5 A8 30 um A13 A12 A11 Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany A16 A19 A15 A18 A14 A17 A20 A23 A Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com multichannel CH18 CH21 CH 2 CH 9 A26 recording electrode CH20 CH 7 systems ON 1 CN N N EEE i REF OU U EE CH26 CH31 CH32 CH24 CH27 CH28 CH30 STG24 STG26 STG13 STG12 STG11 STG14 SMETTIEE 5 09905 5H Sh b 90 um o e dp A30 A32 02 stimulation electrode nn e T 3 2013 Multi Ch
55. 3 000sec Xs 1000 ms gt oo HH 1400 366th sweep Y Asis 20 p WV 200 4l3hsweep YAsis 20 uV 200 200 1000 100 ETE ri sali i p 1090 10 20 30 40 50 30 mn onm i nuum un L L 1 1 eo 70o 80 100 110 120 130 J o dt tt 1 NN VN ET AA A SUIT RU EO S NARRANT AMANTI 60 Troubleshooting The next screen shot shows the effect of shielding The noise level is neglectible and the baseline is steady The shielding has been achieved with a metal plate connected to the metal part of the 68 pin MCS high grade cable connector and placed above the amplifier You could also use aluminum foil or a Faraday cage for the same effect for example imi Standard Noise with Shielding COC 514000sec is 1000 ms gt oo H 5000 Sl hsweep YAsis 20 W 200 200 50 100 150 200 250 300 0 La iil Li Lia iti 350 400 45i 1 nen silis 9 5 Missing Spikes or Strange Signal Behavior MEAs wear out after multiple uses or over a longer time of use for example for long term cultures The insulation layer gets thin over time This is considered a normal behavior Possible causes The insulation layer is too thin As a result the MEA gets the behavior of a low pass filter This means that the signal frequency may be shifted to a lower frequency and spikes are missing
56. 34 Conn 1 No 4 B34 Conn 1 No 32 c34 Conn 2 No 4 A35 Conn 1 No 1 B35 Conn 1 No 29 c35 Conn 2 No 1 A41 Conn 1 No 15 B41 Conn 1 No 43 c41 Conn 2 No 15 A42 Conn 1 No 18 B42 Conn 1 No 46 ca2 Conn 2 No 18 A43 Conn 1 No 22 B43 Conn 1 No 50 c43 Conn 2 No 23 A44 Conn 1 No 27 B44 Conn 1 No 55 c44 Conn 2 No 27 A45 Conn 1 No 30 B45 Conn 1 No 58 C45 Conn 2 No 30 A51 Conn 1 No 17 B51 Conn 1 No 45 C51 Conn 2 No 17 A52 Conn 1 No 20 B52 Conn 1 No 48 c52 Conn 2 No 20 A53 Conn 1 No 23 B53 Conn 1 No 51 C53 Conn 2 No 22 A54 Conn 1 No 25 B54 Conn 1 No 53 C54 Conn 2 No 25 A55 Conn 1 No 28 B55 Conn 1 No 56 C55 Conn 2 No 28 A62 Conn 1 No 19 Be2 Conn 1 No 47 c62 Conn 2 No 19 A63 Conn 1 No 21 B63 Conn 1 No 49 c63 Conn 2 No 21 A64 Conn 1 No 26 B64 Conn 1 No 54 c64 Conn 2 No 26 AS2 Conn 1 No 24 BS2 Conn 1 No 52 cs2 Conn 2 No 24 Electrode ID Electrode Stimulation Stimulation Electrode Stimulation and number aD Soc ID Socket ID Socket i i DS1 Conn 4 No 35 ES1 Conn 4 No 2 FS1 Conn 2 No 51 of sti mulation D12 Conn 4 No 40 E12 Conn 4 No 59 F12 Conn 2 No 54 connector socket D13 Conn 4 No 38 E13 Conn 4 No 57 F13 Conn 2 No 49 D14 Conn 4 No 33 E14 Conn 3 No 61 F14 Conn 2 No 47 in well D E and F D21 Conn 4 No 42 E21 Conn 4 No 61 F21 Conn 2 No 56 D22 Conn 4 No 39 E22 Conn 4 No 62 F22 Conn 2 No 53 D23 Conn 4 No 37 E23 Conn 4 No 60 F23 Con
57. 4 19 4 20 Table of Contents Introduction About this Manual Important Information and Instructions Operator s Obligations Guarantee and Liability Important Safety Advice Microelectrode Arrays MEAs Overview Extracellular Recording with Microelectrode Arrays MEA Design and Production Electrodes Tracks and Insulation MEA Types and Layouts Standard Electrode Numbering Standard MEA High Density MEA GOHDMEA Hexa MEA 60HexaMEA Thin MEA 60ThinMEA Eco MEA 60ECOMEA Stimulation MEA 60StimMEA Perforated MEA 60pMEA Perforated MEAs for Use with MEA2100 32 System and USB MEA32 STIM4 System MEA with 6 Wells 60 6wellMEA 256MEA for Use with USB MEA256 System MEA with 6 Wells for Use with USB MEA256 System MEA with 9 Wells for Use with USB MEA256 System 120MEA with for Use with MEA2100 120 System Quadrant MEA 60 4QMEA 1000 Square MEA 60SquareMEA PEDOT CNT MEAs 60PedotMEA FlexMEA EcoFlexMEA MEA Signal Generator 6G0OMEA SG Oo oN N N 10 11 12 13 14 15 16 17 18 19 20 MEA Manual 5 2 5 3 5 4 5 5 6 1 6 2 6 3 6 4 6 5 MEA Handling Hydrophilic Surface Treatment 5 1 1 Plasma Cleaning 5 1 2 Protein Coating 5 1 3 Preculturing Sterilization 5 2 1 Sterilization with Ethanol and UV Light 5 2 2 Steam Sterilization Autoclavation 5 2 3 Dry Heat Sterilization 5 2 4 Sterilization with Hot Water MEA Storage MEA Coating 5 4 1 Coating with Nitrocellulose 5 4 2 Coating
58. 4 35 DS1 118 166 36 BS1 149 22 36 F43 179 5 36 HS2 209 185 36 D33 239 135 37 B23 20 117 37 F53 53 101 37 H53 86 152 37 D23 119 231 38 B13 150 87 38 F63 180 71 38 H63 210 188 38 D13 240 201 39 B22 21 49 39 F54 54 33 39 H54 87 153 39 D22 120 163 40 B12 151 19 40 F64 181 6 40 H64 211 187 40 D12 241 136 41 B32 22 118 41 F44 55 102 41 H44 88 176 41 D32 121 232 42 B21 152 88 42 F55 182 72 42 H55 212 190 42 D21 242 202 43 B41 23 50 43 F35 56 34 43 H35 89 175 43 D41 122 164 44 B31 153 20 44 F45 183 3 44 H45 213 189 44 D31 243 133 45 B51 24 115 45 F25 57 99 45 H25 90 178 45 D51 123 229 46 B42 154 85 46 F34 184 69 46 H34 214 192 46 D42 244 199 47 B62 25 47 47 F14 58 31 47 H14 91 177 47 D62 124 161 48 B52 155 17 48 F24 185 4 48 H24 215 191 48 D52 245 134 49 B63 26 116 49 F13 59 100 49 H13 92 180 49 D63 125 230 50 B43 156 86 50 F33 186 70 50 H33 216 145 50 D43 246 200 51 B53 27 48 51 FS1 60 32 51 H23 93 179 51 DS2 126 162 52 BS2 157 18 52 F23 187 1 52 HS1 217 147 52 D53 247 162 53 B54 28 113 53 F22 61 97 53 H22 94 182 53 D54 127 227 54 B64 158 83 54 F12 188 67 54 H12 218 243 54 D64 248 197 55 B44 29 45 55 F32 62 29 55 H32 95 181 55 D44 128 159 56 B55 159 15 56 F21 189 2 56 H21 219 245 56 D55 249 132 57 E41 30 114 57 E63 63 98 57 E35 96 184 57 E13 129 228 58 B45 160 84 58 F31 190 68 58 H31 220 213 58 D45 25
59. 42 202 E3 13 8 123 M5 8 165 14 F12 49 92 180 A8 35 118 166 E4 14 138 93 M6 18 170 78 F13 50 216 145 A9 32 237 138 E5 8 135 253 M7 21 45 105 F14 47 91 177 B10 26 234 206 F1 22 142 91 M8 28 175 7 F15 48 215 191 B11 19 110 170 F2 19 11 56 M9 39 54 33 F16 45 90 178 B12 16 229 144 F3 20 141 26 N10 43 56 34 G11 56 219 245 B13 9 105 242 F4 17 10 124 N11 50 186 70 G12 46 214 192 B14 6 224 211 F5 18 140 94 N12 53 61 97 G13 43 89 175 B15 1 101 240 G1 25 14 122 N13 63 66 27 G14 44 213 189 B16 2 100 210 G2 26 144 92 N5 14 168 77 G15 41 88 176 B3 61 131 225 G3 23 13 53 N6 17 43 108 G16 42 212 190 B4 58 250 198 G4 24 143 23 N7 24 173 10 H10 33 84 150 B5 51 126 162 G5 21 12 121 N8 27 48 38 H11 36 209 185 B6 48 245 134 G6 11 7 156 N9 40 181 6 H12 39 87 153 B7 41 121 232 G7 7 5 155 010 44 183 3 H13 40 211 187 B8 38 240 201 H1 32 147 21 011 47 58 31 H14 37 86 152 B9 29 115 233 H2 29 16 119 012 54 188 67 H15 38 210 188 C10 23 112 167 H3 30 146 89 013 57 63 98 H16 35 85 154 C11 20 231 139 H4 27 15 54 03 3 36 44 H9 60 221 215 C12 13 107 237 H5 28 145 24 04 10 166 80 110 34 208 186 C13 10 226 212 H6 31 17 51 05 13 41 107 111 31 83 151 C14 3 102 172 H7 34 148 90 06 20 171 9 112 28 205 59 C4 57 129 228 11 35 19 52 07 23 46 37 113 27 81 149 C5 54 248 197 12 38 150 87 08 30 176 73 114 30 206 62 C6 47 124 161 13 37 20 117 09 37 53 101 115 29 82 148 c7 44 243 133 14 40 151 19 P1 2 34 82 116 32 207 61 C8 37 119 231 15 39 21 49 P10 41 55 102 K10 7 71 63 C9 30 236 203 16 36 149
60. 9600000 H ee se BLAD d b ob ib ib 000606000 e009000009000000000 Example Well A The numbering of MEA electrodes in the 7 x 6 grid per each well follows the standard numbering scheme for square grids A32 A42 A52 The first digit is the column number and the second CA WX digit is the row number For example electrode 23 PA is positioned in the second column of the third row A34 A44 A54 There is one big internal reference electrode around each well The six reference electrodes are connected to two of the four contact pads for grounding them Multi Channel Systems Fon 49 7121 9 09 25 O 2014 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 256 6wellMEA multichannel systems Stim Elect Sprin Hard Stim Elect Spri Hard Stim Elec Spri Hard Stim Elect Spri ard Sock rod g ware Sock rode ng war Sock trod ng war Sock rode ng v are et ID Con ID et ID Con eID et eID Con eID et ID Con D 1 A46 2 226 1 B72 35 112 1 D41 68 96 1
61. A64 ASS A45 B34 Boa ES G13 Bid G2 831 G42 d52 B43 GS B64 855 B45 E42 E52 GND AG5 A25 A14 AS A 3 A22 AG2 A41 AS A62 AGO A53 A54 A44 B35 B25 B14 833 623 822 832 B41BS1 B62 BES B59 B54 B44 E41 E51 EG2 E49 39 C35 Ese E22 E21 C25 C34 3 Ete C14 C24 045 E13 cas csi D55 D44 C33 Cis D64 D54 Ce Ge D43 Des cat C31 D52 D62 C51 Ca oos cron D31D41 63 053 D21D32 643 6S D12022 ost cos p19 52s ee D33 DS D C45 624614 F51 F4 nans rer ee Fs2 Fas 9G F53 Fea Gi G22 F54 Fea GS G3s 44 F55 Gis F35 F45 G24G14 F25 F34 G34G25 Fia faa BS ee GS Gas oe 645s F22 F12 G63 Gas F32 F21 ee E83 F31 Se E64 Ess 642 651 E55 E54 ES1G41 E44GND H14 H25 H35 H44 H54 HSS H43 62 H51 E69 E14 BS E35 Hao He A Hio ita Hab Hob ta dis deo dio dea OLLADE 5e 162 d GND E24 E24 H31 H21 HI2 HS1 H39 H24 Had H45 H55 Hed Hes HS2 HS H42 Jat Ji JI2JS1 J33 J24 J34 5 JS i64 63 Jas 52 42 E45 fz bas A Example Well A The numbering of MEA electrodes in the 6 x 5 grid per each well follows the standard numbering scheme for square grids fang 22 fas The first digit is the column number and the second digit is the row number For example electrode 23 fas is positioned in the second column of the third row Two square electrodes 1 and 2 per well are available for stimulation or recording There is one big internal reference electrode in each has bas well The nine reference electrodes are connected to four contact pads for grounding them Multi Channel Sy
62. Au The insulation material is silicon nitride This type of MEA is characterized by very low impedance values of approximately 20 kO They are ideal for stimulation and have excellent biocompatibility and cell adhesion Important MEAs are not symmetrical That is why the writing for example NMI LEITER MEA type on the MEA chip should be on the right side viewed from the front with the sockets of the amplifier in the back MEAs with one big internal reference electrode should be placed with reference electrode to the left side in the amplifier Otherwise the MEA layout will not match with the pin layout of the channel map in the data acquisition program Using 60PedotMEAs MEAs with PEDOT CNT electrodes can be used for a wide variety of applications They are robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Generally they can be used for acute experiments as well as long term cultures 29 MEA Manual 4 18 FlexMEA FlexMEAs are made of flexible polyimide foil perfect for in vivo and specific in vitro applications Only 12 um thick and weighing less than 1 g the FlexMEA biosensor is very thin and light weight The FlexMEAs are available with 32 64 recording electrodes plus two four indifferent reference electrodes and two four ground electrodes in a 6 x 6 8 x 9 electrodes grid More layouts can be provided on request The flexible base is perforated for
63. CS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice CH25 CH26 systems CH31 CH32 CH24 CH27 CH28 CH30 STG24 STG26 STG13 STG12 STG11 STG14 multichannel pMEA32S12 Layout 1 systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System MC Rack channel map pMEA 32812 L1 12x3 cmp 15 14 11 12 16 29 17 18 21 20 6 8 10 4 13 2 31 19 26 23 22 25 9 7 5 3 1 30 28 27 24 32 The MC Rack channel map is build analog to the layout of the recording electrodes in the grid A4 A7 A10 A13 A16 A19 A22 A25 A28 A31 A1 A3 A6 AQ A12 A15 A18 A21 A24 A27 A30 A32 A2 A5 A8 Alii Ai4 Ai7 A20 A23 A26 A29 Table Correlation of MC Rack channels and recording electrodes A Number of recording electrode CH Channel number in MC Rack S Number of stimulation electrode STG Internal stimulus generator connection REF Reference electrode S 1 STG 23 STG 16 S7 S2 STG 25 REF STG 13 S8 S38 STG 22 STG 15 S9 S4 STG 24 STG 12 S 10 S5 STG 21 STG 14 S 11 S6 STG 26 STG 11 S 12 A 1 CH 6 CH 30 A17 A 2 CH 9 CH 31 A18 A3 CH 8 CH 29 A 19 A 4 CH 15 CH 28 A20 A5 CH 7 CH 19 A 21 A 6 CH 10 CH 17 A22 A 7 CH 14 CH 27 A 23 A 8 CH 5 CH 26 A 24 A 9 CH 4 CH 18 A25 A10 CH 11 CH 24 A 26 A 11 CH 3 CH 23 A27 A 12 CH 13 CH 21 A 28 A13 CH 12 CH 32 A 29 A14 CH 1 CH 22 A 30 A15 CH 2 CH 20 A 31 A16 CH 16 CH 25 A 32 Multi Channel Systems Fon 49 7121 9 09 25 O
64. E15 101 240 2 F74 1 196 2 B62 34 82 2 C13 67 66 2 E25 100 210 3 A36 3 158 3 B73 36 44 3 D51 69 28 3 E14 102 172 4 A44 133 130 4 B63 163 13 4 p43 193 126 4 E24 223 141 5 A26 4 223 5 B74 37 109 5 D61 70 64 5 E13 103 241 6 A35 134 193 6 B54 164 79 6 p52 194 214 6 E33 224 211 7 A25 5 155 7 B75 38 41 7 pe2 71 63 7 12 104 173 8 A16 135 253 8 B64 165 14 8 D71 195 217 8 E23 225 122 9 A24 6 224 9 B76 39 110 9 p63 72 244 9 E11 105 242 10 A15 136 194 10 B65 166 80 10 D72 196 216 10 E22 226 212 11 A34 7 156 11 B66 40 42 11 D53 73 247 11 E21 106 174 12 A14 137 254 12 B44 167 11 12 D73 197 219 12 43 227 143 13 A23 8 123 13 B56 41 107 13 D64 74 246 13 E31 107 237 14 A13 138 93 14 B55 168 77 14 D74 198 218 14 E32 228 207 15 A22 9 55 15 B46 42 39 15 D65 75 249 15 E41 108 169 16 A12 139 25 16 B45 169 12 16 D75 199 222 16 42 229 144 17 A33 10 124 17 B36 43 108 17 D54 76 248 17 E51 109 238 18 A11 140 94 18 B35 170 78 18 D76 200 220 18 E52 230 208 19 A32 11 56 19 B26 44 40 19 D55 77 222 19 E61 110 170 20 A21 141 26 20 B25 171 9 20 D66 201 221 20 E62 231 139 21 A42 12 121 21 B16 45 105 21 D45 78 250 21 E71 111 235 22 A31
65. MCS 1 2mm 0 8 mm 0 8 mm 1 2 mm OOOO Oooo Slot to insert mjnemamauamanamasaassaRSSRRRSEC an O ring for T ru e 05 membrane Eu DH 3 B B covering Ln H EH oH EH o rd Urn EH Lr o pd EH mm gH Lr 3 En oH En o ELI OF HO oO d OF B l b OF oH TY Y F o E 3 goH da rH B o5 pH um f oF Or A m o X oH A 1 Ba pH Dj M E ED minimini minimin im mim 9 Well macrolon 256 9wellMEA with macrolon quadrat for quadrat and 9well CC ring 256 9wellMEA to use it as a culture chamber Please insert a foil between quadrat and ring Please insert the 256 9wellMEA in correct orientation into the amplifier If you can not read the MCS use the black reference electrodes as marker Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems E31 AB A24 ABB As Ale AGI AGI A42 A52 A49 AS2
66. Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 256MEA multichannel systems Stimulation Connector Socket 1 Stimulation Connector Socket 2 Stimulation Connector Socket 3 Stimulation Connector Socket 4 Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID A2 2 1 196 19 60 191 125 C15 61 98 239 A10 25 113 236 B1 4 133 130 K7 7 38 41 C16 62 222 209 A11 22 232 205 B2 1 2 226 K8 34 178 74 D13 63 99 171 A12 15 108 169 C1 5 4 223 K9 33 51 104 D14 57 96 184 A13 12 227 143 C2 6 134 193 L10 56 189 2 D15 58 220 213 A14 5 103 241 C3 3 3 158 L11 59 64 30 D16 55 95 181 A15 4 223 141 D1 12 137 254 L7 11 40 42 E13 53 94 182 A3 62 252 195 D2 9 6 224 L8 31 50 35 E14 54 218 243 A4 55 128 159 D3 10 136 194 L9 36 179 5 E15 51 93 179 A5 52 247 131 E1 15 9 55 M10 46 184 69 E16 52 217 147 A6 45 123 229 E2 16 139 25 M11 49 59 100 F11 59 97 183 A7 42 2
67. No 3 B34 Conn 1 No 45 C21 Conn 2 No 25 of stimulation Aas Conn 1 No 4 B14 Conn 1 No 46 c12 Conn 2 No 26 A26 Conn 1 No 5 B23 Conn 1 No 47 c31 Conn 2 No 27 connector socket A35 Conn 1 No 6 B13 Conn 1 No 48 C22 Conn 2 No 28 A25 Conn 1 No 7 B22 Conn 1 No 49 c41 Conn 2 No 29 in well A B and C A16 Conn 1 No 8 B12 Conn 1 No 50 c32 Conn 2 No 30 A24 Conn 1 No 9 B33 Conn 1 No 51 C51 Conn 2 No 31 A15 Conn 1 No 10 B11 Conn 1 No 52 c42 Conn 2 No 32 A34 Conn 1 No 11 B32 Conn 1 No 53 c61 Conn 2 No 33 A14 Conn 1 No 12 B21 Conn 1 No 54 c52 Conn 2 No 34 A23 Conn 1 No 13 B42 Conn 1 No 55 c71 Conn 2 No 35 A13 Conn 1 No 14 B31 Conn 1 No 56 c43 Conn 2 No 36 A22 Conn 1 No 15 B43 Conn 1 No 57 C72 Conn 2 No 37 A12 Conn 1 No 16 B41 Conn 1 No 58 C62 Conn 2 No 38 A33 Conn 1 No 17 B52 Conn 1 No 59 c73 Conn 2 No 39 A11 Conn 1 No 18 B51 Conn 1 No 60 c63 Conn 2 No 40 A32 Conn 1 No 19 B53 Conn 1 No 61 C74 Conn 2 No 41 A21 Conn 1 No 20 B61 Conn 1 No 62 C53 Conn 2 No 42 A42 Conn 1 No 21 B63 Conn 1 No 63 75 Conn 2 No 43 A31 Conn 1 No 22 B72 Conn 2 No 1 C64 Conn 2 No 44 A43 Conn 1 No 23 B62 Conn 2 No 2 C76 Conn 2 No 45 A41 Conn 1 No 24 B73 Conn 2 No 3 c65 Conn 2 No 46 A52 Conn 1 No 25 B63 Conn 2 No 4 C66 Conn 2 No 47 A51 Conn 1 No 26 B74 Conn 2 No 5 c54 Conn 2 No 48 A53 Conn 1 No 27 B54 Conn 2 No 6 C56 Conn 2 No 49 A61 Conn 1 No 28
68. O O 9 mea pins N N eo N o N eo N N o 8 ul vi ul N N N N o N o w N w e A ul A o A eo A N ui N ul eo 56 55 68 67 78 Electrode 55 es 6 7s MEA pins MEA pins Electrode w o o The numbering of MEA electrodes in the 8 x 8 grid follows the standard numbering scheme for square grids The first digit is the column number and the second digit is the row number For example electrode 23 is positioned in the third row of the second column The specified MEA pin numbers are the channel numbers that are used in the MC Rack program when using the 2 dimensional layout in Data Source Setup The electrode 15 is replaced by a big internal reference electrode Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60PedotMEA200 30iR Au multichannel systems Microelectrode Array with PEDOT CNT Electrodes amp 60 606060 G GO 6 6 99 Q 6 6 6 6 0020G Q 6 6 6 60020 amp 6 6000000 e amp GO Technical Specifications GOPedotMEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre
69. Pin 5 B3 3 B2 B3 Pin 6 E3 4 27 Pin 7 C3 5 Pin 8 F3 6 c2 c3 Pin 9 not connected 7 25 Pin 10 G3 8 Pin 11 not connected 9 D eS Pin 12 H2 10 32 Pin 13 not connected 11 E2 E3 Pin 14 H3 12 Pin 15 not connected 13 30 Pin 16 J3 14 F2 F3 Pin 17 not connected 15 8 26 5 Pin 18 K3 16 Pin 19 not connected 17 G1 G2 G3 Pin 20 K2 18 Ga Pin 21 not connected 19 Pin 22 K1 20 H1 H2 H3 Pin 23 not connected 21 Pin 24 n 22 22 Pin 25 not connected 23 J1 J3 Pin 26 H1 24 Pn 275 2 25 60 19 Gs Pin 28 G2 26 K1 K2 K3 Pin 29 C2 27 Pin 30 G1 28 Pin 31 B2 29 Warning The device may only be used together with Pin 32 F2 30 the MPA32I from Multi Channel Systems MCS GmbH as 2 e 2 and only for the specified purpose Damage of the in pi Pin 35 Ref Stim Bottom device and even injuries can result from improper use Pin 36 GND Top Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel EcoFlexMEA36 systems Flexible Microelectrode Array with 36 electrodes for use with 32 Channel Miniature Preamplifier MPA32I for in vivo or in vitro applications Electrode field 1800 x 1800 um 32 recording electrodes 2 reference electrodes
70. S GmbH Product information is subject to change without notice N N o Fr n CH22 CH25 CH26 CH31 CH32 CH24 CH27 CH28 CH30 STG24 STG26 STG13 STG12 STG11 STG14 e multichannel pMEA32S12 Layout 2 systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System MC Rack channel map pMEA 32812 L2 12x3 cmp 5 2 8 14 13 19 21 22 31 27 1 3 9 6 15 12 17 20 25 32 28 30 7 4 10 11 16 29 18 23 26 24 The MC_Rack channel map is build analog to the layout of the recording electrodes in the grid A4 A7 A10 A13 A16 A19 A22 A25 A28 A31 A1 A3 A6 AQ A12 A15 A18 A21 A24 A27 A30 A32 A2 A5 A8 Ali Ai4 Ai7 A20 A23 A26 A29 Table Correlation of MC Rack channels and recording electrodes A Number of recording electrode CH Channel number in MC Rack S Number of stimulation electrode STG Internal stimulus generator connection REF Reference electrode S 1 STG 23 STG 16 S7 S2 STG 25 STG 13 S8 S3 STG 22 STG 15 S9 S4 STG 24 STG 12 S 10 S5 STG 21 STG 14 S 11 S6 STG 26 STG 11 S 12 A 9 CH 6 CH 30 A32 A6 CH 9 CH 31 A 28 A 10 CH 8 CH 29 A17 A 12 CH 15 CH 28 A 30 A2 CH 7 CH 19 A 19 A8 CH 10 CH 17 A 18 A 13 CH 14 CH 27 A31 A4 CH 5 CH 26 A 26 A5 CH 4 CH 18 A 20 A 11 CH 11 CH 24 A 29 A3 CH 3 CH 23 A23 A 16 CH 13 CH 21 A 22 A 15 CH 12 CH 32 A 27 A 1 CH 1 CH 22 A 25 A 7 CH 2 CH 20 A 21 A14 CH 16 CH 25 A 24 Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH
71. S GmbH always apply The operator will receive these no later than on conclusion of the contract Multi Channel Systems MCS GmbH makes no Guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the validity of his findings Guarantee and liability claims in the event of injury or material damage are excluded when they are the result of one of the following mproper use of the device Improper installation commissioning operation or maintenance of the device Operating the device when the safety and protective devices are defective and or inoperable Non observance of the instructions in the manual with regard to transport storage installation commissioning operation or maintenance of the device Unauthorized structural alterations to the device Unauthorized modifications to the system settings Inadequate monitoring of device components subject to wear Improperly executed and unauthorized repairs Unauthorized opening of the device or its components Catastrophic events due to the effect of foreign bodies or acts of God MEA Manual 2 3 Important Safety Advice A Warning Make sure to read the following advice prior to install or to use the device and the software If you do not fulfill all requirements stated below this may lead to malfunctions or breakage of connected hardware
72. See also Potter S M Wagenaar D A and DeMarse T B 2005 Closing the Loop Stimulation Feedback Systems for Embodied MEA Cultures Advances in Network Electrophysiology Using Multi Electrode Arrays M Taketani and M Baudry Springer Wagenaar D A Madhavan R Pine J and Potter S M 2005 Controlling bursting in cortical cultures with closed loop multi electrode stimulation J Neurosci 25 3 680 8 For pacing cardiomyocytes higher voltages and durations are generally required for example 2 V for 2 ms As these pulses are not supported by standard MEA electrodes the use of larger stimulating electrodes is recommended A special MEA with four pairs of large 250 x 50 uim stimulating electrodes 60StimMEA200 30 and a special stimulation adapter is provided for such applications by Multi Channel Systems pulses applying the negative phase first Always regard the safe charge injection limits as described in Warning When using MEA electrodes of TiN material use only negative voltages pulses or biphasic current the appendix of this manual Otherwise electrodes can be irreversibly damaged by electrolysis 56 Troubleshooting 9 Troubleshooting 9 1 About Troubleshooting The following hints are provided to solve special problems that have been reported by users Most problems occur seldom and only under specific circumstances Please check the mentioned possible causes carefully when you have any trouble with
73. TIM4 pMEA 32812 L2 12x3 cmp Rinse with distilled water Do not use ultrasonic bath A Do not autoclave or sterilize pMEAs by heat These MEA types are not heat stable and will be rreversibly damaged Cleaning pMEA perfusion chamber w o Without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm Multi Channel Systems Fon 49 7121 9 09 25 O MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Germany www multichannelsystems com 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice PMEA32S12 Layout 2 multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System PES E E EGE Oo OOO Yj S L Direction to Amplifier The oval area of the pMEA chip is perforated Electrode layout in the grid 90 um 6 100 um 50 um A 1 90 um A4 AY 150 um A3 A6 amp A5 30 um Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany A10 A 9 CH19 CH29 CH16 CH13 CH12 CH11 CH14 CH15 CH10 CH 8 CH 6 CH 4 A13 A16 A19 A12 A15 A18 A11 A14 A17 NS NN IN oe A22 A25 A28 A31 A21 A24 A27 A30 A recording electrode stimulation electrode Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com 2013 Multi Channel Systems MC
74. Ti 60HexaMEA ITO provides no internal reference electrode The electrodes of 6 0HexaMEA40 10iR ITO are configured with invariable interelectrode distance of 40 um and with TiN electrodes of 10 uim diameter They include a big internal reference electrode The tracks and contact pads are made of ITO Using 60HexaMEAs The same material is used for standard MEAs and HexaMEAs Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures 16 MEA Manual 4 5 Thin MEA 60ThinMEA 60ThiINMEA200 30iR ITO 60ThinMEA30 10IR ITO 60ThinMEAs are only 180 um thick ideally suited for high resolution imaging 60ThinMEAs are like standard MEAs but the electrodes are embedded in a very thin and delicate glass substrate on a robust ceramic carrier The thin glass allows the use of oil immersion objectives with a high numerical aperture Like standard MEAs 59 electrodes and one reference electrode are arranged in an 8 x 8 layout grid with electrode diameters of 30 um and interelectrode distances of 200 um 60ThinMEASs are also available in a double 5 x 6 layout grid with 10 um TIN electrodes and 30 um interelectrode distance like the High Dense MEA 60ThinMEA30 10iR ITO The flat round electrodes are made of titanium nitride TiN Tracks and contact pads are made of transparent ITO insulation material is silicon nitride Using 60ThinMEAs 60ThinMEAs ar
75. a standard dish washing detergent When cleaning coated MEAs parts of the coating may go off You have to recoat a MEA when the coating is not sufficient anymore that is when you observe problems with cell attachment or recording If more severe methods are needed the MEA can also be cleaned in an ultrasonic bath for a short moment But this method is a bit dangerous because there are ultrasonic baths that are too strong and will destroy the MEA The behavior should be tested with an older MEA first Generally is using an ultrasonic bath not recommended EcoMEAs are easier to clean because the golden electrodes are not so easily damaged Cleaning of pMEAs Perforated MEAs have a robust ceramic or glass carrier but the electrodes are embedded in polyimide foil Therefore they are heat stable to 50 C only and cannot be autoclaved Please do not use ultrasonic bath for pMEAs Rinse with distilled water first then apply 196 Terg A Zyme solution Sigma for several hours Rinse the pMEA again with distilled water and dry them directly before use Sterilization via rinsing with 70 96 ethanol is possible Do not immerse the pMEA into the alcohol for a longer time otherwise the ring may probably get off Cleaning of ECoMEAs The gold electrodes of ECOoMEAs are very robust and are the only MEA electrodes that will endure more severe cleaning methods You can check the need for cleaning under a stereo microscope The electrodes should be shin
76. ange Germany www multichannelsystems com without notice multichannel systems EcoFlexMEA36 Electrode layout em 0066 cmm 696 666666 606060 00000A e00000 6 wn 90 GND 1 is a large ground electrode connected to pin 1 of the MPA32I input connector GND 2 is a second ground electrode connected to pin 36 The REF electrodes are reference electrodes connected to pin 2 and 35 respectively Both ground inputs and both reference electrode inputs are equal that is they are connected to each other inside the standard MPA32l Please see the MPA321 manual for details The numbers in the electrodes are the recording channel numbers that refer to the channel numbers in the MC Rack program Please make sure that you have selected Configuration in the Channel Layout under Data Source Setup with a total number of 64 channels Deselect the check box Digital input channel if you do not need it otherwise it could be that one recording channel is missing In Amplifier please choose FA32I S or FA64I S and in MEA EcoFlexMEA36 In Layout tab of the display please click Default Map The letter digit code below is the electrode identifier and refers to the position of the electrode in the grid Important The MC Rack channel map is constructed by looking on the back side of the electrodes because the FlexMEA electrodes are placed on the preparation upside down If you use a MEA64 System with more than one MPA32l t
77. annel Systems MCS GmbH Product information is subject to change without notice multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System PMEA32S 12 Layout 3 MC Rack channel map pMEA 32812 L3 12x3 cmp 19 17 18 21 23 22 26 31 32 24 13 16 29 12 20 11 4 25 7 27 28 30 14 15 10 8 6 2 9 5 3 1 The MC_Rack channel map is build analog to the layout of the recording electrode in the grid A4 A7 A10 A13 A16 A19 A22 A25 A28 A31 A1 A3 A6 AQ A12 A15 A18 A21 A24 A27 A30 A32 A2 A5 A8 Aii Ai4 Ai7 A20 A23 A26 A29 Table Correlation of MC Rack channels and recording electrodes A Number of recording electrode CH Channel number in MC Rack S Number of stimulation electrode STG Internal stimulus generator connection REF Reference electrode S 1 STG 23 STG 16 S 7 S2 STG 25 STG 13 S8 S3 STG 22 STG 15 S9 S4 STG 24 STG 12 S 10 S5 STG 21 STG 14 S 11 S6 STG 26 STG 11 S 12 A14 CH 6 CH 30 A32 A 20 CH 9 CH 31 A 25 A 11 CH 8 CH 29 A6 A5 CH 15 CH 28 A 30 A 24 CH 7 CH 19 A4 A8 CH 10 CH 17 A7 A2 CH 14 CH 27 A 27 A 23 CH 5 CH 26 A 22 A 18 CH 4 CH 18 A 10 A 15 CH 11 CH 24 A31 A26 CH 3 CH 23 A 16 A 1 CH 13 CH 21 A 13 A 9 CH 12 CH 32 A 28 A 29 CH 1 CH 22 A 19 A17 CH 2 CH 20 A 12 A3 CH 16 CH 25 A21 Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is
78. anol You can adjust the concentration to meet your requirements Procedure Directly before use pipette 3 5 ul of the working solution onto the recording field The recording field should be completely covered Remove the coating solution and let the MEA air dry It takes just a few seconds for the methanol to evaporate Literature Ulrich Egert Thomas Meyer 2004 Heart on a Chip Extracellular multielectrode recordings from cardiac myocytes in vitro Methods in Cardiovascular Research S Dhein and M Delmar eds Coating with Polyethyleneimine PEI plus Laminin Polyethyleneimine PEI has been successfully employed for dissociated cell cultures and proven to enhance cell maturation in culture compared to polylysine coated plates Polyethyleneimine is a positively charged polymer and thus changes the charge on the glass surface from negative to positive The tissue sticks even better with this method than with the nitrocellulose method but the polyethylenimine forms a uniform layer that can get more easily detached from the surface for example by the perfusion This coating method can optionally be combined with laminin Materials Poly ethyleneimine solution PEI Sigma Aldrich Inc P3143 Boric acid crystalline Fisher Scientific A73 500 Borax sodium tetraborate Sigma Aldrich Inc B0127 1NHCI Laminin 1mg ml Sigma Aldrich Inc L2020 Borate buffer 3 10 g boric acid 4 75 g borax Dissolve i
79. arate culture chambers on one MEA for example for drug application in a screening experiment 9 well MEAs for use with USB MEA256 System 256 electrodes in nine blocks of 26 recording two stimulation and reference electrodes each Using the 9 well MEA with macrolon quadrant you have 9 separate culture chambers for example for drug application in a screening experiment 120MEAs and a perforated 120pMEA with 12 x 12 layout grid for use with MEA2100 120 System only 12 MEA Manual e Square MEAs with TIN Titanium nitride electrodes in 50 x 50 um square size in a 8 x 8 layout grid e PEDOT CNT MEAs with carbon nanotube poly 3 4 ethylene dioxythiophene electrodes and gold tracks and contact pads have very low impedance values of approximately 20 kQ They are ideal for stimulation and have excellent biocompatibility and cell adhesion e FexMEAs made of flexible polyimide 2611 foil perfect for in vivo and specific in vitro applications for example whole heart preparations Available with 36 FlexMEA36 or 72 FlexMEA72 TiN Titanium nitride electrodes e EcoFlexMEAs made of flexible polyimide Kapton as well but very cost efficient and more robust than FlexMEAs from polyimide foil Available with 36 EcoFlexMEA36 or 24 EcoFlex24 gold electrodes 4 1 Standard Electrode Numbering 000000 6 amp OOOO O 6 amp 9 OOOO 9 69 G9 69 G9 69 9 6 6 6 6 6 6 6 G9 G9 G9 69 C9 69 amp amp
80. arts in this manual that refer to the applications and not to the product itself for example coating of MEAs are only a summary of published information from other sources see references and has the intention of helping users finding the appropriate information for setting up their experiments Multi Channel Systems MCS GmbH has not tested or verified this information Multi Channel Systems MCS GmbH does not guarantee that the information is correct Multi Channel Systems MCS GmbH recommends to refer to the referenced literature for planning and executing any experiments Microelectrode Arrays MEAs Overview 2 Important Information and Instructions 2 1 Operator s Obligations The operator is obliged to allow only persons to work on the device who gre familiar with the safety at work and accident prevention regulations and have been instructed how to use the device gre professionally qualified or have specialist knowledge and training and have received instruction in the use of the device have read and understood the chapter on safety and the warning instructions in this manual and confirmed this with their signature It must be monitored at regular intervals that the operating personnel are working safely Personnel still undergoing training may only work on the device under the supervision of an experienced person 2 2 Guarantee and Liability The General conditions of sale and delivery of Multi Channel Systems MC
81. ase material Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems Aspenhaustrasse 21 72770 Reutlingen w o Without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm pr Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm pr T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O MCS GmbH Fax 49 7121 9 09 25 11 info multichannelsystems com Germany www multichannelsystems com multichannel systems 0 125 C 49 mm x 49 mm x 1 mm Glass Ti Titanium TiN Titanium nitride 30 um 200 um inside of the quadrants 1000 um between the quadrants 500 um from quadrants to the center line Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD Approximately 30 50 kQ 4x 1x4 1x5 1x4 center line 1 x 7 59 1 internal reference electrode iR 2 dim MEA 4QMEA cmp 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice multichannel 60 4QMEA1000 systems 60 4QMEA1000iR Ti Electrode layout 5 00000000000000000 Electrode B1 o s 2 C1 33 21 44 54 71 63 C7 82
82. ater Do not use ultrasonic bath Do not autoclave or sterilize pMEAs by heat These MEA types are not heat stable and will be rreversibly damaged w o Without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System pMEA32S12 Layout 4 REF 4 N O r 7 STG15 N CH31 CH29 4 CH32 a A Fi CH15 ZZ CH22 STG14 STG11 STG26 CH 2 o Q Hi F CH14 w ZRN WA CH16 S CH11 MeL 31 E T CH20 S CH12 ma CH26 5 CH13 9 E ITI 7 fy Se CH21 AA INN 6430 a T AANA 2 PISA CH25 7 ANS s eod dm Bem n 2 aN RE M S TES I o um ur xo 000 Oo oO Oo The oval area of the pMEA chip is perforated OG DOO DO 4 gi D Electrode layout in the grid 100 um 2209 A20 A25 A21 A17 A13 A9 A5 A1 100 um A30 A26 A22 A18 A14 A10 A6 A2 A recording electrode w 29 amp 9 amp 5 Gm o E stimulation electrode amp A28 A24 A20 A16 A12 A8 A4 30 um 0 um Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is s
83. channel systems e Connector 1 Connector 4 gt Z 10129uu023 1 e v ABCDEFGHIKLMNOPR v Technical Specifications 256MEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Contact pads for reference electrodes connected to ground MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany w o X Without ring Connector 3 d 0 125 C 49 mm x 49 mm x 1 mm Glass Indium tin oxide ITO 8 10 or 30 um 30 60 100 or 200 um Planar Titanium nitride TiN Silicon nitride SiN 30 50 kQ for 30 um or 250 400 kQ for 10 um 16x 16 252 4 internal reference electrodes iR 4 Configuration 16 x 16 cmp gr Glass ring ID 19 mm OD 24 mm height 6 12 mm pr Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm pr T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com 2013 Multi Channel Systems MCS GmbH Product information i
84. chnical Specifications G0pMEA100 30IR Ti Temperature compatibility Dimension W x D x H Base material Perforation Diameter of innermost area Total area of holes Diameter of holes Contact pads Track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of electrodes Reference electrodes MC Rack Source layout in Data Source Setup Channel map Cleaning MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany A w o gr pr pr T Fon 49 7121 9 09 25 O Rinse with distilled water These pMEAs are not heat stable and should not be autoclaved 10 50 C 49 mm x 49 mm x 1 mm Polyimide foil 2611 on glass or ceramic carrier 2mm 19 according to 2 mm 5 6 7 5 9 10 12 5 15 17 7 20 TiAu Titan Gold TiAuTi Titan Gold Titan 30 um 100 um Planar TiN Titanium nitride Polyimide foil 2610 isolator 30 50 kQ 6x10 60 with internal reference iR 2 dim MEA pMEA 6x10 cmp Do not use ultrasonic bath Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm 2013 Multi Channel Systems MCS GmbH Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsys
85. ck the problem e The serial number of the MEA You will find it on the MEA case e The amplifier type and serial number You will find it on the device e The operating system and service pack number on the connected computer e The hardware configuration microprocessor frequency main memory hard disk of the connected computer This information is especially important if you have modified the computer or installed new hard or software recently e The version of the recording software On the Help menu click About to display the software version 57 9 3 MEA Manual Noise on Single Electrodes The noise level on single electrodes is significantly higher than expected or you see artifact signals In the following example 60MEA200 30 filled with PBS phosphate buffered saline silver pellet as bath electrode shielded electrodes No 53 63 73 45 55 48 58 show a high noise level E Standard Noise with Shielding 525 000sec Xs 1000 Ims oo soo 525th sweep Ys 20 iV gt 200 200 Mop 200 00 goo sop goo so p go P g P 200 op goo Mop Qoo doo 200 so goo 200 j S E P el rr ie el PRP ye Possible causes The electrode or the contact pin of the amplifier may be defective To test this do the following Open the amplifier and turn the MEA by 90 degrees Close the amplifier again and start the recor
86. com Germany www multichannelsystems com 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice multichannel systems 120MEA 120MEA200 30iR Ti 120MEA100 30iR ITO E5 D2 J1 J3 K2 C2 D3 D1 E3 E1 H4 J2 J4 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 C3 F4 F2 F5 G6 G1 F3 F1 G5 G2 B3 45 GND GND 76 K3 D4 44 77 13 c4 43 78 5 DES ne 8608668 mee ee os 060606060000 E s e o 66 6 6002060 m ce WS Ad B4 Ch 04 E4 FA G4 HA J4 Ka L4 Mi m se SB6SOGEBGCBECEG m E6 M6 ro Tat e ee066066000000 735 30 91 G7 B 29 A7 B7 c7 D7 amp e G7 H7 K7 b M7 92 H7 28 93 m7 e 27 AB B8 ce D8 E8 F8 G8 H8 J8 Ka 18 M8 94 L7 p A9 B9 C9 D9 E9 F9 G9 H9 JI K9 L9 MI s Bs 24 97 we co 23 00909020000 98 Le D8 22 nv k 99 ks as 21 0e0600000970 100 ve B9 20 101 m9 co 19 REF Di2 E12 F12 Gi2 H12 912 REF 102 L9 E8 18 103 ke B1017 104 79 C10 16 OND GND jos L10 1514131211109 87 6 5 43 2 1 120119118 117 116 115 114 113 112 111 110 109 108 107 106 D9 E1 F9 F11 F8 G12 G10 H12 H10 J12 J10 K11 11 11 D11 E9 1 C11 D10 D12 E10 E12 F10 F12 F7 G8 G11 G9 H9 H8 K10 The letter digit code is the electrode identifier and refers to the position of the electrode in the 12 x 12 layout grid The number code is the hardware identifier of the electrode The reference electrodes REF are connected to ground GND Please insert the 120MEA in correct o
87. cs NB Ad B4 C4 D4 G4 F4 Ge H4 J4 Ke L4 M m s I e e o 6 6 6 6 O6 6 6 6 6 m E6 M6 re 31 0e6060660606006000000 375 30 91 G7 p 29 A7 B7 c7 D7 amp amp G7 H7 KT G M7 92 H7 28 93 M7 p 27 AB B8 ce D8 E8 F8 G8 H8 J8 K8 L8 M8 94 17 2 A9 B9 C9 DI E F G9 H9 J9 k 19 m9 op n Bs 24 97 m8 T 60909000000 m D8 22 99 ks ao 21 000000207 100 ve B9 20 101 we co 19 REF Di2 E12 F12 Gi2 H12 J12 REF 102 L9 E8 18 103 ko B1017 104 v9 C10 16 OND 105 L10 1514131211109 8 7 6 5 43 2 1 120119 118 117 116 115 114 113 112 111 110 109 108 107 106 D9 D11 E9 E11 F9 F11 F8 G12 G10 H12 H10 J12 J10 K1 C11 D10 D12 E10 E12 F10 F12 F7 G8 G11 G9 H11 H9 11 H8 K10 The letter digit code is the electrode identifier and refers to the position of the electrode in the 12 x 12 layout grid The number code is the hardware identifier of the electrode The reference electrodes REF are connected to ground GND Please insert the 120pMEA in correct orientation when looking from the front into the opened headstage The small arrow near to the contact pads of the 120pMEA faces upwards Under microscope control you can read the markers A1 and A12 M1 and M12 as shown on the diagram Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice m
88. culture first then switch to 60EcoMEA or 60EcoMEA Glass 60EcoMEAs are provided in the typical 8 x 8 layout with internal reference electrode Custom layouts following your personal specifications are possible at very reasonable prices Please ask your local retailer for details Electrodes tracks and contact pads are made of pure gold Due to the soft gold material of the contact pads the contact to the amplifier pins is excellent The insulation material on ECOMEA Glass chips is SU 8 a photoresist 1 2 um Using 60EcoMEAs Like standard MEAs 60EcoMEAs are very robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures The gold electrodes are very robust too and are the only MEA electrodes that will endure more severe cleaning methods New 60EcoMEAs are very hydrophobic They should be coated with nitrocellulose or treated with a plasma cleaner before use 18 MEA Manual 4 7 Stimulation MEA 60StimMEA 60StimMEA200 30 Ti 9 1 SO amp amp 66260 99990009 9960066606 5 6199999906 GO gz 00000000 s 99999909 amp amp amp 6 amp e 969202929 OG a Stimulation MEAs are available in 8 x 8 standard MEA layout with additional 16 stimulation electrodes Eight pairs of the stimulation electrodes are big and square the other eight pairs have the same size as the recording electrodes 30 um For perfect use with
89. d a positive net charge on the electrode 55 MEA Manual 8 4 Recommended Stimulus Amplitudes and Durations The higher the amplitude and the longer the stimulus the higher is the impact on the electrode performance Therefore the amplitude and duration should be as low as possible It is advisable to start with a low amplitude and duration and then increase it slowly until responses are evoked The allowed product of amplitude and duration is directly proportional to the electrode surface The higher the amplitude the shorter is the maximum duration of the pulse and vice versa Do not apply pulses with a higher amplitude or for a longer time than is recommended for the electrode type TiN electrodes have a rough surface structure and therefore have a larger surface than electrodes of the same size but made of a different material The safe charge injection limits in the appendix describe the relationship between maximum pulse amplitude and time for TiN electrodes As a consequence of the points discussed above Multi Channel Systems recommends using negative monophasic voltage pulses to make sure that the voltage level of the stimulating electrode is zero and thus the electrode is discharged at the end of the pulse According to the experience of MEA users voltage pulses should be lt 1 V 100 mV to 900 mV for neuronal applications to avoid damage to electrode and cells Generally pulse durations between 100 to 500 us are used
90. d are perfect for long time experiments up to several weeks and even months The electrode impedance ranges between 30 kO and 50 kO Tracks are made of titanium Ti and contact pads are made of titanium nitride TiN insulation material is silicon nitride SiN Using 60 4QMEA1000 The 60 4QMEA1000 can be used for a wide variety of applications They are robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Generally they can be used for acute experiments as well as long term cultures 27 MEA Manual 4 16 Square MEA 60SquareMEA 60SquareMEA200 50IR Ti r8 8 8 8 8 8 Es 53 E El ZERE Bs Bs Bs Bs Bs Bs Es Bs Bs s Bs s Es Be Be Bs Ez Ez e Ez s ss Bs Bs 8 8 8 8 8 8 8 8 ES S 8 9 9 3 8 3 60SquareMEAs have 60 electrodes in an 8 x 8 layout grid with square electrode of 50 x 50 um size and interelectrode distances of 200 um They are available with an internal reference electrode You can connect the internal reference electrode directly to the amplifier s ground and will not need silver pellets for grounding the bath anymore The flat square electrodes are made of titanium nitride TiN The electrode size of 50 x 50 uim guarantees very low noise MEAs with TiN electrodes are very stable Therefore the MEAs can be reused several times and are perfect for long time experiments up to several
91. d in 60pMEA100 30iR Ti The electrodes are integrated into a thin polyimide foil This thin foil is fixed on a ceramic or glass waver for mechanical stability In the middle of the waver under the electrode field there is a hole that makes it possible to access the electrode field from below The area around the electrodes is perforated to allow a perfusion of the tissue from both sides The total area of the holes averages 0 8 mm the diameters of the holes varies between 20 um and 90 um These pMEAs are designed for use with MEA2100 System headstages with perfusion element PE and MEA 1060 amplifier equipped with a perfusion ground plate PGP The PE or PGP replaces the standard ground plate of the headstage or MEA1060 amplifier Please note that there are different types of the MEA PGPs for different amplifier types MEA1060 UP PGP MEA1060 UP BC PGP and MEA1060 INV INV BC PGP Additional to the use of 60pMEAs together with the MEA2100 2x 60 System you can use 120pMEAs with MEA2100 120 System equipped with a perfusion element PE integrated in the ground plate of the headstage For an overview of suggested configurations to work with 60pMEAs see the MEA Application Note Acute Hippocampal Slices on pMEAs Using 60pMEAs Perforated MEAs have a robust ceramic carrier or they are mounted on glass as usual but the electrodes are embedded in polyimide foil Therefore they are heat stable to 50 C only and cannot be autoclaved Please do
92. ding If the same electrode in the MEA layout is affected the amplifier s contact is not ok If another electrode is now affected and the previously affected electrode is ok now the MEA electrode is not ok but the amplifier is fine The following screen shot shows the same MEA than above that has been turned clockwise by 90 degrees You see that different channels are now affected which indicates that the amplifier is fine but some electrodes on the MEA are defective ii Turning MEA by 90 degrees 1278000 sec XAxis 1000 Ims gt oo H swo 1278thsweep YAxs 20 W gt 200 200 p joo 40 0 goo sop goo Mop Qoo doo uo e 200 sop goo sop goo so 5r Use the test model probe to test the amplifier If the noise level is fine without the MEA bad MEA electrodes cannot be the cause 58 Troubleshooting MEA is defective MEAs wear out after multiple uses or over a longer time of use for example for long term cultures This is considered a normal behavior MEAs are also easily damaged by mishandling for example if wrong cleaning solutions or too severe cleaning methods are used or if the recording area is touched If you observe a bad long term performance of MEAs consider a more careful handling Possible causes The contact pads are contaminated Clean the contact pads carefully with a swab or a soft tissue and pure 100 alcohol The contact pads or the electrodes are irrev
93. drophilic Surface Treatment 5 1 1 The surface of new MEAs is hydrophobic and even hydrophilic MEAs tend to become hydrophobic again during storage A hydrophobic surface prevents attachment and growth of the hydrophilic cells The first step in preparing a MEA for use is therefore to ensure that the surface is hydrophilic enough for coating and cell adhesion To test this without contaminating the surface place a small drop of water on the MEA surface outside the culture chamber If the drop does not wet the surface you likely need to perform one of the following steps in particular when using new arrays Literature Ulrich Egert Thomas Meyer 2004 Heart on a Chip Extracellular multielectrode recordings from cardiac myocytes in vitro Methods in Cardiovascular Research S Dhein and M Delmar eds Plasma Cleaning Laboratories with access to electron microscopy facilities are likely to have a sputter device or a plasma cleaning chamber for example Plasma Technology Herrenberg Germany or PDC 32G from Harrick Plasma Ithaca NY United States MEAs can be treated in these chambers with low vacuum plasma for about two minutes The MEA surface is exposed to a gas plasma discharge which will make the surface polar and thus more hydrophilic The treatment gives a very clean and sterile surface that can be coated readily with water soluble molecules Note that the effect wears off after a few days Protein Coating If protei
94. e amp OK e Perforated Microelectrode Array in 8 x 8 layout grid for use with MEA1060 amplifiers equipped with a perfusion ground plate PGP or MEA2100 headstage equipped with a perfusion element PE The perforation allows the perfusion of the tissue from both sides of the pMEA Technical Specifications GOpMEA200 30iR Ti Temperature compatibility Dimension W x D x H Base material Perforation Diameter of innermost area Total area of holes Diameter of holes Contact pads Track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map Cleaning MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 10 50 C 49 mm x 49 mm x 1 mm Polyimide foil 2611 on glass or ceramic carrier 2mm 24 according to 2 mm 10 12 12 5 17 19 22 23 3 23 5 36 44 um TiAu Titan Gold TiAuTi Titan Gold Titan 30 um 200 um Planar TiN Titanium nitride Polyimide foil 2610 isolator 30 50 kQ 8x8 59 1 internal reference electrode iR 2 dim MEA Default Rinse with distilled water Do not use ultrasonic bath These pMEAs are not heat stable and should not be autoclaved Without ring
95. e an ultrasonic bath for cleaning A Do not autoclave or sterilize FlexMEAs by heat These MEA types are not heat stable Multi Channel Systems Fon 49 7121 9 09 25 O MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Germany www multichannelsystems com 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice multichannel FlexMEA72 systems 625 mala aud 0800 amp amp e 09 o 00 e Q D o o Electrode field 960600000 9600606060 66600000 96000000 60000060 O6 60060 lt lt MPA321 Channel 33 64 MPA321 Channel 1 32 750 um Qo LiB Direction to contact pads The numbers in the electrodes are the recording channel numbers that refer to the channel numbers in the MC Rack program Please make sure that you have selected Configuration in the Channel Layout under Data Source Setup with a total number of 64 channels Deselect the check box Digital input channel if you do not need it otherwise one recording channel is missing In Amplifier please choose FA641 S and in MEA FlexMEA72 In Layout tab of the display please click Default Map The letter digit code below is the electrode identifier and refers to the position of the electrode in the grid Note The MC Rack channel map is constructed by looking on the back side of the electrodes Please make sure to connect th
96. e electrodes MC Rack Source Layout in Data Source Setup MCS Channel map MEA perfusion chamber w o Without ring 0 125 C 49 mm x 49 mm x 1 mm Glass Indium tin oxide ITO 10 um 30 um 500 um or 150 um Planar Titanium nitride TiN Silicone nitride 500 nm PEVCD Approximately 250 400 kQ 2x 5x6 59 1 internal reference electrode 2 dim MEA HighDenseMEA cmp HighDenseMEA L cmp HighDenseMEA R cmp gr Glass ring ID 19 mm OD 24 mm height 6 12 mm pr Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice 60HiahDenseMEA multichannel dl ptr systems Electrode Layout 4 000000000000000 Electrode A3L C3L A4L ASL B4L B5L C4L C5L CIR C2R BIR B2R A1R A2R C3R A3R 12 Left electrode field Right electrode field AAR 82 23 ATL A5R 73 COOCOO mer 659 13 B2L 32 MEA pins 61 62 63 82 73 BAR 83 8 6666060 6060660 Kere 9999 13 ci p o He 83 c OR 2 es O on 15 2099 944 25660 35 16 27 45 46 56 55 77 86 65 2 G 6 Hae ee ES 26 17 36 37 38 SUDO 150 pm 68 67 66 87 76 4 26
97. e heat stabilized and can be autoclaved They can also be coated with different procedures for cell and tissue cultures They should be handled with great care because of the thin and delicate recording area 17 MEA Manual 4 6 Eco MEA 60EcoMEA 60ECOMEA 60ECOMEA Glass 60EcoMEAs are available on opaque printed circuit board PCB and on transparent glass base GOECOMEA Glass They are low price variants for medium throughput applications like small screens where material costs play a bigger role than in more scientific MEA applications 60EcoMEAs are opaque and are therefore useful only for applications where you do not need a visual control under a microscope for example for established cell cultures GOECOMEA Glasses are transparent all purpose MEAs Due to the special production process on PCB electrodes of 60EcoMEAs are available only with a diameter of 100 um and an interelectrode distance of 700 um The electrodes on 60EcoMEA Glass also have a diameter of 100 um and a distance of 700 um Thus 60ECOMEA or 60EcoMEA Glass are useful for applications where a high spatial resolution is not important but which emphasize on low price consumables They have proven to be especially useful for recordings from established cardiomyocyte cultures They are not useful for establishing a new cell culture as the cell performance cannot be monitored Multi Channel Systems recommends to use standard 200 30 MEAs for establishing the cell
98. e left hand side of the FlexMEA72 contact pads electrode side on top via ADPT FM 72 adapter to the first MPA32I channels 33 to 64 and the right hand side to the second MPA321l channels 1 to 32 Read the SC2x32 data sheet when using a signal collector The electrodes are on the same side as the contact pads The side with the writing NMI is the side with the contact pads and electrodes It might be a bit confusing that the pads look stronger from the wrong side but if you hold the FlexMEA72 into the light you see that the contact pads have a three dimensional appearance only from the correct side Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel systems EcoFlexMEA24 Flexible Microelectrode Array with 24 electrodes for use with 32 Channel Miniature Preamplifer MPA32I for in vivo or in vitro applications Top 1 35 Pin Top GND 35 33 3129 27 25 23 21 19 17 15 1311 9 7 5 3 1 2 36 Pin Botia Electrodes 36 34 32 30 28 26 24 rude 161412108 642 Connect the EcoFlexMEA24 directly to a 32 Channel Miniature Preamplifier The electrode field is at the bottom of the EcoFlexMEA24 Insert the EcoFlexMEA24 into the MPA32I with the electrode field up Technical Specifications EcoFlexMEA24 Tempe
99. ed charge is so high and the time constant for discharging so low 453 936 sec XAws 200 Ims gt 300 iThsweep Y Axis 1000 uV 4600 AR 8 99 8 9 9 0 0 1 0 0 a i 2 ist x E E The screen shot shows a prominent stimulus artifact on all channels followed by a response The stimulating electrode No 61 has been grounded The next pictures demonstrate the blanking feature On the left screen shot you see the stimulus artifacts on a non stimulating electrode without blanking On the right you see the same electrode and stimulation pattern but with blanking The stimulus artifacts have been completely avoided making it possible to detect signals shortly after the stimulus igi Blanking On 1859030sec XAws 500 ms gt 400 p eoo 28830sec Anis 500 ms gt 300 p 600 1887th sweep Y Avis 100 hv 10 0 1000 Sith sweep YAws 100 I 31000 1000 ST ne Bud uuu EE A ARCA 103 AEREA LAA ne GT AANA 53 MEA Manual 8 2 Capacitive Behavior of Stimulating Electrodes Regarding the generally used stimulus pulses stimulating electrodes behave as plate capacitors The charge cannot flow back to the stimulus generator due to the high output resistance and thus is kept in the electrode The electrode needs a quite long time to discharge itself after stimulation As a result stimulus artifacts interfere
100. el Systems MCS GmbH Product information is subject to change without notice multichannel systems 60 6wellIMEA Electrode Layout The letter digit code is the electrode identifier and refers to the position of the electrode in the 60 6wellMEA The specified amplifier pin numbers are the MEA System channel numbers that are used in MC_Rack program The pin numbers 32 61 84 67 38 and 15 are grounded 85 amp amp amp 6 8 69 6 62 1 amp re F9 fanp a7 aa 1 as a2 as a3 As a9 anp B7 64 B1 9 e likek S 8 8 8 8 8 amp amp amp amp amp amp amp amp amp amp amp amp G9 O 2012 Multi Channel Systems MCS GmbH amp amp amp amp amp amp amp amp amp amp amp 8 a w 2 8 2 a 2 2 a a a e1 e fnd ps ps bs os p2 pe p o4 07 fnd cs 69 69 69 amp 6 69 6 multichannel systems 60 6wellMEA Electrode layout inside each well Example Well A 33 44 4 2 3 83 ve amplifier pin numbers Overwiev MEA amplifier pin numbers digit and correspondent electrode identifier code letter digit inside each well 6 WOONDUNBWN Ui N N UJ oo N A N N o N A8 GND 32 61 84 67 38 15 Electrode identifier code refering to the position in the 6
101. embrane is suitable for all MEAs with glass ring A hydrophobic semipermeable membrane from Dupont that is selectively permeable to gases O CO but not to fluid and H O vapor keeps your culture clean and sterile preventing contaminations by airborne pathogens It also greatly reduces evaporation and thus prevents a dry out of the culture Reference e Reference Potter S M and DeMarse T B 2001 A new approach to neural cell culture for long term studies J Neurosci Methods 110 1 2 17 24 47 MEA Manual 6 3 MEA Culture Chamber with Lid Another possibility is to use a MEA culture chamber with lid available from Multi Channel Systems which is suitable for all MEAs with plastic ring and thread It can be adapted by inserting metal perfusion cannulas for setting up a continuous perfusion 6 4 Culture Chamber for 9 Well MEAs The culture chamber ring 9well CC for 256 9wellMEAs is suitable for the 9well macrolon quadrat available from Multi Channel Systems To use the macrolon quadrat in combination with the 9well CC as a culture chamber please insert a foil between macrolon quadrat and 9well CC ring 9well CC for 256 9wellMEA300 30iR ITO 6 5 Culture Chamber for 6 Well MEAs The 6well CC culture chamber is suitable for 6well macrolon rings on 60 6wellMEAs available from Multi Channel Systems The removable membrane cover for 60 6wellMEAs is available for triangle and round chamber rings 6well CC
102. er Several other MEA types and layouts that are dedicated to special applications are also available please see chapter MEA Types and Layouts for more details The biological sample can be positioned directly on the recording area the MEA serves as a culture and perfusion chamber A temperature controller controls the temperature in the culture chamber Various culture chambers are available for example with leak proof lid or with semipermeable seal An incubator is not necessarily required long term recordings in the MEA culture chamber are possible over several weeks or even months For cell or slice cultures MEAs have to be coated with standard procedures before use to improve the cell attachment and growth please read chapter MEA Coating Spike activity can be detected at distances of up to 100 um from a neuron in an acute brain slice Typically signal sources are within a radius of 30 uim around the electrode center The smaller the distance the higher are the extracellular signals The higher the spatial resolution the lower the numbers of units that are picked up by a single electrode that is the less effort has to be put into the spike sorting Multi Channel Systems provides MEAs with the highest spatial resolution in the market HighDenseMEAs have electrodes with a diameter of only 10 uim arranged in a distance of only 30 um center to center The challenge of manufacturing very small electrodes and at the same time keeping
103. er the electrode field there is a hole that makes it possible to access the electrode field from below The area around the electrodes is perforated to apply suction to the slice from below The total area of the holes averages 0 8 mm the diameters of the holes varies from 20 to 90 um Please read chapter Working with the USB MEA32 STIM4 Amplifier in USB MEA32 STIM4 manual or Setting up the MEA in the MEA2100 manual Using pMEAs Perforated MEAs have a robust ceramic carrier but the electrodes are embedded in polyimide foil Therefore they are heat stable to 50 C only and cannot be autoclaved Please do not use an ultrasonic bath for cleaning 21 MEA Manual 4 10 MEA with 6 Wells 60 6wellMEA 60 6wellMEA200 30iR Ti 60 6wellMEAs are MEA chips with six independent culture chambers separated by a macrolon ring Inside each well in between the marked two bars coming out of the circle in the middle of the MEA there is a field of nine electrodes with an internal reference electrode The electrode in the center of the MEA is for 60 6wellMEAs are developed for example for safety pharmacological screenings of drug induced QT prolongation Multi Channel System MCS GmbH provides a software solution for these experimental intentions the QT Screen Lite program The 60 6wellMEA allows running six experiments with identical surrounding conditions at once Two types of macrolon rings are available Rings with six triangular
104. erence electrode to the left side in the amplifier Otherwise the MEA layout will not match with the pin layout of the channel map in the data acquisition software Using standard MEAs Standard MEAs can be used for a wide variety of applications They are robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Generally they can be used for acute experiments as well as long term cultures 14 MEA Manual 4 3 High Density MEA 6 0HDMEA 60HDMEA30 10iR ITO 10 um electrodes are arranged in two recording fields with 5 x 6 electrodes each The interelectrode spacing is only 30 um center to center The very high electrode density of the two recording fields on a 60HDMEA is only possible by the special TiN electrode material and production process This MEA type is especially useful for applications where a high spatial resolution is critical for example for multitrode analysis For example the very high spatial resolution of the high density MEAs is very useful for recording from retina ganglia cells The double recording field can also be used for coculturing two slices each on one recording field The flat round electrodes are made of titanium nitride TiN Tracks and contact pads are made of transparent indium tin oxide ITO insulation material is silicon nitride 60HDMEA30 10iR ITO MEAs are available with internal reference electrode Using 60HDMEAs The same mater
105. ersibly damaged You could have a look at the electrodes under a microscope If they appear shiny golden the titanium nitride is gone and the electrode is irreversibly damaged Electrodes may be damaged without changing their visual appearance though Pick one of the bad channels after the other and ground it See the MEA amplifier s manual for more information on grounding channels In most cases only one of the electrodes that appear bad is actually defective and the other ones are only affected by the single defective electrode Ground as many electrodes as you need for a good general performance In the following example all defective electrodes have been grounded I Defective electrodes on ground 1036 000sec X Axis 1036th sweep Y Axis 1000 gt ms j o 200 400 200 400 200 400 x20 gt 200 w gt 400 200 oh oo F 5000 390 J 200 gp goo son guo sop goo so Pees ery spay ye 31 Lj GAl 1050 000 sec X Axis 1050th sweep Y Axis 1000 p ms o x20 y w gt oo p n m If too many electrodes are defective use a new MEA Contact pin is defective Please see the manual for the respective MEA amplifier 59 MEA Manual 9 4 Overall Noise Unsteady Baseline The baseline is unstable signals are jumping or drifting Possible causes Bath electrode is no
106. h 9 Wells for Use with USB MEA256 System 256 9wellMEA300 30iR ITO The 256 9wellMEA300 30iR ITO has 256 electrodes and has to be used with the USB MEA256 System Please refer to the USB MEA256 System manual for detailed information The dimension of the glass carrier is 49 x 49 x 1 mm as usual The MEAs with 9 wells are stable in a temperature range from 0 125 C The 256 9wellMEA contains 234 recording 18 stimulation or recording and four ground electrodes arranged in nine electrode blocks with a 6 x 5 layout grid for the recording electrodes two stimulation or recording electrodes and one reference electrode per each block They are embedded in a transparent glass substrate The contact to the amplifier is provided by a double ring of contact pads around the rim of the MEA The standard material for MEAs is also used for 256 9wellMEA The electrodes are from titanium nitride TiN with a silicon nitride SiN isolator and contact pads and tracks are made of transparent indium tin oxide ITO Using the 256 9wellMEA with macrolon quadrate you have nine separate culture chambers on one MEA for example for drug application in a screening experiment The spacing between the recording electrodes in the 6 x 5 grid averages 300 um between the electrodes The electrode diameter of 30 um results in an impedance of approximately 30 50 kO The dimension of the square stimulation electrode is 50 x 200 um Using 256 9wellMEAs
107. halograms EEG and microelectroretinograms ERG Typical applications in the cardiac field are Activation and excitation mapping measuring of the conduction velocity longterm characterizations of cell types especially stem cells culture pacing drug testing safety pharmacology studies monitoring of QT related prolongation and arrhythmias cocultures and disease implantation model For more information on published applications or procedures for biological preparations please see the application notes on the MCS web site http www multichannelsystems com applications html MEA Manual 3 2 MEA Design and Production A standard MEA biosensor has a square recording area of 700 um to 5 mm length In this area 60 electrodes are aligned in an 8 x 8 grid with interelectrode distances of 100 200 or 500 um Planar TiN titanium nitride electrodes are available in sizes of 10 20 and 30 um and three dimensional TiN electrodes have a diameter of gt 20 um at the base with a very fine tip Standard MEAs are useful for a wide variety of applications Different geometries match the anatomical properties of the preparation Most MEAs are available with a substrate integrated reference electrode replacing the silver pellet in the bath All electrodes can either be used for recording or for stimulation In principle MEA electrodes are not arranged symmetrical so the MEA chip has to be placed inside the amplifier in the recommended mann
108. has 256 electrodes and has to be used with the USB MEA256 System Please refer to the USB MEA256 System manual for detailed information The dimension of the glass carrier is 49 x 49 x 1 mm as usual The MEAs with 6 wells are stable in a temperature range from 0 125 C The 256 6wellMEA contains 252 recording 6 reference and four ground electrodes arranged in 6 electrode blocks with a 6 x 7 layout grid for the recording electrodes The reference electrode is around each block They are embedded in a transparent glass substrate The contact to the amplifier is provided by a double ring of contact pads around the rim of the MEA The standard material for MEAs is also used for 256 6wellMEA The electrodes are from titanium nitride TiN with a silicon nitride SiN isolator and contact pads and tracks are made of transparent indium tin oxide ITO Using the 256 6wellMEA with macrolon ring you have six separate culture chambers on one MEA for example for drug application in a screening experiment The spacing between the recording electrodes in the 6 x 7 grid averages 200 um between the electrodes The electrode diameter of 30 um results in an impedance of approximately 30 50 kQ Using 256 6wellMEAs The same material is used for standard MEAs and 256 6wellMEAs Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures 24 MEA Manual 4 13 MEA wit
109. haustrasse 21 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm PCB Printed circuit board Gold 100 um 700 um Planar Gold PCB Printed circuit board approx 30 kQ 8x8 59 1 internal reference electrode 2 dim MEA Default Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice 60EcoMEA Glass multichannel systems Low priced Microelectrode Array on Glass Base G GO 6 6 99 6 oe 66 aa Eee DO OOC0OO0OOO 00G eo e eG 6 69 amp amp 66 000 60EcoMEA on glass base with 8 x 8 standard electrode layout Technical Specifications 60EcoMEA Glass Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC_Rack Source layout in Data Source Setup Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS G
110. he signal collector SC2x32 leads the output channels of the second amplifier to channel numbers 33 to 64 Please read the SC2x32 data sheet MCS GmbH and only for the specified purpose Damage of the device and even injuries can Warning The device may only be used together with the MPA32I from Multi Channel Systems result from improper use Multi Channel Systems Fon 49 7121 9 09 250 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change without Germany www multichannelsystems com notice
111. ial is used for standard MEAs and high density MEAs Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures 15 MEA Manual 4 4 Hexa MEA 60HexaMEA 60HexaMEA Ti 60HexaMEA ITO 60HexaMEA40 1 OiR ITO o e 8 eooo o e e e 9 sere O10 220 230 um e 9 2ODOO D e eee e e 30 60 90 90 um 9 e LM Electrode layout for 60HexaMEA Ti and 60HexaMEA ITO Electrode layout for 60HexaMEAAO 10iR ITO HexaMEAs feature a hexagonal layout perfect for recording from retina The 60 electrodes of 60HexaMEA Ti or 60HexaMEA ITO are aligned in a special configuration with varying electrode diameters 10 20 30 uim and interelectrode distances see upper pictures The specific layout resembles ideally the regularity of the retina s architecture The density of neurons is more important in the center than in the peripheral This is matched by the density of electrodes on the MEA which is also higher in the center than in the peripheral The flat round electrodes are made of titanium nitride TiN Tracks are made of opaque Ti or transparent ITO and contact pads are made of TiN or ITO The insulation material is silicon nitride Electrodes in the center have a diameter of 10 um with an interelectrode distance of 20 um where the peripheral electrodes have a diameter of 20 um and 30 um This type of HexaMEA 60HexaMEA
112. ide Silicon nitride 500 nm PEVCD 250 400 KQ 2 x 5 x 6 59 1 internal reference electrode 2 dim MEA HighDenseMEA cmp HighDenseMEA L cmp HighDenseMEA R cmp Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice multichannel aA alam systems marins 6000000000000000 Electrode A3L C3L A4L ASL B4L B5L C4L C5L CIR C2R BIR B2R A1R A2R C3R A3R 12 pal Left electrode field Right electrode field AAR 82 3 ATL A5R 73 eeeo 060000 13 B2L 23 12 33 32 31 MEA pins 61 62 63 82 73 BAR 3 6 660606 666660 o 00000 00000 9 o poooo Heeeee rs 25 28 6 60666 606060600 o 0 6 0 6 37 I lt 4 26 FIL 30 um F5R 76 17 F2L F4R 7 The first letter of the electrode number code refers to the row number the digit is the column number and the second letter refers to the electrode field left or right of the 60ThinMEAS30 10iR Ti The specified MEA pin numbers are the channel numbers that are used in the MC_Rack program The electrode D1 of the left electrode field connected to channel 15 in MC_Rack is missing It is replaced by a big in
113. impedance of a flat round titanium nitride TiN electrode ranges between 30 and 400 kiloohms depending on the diameter The smaller an electrode the higher is the impedance On one hand lower impedance seems desirable but on the other hand a smaller electrode and interelectrode distance results in a higher spatial resolution Multi Channel Systems provides MEAs with TiN electrodes with sizes of 10 20 or 30 um and gold electrodes with 100 um which all show an excellent performance and low noise level The average noise level of 30 um and 10 um electrodes is less than 10 p V and 15 uV peak to peak respectively Gold electrodes EcoMEAs are only available with a low spatial resolution and are useful for medium throughput screening where costs are a limiting factor All planar TiN electrodes are positioned on a round pad with a diameter of 40 um If you like to check the electrodes with a light microscope you will need an upright microscope to see the MEA from above With an inverse microscope you are only able to see the bigger pad from below not the electrode itself The electrodes are embedded in a carrier material usually glass Standard tracks made of titanium Ti or indium tin oxide ITO are electrically isolated with silicon nitride SiN Standard contact pads are made of titanium nitride TiN or indium tin oxide ITO ITO contact pads and tracks are transparent for a perfect view of the specimen under the microscope 11
114. ing with nitrocellulose is a fast procedure that works with several cell types and tissues and that is also successful with slightly hydrophobic MEAs This method has the advantage that the cells stick well to the surface Nitrocellulose does not form a uniform layer on the MEA The coating leaves patches of nitrocellulose which serve as a glue for the tissue on the MEA surface The tissue is not likely to get detached even under severe mechanical disturbance by perfusion for example MEAs coated with nitrocellulose can be stored for a few days Nitrocellulose coating has to be removed after use Main advantages of this method are that nitrocellulose is cheap coating is fast and easy and it is also easily removed after use Note Nitrocellulose solutions cannot be stored for a longer period of time The solution forms a visible gelatinous precipitate after extended storage of at least half a year and will not produce satisfactory adhesive coatings anymore Prepare a fresh solution if there are visible precipitates Materials Protran or other standard nitrocellulose membrane Whatman PerkinElmer 100 Methanol Carl Roth GmbH Co KG UN No 1230 37 5 4 2 MEA Manual Nitrocellulose solution For preparing a stock solution dissolve a piece of 1 cm nitrocellulose membrane in 10 ml methanol Stock solutions may be stored at room temperature in polystyrene tubes For the working solution dilute the stock solution 10 1 with meth
115. iniature preamplifier MPA32I you do not need an adapter An additional connector on the side of the EcoFlexMEA36 can be used for connecting a silver pellet or a silver wire for grounding the bath Please read the data sheet EcoFlexMEA and the MPA32l manual for more information Using EcoFlexMEAs EcoFlexMEAs are usually connected to a head stage preamplifier that is connected to a filter amplifier or programmable gain amplifier see also the ME System product line of Multi Channel Systems EcoFlexMEAs can be directly connected to a 32 channel miniature preamplifier from Multi Channel Systems for in vivo experiments 32 MEA Manual EcoFlexMEA36 The EcoFlexMEA36 has 32 recording electrodes two internal reference electrodes and two ground electrodes in a 6 x 6 electrode grid The recording electrodes have a diameter of 50 um the distance between the electrodes from center to center is 300 um The electrodes contact pads and track material are made of pure gold EcoFlexMEA36 is stable at a temperature range from 0 C to 125 C and can be autoclaved The EcoFlexMEA36 can directly be connected to a standard 32 channel miniature preamplifier MPA32I you do not need an adapter The connector on the right side of the MEA see picture can be used for connecting a silver pellet or a silver wire for grounding the bath Please read the data sheet EcoFlexMEA36 and the MPA32l manual for more information EcoFlexMEA24 The EcoFlexMEA24 ha
116. lectrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm Glass Ti Titanium TiN Titanium nitride 50 x 50 um 200 um Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD 30 50 kQ 8x8 59 1 internal reference electrode iR 2 dim MEA Default Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH Fax 49 7121 9 09 25 11 info multichannelsystems com Product information is subject to change www multichannelsystems com without notice m multichannel 60SquareMEA200 501IR Ti systems Microelectrode Array with 60 square electrodes Standard electrode layout grid 8 x 8 wea pis G9 69 65 65 65 6 65 65 65 65 5 9 Electrode 4 21 32 31 44 41 42 52 51 54 61 62 71 33 33 22 22 x p Aa AAAA gt BBEBERHEBH e EB B3 BS Bs Es aS amp ARE pa AAR di 5 ss as 5s 5s rs Bs Bs fm Ge bel Es Es pe ag 9 p gy Bx p 7 AAA co o o N o N o 5 5 w w N N w Electrode
117. lectrodes The electrode diameter of 30 um results in an impedance of approximately 30 50 kQ The electrode diameter of 10 um results in an impedance of approximately 250 400 kO The dimension of the glass carrier is 49 x 49 x 1 mm as usual 256MEAs are stable in a temperature range from 0 125 C The 256MEA is only MEA type which is rotationally symmetric 256ThinMEAs are only 180 um thick ideally suited for high resolution imaging 256ThinMEAs are like standard MEAs but the electrodes are embedded in a very thin and delicate glass substrate on a robust ceramic carrier The thin glass allows the use of oil immersion objectives with a high numerical aperture Like 256MEAs 252 electrodes are arranged in a 16 x 16 layout grid with electrode diameter of 30 um and interelectrode distance of 200 um 256ThinMEA200 30 ITO Using 256MEAs The same material is used for standard MEAs and 256MEAs Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Using 256ThinMEAs 256ThinMEAs are heat stabilized and can be autoclaved They can also be coated with different procedures for cell and tissue cultures They should be handled with great care because of the thin and delicate recording area 23 MEA Manual 4 12 MEA with 6 Wells for Use with USB MEA256 System 256 6wellMEA200 30iR Ti nanna CIT rTTTTIII The 256 6wellMEA200 30iR ITO
118. lular recording and stimulation Cell lines or primary cell preparations are cultivated directly on the MEA Freshly prepared slices can be used for acute recordings or can be cultivated as organotypic cultures OTC on the MEA Recorded signals are amplified by a filter amplifier and sent to the data acquisition computer All MEAs except EcoFlex or FlexMEAs are only for use with MEA Systems or USB MEA Systems for extracellular recording from Multi Channel Systems MCS GmbH FlexMEAs may be used with components of ME Systems and USB ME Systems from Multi Channel Systems MCS GmbH EcoFlex and FlexMEAs are designed for use in in vitro or in vivo studies Please see setup manuals Setup USB MEA Systems and USB ME Systems for more information Several MEA geometries are provided for a wide variety of applications Almost all excitable or electrogenic cells and tissues can be used for extracellular recording in vitro for example central or peripheral neurons cardiac myocytes whole heart preparations or retina There are various applications for MEAs in the fields of neurobiology and cardiac electrophysiology Typical neurobiological applications are lon channel screening drug testing safety pharmacology studies current source density analysis paired pulse facilitation PPF long term potentiation LTP and depression LTD I O relationship of evoked responses circadian rhythm neuroregeneration developmental biology microencep
119. m Interelectrode distance centre to centre 200 um Electrode height Planar Electrode type TiN Titanium nitride Isolation type Polyimide foil 2610 isolator Electrode impedance 30 50 kO electrodes Electrode layout grid 12 x 12 Number of recording electrodes 120 Number of reference electrodes 4 internal reference electrodes iR Contact pads for reference electrodes connected to ground 4 MC Rack Source layout in Data Source Setup Configuration MEA MEA120 200 30 Rinse with distilled water Do not use ultrasonic bath These perforated MEAs are not heat stable and should not be autoclaved MEA perfusion chamber w o Without ring gr Glass ring ID 19 mm OD 24 mm pr Plastic ring without thread ID 26 5 mm OD 30 mm height 3 6 mm pr T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel systems 120pMEA200 30iR Ti c3 E5 D2 E4 E2 F4 F2 F5 G6 G1 G3 H1 H3 J1 J3 K2 c2 D3 D1 E3 E1 F3 F1 G5 G2 G4 H2 H4 J2 J4 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 B3 45 GND GND 76 K3 D4 44 77 13 c4 43 78 H5 SID me 868688 mee ee os 00000000 N e e 6 6 6 6 6000 m
120. mbH Aspenhaustrasse 21 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm Glass Gold 100 um 700 um Planar Gold SU 8 photoresist 1 2 um approx 30 KQ 8x8 59 1 internal reference electrode 2 dim MEA Default Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice i multichannel sete Mlle systems 60StimMEA200 30 Ti 60 electrodes Microelectrode Array with 16 additional stimulation electrodes Electrode layout Standard 8 x 8 grid For use with MEA STIM ADPT Adapter for MEAs with 16 additional stimulation electrodes and for MEA1060 amplifiers 2450 um 3 OOO 60000 O O O amp 6 6 660 9 6 amp 6 6 060230 Q Contact pad for stimulation adapter MEA STIM ADPT Diameter of contact pad 2 mm Distance between contact pads 4 1 mm Centre to centre 27 8 mm Technical Specifications 60 Electrode MEA with 16 additional Stimulation Electrodes Temperature compatibility 0 125 C Dimension W x D x H 49 mm x 49 mm x 1 mm Base material Glass Track material Indium tin oxide ITO or Titanium Ti
121. mg ml pH 3 0 Cellmatrix Type I A Nitta Gelatin Inc Preparation buffer 200 mM HEPES in 0 08 N NaOH Collagen solution Add 1 ml of 10 x DMEM F 12 medium to 8 ml Cellmatrix Type I A and stir gently Add 1 ml of preparation buffer and stir gently Incubate the mixture at 4 C for 30 min to remove any air bubbles if necessary Store at 4 C until use Procedure 1 Sterilize the MEA before the coating with collagen and perform all following steps under sterile conditions 2 Incubate the MEA at 4 C for at least 1h 3 Fill the MEA with collagen solution until the bottom of the culture chamber is completely covered Immediately remove the collagen solution with a glass pipette The solution can be reused 4 Incubate the MEA in a CO incubator for 30 min Rinse the MEA with sterile distilled water Fill the MEA with culture medium and keep it sterile in a CO incubator until use for up to one week Check for contaminations before use 42 MEA Manual 5 5 Cleaning of used MEAs 5 5 1 5 5 2 5 5 3 5 5 4 General Recommendations for Cleaning MEAs The cleaning procedure depends on the kind of coating and on the kind of biological preparation In the following a few general considerations are listed If you have recorded from an acute slice without coating you can simply rinse the MEA with distilled water and the MEA should be fine If necessary the MEA can then be cleaned with any cleaning agent for example
122. mm Please note Not all rings can be placed on all MEAs Please check exact order description 49 7 MEA Manual Recording with MEAs 7 1 Mounting the MEA 7 1 1 Cleaning the Contact Pads You should always clean the contact pads with alcohol before placing it into the MEA amplifier Even if you do not see any contaminations a very thin grease layer from touching the pads with bare fingers for example may be present and results in a bad contact between the pads and the amplifier pins A bad contact will result in an increased noise level on the affected channel This is the most prominent handling error Carefully wipe the MEA contact pads with a clean and soft tissue moistened with pure alcohol Positioning the MEA Important MEAs are not symmetrical That is why the writing for example NMI LEITER MEA type on the MEA chip should be on the right side viewed from the front with the sockets of the amplifier in the back MEAs with one big internal reference electrode should be placed with reference electrode to the left side in the amplifier Otherwise the MEA layout will not match with the pin layout of the channel map in the data acquisition program When placing a special MEA into the amplifier for example 120MEA HighDense MEA 4Q1000 MEA or HexaMEA please make sure that the orientation of the MEA is correct respectively Grounding the Bath Make sure that the bath is connected to the amplifier s ground Attach
123. multichannel systems Microelectrode Array MEA Manual Information in this document is subject to change without notice No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH While every precaution has been taken in the preparation of this document the publisher and the author assume no responsibility for errors or omissions or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document 2015 Multi Channel Systems MCS GmbH All rights reserved Printed 28 05 2015 Multi Channel Systems MCS GmbH AspenhaustraBe 21 72770 Reutlingen Germany Fon 49 71 21 90925 0 Fax 49 71 21 90 92 5 11 info multichannelsystems com www multichannelsystems com Microsoft and Windows are registered trademarks of Microsoft Corporation Products that are referred to in this document may be either trademarks and or registered trademarks of their respective holders and should be noted as such The publisher and the author make no claim to these trademarks 1 1 2 1 2 2 23 3 1 3 2 3 3 4 1 4 2 4 3 4 4 4 5 4 6 4 7 4 8 4 9 4 10 4 11 4 12 4 13 4 14 4 15 4 16 4 17 4 18
124. n 2 No 52 D24 Conn 4 No 34 E24 Conn 3 No 62 F24 Conn 2 No 48 D25 Conn 4 No 31 E25 Conn 3 No 59 F25 Conn 2 No 45 D31 Conn 4 No 44 E31 Conn 1 No 2 F31 Conn 2 No 58 D32 Conn 4 No 41 E32 Conn 4 No 63 F32 Conn 2 No 55 D33 Conn 4 No 36 E33 Conn 3 No 63 F33 Conn 2 No 50 D34 Conn 4 No 32 E34 Conn 3 No 60 F34 Conn 2 No 46 D35 Conn 4 No 29 E35 Conn 3 No 57 F35 Conn 2 No 43 D41 Conn 4 No 43 E41 Conn 1 No 57 F41 Conn 2 No 29 D42 Conn 4 No 46 E42 Conn 1 No 60 F42 Conn 2 No 32 D43 Conn 4 No 50 E43 Conn 1 No 63 F43 Conn 2 No 36 D44 Conn 4 No 55 E44 Conn 2 No 63 F44 Conn 2 No 41 D45 Conn 4 No 58 E45 Conn 3 No 2 F45 Conn 2 No 44 D51 Conn 4 No 45 E51 Conn 1 No 59 F51 Conn 2 No 31 D52 Conn 4 No 48 E52 Conn 1 No 62 F52 Conn 2 No 34 D53 Conn 4 No 52 E53 Conn 2 No 60 F53 Conn 2 No 37 D54 Conn 4 No 53 E54 Conn 2 No 62 F54 Conn 2 No 39 D55 Conn 4 No 56 E55 Conn 2 No 61 F55 Conn 2 No 42 D62 Conn 4 No 47 E62 Conn 1 No 61 F62 Conn 2 No 33 D63 Conn 4 No 49 E63 Conn 2 No 57 F63 Conn 2 No 38 D64 Conn 4 No 54 E64 Conn 2 No 59 F64 Conn 2 No 40 DS2 Conn 4 No 51 ES2 Conn 2 No 2 FS2 Conn 2 No 35 Electrode ID Electrode Stimulation Electrode Stimulation Electrode Stimulation and number aD Socket ID Socket ID Socket E Gs1 Conn 4 No 24 HS1 Conn 3 No 52 Js1 Conn 3 No 24 of stimulation G12 Conn 4 No 26 H12 Conn 3 No 54 312 Conn 3 No 26
125. n 3 No 36 Js2 Conn 3 No 7 Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems Stim Elect Sprin Hard Stim Elect Spri Hard Stim Elec Spri Hard Stim Elect Spri ard Sock rode g ware Sock rode ng war Sock trod ng war Sock rode ng are et ID Con ID et ID Con eID et eID Con eID et ID Con D 1 A35 2 226 1 C35 35 112 1 J41 68 96 1 G41 101 240 2 E31 1 196 2 ES2 34 82 2 E45 67 66 2 ES1 100 210 3 A25 3 158 3 C25 36 44 3 J51 69 28 3 G51 102 172 4 A34 133 130 4 C34 163 13 4 J42 193 126 4 G42 223 141 5 A14 4 223 5 C14 37 109 5 Je2 70 64 5 G62 103 241 6 A24 134 193 6 C24 164 79 6 J52 194 214 6 G52 224 211 7 AS1 5 155 7 C23 38 41 7 JS2 71 63 7 G53 104 173 8 A33 135 253 8 CS1 165 14 8 J43 195 217 8 GS2 225 142 9 A23 6 224 9 C33 39 110 9 J53 72 244 9 G43 105 242 10 A13 136 194 10 C13 166 80 10 J63 196 216 10 G63 226 212 11 A22 7 156 11 C22 40 42 11 J54 73 247 11 G54 106 174 12 A12 137 254 1
126. n 1l distilled water at 80 C Adjust pH to 8 4 with 1 N HCI 38 5 4 3 MEA Manual PEI stock solution 0 05 0 1 96 PEI dissolved in borate buffer Laminin solution 20 pg ml laminin in plating medium Procedure Note It is necessary to thoroughly rinse off unbound PEI from the plates before use as dried PEI is toxic Pipette 500 ul PEI solution onto the MEA The recording field should be completely covered Incubate at RT for 1 h or at 4 C over night Remove the PEI solution and thoroughly rinse 4 x with distilled water Air dry the MEA Sterilize with UV light for at least 1 h after coating Place a drop of sterile laminin solution onto the MEA and incubate for 30 min Aspirate do not rinse and directly seed your cells Alternatively mix the cells with laminin solution before plating Literature Ulrich Egert Thomas Meyer 2004 Heart on a Chip Extracellular multielectrode recordings from cardiac myocytes in vitro Methods in Cardiovascular Research S Dhein and M Delmar eds Lelong IH et al 1992 J Neurosci Res 32 562 568 Coating with Polyornithine plus Laminin Poly D lysine can be used as an alternative for polyornithine Materials Polyornithine Laminin 1mg ml Sigma Aldrich Inc L2020 Polyornithine solution 500 pg ml polyornithine in distilled water Laminin solution 5 pg ml laminin in plating medium or PBS phosphate buffered saline 39 5 4 4
127. n Connector Socket 1 Stimulation Connector Socket 2 Stimulation Connector Socket 3 Stimulation Connector Socket 4 Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware Electrode Stim Spring Hardware ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID ID Socket Contact ID A2 2 1 196 19 60 191 125 C15 61 98 239 A10 25 113 236 B1 4 133 130 K7 7 38 41 C16 62 222 209 A11 22 232 205 B2 1 2 226 K8 34 178 74 D13 63 99 171 A12 15 108 169 C1 5 4 223 K9 33 51 104 D14 57 96 184 A13 12 227 143 c2 6 134 193 L10 56 189 2 D15 58 220 213 A14 5 103 241 c3 3 3 158 L11 59 64 30 D16 55 95 181 A15 4 223 141 D1 12 137 254 L7 11 40 42 E13 53 94 182 A3 62 252 195 D2 9 6 224 L8 31 50 35 E14 54 218 243 A4 55 128 159 D3 10 136 194 L9 36 179 5 E15 51 93 179 A5 52 247 131 E1 15 9 55 M10 46 184 69 E16 52 217 147 A6 45 123 229 E2 16 139 25 M11 49 59 100 F11 59 97 183 A7 42 242 202 E3 13 8 123 M5 8 165 14 F12 49 92 180 A8 35 118 166 E4 14 138 93 M6 18 170 78 F13 50 216 145 A9 32 237 138 E5 8 135 253 M7 21 45 105 F14 47 91 177 B10 26 234 206 F1 22 142 91 M8 28 175 7 F15 48 215 191 B11 19 110 170 F2 19 11 56 M9 39 54 33 F16 45 90 178 B12 16 229 144 F3 20 141 26 N10 43 56 34 G11 56 219 245 B13 9 105 242 F4 17 10 124 N11 50 186 70 G12 46 214 192 B14 6 224 211 F5 18 140 94 N12 53 61 97 G13 43 89 175 B15 1 101 240 G1 25 14 122 N13 63 66 27 G14 44 213 189 B16 2 100 210 G2 26 144 92 N5 14 168 77 G15 41
128. n coating is acceptable in the planned experiments there is another quick and simple way to render the surface hydrophilic Sterilize the MEAs as described below Place approximately 1 ml of a concentrated sterile protein solution for example albumin fetal calf serum or similar onto the culture region for about 30 min Wash the culture chamber thoroughly with sterile water afterwards The MEA can then be directly used for cell culture 35 MEA Manual Preculturing Another pragmatic method is to coat the hydrophobic MEAs and to plate the cell cultures on the MEA and let it grow for some days up to weeks until the cells have transformed the surface so that it is sufficiently hydrophilic The preculture will generally show very bad growth and viability and needs to be discarded before plating the culture that will be used for experiments Please note that the MEA and the electrode performance may suffer under cell culturing Therefore the above mentioned methods are preferable 5 2 Sterilization A 5 2 1 5 2 2 5 2 3 5 2 4 Sterilization of MEAs is not necessary for acute slices Glass MEAs with TIN electrodes and EcoMEAs with gold electrodes can be sterilized with standard methods for cell culture materials using either rinsing with 70 alcohol UV light about half an hour depending on the intensity vapor autoclavation or dry heat sterilization up to a temperature maximum of 125 C Warning Do no
129. ng 5 4 1 Coating of MEAs with various materials is used for improving the attachment and growth of cell cultures or cultured slices Coating is generally not required for recordings from acute slices Coating of MEAs has the same purpose than coating of other culture dishes Therefore you can generally use the same standard protocols that you have established for coating culture dishes for your cell cultures provided that the involved chemicals are not aggressive and damage the electrodes see recommendations for the various MEA types In the following some standard coating procedures are shortly described You should try out which coating procedure proves best for your application The listed materials are only recommendations you may use any equivalent equipment Most coatings are stable for several uses of the MEA and do not have to be removed after use except nitrocellulose Please note that the materials and procedures described in the following are only a summary of published information from other sources see references or from personal communications with MEA users and has the intention of helping users finding the appropriate information for setting up their experiments Multi Channel Systems MCS GmbH has not tested or verified this information and therefore cannot guarantee that the information is correct Please refer to the referenced literature for planning and executing any experiments Coating with Nitrocellulose Coat
130. ns 21 51 H6 e Gs 33 44 43 53 di 9 9 e i 93 r5 12 32 42 52 62 FA 23 r 60606020 F5 72 OO s j a i ee Q amp 25 35 65 E e 66666060 di 6 9 9000 a 26 D2 17 37 47 57 67 87 D5 9 6060606026 56 55 66 e 06006000 2 48 58 68 78 B1 A1 C2 A2 B2 B3 A3 C3 C4 A4 B4 B5 A5 C5 A6 B6 The letter of the electrode number code refers to the row number and the digit is the column number The specified MEA amplifier pin numbers are the MEA System channel numbers that are used in the MC Rack program The substrate integrated reference electrode REF is connected to pin 15 of the MEA amplifier Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60HighDenseMEA 60HD30 10iR ITO multichannel systems 60 High Density Microelectrode Array with Internal Reference Electrode Technical Specifications 60HighDenseMEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Distance between electrode fields Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of referenc
131. o 49 F46 Conn 4 No 29 D71 Conn 3 No 8 E75 Conn 3 No 50 F55 Conn 4 No 30 D63 Conn 3 No 9 E54 Conn 3 No 51 F36 Conn 4 No 31 D72 Conn 3 No 10 E76 Conn 3 No 52 F45 Conn 4 No 32 D53 Conn 3 No 11 E55 Conn 3 No 53 F26 Conn 4 No 33 D73 Conn 3 No 12 E66 Conn 3 No 54 F35 Conn 4 No 34 D64 Conn 3 No 13 E45 Conn 3 No 55 F16 Conn 4 No 35 D74 Conn 3 No 14 E56 Conn 3 No 56 F44 Conn 4 No 36 D65 Conn 3 No 15 E44 Conn 3 No 57 F15 Conn 4 No 37 D75 Conn 3 No 16 E46 Conn 3 No 58 F25 Conn 4 No 38 D54 Conn 3 No 17 E35 Conn 3 No 59 F14 Conn 4 No 39 D76 Conn 3 No 18 E36 Conn 3 No 60 F24 Conn 4 No 40 D55 Conn 3 No 19 E34 Conn 3 No 61 F13 Conn 4 No 41 D66 Conn 3 No 20 E26 Conn 3 No 62 F34 Conn 4 No 42 D45 Conn 3 No 21 E16 Conn 3 No 63 F12 Conn 4 No 43 D56 Conn 4 No 22 E15 Conn 4 No 1 F23 Conn 4 No 44 D44 Conn 4 No 23 E25 Conn 4 No 2 F11 Conn 4 No 45 D46 Conn 4 No 24 E14 Conn 4 No 3 F22 Conn 4 No 46 D35 Conn 4 No 25 E24 Conn 4 No 4 F21 Conn 4 No 47 D36 Conn 4 No 26 E13 Conn 4 No 5 F33 Conn 4 No 48 D34 Conn 4 No 27 E33 Conn 4 No 6 F31 Conn 4 No 49 D26 Conn 4 No 28 E12 Conn 4 No 7 F32 Conn 4 No 50 D25 Conn 4 No 29 E23 Conn 4 No 8 F41 Conn 4 No 51 D16 Conn 4 No 30 E11 Conn 4 No 9 F43 Conn 4 No 52 D24 Conn 4 No 31 E22 Conn 4 No 10 F51 Conn 4 No 53 D15 Conn 4 No 32 E21 Conn 4 No 11 F42 Conn 4 No 54 D23 Conn 4 No 33 E43 Conn 4 No
132. otice 256 6wellMEA 256 6wellMEA200 30iR ITO rcr 256 6wellMEA200 30iR ITO tcr 6 Well Microelectrode Array for use with USB MEA256 System The MEA is not symmetrical and has to be inserted into the amplifier with well A on top as shown in the picture Technical Specifications 256 6wellMEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Contact pads for reference electrodes connected to ground Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany multichannel systems e Connector 4 Z Jo Oe UuuOyD o m m Li B Li LB Li B Li D Li B LB B Li g B Li B LB B 0 B Li Bi mi B Li D Li B m Oo 0 LCILICICIC IE JY CY JC 3C IC IC JE JC IC YE JC IC YC JC YE C CY IC ICT Qe 0 125 C 49 mm x 49 mm x 1 mm Glass Titanium nitride TIN and Titanium Ti 30 um 200 um Planar Titanium nitride TiN Silicon nitride SiN 30 50 kQ 6 x 7 x 6 electrodes 252 42 recording electrodes in each well 6 one internal
133. p amp 600eoesoeO0ee60089 Le Re HH amp amp 6 amp 9 amp amp P 6 9 HG BAH 22 es po 4 Go Gb amp o amp o a do o Ko Go im m Gn MH Mo osp 2 GARAHAAHAHAGHDH GAG HHA anew amp amp m momomimeieiiemeomese D oe amp amp do d amp Gb do o o 9 HH AAA se sin amp amp da m Ey em es m mmm miam 00 25 4 amp amp de Gb Eb F5 Gb de o G o m o Ob b H se 6 HF esos 2s E12 Gto 018 Pra B14 A14 Ut 19 Ai 014 P14 R14 B16 B15 N13 GND e00000900000000000000000000000000 GAD 6i A Gis Gi Eis Eis D ES di GD di di di di O GH is io Kis io BAH GD NTS MD ndo wa vio GS dio i The letter digit code is the electrode identifier and refers to the position of the electrode in the 16 x 16 layout grid The layout of the letter digit code for the four connectors of the USB MEA256 amplifier is shown To correlate the pin layout of the connectors please see the table on the next page Multi Channel Systems Fon 49 7121 9 09 25 O O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change German y www multichannelsystems com without notice l multichannel 256ThinMEA systems Stimulatio
134. pendix you find safe charge injection limit curves that document maximum current and stimulus durations for standard TiN electrodes Please note that these curves document the limits Stimulus pulses should be kept safely below these limits The safe charge injection limit of platinum 0 4 mC cm is much smaller than for TiN 23 mC cm This fact results in a considerably lower charge that you can inject into the electrode before faradic reactions occur that will lead to electrolysis of the electrode Please note that when using voltage driven stimulation the current flow to the electrode depends on the electrode impedance The lower the impedance the higher is the current Please make sure to obey the safe charge injection limits always Generally TiN electrodes have lower impedances than Pt electrodes and larger electrodes also have lower impedances than smaller When using TIN electrodes it is extremely important to not charge the electrodes positively as this will lead to electrolysis This is not an issue for Pt electrodes Therefore when using voltage driven stimulation itis important to apply negative voltages only Positive voltages will shortly charge the electrodes positively even though the electrode is discharged at the end of the pulse As a consequence biphasic voltage driven stimulation is not recommended When using current stimulation it is required to use biphasic stimulation and to apply the negative phase first to avoi
135. pre coated MEA with laminin solution at 4 C over night Aspirate the laminin solution and directly plate the cells Literature Goslin et al 1988 Nature 336 672 674 Maeda et al 1995 J Neurosci 15 6834 6845 Gross et al 1997 Biosensors amp Bioelectronics 12 373 393 40 MEA Manual 5 4 5 Coating with Poly D Lysine plus Fibronectin 5 4 6 This coating method is used for example for culturing dissociated suprachiasmatic nucleus SCN neurons on standard 60MEA200 30 It is very stable and therefore especially useful for long term cultures Materials Poly D lysine 5 mg 10 mL 0 05 w v stock solution Sigma Aldrich Inc P7280 Fibronectin BD BioCoat Fibronectin Cellware BD Biosciences Fibronectin solution Prepare a stock solution of 25 ug ml fibronectin in distilled water or PBS phosphate buffered saline and store it at 4 C Poly D Lysine plus fibronectin solution Prepare a 0 01 96 w v poly D lysine solution and add fibronectin 1 1 resulting in a final concentration of 12 5 ug ml Procedure Pipette 10 ul of the poly D lysine plus fibronectin solution onto the recording field Pipette about 50 ul of sterile distilled water near the rim of the culture chamber Incubate for 1 h in an incubator set to 35 C 65 relative humidity 9 96 O 5 96 CO or 37 C 100 96 humidity 5 96 CO To avoid a dry out of the liquid place the MEA in a big Petri dish with lid on Rinse 2 x with
136. preamplifier MPA32I Flex Please read the data sheet FlexMEA36 and the MPA321 Flex manual for more information FlexMEA72 The FlexMEA72 has 64 recording electrodes plus four internal reference electrodes and four ground electrodes in a 8 x 9 electrodes grid The titanium nitride electrodes have a diameter of 100 uim and the distance between the electrodes is either 625 um or 750 um The polyimide foil is perforated with holes of 100 um diameter ensuring optimal tissue contact When using the Flex MEA72 together with two standard 32 channel miniature preamplifier MPA32I you need the ADPT FM 72 adapter to connect the FlexMEA72 to two standard MPA32Is Please read the data sheet FlexMEA72 or ADPT FM 72 and the MPA321 manual for more information 31 MEA Manual 4 19 EcoFlexMEA EcoFlexMEAs are made of flexible polyimide Kapton They are less flexible as FlexMEAs but therefore more robust in handling and sterilization With a thickness of 50 um and low weight the EcoFlexMEA is perfect for in vivo and specific in vitro applications respectively The EcoFlexMEA is available with 24 or 36 electrodes two internal reference electrodes and two ground electrodes Custom layouts can be provided on request The electrodes contact pads and track material are made of pure gold EcoFlexMEAs are stable at a temperature range from O C to 125 C and can be autoclaved The EcoFlexMEA can directly be connected to a standard 32 channel m
137. r in the connectors 1 to 4 Spring Contact Spring contacts in the lid of the amplifier Hardware ID Hardware channel ID of MC_Rack hardware channels using the linear layout Electrode ID Electrode ID of the MEA electrode in the 16 x 16 layout grid Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com 2013 Multi Channel Systems MCS GmbH Product information is subject to change without notice 256ThinMEA 256ThinMEA200 30iR ITO A Thin microelectrode array with 16 x 16 layout The electrodes are embedded in a very thin glass substrate on a robust ceramic carrier Contact pads and tracks are made from transparent ITO for high resolution imaging e Connector 4 multichannel systems Connector 1 4 ABCDEFGH I KLMNOPR gt e Z 10120uuo23 ei v ABCDEFGHIKLMNOPR vv Connector 3 7 Technical Specifications 256ThinMEA200 30iR ITO Temperature compatibility Dimension W x D x H Thickness of the glass part Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Contact pads for reference electrodes
138. rature compatibility 10 125 C Dimension W x D 36 mm x 25 mm Thickness region of electrodes 50 um Base material Polyimide Kapton Contact pads and leads Gold Electrode diameter 80 um Interelectrode distance centre to centre 300 um Electrode heights Planar Electrode type Gold Isolation type Polyimide Kapton Electrode impedance Approximately 30 50 kQ Electrode layout grid 10x2 4 Number of recording electrodes 23 Number of reference electrodes 2 internal reference electrodes Number of ground electrodes 2 ground electrodes MC_Rack Version 4 1 1 and higher Data Source Setup Source Layout Configuration Total Number of Channels 64 Amplifier FA32I S or FA64I S MEA EcoFlexMEA24 Channel map Display Layout tab gt Default Map EcoFlexMEA24 cmp Cleaning Rinse with distilled water EcoFlexMEAs are heat stable and can be autoclaved Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 25 mm multichannel systems EcoFlexMEA24 Electrode Layout 12 mm Electrode field on the bottom of the EcoFlexMEA24 H 9 mm E o w m O co N 36 mm EcoFlexMEA24 OUT MPA32I IN MC Rack Channel Map Pin Electrode MC Rack Channel Pin 1 GND Top Pin 2 Ref Stim Bottom A2 A3 Pin 3 A3 1 Pin 4 D3 2
139. re beside Technical Specifications 9 Well MEA Temperature compatibility Dimension W x D x H Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Contact pads for reference electrodes connected to ground 0 125 C 49 mm x 49 mm x 1 mm Glass Indium tin oxide ITO 30 um recording 50 x 200 um stimulation 300 um recording 500 um stimulation Planar Titanium nitride TiN Silicon nitride SiN 30 50 kQ 6 x 5 recording 2 stimulation electrodes in each well 252 26 recording electrodes in each well 9 1 internal reference electrode iR in each well 4 Source layout in Data Source Setup Configuration Channel map 9 well 256MEA cmp MEA perfusion chamber w o Without Macrolon quadrat mq Macrolon quadrat with 9 wells ID 6 5 x 6 5 mm of each well OD 24 x 24 mm of all wells height 9 mm Volumetric capacity of each well minimum 250 ul Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen infoGmultichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems Macrolon Quadrat
140. reference electrode iR around each well 4 the reference electrodes are connected to 2 pads only Configuration 256 6wellMEA cmp tcr Triangle chamber ring with 6 wells OD 30 mm ID 27 mm height 10 mm of each well Volumetric capacity of each well minimum 700 yl rcr Round chamber ring with 6 wells OD 30 mm ID of the wells 7 mm height 10 mm of each well Volumetric capacity of each well minimum 500 yl Fon 49 7121 9 09 25 O 2014 Multi Channel Systems MCS GmbH Fax 49 7121 9 09 25 11 info multichannelsystems com Product information is subject to change www multichannelsystems com without notice multichannel systems 256 6wellMEA FTA A44 A35 A16 A18 A14 A18 A12 AT A21 A31A41 A51AG1 AT A72 AT3 ATA ATS ATG ASS A45 B14 B13 812 B11 B21 31 B41 B51 B61 GND Ado Ko A26 A25 A24 634 A23 622 33 A32 A42 A43 A52 ABB iAG2 AGS GA ABA AGB AGE ASG G24 634 G23 622 833 632 B42 B43 852 653 ET GND F54 872 B62 Ho E oen Sa To ee 0092909 ee Faa F51 eecececo ee o0 2292266 oe eo LELLE 646 B45 4 900909090 ES LT 0000009 pesos al ecectose o0 ee 2000000 2200006 ee eo 2900000 20909000 ee seis geesese Ss 22 2090000 B sccoceo ps F45 F36 661 65 F55 F46 2990099 E C 9309000 D 65 F3 20C000 ooccoocec ee FIS Fes 000600900 2000000 ee ee ococcoc oooccco ee e9 20990000 2000000 ee oo 2009990 ESOS ALLII ee ee coccooco Pe o0 ccoococ ee HH coo0ccoco oe 20 o0coocoo 26 HM
141. rientation when looking from the front into the opened headstage The small arrow near to the contact pads of the 120MEA faces upwards Under microscope control you can read the markers A1 and A12 M1 and M12 as shown on the diagram Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 Multi Channel Systems 2013 Multi Channel Systems MCS GmbH MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Product information is subject to change without notice info multichannelsystems com www multichannelsystems com multichannel systems 120MEA 120MEA200 30iR Ti 120MEA100 30iR ITO Electrode Layout Table Hardware ID and Electrode ID HW ID EL ID HW ID EL ID HW ID EL ID HW ID EL ID 120 G8 90 H6 60 F5 30 E7 119 G12 89 M6 59 F1 29 A7 118 G11 88 L6 58 F2 28 B7 117 G10 87 K6 57 F3 27 C7 116 G9 86 J6 56 F4 26 D7 115 H12 85 M5 55 E1 25 A8 114 H11 84 L5 54 E2 24 B8 113 H10 83 K5 53 E3 23 C8 112 H9 82 J5 52 E4 22 D8 111 J12 81 M4 51 D1 21 A9 110 J11 80 L4 50 D2 20 B9 109 J10 79 K4 49 D3 19 c9 108 H8 78 H5 48 E5 18 E8 107 K11 77 L3 47 C2 17 B10 106 K10 76 K3 46 C3 16 C10 105 L10 75 K2 45 B3 15 C11 104 J9 74 J4 44 D4 14 D9 103 K9 73 J3 43 CA 13 D10 102 L9 72 J2 42 B4 12 D11 101 M9 71 J1 41 A4 11 D12 100 J8 70 H4 40 D5 10 E9 99 K8 69 H3 39 C5 9 E10 98 L8 68 H2 38 B5 8 E11 97 M8 67 H1 37 A5 7 E12 96 J7 66 G4 36 D6 6 F9 95 K7 65 G3 35 C6 5 F10 94 L7 64 G2 34 B6 4 F11 93 M7 63 G1 33 A6 3 F12 92 H7 62 G5 32 E6 2 F8 91 G7 61 G6 31 F6 1 F7
142. rning It is absolutely necessary to rinse the MEAs thoroughly with distilled water after treatment with detergent particularly when using Terg A Zyme before heat sterilization dry heat sterilization is not recommended Otherwise the potential rests of the detergent may burn into the glass carrier of the MEA and may destroy the electrodes 45 Recording with MEAs 6 Culture Chamber and Ring Options You have several options regarding culture chamber interface rings without ring glass ring plastic ring without and with thread and culture chambers which are especially useful for long term cultures or experiments For more details or pricing information please ask your local retailer 6 1 MEA2100 CO2 C The MEA2100 CO2 C is a climate chamber for MEA2100 Systems Connect the chamber via magnetic forces on the lid of a MEA2100 headstage to create a 5 96 CO2 atmosphere humid or non humid around the biological probe Connect a tube with an inner diameter of 2 3 mm to the tube connector 6 2 Sealed MEA Culture Dish In order to allow long term cultivation and recording Multi Channel Systems recommends the use of teflon membranes fluorinated ethylene propylene 12 5 microns thick developed by Potter and DeMarse 2001 The ALA MEA MEM membrane is produced in license by ALA Scientific Instruments Inc and distributed via the worldwide network of MCS distributors The sealed MEA culture chamber with transparent semipermeable m
143. s 24 recording electrodes two internal reference electrodes and two ground electrodes in a 2 x 10 4 electrode grid The recording electrodes have a diameter of 80 uim the distance between the electrodes from center to center is 300 um The electrodes contact pads and track material are made of pure gold EcoFlexMEA36 is stable at a temperature range from 0 C to 125 C and can be autoclaved The EcoFlexMEA36 can directly be connected to a standard 32 channel miniature preamplifier MPA32I you do not need an adapter Please read the data sheet EcoFlexMEA24 and the MPA321 manual for more information 33 MEA Manual 4 20 MEA Signal Generator 60MEA SG 60MEA SG The 60MEA Signal Generator is a convenient tool for MEA Systems first time users It can replace a MEA for learning and or teaching purposes The device has the same dimensions and contact pad layout as a 60 channel MEA chip and is compatible with all MEA1060 amplifier types and with the MEA2100 System with 60 channel headstage MEA2100 HS60 or MEA2100 HS2x60 The MEA SG produces sine waves or replays a variety of biological signals These signals are fed into the MEA amplifier as analog signals With this artificial data you are able to test the functionality of the hardware and software system without the need for a biological sample on a real MEA Please use the 256MEA SG for the USB MEA256 System and the 120MEA SG for MEA2100 System connected to a headstage with 120
144. s or Strange Signal Behavior 61 10 Appendix 63 10 1 Contact Information 63 10 2 Safe Charge Injection Limits 64 10 3 Data Sheets 65 1 MEA Manual Introduction 1 1 About this Manual The MEA manual comprises all important information about the microelectrode arrays MEA for use with USB MEA or ME Systems from Multi Channel Systems The MEA manual focuses on general information on the MEA design use and handling and more specific information on different MEA types It also includes recommendations on sterilization coating and cleaning procedures from scientifical papers or from recommendations of other MEA users For more details on issues that refer to the amplifier like grounding or mounting the MEA please refer to the manual for the MEA amplifier you use You will find more information about the MEA System and its components in general especially the data acquisition card in the MEA System manual For more details on the data acquisition and analysis program Multi Channel Suite or MC_Rack please refer to the manuals It is assumed that you have already a basic understanding of technical terms No special skills are required to read this manual The components and also the manual are part of an ongoing developmental process Please understand that the provided documentation is not always up to date Please check the MCS Web site www multichannelsystems com from time to time for downloading up to date manuals Those p
145. s subject to change without notice multichannel 256MEA systems A261 02E5D3D1E4E2 F5 F3 F1G4G2H5H3 H1 H7 16 12 14 KY K8 KS L2L4 M1 M3 K6 N2 18 O1GND B263 01G87D2G6 E3E1 FA4F2G5 G3 G1 H4 H2 H6 17 11 18 15 K2 KA L1 L8 L5 M2 MA N1N3 L6 O2 N4 GND D4 BD A8 B3 03 R2 He F6 RS P3 8404 K7 M5 oe 66660600600006606 ee M e amp 6 668060600069 6060660628 cs ns ee 8 amp 66060688660066002882G0666286268628 d us amp amp 6eo0090000t90 Pece 4 S86 GGG AEH HH BOGE EE mt bees 4 amp amp 6 6 E GP de o Ko s No Eo fo do are 22 0000000000000000 Sa SS amp amp c ege 0oe0063 090 n 2 amp amp 6 6896690000200 20 E D amp do Gy Gn a A o do m Ko o du v Oh A do oss 009000000000900 ss Att E10 N10 O10 29 OES SSHOEHHSSESBE se do ae het B E Cm Gm du Ku m MM On Pu du 00 2c Mi 6 b Ob EB e Ge de o o m m m Oh Fh e se 0 66066666666006 se 3 0 oe D13 C15 Fh 14 OTe E13 E15 F12 FI4 F16 G13 GIS HI2 HTAHIG HIO 111 115 13 K16K14 K12L15L13 M16 M14 KT N15 K10 016 014 P15 GND C16 H9 D15 Gh E14 E16 F13 FI5 G12 G14 GI6 HIB HIS HIT HO 116 114 112 K15 K13 L16 L14 L112 MIS MI N16 N14 M12 O15 P16 R15 The letter digit code is the electrode identifier and refers to the position of the electrode in the 16 x 16 layout grid The layout of the letter digit code for the four connectors of the USB MEA256 amplifier is shown To correlate the pin layout of the connectors please see the table on the next page
146. stems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel 256 9wellMEA systems Tables Stimulation Electrode ID 1D Socket 1D o i AS1 Conn 1 No 7 BS1 Conn 1 No 36 cst Conn 2 No 8 and number A12 Conn 1 No 12 B12 Conn 1No 40 C12 Conn 2 No 12 i i A13 Conn 1 No 10 B13 Conn 1 No 38 C13 Conn 2 No 10 of sti mulation A14 Conn 1 No 5 B14 Conn 1 No 33 c14 Conn 2 No 5 connector socket A21 Conn 1 No 14 B21 Conn 1 No 42 C21 Conn 2 No 14 A22 Conn 1 No 11 B22 Conn 1 No 39 c22 Conn 2 No 11 in well A B and C A23 Conn 1No 9 B23 Conn 1 No 37 C23 Conn 2No 7 A24 Conn 1 No 6 B24 Conn 1 No 34 C24 Conn 2 No 6 A25 Conn 1 No 3 B25 Conn 1 No 31 c25 Conn 2 No 3 A31 Conn 1 No 16 B31 Conn 1 No 44 c31 Conn 2 No 16 A32 Conn 1 No 13 B32 Conn 1 No 41 c32 Conn 2 No 13 A33 Conn 1 No 8 B33 Conn 1 No 35 cs1 Conn 2 No 9 A
147. sterile distilled water Let MEAs air dry over night on a sterile workbench laminar flow with UV light turned on Coating with Fibronectin Fibronectin is a more biological coating alternative especially used for heart tissues The adhesion tends to be very stable which allows longer cultivation times Materials Fibronectin BD BioCoat M Fibronectin Cellware BD Biosciences Fibronectin solution Prepare a stock solution of 1 mg ml fibronectin in distilled water or PBS phosphate buffered saline and store it at 4 C The stock solution is diluted with water or PBS to a final concentration of 10 ug ml before use 41 5 4 7 4 4 4 4 MEA Manual Procedure 1 Cover the MEA surface with 300 ul fibronectin solution and incubate the MEA at 37 C for at least 1 h 2 Aspirate the solution and rinse the MEA 2 x with PBS phosphate buffered saline 3 Plate the cells onto the MEA immediately after coating Literature Ulrich Egert Thomas Meyer 2004 Heart on a Chip Extracellular multielectrode recordings from cardiac myocytes in vitro Methods in Cardiovascular Research S Dhein and M Delmar eds Coating with Collagen Coating with collagen is useful for short term cultures It tends to detach from the surface if used for long term cultures Materials DMEM Dulbecco s Modified Eagle Media DMEM F12 Gibco Invitrogen 21331 020 N Hydrochloric acid pH 3 0 Acid soluble type I collagen solution 3
148. t autoclave or sterilize perforated MEAs by heat These MEA types are not thermo resistant and will be irreversibly damaged Sterilization with Ethanol and UV Light Rinse MEAs with 70 ethanol Do not immerse the MEA into the alcohol for a longer time otherwise the ring on the MEA will probably get off Let MEAs air dry over night on a sterile workbench laminar flow hood with UV light turned on Steam Sterilization Autoclavation Autoclave MEAs at 125 C for 15 min Dry Heat Sterilization Dry heat sterilization is possible if a stream autoclave is not available which is the better choice Please clean the MEA thoroughly with distilled water before using the oven otherwise potential rests of any material may burn into the glass carrier of the MEA and may destroy the electrodes Thermally sterilize MEAs in an oven at 121 C for 15 min Thermally sterilize FlexMEAs in an oven at 121 C for 15 min Thermally sterilize pMEAs in an oven at 50 C for 2 hours Sterilization with Hot Water Expose MEAs to hot water 90 C for 1 min 36 MEA Manual 5 3 MEA Storage To maintain a hydrophilic surface after hydrophilization it is recommended to store the MEAs filled with water until use Dry MEAs will get hydrophobic again after some time Store MEAs filled with sterile distilled water at 4 C in the dark that is in the fridge to prevent microbiological contaminations to maintain a hydrophilic surface 5 4 MEA Coati
149. t connected to ground Connect the internal or external bath electrode to one of the ground inputs of the amplifier AgCI bath electrode needs is not well chlorided Rechloride the electrode or use a new one 50Hz hum 50 Hz is the frequency of mains power in Europe If the shielding and grounding of the setup is not sufficient electrical signals are picked up from the environment Use a proper shielding For example you can place aluminum foil over the amplifier that is connected to any metal part of the MEA amplifier You can also use special shielding equipment like a Faraday cage The following screen shot shows a recording of a 60MEA200 30 without bath electrode and without shielding You see that the signals are so high that the amplifier gets saturated and you see a very strong 50 Hz hum imi 50 Hz Hum without ground 145 000sec Xs 1000 ms gt 00 pH 1400 145th sweep Ys 1000 p WV 10000 3 10000 1000 100 silt 0 1000 nnn rail jj i Ij DECRETI OR ROO OR VIT WERT T ru JT The next pictures show the same MEA with bath electrode silver pellet but without shielding The baseline is very unsteady and oscillates with a frequency of 50 Hz EIS Lora Mm a 366 000sec xis 1000 ms gt oo HH 1400 41
150. tems com Product information is subject to change without notice LL multichannel 60pMEA100 30iR Ti 0000000000000 Electrode 31 43 42 41 52 51 53 63 61 62 71 72 73 81 33 32 22 33 83 2 21 91 3 11 101 3 a3 22 z 24 23 13 as REF x ss 15 Electrode 8 mea pins eo N N UJ O 3 5898 9s 5950 0 09 0 0 3 0 6 629 6 amp Men x R SOHO s sss 286098 2G tQ N 8 8 r c r xr N o I o 100 um 27 34 3 84 77 44 45 55 56 54 64 66 65 76 75 74 86 Electrode 06 warm Electrode The first number of the electrode number code refers to the column number the second number is the row number of the 609MEA100 30iR Ti The specified MEA pin numbers are the channel numbers that are used in the MC_Rack program when using the 2 dim source layout in Data Source Setup The electrode 14 is missing It is replaced by a big internal reference electrode and connected to MC_ Rack channel number 15 Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change German y www multichannelsystems com without notice 60pMEA200 30iR Ti 60pMEA200 30iR Ti multichannel systems e e amp e amp
151. ternal reference electrode Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60ThinMEA 60ThinMEA30 10 ITO 60ThinMEA100 10 ITO 60ThinMEA200 30iR ITO e e amp 6 6 060020 e e e e Technical Specifications GOThinMEA Temperature compatibility Dimension W x D x H Thickness Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber Multi Channel Systems w o Without ring gr Glass ring ID 19 mm OD 24 mm height 6 12 mm pr Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm pr T Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O multichannel systems 60 electrode Thin Microelectrode Array with 8 x 8 layout The electrodes are embedded in a very thin glass substrate on a robust ceramic carrier Contact pads and tracks are made from transparent indium tin oxide for high resolution imaging 0 125
152. that you have selected Configuration in the Channel Layout under Data Source Setup with a total number of 64 channels Deselect the check box Digital input channel if you do not need it otherwise it could be that one recording channel is missing In Amplifier please choose FA32I S or FA64I S and in MEA FlexMEA368 In Layout tab of the display please click Default Map a amp e The letter digit code below is the electrode identifier and refers to the position of the electrode in the grid The MC Rack channel map is constructed by looking on the back side of the electrodes because the FlexMEA electrodes are placed on the preparation upside down If you use more than one MPA3Z2I Flex and a MEA64 System the signal collector SC2x32 leads the output channels of the second amplifier to channel number 33 to 64 Please see datasheet SC2x32 for details The side with the writing NMI is the correct side with the contact pads and electrodes It might be a bit confusing that the pads look stronger from the wrong side but if you hold the FlexMEA into the light you see that the pads have a 3 dimensional appearance only from the correct side from Multi Channel Systems MCS GmbH and only for the specified purpose Warning The device may only be used together with the MPA32I Flex Damage of the device and even injuries can result from improper use Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel S
153. the MEA1060 amplifiers is it necessary to connect adapters MEA STIM ADPT INV BC for MEA1060 Inv BC amplifiers MEA STIM ADPT Up BC for MEA1060 Up BC amplifiers Stimulation MEAs are useful for example for pacing cardiac tissues like hESCM human embryonic stem cells derived cardiac myocytes that need higher voltages and durations than stimulation of neuronal tissues So the use of larger stimulating electrodes is recommended Using 60StimMEAs The same material is used for standard MEAs and 60StimMEAs Therefore they are equally robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures 19 MEA Manual 4 8 Perforated MEA 60pMEA 60pMEA200 30IR Ti 60pMEA100 30IR Ti qo d 5 Doo So oot 5 Xe A NO0 O Acute slice recordings on common glass MEAs are done from the cells at the bottom of the slice which are in contact with the MEA electrodes These cells get less oxygen and nutrients from the perfusion medium and therefore are likely to give smaller signals and might eventually die first Perforated MEAs present a solution to this problem as they allow a perfusion of the tissue from both sides at the same time thereby optimizing the oxygen supply of the acute slice Perforated MEAs are identical in size and function to the regular MEAs The recording electrodes are arranged in 8 x 8 standard layout grid in GOD MEA200 30iR Ti and in 6 x 10 layout gri
154. the provided silver wire or Ag AgCl pellet to the amplifier s ground and place it into the bath OR If you use a MEA with internal reference electrode connect the ground to the reference electrode Socket pin 15 with the provided connector Please see the manual of the respective MEA amplifier for more information about mounting MEAs and grounding 50 Recording with MEAs 7 2 General Performance Noise Level You can test a MEA before use by filling it with a standard saline buffer for example PBS phosphate buffered saline and recording the noise level of the MEA and the amplifier MEA amplifiers have a maximum noise level of 8 uV The noise level on the MEA depends on the electrode size and material The smaller the electrode the higher is the noise level TIN electrodes have a larger surface area due to their microfold structures and therefore they have generally a lower impedance and a lower noise level than electrodes of the same size that are made from other materials for example Pt electrodes The total maximum noise level for a MEA and the amplifier should be about 40 uV peak to peak for 10 uim TIN electrodes and 10 uV for 30 um TiN electrodes The initial noise level may be higher if the MEAs are hydrophobic New MEAs should be made hydrophilic before use Typical noise level of a used standard 60MEA200 30iR Ti round 30 um Tin electrodes zin 1828000sec X Axis 1000 ms gt 00
155. ubject to change Germany www multichannelsystems com without notice multichannel systems Perforated MEA for use with MEA2100 32 and USB MEA32 STIM4 System pMEA325S12 Layout 4 MC Rack channel map pMEA 32812 L4 8x4 cmp 11 14 15 29 19 23 17 18 2 13 12 4 16 31 32 22 8 10 1 6 20 30 21 26 3 5 7 9 27 28 24 25 The MC_Rack channel map is build analog to the layout of the recording electrode in the grid A29 A25 A21 A17 A13 A9 ASB At A30 A26 A22 A18 Ai4 A10 A6 A2 A31 A27 A23 A19 A15 A11 A7 A3 A32 A28 A24 A20 A16 A12 A8 AA Table Correlation of MC Rack channels and recording electrodes A Number of recording electrode CH Channel number in MC Rack S Number of stimulation electrode STG Internal stimulus generator connection REF Reference electrode S 1 STG 23 STG 16 S 7 S2 STG 25 STG 13 S8 S 3 STG 22 STG 15 S9 S4 STG 24 STG 12 S 10 S5 STG 21 STG 14 S 11 S6 STG 26 STG 11 S 12 A 19 CH 6 CH 30 A 11 A 20 CH 9 CH 31 A10 A 31 CH 8 CH 29 A17 A 21 CH 15 CH 28 A12 A 24 CH 7 CH 19 A13 A27 CH 10 CH 17 A 5 A 25 CH 14 CH 27 A16 A 28 CH 5 CH 26 A 3 A 18 CH 4 CH 18 A 1 A 29 CH 11 CH 24 A 8 A32 CH 3 CH 23 A 9 A 26 CH 13 CH 21 A 7 A 22 CH 12 CH 32 A 6 A 23 CH 1 CH 22 A 2 A30 CH 2 CH 20 A15 A14 CH 16 CH 25 A 4 Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany Fon 49 7121 9 09 25 O Fax 49 7121 9 09 25 11 info multichannelsystems com www multichannelsystems com 2013 Multi Chann
156. ultichannel systems 120pMEA200 30IR Ti Electrode Layout Table Hardware ID and Electrode ID HW ID EL ID HW ID EL ID HW ID EL ID HW ID EL ID 120 G8 90 H6 60 F5 30 E7 119 G12 89 M6 59 F1 29 A7 118 G11 88 L6 58 F2 28 B7 117 G10 87 K6 57 F3 27 C7 116 G9 86 J6 56 F4 26 D7 115 H12 85 M5 55 E1 25 A8 114 H11 84 L5 54 E2 24 B8 113 H10 83 K5 53 E3 23 C8 112 H9 82 J5 52 E4 22 D8 111 J12 81 M4 51 D1 21 A9 110 J11 80 L4 50 D2 20 B9 109 J10 79 K4 49 D3 19 C9 108 H8 78 H5 48 E5 18 E8 107 K11 77 L3 47 C2 17 B10 106 K10 76 K3 46 C3 16 C10 105 L10 75 K2 45 B3 15 C11 104 J9 74 J4 44 D4 14 D9 103 K9 73 J3 43 CA 13 D10 102 L9 72 J2 42 B4 12 D11 101 M9 71 J1 41 A4 11 D12 100 J8 70 H4 40 D5 10 E9 99 K8 69 H3 39 C5 9 E10 98 L8 68 H2 38 B5 E11 97 M8 67 H1 37 A5 7 E12 96 J7 66 G4 36 D6 6 F9 95 K7 65 G3 35 C6 5 F10 94 L7 64 G2 34 B6 4 F11 93 M7 63 G1 33 A6 3 F12 92 H7 62 G5 32 E6 2 F8 91 G7 61 G6 31 F6 1 F7 Multi Channel Systems Fon 49 7121 9 09 25 0 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice 60 4QMEA1000 60 4QMEA1000iR Ti Four Quadrants Microelectrode Array 1000 pm O e 2 200 um wr ooz wri ooz N 3 3 NJ e e t 3 O00 00000 eeoc Technical Specifications 60 4QMEA1000 Temperature compatibility Dimension W x D x H B
157. weeks and even months The electrode impedance ranges about 30 kO Tracks are made of titanium Ti and contact pads are made of titanium nitride TiN insulation material is silicon nitride Important MEAs are not symmetrical That is why the writing for example NMI LEITER MEA type on the MEA chip should be on the right side viewed from the front with the sockets of the amplifier in the back MEAs with one big internal reference electrode should be placed with reference electrode to the left side in the amplifier Otherwise the MEA layout will not match with the pin layout of the channel map in the data acquisition program Using 60SquareMEAs MEAs with square electrodes can be used for a wide variety of applications They are robust and heat stabilized They can be autoclaved and coated with different procedures for cell and tissue cultures Generally they can be used for acute experiments as well as long term cultures 28 MEA Manual 4 17 PEDOT CNT MEAs 60PedotMEA 60PedotMEA200 30iR Au Carbon nanotube stucture of PEDOT electrodes 60PedotMEAs have like standard MEAs 59 electrodes and one reference electrode that are arranged in an 8 x 8 layout grid with electrode diameters of 30 um and interelectrode distances of 200 um The flat round electrodes are made of PEDOT CNT carbon nanotube poly 3 4 ethylene dioxythiophene Contact pads and track material is made of titanium nitride TiN covered by a layer of gold
158. xaMEAs featuring a hexagonal layout perfect for recording from retina ThinMEAs with a thickness of only 180 um ideally suited for high resolution imaging Very cost efficient and robust ECOMEAs on glass or PCB printed circuit board base for applications with lower spatial resolution and higher throughput especially for established cardiomyocyte cultures large slices or whole heart preparations Stimulation MEAs with 16 additional stimulation electrodes Perforated MEAs allow perfusing the acute slice from up and downside For use with MEA2100 headstages equipped with perfusion element MEA2100 PE and MEA1060 amplifiers with perfusion ground plate MEA PGP or with Small perforated MEAs with 32 recording and 12 stimulation electrodes specified for use with MEA2100 32 and USB MEA32 STIMA System 6 well MEAs feature a round MEA layout separated in six segments of 3 x 3 electrodes like a pie chart Using the 6 well MEA with macrolon triangle or round chamber ring you have 6 separate culture chambers on one MEA for example for drug application in a screening experiment 4 quadrant 1000 MEAs with electrode layout organized in four quadrants and a center line 256MEAs with 252 recording electrodes in a 16 x 16 layout grid for use with USB MEA256 System 6 well MEAs for use with USB MEA256 System 252 electrodes in 6 blocks of 6 x 7 electrodes in a round layout for use with 6 well macrolon triangle or round chamber rings You have 6 sep
159. y Dimension W x D x H Base material Track material Contact pads Electrode diameter Interelectrode distance centre to centre Electrode height Electrode type Isolation type Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes MC Rack Source layout in Data Source Setup Channel map MEA perfusion chamber w o gr pr pr T Multi Channel Systems MCS GmbH Aspenhaustrasse 21 72770 Reutlingen Germany 0 125 C 49 mm x 49 mm x 1 mm Glass Ti Titanium TiN Titanium nitride 10 or 30 um 500 um Planar TiN Titanium nitride Silicon nitride 500 nm PEVCD 30 50 kO for 30 um electrodes 250 400 KQ for 10 um electrodes 6x 10 59 1 internal reference electrode iR 2 dim MEA MEA 500 6x10 cmp Without ring Glass ring ID 19 mm OD 24 mm height 6 12 mm Plastic ring without thread ID 26 5 mm OD 30 mm height 6 3 mm Plastic ring with thread ID 26 mm OD 30 mm height 6 15 mm Fon 49 7121 9 09 25 O Fax 449 7121 9 09 25 11 O 2013 Multi Channel Systems MCS GmbH info multichannelsystems com www multichannelsystems com Product information is subject to change without notice m multichannel 60MEA500 10iR Ti systems Electrode layout grid 6 x 10 maris O 62 6 69 3 6 6 62 6 69 69 65 6 M KI H2 K2 13 K3 H3 H4 KA 14 I5 K5 H5 K6 16 Electrode 2 e O v 6 6 9 vomm 2 H1 MEA pi
160. y and look golden If they are gray or if they show a film you should clean them Carefully clean the electrodes with a swab and distilled water under microscopic control Cleaning of EcoFlexMEAs EcoFlexMEAs made of polyimide Kapton have a temperature range from 0 125 C They can be sterilized by autoclavation If necessary carefully clean the electrodes with a swab and distilled water under microscopic control 43 5 5 5 5 5 6 5 5 7 MEA Manual Cleaning of FlexMEAs FlexMEAs made of polyimide foil have a temperature range from 10 125 C They can be sterilized by autoclavation Please do not use an ultrasonic bath for FlexMEAs Rinse with distilled water first optional with ethanol 70 Removing Nitrocellulose Coating Note It is very important that you clean MEAs that have been coated with nitrocellulose and remove all biological material first before removing the coating If you applied methanol on an uncleaned MEA you would rather fix the cell debris on the MEA than actually remove the coating 1 Directly after usage biological material is rinsed off under running water and the MEA is cleaned with pH neutral cleaning agents or enzymatically if necessary 2 Rinse the MEA 2 x with methanol If nitrocellulose is not sufficiently removed by rinsing incubate the MEA filled with methanol for 15 to 30 min to dissolve the cellulose nitrate 3 Rinse the MEA with distilled water MEA Cleaning with
161. ype Electrode impedance Electrode layout grid Number of recording electrodes Number of reference electrodes Number of ground electrodes MC_Rack Source layout in Data Source Setup Channel Map Cleaning 2200 350 500 18500 10 40 C 31 mm x 18 5 mm 12 um lt 1 g Polyimide 2611 foil Gold 30 um 300 um Diameters of the holes 30 um Planar TiN electrodes Titanium nitride Polyimide 2610 foil Approximately 50 kQ 6x6 32 2 internal reference electrodes 2 Configuration 64 channels Amplifier FA64I S or FA32I S MEA FlexMEA36 FlexMEA36 cmp Default Map in Layout tab of Data Display Rinse with distilled water optional with ethanol 70 A Do not autoclave or sterilize FlexMEAs by heat These MEA types are not heat stable and will be irreversibly damaged Please do not use ultrasonic bath for cleaning Multi Channel Systems Fon 49 7121 9 09 25 O 2013 Multi Channel Systems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice multichannel systems FlexMEA36 Electrode Layout KII T O e Gn amp ee 69 amp 69 ow Gs e w 3 Ou 00 um 6 D e 9 The numbers in the electrodes are the recording channel numbers that refer to the channel numbers in the MC Rack program Please make sure
162. ystems MCS GmbH MCS GmbH Fax 49 7121 9 09 25 11 Aspenhaustrasse 21 72770 Reutlingen info multichannelsystems com Product information is subject to change Germany www multichannelsystems com without notice FlexMEA72 multichannel systems Flexible Microelectrode Array with 72 electrodes for use via ADPT FM 72 adapter with two 32 Channel Miniature Preamplifier MPA32I for in vivo and in vitro applications Technical Specifications FlexMEA72 Temperature compatibility Dimension W x D of the FlexMEA72 Thickness of the electrode field Weight Base material Contact pads and track material Electrode diameter Interelectrode distance centre to centre Perforation Electrode height Electrode type Isolation type Electrode impedance 10 40 C 42 mm x 12 mm 12 um 19g Polyimide 2611 foil Gold 100 um 625 to 750 um Diameters of the holes 100 um Planar TiN electrodes Titanium nitride Polyimide 2611 foil Approximately 50 KQ Electrode layout grid 9x8 Number of recording electrodes 64 Number of reference electrodes 4 internal reference electrodes Number of ground electrodes 4 MC Rack Version 4 1 1 and higher Data Source Setup Source Layout Configuration Total Number of Channels 64 Amplifier FA64I S MEA FlexMEA72 Channel map Display Tab Layout gt Default Map FlexMEA72 cmp Cleaning Rinse with distilled water optional with ethanol 70 and will be irreversibly damaged Please do not us

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